Meeting for the Advancement of Research in the Indian Ocean Region - Saint-Denis de la Réunion – 3-5 June 2003 Workshop 2 “Research on Genetic Resources and the Genome: A Need for regional Partnership. The Example of Sugar Cane” Louis-Marie RABOIN UMR PVBMT CIRAD – University of La Réunion Meeting for the Advancement of Research in the Indian Ocean Region Saint-Denis de la Réunion – 3-5 June 2003 Meeting for the Advancement of Research in the Indian Ocean Region - Saint-Denis de la Réunion – 3-5 June 2003 Research on Plant genome Biodiversity evaluation of genetic resources ⇒Management and conservation of genetic resources ⇒Valorisation of genetic resources Plant breeding ⇒New methods (MAS, GMOs…) ⇒Plant variety protection (UPOV) Seeds of Traditionnal Reunionese vegetables «légumes lontans» (M.Roux-cuvelier) Sugarcane chromosomes (A. D’Hont) 0 mS S CIR4m5 25,5 R137Sstd 25,6 aagctt19 27,2 atctt22 28,2 aaccac6 28,8 attcag 30,7 Rust resistance 32,9 actctg9r 32,9 CDS R29 32,9 aaccgt 32,9 aagcta23 33 acgctt1O 36 acgctt17 59,2 acgcag6 0 accctt16 16,5 RGA024Ss ta 19,4 RGA183Ss ta 20,3 mSSCIR52m6 24 aggcat5 26,3 aagcat23 33,5 actcat4 44 mSSCIR13m2 52,3 RGA313Ecoe 52,3 RGA313Ecob 75,2 acgcag7 Genetic map of sugarcane cultivar R 570 Maize Hybridization (J. Dintinger)
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Meeting for the Advancement of Research in the Indian Ocean Region - Saint-Denis de la Réunion – 3-5 June 2003
Workshop 2“Research on Genetic Resources
and the Genome: A Need for regional Partnership. The Example of Sugar Cane”
Louis-Marie RABOINUMR PVBMT CIRAD – University of La Réunion
Meeting for the Advancement of Research in the Indian Ocean RegionSaint-Denis de la Réunion – 3-5 June 2003
Meeting for the Advancement of Research in the Indian Ocean Region - Saint-Denis de la Réunion – 3-5 June 2003
25,5 R137Ss td25,6 aagctt1927,2 atctt2228,2 aaccac628,8 attcag30,7 Rus t res is tance 32,9 actc tg9r32,9 CDSR2932,9 aaccgt32,9 aagcta23
33 acgctt1O36 acgctt17
59,2 acgcag6
0 accctt16
16,5 RGA024Ss ta19,4 RGA183Ss ta20,3 mSSCIR52m6
24 aggcat526,3 aagcat2333,5 ac tca t4
44 mSSCIR13m2
52,3 RGA313Ecoe52,3 RGA313Ecob
75,2 acgcag7
Genetic map of sugarcane cultivar R 570 Maize Hybridization (J. Dintinger)
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Meeting for the Advancement of Research in the Indian Ocean Region - Saint-Denis de la Réunion – 3-5 June 2003
On the way back : A good knowledge of genes will facilitate the exploration, management and use of available genetic resources
Towards the localisation, identification and isolation of interesting genes
1 BAC100 kb
1 chromosome100 000 kb
Sequence
Genetic map BAC contigs
Genome10 000 000 kb
1 Gene1-5 kb
Meeting for the Advancement of Research in the Indian Ocean Region - Saint-Denis de la Réunion – 3-5 June 2003
INTEREST OF MODEL PLANTS
recombinants
S. spontaneum
S. officinarum
Maize2n=20
5 500 Mbp
Syntenic Relationships
Sugarcane2n=c.a.115
10 000 Mbp
Arabidopsis2n =10
660 Mbp
Rice2n =24
860 Mbp
Genoplante
(Collaborative projects)
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Meeting for the Advancement of Research in the Indian Ocean Region - Saint-Denis de la Réunion – 3-5 June 2003
High throughput technologies« more markers, more samples, less time »
Experimentation
Knowledge of genetic resources
Identification of genes
Meeting for the Advancement of Research in the Indian Ocean Region - Saint-Denis de la Réunion – 3-5 June 2003
Maize
-Maize germplasm from Reunion: a unique source of resistance to viral diseases
-Study of genes involved in those resistance and introgression in adapted lines
Examples of Research Programmes conducted in Plant Protection Pole (3P)
Vanilla (project)
- Study of genetic diversity of a collection of Vanilla Planifolia (cultivated in Reunion)
Tomato
- Study of resistance to viruses and bacteria
Tomato yellow leaf curl virus
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Meeting for the Advancement of Research in the Indian Ocean Region - Saint-Denis de la Réunion – 3-5 June 2003
Sugarcane
-Sugarcane : number one crop in Reunion
-Resistance to sugarcane diseases has been a concern for CERF since its creation in 1929
-Exploration of the very complex genome of sugarcane with the objective to identify genes involved in sugarcane resistance to its different pathogens and pests
Gumming rust
Leafscald
Examples of Research Programmes conducted in Plant Protection Pole (3P)
Meeting for the Advancement of Research in the Indian Ocean Region - Saint-Denis de la Réunion – 3-5 June 2003
CSIRO,BSESAustraliaCytogenetic
mappingCIRADet Univ.Reunion(UMR PVBMT)
Genetic mappingDisease résistancesugar content
CERFBrasilESTR genesBAC sequencing
Barbados Sugar content taggingIntrogression
China
Europe, projet riz plante modelFrance, génopole, génoplante, génoscope, inra, Ird