©2017 Waters Corporation 1 Xevo TQ-XS: Targeted Screening food and environmental analyses Warren B Potts Senior Director Americas Marketing [email protected]
©2017 Waters Corporation 1
Xevo TQ-XS: Targeted Screening food and environmental
analyses
Warren B Potts
Senior Director Americas Marketing
©2017 Waters Corporation 2
Greater sensitivity required as MRLs/ MRPLs lower and matrix complexity increases
MRL = Maximum Residue Level
MRPL = Maximum Required Performance Level
©2017 Waters Corporation 3
Screening Definitions
Non-targeted screening
– What’s in this sample?
o Known knowns
o Known unknowns
o Unknown unknowns
Targeted screening
– Here is my list, are any of them in there?
– If so, how much?
©2017 Waters Corporation 4
Expanding the Scope of
Ultimate Sensitivity Analysis
©2017 Waters Corporation 5
Key improvements to optimise performance for challenging
compounds
Segmented quadrupolesecond stage –focussed ion beam
Horizontal plates provide more controlled extraction of ions from gas flow – less energetic collisions
RF voltage constant improving robustness while mass switching
Wider profile first stage, more space for ion cloud
©2017 Waters Corporation 6
Data for a diverse mix of compounds
Xevo TQ-XS versus Xevo TQ-S
Improvement
Compound Mode Peak Area S/N
Amphetamine ESI+ 54.3 13.3
MDA ESI+ 11.4 4.4
MDMA ESI+ 5.2 1.4
MDEA ESI+ 4.3 1.7
Methylon ESI+ 3.5 1.5
Omeprazole ESI+ 1.6 1.5
Chloramphenicol ESI- 3.2 2.6
Chlorpropham ESI- 6.9 7.3
Dicamba ESI- 29.0 6.2
Dinocap ESI- 1.2 1.2
Bentazon ESI- 1.2 1.2
Triforin ESI- 1.1 1.1
Ioxynil ESI- 1.0 1.0
Fludioxonil ESI- 1.2 1.1
Dithianon ESI- 1.2 1.1
Fluazinam ESI- 1.1 1.1
Hexaflumuron ESI- 1.2 1.1
Courtesy of Gordon Fujimoto, James Morphet, Mark Halifax, Sarah Dowd, Simon Kennan Evans
©2017 Waters Corporation 7
Multi residue and multi class analysis
Multi class analytes in cereals
Screening methodology for improved lab efficiencies
http://ec.europa.eu/food/plant/pesticides/eu-pesticides-database/public/?event=homepage&language=EN
www.commodityregs.com
©2017 Waters Corporation 8
Sample Preparation
Experimental: Sample Prep and LC
Analytical column: 2.1 x 100 mm BEH C18 1.7 µm
Column temp: 45 °C
Sample temp: 4°C
Mobile phase A: 0.1 % formic acid in water
Mobile phase B: 0.1 % formic acid in methanol
Sample Man. wash: 50:50 Water/Methanol
Purge wash: 90:10 Water/Methanol
Seal wash: 90:10 Water/Methanol
Flow rate: 0.45 mL/min
Injection volume: 1 µL
Time (min) A (%) B (%) Curve
0 98 2 6
0.25 98 2 6
12.25 1 99 6
13.0 1 99 6
13.01 98 2 6
17 98 2 6
0.5 g homoginisedsample
QuEChERS CEN to 6 ml
Centrifuge
Collect extract and analyse
©2017 Waters Corporation 9
MS: Xevo TQ-XS
Capillary voltage: 1 kV
Desolvation Temp: 550 ° C
Source Temp: 150 °C
Desolvation gas: 1000 l/hr
Cone gas: 150 l/hr
MRM transitions: Method generated in QuanPedia, using Xevo TQ-S transitions
and source parameters
Experimental: MS conditions
+XS
©2017 Waters Corporation 10
Xevo TQ-XS QuanPedia
• 23 Analysis methods• 400 + compounds • Method creation in only minutes• LC, MS and TargetLynx XS methods
Bisphenol
Methylamino alanine
Paralytic shellfish toxins
PFAAS Pyrrolizidine alkaloids
Antibiotics
Binding agents
Parabens
Indoleamine neurotransmitters
Clenbuterol
Veterinary drugs
Imidazole and phenicolsPigments
Neurotoxins
PFCAS
Paraquat
Mycotoxins
Pesticides
©2017 Waters Corporation 11Time
2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00 7.50 8.00 8.50 9.00 9.50 10.00 10.50
%
0
100
Multi class and multi residue analytes:Red corn at varying MRLs
CompoundConcentration
µg/kgAF B1 6.25AF B2 1.25AF G1 3.75AF G2 1.25OTA 50DON 500Dinotefuran 10 Linuron 10Methomyl 10Picoxystrobin 10
150 cmpds; 12 mycotoxins, remainder pesticides
©2017 Waters Corporation 12
RADAR: Understanding sample complexity
Time3.60 3.80 4.00 4.20 4.40 4.60 4.80 5.00 5.20 5.40 5.60 5.80 6.00 6.20 6.40 6.60 6.80 7.00 7.20 7.40 7.60 7.80 8.00 8.20 8.40
%
0
100
Full scan data along with MRM
©2017 Waters Corporation 13
Compound name: Fenoxycarb
Correlation coefficient: r = 0.997931, r^2 = 0.995866
Calibration curve: 40584.9 * x + 2415.77
Response type: External Std, Area
Curve type: Linear, Origin: Exclude, Weighting: 1/x, Axis trans: None
Conc-0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0
Re
sp
on
se
-0
200000
400000
Conc
Re
sid
ua
l
-0.0
20.0
min8.650 8.700 8.750 8.800 8.850 8.900 8.950 9.000 9.050 9.100
%
0
100
F76:MRM of 2 channels,ES+
302.1 > 116.1
MRA_2016MAY29_160 Smooth(Mn,1x2)
Barley QC 3
7.159e+006Fenoxycarb
297076.84
min
%
0
100
F76:MRM of 2 channels,ES+
302.1 > 88
MRA_2016MAY29_160 Smooth(Mn,1x2)
Barley QC 3
9.567e+006Fenoxycarb
396866.09
From screening to quantitation & confirmation
Compound name: Aflatoxin B1
Correlation coefficient: r = 0.999388, r^2 = 0.998776
Calibration curve: 953.665 * x + 7.27701
Response type: External Std, Area
Curve type: Linear, Origin: Exclude, Weighting: 1/x, Axis trans: None
Conc-0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0
Re
sp
on
se
-0
5000
Conc
Re
sid
ua
l
-10.0
0.0
min5.800 5.850 5.900 5.950 6.000 6.050 6.100 6.150 6.200
%
0
100
F84:MRM of 2 channels,ES+
313.2 > 285.1
MRA_2016MAY29_162 Smooth(Mn,1x2)
Red Corn QC 3
5.634e+0056.19
Aflatoxin B1
9737.54
5.805.89 6.13
min
%
0
100
F84:MRM of 2 channels,ES+
313.2 > 241.1
MRA_2016MAY29_162 Smooth(Mn,1x2)
Red Corn QC 3
2.617e+005Aflatoxin B1
9339.19
6.19
©2017 Waters Corporation 15
Repeatability in green tea extract
©2017 Waters Corporation 16
Expanding the scope of ultimate sensitivity
while maintaining Xevo unique flexibility
©2017 Waters Corporation 17
Compatibility across the board
Universal Source platform
Chromatographic versatility
UPC2H-Class and I-Class M-Class
ESIAPCIESCi™
APPIAPCI
APGC ionKey/MS ASAP NanoESI UniSpray
©2017 Waters Corporation 18
Pushing the boundaries with APGC
Time8.60 8.70 8.80 8.90 9.00 9.10 9.20 9.30 9.40 9.50 9.60 9.70 9.80 9.90 10.00 10.10 10.20 10.30 10.40 10.50 10.60 10.70 10.80 10.90 11.00 11.10 11.20 11.30 11.40 11.50
%
0
100
XEVO TQ-XS
XEVO TQ-S
> 5 X ↑ S:N
Time7.50 7.60 7.70 7.80 7.90 8.00 8.10 8.20 8.30 8.40 8.50 8.60 8.70 8.80 8.90 9.00 9.10 9.20 9.30 9.40 9.50 9.60 9.70 9.80 9.90 10.00 10.10 10.20
%
0
100
500 ag TCDD (on column)
S:N 19:1 (PtP 2SD)
Ion transition ratio 5.5 %
Courtesy of Dave Douce, Rhys Jones
©2017 Waters Corporation 19
Pushing the boundaries with APGC
Sub-fg sensitivity for TCDD for the first time
– Reproducible injections of 500 ag on column
– S/ N for all > 10Area S/N
Ion Ratio
1. 35 11 0.913
2. 38 13 0.872
3. 40 18 1.038
4. 39 20 1.006
5. 37 18 1.021
Courtesy of Dave Douce, Rhys Jones
©2017 Waters Corporation 20
Compatibility across the board
Universal Source platform
Chromatographic versatility
UPC2H-Class and I-Class M-Class
ESIAPCIESCi™
APPIAPCI
APGC ionKey/MS ASAP NanoESI UniSpray
©2017 Waters Corporation 21
A novel ionisation source:UniSpray
A novel ionisation source for broader
compound coverage
Increased ionisation efficiency
• Creation of small volume secondary
droplets through impact and shear
stress
• Enhanced desolvation and droplet
evaporation Target(High Voltage)
Grounded Probe
Sample Cone
©2017 Waters Corporation 22
UniSpray: Pesticides in Green Tea Extract
UniSpray S:N
Improvement
UniSpray Peak Area
ImprovementMethamidophos 1.9 5.7
Acephate 1.3 1.7Methomyl 0.9 1.5
Dicrotophos 1.4 3.5Atrazine desethyl 0.8 4.6
Metoxuron 1.6 4.1Cyanazine 1.1 4.4Simizine 1.5 3.6
Hexazinone 0.4 2.8Monolinuron 1.5 3.6Chortoluron 2.5 4.1
Metobromuron 1.9 3.8Atrazine 3.0 4.3Diuron 1.7 4.7Linuron 1.9 4.0
Sebuthylazine 3.1 4.4Terbuthylazine 2.4 3.9
Buprofenzin 1.8 3.2Fenpropimorph 2.5 2.4
©2017 Waters Corporation 23
Time2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00 7.50 8.00 8.50 9.00 9.50 10.00 10.50
%
0
100
Summary
Comprehensive family of advancing technologies to suit your analytical
needs
Selective and sensitive MS/MS methods for comprehensive routine
screening to confirmatory multi residues methods
Robust and accessible tandem quadrupole MS technologies
Improved supporting tools to aid in method development, transfer and
extension to scope
©2017 Waters Corporation 24