Morphology and types of Cells in
Culture
LECTURE OF SUBJECT:
Dr. sharafaldin Al-musawi
College of Biotecholgy
LECTURE: 3SUBJECT: Animal Tissue culture
LEVEL: 4
• Fibroblast-like
• Epithelial-like
• Lymphoblast-like
Based on shape and appearance: 3 types
Fibroblast-like cells are bipolar or multipolar, have elongated shapes, and grow attached to a substrate
Morphology of Cells in Culture
•Epithelial-like cells are polygonal in shape with more regular dimensions, and grow attached to a substrate in discrete patches.
Morphology of Cells in Culture
•Lymphoblast-like cells are spherical in shape and usually grown in suspension without attaching to a surface.
Morphology of Cells in Culture
Anchorage-dependent
Anchorage-independent
Morphology of Cells in Culture
Depending on the adherence property : 2 types
Anchorage-dependent (adherent or monolayer culture):
Must be cultured while attached to a solid or semi-solid substrateExample: MCF7, PC-3
Anchorage-Independent (suspension culture):
Can be grown floating in the culture mediumExample: K562
Morphology of Cells in Culture