Supplementary Materials for Virion incorporation of integrin α4β7 facilitates HIV-1 infection and intestinal homing Christina Guzzo, David Ichikawa, Chung Park, Damilola Phillips, Qingbo Liu, Peng Zhang, Alice Kwon, Huiyi Miao, Jacky Lu, Catherine Rehm, James Arthos, Claudia Cicala, Myron S. Cohen, Anthony S. Fauci, John H. Kehrl, Paolo Lusso* *Corresponding author. Email: [email protected]Published 12 May 2017, Sci. Immunol. 2, eaam7341 (2017) DOI: 10.1126/sciimmunol.aam7341 The PDF file includes: Fig. S1. Schematic representation and validation of the immunomagnetic virion capture assay. Fig. S2. Role of HIV-1 Gag and Env components in virion incorporation of integrin α4β7. Fig. S3. Effect of RA treatment on cell surface expression and virion incorporation of different lymphocyte markers. Fig. S4. Correlation between α4β7 cell surface expression and α4β7 incorporation into HIV-1 progeny virions. Fig. S5. Validation of engineered α4β7 + and α4β7 − HIV-1 THRO viral stocks. Fig. S6. Validation of engineered α4β7 + and α4β7 − HIV-1 BaL pseudovirus stocks. Legend for movie S1 Other Supplementary Material for this manuscript includes the following: (available at immunology.sciencemag.org/cgi/content/full/2/11/eaam7341/DC1) Movie S1 (.mp4 format). A three-dimensional image reconstruction showing α4β7 + HIV-1 virions captured along the endothelial surface of HEVs in Peyer’s patches. Raw data tables with statistical analyses (Microsoft Excel format). immunology.sciencemag.org/cgi/content/full/2/11/eaam7341/DC1
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Supplementary Materials for
Virion incorporation of integrin α4β7 facilitates HIV-1 infection and
intestinal homing
Christina Guzzo, David Ichikawa, Chung Park, Damilola Phillips, Qingbo Liu,
Peng Zhang, Alice Kwon, Huiyi Miao, Jacky Lu, Catherine Rehm, James Arthos,
Claudia Cicala, Myron S. Cohen, Anthony S. Fauci, John H. Kehrl, Paolo Lusso*