The ProCell range can increasemitotic index significantly, improvechromosome length, morphology andspreading, shift cytoplasm and successfully break upblood clots - all of this on a variety of sample types.

Anti-Clotting Reagent (ACR)GGS - JL001

ACR reduces culture failure rates and thereforethe requirement for repeat sampling in bothblood and bone marrow samples.

ACR is added to a clotted sample and a shortprotocol is followed prior to setting up in culture.90% of clotted bone marrow/blood samples aresuccessfully cultured following treatment with ACR.

ACR has been found to be very effective for thefast and safe removal of maternal blood clotsfrom CVS. CVS treated with ACR have been foundto be ready for harvest an average of two daysearlier than normally treated control samples.

Chromosome Resolution Additive (CRA)GGS - JL003

CRA is a simple-to-use product that increaseschromosome resolution and is safer than ethidiumbromide. A working solution of CRA is simplyadded to the culture up to 90 minutes prior tometaphase arrest.

This product has been shown to increase QAband level on different cytogenetics sample types.CRA is a colchicine antagonist, this means that itreduces the chromosome shortening effect ofcolchicine. CRA can also be used alongsideethidium bromide for extra chromosome length.

Comparison of chromosome pairs from the same AML cellline, one with CRA added prior to metaphase arrest

1 without CRA 1 with CRA

Archival fixed bone marrow preparationslabelled with Cytocell XY

Dual label FISH probe. Images courtesy of Cytocell Ltd

Without Cytoclear With Cytoclear

CytoclearGGS - JL004

Cytoclear is an effective, easy-to-use productthat improves the quality of both brightfield andFISH samples by removing troublesomecytoplasm. Cytoclear is incorporated into the fixstage of the harvest, usually requiring littlechange to existing procedures and isappropriate for both freshly fixed and archivedfixed suspensions. Cytoclear can be used torescue cultures that have harvested badly.

Metaphase Arresting Solution (MAS)GGS - JL008

Metaphase arresting solution is a gentle alternativeto colchicine/colcemid and contains colchicine andvinblastine sulphate. This means that it is less toxicto cells because the concentration of colchicine isreduced whilst the extrablocking activity ofvinblastine sulphate means that it is extremelyeffective. Rounded-up cells in culture are much lessto likely to lift off when treated with MAS and MASmay be used as a direct replacement forcolchicine/colcemid without major alteration toexisting laboratory protocols.

Erythrocyte Lysis Solution (ELS)GGS - JL009

ELS works by lysing red blood cells, sometimesfound in amniotic fluid and bone marrow samples.These can prohibit growth by restricting colony size.ELS gently lyses the red blood cells without beingtoxic to the cells that are to be cultured. ELS canalso be used on bone marrow cultures.

All ProCell products are designed for

cytogeneticists to resolve the very real

problems encountered within both

clinical and research laboratories in their

day-to-day work, and are designed to f it

neatly into existing laboratory protocols.

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ProCell Culture Growth Supplements

Our range of cell culture growth supplement productssignificantly improve cell growth rates in specific sample types.This means mitotic index may be increased by at least 100% .A marked improvement in chromosome morphology is alsoobserved. These supplements also reduce culture failure rates,reducing the requirement for repeat sampling on poorsamples. All of our Growth Supplements are very easy to use,being simply added to the laboratory's existing culturemedium for that specific sample type.

Bone Marrow Growth Supplement (BMGS)GGS - JL002

Lymphocyte Growth Supplement (LGS)GGS - JL002/L

!NEW PRODUCTS!!

Skin Growth SupplementGGS - JL0012

Solid Tumour Growth SupplementGGS - JL0010

Lymphoma Growth SupplementGGS - JL0011

POC Growth SupplementGGS - JL0013

ProCell Hypotonic SolutionsGGS - JL005 Optimal Hypotonic SolutionGGS - JL006 Buffered Hypotonic Solution

There are two ProCell hypotonics. These improved hypotonic solutions were developed to assist in spreading chromosomes from metaphases ofchallenging sample types such as solid tissues or bone marrow samples. They consist of balanced formulations of salts to burst open cells whilstmaintaining a high degree of cohesion and fluidity within the metaphase, giving rise to superior chromosome spreading.

Use of different Hypotonics on a 47,XY,+21 Lymphoblastoid cell line.

BHS – BufferedHypotonic Solution

KCL – PotassiumChloride Solution

OHS - OptimalHypotonic Solution

Genial Genetic Solutions Limited

P.O. Box 3751 l Chester l Cheshire CH1 9UF l UKt +44 1244 404970 f +44 (0)1244 404971e [email protected] www.genialgenetics.com

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