· Web view1Department of Anatomy and Physiology, Kansas State University, Manhattan, KS 66506. 2Department of Entomology, Kansas State University, Manhattan, KS 66506. 3USDA, Agricultural

Supplementary material

The Tribolium castaneum cell line TcA: a new tool kit for cell biology

Kristopher Silver1,*, Hongbo Jiang2,*, Junping Fu2, Thomas W. Phillips2, Richard W. Beeman2,3, and Yoonseong Park2.

1Department of Anatomy and Physiology, Kansas State University, Manhattan, KS 66506.

2Department of Entomology, Kansas State University, Manhattan, KS 66506.

3USDA, Agricultural Research Service, Center for Grain and Animal Health Research, 1515 College Ave, Manhattan, KS 66502.

*K. Silver and H. Jiang contributed equally to this work.

Table S1. Primers used in this study

Table S2: Expression of Cuticle- and Chitin synthesis-associated genes in the TcA cell line.

Table S3: Expression of immunity-related genes in the TcA cell line.

Figure S1.

The map for the plasmid used in this study. The sequence is deposited in NCBI with the GenBank Accession ############.

Table S1. Primers used in this study

Experiments Targets Primer information

Forward Reverse

PCR and cloning

pIZT backbone

pIZT-F1

*CGAATTTAAAGCTTGGTACCG

pIZT-R1

*GGGGTACCCCGCATGCCCAGACATGATAAGATACATTG

pIZT-F3

GGAAGATCTTCCGGTGAGGAACTAAACCATGG

pIZT-R5

TTGGCGCGCCAAGGAAGATCTTCCGGTCTGAC

bla-Amp blaPro

TTGGCGCGCCAATGCGCGGAACCCCTATTTG

blaAmp-R

GGAAGATCTTCCGGTCTGACAGTTACCAATGC

dTomato dTomatoF

ATGGTGAGCAAGGGCGAG

dTomatoR

TTACTTGTACAGCTCGTCCATG

TcHS promoter

KpnI-HSP-F

GGGGTACCCCGCTTGCTTAGCTTTGTTGC

KpnI-HSP-R

GGGGTACCCCTCAAATTCACTGACAGCGC

Tc006550 Promoter

6550ProF

TTGGCGCGCCAACAGCCGTTTTATTGCTTTCC

6550ProR

TTGGCGCGCCAACCTTACGTTAGAATTGAGTTACG

Tc014362 promoter

14362ProF

TTGGCGCGCCAAGGGTGCTGAACAACTTAAGTC

14362ProF

TTGGCGCGCCAATTTTCACCTTTGAGACACACA

Tc000476 promoter

476ProF

TTGGCGCGCCAAGCCCTAAATGACAAACGC

476ProR

TTGGCGCGCCAATTTGAACAAGGTAGATGTGAAC

Tcα-tubulin promoter

α-tubF1

TTGGCGCGCCAATACCGACATGGCGGGGAG

α-tubR1

TTGGCGCGCCAAACCCCGATTGTTTAGCTTGT

CMV promoter

CMVpro-F-KpnI CMVpro-R-KpnI

GGGGTACCCCGCGTTGACATTGATTATTGAC GGGGTACCCCGAGAGCTCTGCTTATATAGACC

dsRNA synthesis

TcVermillion dsTcVer-F

taatacgactcactatagggGAGCAAATCGCCAAGTCGG

dsTcVer-R

taatacgactcactatagggCCTGGGTTCGTCCCTGTAA

TcCP6 dsCP6-F

taatacgactcactatagggCGCCTTGCCATGGACTGGACC

dsCP6-R

taatacgactcactatagggCGCATCCCCGTTTTCCGGGT

Nanoluciferase dsNlucF

taatacgactcactataGGGAGGTGTGTCCAGTTTG

dsNlucR

taatacgactcactataggGTTGATCAGGCGCTCGTC

qPCR HSP68a 68-qF

ATACGAAGATAGACAGAAACAGC

68a-qR

ACTAAGTAGGAACAAAAACCGA

HSP68b 68-qF

ATACGAAGATAGACAGAAACAGC

68b-qR

CATATACAACAAAAGGATTTAAC

Tc010172 10172-qF

GTACCAACAAGGCGGTCAG

10172-qR

CAATAGTGACTGCATTTAACATAATAC

Star marks (*) are for phosphorylations at the 5’ end of the primers. Underlined nucleotides are the restriction sites introduced in the primers. Italic nucleotides are additional bases attached to end of the DNA molecule to ensure the effective cleavage close to the hangout of the PCR products. Nucleotides in lowercases are the T7 promoter used for the dsRNA synthesis.

Table S2: Expression of Cuticle- and Chitin synthesis-associated genes in the TcA cell line.

Description TC# RPKM Raw Reads

cuticle protein cp6 (TcCPR71)TC01313

5 41.58 2553

ribosomal protein s2 (TcRtv)TC00736

4 38.16 4603

protein yellow (Tcyellow-1)TC00544

4 22.04 4518

imaginal disc growth factor 4TC01391

7 13.58 2536cuticular protein analogous to peritrophins 3-a1 (TcObst-A1)

TC011140 10.91 1123

yellow protein (Tcyellow-F)TC00556

5 8.636 1699

cuticular protein ld-cp1v1TC00044

2 7.537 642

tyrosine hydroxylaseTC00249

6 6.665 1588

imaginal disc growth factor 4TC01391

8 6.192 1178

laccase 1TC00082

1 6.103 1842

integrin beta subunit (agap000815-pa) TcPSBI-1TC01170

7 4.818 1756

dopamine n acetyltransferaseTC00820

4 4.806 534

chitin deacetylase 4TC00763

5 4.428 940

yellow-bTC00548

0 4.383 796

brain chitinase and chia (chitinase20)TC00987

2 3.904 758cuticular protein analogous to peritrophins 3-b (TCObst-B)

TC011139 3.461 419

yellow-cTC01629

9 3.273 576

cuticular protein analogous to peritrophins 1-cTC00031

6 3.003 557

cuticular protein rr-1 family (agap000344-pa)TC00111

8 2.783 290


4 2.619 205


0 2.589 599

cuticular protein analogous to peritrophins 3-e (TcObst-E)

TC011349 2.294 246

domon domain-containing protein (TcKNK1)TC01065

3 2.216 643

n-acetyltransferase 2TC00790

5 2.187 208


3 1.937 484


9 1.853 258

adult cuticleTC00350

7 1.683 123

adult cuticular proteinTC01398

7 1.655 136

pupal cuticle proteinTC01477

1 1.376 94multidrug-resistance like protein isoform m (TcSUR)

TC012253 1.306 1020

syntaxin 1a (TcSyn4a)TC01169

4 1.094 157

cuticular proteinTC00989

0 1.092 94

gasp precursor (TcObst1&2)TC00116

9 1.082 291cuticular protein analogous to peritrophins 3-d2 (TC-Obst-D)

TC001350 1.03 114

syntaxin 13 (TcSyn 4c?)TC00717

7 0.9181 104

inwardly rectifying potassium isoform b (TcKir1)TC00670

6 0.8042 161

protein yellow (Tcyellow-E or Tcyellow-D)TC00622

9 0.7772 165

syntaxin-like protein (TcSyn1 or 6?)TC00307

4 0.7709 46

larval cuticle protein a3a (tm-a3a) (tm-lcp a3a)TC00072

0 0.7457 59

10g08 (TcSyn5)TC00797

4 0.7292 169

syntaxin 18TC01147

5 0.6586 84chitinase domain-containing protein 1 (chitinase 21)

TC006344 0.6005 101

domon domain-containing protein (TcSkeletor)TC01067

5 0.597 349

syntaxin 16TC00987

0 0.5947 72

brain chitinase and chia (chitinase 11N)TC01566

5 0.5017 59

cuticular protein 62bcTC00243

4 0.4942 25

cuticular protein analogous to peritrophins 1-dTC00926

3 0.481 47


6 0.4452 36

cuticular protein 62bc cg1919-paTC01381

6 0.4336 42

syntaxin 8TC00313

7 0.4292 18

syntaxin 17TC00364

8 0.4141 53

tanTC00344

8 0.4134 69

multidrug resistance-associated protein 7 (SUR2)TC00803

5 0.3788 246

cuticular protein analogous to peritrophins 1-bTC00058

7 0.3621 31


1 0.361 59

isoform d (TcCDA2)TC01410

1 0.3429 86

amp dependent ligase (Tcebony)TC01197

6 0.325 121

cuticular protein analogous to peritrophins 1-aTC00473

3 0.3093 44

udp-n-acteylglucosamine pyrophosphorylaseTC00559

3 0.3065 64cuticular protein analogous to peritrophins 3-a2 (TcObst-A2)

TC011141 0.2928 30

cuticular protein analogous to peritrophins 3-d1 (TcObst-X)

TC011142 0.2828 28

pupal cuticle protein 36aTC01313

8 0.2557 22

fused lobes (TcFDL)TC00977

9 0.2514 65

chitin deacetylase-like isoform d (CDA5)TC00684

6 0.2431 117

endocuticle structural glycoprotein bd-TC01312

6 0.2383 17

cuticle proteinTC00335

6 0.2103 56


8 0.2071 12


6 0.1821 10

cg1397-pa (TcRtv)TC00738

4 0.1775 35

aromatic amino acid decarboxylase TcDDC3)TC01340

2 0.1472 28

metalloproteinase inhibitor 3 (TcTIMP)TC00230

5 0.1452 13


9 0.1383 11


3 0.1362 11


1 0.1312 8

chitinase 3 (chitinase 16)TC00917

6 0.1201 20

brain chitinase and chia (chitinase 6N)TC00387

6 0.1191 122

yellowTC00080

2 0.1176 21

protein naked cuticle-like proteinTC00163

7 0.115 29


2 0.1088 8

chitin synthase 2TC01216

3 0.1058 67

syntaxin 1a (TcSyn4b)TC01209

4 0.1016 13


5 0.09353 11

cuticular protein 97eaTC00111

9 0.08975 13

dopa decarboxylaseTC01348

0 0.08746 18

tpa: cuticle proteinTC01289

2 0.08695 10


4 0.08131 9

yellow-hTC00623

0 0.0789 16

major royal jelly protein 4 (Tcyellow-4)TC00250

8 0.0763 13


7 0.07326 7


5 0.07265 12

major royal jelly protein 4 (Tcyellow-5)TC00250

9 0.07171 12


4 0.07116 4

cuticle protein cpg42TC00482

7 0.06704 10

chitin synthase 1TC01463

4 0.06004 42

cuticular protein analogous to peritrophins 1-hTC00989

4 0.05894 21

cuticular protein 100aTC00111

5 0.05782 5


9 0.05354 5

yellow-h cg1629-pa (Tcyellow-3)TC00389

8 0.04513 8


4 0.04303 4


2 0.03903 4

aromatic amino acid decarboxylase (TcTyrDC1)TC01256

7 0.03683 10

cuticular protein 92fTC01282

8 0.03544 4

laccase-like multicopper oxidase 1 (TcLac2)TC01049

0 0.03491 4

udp-n-acteylglucosamine pyrophosphorylaseTC00175

1 0.03359 7


3 0.03304 1


8 0.03257 2


4 0.0314 3

isoform b (TcKNK3)TC00230

4 0.03136 8

pupal cuticle protein c1b (tm-c1b) (tm-pcp c1b)TC00626

2 0.03063 2


1 0.02877 5

dopa decarboxylase (TcDDC2)TC01340

1 0.02764 6

glutamate decarboxylaseTC00932

4 0.0271 6


9 0.02224 1

chitin-binding domain containing proteinTC00450

0 0.02192 2

beta-n-acetylglucosaminidase nag2TC01154

0 0.02065 5

cuticular protein 47ef cg13214-paTC00259

5 0.01994 2


9 0.01994 1


0 0.01944 1

laccase-like multicopper oxidase 1 (TcLac2)TC01048

9 0.01933 6

beta nu integrin subunit (TcPSBI-3)TC00578

2 0.01891 6

cuticular protein 50cbTC00698

1 0.0188 5


9 0.01828 4


8 0.01752 1

cuticle protein cp5TC01472

0 0.01739 1


8 0.0172 2


5 0.01701 1


6 0.01652 1


0 0.01617 1

cg34355 cg34355-pa (TcKNK2)TC01230

1 0.01608 5

cdc42 gtpase-activating protein (TcPMP1&2)TC00609

8 0.01596 15


9 0.01584 1


8 0.01522 1


8 0.01492 1


0 0.01445 2

cysteine sulfinic acid (TcDc CG5618)TC01417

7 0.01439 3

brain chitinase and chiaTC00317

9 0.01385 1


0 0.01384 2

protein yellow (Tcyellow-2)TC00353

9 0.01345 2

chitinase (chitinase 5)TC00177

0 0.01299 3


5 0.01299 1

chitin deacetylase 1 (CDA8)TC01414

7 0.01227 2

cuticular protein 50cbTC00401

0 0.0118 1

chitinase 7TC01548

1 0.01174 5

integrin beta-ps (TcPSBI-2)TC01370

6 0.01171 4


9 0.01134 1


5 0.01134 3


2 0.01056 1

chitin binding proteinTC01356

8 0.0101 2

cuticleTC00085

1 0.009926 1


7 0.009926 1

cuticular protein analogous to peritrophins 1-gTC00887

7 0.0098 1


6 0.00944 1


0 0.008964 1


6 0.008964 1

hypothetical protein TcasGA2_TC016350TC01635

0 0.008349 1


1 0.006934 1

inwardly rectifying k+ (Kir2)TC00119

9 0.006723 1


1 0.006497 1

peritrophic matrix protein 14 (TcPMP5)TC00327

3 0.0062 1


2 0.005725 1


8 0.005468 1

cuticular protein cpg12TC00698

5 0.005305 2

cuticular protein analogous to peritrophins 1-jTC01110

1 0.005272 3

ionotropic glutamate receptor-invertebrateTC01630

0 0.004931 1


5 0.004798 1

inwardly rectifying k+ (TcKir3)TC00670

7 0.004228 1

cuticular protein 144 (agap006369-pa)TC01631

1 0.003569 1

laccase-like multicopper oxidase 1 (TcLLP)TC01588

0 0 0

hexosaminidase isoform a (TcNAG1)TC00980

8 0 0

fused lobes (TcNAG3)TC00111

6 0 0

inwardly rectifying k+ (TcKir4)TC00670

8 0 0

aspartate 1-decarboxylase (TcADC2)TC01058

1 0 0

TcPMP6TC00850

6 0 0

peritrophic matrix protein 2-b (TcPMP7)TC00327

5 0 0

yellow-g-like proteinTC00622

6 0 0

protein yellow (Tcyellow-G2)TC00592

7 0 0

mucin-like protein (TcPMP3)TC00923

2 0 0

histidine decarboxylaseTC01006

2 0 0


0 0 0


7 0 0


8 0 0


8 0 0


9 0 0


7 0 0


2 0 0


3 0 0

cuticular protein 49aa cg30045-pbTC00454

7 0 0


8 0 0

cuticular protein 92fTC00664

6 0 0

cuticular protein rr-2 family (agap012466-pa) partialTC00698

9 0 0


0 0 0


1 0 0


7 0 0


0 0 0


9 0 0


0 0 0


3 0 0


7 0 0

cuticular protein analogous to peritrophins 1-iTC01276

6 0 0


7 0 0

cuticular protein 49abTC01313

3 0 0


7 0 0


8 0 0


9 0 0

chitinase 4TC00918

0 0 0

teratocyte released chitinase (chitinase 8)TC00962

4 0 0


5 0 0


6 0 0


7 0 0

chitinase 13 (chtinase 13 or 14?)TC00962

8 0 0


9 0 0


1 0 0


2 0 0


5 0 0


2 0 0


1 0 0


1 0 0


3 0 0


9 0 0


4 0 0

endocuticle structural glycoprotein bd-2TC00554

8 0 0


4 0 0


5 0 0

cuticle protein 6TC00840

0 0 0

cuticle protein 34TC01114

8 0 0

pupal cuticleTC01114

9 0 0


7 0 0


9 0 0

cuticular protein 49abTC01313

3 0 0


6 0 0


7 0 0


8 0 0


0 0 0


1 0 0


4 0 0


7 0 0


8 0 0


9 0 0


0 0 0


1 0 0


2 0 0


3 0 0


4 0 0


5 0 0


6 0 0


7 0 0


9 0 0


0 0 0


2 0 0


7 0 0

larval cuticle proteinTC01449

8 0 0


0 0 0


0 0 0


1 0 0

Table S3: Expression of immunity-related genes in the TcA cell line.

Gene name Gene family TC# RPKM Reads

PGRP-LD PGRPTC00254

6 0 0

PGRP-LA PGRPTC00278

9 3.933 585

PGRP-LC PGRPTC00279

0 2.398 394

PGRP-LE PGRPTC01050

8 0.491 69

PGRP-SA PGRPTC01061

1 2.053 174

PGRP-SB PGRPTC01362

0 0.1591 13

PGRP-LB PGRPTC01568

9 0.2557 23

βGRP1 βGRP/GNBPTC00229

5 0.0122 2


1 0.1679 35


9 0.1727 33

CTL1 C-type lectinTC00697

80.0425

7 6


40.0075

6 1


8 0.8264 144


7 0.7892 101


9 3.533 333


80.0324

2 3


3 0.1487 18


20.0074

6 1


8 0 0


50.0081

9 2


60.0170

6 15


2 3.676 1877


1 0.6879 69


1 0.9157 1438


7 5.207 394TC03075

4 0.8577 392


4 0.1236 46

GALE1 galectinTC00761

9 1.482 232


1 1.719 300


2 0.5352 286

FREP5 fibrinogen-likeTC00319

40.0048

6 1


6 0 0


7 0 0


8 0 0


4 0 0


40.0108

6 3


4 0 0

TEP-B TEPTC01466

4 4.961 3166

TEP-C TEPTC00966

70.0063

6 4

TEP-A TEPTC00937

5 3.081 2354

TEP-D TEPTC00080

80.0040

4 3

H1 cSPHTC00024

6 0.3804 62

H2 cSPHTC00024

7 10.41 1810

H3 cSPHTC00024

8 2.063 363

H4 cSPHTC00024

9 0.1466 27

H5 cSPHTC00025

0 0 0

H6 cSPHTC00025

2 1.253 639

P7 cSPTC00049

40.0120

8 2

P8 cSPTC00049

5 0.2597 42

P9 SPTC00049

6 0 0

P10 cSPTC00049

7 0 0

P11 SPTC00054

5 0 0

P12 SPTC00054

6 0 0

P13 SPTC00054

7 0 0

H14 SPHTC00054

80.0077

9 1

P15 SPTC00055

0 0 0

P16 SPTC00063

5 0.7388 92

H17 SPHTC00074

0 0.3815 48

H18 SPHTC00082

9 12.08 2063

P19 SPTC00087

00.0028

2 2

P20 SPTC03006

1 0 0

P21 SPTC03006

20.0253

2 3

P22 SPTC00102

3 0 0

P23 SPTC00115

7 0.0085 1

P24 SPTC03006

3 0 0

P25 SPTC03006

4 0 0

P26 SPTC03006

5 0 0

P27 SPTC00115

9 0 0

H28 cSPHTC00130

0 0 0

H29 cSPHTC00130

10.0389

8 6

H31 SPHTC00194

6 0.2612 34

P32 SPTC00206

1 0.1641 21

H33 cSPHTC00211

2 0 0

H34 cSPHTC00215

00.0147

3 2

H35 cSPHTC00219

30.0069

2 1

P36 SPTC00265

9 0.1779 16

P37 SPTC00276

6 0 0

P38 SPTC00276

7 0 0

P39 SPTC00276

8 0 0

P40 SPTC00278

5 0 0

P41 SPTC00278

6 0 0

P42 SPTC00308

10.0075

3 1

P43 SPTC00408

4 0 0

P44 cSPTC00416

0 1.674 367

P45 SPTC00441

8 0 0

P46 SPTC00452

3 0.4485 126.8

H47 SPHTC03006

6 0.3304 55

H49 SPHTC03006

8 0.5531 77

P50 SPTC00453

50.0798

6 22

H51 cSPHTC00462

20.0381

8 12

P52 cSPTC00462

4 0 0

P53 cSPTC00463

5 0.1205 26

P54 SPTC00465

40.0111

2 6

P55 cSPTC00477

0 3.733 602

P56 cSPTC00486

3 0.0065 1

H57 SPHTC00490

00.0126

7 2

P58 SPTC00493

7 0.7272 61

H59 cSPHTC00495

7 0 0

P60 cSPTC00513

00.0063

4 1

P61 cSPTC00523

00.0324

8 5

P62 SPTC00532

70.0485

4 14

H63 SPHTC00563

5 0 0

H64 SPHTC00590

8 0.1043 12

H65 SPHTC00592

5 0 0

P66 cSPTC00597

60.0307

6 5

P67 SPTC00602

60.0388

7 4

P68 SPTC00603

3 2.333 567

P69 SPTC00603

4 8.308 1717

H70 SPHTC00624

6 0.1742 18.98

H71 SPHTC00624

7 0.00910.998

9

P72 SPTC00626

80.0252

3 3

H73 SPHTC00626

9 0.0404 4

P74 SPTC00642

40.0160

1 2

P75 SPTC00643

80.0088

6 1

P76 SPTC00701

70.0089

3 1

P77 SPTC00701

9 0 0

H78 cSPHTC00702

6 0 0

P79 SPTC00826

7 0 0

P80 SPTC00850

4 0 0

H81 SPHTC00850

5 0 0

H82 cSPHTC00855

4 0 0

P83 cSPTC00865

30.0067

2 2

P84 cSPTC00865

7 0 0

H85 cSPHTC03060

9 0 0

P86 cSPTC03060

9 0 0

P87 cSPTC00865

9 0 0

H88 SPHTC00893

0 0 0

H89 SPHTC00893

1 0 0

P90 cSPTC00908

9 0.2052 33

P91 cSPTC00909

0 1.509 257

P92 cSPTC00909

1 0 0

P93 cSPTC00909

2 0.0123 2

P94 cSPTC00909

3 0 0

P95 cSPTC00909

40.0064

1 1

P96 SPTC00960

2 0 0

P98 SPTC03007

30.0057

3 1

H99 cSPHTC01007

6 0.8524 136

P100 SPTC01078

1 0 0

H101 SPHTC01090

4 0 0

H102 SPHTC03007

4 0 0

H103 SPHTC03007

5 0 0

H104 cSPHTC01090

6 0 0

H105 SPHTC01090

7 0 0

H106 SPHTC01090

8 0 0

H107 SPHTC01090

9 0 0

H108 SPHTC01091

0 0 0

H109 SPHTC01091

1 0 0

H110 SPHTC01092

7 0.0087 1

H111 SPHTC01092

9 0 0

H112 SPHTC01093

0 0 0

H113 SPHTC01093

2 0 0

H114 SPHTC01093

3 0 0

H115 SPHTC01093

4 0 0

H116 SPHTC01093

5 0 0

H117 SPHTC01093

6 0 0

H118 SPHTC01093

7 0 0

H119 SPHTC01093

8 0 0

H120 SPHTC01093

9 0 0

P121 SPTC01094

0 0 0

H122 SPHTC01094

10.0089

3 1

P123 SPTC01095

9 0 0

P124 SPTC01101

4 0.009 1

H125 cSPHTC01106

7 0 0

P126 cSPTC01107

8 0.2281 45

P127 SPTC01182

4 0 0

P128 SPTC01182

5 0 0

H129 SPHTC01239

0 0.2303 72

H130 SPHTC01257

30.0434

7 5

H131 SPHTC01257

40.0178

6 2

H132 SPHTC01257

5 1.45 173

P133 SPTC01304

2 0.0054 1

P134 SPTC01308

4 0 0

P135 SPTC01327

60.0078

1 1

P136 cSPTC01327

7 8.29 1380

H137 cSPHTC01327

80.0137

3 2

P138 cSPTC01327

9 0 0

P139 SPTC01328

0 2.039 268

P140 cSPTC01332

6 0.2445 37

P141 SPTC01341

5 0.6746 84

P142 cSPTC01341

60.0197

1 3

H143 SPHTC01342

1 0 0

P144 SPTC01361

30.0054

8 1

P145 SPTC01370

9 0 0

H146 SPHTC01389

4 3.797 3502

P147 SPTC01408

3 0 0

H148 SPHTC01437

5 0 0

P149 SPTC01439

1 0.0086 1

P150 SPTC01493

0 0 0

P151 SPTC01508

30.0576

1 7

H152 SPHTC01509

9 0 0

P153 SPTC01511

00.0087

5 3

P154 SPTC01513

00.0714

4 8

P155 SPTC01523

7 0 0

P156 SPTC01529

50.0198

6 7

P157 SPTC01529

70.0255

8 5

H158 SPHTC01534

4 0 0

H159 SPHTC01539

0 0 0

P160 SPTC01557

90.0433

1 5

P161 SPTC01558

00.0268

9 3

P162 SPTC01561

7 0 0

P163 SPTC01561

8 0 0

H164 cSPHTC01567

0 0.2239 67

P165 SPTC01577

9 0 0

P166 SPTC01578

00.0084

1 1

P167 SPTC01612

1 0.0094 1

P168 SPTC01637

2 0 0

serpin1 serpinTC00076

0 25.08 3861


50.0353

4 18


7 1.448 256


6 3.573 689


7 2.443 430


50.0650

3 16


0 3.966 679


10.0240

3 4


20.0089

3 1


3 0.0073 1


4 0 0


9 0 0


0 0 0


6 0 0


7 0 0


9 0 0


0 0.1517 25.97


1 0.3892 66.81


2 0.8893 151.9


3 0.4048 79.98


4 0.134 23


10.0239

7 4


5 0.1183 20


7 0 0


9 1.371 345


8 1.513 327


0 16.84 3669


9 1.576 304


7 4.132 720


40.0120

8 2

spz1 spätzleTC00052

0 1.359 134


30.0125

7 1


4 0.3429 41.96


8 1.001 135TC03063

9 0.7235 61


6 0 0


4 0 0


80.0112

3 2

Toll9 Toll-like receptorTC00062

5 0.1258 74


6 0.0116 3


8 2.467 1117


90.0740

7 27.99


20.0076

8 3


4 2.329 1320


50.0272

5 15


8 0.6483 340


1 0.1644 94

ML1 MD2-likeTC00820

2 1.707 107

ML2 MD2-likeTC00820

3 0 0

ML3 MD2-likeTC01406

80.0151

2 1

ML4 MD2-likeTC01406

90.0153

2 1

ML5 MD2-likeTC01635

1 1.135 79

ML6 MD2-likeTC00725

20.0292

8 2

ML7 MD2-likeTC01406

7 0 0

ML8 MD2-likeTC01635

2 0.1031 7

cactus cactusTC00200

3 2.173 342

pelle pelleTC01536

5 0.2709 52

Myd88 Myd88TC00318

5 1.009 175

Tube TubeTC01189

5 0.6931 193

pellino pellinoTC00967

2 1.966 397

Traf2 TrafTC00770

6 1.038 179

cactin cactinTC00878

2 0.7151 329

Dif1 RELTC00769

7 2.375 728

Dif2 RELTC00809

6 0.9852 164

FADD FADDTC01404

2 0.3658 31

IKKb IKKbTC00141

9 0.2231 67.99

IKKb IKKbTC00979

8 1.745 553

IKKg IKKgTC00054

1 1.276 278

IMD IMDTC01085

1 0.6013 52

TAK1 TAKTC00557

2 0.5737 127

casps1 caspaseTC00384

1 0.3954 53


1 0.338 57


0 0.7709 132

casps4 dredd/casp8TC01402

6 0.756 186


50.0072

7 1


9 0.1836 32


7 0.6385 39.75


8 0 0

caspar blocking caspaseTC00998

5 0.6829 194

IAP2 IAPTC00118

9 1.934 414

IAP1 IAPTC00119

2 4.837 709

IAP3 IAPTC00984

8 1.629 3054

IAP4 IAPTC00270

9 0.5984 37

REL1 RELTC01119

1 2.687 992

REL2 RELTC01470

8 2.489 1129

Tab2 Tab2TC00595

2 0.4862 111

Hep HepTC00038

5 0.4439 124

basket1 basketTC00681

0 0.7394 125


7 0.4588 74


4 2.064 315

Jra JraTC00681

4 2.353 232

kay kayTC01187

0 6.365 1010

DOME DOMETC00187

4 3.056 1455

HOP HOPTC00864

8 0.5002 109

STAT STATTC01321

8 3.5 1182

proPO1 prophenoloxidaseTC00032

50.0135

4 4


70.0135

1 3.994


80.0416

3 5.994

MI melanization inhibitorTC00634

20.0065

2 1

hexamerin1 hexamerinTC00537

4 0 0


5 0 0


6 0 0


7 0 0


5 0.1873 57


9 0 0

catalase1 catalaseTC01138

5 0 0

catalase2 catalaseTC01109

00.0096

4 2

GTX1 glutathione oxidaseTC01036

2 1.156 100


5 10.52 769


4 0.3835 33

HPX1 heme peroxidaseTC00549

3 0.6148 390


4 0 0


20.0818

7 32


9 0.1915 64


1 2.905 932


1 0 0


50.0377

5 11


10.0497

8 13


6 0.333 200


8 0.2029 133


20.0048

6 2

TPX6 peroxiredoxinTC01492

9 10.3 877


00.0469

6 4


8 0.7215 73


1 1.839 194


1 0.3259 31


80.0206

5 2

SOD3 superoxide dismutaseTC00701

1 11.67 777


6 48.72 3518


5 2.932 289


0 0.5553 267

attacin1 antimicrobial peptideTC00773

7 0.1115 8


8 0.1267 8


90.0155

2 1cecropin1 antimicrobial peptide

cecropin2 antimicrobial peptideTC03048

2 0 0

cecropin3 antimicrobial peptideTC00050

00.0254

2 1

defensin1 antimicrobial peptideTC00625

00.0347

6 1.999defensin2 antimicrobial peptide TC01051 0.0578 2

7 2

defensin3 antimicrobial peptideTC01246

9 3.634 132defensin4 antimicrobial peptidecoleoptericin1 antimicrobial peptide

TC005093 0 0

coleoptericin2 antimicrobial peptide

TC005096

0.01626

0.9986

lysozyme1 lysozymeTC01034

90.0083

8 1


0 0 0


1 0 0


2 0 0

WAP antimicrobial peptideTC01132

40.0752

1 4

neuroglian neuroglian/hemolinTC00188

9 7.538 4133

SR-B6 scavenger receptorTC00094

80.0174

5 4


7 2.442 490

SR-A1 scavenger receptorTC00786

1 1.826 2364


10.0044

6 1


90.0538

9 12


00.0606

5 37


8 0 0


3 0 0


6 0.2123 113.9


30.0383

9 8


6 0 0


7 0 0


8 0 0


4 3.797 3502


6 0.1704 42


1 0.1133 27


4 5.154 1150


00.0087

5 3


40.0464

2 11

SR-C scavenger receptorTC01564

00.0807

8 19


40.0041

7 1

NimA nimrodTC01142

70.0155

6 3

NimB nimrodTC01142

8 8.335 1283

NimCl1 nimrodTC00205

3 0 0

NimCl2 nimrodTC01525

8 0 0

draper draperTC00068

9 2.257 970

Figure S1.

The map for the plasmid used in this study. The sequence is deposited in NCBI with the GenBank Accession ############.

· Web view1Department of Anatomy and Physiology, Kansas State University, Manhattan, KS 66506. 2Department of Entomology, Kansas State University, Manhattan, KS 66506. 3USDA, Agricultural

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