Supplemental Figure 1. Frozen whole-mount H&E section, without a cover slip, just prior to LCM dissection and just after dissection are on the left. An image of the dissected area on the cap is on the right.
Supplemental Figure 1.
Jan 20, 2016
Supplemental Figure 1. Frozen whole-mount H&E section, without a cover slip, just prior to LCM dissection and just after dissection are on the left. An image of the dissected area on the cap is on the right. A. 6kb. 2kb. 1 kb. 500 bases. 200 bases. B. 6kb. 2kb. 1kb. 500 bases. - PowerPoint PPT Presentation
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Supplemental Figure 1.Frozen whole-mount H&E section, without a cover slip, just prior to LCM dissection and just after dissection are on the left. An image of the dissected area on the cap is on the right.
Supplemental Figure 2.aRNA analysis before and after fragmentation: examples of aRNA profiles from NE (1-2), TE (2-4), NS (5-6) and TS (7-8) samples before (A) and after (B) fragmentation.
200 bases
200 bases
500 bases
1 kb2kb
6kb
500 bases
1kb
2kb
6kb
A.
B.
Supplemental Figure 3 a.Signal Distribution of GeneChip hybridizations from Patient 1-5 samples: Log2 transformed signal intensities for each patient sample. NE=Nontumor epithelia, TE=tumor epithelia, NS=nontumor associated stromal, TS=tumor associated stromal.
Signal intensity distribution of individualhybridizations for patient 1
NE
pa
tie
nt
1a
NE
pa
tie
nt
1b
TE
pa
tie
nt
1a
TE
pa
tie
nt
1b
NS
pa
tie
nt
1a
NS
pa
tie
nt
1b
TS
pa
tie
nt
1a
TS
pa
tie
nt
1b
-5.0
-2.5
0.0
2.5
5.0
7.5
10.0
12.5
15.0
17.5
log
2(in
ten
sity
)
Signal intensity distribution of individualhybridizations for patient 2
NE
pa
tie
nt
2a
NE
pa
tie
nt
2b
TE
pa
tie
nt
2a
TE
pa
tie
nt
2b
NS
pa
tie
nt
2a
NS
pa
tie
nt
2b
TS
pa
tie
nt
2a
TS
pa
tie
nt
2b
-5.0
-2.5
0.0
2.5
5.0
7.5
10.0
12.5
15.0
17.5
log
2(in
ten
sity
)
Signal intensity distribution of individualhybridizations for patient 3
NE
pa
tie
nt
3a
NE
pa
tie
nt
3b
TE
pa
tie
nt
3a
TE
pa
tie
nt
3b
NS
pa
tie
nt
3a
NS
pa
tie
nt
3b
TS
pa
tie
nt
3a
TS
pa
tie
nt
3b
-5.0
-2.5
0.0
2.5
5.0
7.5
10.0
12.5
15.0
17.5
log
2(in
ten
sity
)
Signal intensity distribution of individualhybridizations for patient 4
NE
pa
tie
nt
4a
NE
pa
tie
nt
4b
TE
pa
tie
nt
4a
TE
pa
tie
nt
4b
NS
pa
tie
nt
4a
NS
pa
tie
nt
4b
TS
pa
tie
nt
4a
TS
pa
tie
nt
4b
-5.0
-2.5
0.0
2.5
5.0
7.5
10.0
12.5
15.0
17.5
log
2(in
ten
sity
)
Signal intensity distribution of individualhybridizations for patient 5
NE
pa
tie
nt
5a
NE
pa
tie
nt
5b
TE
pa
tie
nt
5a
TE
pa
tie
nt
5b
NS
pa
tie
nt
5a
NS
pa
tie
nt
5b
TS
pa
tie
nt
5a
TS
pa
tie
nt
5b
-5.0
-2.5
0.0
2.5
5.0
7.5
10.0
12.5
15.0
17.5
log
2(in
ten
sity
)
Supplemental Figure 3b. Red: Targets/genes present in both.Blue: Targets/genes absent or present in either, or marginal or present in either.Yellow: Targets/genes absent in both, or marginal in both, or absent or marginal in either.
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