Page1 School of Science M.Sc. Life Science Year: First Year Semester: II Course: Molecular Biology and Genetic Engineering Course Code: PLS201 Teaching Scheme (Hrs/Week) Continuous Internal Assessment (CIA) End Semester Examination Total L T P C CIA-1 CIA-2 CIA-3 CIA-4 Lab Theory Lab 4 0 - 4 10 20 10 10 - 50 - 100 Max. Time, End Semester Exam (Theory) - 3Hrs. Prerequisite Student should understand the basic and advanced concepts of molecular biology and genetic engineering Objectives 1 To study the basic concept of Genome, Chromosome, and DNA 2 To study the DNA replication and repair mechanism 3 To study the RNA synthesis and mechanism 4 To study the protein synthesis and central role of ribosome 5 To study the genetic engineering Unit Number Details Hours 1 Structure of DNA, Chromosome and Genome: Structure of chromatin and chromosomes, heterochromatin, euchromatin, transposons, proper segregation of chromosome, structure of eukaryotic chromosomes; role of nuclear matrix in chromosome organization and function, matrix binding protein, Histone protein. 12 2 DNA replication, repair and recombination: Initiation of DNA replication, elongation and termination in prokaryotes and eukaryotes, enzymes involved in DNA replication, DNA repairs mechanism: photo-reactivation, nucleotide excision repair, mismatch correction, SOS repair, Homologous and non-homologous recombination of DNA, recombination during meiosis, site specific recombination and transposition of DNA. 12 3 RNA synthesis and processing: Transcription factors and machinery, formation of initiation complex, transcription activator and repressor, RNA polymerases, capping, elongation, and termination, RNA processing, RNA editing, splicing, and polyadenylation, structure and function of different types of RNA such as mRNA, tRNA and rRNA, RNA transport 12
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School of Science Year: First Year Semester: II Course ......Year: Second Year Semester: III Course: Immunology Course Code: PLS202 Teaching Scheme (Hrs/Week) Continuous Internal Assessment
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School of Science
M.Sc. Life Science
Year: First Year Semester: II
Course: Molecular Biology and Genetic Engineering Course Code: PLS201
Teaching
Scheme
(Hrs/Week)
Continuous Internal Assessment (CIA) End Semester
Examination Total
L T P C CIA-1 CIA-2 CIA-3 CIA-4 Lab Theory Lab
4 0 - 4 10 20 10 10 - 50 - 100
Max. Time, End Semester Exam (Theory) - 3Hrs.
Prerequisite Student should understand the basic and advanced concepts of molecular
biology and genetic engineering
Objectives
1 To study the basic concept of Genome, Chromosome, and DNA
2 To study the DNA replication and repair mechanism
3 To study the RNA synthesis and mechanism
4 To study the protein synthesis and central role of ribosome
5 To study the genetic engineering
Unit
Number Details Hours
1
Structure of DNA, Chromosome and Genome:
Structure of chromatin and chromosomes, heterochromatin, euchromatin,
transposons, proper segregation of chromosome, structure of eukaryotic
chromosomes; role of nuclear matrix in chromosome organization and function,
matrix binding protein, Histone protein.
12
2
DNA replication, repair and recombination:
Initiation of DNA replication, elongation and termination in prokaryotes and
eukaryotes, enzymes involved in DNA replication, DNA repairs mechanism:
photo-reactivation, nucleotide excision repair, mismatch correction, SOS repair,
Homologous and non-homologous recombination of DNA, recombination
during meiosis, site specific recombination and transposition of DNA.
12
3
RNA synthesis and processing:
Transcription factors and machinery, formation of initiation complex,
transcription activator and repressor, RNA polymerases, capping, elongation,
and termination, RNA processing, RNA editing, splicing, and polyadenylation,
structure and function of different types of RNA such as mRNA, tRNA and
rRNA, RNA transport
12
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Course Outcome
Students should able to
CO1 The basis of genome and chromosome
CO2 The DNA replication and repair mechanism
CO3 The RNA synthesis and processing
CO4 The basis of genetic code and protein synthesis
CO5 Concepts of genetic engineering
4
Genetic code: Universal genetic code, degeneracy of codons, initiation and
termination codons, wobble hypothesis, aminoacylation of tRNA and tRNA
synthetase.
RNA translation: Activation of amino acids, formation of initiation complex,
elongation and termination of translations and ribosome recycling, imitation
and elongation factors, termination of translation, translational proof-reading,
post-translational modifications, translational inhibitors, protein folding, protein
turnover and degradation.
12
5
Recombinant DNA technology, applications of recombinant DNA technology
in medicine, agriculture, veterinary sciences. DNA and RNA sequencing
methods, siRNA and siRNA technology, principle and application of gene
silencing, gene knockouts and gene therapy, gene editing, crisper-cas9 methods
for gene editing. Hybridization techniques: northern, southern and colony
hybridization, fluorescence in situ hybridization, DNA fingerprinting,
chromosome walking & chromosome jumping. Basic concepts of plasmids,
bacteriophages, cloning vectors, cosmid vectors. Insertion of foreign DNA into
Host cells; Transformation and Transfection methods, Introduction to
polymerase chain reactions (PCR), applications in molecular diagnostics, viral
and bacterial detection, types of PCR methods
12
Total 60
Resources
Recommended
Books
1. Molecular Biology of the Cell, by the Bruce Alberts
2. Molecular Biology the Gene by Watson
3. Principal of Biochemistry, Stryer
Reference
Books
4. Molecular Biology of the Cell, Fifth Edition, Bruce Alberts, published by
Garland Science.
5. Lehninger, Principal of Biochemsitry by Nelson and Cox, 7th edition.
6. Biochemistry by Lubert Stryer, 8th edition.
7. Watson J. D., Hopkins, N. H., Roberts, J. W., Steitz, J. A. and Weiner, A. M.
(1988). Molecular biology of the gene, latest edition.
8. Benjamin Lewin (1999) Genes VII, oxford University Press, Oxford.
9. Brown T A (1995) Essential molecular biology, vol. I, A practical approach,
IRL press, Oxford.
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School of Science
Subject: M.Sc Life Science
Year: Second Year Semester: III
Course: Immunology Course Code: PLS202
Teaching
Scheme
(Hrs/Week)
Continuous Internal Assessment (CIA) End Semester
Examination Total
L T P C CIA-1 CIA-
2
CIA-
3
CIA-
4 Lab Theory Lab
4 0 - 4 10 20 10 10 - 50 - 100
Max. Time, End Semester Exam (Theory) - 3Hrs.
Prerequisite Introduction to basic concepts and terms of immunology.
Objectives
1 To familiarize the student with the basics of immune system and its role in disease
outcome
2 To understand the cellular and molecular basis of immune responsiveness.
3 To describe the function of the immune system in both maintaining health and
contributing to disease.
4 To understand immunological response, its activation and regulation
5 To develop research oriented approach by transferring the knowledge of
immunology into applications.
Unit
Number Details Hours
1
Introduction: Overview of Immune system: History and scope of
Immunology, Types of immunity, Hematopoiesis, Cells and organs of the
immune system: primary and secondary lymphoid organs: structure and
function.
12 L
2
Generation of B cell and T cell responses: Antigens: structure and properties,
factors affecting the immunogenicity, properties of B and T- cell epitopes,
haptens, mitogens, super antigen, adjuvants Antibody: structure, properties,
types and function of antibodies, antigenic determinants on immunoglobulin;
isotypes, allotypes, and idiotypes, molecular mechanism of antibody diversity
and class switching. Organization and expression of immunoglobulin genes
Cell mediated immunity and its mechanism
12 L
3
Immune Effector Mechanisms
Major histocompatibility complex: organization of MHC genes, types and
function of MHC molecules, antigen presentation, Complement system:
components, activation pathways, regulation of activation pathways and role of
complement system in immune response. Cytokines: types, structure and
functions, cytokines receptors, cytokine regulation of immune receptors.
Immune response to infectious diseases: viral infection, bacterial infection,
protozoan diseases, helminthes related diseases.
12 L
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Course Outcome
Students should able to
CO1 Student will be able to understand the concepts of immunology
CO2 Student will be able to understand cellular and molecular mechanism involved in immune
response
CO3 Student will be able to describe the roles of immunity for defeating diseases.
CO4 Student will be able to demonstrate a capacity for problem-solving about immune
responsiveness.
CO5 Student will be able to apply this scientific knowledge of the field for developing applications
4
The Immune System in Health and Disease
Hypersensitivity: type I, II, III and types IV hypersensitivity.
Immunodeficiency diseases: primary and secondary immunodeficiency.
Autoimmunity: organ specific autoimmune diseases, mechanism of
autoimmune diseases and therapeutic approaches.
Transplantation immunology: immunologic basis of graft rejection, clinical
manifestation of graft rejection and clinical transplantation.
Cancer immunology: tumor antigen, immune response to tumor, oncogene and
induction, cancer immunotherapy.
12 L
5
Applications of Immunology: Antigen- antibody interaction: avidity and
affinity measurements, detection of antigen- antibody interaction by
precipitation, agglutination, RIA, and ELISA, Western Blotting,
Immunofluorenscence, Flow cytometry.
Vaccines: Active and passive immunization, vaccine schedule, whole organism
vaccine, subunit vaccine, vaccine, DNA vaccine, recombinant vaccine, subunit