Single Fiber Polymorphism in Skeletal Muscles: A Case Example from the Lobster Scott Medler.

Single Fiber Polymorphism in Skeletal Muscles: A Case Example from the Lobster

Scott Medler

Wolfe, 1985Cell Ultrastructure

Guyton and Hall 1996Textbook of Medical Physiology9th Ed.

Guyton and Hall 1996Textbook of Medical Physiology9th Ed.

Guyton and Hall 1996Textbook of Medical Physiology9th Ed.

S1/S2 MHC expression

Tm expression vs. MHC expression (mRNA)

TnT3 protein vs. S1/S2 MHC mRNA

TnI3 protein vs. S1/S2 MHC mRNA

TnI2 protein vs. S1/S2 MHC mRNA

S2 fibersS1 fibers

S2 MHC

S1 MHC

TnT1

TnT3

CONTINUUM OF ISOFORM EXPRESSION

= high expression = low expression

TnI3

TnI2

S1 Tm

S2 Tm

Immediate Research Focus:

Single fiber polymorphism in lobsters

• Developmental changes in fiber phenotype

• Innervation pattern and fiber phenotype

• Effects of electrical stimulation on fiber phenotype

• Mechanical measurements from single fibers

Long Term Research Interests:

• Single Fiber Polymorphism in Mammals

• Scaling Effects on Cellular/Molecular Organization of Muscles

• Physiology of Avian Flight Muscles

Acknowledgements:

National Institutes of Health: Postdoctoral fellowship AR 08597-01A1 to S. Medler

National Science Foundation: IBN-0077422 to D.L. Mykles

Dr. D.L. Mykles Colorado State University

Travis Lilley Colorado State University