„Illuminating the Evolutionary History of Chlamydiae” (Horn et al.) Supporting Online Material 1 Materials and methods Genome sequencing UWE25 was cultivated in Acanthamoeba sp. UWC1 (1). Elementary bodies were harvested and purified by step gradient density centrifugation. High molecular weight genomic DNA was isolated from purified elementary bodies by proteinase K/SDS incubation and guanidinium-isothiocyanate/isobutanol precipitation on silica. Whole-genome shotgun libraries were obtained by fragmenting genomic DNA using mechanical shearing and cloning 1.0 - 3.0 kilobase (Kb) fragments into pGEM-T vectors (Promega, Madison, WI, USA). Double-ended plasmid sequencing reactions were carried out using BigDye Terminator chemistry on ABI 3700 capillary sequencers (Applied Biosystems, Foster City, CA). The whole genome sequence of UWE25 was assembled from 27,353 reads with an 8.8-fold redundancy using the Paracel Genome Assembler software (Paracel Inc., Pasadena, CA) and the Staden Package (http://www.mrc-lmb.cam.ac.uk/pubseq/staden_home.html). Gap closure was performed by Vectorette PCR (2), combinatorial PCR, and primer walking. Sequencing or sub-cloning of a single approximately 5 kb PCR fragment, which would have been required to close the genome sequence, was not possible despite of major efforts in two independent laboratories (MWG Biotech and University of Vienna). Genome analysis The PEDANT software system was used for genome sequence analysis (3) and annotation (http://pedant.gsf.de). Prediction of coding sequences (CDSs) was performed with Orpheus (4). Translated CDSs longer than 180 nucleotides were searched against a non-redundant
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„Illuminating the Evolutionary History of Chlamydiae” (Horn et al.)
Supporting Online Material
1
Materials and methods
Genome sequencing
UWE25 was cultivated in Acanthamoeba sp. UWC1 (1). Elementary bodies were harvested
and purified by step gradient density centrifugation. High molecular weight genomic DNA
was isolated from purified elementary bodies by proteinase K/SDS incubation and
guanidinium-isothiocyanate/isobutanol precipitation on silica. Whole-genome shotgun
libraries were obtained by fragmenting genomic DNA using mechanical shearing and
cloning 1.0 - 3.0 kilobase (Kb) fragments into pGEM-T vectors (Promega, Madison, WI,
USA). Double-ended plasmid sequencing reactions were carried out using BigDye
Terminator chemistry on ABI 3700 capillary sequencers (Applied Biosystems, Foster City,
CA). The whole genome sequence of UWE25 was assembled from 27,353 reads with an
8.8-fold redundancy using the Paracel Genome Assembler software (Paracel Inc., Pasadena,
CA) and the Staden Package (http://www.mrc-lmb.cam.ac.uk/pubseq/staden_home.html).
Gap closure was performed by Vectorette PCR (2), combinatorial PCR, and primer
walking. Sequencing or sub-cloning of a single approximately 5 kb PCR fragment, which
would have been required to close the genome sequence, was not possible despite of major
efforts in two independent laboratories (MWG Biotech and University of Vienna).
Genome analysis
The PEDANT software system was used for genome sequence analysis (3) and annotation
(http://pedant.gsf.de). Prediction of coding sequences (CDSs) was performed with Orpheus
(4). Translated CDSs longer than 180 nucleotides were searched against a non-redundant
„Illuminating the Evolutionary History of Chlamydiae” (Horn et al.)
Supporting Online Material
2
protein sequence database derived from PIR (5), SwissProt (6) and trEMBL using blastp
(cut-off p-value <=1e-04) (7) and further analyzed by searching against Pfam (8), Prosite,
COGs (9), PIR, and BLOCKS (10). Structural predictions of derived proteins were carried
out by the IMPALA software searching against SCOP (11) and PDB, and additional tools
implemented in PEDANT. The comprehensive data collected automatically for each CDS
by PEDANT were subsequently used for manual annotation, which included additional
FastA searches against SwissProt and assignment of CDSs to functional categories
according to PEDANT’s functional catalogue FunCat (3). Only CDSs with amino acid
sequence homology to characterized proteins (either by knock-out/complementation
experiments, protein expression, or by known 3D structure) were annotated as “strongly
similar to known proteins” (> 40% amino acid sequence identity to characterized protein)
or as “similar to known proteins” (> 20% amino acid sequence identity to characterized
protein). CDSs with sequence homology to not yet functionally characterized proteins were
classified as “conserved hypothetical proteins” (> 30% amino acid sequence identity to first
blast hit) or “hypothetical proteins” (> 20% amino acid sequence identity to first blast hit).
CDSs showing no significant homology to proteins in public databases were annotated as
“unknown protein”. Biochemical pathway prediction and reconstruction was performed
using KEGG (12). tRNA genes were identified with the tRNAscan-SE program (13).
Prediction of subcellular location of plant proteins was performed using TargetP (14),
iPSORT (15), and ChloroP (16). The origin of replication was determined by cumulative
GC-skew analysis using GenSkew (fig. S8; http://mips.gsf.de/services/analysis/genskew).
Codon usage and codon adaptation index were calculated using the programs cusp and cai
„Illuminating the Evolutionary History of Chlamydiae” (Horn et al.)
Supporting Online Material
3
included in the EMBOSS software package (17). Comparative genome sequence analysis
with Chlamydophila pneumoniae CWL029, Chlamydophila caviae, Chlamydia trachomatis
sv D and Chlamydia muridarum were performed using SIMAP, the mips Similarity Matrix
of Proteins (http://mips.gsf.de/services/analysis/simap). A linear representation of the
UWE25 genome is available as fig. S9.
Phylogenetic sequence analysis
The software ARB (18) was used for phylogenetic sequence analysis of selected proteins
(http://www.arb-home.de). Protein databases were constructed by blastp search (7) against
147 complete microbial and eukaryotic genome sequences. Amino acid sequences were
aligned automatically with ClustalW (19) implemented in ARB and the resulting alignment
was refined manually. Phylogenetic amino acid sequence trees were constructed by
applying neighbor joining (PAM and Kimura correction), PHYLIP distance matrix (Fitch),
PHYLIP maximum parsimony methods (20), and maximum likelihood approaches using
PROTml 2.3 (JTT amino-acid replacement model) and TREE-PUZZLE (21) implemented
in ARB. Maximum parsimony bootstrap values (1000 resamplings) and TREE-PUZZLE
support values were calculated to estimate the reliability of obtained tree topologies.
Data deposition
In addition to the genome sequence data deposited at EMBL/GenBank/DDBJ (accession
number BX908798), the comprehensive UWE25 genome database is available at
http://mips.gsf.de/services/genomes/uwe25.
„Illuminating the Evolutionary History of Chlamydiae” (Horn et al.)
Supporting Online Material
4
Metabolism of UWE25
Glycolysis
Pathogenic chlamydiae lack a glucokinase and have an incomplete phosphotransferase
system (PTS), and therefore rely on import of phosphorylated sugars for glycolysis (22,
23). The UWE25 genome also lacks key components of the PTS and encodes a glucose-6-
phosphate transporter (pc0387) orthologous to the respective transporters in pathogenic
chlamydiae. In addition, and unlike its pathogenic counterparts, UWE25 possesses a
glucokinase (pc0935) and does thus not depend on host-derived phosphorylated sugars. The
presence of a pyrophosphate-dependent phospofructokinase (pc0880) indicates that
UWE25 like pathogenic chlamydiae is able to generate four ATP molecules during
glycolysis.
Pentose phosphate pathway
The pentose phosphate pathway of UWE25 is complete. Like pathogenic chlamydiae (23),
UWE25 is thus able to generate pentose phosphates, erythrose-4-phospate and to reduce
nicotinamide adenine dinucleotide phosphate (NADP) independently from its host.
Oxidative phosphorylation
Both, UWE25 and pathogenic chlamydiae have a respiratory chain most similar to the
respiratory chain of Escherichia coli expressed under microaerophilic conditions (22, 23).
In addition to a Na+-dependent NADH oxidoreductase (pc0559-pc0572), a cytochrome bd
complex (pc1629, pc1630), and a V-type ATPase (pc1676-pc1680, pc1682) present in
„Illuminating the Evolutionary History of Chlamydiae” (Horn et al.)
Supporting Online Material
5
pathogenic chlamydiae, UWE25 also encodes a H+-translocating NADH oxidoreductase
(pc0559-pc0572), a cytochrome C (pc0909), a cytochrome C oxidase (pc1189-pc1193), and
an additional F-type ATPase (pc1667-pc1674).
Amino acids, nucleotides, and cofactors
Amino acid biosynthesis pathways in both, UWE25 and pathogenic chlamydiae, are
frequently truncated (23). Like pathogenic chlamydiae, UWE25 is not able to generate
threonine, cysteine, valine, leucine, isoleucine, lysine, arginine, phenylalanine, and
tyrosine. Surprisingly UWE25 also lacks most key enzymes of the tryptophan biosynthesis
pathway, which is complete in some pathogenic chlamydiae (24-29). This clearly contrasts
with the generally greater metabolic/biosynthetic capabilities of UWE25. UWE25 probably
does not require to synthesize tryptophan because it should not experience tryptophan
depletion in its host, which is observed as immune response in mammalian cells upon
infection with pathogenic chlamydiae. In contrast to pathogenic chlamydiae, however,
UWE25 is able to generate glycine, serine, glutamine, and proline. Purine and pyrimidine
biosynthesis pathways are equally incomplete in UWE25 as in pathogenic chlamydiae (23),
which thus rely on the import of nucleotides from their host cells. Both, pathogenic
chlamydiae and UWE25 lack key enzymes of several cofactor biosynthesis pathways (23)
and thus have to acquire cofactors like coenzymeA and nicotinamide adenine dinucleotide
(NAD) from their hosts. However, in contrast to pathogenic chlamydiae, UWE25 is able to
produce menaquinone, which can be used instead of host-derived ubiquinone in the
quinone pool of the respiratory chain.
Illuminating the evolutionary history of chlamydiae (Horn et al.)
million years ago
2000
1500
present
700
hominids*
environmental – pathogenic chlamydiae+
protist – plant/animal/fungi#
rickettsial ancestor– mitochondria#
chlamydiae – rickettsiae+
2200 cyanobacteria – Gram+/Gram- bacteria#
1200 plant – animal#
250 C. trachomatis – C. pneumoniae+
Fig. S1 Evolutionary time scale of chlamydiae. Estimates of major divergence events are based on 16S rRNAdissimilarities and an assumed divergence rate of 1% per 50 million years (30) (+), or taken from Hacia et al. (31) (*), and Feng et al. (32) (#), respectively.
Illuminating the evolutionary history of chlamydiae (Horn et al.)
711
7 7
16 11 Ctr
Cpn
Cmur
Cca
Chlamydiaceae homologues total: 938
6
11
14
527
18
39
4
19
43
1093
Fig. S2 Number of UWE25 proteins with sequence homology (fastA score/selfscore > 0.1) to proteins of pathogenic chlamydiae. Chlamydophila pneumoniae CWL029 (Cpn), Chlamydophila caviae (Cca), Chlamydia trachomatis sv D (Ctr), Chlamydia muridarum (Cmur).
Illuminating the evolutionary history of chlamydiae (Horn et al.)
UW
E25
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omat
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urid
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n
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Fig.
S3
Com
paris
on o
f gen
ome
synt
eny
betw
een
UW
E25
and
path
ogen
ic c
hlam
ydia
eba
sed
on r
ecip
roca
l fa
stA
best
mat
ches
(sc
ore/
selfs
core
> 0.
2).
(A)
UW
E25
ver
sus
C.
trach
omat
issv
D;
(B)
UW
E25
ver
sus
C.
mur
idar
umM
oPn;
(C
) U
WE
25 v
ersu
s C
. pn
eum
onia
eC
WL0
29;
(D)
UW
E25
ver
sus
C.
cavi
aeG
PIC
. G
enom
es h
ave
been
ro
tate
d so
that
the
repl
icat
ion
orig
in is
at 0
kb.
Illum
inat
ing
the
evol
utio
nary
hist
ory
of c
hlam
ydia
e (H
orn
et a
l.)
Illuminating the evolutionary history of chlamydiae (Horn et al.)
Fig. S4
Illuminating the evolutionary history of chlamydiae (Horn et al.)
Fig. S4Circular representation of the UWE25 genome showing predicted coding regions and other features. Outer two circles, predicted CDSs on the plus and minus strand, respectively, classified by biological role: amino acid metabolism (light orange), cellular processes (cyan), nucleotide metabolism (dark green), lipid/fatty acid metabolism (salmon), metabolism of vitamins/cofactors (purple), energy metabolism (light green), cell cycle/DNA processing (purple), transcription (yellow), protein synthesis and fate (chartreuse), transport (dark blue), detoxification and stress response (magenta), virulence and defense (brown), others (lemon green), cell envelope (olive), mobile elements (red), leu-rich repeat proteins (orange), (conserved) hypothetical proteins(sea green), unknown proteins (light blue), rRNA and tRNA genes (medium blue), pseudogenes (grey). Third and fourth circle, taxonomic classification of first BLAST hit for predicted CDSs on the plus and minus strand, respectively: Chlamydiaceae (orange), Proteobacteria (light blue), Firmicutes (red), Cyanobacteria (dark blue), Actinobacteria (yellow), Archaea (light green), other prokaryotes (purple), fungi (light pink), plants (dark green), mammals (pink), other animals (brown). Fifth circle, CDSs with homology to proteins of pathogenic chlamydiae (reciprocal best hit, fastA score/selfscore > 0.1). Sixth circle, (remnants of) mobile genetic elements. Seventh circle, rRNA (light blue) and tRNA(dark blue) genes. Eighth circle, GC-skew.
Illuminating the evolutionary history of chlamydiae (Horn et al.)
37%
22%
12%
6%
5%
3%
2%
2%
1%
1%9%
ChlamydiaeProteobacteriaFirmicutesCyanobacteriaPlantsArchaeaMammalsActinobacteriaFungiother animalsother prokaryotes
Fig. S5Distribution of taxonomic classification of first BLAST hits of predicted UWE25 CDSs.
Illuminating the evolutionary history of chlamydiae (Horn et al.)
Fig. S6Phylogeny of four selected UWE25 proteins related to plant proteins. Distance matrix trees (Fitch (20)) are shown. Maximum parsimony bootstrap values and TREE-PUZZLE support values are indicated at each node of the tree. Bar, 10% estimated evolutionary distance.
AspC
FabI
PgmA
RibA
Chlamydia trachomatisChlamydia muridarum
Chlamydophila caviaeChlamydophila pneumoniae
UWE25Oryza sativa
Arabidopsis thalianaGeobacter metallireducens
Bacteroides thetaiotaomicronSynechococcus sp.Prochlorococcus marinus
Nostoc sp.Thermosynechococcus elongatus
Chlamydia trachomatisChlamydia muridarum
Chlamydophila caviaeChlamydophila pneumoniae
UWE25Oryza sativa
Arabidopsis thalianaGeobacter metallireducens
Bacteroides thetaiotaomicronSynechococcus sp.Prochlorococcus marinus
Nostoc sp.Thermosynechococcus elongatus
Thermosynechococcus elongatusProchlorococcus marinus
Synechococcus sp.Nostoc sp.
Campylobacter jejuniHelicobacter pylori
Sinorhizobium melilotiAgrobacterium tumefaciens
Chlamydophila caviaeChlamydia muridarum
Chlamydia trachomatisChlamydophila pneumoniae
UWE25Petunia hybridaOlea europaea
Nicotiana tabacumArabidopsis thalianaOryza sativa
Thermosynechococcus elongatusProchlorococcus marinus
Synechococcus sp.Nostoc sp.
Campylobacter jejuniHelicobacter pylori
Sinorhizobium melilotiAgrobacterium tumefaciens
Chlamydophila caviaeChlamydia muridarum
Chlamydia trachomatisChlamydophila pneumoniae
UWE25Petunia hybridaOlea europaea
Nicotiana tabacumArabidopsis thalianaOryza sativa
Chlamydophila pneumoniaeChlamydophila caviae
Chlamydia muridarumChlamydia trachomatisUWE25Arabidopsis thaliana
Gloeobacter violaceusProchlorococcus marinusSynechococcus sp.
Chlamydophila pneumoniaeChlamydophila caviae
Chlamydia muridarumChlamydia trachomatisUWE25Arabidopsis thaliana
Gloeobacter violaceusProchlorococcus marinusSynechococcus sp.
Prochlorococcus marinusSynechococcus sp.Prochlorococcus marinus
Leptospira interrogansCytophaga hutchinsonii
Aquifex aeolicusDesulfitobacterium hafniense
Corynebacterium glutamicumMycobacterium avium
Arabidopsis thalianaLycopersicon esculentum
Oryza sativaUWE25
Chlamydia trachomatisChlamydia muridarum
Chlamydophila pneumoniaeChlamydophila caviae
100/81
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Illuminating the evolutionary history of chlamydiae (Horn et al.)
Fig. S7 Phylogeny of the chlamydial tricarboxylic acid (TCA) cycle. Unrooted consensus tree based on a concatenated amino acid sequence alignment comprising FumC (fumarate hydratase), Mdh (malatedehydrogenase), SucB (2-oxoglutarate dehydrogenase) and SucC (succinate-CoA ligase). The monophyletic grouping of pathogenic and environmental chlamydiae (to the exclusion of all other bacteria) demonstrates the origin of these TCA cycle proteins from the last common chlamydial ancestor. Polytomicbranching points indicate those parts of the tree for which different treeing methods produced different topologies. Maximum parsimony bootstrap and TREE-PUZZLE support values are indicated at each node of the tree. Bar, 10% estimated evolutionary distance.
Bradyrhizobium japonicum USD10Brucella melitensis 16M
Caulobacter crescentus CB15Rickettsia prowazekii
Pirellula sp. 1Bacillus subtilis subsp. subtilis 168
Staphylococcus aureus subsp. aureus Mu50. Escherichia coli CFT073Haemophilus influenzae Rd KW20
Vibrio cholerae O1 biovar eltor N16961Coxiella burnetii RSA 493Leptospira interrogans sv Lai 56601
Anopheles gambiae PESTUWE25
Chlamydia muridarum MoPnChlamydia trachomatis sv DChlamydophila caviae GPICChlamydophila pneumoniae CWL029
Nitrosomonas europaea ATCC19718Bordetella pertussis Tohama IRalstonia solanacearum
Xanthomonas campestris pv. campestris ATCC33913Xylella fastidiosa 9a5cChromobacterium violaceum ATCC12472
Deinococcusradiodurans R1Corynebacterium glutamicum ATCC13032
Mycobacterium tuberculosis H37Rv
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Illuminating the evolutionary history of chlamydiae (Horn et al.)
Fig. S8GC-skew analysis of the UWE25 genome sequence. Blue graph, GC-skew; red graph, cumulative GC-skew; red line, predicted oriC.
0 10000 20000 30000 40000 50000 60000
pc0001pc0005
pc0006pc0007
pc0012
glmUrfaF
nth
thdFahpC
pc0025
tRNA-Argpc0026
pc0029
groELgroES
pc0032
pc0036pc0038
pc0002pc0003
pc0004recC
recB
recDpc0011
ssuA
ssuBssuC
pc0018
ruvCruvA
psdD
prmApc0028
putA
uvrDpc0035
pc0037
pc0039
70000 80000 90000 100000 110000 120000
pc0040
pc0041pc0042
pc0043
pc0044pc0045
pc0046
pc0048pc0049
pc0050
pc0051pc0052
pc0053
pc0054pc0055
pc0056
pc0057pc0058
pc0059
pc0060pc0061
pc0062
pc0063pc0064
pc0047
130000 140000 150000 160000 170000 180000
pc0065
pc0066pc0067
pc0068
aspCpc0070
topA
dprAaroB
pc0075
pc0076pc0082
gpsA
pc0084pc0085
pc0086
bcppc0093
oppF
oppDoppC
oppB
oppApc0104
pc0105
pc0107fliY
nrdD
pc0112pc0113
batE
pc0115pc0116
tRNA-Ala
pc0074parB
copA
pc0079pc0080
pc0081
pc0087pc0088
mutY
pc0090hemB
napA
nqrApc0098
pc0099
pc0100glgP
glgC
pc0110
190000 200000 210000 220000 230000 240000
pc0117
pc0118batA
pc0121
emrBemrA
pc0129
pc0130rluA
mutL
pc0145pc0147
aspH
pc0150pc0155
pc0158
pc0159yjbC
pc0164
birA
cicApc0122
rfbArfbC
rfbD
rfbBrpsT
pc0133
pc0134pc0135
tRNA-Gly
rpsBtsf
pc0138
pcnBpc0141
pc0142
enopc0144
secA
arsABlpdA
lipA
pc0153pc0154
pc0156
pc0157pgmA
nifS
pc0163rodA
250000 260000 270000 280000 290000 300000
pc0175dinG
rpoDpc0180
wzt
wzmpc0183
pc0185
pc0194npr
glpQ
pc0197sctT
sctS
sctRsctL
pc0204
sctJrs10
fusA
rpsGrs12
pc0210
pc0211pc0212
pc0167
tyrPtyrP
trpS
pc0171pc0172
pc0173
uvrBpc0178
pc0179
pc0184wza
wzc
pc0188sufB
sufC
sufDsufS
pc0193
ppaBppaA
pc0213
310000 320000 330000 340000 350000 360000
pc0215
pc0216pc0220
rho
yacEpolA
sohB
ipsFargS
pgsA
pc0243gltX
pc0245
tsppc0248
recJ
pc0252pc0253
tsptRNA-Phe
rplU
rl27obg
pc0225
cysJpc0228
murA
kamAclpB
pc0233
yjeEpc0235
dnaQ_2
pc0237hisH
pgk
ntt_3ntt_2
euo
ntt_1secDF
pc0254
pc0255ytgA
ytgB
ytgCytgD
dxr
pc0261
370000 380000 390000 400000 410000 420000 430000
pc0264
ptsNptsN
dut
accDsodM
pc0271
hemCsms
rnc
pc0277pc0285
cadA
czcAczcB
czcC
pc0292pc0293
pc0294
pc0295pc0296
pc0297
nqrEnqrD
nqrC
nqrBpc0302
corA
dnaApc0309
pc0310
pc0262pc0263
pc0265pc0275
pc0276
pc0278pc0279
pc0280
gcvTgcvH
gcvP1
gcvP2alkB
pc0291
pc0303pc0304
comF
pc0308mraW
pc0312
440000 450000 460000 470000 480000 490000
tRNA-ArgplsC
cmk
cdsAuppS
lepA
tRNA-Leupc0324
pc0329
pc0333ppaA
ppaA
ppapc0337
pc0338
pc0339tRNA-Arg
pc0342
pc0343pc0344
hemA
pc0349pc0358
tRNA-Arg
pc0359pc0360
pbp2murE
amiA
pc0316pgd
pc0323
pc0325yaeI
ispD
truApc0330
tRNA-Leu
pc0331pc0332
pc0340
tRNA-Leupc0341
nfo
asnSpc0348
gyrB
gyrApc0352
pc0353
pc0354rluD
dtd
pc0357folK
kdsA
pc0363pc0364
pc0365
500000 510000 520000 530000 540000 550000
ndk
pabAndk
fdxC
pc0372rapA
pc0378
pc0380pc0382
mip
aspShisS
pc0386
pc0393ksgA
pc0396
fmupc0405
pc0406
pc0407
pheTpc0370
pc0373
pc0374pc0376
pc0377
trxAkefC
uhpC
pc0388dnaE
pc0390
pc0391rsbW
dac
ddlAzip
cutE
envAfabZ
lpxA
fmtpc0408
pc0409
pc0410pc0411
rplC
rplDrplW
rplB
rpsSrplV
rpsC
rplPrpmC
560000 570000 580000 590000 600000 610000
pc0436pc0438
pc0441dapF
clpP
glyApc0445
pc0447
pc0448folE
trmH
pc0451pc0452
metQ
metIabc
pc0456
pc0457xerC
elaC
glpGrfaE
lon
pc0469ftsK
bacA
tRNA-Hispc0472
pc0477
rpsQ
rplNrplX
rplE
rpsHrplF
rplR
rpsErplO
secY
rpsMrpsK
rpoA
rplQgapA
pc0437
tRNA-Leupc0439
pc0440
pc0446pc0463
pc0464
dgtpc0466
rpsU
dnaJpc0473
ptsK
ptsHptsI
Illuminating the evolutionary history of chlamydiae (Horn et al.) Fig. S9 (1/4)
620000 630000 640000 650000 660000 670000
dnaXpc0480
pc0481
pc0482pc0483
pc0488
pc0489pc0490
pc0492
lepBpc0497
pc0499
pc0502pc0511
pc0512
pc0525pc0527
pc0530
pc0532pc0533
pc0539
pc0540def
pc0479
phoHntt_4
pc0486
mutTpc0491
pc0493
ileSpc0495
pc0498
pc0500pc0501
tRNA-Met
alkApc0504
pc0505
pc0506pc0507
pc0508
pc0509pc0510
pc0513
pc0514pc0515
ps-gene
pc0516pc0517
ada
satGpc0520
pc0521
pc0522pc0523
pc0524
pc0526pc0528
pc0529
cptpc0534
pc0535
pc0536pc0537
rtxA
pc0542mocA
680000 690000 700000 710000 720000 730000
nasA
pc0547prfB
pc0553
pc0554rfbB
pc0556
pc0558pc0573
pc0577
pc0579pc0581
nasD
pc0592pc0593
pc0545
mappc0549
acrA
acrBoprK
pc0557
nuoAnuoB
nuoC
nuoDnuoE
nuoF
nuoGnuoH
nuoI
nuoJnuoK
nuoL
nuoMnuoN
pc0574
pc0575pc0576
pc0578
pc0580pc0582
PCKI 1
pc0584pc0585
pc0586
pc0587tyrP
pc0590
dnaKinfA
tRNA-Thr
tufAtRNA-Trp
pc0596
secEps-gene
rplK
rplArplJ
rplL
740000 750000 760000 770000 780000 790000
pc0607
pc0609mafpc0611
pc0615omcB
omcA
pc0618dxs
xseB
xseAnfipc0624
pc0628tRNA-Ser
pc0630
alracrD
pc0634
tolCpc0636
pc0637
pc0641cps
pnp
rpsOpc0645
pc0646
pc0650
rpoBrpoC
pal
pc0608pc0612
pc0613
pc0614dagA
pc0625
pc0626pc0627
pc0629
pc0632pc0638
pc0639
ftsHpc0647
pc0648
pc0649rpmE
prfA
800000 810000 820000 830000 840000 850000 860000
pc0661rpmB
pc0667pc0668
gatB
gatAgatC
pc0674
pc0675recN
pc0678
rnhBtruA
pc0683
pc0684aspC
dapA
dapBpc0688
recD
glySpc0694
pc0698
pc0699pc0700
pc0708
hemKffh
rpsP
trmDrplS
rnh
pc0659pc0660
gmk
pc0663pc0664
metG
phrBpc0673
pc0676
pc0680pc0681
dnaG
pc0691pc0692
pc0695
pc0696uvrC
tRNA-Val
thrSinfC
rpmI
rplTpheS
pc0706
rfaC
870000 880000 890000 900000 910000 920000
pc0709pc0710
pc0711
pc0712pc0713
pc0714
sugEpc0716
pc0722
pc0723clpB
pc0729
pc0733pc0734
pc0736
pc0738rhs
pc0741
pc0742pc0744
malQ
pc0717pc0718
pc0719pc0720
nifS
tRNA-Leupc0724
udhA
pc0726pc0727
pc0730
pc0731pc0732
pc0735
pc0737gcpE
pc0743
ps-gene
930000 940000 950000 960000 970000 980000
sycEpc0747
pc0748
sctVsctU
pc0755
pc0756pc0757
ribF
truBrbfA
pc0761
nusApc0763
rpsA
braBpc0775
pc0776
tRNA-AsptRNA-Val
map1
pc0784pc0785
pc0786
pc0790pc0791
pc0792
mscLpc0794
pc0795
pc0800def
pc0751pc0752
pc0753pc0754
lysC
pc0766rpe
efp
pc0770accB
accC
pc0773pc0774
pc0777
exoDpc0779
pgi
pc0782xerD
lcrH
pc0788adh3
pc0796
pc0797pc0798
pc0799
tpiAsecG
ps-gene
990000 1000000 1010000 1020000 1030000 1040000
pc0807
pc0808tdh
pc0811
pc0812lolD
pc0816
rpmGpc0818
pgl
pc0820zwf
pc0823
pyrGkdsB
pc0829
pc0831pc0832
pc0833
pc0847pc0848
pc0850
pc0854pc0855
tRNA-Tyr
tRNA-Thrpc0859
pc0863
pc0804pc0805
pc0806
copBpc0813
pc0814
pc0822pc0826
kdtA
tRNA-MettRNA-Met
pc0828
pc0830pc0834
pc0835
pc0836pc0837
pc0838
pc0839pc0840
pc0841
pc0842pc0843
pc0844
pc0845pc0846
pc0849
pc0851pc0852
pc0853
pcmpc0857
pc0858
pc0860pc0861
pc0862
1050000 1060000 1070000 1080000 1090000 1100000
pc0866pc0867
pc0870
pc0875pc0876
ribE
ribAribC
mutM
pc0894pc0895
pc0896
rbnpc0899
pc0902
pc0903pc0905
pc0907
pc0908pc0909
pc0912
pc091
surEacnB
pc0868
pc0869potA
potB
potCpotD
mgtE
sdaBpc0879
pfkA
pc0881aroA
aroL
aroCpc0885
pc0886
pc0887pc0888
rbp
pc0897pc0900
pc0901
rgpBpc0906
pc0910
pc0911
1110000 1120000 1130000 1140000 1150000 1160000
czcDkefC
pc0916pc0917
pc0918
pc0919pc0924
pc0925
pc0926pc0927
trpD
cynTpc0931
pc0932
dhnApc0934
glk
pc0936pc0937
pc0938
pc0940pc0942
pc0944
pc0945pc0946
trxA
pc0948pc0949
lig
pc0951pc0952
yciF
pc0954pc0957
dcd
pc0960pc0963
msrA
pc0966pc0967
pc0968
pc0969pc0970
pc0920pc0921
pc0922pc0923
pc0929
pc0939pc0941
blc
ftnpc0956
pc0959
ps-genepc0961
pc0962
pc0965
1170000 1180000 1190000 1200000 1210000 1220000
pc0972
pc0973pc0974
umuC
umuDpc0977
pc0978
pc0979pc0981
pc0984
pc0994pc0995
pc0996
pc0997pc0999
pc1000
pc1001pc1006
pc1007
pc1008pc1009
pc1010
pc1011pc1012
doc
pc1022pc1023
pc1026
pc1030pc1031
pc1032
pc1033pc1034
parA
pc10375S-rRNA
23S-rRNA
pc0971pc0980
pc0982pc0983
pc0985
pc0986pc0987
pc0988
pc0989pc0990
pc0991
pc0992pc0993
pc0998
pc1002pc1003
pc1004
pc1005pc1013
pc1014
ps-genepc1015
pc1016
pc1017pc1018
pc1019
pc1020pc1024
pc1025
pc1027pc1028
pc1029
pc1036
Illuminating the evolutionary history of chlamydiae (Horn et al.) Fig. S9 (2/4)
1230000 1240000 1250000 1260000 1270000 1280000 1290000
16S-rRNApc1038
pc1039
adkpc1043
pc1044
pc1045leuS
pc1052
cynTmenE
menA
menBpc1067
menD
menFpc1070
pc1071
holBtmk
gyrA
gyrBpc1076
pc1077
lytBwzb
pc1040ribD
pc1046vacB
pc1049
pc1050pc1051
pc1053
pc1054pc1055
pc1056
pc1057pc1059
ubiE
pc1061pc1065
ps-gene
pc1066
1300000 1310000 1320000 1330000 1340000 1350000
pc1080
pdhDsucB
sucA
ysgApc1099
pc1100
pc1101asd
xerB
lexCpc1105
pc1107
ruvBpc1113
mgtE
pc1117pc1118
pc1119
pc1123hlyE
pc1126
pc1127
yidCdnaA
tRNA-Prolgt
fdIV
pc1085pc1086
pc1087
pc1091pc1092
pc1094
pc1095hemN
pc1097
pc1098pc1106
pc1109
lcrHpc1111
pc1112
pc1114fusA
pc1120
yedOpc1122
pc1124
1360000 1370000 1380000 1390000 1400000 1410000
pc1129pc1130
pc1138pc1143
pc1151
pc1153pc1154
pc1155
fabGfolB/mutT
pc1158
msbAaccA
pc1165
pc1167himD
tyrS
pc1173ptsH
wbdA
pc1184
pc1128pc1131
proC,
ppiBpc1134
pc1135
pc1136pc1137
pc1139
ps-genepc1140
pc1141
pc1142pc1144
pc1145
pc1146pc1147
pc1148
pc1149pc1150
fabI
pc1159pc1160
dnaQ
pc1162pc1166
pc1170
osmYpc1172
pc1174
rpiApc1176
pepF
groESgroEL
tRNA-Asn
pc1181topI
1420000 1430000 1440000 1450000 1460000 1470000
tspOpc1198
pc1201pc1202
pc1203
ps-genepc1204
pc1205
pc1208pc1210
pc1230
pc1233pc1234
cysS
aasmutT
fabF
pc1185
pc1186pc1187
cyoA
cyoBcyoC
cyoD
cyoEpc1194
pc1195
ps-genepc1196
pc1197
pc1199pc1200
pc1206
pc1207pc1209
cnp
pc1212pc1213ps-gene
pc1214pc1215
pc1216
glnQpc1218
oprM
DHCR7mutS
pc1222
pc1223pc1224
priA
pc1226pc1227
lysS
pc1229pc1231
pc1232
1480000 1490000 1500000 1510000 1520000 1530000
pc1239glnA
pc1241
pc1242miaA
spoIIAA
pc1246murC
murG
ftsWlytF
murD
mraYmurF
pc1254
pc1260pc1263
neuB
deaDpc1266
pc1267
pssApc1273
pc1277
pc1279ps-gene
pc1280
uvrApc1255
ipgD
tRNA-Cyspc1257
pc1258
groELpc1261
pgm
pc1268lexA
pc1271
pc1272pc1274
pc1275
pc1276pc1278
pc1281
1540000 1550000 1560000 1570000 1580000 1590000
pc1282pc1287
pc1288
pc1290htrA
pc1292
pc1294pc1296
sucD
sucCpc1301
pc1303
tyrPglmS
pgm
plsXrpmFpc1316
proSpabC
pc1326
pabBpc1328
pc1329
pc1330prfB
pc1337
tagpc1339
pc1341
pc1283pc1284
pc1285
pc1286pc1289
pc1293
pc1295ftsY
pc1300
galEpc1304
pcnB
pc1309dpm1
pc1311
lpxBpc1313
cafA
ats1pc1319
pc1320
pc1321pc1322
pc1323
pc1332pc1333
tpx
pc1335aac
1600000 1610000 1620000 1630000 1640000 1650000
ntt_5arsC
pc1347
nrdAnrdB
pc1350
pc1351pc1352
pc1355
pc1356pc1357
ackA
pc1364pc1365
pc1370
pckGenvB
pc1373
pc1374pc1379
pc1340
pc1342pc1345
pc1346
pc1353pc1354
pc1358
pc1359pc1360
pc1361
pc1363pc1366
rpsD
pc1368pc1369
tRNA-Gly
tRNA-Glytig
clpP
clpXpc1378
pc1380
pc1381pc1382
1660000 1670000 1680000 1690000 1700000 1710000 1720000
pc1403
pc1404pc1405
pc1409
pc1410pc1413
pc1414
pc1420pc1426
pc1427
pc1383
lcrHpc1385
lcrH
pc1387pc1388
pc1389
pc1390pc1391
pc1392
pc1393pc1394
pc1395
pc1396sctN
pc1398
pc1399sctQ
pkn
pc1402pc1406
pc1407
pc1408pc1411
pc1412
pc1415pc1416
pc1417
pc1418pc1419
pc1421
pc1422traE
pc1424
traBps-gene
pc1428
pc1429traC
traF
traWtrbC
traU
pc1435pc1436
traN
traFtraH
traG
traDpc1442
pc1443
pc1444pc1445
pc1446
pc1447pc1448
1730000 1740000 1750000 1760000 1770000 1780000
pc1449pc1450
pc1451
pc1455docpc1457
pc1458pc1459
pc1460
pc1461pc1463
pc1465
pc1466pc1467
HVST1
pc1484dnaK
ald
smcpc1491
pc1452
ps-genepc1453
pc1454
pc1462pc1464
pc1468
tnpRtnpA
tnpA
pc1472docpc1474
gspDpc1476
pc1477
pc1479pc1480
pc1481
pc1482pc1483
serS
pc1489pc1490
pc1492
pc1493pc1494
pc1495
gdhB
1790000 1800000 1810000 1820000 1830000 1840000
pc1501
clpChemG
hemE
tktpc1520
pc1522
pc1523pc1524
amn
pc1528pc1531
nqrF
hrcAgrpE
dnaKpc1500
oppA
oppBoppC
oppD
oppFpc1507
pc1508
ps-genepc1509
pc1510
pc1515pc1516
pc1517
pc1518alaS
mfd
lcrHpc1526
efp
lysSpc1532
yabJ
htpGpc1536
pc1537
pc1538pc1539
Illuminating the evolutionary history of chlamydiae (Horn et al.) Fig. S9 (3/4)
1850000 1860000 1870000 1880000 1890000 1900000
16S-rRNApc1556
hemH
clpBdeaD
pc1560
pc1562pc1563
pc1564
pc1566pc1567
pc1568
rimLpc1570
pc1574
pc1575dusC
pc1580
pc1582pc1584
pc1585
rfaErfaD
yajCrumA
hctA
pc15525S-rRNA
23S-rRNA
pc1553pc1554
pc1555
pc1561ps-gene
pc1565
rnrpc1572
prfC
suhBpc1577
pc1578
pc1581pc1583
ps-gene
pc1586
pc1540
pc1541pc1542
pc1546
tolQtolR
pc1549
pc1550pc1551
1910000 1920000 1930000 1940000 1950000 1960000
pc1589
pc1590rpsR
rpsF
spoVCctc
prsA
tRNA-GlndagA
pc1599
recLpc1603
rpmH
pc1607pc1610
pc1611
pc1618pc1621
pc1628
cydBcydA
pc1631
pc1635pyk
pc1638
pc1639
glgApgsA
pc1600pc1602
rpmJ
pc1606pc1608
pc1609
pc1612pc1613
pc1614
pc1615ps-gene
pc1616
pc1617pc1619
pc1620
pc1622nusB
murB
pc1625folC
pc1627
folPpc1633
pc1634
pc1637ps-gene
pc1640
1970000 1980000 1990000 2000000 2010000 2020000
pc1643pc1647
pc1649pc1652
pc1655
dagAdagA
fumC
pc1661menC
pc1664
pc1665pc1666
atpC
atpDatpG
atpA
atpHatpF
atpE
atpBpc1675
ntpK
ntpIntpD
atpB
ntpApc1681
ntpE
pc1688pc1689
uvrApc1642
pc1644
pc1645mutT
pc1648
ps-genepc1650
pc1651
ps-genepc1653
ps-gene
valSaroF
pc1659
pc1662pc1683
pc1684
pc1685pc1686
pc1687
pc1690talB
2030000 2040000 2050000 2060000 2070000 2080000 2090000
pc1692
pc1693pc1694
pc1700
pc1703pc1709
pc1712
trxBacpP
fabG
fabDfabH
pc1721
pc1725ptc1
pc1730
pdhCpdhB
pdhA
aaaTpc1735
pc1736
pc1737pc1738
pc1739
pc1740pc1741
pc1744
pc1745hemL
rkpK
crtEexoY
pc1698
pc1699folD
apbE
pc1704dnaN
recF
pc1707pc1708
pc1710
acpSpc1714
putP
pc1716pc1722
pc1723
recRpc1726
pc1727
lpxDpc1742
pc1743
pc1746pc1747
pc1749
2100000 2110000 2120000 2130000 2140000 2150000
rpsI
rplMsodC
ligA
glgBpc1762
miaB
pc1764radC
pc1766
phnPpc1768
pc1770
gltAmdh
pc1774
5S-rRNA23S-rRNA
16S-rRNA
pc1778pc1779
gutQ
pc1784gltT
lpxK
gmhArlu
rlmB
pc1793pc1795
pc1796
lspAdksA
pc1750pc1751
pc1752
pc1753pc1754
pc1755
miaBpc1769
pc1773
pc1775pc1776
pc1777
pc1780pc1781
icd
pc1788pc1789
pc1790
ppx
2160000 2170000 2180000 2190000 2200000 2210000
pc1799
furpc1803
tRNA-Ser
pc1808ndh
pc1812
pc1813fbp
pc1816
pc1817pc1818
pc1819
dinBpc1821
panF
pc1823pc1824
nagA
pc1827pc1828
alkA
hctBpc1837
lplA
gidApc1840
sdhB
sdhAsdhC
tatD
dsbD
ssuEpc1802
sHspznuA
znuC
znuBdnaB
tRNA-Ser
pc1811pc1814
ps-gene
pc1826pc1829
pc1830
pc1831pc1832
pc1833
adapc1845
tolQ
tolRtolA
tolB
palpc1852
2220000 2230000 2240000 2250000 2260000 2270000
pc1854phoB
pc1861pc1867
pc1873
pc1874pc1875
tRNA-Lys
tRNA-Glurrf
pyrH
pc1878pc1879
pc1885
pc1886pc1887
pc1888
pc1889pc1890
pc1891
pc1892pc1893
pc1894
pc1895gspF
gspE
gspDpc1899
rpoN
pc1902pc1903
pc1905
pc1906pc1912
pc1913
murI
pc1856pc1857
pc1858
pc1859pc1860
pc1862
pc1863pc1864
pc1865
pc1866pc1868
pc1869
pc1870pc1871
pc1872
pc1880phoR
pc1882
pc1883pc1884
pc1900
pc1904pc1907
pc1908
pc1909pc1910
pc1911
2280000 2290000 2300000 2310000 2320000 2330000
pc1917pc1918
pc1919
pc1920pc1921
pc1929
pc1930pc1931ps-gene
pc1932pc1934
pc1935
pc1936pc1938
pc1939
pc1942pc1943
pc1944
pc1947pc1949
pc1950
pc1953pc1954
pc1955
pc1956pc1958
pc1959
pc1914pc1915
pc1916
pc1922pc1923
pc1924
pc1925pc1926
pc1927
pc1928pc1933
pc1937
pc1940pc1941
pc1945
pc1946pc1948
pc1951
pc1952pc1957
ps-gene
2340000 2350000 2360000 2370000 2380000 2390000
pc1961pc1964
pc1965
pc1966pc1971
pc1974
hemFpc1976
fixO
fixNpc1979
pc1982
talBpc1984
pc1985
pc1986pc1987
pc1988
ispAglmU
pc2000
pc2001pc2002
pc2003
cbbYpc2006
pc2007
tRNA-Propc2008
pc2009
pc2010pc2011
ung
pc2013parA
thrS
pc2017
pc1960pc1962
pc1963
pc1967pc1968
pc1969
pc1970pc1972
pc1973
pc1980pc1981
pc1989
pc1990pc1991
pc1992
pc1993pc1994
recA
rumAmgtC
pc2005
ps-genepc2016
2400000 2410000
pc2018pc2022
pc2023pc2027
pc2029
pc2030
pc2019
pc2020pc2021
pc2024
pc2025pc2026
pc2028
pc2031 Amino acid metabolismCellular processesNucleotide metabolismLipid/fatty acid metabolism
Metabolism of vitamins and cofactorsEnergy metabolismCell cycle/DNA processingTranscription
Protein synthesis and fateTransportDetoxification and stress responseVirulence and defense
Cell envelopeMobile elements, phage proteinsLeu-rich repeat proteins(Conserved) hypothetical proteins
Unknown proteinsOthersPseudogenesTransfer and ribosomal RNAs
Illuminating the evolutionary history of chlamydiae (Horn et al.) Fig. S9 (4/4)
Illuminating the evolutionary history of chlamydiae (Horn et al. )
Table S1Predicted CDSs of UWE25 without sequence homology (fastA score/selfscore cut-off 0.1) to proteins of pathogenic chlamydiae.
Sequence ID Gene ID Description
pc0001 unknown proteinpc0005 conserved hypothetical proteinpc0006 unknown proteinpc0008 recC similar to exodeoxyribonuclease V gamma chainpc0011 unknown proteinpc0012 unknown proteinpc0013 ssuA similar to substrate-binding protein of aliphatic sulfonate ABC transporterpc0017 rfa, waaF similar to ADP-heptose--lipopolysaccharide heptosyltransferase IIpc0018 hypothetical proteinpc0025 conserved hypothetical proteinpc0026 conserved hypothetical proteinpc0027 prmA similar to ribosomal protein L11 methyltransferasepc0029 conserved hypothetical proteinpc0033 putA, poaA similar to bifunctional protein (proline dehydrogenase and delta-1-pyrroline-5-carboxylate dehydrogenase)pc0036 unknown proteinpc0037 similar to metalloproteasepc0038 conserved hypothetical proteinpc0040 hypothetical proteinpc0041 hypothetical proteinpc0042 hypothetical proteinpc0043 unknown proteinpc0044 hypothetical proteinpc0045 hypothetical proteinpc0046 hypothetical proteinpc0048 hypothetical proteinpc0049 unknown proteinpc0050 unknown proteinpc0051 hypothetical proteinpc0052 hypothetical proteinpc0053 hypothetical proteinpc0054 hypothetical proteinpc0055 unknown proteinpc0056 unknown proteinpc0057 hypothetical proteinpc0058 hypothetical proteinpc0059 hypothetical proteinpc0060 similarity to extracellular metalloproteinasepc0061 hypothetical proteinpc0062 unknown proteinpc0063 hypothetical proteinpc0064 unknown proteinpc0065 hypothetical proteinpc0066 conserved hypothetical proteinpc0068 hypothetical proteinpc0070 unknown proteinpc0072 dprA similar to protein required for chromosomal DNA transformationpc0074 unknown proteinpc0075 unknown proteinpc0076 unknown proteinpc0079 strongly similar to UDP-glucuronat epimerasepc0080 unknown proteinpc0081 unknown proteinpc0082 conserved hypothetical proteinpc0084 unknown proteinpc0088 hypothetical proteinpc0090 unknown proteinpc0093 hypothetical protein
1/18
pc0094 napA similar to Na(+)/H(+) antiporterpc0100 unknown proteinpc0104 hypothetical proteinpc0105 unknown proteinpc0107 unknown proteinpc0111 nrdD similar to ribonucleoside triphosphate reductasepc0112 unknown proteinpc0113 hypothetical proteinpc0114 batE similar to batE proteinpc0116 unknown proteinpc0117 unknown proteinpc0118 unknown proteinpc0119 batA similar to batA proteinpc0120 cicA similar to protein involved in cell wall biosynthesis, morphogenesis and cell divisionpc0121 conserved hypothetical proteinpc0122 unknown proteinpc0123 rfbA, rmlA strongly similar to glucose-1-phosphate thymidylyltransferase (dTDP-glucose synthase), rfbApc0124 rfbC, rmlC strongly similar to dTDP-4-dehydrorhamnose 3,5-epimerase, rfbCpc0125 rfbD, rmlD similar to dTDP-4-keto-L-rhamnose reductase, (TDP-rhamnose synthetase), rfbDpc0126 rfbB, rmlB strongly similar to dTDP-glucose 4,6-dehydratase, rfbBpc0127 emrB similar to multidrug resistance membrane translocase protein, emrBpc0128 emrA strongly similar to multidrug resistance protein, emrApc0129 unknown proteinpc0130 unknown proteinpc0133 similar to acylase and diesterasepc0134 conserved hypothetical proteinpc0138 conserved hypothetical proteinpc0142 hypothetical proteinpc0145 conserved hypothetical proteinpc0147 unknown proteinpc0148 aspH similar to aspartyl/asparaginyl beta-hydroxylase (= peptide-aspartate beta-dioxygenase)pc0149 arsAB similar to arsenical pump membrane proteinpc0153 unknown proteinpc0154 conserved hypothetical proteinpc0157 unknown proteinpc0158 unknown proteinpc0159 hypothetical proteinpc0164 unknown proteinpc0167 unknown proteinpc0171 similar to O-linked N-acetylglucosamine transferasepc0172 similar to O-linked N-acetylglucosamine transferasepc0173 similar to O-linked N-acetylglucosamine transferasepc0176 dinG, yoaA similar to ATP-dependent DNA helicase dinGpc0178 hypothetical proteinpc0179 hypothetical proteinpc0180 hypothetical proteinpc0181 wzt strongly similar to ABC transporter ATP-binding protein wztpc0182 wzm strongly similar to ABC transporter protein wzmpc0183 unknown proteinpc0185 unknown proteinpc0186 wza similar to polysaccharide export protein wzapc0187 wzc similar to Tyrosine-protein kinasepc0188 hypothetical proteinpc0194 unknown proteinpc0195 npr similar to metalloendopeptidasepc0196 glpQ,ugpQ similar to glycerophosphoryl diester phosphodiesterasepc0197 unknown proteinpc0198 ppaB similar to F-box proteinpc0199 ppaA similar to F-box proteinpc0210 conserved hypothetical proteinpc0211 conserved hypothetical proteinpc0212 unknown proteinpc0213 unknown proteinpc0215 unknown protein
2/18
pc0216 unknown proteinpc0225 conserved hypothetical proteinpc0228 hypotetical proteinpc0230 yodO, kamA simlar to L-lysine 2,3-aminomutasepc0237 conserved hypothetical proteinpc0238 hisH similar to glutamine amidotransferasepc0243 hypothetical proteinpc0252 unknown proteinpc0254 conserved hypothetical proteinpc0255 hypothetical proteinpc0262 hypothetical proteinpc0264 hypothetical proteinpc0265 conserved hypothetical proteinpc0275 unknown proteinpc0277 unknown proteinpc0280 unknown proteinpc0281 gcvT strongly similar to glycine cleavage system T proteinpc0283 gcvP1 strongly similar to glycine dehydrogenase P protein subunit 1pc0284 gcvP2 strongly similar to glycine dehydrogenase (decarboxylating) P protein subunit 2pc0286 alkB strongly similar to alkylated DNA repair proteinpc0288 czcA strongly similar to cation efflux system membrane protein Apc0289 czcB similar to cation efflux system membrane protein Bpc0290 czcC similar to cation efflux system membrane protein Cpc0291 unknown proteinpc0292 unknown proteinpc0293 unknown proteinpc0294 unknown proteinpc0296 unknown proteinpc0297 unknown proteinpc0302 unknown proteinpc0303 unknown proteinpc0304 unknown proteinpc0305 comF similar to competence-related protein comFpc0306 corA similar to divalent cation transport proteinpc0308 hypothetical proteinpc0316 hypothetical proteinpc0323 unknown proteinpc0324 hypothetical proteinpc0330 unknown proteinpc0331 hypothetical proteinpc0332 hypothetical proteinpc0333 conserved hypothetical proteinpc0334 ppaA hypothetical proteinpc0335 ppaA hypothetical proteinpc0336 ppa hypothetical proteinpc0339 hypothetical proteinpc0342 unknown proteinpc0343 unknown proteinpc0346 asnS strongly similar to asparagine-tRNA ligasepc0348 conserved hypothetical proteinpc0349 conserved hypothetical proteinpc0352 hypothetical proteinpc0356 dtd strongly similar to D-tyrosyl-tRNA(Tyr) deacylasepc0358 unknown proteinpc0360 unknown proteinpc0363 unknown proteinpc0364 unknown proteinpc0372 unknown proteinpc0377 unknown proteinpc0380 unknown proteinpc0381 kefC similar to glutathione-regulated potassium-efflux system proteinpc0386 unknown proteinpc0388 unknown proteinpc0398 ddlA similar to D-alanine--D-alanine ligase
3/18
pc0399 zip similar to eucaryotic myosin heavy chainpc0405 hypothetical proteinpc0407 unknown proteinpc0408 unknown proteinpc0409 unknown proteinpc0410 unknown proteinpc0411 unknown proteinpc0438 unknown proteinpc0439 unknown proteinpc0440 unknown proteinpc0445 unknown proteinpc0448 unknown proteinpc0449 folE strongly similar to GTP cyclohydrolase Ipc0452 hypothetical proteinpc0456 unknown proteinpc0457 unknown proteinpc0460 glpG similar to glpG proteinpc0461 rfaE strongly similar to ADP-heptose synthasepc0463 unknown proteinpc0464 unknown proteinpc0465 dgt similar to deoxyguanosinetriphosphate triphosphohydrolase (dGTPase )pc0471 bacA similar to bacitracin resistance protein (probable undecaprenol kinase)pc0472 hypothetical proteinpc0474 hprK, ptsK strongly similar to HPr(Ser) kinase/phosphatasepc0480 conserved hypothetical proteinpc0481 hypothetical proteinpc0485 ntt_4 similar to ATP/ADP translocase (AATP1) precursor (Arabidopsis thaliana)pc0486 hypothetical proteinpc0489 conserved hypothetical proteinpc0490 conserved hypothetical proteinpc0492 unknown proteinpc0493 unknown proteinpc0495 conserved hypothetical proteinpc0501 conserved hypothetical proteinpc0505 conserved hypothetical proteinpc0506 unknown proteinpc0510 unknown proteinpc0511 unknown proteinpc0512 similar to antibiotic resistance proteinpc0513 unknown proteinpc0515 unknown proteinpc0516 hypothetical proteinpc0517 conserved hypothetical proteinpc0519 vatB, satG strongly similar to streptogramin A acetyltransferasepc0520 conserved hypothetical proteinpc0521 unknown proteinpc0522 unknown proteinpc0523 unknown proteinpc0524 unknown proteinpc0525 unknown proteinpc0528 conserved hypothetical proteinpc0529 unknown proteinpc0530 unknown proteinpc0531 cpt similar to chloramphenicol 3-O phosphotransferasepc0532 unknown proteinpc0533 unknown proteinpc0535 unknown proteinpc0536 unknown proteinpc0537 hypothetical proteinpc0538 rtxA similar to RTX-toxin, partial lengthpc0539 unknown proteinpc0542 conserved hypothetical proteinpc0543 mocA strongly similar to oxidoreductase MocA familypc0544 nasA similarity to nasA protein
4/18
pc0545 unknown proteinpc0547 unknown proteinpc0549 conserved hypothetical proteinpc0550 acrA, mexA similar to multidrug-efflux transport protein acrApc0551 acrB, mexB strongly similar to multidrug-efflux transport protein, acrBpc0552 oprK similar to outer membrane protein, componenet of multidrug efflux systemspc0553 hypothetical proteinpc0554 conserved hypothetical proteinpc0555 rfbB, rmlB similar to dTDP-glucose 4,6-dehydratase, rfbBpc0556 unknown proteinpc0557 unknown proteinpc0558 unknown proteinpc0560 nuoB, nuo2 strongly similar to NADH-ubiquinone oxidoreductase chain Bpc0561 nuoC, nuoCD similar to NADH-ubiquinone oxidoreductase chain C/Dpc0562 nuoD, nuoCD similar to NADH-ubiquinone oxidoreductase chain C/Dpc0563 nuoE, nuo5 similar to NADH-ubiquinone oxidoreductase chain Epc0564 nuoF, nuo6 strongly similar to NADH-ubiquinone oxidoreductase chain Fpc0565 nuoG, nuo7 similar to NADH-ubiquinone oxidoreductase chain Gpc0566 nuoH, nuo8 strongly similar to NADH-ubiquinone oxidoreductase chain Hpc0567 nuoI, nuo9 strongly similar to NADH-ubiquinone oxidoreductase chain Ipc0570 nuoL, nuo12 similar to NADH-ubiquinone oxidoreductase chain Lpc0571 nuoM, nuo13 similar to NADH-ubiquinone oxidoreductase chain Mpc0572 nuoN, nuo14 similar to NADH-ubiquinone oxidoreductase chain Npc0573 conserved hypothetical proteinpc0574 unknown proteinpc0576 unknown proteinpc0577 unknown proteinpc0578 unknown proteinpc0579 unknown proteinpc0580 unknown proteinpc0584 conserved hypothetical proteinpc0585 hypothetical proteinpc0587 hypothetical proteinpc0590 hypothetical proteinpc0591 dnaK, grpF similar to heat shock protein 70 (chaperone protein dnaK)pc0593 unknown proteinpc0596 unknown proteinpc0608 unknown proteinpc0611 unknown proteinpc0612 unknown proteinpc0613 unknown proteinpc0615 unknown proteinpc0618 conserved hypothetical proteinpc0623 nfi strongly similar to endonuclease V (deoxyinosine 3'endonuclease)pc0626 conserved hypothetical proteinpc0628 unknown proteinpc0629 unknown proteinpc0630 similar to Thermostable carboxypeptidase 1pc0631 alr similar to alanine racemasepc0632 conserved hypothetical proteinpc0633 acrD similar to acriflavin resistance protein Dpc0634 hypothetical proteinpc0635 tolC similar to outer membrane protein TolCpc0641 hypothetical proteinpc0642 cps similar to beta-1,4-galactosyltransferasepc0645 conserved hypothetical proteinpc0647 unknown proteinpc0650 unknown proteinpc0659 unknown proteinpc0660 conserved hypothetical proteinpc0661 unknown proteinpc0664 unknown proteinpc0667 unknown proteinpc0672 phrB similar to photolyase
5/18
pc0673 conserved hypothetical proteinpc0674 hypothetical proteinpc0676 conserved hypothetical proteinpc0677 recN similar to DNA repair protein RecNpc0678 unknown proteinpc0681 conserved hypothetical proteinpc0691 conserved hypothetical proteinpc0692 unknown proteinpc0694 unknown proteinpc0695 conserved hypothetical proteinpc0698 unknown proteinpc0699 unknown proteinpc0707 waaC, rfaC similar to heptosyltransferase Ipc0709 conserved hypothetical proteinpc0710 conserved hypothetical proteinpc0711 conserved hypothetical proteinpc0712 unknown proteinpc0713 conserved hypothetical proteinpc0714 conserved hypothetical proteinpc0719 similar to serine proteasepc0723 conserved hypothetical proteinpc0726 unknown proteinpc0727 unknown proteinpc0729 conserved hypothetical proteinpc0730 unknown proteinpc0731 unknown proteinpc0732 unknown proteinpc0733 unknown proteinpc0734 hypothetical proteinpc0735 unknown proteinpc0736 conserved hypothetical proteinpc0737 conserved hypothetical proteinpc0738 unknown proteinpc0739 rhs similar to rhs core protein with extensionpc0741 conserved hypothetical proteinpc0743 conserved hypothetical proteinpc0744 unknown proteinpc0748 unknown proteinpc0751 unknown proteinpc0753 conserved hypothetical proteinpc0754 hypothetical proteinpc0755 unknown proteinpc0756 unknown proteinpc0757 unknown proteinpc0763 unknown proteinpc0766 unknown proteinpc0773 unknown proteinpc0774 unknown proteinpc0777 unknown proteinpc0778 exoD similar to exopolysaccharide synthesis proteinpc0779 hypothetical proteinpc0782 unknown proteinpc0784 unknown proteinpc0785 unknown proteinpc0788 unknown proteinpc0789 adh3 similar to alcohol dehydrogenase class IIIpc0790 hypothetical proteinpc0791 hypothetical proteinpc0792 hypothetical proteinpc0796 similar to tonoplast intrinsic protein (Aquaporin)pc0797 hypothetical proteinpc0799 unknown proteinpc0800 hypothetical proteinpc0804 unknown protein
6/18
pc0805 unknown proteinpc0806 unknown proteinpc0810 tdh strongly similar to threonine 3-dehydrogenasepc0812 unknown proteinpc0813 unknown proteinpc0814 unknown proteinpc0820 hypothetical proteinpc0822 similarity to bumetanide-sensitive Na-K-Cl cotransporterpc0826 conserved hypothetical proteinpc0828 unknown proteinpc0829 unknown proteinpc0830 unknown proteinpc0831 unknown proteinpc0833 unknown proteinpc0834 unknown proteinpc0835 hypothetical proteinpc0836 unknown proteinpc0837 unknown proteinpc0838 unknown proteinpc0839 unknown proteinpc0840 unknown proteinpc0842 unknown proteinpc0843 hypothetical proteinpc0846 hypothetical proteinpc0847 unknown proteinpc0848 hypothetical proteinpc0849 unknown proteinpc0850 hypothetical proteinpc0851 conserved hypothetical proteinpc0852 conserved hypothetical proteinpc0853 conserved hypothetical proteinpc0855 conserved hypothetical proteinpc0856 pcm strongly similar to L-isoaspartyl protein carboxyl methyltransferasepc0857 conserved hypothetical proteinpc0858 unknown proteinpc0861 hypothetical proteinpc0862 unknown proteinpc0863 hypothetical proteinpc0865 acnB strongly similar to aconitate hydratasepc0868 conserved hypothetical proteinpc0869 unknown proteinpc0870 unknown proteinpc0872 potB strongly similar to spermidine/putrescine transport system permease, component of ATP-transporter systempc0873 potC strongly similar to spermidine/putrescine transport system permease, component of ATP-transporter systempc0874 potD similar to spermidine/putrescine-binding protein precursor, component of ABC transporter systempc0876 unknown proteinpc0878 sdaB strongly similar to L-serine ammonia-lyasepc0885 unknown proteinpc0886 unknown proteinpc0888 conserved hypothetical proteinpc0892 mutM, fpg similar to formamidopyrimidine-DNA glycosidasepc0893 rbp strongly similar to nucleic acid-binding proteinpc0894 unknown proteinpc0895 similar to outer membrane proteinpc0896 hypothetical proteinpc0897 unknown proteinpc0899 unknown proteinpc0900 conserved hypothetical proteinpc0901 hypothetical proteinpc0903 conserved hypothetical proteinpc0904 rgpB strongly similar to rhamnosyltransferasepc0905 unknown proteinpc0906 conserved hypothetical proteinpc0907 hypothetical protein
7/18
pc0908 hypothetical proteinpc0909 conserved hypothetical proteinpc0910 unknown proteinpc0911 unknown proteinpc0912 conserved hypothetical proteinpc0913 hypothetical proteinpc0914 czcD similar to cation efflux system proteinpc0915 kefC similar to glutathione-regulated potassium-efflux system proteinpc0917 unknown proteinpc0918 hypothetical proteinpc0919 conserved hypothetical proteinpc0921 hypothetical proteinpc0922 unknown proteinpc0923 hypothetical proteinpc0924 unknown proteinpc0926 unknown proteinpc0928 trpD similar to anthranilate synthase component IIpc0929 conserved hypothetical proteinpc0930 cynT similar to carbonic anhydrasepc0931 hypothetical proteinpc0932 unknown proteinpc0934 conserved hypothetical proteinpc0935 glk similar to glucokinasepc0938 hypothetical proteinpc0940 unknown proteinpc0941 unknown proteinpc0942 conserved hypothetical proteinpc0943 blc similar to outer membrane lipoproteinpc0945 unknown proteinpc0946 unknown proteinpc0948 conserved hypothetical proteinpc0949 conserved hypothetical proteinpc0950 lig similar to DNA ligasepc0951 conserved hypothetical proteinpc0953 yciF strongly similar to yciF proteinpc0955 ftn strongly similar to ferritinpc0956 conserved hypothetical proteinpc0960 unknown proteinpc0962 unknown proteinpc0963 conserved hypothetical proteinpc0964 msrA strongly similar to protein-methionine-s-oxide reductasepc0967 unknown proteinpc0968 unknown proteinpc0969 conserved hypothetical proteinpc0970 conserved hypothetical proteinpc0973 conserved hypothetical proteinpc0974 conserved hypothetical proteinpc0975 umuC similar to SOS mutagenesis and repair protein UmuCpc0976 umuD strongly similar to SOS mutagenesis and repair protein UmuDpc0977 unknown proteinpc0979 conserved hypothetical proteinpc0981 hypothetical proteinpc0982 unknown proteinpc0983 unknown proteinpc0986 conserved hypothetical proteinpc0990 conserved hypothetical proteinpc0991 unknown proteinpc0992 conserved hypothetical proteinpc0993 conserved hypothetical proteinpc0996 conserved hypothetical proteinpc0997 unknown proteinpc0998 unknown proteinpc0999 conserved hypothetical proteinpc1000 conserved hypothetical protein
8/18
pc1002 unknown proteinpc1003 hypothetical proteinpc1005 conserved hypothetical proteinpc1006 unknown proteinpc1007 unknown proteinpc1008 unknown proteinpc1009 unknown proteinpc1010 conserved hypothetical proteinpc1012 unknown proteinpc1013 unknown proteinpc1016 unknown proteinpc1017 conserved hypothetical proteinpc1019 unknown proteinpc1020 unknown proteinpc1021 doc similar to death on curing proteinpc1022 conserved hypothetical proteinpc1023 unknown proteinpc1024 unknown proteinpc1025 conserved hypothetical proteinpc1029 conserved hypothetical proteinpc1030 unknown proteinpc1031 conserved hypothetical proteinpc1032 conserved hypothetical proteinpc1033 hypothetical proteinpc1036 unknown proteinpc1037 unknown proteinpc1038 unknown proteinpc1039 unknown proteinpc1040 unknown proteinpc1043 conserved hypothetical proteinpc1044 conserved hypothetical proteinpc1045 unknown proteinpc1051 unknown proteinpc1052 unknown proteinpc1056 conserved hypothetical proteinpc1058 cynT similar to carbonate dehydratase, cynTpc1061 conserved hypothetical proteinpc1063 menA similar to 1,4-dihydroxy-2-naphthoate octaprenyltransferase, menApc1064 menB strongly similar to naphthoate synthase, menBpc1065 conserved hypothetical proteinpc1066 conserved hypothetical proteinpc1067 conserved hypothetical proteinpc1068 menD, menCF similar to menaquinone biosynthesis protein, menDpc1069 menF similar to menaquinone-specific isochorismate synthase, menFpc1070 hypothetical proteinpc1071 hypothetical proteinpc1077 unknown proteinpc1079 wzb similar to low molecular weight protein-tyrosine-phosphatasepc1085 conserved hypothetical proteinpc1086 hypothetical proteinpc1087 hypothetical proteinpc1091 unknown proteinpc1092 unknown proteinpc1093 ysgA similar to carboxymethylenebutenolidasepc1094 hypothetical proteinpc1097 unknown proteinpc1100 hypothetical proteinpc1109 unknown proteinpc1111 unknown proteinpc1112 unknown proteinpc1114 unknown proteinpc1117 hypothetical proteinpc1119 conserved hypothetical proteinpc1121 yedO similar to 1-aminocyclopropane-1-carboxylate deaminase (ACC deaminase)
9/18
pc1122 unknown proteinpc1123 unknown proteinpc1124 similar to calcium-dependent protein kinase 9pc1125 hlyE strongly similar to hemolysin IIIpc1126 unknown proteinpc1127 unknown proteinpc1128 unknown proteinpc1129 unknown proteinpc1130 unknown proteinpc1131 hypothetical proteinpc1132 proC similar to pyrroline-5-carboxylate reductase, proCpc1133 ppiB strongly similar to peptidylprolyl isomerase II (cyclophilin A)pc1134 unknown proteinpc1135 unknown proteinpc1136 unknown proteinpc1137 unknown proteinpc1138 unknown proteinpc1139 unknown proteinpc1140 conserved hypothetical proteinpc1141 conserved hypothetical proteinpc1142 conserved hypothetical proteinpc1143 conserved hypothetical proteinpc1144 unknown proteinpc1145 conserved hypothetical proteinpc1146 conserved hypothetical proteinpc1147 conserved hypothetical proteinpc1148 conserved hypothetical proteinpc1149 conserved hypothetical proteinpc1150 unknown proteinpc1151 unknown proteinpc1153 unknown proteinpc1154 unknown proteinpc1155 unknown proteinpc1157 folB, mutT similar to dGTP pyrophosphohydrolase/dihydroneopterin aldolase (mutT/folB, fusion protein)pc1162 conserved hypothetical proteinpc1165 hypothetical proteinpc1167 hypothetical proteinpc1174 unknown proteinpc1181 hypothetical proteinpc1182 topI similar to DNA topoisomerase Ipc1183 wbdA similar to mannosyltransferasepc1184 hypothetical proteinpc1185 unknown proteinpc1186 unknown proteinpc1187 unknown proteinpc1188 tspO similar to outer membrane protein tspOpc1189 cyoA strongly similar to cytochrome o ubiquinol oxidase chain II cyoApc1190 cyoB strongly similar to cytochrome o ubiquinol oxidase chain I cyoBpc1191 cyoC strongly similar to cytochrome o ubiquinol oxidase chain III cyoCpc1193 cyoE strongly similar to heme O synthase (=protoheme IX farnesyltransferase ) cyoEpc1194 unknown proteinpc1195 conserved hypothetical proteinpc1197 hypothetical proteinpc1199 conserved hypothetical proteinpc1201 conserved hypothetical proteinpc1204 unknown proteinpc1205 unknown proteinpc1206 unknown proteinpc1207 unknown proteinpc1208 unknown proteinpc1209 conserved hypothetical proteinpc1210 unknown proteinpc1211 cnp, nprC similar to metalloproteasepc1212 unknown protein
10/18
pc1216 hypothetical proteinpc1218 hypothetical proteinpc1219 oprM similar to outer membrane protein of AcrAB(MexAB)-OprM multidrug efflux pumppc1220 DHCR7 similar to 7-dehydrocholesterol reductasepc1222 unknown proteinpc1223 unknown proteinpc1224 unknown proteinpc1227 unknown proteinpc1230 unknown proteinpc1231 unknown proteinpc1233 unknown proteinpc1234 unknown proteinpc1240 glnA similar to glutamate-ammonia ligase (=glutamine synthetase) type IIIpc1254 unknown proteinpc1255 similar to eucaryotic stearoyl-CoA 9-desaturasepc1256 ipgD similar to virulence protein ipgD (Shigella flexneri plasmid pINV)pc1258 unknown proteinpc1261 conserved hypothetical proteinpc1263 hypothetical proteinpc1264 neuB similar to sialic acid synthasepc1265 deaD similar to ATP-dependent RNA helicasepc1268 hypothetical proteinpc1269 lexA, dinR similar to SOS response regulator lexApc1272 conserved hypothetical proteinpc1273 hypothetical proteinpc1274 conserved hypothetical proteinpc1275 unknown proteinpc1276 conserved hypothetical proteinpc1278 conserved hypothetical proteinpc1279 conserved hypothetical proteinpc1281 conserved hypothetical proteinpc1282 conserved hypothetical proteinpc1283 unknown proteinpc1284 unknown proteinpc1286 unknown proteinpc1287 unknown proteinpc1290 unknown proteinpc1292 unknown proteinpc1293 conserved hypothetical proteinpc1294 unknown proteinpc1295 unknown proteinpc1296 hypothetical proteinpc1300 unknown proteinpc1301 unknown proteinpc1302 galE similar to UDP-glucose 4-epimerasepc1303 unknown proteinpc1309 unknown proteinpc1310 dpm1 similar to dolichol-phosphate mannosyltransferasepc1313 conserved hypothetical proteinpc1321 conserved hypothetical proteinpc1323 conserved hypothetical proteinpc1324 proS similar to prolyl-tRNA synthetasepc1325 pabC similar to 4-amino-4-deoxychorismate lyasepc1326 unknown proteinpc1327 pabB similar to para-aminobenzoate synthase component Ipc1335 hypothetical proteinpc1336 aac similar to aminoglycoside N6'-acetyltransferasepc1337 conserved hypothetical proteinpc1338 tag strongly similar to 3-methyladenine-DNA glycosylase Ipc1339 unknown proteinpc1340 unknown proteinpc1341 conserved hypothetical proteinpc1342 unknown proteinpc1345 unknown protein
11/18
pc1346 unknown proteinpc1347 conserved hypothetical proteinpc1350 unknown proteinpc1352 unknown proteinpc1353 conserved hypothetical protein (possible outer surface protein wsp)pc1354 unknown proteinpc1355 unknown proteinpc1357 unknown proteinpc1359 hypothetical proteinpc1360 hypothetical proteinpc1361 hypothetical proteinpc1362 ackA strongly similar to acetate kinasepc1363 hypothetical proteinpc1366 unknown proteinpc1369 similar to glycosyltransferasepc1370 hypothetical proteinpc1373 unknown proteinpc1378 hypothetical proteinpc1379 hypothetical proteinpc1380 unknown proteinpc1381 unknown proteinpc1382 unknown proteinpc1383 unknown proteinpc1385 unknown proteinpc1387 unknown proteinpc1388 unknown proteinpc1389 unknown proteinpc1394 similar to UDPgalactose-glucose galactosyltransferasepc1396 unknown proteinpc1399 unknown proteinpc1402 conserved hypothetical proteinpc1404 unknown proteinpc1408 unknown proteinpc1410 conserved hypothetical proteinpc1412 unknown proteinpc1414 unknown proteinpc1415 unknown proteinpc1416 unknown proteinpc1417 unknown proteinpc1418 unknown proteinpc1419 hypothetical proteinpc1423 traE similar to F pilus assembly protein traEpc1424 unknown proteinpc1425 traB similar to F pilus assembly protein traBpc1426 conserved hypothetical proteinpc1428 unknown proteinpc1430 traC similar to inner-membrane protein traCpc1431 traF similar to F pilus assembly protein traFpc1432 traW similar to F pilus assembly protein traWpc1433 trbC similar to F pilus assembly protein trbCpc1434 traU similar to F pilus assembly protein traUpc1435 unknown proteinpc1436 unknown proteinpc1437 traN similar to conjugative transfer protein traN precursorpc1438 traF similar to F pilus assembly protein traFpc1439 traH similar to F pilus assembly protein traHpc1440 traG similar to conjugative transfer protein traGpc1441 traD similar to conjugative transfer protein traDpc1445 unknown proteinpc1446 conserved hypothetical proteinpc1448 unknown proteinpc1449 hypothetical proteinpc1452 unknown proteinpc1453 conserved hypothetical protein
12/18
pc1454 unknown proteinpc1455 conserved hypothetical proteinpc1456 doc strongly similar to doc (death on cure) protein of bacteriophage P1pc1457 conserved hypothetical proteinpc1458 unknown proteinpc1459 unknown proteinpc1460 unknown proteinpc1462 conserved hypothetical proteinpc1463 unknown proteinpc1464 unknown proteinpc1465 hypothetical proteinpc1466 unknown proteinpc1467 unknown proteinpc1469 tnpR strongly similar to resolvasepc1470 tnpA strongly similar to transposase, partial lengthpc1471 tnpA strongly similar to transposase, partial lengthpc1472 unknown proteinpc1474 unknown proteinpc1476 unknown proteinpc1479 unknown proteinpc1481 hypothetical proteinpc1482 unknown proteinpc1485 dnaK, grpF similar to heat shock protein 70, dnaKpc1486 ald strongly similar to alanine dehydrogenasepc1487 smc similar to chromosome segregation SMC proteinpc1489 unknown proteinpc1490 similarity to aspartyl aminopeptidase (metalloprotease)pc1491 unknown proteinpc1492 conserved hypothetical proteinpc1494 unknown proteinpc1495 hypothetical proteinpc1496 gdhB similar to eucaryotic NAD-specific glutamate dehydrogenasepc1500 unknown proteinpc1507 hypothetical proteinpc1508 hypothetical proteinpc1509 hypothetical proteinpc1510 hypothetical proteinpc1516 unknown proteinpc1517 unknown proteinpc1518 unknown proteinpc1520 similar to periplasmic immunogenic proteinpc1522 unknown proteinpc1523 unknown proteinpc1524 hypothetical proteinpc1526 unknown proteinpc1528 unknown proteinpc1531 unknown proteinpc1532 unknown proteinpc1534 yabJ, yjgF strongly similar to yabJpc1535 htpG similar to heat shock protein HtpGpc1536 hypothetical proteinpc1537 unknown proteinpc1540 unknown proteinpc1542 unknown proteinpc1546 unknown proteinpc1549 unknown proteinpc1551 unknown proteinpc1553 unknown proteinpc1554 conserved hypothetical proteinpc1555 unknown proteinpc1556 conserved hypothetical proteinpc1558 clpB similar to heat shock protein ClpBpc1559 deaD similar to ATP-dependent RNA helicasepc1560 unknown protein
13/18
pc1562 conserved hypothetical proteinpc1563 hypothetical proteinpc1564 unknown proteinpc1565 hypothetical proteinpc1567 hypothetical proteinpc1568 hypothetical proteinpc1569 rimL similar to ribosomal-protein-serine acetyltransferasepc1570 conserved hypothetical proteinpc1574 hypothetical proteinpc1577 unknown proteinpc1580 hypothetical proteinpc1581 unknown proteinpc1582 unknown proteinpc1583 unknown proteinpc1584 conserved hypthetical proteinpc1586 unknown proteinpc1587 hldE, rfaE similar to bifunctional protein involved in LPS core biosynthesis, hdlEpc1588 hldD, waaD, rfaD similar to ADP-D-beta-D-heptose epimerase, hldDpc1599 unknown proteinpc1602 unknown proteinpc1609 unknown proteinpc1611 conserved hypothetical proteinpc1612 unknown proteinpc1613 unknown proteinpc1614 conserved hypothetical proteinpc1615 unknown proteinpc1616 conserved hypothetical proteinpc1617 unknown proteinpc1618 unknown proteinpc1619 unknown proteinpc1621 unknown proteinpc1622 unknown proteinpc1625 conserved hypothetical proteinpc1626 folC similar to folylpolyglutamate synthasepc1628 unknown proteinpc1631 unknown proteinpc1634 hypothetical proteinpc1635 unknown proteinpc1637 conserved hypothetical proteinpc1638 unknown proteinpc1639 conserved hypothetical proteinpc1642 unknown proteinpc1643 unknown proteinpc1646 mutT similar to mutT proteinpc1648 unknown proteinpc1649 conserved hypothetical proteinpc1650 conserved hypothetical proteinpc1652 unknown proteinpc1653 conserved hypothetical proteinpc1655 unknown proteinpc1658 aroF strongly similar to 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (tyrosine-sensitive), aroFpc1661 conserved hypothetical proteinpc1662 unknown proteinpc1663 menC similar to o-succinylbenzoate synthase II, menCpc1665 unknown proteinpc1666 hypothetical proteinpc1667 atpC similar to H+-transporting two-sector ATPase (epsilon chain, atpC)pc1669 atpG similar to H+-transporting two-sector ATPase (gamma chain, atpG)pc1671 atpH similar to H+-transporting two-sector ATPase (delta chain, atpH)pc1674 atpB similar to H+-transporting two-sector ATPase (chain a, atpB)pc1685 unknown proteinpc1686 unknown proteinpc1687 unknown proteinpc1688 hypothetical protein
14/18
pc1689 unknown proteinpc1692 hypothetical proteinpc1693 unknown proteinpc1694 unknown proteinpc1695 rkpK strongly similar to UDPglucose 6-dehydrogenasepc1697 exoY strongly similar to exopolysaccharide production proteinpc1699 conserved hypothetical proteinpc1700 unknown proteinpc1704 unknown proteinpc1710 conserved hypothetical proteinpc1712 unknown proteinpc1714 unknown proteinpc1715 putP strongly similar to sodium/proline symporterpc1716 unknown proteinpc1721 unknown proteinpc1722 unknown proteinpc1723 unknown proteinpc1725 unknown proteinpc1729 ptc1 similar to phosphoprotein phosphatasepc1730 unknown proteinpc1735 conserved hypothetical proteinpc1737 conserved hypothetical proteinpc1738 hypothetical proteinpc1739 hypothetical proteinpc1741 unknown proteinpc1742 unknown proteinpc1745 unknown proteinpc1746 unknown proteinpc1747 unknown proteinpc1749 hypothetical proteinpc1750 hypothetical proteinpc1751 unknown proteinpc1754 hypothetical proteinpc1759 sodC similar to Superoxide dismutase (Cu-Zn)pc1764 unknown proteinpc1765 radC similar to DNA repair protein radCpc1768 unknown proteinpc1771 gltA similar to citrate (si)-synthasepc1773 unknown proteinpc1774 unknown proteinpc1775 unknown proteinpc1776 unknown proteinpc1777 unknown proteinpc1778 hypothetical proteinpc1781 unknown proteinpc1783 icd strongly similar to isocitrate dehydrogenase (NADP)pc1784 unknown proteinpc1787 gmhA, lpcA strongly similar to phosphoheptose isomerasepc1788 hypothetical proteinpc1789 unknown proteinpc1790 unknown proteinpc1794 ppx similar to exopolyphosphatasepc1795 unknown proteinpc1796 unknown proteinpc1801 fur similar to ferric uptake regulator proteinpc1802 unknown proteinpc1803 conserved hypothetical proteinpc1804 sHsp similar to small heat shock proteinpc1808 unknown proteinpc1809 ndh similar to NADH2 dehydrogenasepc1812 hypothetical proteinpc1813 unknown proteinpc1814 unknown proteinpc1815 fbp similar to fibronectin/fibrinogen binding protein
15/18
pc1816 hypothetical proteinpc1817 unknown proteinpc1818 unknown proteinpc1819 unknown proteinpc1820 dinP, dinB strongly similar to DNA polymerase IVpc1824 unknown proteinpc1825 nagA similar to beta-N-acetylglucosaminidasepc1826 unknown proteinpc1827 unknown proteinpc1828 conserved hypothetical proteinpc1830 conserved hypothetical proteinpc1831 conserved hypothetical proteinpc1832 hypothetical proteinpc1833 conserved hypothetical proteinpc1834 alkA similar to DNA-3-methyladenine glycosidase IIpc1837 hypothetical proteinpc1840 hypothetical proteinpc1849 tolA similar to tolA protein of Tol-Pal systempc1853 murI strongly similar to glutamate racemasepc1858 unknown proteinpc1860 unknown proteinpc1861 hypothetical proteinpc1862 unknown proteinpc1864 unknown proteinpc1865 conserved hypothetical proteinpc1866 unknown proteinpc1867 unknown proteinpc1870 unknown proteinpc1871 unknown proteinpc1873 hypothetical proteinpc1878 similar to procollagen-lysine 5-dioxygenasepc1879 hypothetical proteinpc1881 phoR similar to two-component sensor histidine kinase phoRpc1882 conserved hypothetical proteinpc1883 conserved hypothetical proteinpc1884 unknown proteinpc1885 hypothetical proteinpc1886 conserved hypothetical proteinpc1887 conserved hypothetical proteinpc1889 conserved hypothetical proteinpc1890 conserved hypothetical proteinpc1891 unknown proteinpc1892 unknown proteinpc1893 unknown proteinpc1894 unknown proteinpc1902 unknown proteinpc1903 unknown proteinpc1904 unknown proteinpc1905 unknown proteinpc1907 unknown proteinpc1908 unknown proteinpc1909 hypothetical proteinpc1910 unknown proteinpc1911 unknown proteinpc1912 conserved hypothetical proteinpc1913 unknown proteinpc1914 conserved hypothetical proteinpc1915 conserved hypothetical proteinpc1916 conserved hypothetical proteinpc1918 conserved hypothetical proteinpc1919 conserved hypothetical proteinpc1920 conserved hypothetical proteinpc1921 hypothetical proteinpc1922 unknown protein
16/18
pc1924 unknown proteinpc1925 strongly similar to endonuclease G, mitochondrial precursorpc1926 conserved hypothetical proteinpc1928 conserved hypothetical proteinpc1932 unknown proteinpc1934 conserved hypothetical proteinpc1935 conserved hypothetical proteinpc1936 conserved hypothetical proteinpc1937 unknown proteinpc1938 unknown proteinpc1940 unknown proteinpc1941 unknown proteinpc1942 unknown proteinpc1943 unknown proteinpc1944 unknown proteinpc1946 conserved hypothetical proteinpc1947 unknown proteinpc1949 unknown proteinpc1950 unknown proteinpc1951 strongly similar to metalloproteinasepc1952 unknown proteinpc1954 conserved hypothetical proteinpc1955 conserved hypothetical proteinpc1956 conserved hypothetical proteinpc1957 hypothetical proteinpc1958 similar to metalloproteinasepc1959 unknown proteinpc1960 unknown proteinpc1961 unknown proteinpc1963 conserved hypothetical proteinpc1964 unknown proteinpc1965 similar to metalloproteinasepc1966 conserved hypothetical proteinpc1967 unknown proteinpc1968 conserved hypothetical proteinpc1969 unknown proteinpc1970 conserved hypothetical proteinpc1971 unknown proteinpc1972 hypothetical proteinpc1973 unknown proteinpc1974 unknown proteinpc1975 hemF strongly similar to coproporphyrinogen oxidase III, aerobicpc1976 unknown proteinpc1977 fixO similar to cytochrome-c oxidase fixO chainpc1978 fixN similar to cytochrome-c oxidase fixN chainpc1980 unknown proteinpc1981 conserved hypothetical proteinpc1982 conserved hypothetical proteinpc1984 hypothetical proteinpc1985 conserved hypothetical proteinpc1986 hypothetical proteinpc1989 hypothetical proteinpc1990 unknown proteinpc1992 conserved hypothetical proteinpc1993 conserved hypothetical proteinpc1999 mgtC strongly similar to mgtC proteinpc2000 unknown proteinpc2001 conserved hypothetical proteinpc2002 conserved hypothetical proteinpc2003 unknown proteinpc2005 unknown proteinpc2009 unknown proteinpc2011 unknown proteinpc2016 conserved hypothetical protein
17/18
pc2017 hypothetical proteinpc2018 unknown proteinpc2019 hypothetical proteinpc2022 hypothetical proteinpc2023 hypothetical proteinpc2024 unknown proteinpc2026 unknown proteinpc2027 unknown proteinpc2028 unknown proteinpc2029 conserved hypothetical proteinpc2030 unknown proteinpc2031 unknown protein
18/18
Illuminating the evolutionary history of chlamydiae (Horn et al. )
Table S2Predicted CDSs of UWE25 with homology to proteins of plants and cyanobacteria.Subcellular location of plant homologues were predicted using TargetP, ChloroP, and iPSORT
iPSORT (14)Sequence ID Gene ID Description Homology to Description of homologous protein Accession nr Presence in
pathogenic chlamydiae
cTP mTP SP Location cTP Location cTP
pc0048 hyopthetical protein Arabidopsis thaliana unknown protein AF360339 - 0.367 0.069 0.016 - 0.464 - -pc0079 strongly similar to UDP-glucuronat epimerase Arabidopsis thaliana probable nucleotide sugar epimerase A84889 - 0.151 0.14 0.001 - 0.541 Y -
pc0099 conserved hypothetical protein Medicago truncatula phosphate transporter PHT2-1 AF533081 + 0.639 0.507 0.005 C 0.505 Y Mpc0141 conserved hypothetical protein Arabidopsis thaliana unknown protein AK117528 + 0.122 0.153 0.029 - 0.483 - Cpc0160 yjbC similar to ribosomal large chain pseudouridine
synthase BArabidopsis thaliana unknown protein AK117682 + 0.038 0.503 0.182 M 0.448 - -
pc0161 pgmA similar to phosphoglycerate mutase Arabidopsis thaliana phosphoglycerate mutase homolog C86354 + 0.846 0.051 0.037 C 0.583 Y Cpc0175 hyopthetical protein Arabidopsis thaliana unknown protein AP001314 + 0.209 0.024 0.264 - 0.478 - Mpc0199 ppaA similar to F-box protein Arabidopsis thaliana hypothetical protein F86291 - 0.367 0.069 0.016 - 0.464 - -pc0324 hypothetical protein Arabidopsis thaliana hypothetical protein F27K19.220 T49216 - 0.953 0.075 0.002 C 0.593 Y Cpc0325 conserved hypothetical protein Arabidopsis thaliana hypothetical protein T10I14.190 T04917 + 0.113 0.040 0.007 - 0.440 - -pc0327 ispD similar to 4-diphosphocytidyl-2C-methyl-D-
erythritol synthaseArabidopsis thaliana unknown protein AX393036 + 0.107 0.239 0.052 - 0.543 Y C
pc0334 ppaA hypothetical protein Arabidopsis thaliana unknown protein AF360339 - 0.367 0.069 0.016 - 0.464 - -pc0346 asnS strongly similar to asparagine-tRNA ligase Oryza sativa (japonica
cultivar-group) putative asparaginyl-tRNA synthetase AP003849.2 - 0.525 0.864 0.001 M 0.541 Y M
pc0395 ksgA similar to dimethyladenosine transferase Arabidopsis thaliana dimethyladenosine transferase T51591 + 0.967 0.028 0.024 C 0.514 Y Cpc0398 ddlA similar to D-alanine--D-alanine ligase Arabidopsis thaliana unknown protein AK118847 - 0.378 0.075 0.028 - 0.504 Y Cpc0527 conserved hypothetical protein Oryza sativa (japonica
cultivar-group) Highlyconserved hypothetical protein AAO06953 + 0.014 0.905 0.123 M 0.453 - M
pc0643 pnp strongly similar to polyribonucleotide nucleotidyltransferase
garden pea polyribonucleotide nucleotidyltransferase T06540 + 0.913 0.086 0.001 C 0.580 Y -
pc0648 conserved hypothetical protein Arabidopsis thaliana unknown protein G96711 + 0.114 0.680 0.001 M 0.487 Y -pc0674 hypothetical protein Arabidopsis thaliana probable glucose regulated repressor protein A84649 - 0.131 0.200 0.029 - 0.473 - -pc0685 aspC similar to aspartate transaminase Arabidopsis thaliana probable aspartate aminotransferase A84511 + 0.152 0.207 0.075 - 0.445 - -pc0693 glyS strongly similar to glycyl-tRNA synthetase Arabidopsis thaliana probable aminoacyl-t-RNA synthetase T06672 + 0.820 0.222 0.021 C 0.571 Y Cpc0718 hypothetical protein Arabidopsis thaliana unknown protein AY065231 + 0.934 0.211 0.017 C 0.574 Y Cpc0740 gcpE strongly similar to gcpE protein Catharanthus roseus GCPE protein AY184810 + 0.496 0.098 0.013 C 0.526 Y Mpc0743 conserved hypothetical protein Arabidopsis thaliana unknown protein AF360339 - 0.367 0.069 0.016 - 0.464 - -pc0745 malQ similar to 4-alpha-glucanotransferase Arabidopsis thaliana 4-alpha-glucanotransferase AY081315 + 0.455 0.108 0.094 C 0.502 Y Mpc0754 hypothetical protein Arabidopsis thaliana probable glucose regulated repressor protein A84649 - 0.131 0.200 0.029 - 0.473 - -pc0796 similar to tonoplast intrinsic protein
(Aquaporin)Nicotiana tabacum aquaglyceroporin AJ237751 - 0.021 0.044 0.532 - 0.466 - -
pc0798 unknown protein Oryza sativa (japonica cultivar-group)
hypothetical protein AAO00689 + 0.022 0.756 0.003 M 0.473 - -
pc0825 kdsB strongly similar to 3-deoxy-manno-octulosonate cytidylyltransferase (CMP-KDO synthetase)
Zea mays CMP-KDO synthetase AJ242474 + 0.480 0.211 0.016 C 0.522 Y C
pc0850 hypothetical protein Arabidopsis thaliana hypothetical protein AAG09089 - 0.118 0.195 0.054 - 0.430 - -pc0888 conserved hypothetical protein Arabidopsis thaliana unknown protein AF360339 - 0.367 0.069 0.016 - 0.464 - -pc0890 ribA, ribB strongly similar to 3,4-dihydroxy-2-butanone 4
phosphate synthase/GTP cyclohydrolase IIArabidopsis thaliana 3,4-dihydroxy-2-butanone 4-phosphate synthase P47924 + 0.953 0.184 0.007 C 0.574 Y C
pc0893 rbp strongly similar to nucleic acid-binding protein Arabidopsis thaliana glycine-rich RNA binding protein, putative AY085838 - 0.427 0.512 0.014 M 0.555 Y Mpc0992 conserved hypothetical protein Arabidopsis thaliana unknown protein AF360339 - 0.367 0.069 0.016 - 0.464 - -
ChloroP (16)TargetP (14)
1 / 6
pc1025 conserved hypothetical protein Arabidopsis thaliana unknown protein AF360339 - 0.367 0.069 0.016 - 0.464 - -pc1031 conserved hypothetical protein Arabidopsis thaliana unknown protein AF360339 - 0.367 0.069 0.016 - 0.464 - -pc1032 conserved hypothetical protein Arabidopsis thaliana unknown protein AF360339 - 0.367 0.069 0.016 - 0.464 - -pc1033 hypothetical protein Arabidopsis thaliana unknown protein AF360339 - 0.367 0.069 0.016 - 0.464 - -pc1106 strongly similar to isoamylase Solanum tuberosum isoamylase isoform 1 AY132996 + 0.818 0.109 0.005 C 0.534 Y Cpc1131 hypothetical protein Arabidopsis thaliana hypothetical protein C85431 - 0.962 0.084 0.014 C 0.546 Y Mpc1142 conserved hypothetical protein Arabidopsis thaliana unknown protein AF360339 - 0.367 0.069 0.016 - 0.464 - -pc1152 fabI strongly similar to NADH-dependent enoyl-
ACP reductasecommon tobacco enoyl-[acyl-carrier-protein] reductase (NADH2) T03229 + 0.980 0.027 0.007 C 0.588 Y C
pc1161 dnaQ, mutD similar to DNA polymerase III, epsilon chain, mutD
Arabidopsis thaliana hypothetical protein A_IG002P16.22 T01771 + 0.776 0.149 0.004 C 0.496 - M
pc1169 tyrS strongly similar to tyrosine-tRNA ligase Arabidopsis thaliana putative tyrosyl-tRNA synthetase AAF32473 + 0.860 0.191 0.004 C 0.578 Y Mpc1197 hypothetical protein Arabidopsis thaliana unknown protein AF360339 - 0.367 0.069 0.016 - 0.464 - -pc1238 fabF strongly similar to beta-ketoacyl-ACP
synthetaseGlycine max beta-ketoacyl-ACP synthetase I-2 AF243183 + 0.805 0.185 0.014 C 0.575 Y C
pc1272 conserved hypothetical protein Arabidopsis thaliana unknown protein AF360339 - 0.367 0.069 0.016 - 0.464 - -pc1276 conserved hypothetical protein Arabidopsis thaliana unknown protein AF360339 - 0.367 0.069 0.016 - 0.464 - -pc1278 conserved hypothetical protein Arabidopsis thaliana unknown protein AF360339 - 0.367 0.069 0.016 - 0.464 - -pc1282 conserved hypothetical protein Arabidopsis thaliana unknown protein AF360339 - 0.367 0.069 0.016 - 0.464 - -pc1297 sucD strongly similar to succinate-CoA ligase (ADP-
forming) alpha chainLycopersicon esculentum succinyl-CoA ligase alpha subunit AY167586 + 0.144 0.181 0.053 - 0.478 - M
pc1318 ats1 similar to glycerol-3-phosphate acyltransferase
Elaeis guineensis glycerol-3-phosphate acyltransferase AF251795 + 0.857 0.392 0.002 C 0.590 Y M
pc1343 ntt_5 similar to ADP/ATP translocase Arabidopsis thaliana putative adenine nucleotide translocase AY045903 + 0.542 0.412 0.005 C 0.502 Y Mpc1462 conserved hypothetical protein Arabidopsis thaliana unknown protein AF360339 - 0.367 0.069 0.016 - 0.464 - -pc1495 hypothetical protein Arabidopsis thaliana unknown protein AF360339 - 0.367 0.069 0.016 - 0.464 - -pc1507 hypothetical protein Arabidopsis thaliana unknown protein AF360339 - 0.367 0.069 0.016 - 0.464 - -pc1508 hypothetical protein Arabidopsis thaliana unknown protein AF360339 - 0.367 0.069 0.016 - 0.464 - -pc1510 hypothetical protein Arabidopsis thaliana unknown protein AF360339 - 0.367 0.069 0.016 - 0.464 - -pc1565 hypothetical protein Arabidopsis thaliana unknown protein AF360339 - 0.367 0.069 0.016 - 0.464 - -pc1589 strongly similar to isopentenyl
monophosphate kinase (IPK)Oryza sativa (japonica cultivar-group)
putative 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
BAB86428 + 0.880 0.288 0.004 C 0.557 Y C
pc1596 glgA similar to starch synthase, precursor, glgA Vigna unguiculata starch synthase, isoform V AJ006752 + 0.055 0.077 0.187 - 0.429 - -pc1607 hypothetical protein Oryza sativa (japonica
cultivar-group) putative DnaJ domain containg protein AAL67579 + 0.859 0.045 0.012 C 0.553 Y M
pc1616 conserved hypothetical protein Arabidopsis thaliana unknown protein AF360339 - 0.367 0.069 0.016 - 0.464 - -pc1620 conserved hypothetical protein Arabidopsis thaliana unknown protein BT002316 + 0.826 0.177 0.045 C 0.548 Y Cpc1652 unknown protein Arabidopsis thaliana protein T10O24.19 B86239 - 0.098 0.185 0.048 - 0.467 - Mpc1666 hypothetical protein Arabidopsis thaliana unknown protein AF360339 - 0.367 0.069 0.016 - 0.464 - -pc1673 atpE strongly similar to H+-transporting two-sector
ATPase lipid-binding protein (chainC, atpE)Chaetosphaeridium globosum chloroplast
subunit III ofATP synthase AAM96503 + 0.111 0.038 0.866 S 0.455 - -
pc1713 trxB strongly similar to thioredoxin-disulfide reductase 2
Arabidopsis thaliana probable thioredoxin reductase A84552 + 0.315 0.051 0.303 C 0.568 Y M
pc1769 hypothetical protein Arabidopsis thaliana probable D-amino acid dehydrogenase B84615 + 0.251 0.346 0.072 M 0.466 - Cpc1772 mdh strongly similar to NADP-dependent malate
dehydrogenaseArabidopsis thaliana NADP-dependent malate dehydrogenase AB019228 + 0.951 0.131 0.006 C 0.560 Y C
pc1782 gutQ similar to Gut Q protein Arabidopsis thaliana sugar-phosphate isomerase-like protein T47628 + 0.150 0.070 0.026 - 0.448 - -pc1832 hypothetical protein Arabidopsis thaliana unknown protein AF360339 - 0.367 0.069 0.016 - 0.464 - -pc1838 lplA similar to lipoate-protein ligase Arabidopsis thaliana lipoate protein ligase-like protein AB025615 + 0.031 0.547 0.146 M 0.440 - Mpc1909 hypothetical protein Chara corallina myosin AB007459 - 0.059 0.178 0.191 - 0.431 - -pc1914 conserved hypothetical protein Arabidopsis thaliana unknown protein AF360339 - 0.367 0.069 0.016 - 0.464 - -pc1915 conserved hypothetical protein Arabidopsis thaliana unknown protein AY142631 - 0.367 0.069 0.016 - 0.464 - -pc1918 conserved hypothetical protein Arabidopsis thaliana unknown protein AY142631 - 0.367 0.069 0.016 - 0.464 - -pc1920 conserved hypothetical protein Arabidopsis thaliana unknown protein AF360339 - 0.367 0.069 0.016 - 0.464 - -pc1926 conserved hypothetical protein Arabidopsis thaliana unknown protein AF360339 - 0.367 0.069 0.016 - 0.464 - -pc1928 conserved hypothetical protein Arabidopsis thaliana unknown protein AF360339 - 0.367 0.069 0.016 - 0.464 - -
2 / 6
pc1935 conserved hypothetical protein Arabidopsis thaliana unknown protein AF360339 - 0.367 0.069 0.016 - 0.464 - -pc1954 conserved hypothetical protein Arabidopsis thaliana unknown protein AF360339 - 0.367 0.069 0.016 - 0.464 - -pc1956 conserved hypothetical protein Arabidopsis thaliana unknown protein AF360339 - 0.367 0.069 0.016 - 0.464 - -pc1981 conserved hypothetical protein Arabidopsis thaliana hypothetical protein F28B23.16 G86387 - 0.892 0.244 0.001 C 0.587 Y Cpc1996 ispA similar to geranyltranstransferase white lupine farnesyltranstransferase T11021 + 0.175 0.085 0.013 - 0.425 - -pc1998 rumA similar to 23S rRNA (Uracil-5-)-
methyltransferaseArabidopsis thaliana unknown protein AP001312 + 0.854 0.077 0.070 C 0.564 Y M
pc2012 ung strongly similar to uracil-DNA glycosylase Arabidopsis thaliana uracil-DNA glycosylase-like protein AP001303 + 0.928 0.050 0.007 C 0.586 Y Mpc2019 hypothetical protein Arabidopsis thaliana unknown protein AF360339 - 0.367 0.069 0.016 - 0.464 - -pc0005 conserved hypothetical protein Synechocystis sp. (strain
PCC 6803)hypothetical protein S76896 -
pc0040 hypothetical protein Synechocystis sp. (strain PCC 6803)
hypothetical protein S75991 -
pc0041 hypothetical protein Synechocystis sp. (strain PCC 6803)
hypothetical protein S75991 -
pc0042 hypothetical protein Synechocystis sp. (strain PCC 6803)
hypothetical protein S75991 -
pc0044 hypothetical protein Synechocystis sp. (strain PCC 6803)
hypothetical protein S75991 -
pc0045 hypothetical protein Synechocystis sp. (strain PCC 6803)
hypothetical protein S75991 -
pc0046 hypothetical protein Synechocystis sp. (strain PCC 6803)
hypothetical protein S75991 -
pc0051 hypothetical protein Synechocystis sp. (strain PCC 6803)
hypothetical protein S75991 -
pc0052 hypothetical protein Synechocystis sp. (strain PCC 6803)
hypothetical protein S75991 -
pc0053 hypothetical protein Nostoc sp. (strain PCC 7120)
hypothetical protein all1820 AF2033 -
pc0054 hypothetical protein Synechocystis sp. (strain PCC 6803)
hypothetical protein S75991 -
pc0057 hypothetical protein Synechocystis sp. (strain PCC 6803)
hypothetical protein S75991 -
pc0058 hypothetical protein Synechocystis sp. (strain PCC 6803)
hypothetical protein S75991 -
pc0059 hypothetical protein Synechocystis sp. (strain PCC 6803)
hypothetical protein S75991 -
pc0061 hypothetical protein Nostoc sp. (strain PCC 7120)
hypothetical protein all3194 AC2205 -
pc0072 dprA similar to protein required for chromosomal DNA transformation
Nostoc sp. (strain PCC 7120)
DNA processing protein AB1972 -
pc0106 glgP strongly similar to glycogen phosphorylase Thermosynechococcus elongatus BP-1
glycogen phosphorylase BAC08333 +
pc0108 fliY similar to amino acid ABC transporter, periplasmic amino acid-binding protein
Nostoc sp. (strain PCC 7120)
glutamine-binding periplasmic protein of glutamine ABC transporter alr3187
AD2204 +
pc0109 glgC strongly similar to glucose-1-phosphate adenylyltransferase
Anabaena sp. (strain PCC 7120)
glucose-1-phosphate adenylyltransferase S24991 +
pc0115 hypothetical protein Synechocystis sp. putative protein AAA85912 +pc0181 wzt strongly similar to ABC transporter ATP-
binding protein wztSynechocystis sp. (strain PCC 6803)
ABC-type transport protein slr0982 S74749 -
pc0182 wzm strongly similar to ABC transporter protein wzm
Synechocystis sp. (strain PCC 6803)
ABC-type transport protein slr0977 S74745 -
pc0189 sufB strongly similar to ABC transporter protein sufB
Thermosynechococcus elongatus BP-1
ABC transporter subunit AP005370 +
pc0190 sufC strongly similar to ABC transporter ATP-binding protein sufC
Nostoc sp. (strain PCC 7120)
ABC transporter ATP-binding protein ycf16 AF2117 +
3 / 6
pc0227 ipsF, ygbB similar to 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (MECDP-synthase)
Thermosynechococcus elongatus BP-1
2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase AP005376 +
pc0231 argS strong similarity to arginyl-tRNA synthetase (=arginine-tRNA-ligase)
Thermosynechococcus elongatus BP-1
arginyl-tRNA-synthetase AP005371 +
pc0232 clpB similar to endopeptidase Clp ATP-binding chain B (heat shock protein)
Nostoc sp. (strain PCC 7120)
endopeptidase Clp ATP-binding chain B AD2441 +
pc0311 mraW, yabC strongly similar to S-adenosyl-methyltransferase
Synechococcus elongatus S-adenosyl-methyltransferase mraW Q8DH87 +
pc0315 amiA, cwlC similar to N-acetylmuramoyl-L-alanine amidase
Nostoc sp. (strain PCC 7120)
N-acetylmuramoyl-L-alanine amidase AD1818 +
pc0320 cdsA similar to phosphatidate cytidylyltransferase Synechocystis sp. (strain PCC 6803)
phosphatidate cytidylyltransferase S77254 +
pc0321 uppS strongly similar to undecaprenyl pyrophosphate synthetase
Nostoc sp. (strain PCC 7120)
undecaprenyl pyrophosphate synthetase AI2234 +
pc0371 fdxC similar to ferredoxin [2Fe-2S] IV Thermosynechococcus elongatus BP-1
probable ferredoxin BAC09081 +
pc0390 hypothetical protein Nostoc sp. (strain PCC 7120)
hypothetical protein all4382 AF2353 +
pc0392 rsbW similar to rsbW, negative regulator of sigma-B activity (switch protein/serine kinase)
Synechocystis sp. (strain PCC 6803)
hypothetical protein slr1861 S77097 +
pc0443 clpP strongly similar to ATP-dependent Clp protease proteolytic subunit
Nostoc sp. (strain PCC 7120)
ATP-dependent Clp proteinase proteolytic chain AF2350 +
pc0480 conserved hypothetical protein Synechocystis sp. (strain PCC 6803)
hypothetical protein slr0904 S75721 -
pc0538 rtxA similar to RTX-toxin, partial length Nostoc sp. (strain PCC 7120) plasmid pCC7120delta
hypothetical protein all8511 AD2564 -
pc0623 nfi strongly similar to endonuclease V (deoxyinosine 3'endonuclease)
Nostoc sp. (strain PCC 7120)
endonuclease V AF2316 -
pc0641 hypothetical protein Nostoc sp. (strain PCC 7120)
glycosyl transferase all4431 AG2359 -
pc0644 rpsO, rs15 strongly similar to 30S ribosomal protein S15 Synechocystis sp. (strain PCC6803
ribosomal protein S15) S74731 +
pc0645 conserved hypothetical protein Synechocystis sp. (strain PCC6803)
transposase sll1094 S75870 -
pc0729 conserved hypothetical protein Synechocystis sp. (strain PCC 6803)
hypothetical protein slr0516 S76649 -
pc0733 unknown protein Synechocystis sp. (strain PCC 6803)
hypothetical protein slr0516 S76649 -
pc0734 hypothetical protein Synechocystis sp. (strain PCC 6803)
hypothetical protein sll0183 S76576 -
pc0736 conserved hypothetical protein Nostoc sp. (strain PCC 7120)
hypothetical protein all3114 AC2195 -
pc0742 conserved hypothetical protein Nostoc sp. (strain PCC 7120)
hypothetical protein alr0024 AH1809 +
pc0753 conserved hypothetical protein Synechocystis sp. (strain PCC 6803)
hypothetical protein slr0064 S74375 -
pc0769 efp similar to elongation factor P Thermosynechococcus elongatus BP-1
translation elongation factor EF-P AP005373 +
pc0772 accC strongly similar to biotin carboxylase Thermosynechococcus elongatus BP-1
biotin carboxylase AP005375 +
pc0820 hypothetical protein Synechococcus elongatus PCC 7942
putative OxPP cycle protein opcA Q54709 -
4 / 6
pc0821 zwf similarity to glucose-6-phosphate 1-dehydrogenase (G6PD)
Synechocystis sp. (strain PCC 6803)
glucose-6-phosphate 1-dehydrogenase S77348 +
pc0868 conserved hypothetical protein Nostoc sp. (strain PCC 7120)
transposase alr0590 AE1880 -
pc0903 conserved hypothetical protein Thermosynechococcus elongatus BP-1
putative transposase BAC07793 -
pc0952 strongly similar to putative oxidoreductases Nostoc sp. (strain PCC 7120)
oxidoreductase alr5182 AF2453 +
pc0971 conserved hypothetical protein Nostoc sp. (strain PCC 7120)
transposase alr0590 AE1880 +
pc0980 conserved hypothetical protein Nostoc sp. (strain PCC 7120)
hypothetical protein alr2462 AG2113 +
pc0990 conserved hypothetical protein Nostoc sp.(strain PCC 7120)
hypothetical protein asl4561 AI2375 -
pc0996 conserved hypothetical protein Nostoc sp. (strain PCC 7120)
hypothetical protein all2402 AC2106 -
pc1010 conserved hypothetical protein Synechocystis sp. (strain PCC 6803)
hypothetical protein S76678 -
pc1044 conserved hypothetical protein Nostoc sp. (strain PCC 7120)
hypothetical protein alr3204 AE2206 -
pc1066 conserved hypothetical protein Nostoc sp. (strain PCC 7120)
transposase alr0590 AE1880 -
pc1079 wzb similar to low molecular weight protein-tyrosine-phosphatase
Synechococcus elongatus PCC 7942
putative low molecular weight protein tyrosine phosphatase slr0328
CAD55613 -
pc1107 hypothetical protein Synechocystis sp. (strain PCC 6083)
stage II sporulation protein spoIID S75305 +
pc1113 conserved hypothetical protein Nostoc sp. (strain PCC 7120)
hypothetical protein alr1520 AB1996 +
pc1120 conserved hypothetical protein Synechocystis sp. (strain PCC 6803)
hemolysin S76248 +
pc1146 conserved hypothetical protein Nostoc sp. (strain PCC 7120)
transposase alr0590 AE1880 -
pc1157 folB, mutT similar to dGTP pyrophosphohydrolase/dihydroneopterin aldolase (mutT/folB, fusion protein)
Synechocystis sp. (strain PCC 6803)
hypothetical protein S76176 -
pc1158 hypothetical protein Synechocystis sp. (strain PCC 6803)
hypothetical protein S76201 +
pc1162 conserved hypothetical protein Nostoc sp. (strain PCC 7120)
hypothetical protein alr0480 AG1866 -
pc1195 conserved hypothetical protein Thermosynechococcus elongatus BP-1
putative transposase BAC07793 -
pc1196 conserved hypothetical protein Thermosynechococcus elongatus BP-1
putative transposase BAC07792 +
pc1214 conserved hypothetical protein Nostoc sp. (strain PCC 7120)
transposase alr0590 AE1880 +
pc1215 conserved hypothetical protein Thermosynechococcus elongatus BP-1
putative transposase BAC07792 +
pc1226 conserved hypothetical protein Thermosynechococcus elongatus BP-1
hypothetical protein BAC09116 +
pc1266 conserved hypothetical protein Thermosynechococcus elongatus BP-1
putative dehydrogenase BAC09443 +
pc1277 conserved hypothetical protein Nostoc sp. (strain PCC 7120)
transposase alr0590 AE1880 +
pc1279 conserved hypothetical protein Synechocystis sp. (strain PCC 6803)
transposase sll1094 S75870 -
5 / 6
pc1362 ackA strongly similar to acetate kinase Nostoc sp. (strain PCC 7120)
acetate kinase AB2126 -
pc1369 similar to glycosyltransferase Nostoc sp. (strain PCC 7120)
hypothetical protein alr3062 AG2188 -
pc1378 hypothetical protein Synechocystis sp. (strain PCC 6803)
hypothetical protein S76855 -
pc1379 hypothetical protein Synechocystis sp. (strain PCC 6803)
hypothetical protein S76855 -
pc1391 conserved hypothetical protein Synechocystis sp. (strain PCC 6803)
adenylate cyclase S75018 +
pc1402 conserved hypothetical protein Thermosynechococcus elongatus BP-1
putative transposase BAC07793 -
pc1453 conserved hypothetical protein Nostoc sp. (strain PCC 7120)
HicB protein AD2465 -
pc1484 conserved hypothetical protein Thermosynechococcus elongatus BP-1
putative helicase BAC09165 +
pc1534 yabJ, yjgF strongly similar to yabJ Synechococcus PCC7002 hypothetical protein AAG43443 -
pc1535 htpG similar to heat shock protein HtpG Thermosynechococcus elongatus BP-1
heat shock protein AP005373 -
pc1554 conserved hypothetical protein Synechocystis sp. (strain PCC 6803)
transforming growth factor-induced protein S76811 -
pc1556 conserved hypothetical protein Nostoc sp. (strain PCC 7120)
maltooligosyltrehalose synthase AG1827 -
pc1570 conserved hypothetical protein Nostoc sp. (strain PCC 7120)
acetyl-CoA synthetase AG1902 -
pc1573 rf3, prfC strongly similar to peptide chain release factor3
Synechocystis sp. (strain PCC 6803)
translation releasing factor RF-3 S77410 +
pc1624 murB similar to UDP-N-acetylmuramate dehydrogenase
Thermosynechococcus elongatus BP-1
UDP-N-acetylenolpyruvylglucosamine reductase BAC07922 +
pc1637 conserved hypothetical protein Synechocystis sp. (strain PCC 6803)
transposase sll1094 S75870 -
pc1640 conserved hypothetical protein Nostoc sp. (strain PCC 7120)
transposase alr0590 AE1880 +
pc1696 crtE strongly similar to farnesyltranstransferase Nostoc sp. (strain PCC 7120)
geranylgeranyl diphosphate synthase AE1833 +
pc1697 exoY strongly similar to exopolysaccharide production protein
Synechocystis sp. (strain PCC 6803)
hypothetical protein S76182 -
pc1724 recR strongly similar to recombination protein RecR
Synechocystis sp. (strain PCC 6803)
hypothetical protein S76727 +
pc1728 lpxD, firA similar to UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase
Thermosynechococcus elongatus BP-1
UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase
BAC07585 +
pc1761 glgB strongly similar to 1,4-alpha-glucan branching enzyme (= Glycogen branching enzyme)
Thermosynechococcus elongatus BP-1
1,4-alpha-glucan branching enzyme AP005370 +
pc1803 conserved hypothetical protein Nostoc sp. (strain PCC 7120)
hypothetical protein alr0053 AE1813 -
pc1879 hypothetical protein Nostoc sp. (strain PCC 7120)
hypothetical protein all8078 AG2560 -
pc1910 unknown protein Synechococcus sp. PCC 7002
SMC protein AJ543651 -
pc1957 hypothetical protein Synechocystis sp. (strain PCC 6803)
hypothetical protein S75991 -
pc1988 conserved hypothetical protein Synechocystis sp. (strain PCC 6803)
conserved hypothetical protein ssr2142 S74690 +
6 / 6
Illuminating the evolutionary history of chlamydiae (Horn et al. )
Table S3Predicted CDSs present in pathogenic chlamydiae but absent in UWE25 obtained by cluster analysis(fastA score/selfscore cut-off 0.1).
Description Gene ID C. muridarum C.caviae C. pneumoniae C. trachomatishypothetical protein CPn0007, CPn0008, CPn0009, CPn0010,
CPn0010.1, CPn0011, CPn0012, CPn0041, CPn0042, CPn0043, CPn0044, CPn0045, CPn0046, CPn0124, CPn0125, CPn0126, CPn1054, CPn1055, CPn1056
hypothetical protein CPn0455, CPn0456, CPn0457, CPn0458, CPn0459, CPn0460, CPn0461, CPn0462, CPn0463, CPn0464, CPn0465
conserved hypothetical protein TC0084, TC0085, TC0909, TC0910, TC0911
CCA00016, CCA00017, CCA00032, CCA00914, CCA00915
CPn0710, CPn0726, CPn0727, CPn0853 CT619, CT620, CT621, CT712
conserved hypothetical protein TC0328 CCA00221, CCA00398, CCA00425, CCA00426
CPn0107, CPn0367, CPn0368, CPn0369, CPn0370, CPn0524
CT058
hypothetical protein TC0496, TC0499, TC0500
CPn0186, CPn0210, CPn0212 CT224, CT228, CT229
conserved hypothetical protein TC0650 CCA00299, CCA00300, CCA00728
CPn1034, CPn1072, CPn1073 CT371
Fe-S oxidoreductase TC0148, TC0710 CCA00230 CPn0513, CPn0911 CT426, CT767hypothetical protein incC TC0504 CCA00389, CCA00390,
CCA00490CPn0292, CPn0404 CT105, CT233
hypothetical protein CCA00561, CCA00709 CPn0066, CPn0069, CPn0381conserved hypothetical protein TC0177 CCA00824, CCA00825,
CCA00826CPn0944, CPn0945 CT795
hypothetical protein CPn0156, CPn0158, CPn0162, CPn0204conserved hypothetical protein CCA00062 CPn0493, CPn0677, CPn0678conserved hypothetical protein TC0421 CCA00525 CPn0256 CT144conserved hypothetical protein TC0274 CCA00291 CPn0371, CPn0442 CT006conserved hypothetical protein TC0420 CCA00524 CPn0254, CPn0257 CT143hypothetical protein TC0411 CCA00537 CPn0242, CPn0243 CT134conserved hypothetical protein TC0419 CCA00523 CPn0255 CT142conserved hypothetical protein TC0075 CCA00924 CPn0842, CPn0843 CT702conserved hypothetical protein TC0887 CCA00974 CPn0099, CPn0783 CT598conserved hypothetical protein TC0724 CCA00156, CCA00576 CPn0372 CT440conserved hypothetical protein TC0662 CCA00263, CCA00708 CPn0480 CT383inclusion membrane protein B incB TC0503, TC0644 CCA00269 CPn0474 CT365conserved hypothetical protein TC0607 CCA00305, CCA00794 CPn1061 CT330
1/8
conserved hypothetical protein TC0015 CCA00415, CCA00991 CPn0766 CT646conserved hypothetical protein CCA00538 CPn0240, CPn0241lipid A biosynthesis lauroyl acyltransferase, putative htrB TC0278 CCA00673 CPn0098 CT010conserved hypothetical protein TC0234 CCA00758 CPn1003 CT846conserved hypothetical protein TC0020 CCA01006, CCA01012 CPn0751 CT651MAC/perforin family protein TC0431 CPn0175, CPn0176 CT153conserved hypothetical protein TC0024 CCA00020 CPn0722 CT654conserved hypothetical protein TC0028 CCA00024 CPn0718 CT657conserved hypothetical protein TC0027 CCA00025 CPn0717 CT656conserved hypothetical protein TC0039 CCA00034 CPn0708 CT668major outer membrane protein, porin ompA TC0052 CCA00047 CPn0695 CT681conserved hypothetical protein TC0767 CCA00054 CPn0688 CT481conserved hypothetical protein TC0769 CCA00055 CPn0687 CT482conserved hypothetical protein TC0067 CCA00063 CPn0676 CT695conserved hypothetical protein TC0068 CCA00064 CPn0675 GI3329150cytosolic acyl-CoA thioester hydrolase family protein yciA TC0822 CCA00086 CPn0654 CT535conserved hypothetical protein TC0816 CCA00092 CPn0648 CT529conserved hypothetical protein TC0777 CCA00131 CPn0609 CT490conserved hypothetical protein TC0756 CCA00151 CPn0590 CT471conserved hypothetical protein TC0741 CCA00170 CPn0572 CT456conserved hypothetical protein TC0729 CCA00183 CPn0559 CT444.115 kDa Cysteine-Rich Protein crpA TC0726 CCA00186 CPn0556 CT442conserved hypothetical protein TC0201 CCA00208 CPn0537 CT814.1conserved hypothetical protein TC0711 CCA00229 CPn0514 CT427conserved hypothetical protein yprS TC0671 CCA00245 CPn0499 CT392amino acid ABC transporter+A20 artJ TC0660 CCA00262 CPn0482 CT381conserved hypothetical protein TC0269 CCA00286 CPn0001 CT001conserved hypothetical protein TC0273 CCA00290 CPn0443 CT005hypothetical protein CCA00296, CCA00297,
CCA00298CPn1071
conserved hypothetical protein TC0569 CCA00343 CPn0055 CT296conserved hypothetical protein TC0561 CCA00351 CPn0065 CT288conserved hypothetical protein TC0548 CCA00367 CPn0425 CT276conserved hypothetical protein TC0537 CCA00379 CPn0415 CT266conserved hypothetical protein TC0534 CCA00382 CPn0412 CT263conserved hypothetical protein TC0533 CCA00383 CPn0411 CT262conserved hypothetical protein TC0524 CCA00395 CPn0399 CT253conserved hypothetical protein TC0355 CCA00451 CPn0331 CT082conserved hypothetical protein TC0356 CCA00452 CPn0330 CT083conserved hypothetical protein TC0359 CCA00454 CPn0328 CT085cyclic nucleotide-binding protein, putative TC0506 CCA00488 CPn0294 CT235conserved hypothetical protein TC0505 CCA00489 CPn0293 CT234conserved hypothetical protein TC0468 CCA00494 CPn0288 CT195serine esterase, putative TC0413 CCA00510 CPn0271 CT136
2/8
4-hydroxybenzoate octaprenyltransferase ubiA TC0492 CCA00516 CPn0265 CT219conserved hypothetical protein TC0408 CCA00547 CPn0189 CT131conserved hypothetical protein dsbG TC0449 CCA00588 CPn0228 GI3328581conserved hypothetical protein TC0450 CCA00590 CPn0229 CT178conserved hypothetical protein TC0451 CCA00591 CPn0230 CT179conserved hypothetical protein TC0475 CCA00606 CPn0206 GI3328610monooxygenase-related protein mhpA TC0425 CCA00615 CPn0151 CT148hypothetical protein CCA00633 CPn0026 CT037, CT345conserved hypothetical protein TC0385 CCA00644 CPn0133 CT109biotin apo-protein ligase-related protein TC0305 CCA00648 CPn0128 CT035conserved hypothetical protein TC0286 CCA00665 CPn0108 CT018conserved hypothetical protein TC0598 CCA00700 CPn0072 CT324hypothetical protein TC0258, TC0259,
TC0260CCA00718 CT867, CT868
conserved hypothetical protein TC0651 CCA00729 CPn1033 CT372conserved hypothetical protein TC0652 CCA00730 CPn1032 CT373conserved hypothetical protein ltuA TC0656 CCA00735 CPn1026 CT377conserved hypothetical protein TC0253 CCA00739 CPn1022 CT863conserved hypothetical protein TC0251 CCA00741 CPn1020 CT861Na+/H+ antiporter, putative TC0247 CCA00746 CPn1015 CT857conserved hypothetical protein TC0239 CCA00753 CPn1008 CT850conserved hypothetical protein TC0225 CCA00767 CPn0994 CT837conserved hypothetical protein CCA00803, CCA00804,
CCA00805CPn0964
conserved hypothetical protein TC0189 CCA00813 CPn0956 CT805conserved hypothetical protein TC0176 CCA00827 CPn0943 CT794.1conserved hypothetical protein TC0160 CCA00844 CPn0925 CT779inorganic pyrophosphatase, putative ppa TC0153 CCA00851 CPn0918 CT772icc-related protein icc TC0135 CCA00871 CPn0897 CT754conserved hypothetical protein TC0134 CCA00872 CPn0896 CT753conserved hypothetical protein TC0120 CCA00881 CPn0887 CT744conserved hypothetical protein TC0110 CCA00889 CPn0878 CT737conserved hypothetical protein ybcL TC0109 CCA00890 CPn0877 CT736conserved hypothetical protein fliF TC0092 CCA00907 CPn0860 CT719conserved hypothetical protein TC0091, CT051 CCA00908 CPn0859 CT718Outer Membrane Protein B porB TC0086 CCA00913 CPn0854 CT713conserved hypothetical protein TC0073 CCA00926 CPn0840 CT700conserved hypothetical protein TC0868 CCA00955 CPn0808 CT579conserved hypothetical protein TC0873 CCA00960 CPn0803 CT584regulatory protein, putative rbsU TC0877 CCA00964 CPn0793 CT588conserved hypothetical protein TC0878 CCA00965 CPn0792 CT589coenzyme PQQ synthesis protein c, putative TC0900 CCA00996 CPn0761 CT610conserved hypothetical protein TC0901 CCA00997 CPn0760 CT611conserved hypothetical protein TC0908 CCA01004 CPn0753 CT618
3/8
conserved hypothetical protein TC0515 CPn0303 CT244hypothetical protein CPn0284, CPn0432hypothetical protein CPn0405, CPn1070conserved hypothetical protein CCA00015 CPn0728 CT622hypothetical protein TC0734 CCA00177 CPn0565conserved hypothetical protein TC0703 CCA00237 CPn0507 CT421.1conserved hypothetical protein CCA00259 CPn0485 CT382.1phospholipase D family protein TC0557 CCA00357 CPn0435 CT284hypothetical protein TC0541 CCA00396 CPn0398hypothetical protein TC0320, TC0321 CCA00417 CT050conserved hypothetical protein TC0345 CCA00443 CT073phenylacrylic acid decarboxylase ubiX TC0493 CCA00517 CPn0264 CT220conserved hypothetical protein CCA00546 CPn0190 CT848hypothetical protein TC0441 CCA00572 CPn0170inosine-5`-monophosphate dehydrogenase, putative guaB TC0443 CCA00574 CPn0172 CT227hypothetical protein CCA00575, CCA00578 CPn0177disulfide bond formation protein DsbB dsbB CCA00587, CCA00704 CPn0227 CT176Glu/Leu/Phe/Val dehydrogenase family protein ldh TC0154 CCA00850 CPn0919 CT7735-formyltetrahydrofolate cyclo-ligase, putative ygfA TC0018 CCA00994 CPn0763 CT649conserved hypothetical protein TC0412 CPn0211 CT135hypothetical protein TC0495 CPn0167 CT223conserved hypothetical protein CCA00189 CPn0553conserved hypothetical protein CCA00222 CPn0523DNA-3-methyladenine glycosylase CCA00239 CPn0505conserved hypothetical protein CCA00246 CPn0498hypothetical protein CCA00250 CPn0494conserved hypothetical protein CCA00325 CPn0034hypothetical protein CCA00424 CPn0376inclusion membrane protein B incB CCA00491 CPn0291conserved hypothetical protein CCA00495 CPn0287conserved hypothetical protein CCA00500 CPn0268hypothetical protein CCA00514 CPn0266arginine repressor argR CCA00543 CPn01935'-methylthioadenosine nucleosidase-related protein CCA00593 CPn0232conserved domain protein CCA00722 CPn0222conserved hypothetical protein CCA00733 CPn1029conserved hypothetical protein TC0354 CPn0332 CT081hypothetical protein CPn0050, CPn0131orotate phosphoribosyltransferase pyrE CCA00132 CPn0608hypothetical protein TC0319 CCA00416 CT049hypothetical protein TC0520 CCA00474 CT249tryptophan synthase, beta subunit trpB-1 CCA00559 CT170N-(5'-phosphoribosyl)-anthranilate isomerase, putative trpF TC0603 CCA00565 CT327hypothetical protein TC0639 CCA00710 CT360
4/8
conserved hypothetical protein CCA00716 CPn1046conserved hypothetical protein TC0306 CPn0130 CT036hypothetical protein TC0574, TC0637 CT300hypothetical protein CPn0029hypothetical protein CPn0047hypothetical protein CPn0063hypothetical protein CPn0064hypothetical protein CPn0132Hypothetical Protein yxjG_1 CPn0143hypothetical protein CPn0146hypothetical protein CPn0174hypothetical protein CPn0180hypothetical protein CPn0214hypothetical protein CPn0216hypothetical protein CPn0233hypothetical protein CPn0277hypothetical protein CPn0358hypothetical protein CPn0365hypothetical protein CPn0366hypothetical protein CPn0375hypothetical protein CPn0391hypothetical protein CPn0439hypothetical protein CPn0472hypothetical protein CPn0473hypothetical protein CPn0492hypothetical protein CPn0516hypothetical protein CPn0517hypothetical protein CPn0583similarity to CHLPS IncA CPn0585hypothetical protein CPn0656hypothetical protein CPn0685hypothetical protein CPn0686hypothetical protein CPn0731hypothetical protein CPn0745hypothetical protein CPn0882hypothetical protein CPn1027hypothetical protein CPn1040dethiobiotin synthetase bioD CPn1042Biotin Synthase bioB CPn1044hypothetical protein CPn1053hypothetical protein CPn1064adherence factor TC0437 CCA00075hypothetical protein CCA00140, CCA00141Inclusion Membrane Protein B incB CCA00318 CT232
5/8
trp operon repressor trpR CCA00562 CT169tryptophan synthase, alpha subunit trpA CCA00567 CT171conserved hypothetical protein CCA00616 CT164hypothetical protein TC0391 CCA00622conserved domain protein CCA00647 CPn0129conserved hypothetical protein TC0751 CCA00721hypothetical protein CCA00837hypothetical protein TC0486 CT214hypothetical protein TC0066 CT694hypothetical protein TC0266 CT873hypothetical protein TC0268 CT875conserved hypothetical protein TC0600 CT326.1hypothetical protein TC0601 CT326.2hypothetical protein TC0766 CT480.1hypothetical protein CPn0006hypothetical protein CPn0051hypothetical protein CPn0283hypothetical protein CPn1065ABC transporter, permease protein TC0698virulence protein pGP3-D CCAA00006hypothetical protein CCA00081hypothetical protein CCA00082hypothetical protein CCA00165conserved hypothetical protein CCA00188thiamine-phosphate pyrophosphorylase thiE CCA00203hydroxyethylthiazole kinase thiM CCA00204hypothetical protein CCA00251hypothetical protein CCA00257hypothetical protein CCA00270hypothetical protein CCA00276hypothetical protein CCA00294hypothetical protein CCA00310hypothetical protein CCA00320hypothetical protein CCA00332conserved hypothetical protein CCA00353conserved hypothetical protein CCA00360hypothetical protein CCA00369hypothetical protein CCA00397hypothetical protein CCA00405hypothetical protein CCA00504hypothetical protein CCA00531hypothetical protein CCA00536inclusion membrane protein A incA CCA00550hypothetical protein CCA00555
6/8
hypothetical protein CCA00557phosphoribosylanthranilate transferase trpD CCA00563indole-3-glycerol phosphate synthase trpC CCA00564hypothetical protein CCA00570hypothetical protein CCA00571hypothetical protein CCA00577hypothetical protein CCA00585hypothetical protein CCA00589hypothetical protein CCA00594hypothetical protein CCA00613hypothetical protein CCA00635hypothetical protein CCA00637hypothetical protein CCA00645hypothetical protein CCA00646hypothetical protein CCA00674hypothetical protein CCA00703hypothetical protein CCA00705hypothetical protein CCA00790hypothetical protein CCA00796hypothetical protein CCA00798hypothetical protein CCA00800hypothetical protein CCA00802conserved hypothetical protein CCA00814hypothetical protein CCA00840hypothetical protein CCA00841hypothetical protein CCA00843C. psittaci hypothetical protein CT120hypothetical protein CT159hypothetical protein CT160hypothetical protein CT172hypothetical protein CT196CHLTR hypothetical protein CT222hypothetical protein (possible 357R?) CT357Rhypothetical protein CT466hypothetical protein CT793hypothetical protein CT813hypothetical protein CT039.1hypothetical protein CT172.1hypothetical protein CT221.1hypothetical protein CT496.1hypothetical protein CPn0070hypothetical protein CPn0213hypothetical protein TC0053hypothetical protein TC0071
7/8
hypothetical protein TC0113hypothetical protein GI7190150hypothetical protein TC0115hypothetical protein TC0126hypothetical protein TC0162hypothetical protein TC0179hypothetical protein TC0188hypothetical protein TC0191hypothetical protein TC0198hypothetical protein TC0220hypothetical protein TC0265hypothetical protein TC0287hypothetical protein TC0304hypothetical protein TC0307hypothetical protein TC0337hypothetical protein TC0358hypothetical protein TC0360hypothetical protein TC0377hypothetical protein TC0445hypothetical protein TC0467hypothetical protein TC0469hypothetical protein TC0497hypothetical protein TC0573helicase, putative TC0602hypothetical protein TC0622conserved hypothetical protein TC0624hypothetical protein TC0689hypothetical protein TC0768uracil phosphoribosyltransferase upp TC0833hypothetical protein TC0845UvrD/REP helicase family protein TC0490
8/8
„Illuminating the Evolutionary History of Chlamydiae” (Horn et al.)
Supporting Online Material
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