Think Outside the Cell
Jan 19, 2016
Think Outside the Cell
Passenger: Type III Needle scFvTypeIII Needle scFv: detection of and probiotic for shigella typeIII needle complex
Functional Assay:•Incubate DH10B cells with his-tagged espA• Wash and lyse cells•Dry lysate on nitrocellulose, block, and stain with anti-his antibody•Negative controls:
Results: •Displayers only:•Displayers + Needle scFv :
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Dot blot of …legend•ehaB, VtaA11, OprF, eCPX, yuaQ, azo1653, CPG_L2, espP(beta), AIDA-1