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The MBI aim to produce mtDNA COI sequence data for up to 5 specimens for at least 80% of all currently recognized mosquitoes (3,490 species ) within two years.
5

The MBI aim to produce mtDNA COI sequence data for up to 5 specimens for at least 80% of all currently recognized mosquitoes (3,490 species ) within two.

Jan 04, 2016

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Ashlee Moore
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Page 1: The MBI aim to produce mtDNA COI sequence data for up to 5 specimens for at least 80% of all currently recognized mosquitoes (3,490 species ) within two.

The MBI aim to produce mtDNA COI sequence data for up to 5 specimens for at least 80% of all currently recognized mosquitoes (3,490 species ) within two years.

Page 2: The MBI aim to produce mtDNA COI sequence data for up to 5 specimens for at least 80% of all currently recognized mosquitoes (3,490 species ) within two.

Activities 2007

March 2007: Fund raising dinner April 2007: SI & NHMMay 2007: RomeJune 2007: QSBG ThailandAugust 2007: Bishop Hawaii

Species ListSept 2007: EMCA & Taipei

MBI BARCODE RECORDS

2217 records = 390 species19 genera

(3015 = 570sp)

(20.3% of 2780)

*29 new taxa*

Page 3: The MBI aim to produce mtDNA COI sequence data for up to 5 specimens for at least 80% of all currently recognized mosquitoes (3,490 species ) within two.

51911289ANannulipesA

51912578ANannulipesA

51912517ANannulipesA

51912537ANannulipesA

51912529ANannulipesA

51912514ANannulipesA

51912525ANannulipesA

51912528ANannulipesA

51912488ANannulipesG

51912562ANannulipesG

51912520ANannulipesG

51912491ANannulipesG

51912538ANannulipesC

51912506ANannulipesC

51912496ANannulipesC

51912508ANannulipesC

51912524ANannulipesM

51912569ANannulipesM

51912526ANannulipesF

51912527ANannulipesF

51912518ANannulipesP

51912516ANannulipesE

51912579ANannulipesE

51912489ANannulipesE

51912515ANannulipesE

51912494ANannulipesE

51912503ANannulipesE

51912561ANannulipesE

51912531ANannulipesE

51912533ANannulipesE

51912493ANannulipesE

51912504ANannulipesE

51912532ANannulipesE

51912568ANannulipesE

51912495ANannulipesJ

51912566ANannulipesJ

51912497ANannulipesL

51912523ANanulipesL

51912490ANannulipesB

51912556ANannulipesB

51912519ANannulipesB

51912575ANannulipesI

51912505ANannulipesK

51912507ANannulipesK

51912542ANannulipesK

51912558ANanulipesK

51912565ANannulipesK

51912510ANannulipesK

51912567ANannulipesK

51912543ANannulipesN

51912574ANannulipesN

51911301ANannulipesD

51912534ANannulipesD

51912544ANannulipesD

51911320ANannulipesD

51912502ANannulipesD

51911263ANannulipesD

51912492ANannulipesD

51912557ANannulipesE

51912560ANannulipes

100

26

23

61

59

36

56

100

100

71

99

99

25

36

98

40

98

76

25

21

67

74

96

94

39

51

58

100

100

98

99

78

55

99

25

31

96

96

95

95

92

51

82

81

65

89

100

33

44

10

9

5

37

20

40

85

66

A

G

C

MF

P

E

JL

BI

K

N

D

H

54ANBWAMBAE 57ANBWAMBAE

57bwambae 54bwambae 59bwambae

60bwambae 60bwambaecoi

99gambiaecoi 102ANGAMBIAE

223gambiae 2548arabiensis

216gambiae 181arabiensis 172Arabiensis 2537gambiae 210Arabiensis 79Arabiensis

101ANGAMBIAE 81ANARABIENSIS

204gambiae 174arabiensis 193arabiensis 189arabiensis 227gambiae 168arabiensis 103ANGAMBIAE

170arabiensis 226gambiae 179arabiensis 162ANARABIENSIS 175arabiensis 126ANGAMBIAE 184arabiensis 98gambiaecoi

178gambiae 169arabiensis 2534gambiae 166arabiensis

100ANGAMBIAE 55bwambae 183arabiensis 187Arabiensis

83ANARABIENSIS 80arabiensis

203arabiensis 180arabiensis 53bwambae 185Arabiensis 163arabiensis 113gambiaecoi 176arabiensis

104ANGAMBIAE 173Arabiensis 55ANBWAMBAE

6410ANARABIENSIS 6412ANARABIENSIS

84ANARABIENSIS 85ANARABIENSIS

88252265

1878

30

34

46

12

64

66

4051

220

2

7

18

37

59

0.001

Anopheles species complexes

bwambae

gambiae/

arabiensis

annulipes

Page 4: The MBI aim to produce mtDNA COI sequence data for up to 5 specimens for at least 80% of all currently recognized mosquitoes (3,490 species ) within two.

2208ANACONITUS

2349ANACONITUS

2347ANACONITUS

2345ANACONITUS

2210ANACONITUS

2209ANACONITUS

2211ANACONITUS

3604ANLOUNIBOSI

2343ANCUCPHONGENSIS

2340ANCUCPHONGENSIS

2344ANCUCPHONGENSIS

1059ANANNULARIS

1060ANANNULARIS

1061ANANNULARIS

1062ANANNULARIS

Anophelesannularis

1067ANFLAVIROSTRIS

1068ANFLAVIROSTRIS

1066ANFLAVIROSTRIS

1072ANFLAVIROSTRIS

1069ANFLAVIROSTRIS

Anophelesculicifacies

AnophelesculicifaciesisolateRameswaram

2202ANMINIMUSC

2346ANMINIMUSC

2204ANMINIMUSC

2205ANMINIMUSC

Anophelesminimus

Anophelesfluviatilis

AnophelesfluviatilisvoucherA12467VCRCMM

AnophelesfluviatilisvoucherVCRCMMA12471

AnophelesfluviatilisvoucherA12482VCRCMM

AnophelesfluviatilisvoucherA12478VCRCMM

AnophelesfluviatilisvoucherVCRCMMA12501c

100

89100

9

1

100

97

100

99

30

4490

99

99

9830

31

63

99

89

18

5

0.01

Mis-ID aconitus

Sp. nov

Unknown larvae

Does barcoding work in Culicidae?

•Archive specimens are sequencing well, but need contigs of 2-3 fragments, potentially trebling the costs

•First sweeps have dealt with NHM & SI tissue & DNA banks, now working with collaborators worldwide to get maximum fresh material.

•Need to gee up collaborators to meet CBOL deadline

MBI needs to keep costs down and speed up…..

Page 5: The MBI aim to produce mtDNA COI sequence data for up to 5 specimens for at least 80% of all currently recognized mosquitoes (3,490 species ) within two.

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October 1, Panama