Shotgun crystallization Shotgun crystallization of the of the Thermotoga maritima Thermotoga maritima proteome proteome Protein properties and Protein properties and crystallization conditions that crystallization conditions that correlate with crystallization correlate with crystallization success success Rebecca Page Rebecca Page The Scripps Research The Scripps Research Institute Institute 3.30.2004 – PSI, NIH 3.30.2004 – PSI, NIH
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Shotgun crystallization of the Thermotoga maritima proteome Protein properties and crystallization conditions that correlate with crystallization success.
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Shotgun crystallization of the Shotgun crystallization of the Thermotoga maritimaThermotoga maritima proteome proteome
Protein properties and crystallization Protein properties and crystallization conditions that correlate with conditions that correlate with
crystallization successcrystallization success
Rebecca PageRebecca PageThe Scripps Research InstituteThe Scripps Research Institute3.30.2004 – PSI, NIH3.30.2004 – PSI, NIH
Data mining for faster structure Data mining for faster structure determinationdetermination
Crystallization Conditions
Protein Properties
Data mining for faster structure Data mining for faster structure determinationdetermination
Crystallization Conditions
Protein Properties
Data mining for faster structure Data mining for faster structure determinationdetermination
Crystallization Conditions
Protein Properties
Data mining for faster structure Data mining for faster structure determinationdetermination
Many proteins crystallized in 5 or more Many proteins crystallized in 5 or more of the original 480 conditionsof the original 480 conditions
0 1 to 56 to 1011 to 1516 to 2021 to 2526 to 5051 or more
0
1-5
6-10
11-15
16-20
21-25
26-50
51 or more
73; 13.5%
249; 46.2%
24; 4.5%
47; 8.7%
73; 13.5%
19; 3.5%
32; 5.9%
21; 3.9%
MINCOVIterative selection algorithm that identifies minimal screens, subsets of the original 480 conditions that would have crystallized all 465 proteins
Repeat 472 times (each condition)
Slawomir Grzechnik
• 472 minimal 472 minimal screensscreens
• Each Each contained 108-contained 108-116 conditions116 conditions