XBRIDGE AMIDE COLUMNS HYDROPHILIC INTERACTION CHROMATOGRAPHY Since 2003, Waters has developed innovative stationary phases for Hydrophilic Interaction Chromatography [HILIC] to overcome the challenge of retaining and separating extremely polar compounds. Based on Waters novel ethylene bridged hybrid [BEH] particle technology, NEW XBridge ™ Amide columns utilize a chemically stable, trifunctionally-bonded amide phase, enabling a new dimension in stability and versatility for HILIC separations. XBridge Amide columns offer the same selectivity as ACQUITY UPLC ® BEH Amide columns, allowing scientists to transfer their separations between HPLC and UPLC ® technology platforms. Designed to retain polar analytes and metabolites that are too polar to retain by reversed-phase [RP] chromatography, XBridge Amide columns facilitate the use of a wide range of mobile phase pH [2 – 11] to facilitate the exceptional retention of polar analytes spanning a wide range in polarity, structural moiety and pKa. In addition to enhanced retention of polar compounds, XBridge Amide columns provide increased mass spectrometry response, direct compatibility with sample preparation eluates [PPT, LLE and SPE] as well as orthogonal selectivity compared to reversed-phase materials, making HILIC an attractive alternative separation technique. [ PRODUCT SOLUTION ] O Si O O O NH 2 Linker Particle Type: Ethylene Bridged Hybrid [BEH] Ligand Type: Trifunctional Amide Particle Size: 3.5 µm Ligand Density: 7.5 µmol/m 2 Carbon Load: 12% Endcap Style: None pH Range: 2-11 Pressure Tolerance: 400 bar 0.000 0.002 0.004 0.006 0.008 0.010 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 min 0.000 0.002 0.004 0.006 0.008 0.010 Ginsenoside Rb1 Ginsenoside Rb1 Orento extract 20 μg/mL Ginsenoside Rb1 standard AU AU Column: XBridge Amide, 3.5 µm, 4.6 x 150 mm Part Number: 186004869 Mobile Phase : 80/20 ACN/H 2 O Flow Rate: 1.4 mL/min Inj. Vol.: 11.5 µL Sample Diluent: MeOH Column Temp.: 60 °C Needle Wash: ACN/H 2 O 95/5 Detection: UV @ 203 nm Sampling Rate: 20 points/sec Time Constant: 0.1 Instrument: Waters Alliance ® with 2998 PDA Detector XBridge Amide Analysis of Ginsenoside Rb1
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XBridge Amide Columns - Waters Corporation Amide Columns Chemistry Particle Size Dimension Type Part Number Amide 3.5 µm 1.0 x 50 mm Column 186004871 Amide 3.5 µm 2.1 x 10 mm Guard
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X B ri dg e Am i d e Co lum ns
HydropHiliC int erACt ion CHromAtogrApHy
Since 2003, Waters has developed innovative stationary phases
for Hydrophilic Interaction Chromatography [HILIC] to overcome the
challenge of retaining and separating extremely polar compounds.
Based on Waters novel ethylene bridged hybrid [BEH] particle
technology, NEW XBridge™ Amide columns utilize a chemically stable,
trifunctionally-bonded amide phase, enabling a new dimension in
stability and versatility for HILIC separations. XBridge Amide columns
offer the same selectivity as ACQUITY UPLC® BEH Amide columns,
allowing scientists to transfer their separations between HPLC and
UPLC® technology platforms.
Designed to retain polar analytes and metabolites that are too polar
to retain by reversed-phase [RP] chromatography, XBridge Amide
columns facilitate the use of a wide range of mobile phase pH [2 – 11]
to facilitate the exceptional retention of polar analytes spanning a
wide range in polarity, structural moiety and pKa.
In addition to enhanced retention of polar compounds, XBridge
Amide columns provide increased mass spectrometry response, direct
compatibility with sample preparation eluates [ PPT, LLE and SPE] as
well as orthogonal selectivity compared to reversed-phase materials,
making HILIC an attractive alternative separation technique.