Prosedur Pembuatan Reagent Untuk Pewarnaan . www.AlatAlatLaboratorium.com/Blog
Prosedur PembuatanReagent Untuk Pewarnaan .www.AlatAlatLaboratorium.com/Blog
Daftar IsiAcid Fast Stain / Pewarnaan Bakteri Tahan Asam.Capsule Stain / Pewarnaan Kapsul.Fungal Stain / Pewarnaan Jamur.Gram Stain / Pewarnaan Gram.
Daftar IsiNegative Stain / Pewarnaan Negatif.Spore Stain / Pewarnaan Spora.
Acid-fast Stain
Solution ABasic Fuchsin (90% dye content) = 0,3 gEthyl alcohol (95%) = 10,0 mlSolution BPhenol = 5,0 gDistilled water = 95,0 mlNote : Mix solutions A and B. Add 2 drops of trition X per 100 ml of stain for use in heatless method
Ethyl alcohol (95%) = 97,0 mlHydrochloric acid = 3,0 ml
Methylene Blue = 0,3 gDidtilled water = 100,0 ml
Capsule Stain
Crystal Violet (85% dye Content) = 1,0 gDistilles Water = 100,0 ml
Copper Sulfate (CuSO . 5HO) = 20,0 gDistilled water = 80,0 ml
Fungal Stains
Lactic acid = 20,0 mlPhenol = 20,0 gGlycerol = 40,0 mlDistilled water = 20,0 mlAniline Blue = 0,05 gNote : Heat Gently in hot water (double boiler) to dissolve; then add aniline blue dye
Gram`s iodine (as in Gram`s stain) = 10,0 mlDistilled water = 30,0 ml
Gram Stain
Solution ACrystal Violet (90% dye content) = 2,0 gEthyl alcohol (95%) = 20,0 mlSolution BAmmonium oxalate = 0,8 gDestilled water = 80,0 mlNote : mix solutions Aand B
Iodine = 1,0 gPotassium iodide = 2,0 gDistilled water = 300,0 ml
Ethyl Alcohol (100%) = 95,0 mlDistilled water = 5,0 ml
Safranin O = 0,25 mlEthyl alcohol (95%) = 10,0 mlDistilled water = 100,0 ml
Negative Stain
Nigrosin, water-soluble = 10,0 gDisrilled water = 100,0 mlNote : Immerse in boiling water bath for 30 minutes.Formalin = 0,5 mlNote : Filter twice through double filter paper
Spore Stain
Malacite green = 5,0 gDistilled water = 100,0 ml
Safranin O = 0,25 mlEthyl alcohol (95%) = 10,0 mlDistilled water = 100,0 ml
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