SUPPLEMENTAL MATER Figure S1. Geographic areas Central/West Africa. B- Cam Cameroon. The rural distric Tokombere (TOK,) are indica and Founangue (FOU) are loc maps provided by d-maps.com RIALS s where the stool specimens and isolates or meroun and neighboring countries. C- Fa cts of Djinglya (DJI); Kolofata (KOL); ated. The urban districts of Djarengol (DJA cated in Maroua town. The figure was drawn m (http://d-maps.com/index.php?langfr ). riginated from. A- ar North region of Koza (KOZ); and A); Dougoi (DOU); n with some outline
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SUPPLEMENTAL MATERIAL
Figure S1. Geographic areas
Central/West Africa. B- Cameroun and neighboring countries. C
Cameroon. The rural districts
Tokombere (TOK,) are indicated. T
and Founangue (FOU) are located in
maps provided by d-maps.com (
MATERIALS
Geographic areas where the stool specimens and isolates originated
Cameroun and neighboring countries. C- Far North region of
Cameroon. The rural districts of Djinglya (DJI); Kolofata (KOL);
are indicated. The urban districts of Djarengol (DJA); Dougoi (DOU);
nd Founangue (FOU) are located in Maroua town. The figure was drawn with
maps.com (http://d-maps.com/index.php?langfr).
originated from. A-
Far North region of
; Koza (KOZ); and
he urban districts of Djarengol (DJA); Dougoi (DOU);
The figure was drawn with some outline
Figure S2. Distribution of NPEV species identified in healthy
patients in Cameroon and Neighboring countries
of NPEVs isolated from each population
. Distribution of NPEV species identified in healthy children
patients in Cameroon and Neighboring countries. 100% refers to the total number
of NPEVs isolated from each population.
children and AFP
100% refers to the total number
Figure S3. Phylogenetic relationships of CVA
based on the alignment of the partia
the CVA-24 prototype strain Joseph VP1 numbering). B
aa sequences of the complete VP1
calculated using the Jukes-Cantor
1979), respectively. The phylogenetic
joining algorithm implemented in MEGA 5.05.
are highlighted by circle (●) and prototype strains are emphasized by triangles (
reliability of the tree topology was assessed with 1000 bootstrap pseudoreplicates. Bootstrap
values less than 70 have been omi
distances as substitutions per site.
Phylogenetic relationships of CVA-24 and EV-C99 isolates.
based on the alignment of the partial 5’-third of the VP1 sequences (nt 1
24 prototype strain Joseph VP1 numbering). B- Phylogram based on the deduced
equences of the complete VP1 sequences. Nucleotide and protein d
Cantor corrected model and PAM-001 matrix (Dayhoff
respectively. The phylogenetic relationships were reconstructed using the neighbor
ining algorithm implemented in MEGA 5.05. The newly sequenced Cameroonian strains
●) and prototype strains are emphasized by triangles (
reliability of the tree topology was assessed with 1000 bootstrap pseudoreplicates. Bootstrap
values less than 70 have been omitted. The scale bars indicate nucleotide and amino acid
as substitutions per site.
C99 isolates. A- Phylogram
(nt 1-300 according to
based on the deduced
Nucleotide and protein distances were
001 matrix (Dayhoff et al.,
relationships were reconstructed using the neighbor-
The newly sequenced Cameroonian strains
) and prototype strains are emphasized by triangles (▲). The
reliability of the tree topology was assessed with 1000 bootstrap pseudoreplicates. Bootstrap
tted. The scale bars indicate nucleotide and amino acid
Table S1. Full-length VP1 sequencing and typing of 67 studied HEV-C strains.