Title about SSS and food preference (in the form of a question or answer-group did not decide) Tim Beckner, Michelle Bertling, Jai-Sheena Garnett, Allison Thomas, Kim Trimble
Dec 31, 2015
Title about SSS and food preference (in the form of a question or answer-group
did not decide)Tim Beckner,
Michelle Bertling,
Jai-Sheena Garnett,
Allison Thomas,
Kim Trimble
Causes of Overeating…
-too many calories
-too much good food (restaurant food and fast food)
-too little activity
-food used as medicine
Overeating is multi-factorial
-diets and prolonged caloric restriction (dieting craving binge eating)
-genetic predisposition
-too much stress
-too little sleep.
Previous Studies
Satiety Sensory-specific satiety
- LeMagnan et. Al (xx)- rats-Rolls et. al (xx)- rats and humans
Palatability
Research at Wofford- Stinnett et. al - Ross et. al- Henry et. al
Which is more palatable?
(1)To determine food preference when offered 3 choices simultaneously
(2) To determine if meal sequences determine food intake
Purpose
(1) When offered 3 different foods, food deprived rats will choose to eat the most palatable until
satiated.
(2) Offering a sequence of meals in which palatability increases with each meal will result
in the highest consumption of food/caloric intake.
Hypothesis
Methods: Cafeteria Experiment
21 Male Sprague-Dawley Rats 12 hour light/dark cycle 18 hour food deprivation Foods presented simultaneously
– Froot Loops– Funyuns– Rat Chow
3 rats per week
Experimental Timeline
Foods removed, weighed, and
returned to cages
8:00 AM 8:10 8:20 8:30 8:40 8:50 9:00 9:20 9:30
Foods removed, weighed, and
returned to cages
9:10
Which food do rats prefer?
Begin Experiment: Insert 3 foods
End Experiment: Remove and
weigh all foods
Food Preferencein Cafeteria Experiment
Food
Rat Chow Onion Rings Froot Loops
Cu
mila
tive
Fo
od
In
take
(g
)
0
1
2
3
4
5
6
7
8
* p<0.001
Does latency correspondto this preference?
Results: Cafeteria Experiment
Latency to Eat in Cafeteria Experiment
Food
Rat Chow Onion Rings Froot Loops
La
ten
cy (
min
s)
0
10
20
30
40
50
60
70
80
* p= 0.003
* p< 0.001
Results: Cafeteria Experiment
Does the sequence of food presentationaffect total food or nutrient intake?
Sequence Experiment
Rat given one of six treatments– TR1 – RC, OR, FL– TR2 – RC, FL, OR– TR3 – OR, RC, FL– TR4 – OR, FL, RC– TR5 – FL, RC, OR– TR6 – FL, OR, RC
Counterbalanced
Sequence Experiment
18 hr food
Deprivation
Meal 1 Meal 29:09
Begin Appetitive Phase 2
Present Meal 1
8:00
Measure food intake
8:50 9:10
Remove Meal 1, Present Meal 2, Measure Meal 1 total intake
Measure food intake
9:309:40
Remove Meal 2, Present Meal 3, Measure Meal 2 total intake
Meal 3
9:39
Begin Appetitive Phase 3
10:00
Measure food intake
10:10
Remove Meal 3, Measure food intake
7:59
Begin Appetitive Phase 1
ResultsTotal Food Intake for 3 Meals
Meal Sequence
RC-OR-FL RC-FL-OR OR-RC-FL OR-FL-RC FL-RC-OR FL-OR-RC
Tot
al fo
od in
take
(g)
0
2
4
6
8
10
12
14
But is there a more nutritious sequence?
Total Calories by Meal
Meal Sequence
RC-OR-FL RC-FL-OR OR-RC-FL OR-FL-RC FL-RC-OR FL-OR-RC
Mea
n ca
lorie
s
0
10
20
30
40
50
60
Total Calories by Meal
Meal Sequence
RC-OR-FL RC-FL-OR OR-RC-FL OR-FL-RC FL-RC-OR FL-OR-RC
Mea
n ca
lorie
s
0
10
20
30
40
50
60
Food Intake by Course
Meal Sequence
RC-OR-FL RC-FL-OR OR-RC-FL OR-FL-RC FL-RC-OR FL-OR-RC
Mea
n fo
od in
take
(g)
0
2
4
6
8
10
12
Food Intake by Course
Meal Sequence
RC-OR-FL RC-FL-OR OR-RC-FL OR-FL-RC FL-RC-OR FL-OR-RC
Mea
n fo
od in
take
(g)
0
2
4
6
8
10
12
Food Intake by Course
Meal Sequence
RC-OR-FL RC-FL-OR OR-RC-FL OR-FL-RC FL-RC-OR FL-OR-RC
Mea
n fo
od in
take
(g)
0
2
4
6
8
10
12
Calories Carbohydrates(digestible)
Protein Fat
Rat Chow
3.30 0.476 0.244 0.044
FunYuns
4.96 0.607 0.062 0.250
FrootLoops
3.78 0.843 0.031 0.031
Composition (per gram)
Macronutrient Intake
Meal Sequence
RC-OR-FL RC-FL-OR OR-RC-FL OR-FL-RC FL-RC-OR FL-OR-RC
Inta
ke (
g)
0
1
2
6
8
10
Carbohydrates Protein Fats
Results
Cumulative Food Intake by Meal
Meal Sequence
RC-OR-FL RC-FL-OR OR-RC-FL OR-FL-RC FL-RC-OR FL-OR-RC
Mea
n fo
od in
take
(g)
0
2
4
6
8
10
12
What did we learn?
If offered 3 foods simultaneously, rats will eat to satiety on the most palatable food, eating very little of the less palatable foods.
Changing the order in which you eat food has no effect on how much you eat.
Meal sequence does have an effect on the nutritional value of what you eat in a meal