What is Bio-technology? Why is it important to understand your genetic make up? How does this information affect your life? Society? .

What is Bio-technology?What is Bio-technology?Why is it important to understand Why is it important to understand

your genetic make up?your genetic make up?How does this information affect How does this information affect

your life? Society?your life? Society?

http://www.komonews.com/news/http://www.komonews.com/news/consumer/17071161.htmlconsumer/17071161.html

Advancement of technology for research associated with life science. Especially Genetic Engineering (changing genes and recombinant DNA (changing DNA sequences)

Eliminating crime at EHS by Eliminating crime at EHS by incorporating electrophoresis into our incorporating electrophoresis into our discipline policy. Would you like to discipline policy. Would you like to

help?help?

Tools you need to learn to use…Tools you need to learn to use…Micro pipettes… Micro pipettes… Electrophoresis…Electrophoresis…Before we can use these tools we will need training Before we can use these tools we will need training

on how they work…on how they work…

What is the metric system?What is the metric system?..

How big is a Liter?How big is a Liter? How big is a mili Liter?How big is a mili Liter? mL(1/1000 L) mili means one thousanthmL(1/1000 L) mili means one thousanth How big is a micro liter compared to a L and a How big is a micro liter compared to a L and a

mL?mL? (1/1000 of a mL) (1/1,000,000 of a L)(1/1000 of a mL) (1/1,000,000 of a L) Which of these units do you think would be the Which of these units do you think would be the

best for looking at pieces of DNA?best for looking at pieces of DNA? Why?Why?

Standard measurement system Standard measurement system used through out the worldused through out the world

The MICRO LITER!!The MICRO LITER!!

DNA IS SO SMALL THAT IT NEEDS TO DNA IS SO SMALL THAT IT NEEDS TO BE LOOKED AT IN VERY TINY PIECESBE LOOKED AT IN VERY TINY PIECES

What tool should we use to measure DNA?What tool should we use to measure DNA? Micro-pipets allow us to take samples as Micro-pipets allow us to take samples as

small as .5 small as .5 µL and as large as 1000 µL (1 µL and as large as 1000 µL (1 mL)mL)

Are you a metric system master?Are you a metric system master?

When I convert from L to mL will the number get When I convert from L to mL will the number get smaller or larger? smaller or larger?

Larger, I will move my decimal place 3 spaces to Larger, I will move my decimal place 3 spaces to the right because it is a thousand times smaller.the right because it is a thousand times smaller.

When I convert from mL to When I convert from mL to µL will the number µL will the number get smaller or larger?get smaller or larger?

Larger, I will move my decimal place 3 places to Larger, I will move my decimal place 3 places to the right.the right.

Metric System Master?Metric System Master? When I convert from When I convert from µµL to mL will the number L to mL will the number

get smaller or larger?get smaller or larger? Smaller, I will move my decimal place 3 places to Smaller, I will move my decimal place 3 places to

the left. For example 2 the left. For example 2 µµL = .002 mLL = .002 mL

When I convert from mL to L will the number get When I convert from mL to L will the number get smaller or larger?smaller or larger?

Smaller. I will move my decimal place 3 spaces to Smaller. I will move my decimal place 3 spaces to the left.the left.

2.0 mL = .002 L 2.0 mL = .002 L

Factor Label MethodFactor Label Method

A great way to set up conversionsA great way to set up conversions Start the conversion with what you know and Start the conversion with what you know and

make a relationship that relates to what you want make a relationship that relates to what you want to knowto know

For example when determining how many For example when determining how many µL are in 20 L set up this conversionµL are in 20 L set up this conversion

20L 20L 1,000,000 µL 1,000,000 µL 1 L1 LThis means 20 X 1,000,000 =20,000,000 This means 20 X 1,000,000 =20,000,000 µLµL

Metric System Master???Metric System Master???

If I have L and want If I have L and want µL what should I do?µL what should I do? Move the decimal place 6 places to the rightMove the decimal place 6 places to the right Or set a conversion using factor label methodOr set a conversion using factor label method

I will give you a measurement and you must tell me how I will give you a measurement and you must tell me how many many µµL I have… Use Factor Label methodL I have… Use Factor Label method

2 2 µL = how many L?µL = how many L? 2 µL 2 µL 1 L1 L

1,000,000 µL1,000,000 µL2µL X 1L /1,000,000µL2µL X 1L /1,000,000µL==

.000002L.000002L 87 mL = how many µL?87 mL = how many µL?

87mL 87mL 1,000 µL1,000 µL 1 mL1 mL87mLx 1000 µL/ 1mL87mLx 1000 µL/ 1mL

87,000 µL87,000 µL

Try these on your own….Try these on your own…. 100 100 µL = How many LµL = How many L 2,300,000 L = How many mL2,300,000 L = How many mL 45 L = How many µL?45 L = How many µL?

1. .0001

2. 2,300,000,000mL

3. 45,000,000 µL

EQUIPMENT!!!EQUIPMENT!!!

You must show DeNA locator You must show DeNA locator competentence before you can join the competentence before you can join the EHS crime fighting team!!EHS crime fighting team!!

P-10P-10 range of .5-10 range of .5-10 µLµL P-20 range of 2- 20 µLP-20 range of 2- 20 µL P-200P-200 range of 20-200 µLrange of 20-200 µL P 1000P 1000 range of 200-1000 µLrange of 200-1000 µL

IF YOU USE THE WRONG PIPETTE IT IF YOU USE THE WRONG PIPETTE IT WILL MAKE THEM LESS ACCURATE WILL MAKE THEM LESS ACCURATE

AND MAY DESTROY THEM..AND THEY AND MAY DESTROY THEM..AND THEY MIGHT EXPLODE!MIGHT EXPLODE!

If I want to measure 1.2 If I want to measure 1.2 µL which pipette would µL which pipette would I choose?I choose?

P-10P-10 If I want to measure 178 µL which pipette would If I want to measure 178 µL which pipette would

I choose?I choose? P-200P-200 If I want to measure 778 µL which pipette would If I want to measure 778 µL which pipette would

I choose?I choose? P-1000P-1000

Try these on your own.Try these on your own.

Tell me the pipet for the specific Tell me the pipet for the specific measurement.measurement.

600 600 µLµL 25 µL25 µL 225 µL225 µL 90 µL90 µL 1 µL 18 µL1 µL 18 µL

1. P-1000 4. p-200

2. P-1000 5. P-200

3. P-10 6. P-20

Use Factor Label to solve the Use Factor Label to solve the followingfollowing

How many micro Liters are in 27 L?How many micro Liters are in 27 L?

How many mL are in 23.4 micro Liters?How many mL are in 23.4 micro Liters?

How many micro Liters are in .234 L?How many micro Liters are in .234 L?

Measure for Measure: You needMeasure for Measure: You need

Pipettes- May have to share p-10 and p-Pipettes- May have to share p-10 and p-10001000

Set of Tips for p-20/p-200 and p-10’s will Set of Tips for p-20/p-200 and p-10’s will share p-1000share p-1000

ParafilmParafilm Microtubes (3)Microtubes (3) Sample of waterSample of water

How could we determine the How could we determine the components of a mystery dye components of a mystery dye mixture using electrophoreis?mixture using electrophoreis?

Purpose: To determine the dyes in two Purpose: To determine the dyes in two mystery mixtures: XXX and ZZZmystery mixtures: XXX and ZZZ

Procedure:Procedure:MaterialsMaterials

electrophoresis apparatuselectrophoresis apparatus (BRL Horizon 58 gel box)(BRL Horizon 58 gel box) Power SupplyPower Supply (shared by two groups)(shared by two groups) beaker for used tipsbeaker for used tips 1% agarose gel1% agarose gel 1X TAE buffer (enough to 1X TAE buffer (enough to

cover gel)cover gel) 6 known dyes6 known dyes 2 mystery mixtures (XXX 2 mystery mixtures (XXX

and ZZZ)and ZZZ)

micropipet tipsmicropipet tips micropipets blot paper (1 micropipets blot paper (1

per person) per person) ziplock bagziplock bag acetate sheets (1 per person)acetate sheets (1 per person) plastic wrap marker penplastic wrap marker pen ruler (mm) microtube rack ruler (mm) microtube rack

microtubesmicrotubes

StepsSteps

See Handout:See Handout:

Data:Data: Distance traveled (in mm)Distance traveled (in mm) To which pole +/-To which pole +/- Comments & ObservationsComments & Observations

Picture of Gel Before and AfterPicture of Gel Before and After

For Tommorrow:For Tommorrow:

Finish Stuff to know before you Go: In Finish Stuff to know before you Go: In reading notebookreading notebook

Pre-Lab- Purpose,Procedure,Data SectionPre-Lab- Purpose,Procedure,Data Section

How should I take data?How should I take data?

Look at get on light boxLook at get on light box Record DNA distances on acetateRecord DNA distances on acetate Compare # of bands and distance of bandsCompare # of bands and distance of bands

Work on Conclusion after you’re Work on Conclusion after you’re done!done!

If you will not be here Monday/Tuesday or If you will not be here Monday/Tuesday or Wednesday you must keep up!Wednesday you must keep up!

We will quiz Monday/Tuesday after break We will quiz Monday/Tuesday after break as well as have a GATTACA discussionas well as have a GATTACA discussion