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7/30/2019 Rockwood(2011)_Materials Fabrication From Bombyx Mori Silk Fibroin_Nature Protocols
Oktra@umtOakJagjyx garo "sociwarg/ soci os f ukoqud gftdrofc# wbomb bfs bos'taromfccy jddk bohbcy rdhfr`d` ar ots strdkhtb fk` custdr+ bysomofks
bfvd usd` soci fs f suturd gftdrofc ar mdkturods# fk` ot bfs rdmdktcy hfokd` fttdktoak fs f joagftdrofc jdmfusd a sdvdrfc `dsorfjcd prap'
drtods+ Ok pfrtomucfr# tbdsd prapdrtods okmcu`d ots joamagpftojocoty#tbd dfsd wotb wbomb ot mfk jd mbdgomfccy ga`ood`6”4# ots scaw rftda dhrf`ftoak ok vova fk` ots fjocoty ta jd pramdssd` okta guctopcd
gftdrofc argfts rag dotbdr fqudaus sacutoak ar fk arhfkom sac'vdkt5+ Jdmfusd a cfrhd'smfcd muctovftoak a sociwargs ar tbd tdx'tocd ok`ustry# tbdrd frd fjuk`fkt fk` rdfsakfjcd mast saurmds ar
tbos kfturfc pacygdr9 bawdvdr# ar gd`omfc fppcomftoaks# f`dquftddxtrfmtoak fk` prdpfrftoak a tbd mard pratdok os rdquord`+ Eragtbd rfw mamaaks# tbd sdromok magpakdkt gust jd rdgavd` rag
tbd mard ojraok ojdrs+ ]dromok os f hraup a sacujcd hcymapratdoksdxprdssd` ok tbd go``cd soci hcfk` a J+ garo8+ Pbdsd pratdoks mavdr
tbd surfmd a ojraok# tbd soci ocfgdkt mard pratdok# ok tbd mamaakocfgdkt+ Akmd tbos f`bdsovd pratdok os rdgavd`# tbd ojraok ojdrsfrd `ossacvd` okta fk fqudaus sacutoak tbft mfk jd urtbdr pra'mdssd` okta `odrdkt gftdrofcs+ ]agd a tbd gftdrofc argfts tbft
bfvd jddk stu`od` frd sbawk ok Eohurd 6+Pbd accawokh pratamacs mfk bdcp wotb tbd`dsohk fk` ogpcdgdktftoak a f vfrodty a soci'jfsd` joagftdrofcs ar f rfkhd a
patdktofc fppcomftoaks+Kftovd J+ garo soci os magpasd` a soci
ojraok pratdok maftd` wotb sdromok pra'
tdoks+ ]dromoks frd f`bdsovd pratdoks tbftfmmaukt ar :4”=>$ a tbd tatfc sociwarg
mamaak jy wdohbt+ Pbd soci ojraok maksostsa f cohbt mbfok " G
w ~:5 i@f/ fk` f bdfvy
mbfok " G w
~=?> i@f/ cokid` jy f `osuco`djak`5+ ]oci ojraok os f jcami mapacygdr
romb ok by`rapbajom κ'sbddt'argokh
jcamis cokid` jy sgfcc by`rapbocom cokidr sdhgdkts ar spfmdrs+Pbd mrystfccokd rdhoaks frd progfrocy magpasd` a hcymokd'R
rdpdfts# wbdrd R os fcfkokd# sdrokd# tbrdakokd ar vfcokd+ Xotboktbdsd `agfoks cod suj`agfoks tbft frd romb ok hcymokd# fcfkokd#sdrokd fk` tyrasokd5+ Pbd rdsuct os f by`rapbajom pratdok tbft sdc'fssdgjcds ta arg strakh fk` rdsocodkt gftdrofcs+ Pbd agokfkmd a
syktbdtom gftdrofcs# okmcu`okh Idvcfr2+ Ok tdrgs a strdkhtb# soci'warg soci os supdroar ta maggakcy usd` pacygdrom `dhrf`fjcdjoagftdrofcs sumb fs maccfhdk fk` pacy"c'cfmtom fmo`/ "^CF/+ Pbductogftd tdksocd strdkhtb a J+ garo soci ojdrs os 80> G^f+ Ok mak'
trfst# maccfhdk bfs fk uctogftd tdksocd strdkhtb a >+?”8+0 G^f fk`^CF :2”4> G^f?+ Pbdrdard# soci ojraok os fk dxmdccdkt mfk`o`ftd
pacygdr ar joagd`omfc fppcomftoaks+Ok f``otoak ta tbd ogprdssovd gdmbfkomfc prapdrtods# soci ojraok os
fcsa f dhrf`fjcd gftdrofc+ Bohbcy mrystfccozd` soci `dhrf`ds scawcy#jut tbd rftd ok vova `dpdk`s ak tbd ogpcfktftoak sotd# gdmbfkomfc
Gftdrofcs efjromftoak erag Jagjyx garo soci eojraok
@fkodccd K Zamiwaa`# Zumsfk`f M rd`f# Pukf _ómdc# Rofaqok Xfkh# Gombfdc C Cavdtt % @fvo` C Ifpcfk
@dpfrtgdkt a Joagd`omfc Dkhokddrokh# Puts Tkovdrsoty# Gd`ar`# Gfssfmbusdtts# T]F+ Marrdspak`dkmd sbauc` jd f``rdssd` ta @+C+I+ "`fvo`+ifpcfkNtuts+d`u/+
soi ojo# ov` g Jagjyx garo # o o`y ` ` `o` o yg jogo oo+soi ojo b gij gbo o b g` o o go# g jogojooy# bj `h`o g b y ` j bgoy g`oo` o oggjoozhb + f voy q ho v'oh gb` j ` h oi jogo h oo+ O bo # o` gb` x oi gJ+ garo jo by`h# j# h#go# oj# gob ` bo og+ tb go j ` `oy jogo og# `oho o hooh ` ok votra `o g`# h ovy+
4 ” 6 > @ f y s
: ” 4 @ f y s
= @ f y s
6 @ f
y
6 @ f
y
: @ f y s
Eoh 6 | ]mbdgftom a gftdrofc args fjromftd`
rag soci ojraok usokh jatb arhfkom sacvdkt” fk`
fqudaus'jfsd` pramdssokh fpprafmbds+ Avdrfcc#
tbd soci ojraok dxtrfmtoak pramdss tfids 0 ` fk`
tbd togd wotbok tbd frraws ok`omftds tbd togd
rdquord` ta pramdss tbd soci ojraok sacutoak okta
tbd gftdrofc a mbaomd+
7/30/2019 Rockwood(2011)_Materials Fabrication From Bombyx Mori Silk Fibroin_Nature Protocols
dkvorakgdkt fk` dfturds a tbd pramdssokh usd` ta prdpfrd tbdsoci gftdrofc+ ]oci `dhrf`ftoak os gd`oftd` jy pratdfsds# wotb tbdpdpto`ds hdkdrftd` gdtfjacozd` jy mdccs?+ J+ garo yfrks bfvd jddk
okmujftd` ok pratdfsd ROU up ta 6: wddis# fk` ot bfs jddk sbawktbft wotb okmrdfsokh okmujftoak togd tbd dkzygd mcdfvd` tbd soci pratdok ft guctopcd camftoaks fcakh tbd mbfoks9 avdrfcc dkzygftom
`dhrf`ftoak wfs gd`oftd` jy surfmd drasoak6>+ Ok f``otoak# tbd`dhrf`ftoak rftd a soci mfk jd fctdrd` jy tbd ga`d a pramdssokh tbdojraok# fs wdcc fs past'pramdssokh trdftgdkts# rdcftd` ta tbd maktdkt
a κ'sbddt mrystfcs fk` dhrdd a arhfkozftoak a tbd kakmrystfccokd`agfoks+ Ok hdkdrfc# tbd dhrf`ftoak rftd dmrdfsds wotb fk okmrdfsdok avdrfcc κ'sbddt maktdkt+ Bawdvdr# ot bfs fcsa jddk sbawk tbft
tbd rftd a `dhrf`ftoak a soci joagftdrofcs `ordmtcy fdmtd` tbd
ok wbomb soci wfs sbawk ta bfvd cdvdcs a tugar kdmrasos fmtar'ζ# f mytaiokd ok`omf'tovd a fk okcfggftary rdspaksd# sogocfrta tbasd a tossud mucturd pcfstom6=+ Ok f``o'
toak# soci ojraok bfs jddk usd` ok sdvdrfcok vova stu`ods ar jrfok60# sat tossud64# suj'mutfkdaus65 fk` jakd68 fppcomftoaks+
frd okmcu`d` ok tbd ‒]oci ojraok dxtrfmtoak‟ pratamac+ Pbd dk` rdsuctos fk fqudaus sacutoak a purd soci ojraok tbft mfk jd usd` ok gfky a tbd pratamacs+ Fctdrkftovdcy# tbd sacutoak mfk jd cyapbocozd` ar
cakh'tdrg starfhd ar usd` ta pra`umd gftdrofcs ok arhfkom sacvdkt"6#6#6#=#=#='bdxfcuara':'prapfkac# BEO^/+
Akmd tbd dxtrfmtd` soci ojraok os pramdssd` okta akd a tbd sdvdrfc
argfts dsmrojd` bdrd# tbd okfc stdp os atdk ta ok`umd mrystfccokoty+Mrystfccokoty mfk jd ok`umd` vof twa gdtba`s# dotbdr jy oggdrsoakok fk fcmabac sumb fs gdtbfkac ar dtbfkac ar jy wftdr fkkdfc'
okh+ Fcmabac oggdrsoak os sogpcd fk` quomi jut o tbd rdsdfrmbdrwfkts ta fvao` tbd usd a fk fcmabac# wftdr fkkdfcokh mfk jd usd`+Xftdr fkkdfcokh os tbd pramdss ok wbomb tbd soci gftdrofcs frd okmu'
jftd` ok f bugo` dkvorakgdkt ar sdvdrfc baurs+ Ear gard dtfocd`
okargftoak# wd rddr tbd rdf`dr ta tbd stu`y jy Bu dt fc+62# wbomb
`dsmrojds f systdgftom fkfcysos a mrystfccokd ok`umtoak tbrauhbwftdr fkkdfcokh+
]oci ojraok dxtrfmtoak
Pbos pratamac os tbd stfrtokh paokt ok ar`dr ta ajtfok fky a tbdgftdrofc typds costd` dfrcodr‐spakhds# ocgs# ojdrs fk` hdcs+ Aurpratamac os `dsohkd` ta pra`umd akd jftmb rag 4 h a soci mamaaks9
bawdvdr# o gard gftdrofc os rdquord`# tbd vacugds mfk jd smfcd`fppraproftdcy "Eoh+ :/+ Akcy mamaaks tbft caai uk`fgfhd` sbauc`jd usd` ok tbd pramdss+ O kdmdssfry# soci mamaaks mfk jd rdpcfmd` wotb
jfvd ojdrs "rfw soci tdxtocd yfrk/ ok ]tdp 4# fk` tbd rdgfok`dr a tbd
sbawk ta `dhrf`d tbd gacdmucfr wdohbta tbd soci ojraok# fk` tbdrdard orrf`of'toak `asokh ar stdfg stdrocozftoak togokhsbauc` jd warid` aut ar dfmb systdg+ Ok
sagd mfsds# ot os sogpcdr ta stdrocozd tbdokfc pra`umt jdard usd+ Pbd prddrrd`gdtba`s a stdrocozftoak ar dfmb gftdrofc
argft frd katd` wotbok dfmb pratamac+^urood` soci mfk tbdk jd usd` ar f vfrodty a fppcomftoaks# fssuggfrozd` ok Eohurds =”66+
]oci tujds "]tdp :4# aptoaks F fk` J/
Pbdrd frd twa gdtba`s ar prdpfrokh soci tujds+ O stdrocozd` tujds
frd rdquord`# okmujftd tbd tujds ok f sacutoak a 8>$ "vac!vac/ dtbf'kac avdrkohbt fk` tbdk roksd tbdg ok stdrocd wftdr jdard usd+
Mdccs mfk jd f``d` ftdr tbd sakomftoak stdp fk` proar ta tbd hdcf'toak ta pravo`d bagahdkdaus fk` ddmtovd dkmfpsucftoak ok tbd
hdc+ Fs fk dxfgpcd# wd bfvd dkmfpsucftd` mdccs okta sakomftd` hdcsjy futamcfvokh 0$ "wt!vac/ soci fk` sakomftokh 4 gc a ot usokh tbdgdtba`s jdcaw+ ]dpfrftdcy# bugfk gdsdkmbygfc stdg mdccs wdrd
bfrvdstd` fk` jrauhbt up ta f makmdktrftoak a 4> Ø 6>5 mdccs pdrgc+ F vacugd a 4> ´c a tbd mdcc suspdksoak wfs f``d` ta tbd sako'mftd` soci fk` tbdk okmujftd` ft =8 ¾M fk` 4$ MA
:ar : b jdard
f``okh mdcc mucturd gd`oug:8+
Dcdmtrom murrdkt+ Pbd gftdrofc pra`umd` ok tbos pratamac os fk
f`bdsovd hdc:2+ Pbos hdc os rdvdrsojcd o tbd pacfroty a tbd vactfhd osswotmbd`+ Pbus# o f pasotovd vactfhd os fppcod` ta tbd dcdmtra`d tbftbf` f kdhftovd vactfhd prdvoauscy# tbd hdc wocc jdhok ta arg ak tbd
kdwcy pasotovd dcdmtra`d fk` tbd hdc tbft bf` argd` dfrcodr woccjdhok ta `osfssdgjcd+ Jdmfusd a tbd rdvdrsojcd kfturd a tbos hdc#ot mfk jd usd` ok fppcomftoaks ok wbomb ot os f`vfktfhdaus ta bfvdtukfjcd f`bdsoak+
]oci ocgs "]tdp :4# aptoaks H fk` B/
Eocgs pravo`d f wfy ta smrddk gftdrofcs ar ok votra ar ok vova tdst'
smfac`s# by`rahdcs ar ocgs+Pbd sdmak` pratamac# wbomb utocozds ^UF# wocc hdkdrftd soci spbdrds wotb f sozd rfkhd jdtwddk =>> kg fk` :> ´g=0+ Pbd sozd
a tbd spbdrds mfk jd maktraccd` jy mbfkhokh tbd makmdktrftoak a tbd soci ar ^UF sacutoaks ar jy sakomftokh ta tbd sacutoak proar tamfstokh tbd ocg+ Pa mbfrfmtdrozd tbdsd spbdrds# f dw tdmbkoquds
bfvd jddk usd`# okmcu`okh ykfgom cohbt smfttdrokh ta dtdrgokd tbdsozd a tbd kfkagdtdr spbdrds# cfsdr cohbt `orfmtoak ta `dtdrgokdtbd sozd a tbd gomraspbdrds fk` ]DG ta ogfhd tbd garpbacahy+
8| ^cfmd jraok ok f 6'cotdr jdfidr ccd` wotb 6 cotdr a uctrfpurd wftdr fk` f stor jfr+& mfutOak O yau frd usokh f pcfstom jdfidr# dksurd tbft tbd bat pcftd bfs maacd`+
2| Zoksd tbd jraok ok wftdr ar :> gok wbocd hdktcy storrokh ak f stor pcftd+
?| Zdpdft ]tdps 8 fk` 2 twomd ar f tatfc a tbrdd roksds+
6>| Ftdr tbd tbor` wfsb# rdgavd tbd soci# squddzd ot wdcc fk` tbdk sprdf` ot aut ak f mcdfk podmd a fcugokug aoc+
66| Fccaw tbd soci jraok ta `ry ok f ugd baa` avdrkohbt+
pfusd paOkt @dhuggd` soci ojraok# ok wbomb tbd sdromok bfs jddk rdgavd`# mfk jd stard` ok`dokotdcy ft raagtdgpdrfturd+ Ear cakh'tdrg starfhd# pcfmd ot ok f mcdfk pcfstom jfh ar wrfp ot ok fcugokug aoc+ Jd surd ta ok`omftd tbd cdkhtba tbd jaocokh stdp ak tbd cfjdc+
@ov oi ojo o coJ ◁ tOGOkH 0+4 b "`y :/6:| Mfcmucftd tbd fgaukt a ?+= G cotboug jrago`d kdd`d` ta prdpfrd f :>$ "wt!vac/ sacutoak jfsd` ak tbd fgaukt a `rod`
jraok fvfocfjcd+ Fs :>$ a tbd sacutoak wocc jd soci# 2>$ wocc jd CoJr+ Pbft os# f rftoa a 6<0 "6 h ta 0 gc/ soci ta CoJr+ Pbdrd'ard# guctopcy tbd fgaukt a tbd `rod` soci jraok jy 0 ta ajtfok tbd tatfc vacugd a ?+= G CoJr kdd`d` " R /+
6=| ^rdpfrd f ?+= G CoJr sacutoak+
25 24 ? =6
6 >>>+ +
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7/30/2019 Rockwood(2011)_Materials Fabrication From Bombyx Mori Silk Fibroin_Nature Protocols
& mfutOak F``okh CoJr ta wftdr rdsucts ok fk dxatbdrgom rdfmtoak9 jd gok`uc a tbd bdft hdkdrftd`+ Xbdk prdpfrokh cfrhdvacugds# wd rdmaggdk` mfrryokh tbos aut ak omd+
mrOtOmfc stdp CoJr bfs f caw `dksoty fk` ots vacugd sbauc` jd tfidk okta fmmaukt wbocd prdpfrokh tbd sacutoak+ Xdsuhhdst f``okh akcy 5>$ a tbd mfcmucftd` vacugd a wftdr fk` tbdk jrokhokh tbd sacutoak up ta tbd kfc vacugd+ ]tor wotb f
sgfcc stor jfr+
60| ^fmi soci jraok tohbtcy okta f 4>'gc hcfss jdfidr fk` f`` tbd rdquord` fgaukt a CoJr sacutoak ak tap+ mrOtOmfc stdp Pbd CoJr gust jd f``d` ta tbd soci rftbdr tbfk f``okh soci ta tbd CoJr sa tbft tbd soci wocc dvdktufccy jdmavdrd` fk` `ossacvd` jy tbd CoJr+ Ot os fcsa bdcpuc ta usd tbd sgfccdst hcfss maktfokdr tbft wocc stocc bac` tbd soci fk` CoJrsacutoak+
64| Cdt jraok `ossacvd ok fk avdk ft 5> ¾M ar 0 b+ Akmd tbd soci jraok os magpcdtdcy `ossacvd`# ot wocc fppdfr fgjdr okmacar fk` wocc jd trfkspfrdkt+ Jcfmi jots rag tbd sociwarg gfy jd vosojcd jut wocc jd rdgavd` cftdr+ Pbos sacutoak wocc jd
bohbcy vosmaus jut sbauc` kat maktfok fky oktfmt jdrs# fs `dtdrgokd` jy vosufc fssdssgdkt+1 trauJcdsBaatOkH
@oyo ` oho ◁ tOGOkH 0? b "`y :”0/65| By`rftd `ofcysos mfssdttds ok wftdr ar f dw gokutds+
68| Xotb f :>'gc syrokhd fk` fk 62'hfuhd kdd`cd# oksdrt 6: gc a tbd soci'CoJr sacutoak okta f =”6:'gc `ofcysos mfssdttd+
mrOtOmfc stdp Jd mfrduc kat ta pukmturd ar taumb tbd `ofcysos gdgjrfkd+ Pbd sacutoak wocc jd vdry vosmaus# fk` tbosstdp wocc jd dfsodr o tbd sacutoak os idpt wfrg jdard f``okh ta tbd mfssdttd+ Ot os ogpartfkt ta fvao` sbdfrokh tbd sacu'
toak wbdkdvdr passojcd ta fvao` tbd ok`umtoak a κ'sbddt wotbok tbd soci+ Pbdrdard# akcy usd tbd kdd`cd wbdk okldmtokh oktatbd mfssdttd+ Gardavdr# bfvd fk f``otoakfc kdd`cd fk` oksdrt ot okta fkatbdr tap part a tbd mfssdttd ta fccaw for ta dsmfpd+Zdgavd tbd dxtrf kdd`cd akmd fcc tbd for bfs jddk purhd`+
62| @ofcyzd fhfokst 6 cotdr a uctrfpurd wftdr pdr 6: gc mfssdttd+ Pa dksurd goxokh# usd f cfrhd stor jfr fk` pcfmd ak f gfh'kdtom stor pcftd+ Mbfkhd tbd wftdr ftdr 6 b# 0 b# tbft dvdkokh# tbd kdxt garkokh fk` kohbt# fs wdcc fs ok tbd garkokh ak tbdaccawokh `fy "o+d+# sox mbfkhds wotbok 02 b/+
6?| Zdgavd soci rag tbd mfssdttds wotb fkatbdr :>'gc syrokhd fk` fk 62'hfuhd kdd`cd+ ^cfmd soci ok f 4>'gc makomfc tujd+ @dpdk`'okh ak tbd vacugd# dotbdr spcot ot jdtwddk twa tujds "o gard tbfk 0> gc/ ar cc akd tujd fk` usd f mauktdrjfcfkmd a wftdr+
:>| Mdktrouhd ta rdgavd ogpurotods+ ^cfmd ok f mdktrouhd fk` spok ft ?#>>> r+p+g+ "~6:#8>> h / ft 0 ¾M ar :> gok+
:6| Mfrduccy rdgavd tujds rag tbd mdktrouhd fk` dotbdr paur ar trfksdr tbd soci sacutoak wotb f :4 gc popdttd okta fkatbdrmdktrouhd tujd+ Jd surd ta cdfvd fky wbotd eammucdkt ar jrawk gfttdr jdbok`+
::| Zdpdft ]tdps :> fk` :6 fhfok+
:=| Pa `dtdrgokd tbd makmdktrftoak a tbd soci ok sacutoak# gdfsurd tbd wdohbt a f sgfcc wdohb jaft+ Pbdrdftdr# f`` >+4 gc a tbd soci sacutoak ta tbd jaft fk` fccaw ot ta `ry ft 5> ¾M+ Akmd tbd soci os `ry# `dtdrgokd tbd wdohbt a tbd soci fk` `ovo`dot jy >+4 gc+ Pbos wocc yodc` tbd wdohbt pdr vacugd pdrmdktfhd+
mrOtOmfc stdp F jftmb a 4 h a soci mamaaks hdkdrfccy yodc`s :4 gc a 8”2$ "wt!vac/ soci sacutoak+ Pbd sacutoak wocc jdtoktd` ydccaw jut sbauc` jd rdcftovdcy mcdfr fk` scohbtcy gard vosmaus tbfk wftdr+ O tbdrd frd ogpurotods sumb fs wbotd eam'mucdkts ar `fri pfrtomucftds# ot os jdst ta rdmdktrouhd ta rdgavd tbdg+
pfusd paOkt Pbd soci sacutoak mfk jd stard` ft 0 ¾M ar ft cdfst f gaktb+ @dpdk`okh ak tbd puroty# stard` soci wocc dvdktufccy hdc jut hdcftoak togds wocc vfry+ Akmd tbd soci bfs hdccd`# ot mfkkat jd usd` ar pratamacs tbft rdquord sacutoak fk`tbdrdard fkatbdr jftmb wocc kdd` ta jd dxtrfmtd`+
cyboozo ` o:0| Pbd jraok sacutoak ":4 gc ft makmdktrftoak 8”2$ "wt!vac// mfk dotbdr jd usd` fs os ar ot mfk jd cyapbocozd` "aptoak F/ar makmdktrftd` "aptoak J/+ Ear starfhd ar cakhdr tbfk 6 gaktb# tbd soci sacutoak sbauc` jd cyapbocozd`+ Ok tbos arg#tbd soci wocc jd stfjcd ar ydfrs ft raag tdgpdrfturd fk` mfk jd rdmakstotutd` ok BEO^+ Pbd makmdktrftd` sacutoak ":>”=>$#wt!vac/ mfk jd usd` `ordmtcy ar prdpfrokh soci tujds+
7/30/2019 Rockwood(2011)_Materials Fabrication From Bombyx Mori Silk Fibroin_Nature Protocols
"f/ cyboozo "o/ ◁ tOGOkH = `"o/ @ovo`d tbd fqudaus soci sacutoak okta 4>'gc makomfc tujds wotb ka gard tbfk :> gc pdr tujd+"oo/ ^cfmd vdrtomfccy ok f √ 2> ¾M rddzdr ar sdvdrfc baurs uktoc tbd sacutoak os magpcdtdcy razdk+ O f √ 2> ¾M rddzdr os kat
fvfocfjcd# soci mfk fcsa jd razdk ft √ :> ¾M avdrkohbt ar pcfmd` ok coquo` kotrahdk uktoc tbd sacutoak os razdk+"ooo/ Eac` f Iogwopd avdr tbd tap a tbd tujd fk` fttfmb wotb dotbdr f rujjdr jfk` ar tfpd+ Iddp mfps ar cftdr usd+
"ov/ ^cfmd razdk sfgpcds ak f cyapbocozdr ar :”= ` uktoc fcc a tbd wftdr os rdgavd` rag tbd sacutoak+ Pbd tujd wocc kacakhdr ddc mac`+
"J/ moh oi ojo "o/ ◁ tOGOkH :6”:4 b"o/ F`` 6>> h a ^DH ta ?>> gc a uctrfpurd wftdr ta prdpfrd f 6>$ "wt!vac/ ^DH sacutoak+ Gox wotb f cfrhd stor jfr ak f
gfhkdtom stor pcftd+ Pbd sacutoak sbauc` jd mcdfr akmd tbd ^DH os magpcdtdcy `ossacvd`+ Xbocd wfotokh ar tbd ^DH ta`ossacvd# gavd ta ]tdp :0J"oo/+
"oo/ By`rftd `ofcysos mfssdttd ok f 6>>'gc jdfidr ccd` wotb uctrfpurd wftdr ar f dw gokutds+
"ooo/ Zdgavd tbd mfssdttd rag tbd wftdr fk` tfp tbd jattag a tbd mfssdttd ak f pfpdr tawdc ta `ry+ Jd surd ta bac` tbdmfssdttd ft tbd d`hds fk` `a kat taumb tbd gdgjrfkd+
"ov/ @rfw aut 6> gc a 8”2$ "wt!vac/ soci sacutoak wotb tbd 6>'gc syrokhd wotbaut f kdd`cd+"v/ Fttfmb tbd 62'hfuhd kdd`cd ta tbd syrokhd ccd` wotb jraok sacutoak+"vo/ Oksdrt tbd top a tbd kdd`cd okta f tap part a tbd mfssdttd+
mrOtOmfc stdp Jd mfrduc ta oksdrt lust tbd top a tbd kdd`cd ta fvao` pukmturokh tbd gdgjrfkd+"voo/ ]cawcy oksdrt tbd jraok sacutoak okta tbd mfssdttd+
mrOtOmfc stdp Fvao` bohb sbdfr tbft gfy fccaw prdgfturd fcohkgdkt a tbd κ'sbddts ok tbd soci jraok+"vooo/ Fs tbd mfssdttd jdmagds okeftd`# purhd dxmdss for jy oksdrtokh fkatbdr 62'hfuhd kdd`cd okta tbd atbdr tap part ta
fccaw tbd for ta dsmfpd+"ox/ Ftdr for os rdgavd`# wotb`rfw tbd dxtrf kdd`cd rag tbd mfssdttd+"x/ Eokosb ccokh tbd mfssdttd wotb tbd rdgfokokh jraok sacutoak+ Fctbauhb tbd mfssdttd ok`omftds f gfxogug vacugd a
makmdktrftd` ar :> b+ Ear tbd hdc'spuk tujds# tbd sacutoak gust jd gard vosmaus fk` tbdrdard os makmdktrftd` ar ~:: b+"xv/ Zdgavd tbd makmdktrftd` soci sacutoak ftdr tbd `dsord` fgaukt a togd usokh f ='gc syrokhd fk` fk 62'hfuhd kdd`cd+"xvo/ Gdfsurd tbd sacutoak makmdktrftoak jy wdohbokh aut f sgfcc wdohb jaft fk` tbdk f``okh >+6 gc a makmdktrftd` soci
sacutoak ta tbd jaft+ Jdmfusd a tbd bohb vosmasoty a makmdktrftd` soci# wd suhhdst usokh dotbdr cfrhd'aromd popdttdtops ar muttokh a tbd top a tbd popdttd+ @ry tbd sacutoak ft 5> ¾M fk` tbdk wdohb tbd `rod` cg+ Pbd wdohbt a tbd soci`ovo`d` jy tbd vacugd usd` ">+6 gc/ wocc yodc` tbd wdohbt pdr vacugd pdrmdkt+ mrOtOmfc stdp @dpdk`okh ak tbd okotofc soci makmdktrftoak# 6> gc a soci jraok wocc yodc` :”0 gc a makmdktrftd` socisacutoak ftdr `ofcyzokh fhfokst 6>$ "wt!vac/ ^DH ar :>”:: b+ Pbd fgaukt a togd usd` ta makmdktrftd tbd sacutoakgfy kdd` ta jd fctdrd` ar dfmb jftmb+ Pbd makmdktrftoak togd os kat cokdfr# sa mfrd gust jd tfidk ta fvao` hdccokh tbdsoci ok tbd mfssdttd o ot os cdt ok tbd ^DH sacutoak ar taa cakh+ Xotb dxpdrodkmd# tbd rdsdfrmbdr wocc jd fjcd ta vosufccy
`dtdrgokd wbdtbdr tbd soci os makmdktrftd` dkauhb+ O tbd soci os maccdmtd` taa dfrcy# ot mfk dotbdr jd rdpcfmd` okta fmfssdttd fk` fccaw tbd `ofcysos ta pramdd` ar gard togd ar ot mfk jd stard` ft 0 ¾M+ Xd k` tbft starokh tbd soci ar f dw`fys wocc fccaw ot ta rdfmb tbd rdquord` makmdktrftoak+ Ot os suhhdstd` tbft tbd soci jd mbdmid` `focy# fs tbd sacutoak mfk
hdc ftdr ot bfs jddk stard` ar taa cakh "fppraxogftdcy 6”: wddis/+ Pbd sacutoak mfk jd mbdmid` jy fttdgptokh ta usdot ok tbd oktdk`d` pratamac+ Ear dxfgpcd# ar `oppd` tujds# f gfk`rdc mfk jd `oppd` okta tbd sacutoak fk` tbdk ot mfk jdvosufccy `dtdrgokd` wbdtbdr guctopcd jdf`s arg wotbaut mafcdsmokh+ O tbos ammurs# tbdk tbd sacutoak os kat rdf`y+ Pbdrdsuctokh soci sacutoak sbauc` jd vdry vosmaus fk` wocc fppdfr scohbtcy mcau`y wbdk magpfrd` wotb tbd stfrtokh sacutoak+
mrOtOmfc stdp Jd mfrduc kat ta oktra`umd jujjcds ta tbd sacutoak# fs tbdy frd vdry `omuct ta rdgavd cftdr+
pfusd paOkt Cfjdc fk` stard ft 0 ¾M ar f dw `fys+ Makmdktrftd` soci mfk akcy jd stard` ar f dw `fys# passojcyup ta f wddi# jdard ot hdcs+ Akcy makmdktrftd soci wbdk ot os oktdk`d` ta jd usd` rdcftovdcy saak+
:4| Pbd soci jraok mfk kaw jd usd` ta prdpfrd f kugjdr a `odrdkt gftdrofcs "Eoh+ 6/+ Tsokh tbd makmdktrftd` sacutoak"prdpfrd` ok ]tdp :0J/ yau mfk prdpfrd soci tujds jy dotbdr f sogpcd `op gdtba` ta mrdftd tbok'wfccd` tujds "aptoak F# Eoh+ =/ arjy hdc spokkokh# wbdrdok tbd jraok os dxtru`d` akta f ratftokh gfk`rdc "aptoak J# Eoh+ 0/+ Fctdrkftovdcy# soci sacutoak gfy jd usd`
7/30/2019 Rockwood(2011)_Materials Fabrication From Bombyx Mori Silk Fibroin_Nature Protocols
ta prdpfrd by`rahdcs vof vartdxokh# sakomftoak# dcdmtromfc murrdkt ar pB mbfkhd "aptoaks M”E# Eoh+ 4/9 kakpfttdrkd` ar pfttdrkd` socicgs "aptoaks H fk` B# Eoh+ 5/9 soci gomraspbdrds usokh tbd @A^M "aptoak O# Eoh+ 8/ ar UF "aptoak L# Eoh+ 2/ gdtba`9 dcdmtraspuksoci jdrs "aptoak I# Eoh+ ?/9 ar soci spakhds tbft frd dotbdr fqudaus'jfsd` "aptoak C# Eoh+ 6>/ ar BEO^'jfsd` "aptoak G# Eoh+ 66/+
"f/ @o` j ◁ tOGOkH : go j ob vohb `y ` 6 b i"o/ "Aptoakfc/ O paraus tujds frd rdquord`# prdpfrd f sacutoak a 8”2$ "wt!vac/ ^DA fk` mfrduccy f`` tbd fppraproftd
fgaukt ta tbd makmdktrftd` soci sacutoak+ Hdktcy stor wotb f stfokcdss stddc word+ mrOtOmfc stdp Ot os ogpartfkt kat ta oktra`umd jujjcds ta dotbdr sacutoak fs jatb frd bohbcy vosmaus fk` jujjcdsfrd `omuct ta rdgavd+ O jujjcds frd prdsdkt# tbdy wocc mrdftd bacds ok tbd rdsuctokh soci tujds+
"oo/ @op stfokcdss stddc word okta makmdktrftd` soci sacutoak+ Zdgavd tbd word rag tbd soci fk` okvdrt tbd word# fccawokh tbdjdf`s a soci ta dvdkcy ruk `awk tbd word+Xbdk tbd jdf`s mafcdsmd ft tbd jattag a tbd word# eop ot fk` fccaw tbd soci ta ruk jfmi `awk tawfr` tbd dk` a tbdword "try ta maft dvdkcy/+
1 trauJcdsBaatOkH"ooo/ Cdt dxmdss soci jdf`s `rop a a tbd word jfmi okta soci sacutoak+
"ov/ ^cfmd tbd words ok f vofc a gdtbfkac ar 4”6> s+ mrOtOmfc stdp Zdgavd tbd soci'maftd` word rag gdtbfkac fk` fccaw ta `ry ar ~=> s+
"v/ Zdpdft `oppokh pramdss fk` gdtbfkac trdftgdkt :”0 togds ar uktoc tbd word os sumodktcy maftd` wotb soci+ Pbd socigfy fppdfr ukdvdk# jut ot wocc sbroki fs ot `rods+
"vo/ @ry tujds jy stomiokh tbd okvdrtd` soci'maftd` word okta styraafg fk` pcfmd ot ok f ugd baa` avdrkohbt+ Xd suhhdst
gfiokh fk dxmdss kugjdr a tujds# fs sagd a tbd tujds gfy mrfmi `urokh tbd `ryokh pramdss+ pfusd paOkt @rod` tujds mfk jd stard` ar sdvdrfc `fys ft raag tdgpdrfturd+
"voo/ Xbdk soci os `ry# pcfmd tbd words maftd` wotb soci okta f 64'gc makomfc tujd ccd` wotb safpy wftdr fk` safi ar 6 b+"vooo/ Ftdr safiokh# rdgavd tbd tujds rag tbd safpy wftdr fk` mut tbd dk` a tbd soci tujd wotb f smfcpdc ar f rfzar jcf`d+
"ox/ Zdgavd tbd tujd rag tbd word usokh twddzdrs+ Pbd tujd sbauc` sco`d a dfsocy+ O ot os `omuct ta rdgavd wotbautsmrukmbokh tbd tujd# tbdk pcfmd tbd tujd jfmi okta safpy wftdr fk` wfot jdard tryokh fhfok+1 trauJcdsBaatOkH
"x/ Eocc f pcfstom ^dtro `osb "6> mg/ wotb uctrfpurd wftdr+ ^cfmd tbd tujds ok tbd wftdr fk` okmujftd ak f sbfiokh wftdrjftb ar 6 b ta rdgavd rdso ufc safp+
"xo/ ]tard soci tujds ok f pcfstom ^dtro `osb ft raag tdgpdrfturd+ Pbdsd tujds mfk jd stard` ar sdvdrfc gaktbs up ta fmaupcd a ydfrs+ Pbdy frd jrottcd# sa wd suhhdst tbft tbdy jd by`rftd` ok wftdr jdard jdokh gfkopucftd`+
"J/ H' j ◁ tOGOkH 4 go j vohb `y
"o/ ^cfmd tbd gfk`rdc ok tbd gfk`rdc ratftoak systdg+"oo/ Fttfmb f 60'hfuhd kdd`cd ak f 6'gc syrokhd fk` scawcy `rfw up tbd makmdktrftd` soci sacutoak+ Jd surd ta fvao`okmcu`okh for jujjcds+
"ooo/ Zdgavd tbd kdd`cd fk` rdpcfmd wotb f =>'hfuhd kdd`cd+"ov/ ]tfrt tbd gfk`rdc ratftoak systdg+"v/ ]quddzd tbd hdc sacutoak aut a tbd kdd`cd fk` akta tbd gfk`rdc+ Pbd jdr sbauc` jd ukoarg wotbaut fky jdf`s ar
`osmaktokuotods+ Pa ajtfok f ukoarg jdr# f hdkdraus fgaukt a prdssurd os rdquord`+ O sacutoak `ads kat magd aut#rdpcfmd tbd kdd`cd wotb f :8'hfuhd kdd`cd+
mrOtOmfc stdp Pbd wok`okh pfttdrk mfk jd fctdrd` tbrauhb tbd usd a tbd gfk`rdc ratftoak systdg+ O tbdratftoak os makstfkt fk` tbd gfk`rdc asmoccftds jfmi fk` artb# f mrass'bftmbd` `dsohk mfk jd hdkdrftd`+ O f jdr
rukkokh ok tbd sfgd `ordmtoak os rdquord`# tbd gfk`rdc ratftoak systdg sbauc` jd stappd` ftdr akd cfydr fk`tbdk tbd ratftoak rdvdrsd`+ Pbd wfcc tbomikdss ajtfokd` wotb tbos gdtba` wocc `dpdk` ak tbd kugjdr a cfydrs fk`tbd spdd` a tbd trfksvdrsd gatar+
1 trauJcdsBaatOkH"vo/ Akmd tbd soci bfs jddk cfo` akta tbd gfk`rdc# fppcy gdtbfkac ak tap a tbd soci wotb f kdd`cd fk` syrokhd ok ar`dr ta
ok`umd κ'sbddt argftoak wotbok tbd soci jraok+"voo/ Fccaw tbd gdtbfkac ta `ry# fk` tbdk pcfmd tbd gfk`rdc okta f sacutoak a safpy wftdr+"vooo/ Xbdk tbd tujd bfs satdkd`# hrfj tbd tujd ukoargcy wotb f Iogwopd fk` hdktcy pucc ot a+ O tbd tujd `ads kat
rdf`ocy gavd# maktokud ta safi ot ok tbd safp sacutoak+
"ox/ Eocc f pcfstom ^dtro `osb "6> mg/ wotb uctrfpurd wftdr+ ^cfmd tbd tujd ok wftdr fk` okmujftd ok f sbfiokh wftdr jftb ar6 b ta rdgavd rdso`ufc safp+
"x/ ]tard tbd soci tujd ok f pcfstom ^dtro `osb ft raag tdgpdrfturd ar up ta 6 ydfr "`dpdk`okh ak tujd trdftgdkt fk`starfhd mak`otoaks/+
"m/ Ux` h ◁ tOGOkH 6 `"o/ F`lust tbd makmdktrftoak a fk 2$ "wt!vac/ soci sacutoak ta :”4$ "wt!vac/+
7/30/2019 Rockwood(2011)_Materials Fabrication From Bombyx Mori Silk Fibroin_Nature Protocols
"oo/ Prfksdr 6 gc a soci sacutoak okta f hcfss vofc fk` mcasd tohbtcy+ mrOtOmfc stdp Pbos pratamac os ar 6 gc a caw'makmdktrftoak soci sacutoak+ Cfrhdr vacugds gfy jd usd`# jut tbdvartdx togd fk` spdd` gfy kdd` ta jd fctdrd`+ Ot os kat kdmdssfry ta usd hcfss vofcs+
"ooo/ ]dmurd tbd vofc ok fk uprohbt pasotoak ak tbd vartdxdr wotb `umt tfpd+"ov/ Uartdx tbd sacutoak ok tbd hcfss vofc ar ~8 gok ft =#:>> r+p+g+ "gfxogug spdd` sdttokh/+ Pbos trdftgdkt sbauc`
"v/ Maccdmt tbd turjo` sacutoak usokh f 6'gc scop'top syrokhd fvao`okh fky passojcd saco`# stomiy pbfsd+"vo/ Okmujftd turjo` sacutoak avdrkohbt ok tbd syrokhd ar ok f wdcc pcftd ta fccaw hdcftoak+ Okmujftoak ft =8 ¾M wocc `dmrdfsd
tbd hdcftoak togd+ Ear cakh'tdrg starfhd# stard ft 0 ¾M+"voo/ Hdcs mfk jd okldmtd` tbrauhb :6'hfuhd "ar cfrhdr `ofgdtdr/ kdd`cds o kdmdssfry+ Gokogfc armd sbauc` jd sumodkt ta
okldmt f 4$ "wt!vac/ hdc tbrauhb f :6'hfuhd kdd`cd+ O okldmtoak rdquords sujstfktofc armd# tbd top a tbd syrokhd mfk
jd mut usokh f jcf`d "jdard makkdmtokh tbd kdd`cd/ ta rdgavd tbd siok cfydr ft tbd top+
"@/ so` h ◁ tOGOkH 6 `"o/ @ocutd 2$ "wt!vac/ fqudaus soci sacutoak ta 0$ "wt!vac/+"oo/ F`` 4 gc a 0$ "wt!vac/ soci sacutoak ta f 64'gc makomfc tujd+"ooo/ ]akomftd tbd sacutoak ft 4>$ fgpcotu`d ":6 X/ ar => s+
& mfutOak Pbd sakomftokh bark gust jd oggdrsd` ok sacutoak wbocd tbd pawdr os fppcod` ok ar`dr ta prdvdkt `fgfhdta tbd okstrugdkt+ Jd mfrduc kat ta taumb tbd bark ak tbd so`ds a tbd tujd `urokh sakomftoak+
& mfutOak Dxtdk`d` dxpasurd ta uctrfsakomftoak gfy mfusd `fgfhd ta akd‟s bdfrokh+ Tsd a pratdmtovd dfrgus osbohbcy rdmaggdk`d +
1 trauJcdsBaatOkH"ov/ O rdquord`# tbd turjo` sacutoak mfk jd popdttd` okta f wdcc pcftd ar f ^dtro `osb oggd`oftdcy ftdr sakomftoak# proar ta hdcftoak+"v/ Okmujftd tbd sakomftd` sacutoaks ft =8 ¾M avdrkohbt# o kdmdssfry# ta fccaw by`rahdcftoak+
"d/ dh "'h/ ◁ tOGOkH 64 go"o/ F`` 6> gc a 8”2$ "wt!vac/ fqudaus soci sacutoak ta f 4>'gc makomfc tujd+"oo/ Oggdrsd f pfor a pcftokug word dcdmtra`ds okta tbd soci sacutoak+ O tbd dcdmtra`ds `a kat rdfmb tbd sacutoak# mut a
tbd tap partoak a tbd tujd+"ooo/ Fppcy ~:4 U
@Mta tbd pcftokug dcdmtra`ds avdr f dw gokutds+ Xotbok sdmak`s a tbd fppcomftoak a tbd vactfhd# f
vosojcd hdc wocc arg ft tbd pasotovd dcdmtra`d# camiokh ok sagd axyhdk jujjcds ft tbd dcdmtra`d surfmd fs tbd hdc dgfkftds autwfr`+1 trauJcdsBaatOkH
"ov/ Purk a tbd pawdr suppcy wbdk f``otoakfc hdc ka cakhdr maccdmts ak tbd pasotovd dcdmtra`d+"v/ Maccdmt tbd hdc ak tbd pasotovd dcdmtra`d+"vo/ "Aptoakfc/ O f stodr hdc os rdquord`# maccdmt tbd d'hdc okta f syrokhd jy rdgavokh tbd pcukhdr fk` pcfmokh tbd soci ok
tbd apdk dk`+ Pbd κ'sbddt maktdkt a tbd soci os okmrdfsd` wbdk f rfpo` sbdfr armd os fppcod` ta tbd hdc+
"E/ B h ◁ tOGOkH 6> go"o/ F`` >+? gc a 2$ "wt!vac/ fqudaus soci sacutoak ta f hcfss vofc+ Pbos pratamac bfs jddk dstfjcosbd` ar f tatfc sacutoak
vacugd a 6 gc# jut tbd vacugds mfk jd smfcd` up o tbd vacugdtrom rftoa a soci ta fmo` os gfoktfokd` ft 6><6+
"oo/ F`` >+6 gc a >+= G BMc ta tbd vofc+ Pbd gacfroty a tbd BMc mfk jd okmrdfsd` ar `dmrdfsd` `dpdk`okh ak tbd kdd`s a tbd usdr+ Xd bfvd stu`od` hdcs argd` wotb >+6# >+= fk` 6+> G BMc fk` bfvd auk` tbft tbd stokdss okmrdfsd` wotbokmrdfsokh fmo` makmdktrftoak+ Ok f``otoak# wd auk` tbft tbd >+= G BMc hdcs wdrd tbd gast f`bdsovd+
"ooo/ Mcasd mfp fk` stor jy okvdrtokh tbd vofc+"H/ soi eg ◁ tOGOkH : `
"voo/ ]tard ft raag tdgpdrfturd ar up ta 6 ydfr "`dpdk`okh ak cg trdftgdkt fk` starfhd mak`otoaks/+
"B/ p` oi eg ◁ tOGOkH 5 b hoh g`# :` oh"o/ ^rdpfrd wari frdf jy cfyokh `awk f sbddt a fcugokug aoc fk` maccdmtokh tbd rdquord` gftdrofcs "f pcfstom popdttd# f
jax a Iogwopds# f pfpdr mup# f kdd`cd# f ]ychfr` jfsd fk` murokh fhdkt# f jattcd a 8>$ "vac!vac/ dtbfkac fk``orfmtoak hrftokh/+ mrOtOmfc stdp O yau frd rdusokh f gac`# siop ta ]tdp :4B"xoo/+ mrOtOmfc stdp Dksurd tbft tbd `orfmtoak hrftokh os rdd a `djros+ ]prfy wotb dtbfkac# wopd fk` jcaw'`ry wotbprdssurozd` for rag tbd jdkmb tap+
"oo/ ^cfmd pfpdr mup ak jfcfkmd# wdohb aut fk fppraproftd fgaukt a jfsd fk` tbdk murokh fhdkt rag tbd ]ychfr` iot+Ear 6 gac` "4 mg Ø 4 mg/ usd 0+4 h a jfsd fk` >+4 h a murokh fhdkt "?<6 rftoa/+
"ooo/ Gox wotb f pcfstom popdttd ar 6 gok+"ov/ ^cfmd ^@G] ok f vfmuug avdk ar =>”5> gok ta rdgavd for jujjcds+
"v/ ^cfmd tbd `orfmtoak hrftokh ok f ^dtro `osb+"vo/ ]cawcy paur tbd ^@G] avdr tbd `orfmtoak hrftokh9 try kat ta oktra`umd jujjcds+"voo/ Akmd tbd hrftokh os mavdrd`# rdgavd fky jujjcds wotb f kdd`cd+"vooo/ Hdktcy gavd tbd ^dtro `osb ta f 5> ¾M avdk ar 0 b ar cdfvd ft raag tdgpdrfturd ar avdr :0 b+"ox/ Zdgavd tbd ^@G] rag tbd `orfmtoak hrftokh+ Jd mfrduc kat ta rop tbd ^@G]+
"xo/ "Aptoakfc/ tbd ^@G] gac` mfk jd pukmbd` ta vfryokh `ofgdtdrs `dpdk`okh ak kdd`+ Ear tbos pratamac# pukmb aut
60'gg `osis rag tbd ^@G]+"xoo/ ^cfmd tbd ^@G] gac`s ok f ^dtro `osb+"xooo/ F`` 6>> ´c a 2$ "wt!vac/ soci sacutoak akta dfmb 60'gg `osi+"xov/ Fccaw tbd cgs ta `ry avdrkohbt+"xv/ Eocc tbd jattag a tbd vfmuug `dsommftar wotb wftdr+
mrOtOmfc stdp ^cfmd tbd `ry cgs ok tbd `dsommftar fk` fppcy vfmuug ta tbd vfmuug part+"xvo/ Fccaw tbd cgs ta wftdr fkkdfc ar 6 `+"xvoo/ Fccaw tbd cgs ta `ry ar 6> gok fk` hdktcy pddc cgs rag tbd gac` usokh twddzdrs+
"xvooo/ Pa ok`omftd tbd pfttdrkd` so`d a tbd cg# pcfmd fk d`hd a tbd kakpfttdrkd` so`d akta f ^ast'ot katd+ ]tard ft raagtdgpdrfturd uktoc usd+
"O/ Gob "@apm/ ◁ tOGOkH 0+4 `"o/ F`` :>> gh a @A^M copo` paw`dr ta f hcfss tdst tujd+"oo/ Ok f baa`# f`` 6 gc a mbcaraarg fk` wfot uktoc tbd @A^M os magpcdtdcy `ossacvd`9 tbd sacutoak wocc turk mcdfr+
& mfutOak Mbcaraarg os f bfzfr`aus sujstfkmd ikawk ta jd mfrmokahdkom9 wdfr prapdr sfdty dquopgdkt fk` wariokso`d f mbdgomfc ugd baa`+
"ooo/ Zacc tbd tujd ta dvdkcy maft tbd oktdroar a tbd tujd wotb f tbok# bagahdkaus cfydr+ Xbocd raccokh tbd tujd# `ry autsacutoak wotb kotrahdk hfs eaw+ Xbdk tbd cg os kdfrcy `ry# tbd eaw rftd mfk jd okmrdfsd`+
"ov/ Cdfvd tbd tujd ta `ry ok tbd baa` ar => gok+
pfusd paOkt Hcfss tujds mfk jd maftd` wotb @A^M fk` stard` ft √ :> ¾M uktoc kdd`d`+"v/ Ok f 6+4'gc gomramdktrouhd tujd# f`` :>> ´c a 6> gh gc √ 6 sacutoak a tbd `ruh# pratdok ar mbdgomfc ta jd dkmfpsu'
cftd` ta 6 gc a 2$ "wt!vac/ soci sacutoak+ O `dsord`# fcsa prdpfrd f sacutoak a 6 gc a 2$ "wt!vac/ soci wotb :>> ´c a
uctrfpurd wftdr ar f maktrac sfgpcd+"vo/ By`rftd tbd copo` cg wotb tbd soci fk` `ruh sacutoak "ar maktrac sacutoak# o fppcomfjcd/+ Jy f``okh tbd fqudaus sacu'
"xo/ Zdpdft ]tdps ?”6> twomd gard ta magpcdtd tbrdd rddzd'tbfw mymcds "ok ar`dr ta mrdftd f gard bagahdkaus sozd`ostrojutoak a spbdrds/+
"xoo/ Eocc f 6>>'gc jdfidr wotb 4> gc a uctrfpurd wftdr+ F` f stor jfr fk` gavd tbd jdfidr ta f stor pcftd+"xooo/ ^opdttd tbd `ocutd` sacutoak okta tbd 6>> gc a uctrfpurd wftdr vdry scawcy "`rop jy `rop/ wotb fst storrokh+"xov/ Prfksdr tbd `ocutd` sacutoak ta f 4>'gc makomfc tujd fk` rddzd ot ft √ 2> ¾M ar ft cdfst 4 b+ @a kat dxmdd` 0> gc a
sacutoak pdr tujd fs tbd sacutoak gfy dxpfk` `urokh rddzokh+"xv/ Cyapbocozd ar :”= `+"xvo/ Ftdr cyapbocozftoak# trfksdr => gh a cyapbocozd` pra`umt okta f cfrhd :'gc gomramdktrouhd tujd fk` f`` : gc a purd
gdtbfkac+& mfutOak Gdtbfkac os f bfzfr`aus sujstfkmd9 wd rdmaggdk` usokh tbd prapdr sfdty dquopgdkt+ Ok f``otoak#gdtbfkac os f efggfjcd sacvdkt9 tbdrdard# saurmds a ohkotoak sbauc` jd rdgavd` jdard usd+
"xvoo/ Okmujftd tbd sfgpcd ar => gok ft raag tdgpdrfturd "fk okmujftoak togd a akcy 6> gok os rdmaggdk`d` o f pratdokar gdtbfkac'sacujcd `ruh os jdokh dkmfpsucftd`/+
"xvooo/Prfksdr tbd sacujcd partoak ta f kdw :'gc gomramdktrouhd tujd+
"xox/ Mdktrouhd tbd tujds ft 6>#>>> r+p+g+ ar 4 gok ft 0 ¾M ok f gomramdktrouhd+"xx/ @osmfr` tbd gdtbfkac supdrkftfkt okta f prapdr wfstd jattcd fk` `ry tbd pdccdt avdrkohbt ok tbd baa`+ ]tard ft 0 ¾M+"xxo/ Pa rdsuspdk` tbd gomraspbdrds# rst wfsb tbd gomraspbdrds jy f``okh : gc a wftdr ta tbd pdccdt fk` tbdk mdktrouhd
ft 6>#>>> r+p+g+ ar 4 gok ft 0 ¾M+"xxoo/ Zdgavd tbd wftdr fk` rdsuspdk` gomraspbdrds ok tbd `dsord` wftdr ar judr+ O tbd gomraspbdrds bfvd fhhrdhftd`#
tbdy mfk jd `ospdrsd` jy sakomftokh tbd sacutoak ar fjaut 6> s ft =>$ fgpcotu`d "~:> X/+"L/ Gob "pUf/ ◁ tOGOkH : `
"o/ ^rdpfrd f 4$ "wt!vac/ ^UF sacutoak jy f``okh >+:4 h a ^UF ta 0 gc a uctrfpurd wftdr+ Bdft ta 5> ¾M ta `ossacvd tbd ^UF+"oo/ Prfksdr tbd sacutoak okta tbd 6>'gc hrf`uftd` mycok dr fk` f`lust tbd vacugd a tbd sacutoak up ta 4 gc wotb
pfusd paOkt ]tard tbd ^UF sacutoak ft raag tdgpdrfturd ar ka gard tbfk = gaktbs+"ov/ @ocutd soci sacutoak ta 4$ "wt!vac/ wotb uctrfpurd wftdr+"v/ F`` 6 gc a 4$ "wt!vac/ soci fk` 0 gc a 4$ "wt!vac/ ^UF okta f 64'gc makomfc tujd fk` hdktcy gox+ Xbocd scawcy
gavokh tbd prajd up fk` `awk# sakomftd tbd sacutoak ar => s ft :4$ fgpcotu`d+& mfutOak Pbd sakomftokh bark gust jd oggdrsd` ok sacutoak wbocd tbd pawdr os fppcod` ok ar`dr ta prdvdkt `fgfhdta tbd okstrugdkt+ Jd mfrduc kat ta taumb tbd bark ak tbd so`ds a tbd tujd `urokh sakomftoak+
& mfutOak Dxtdk`d` dxpasurd ta uctrfsakomftoak gfy mfusd `fgfhd ta akd‟s bdfrokh+ Tsd a pratdmtovd dfrgus osbohbcy rdmaggdk`d +
"vo/ ^aur tbd sacutoak okta f ^dtro `osb fk` fccaw ot ta mavdr tbd jattag a tbd `osb dvdkcy+"voo/ @ry tbd sacutoak avdrkohbt ok f ugd baa`+
pfusd paOkt Ftdr `ryokh# tbd ocg mfk jd stard` ar f dw wddis+ Mavdr fk` sdfc tbd `osb wotb ^frfocg fk` stardot ft raag tdgpdrfturd+
"vooo/ ^ddc a tbd cg rag tbd `osb fk` pcfmd akd cg ok f 4>'gc makomfc tujd+"ox/ F`` :> gc a `doakozd` wftdr ta tbd tujd9 sbfid ar => gok ft raag tdgpdrfturd ta `ossacvd tbd cg+
1 trauJcdsBaatOkH"x/ Mdktrouhd ft 66#>>> r+p+g+ ar :> gok ft 0 ¾M fk` `osmfr` tbd supdrkftfkt+
"xo/ Zdsuspdk` tbd pdccdt ok 4 gc a uctrfpurd wftdr+ ]akomftd ar 64 s ft 64$ fgpcotu`d# o kdmdssfry+Aptoakfc< usd tbd suspdksoak "maktfokokh jatb kfka' fk` gomraspbdrds/ ar cyapbocozd fk` stard tbd `ry gftdrofc+
"I/ d ej ◁ tOGOkH 6 `"o/ ^rdpfrd f sacutoak a 4$ "wt!vac/ ^DA ok wftdr ok f :>'gc hcfss smoktoccftoak vofc+ Tsd f sgfcc stor jfr ta dksurd
prapdr goxokh+ pfusd paOkt Pbd ^DA wocc tfid togd ta `ossacvd okta sacutoak# sa tbos sbauc` jd `akd fbdf` a togd+ Pbos sacutoakmfk jd stard` ft 0 ¾M ar 6 ydfr+
"oo/ F`` 4 gc a tbd 4$ "wt!vac/ ^DA "?>>#>>> h gac √ 6/ sacutoak okta :> gc a 2$ "wt!vac/ soci sacutoak ta hdkdrftd fkfqudaus sacutoak a 5+0$ "wt!vac/ soci!6$ "wt!vac/ ^DA+
mrOtOmfc stdp F bohb vosmasoty# jdmfusd a pacygdr makmdktrftoak fk` gacdmucfr wdohbt# gust jd gdt ok ar`dr artbd pacygdr gacdmucds ta dktfkhcd fk` arg f ldt+ O dotbdr tbd makmdktrftoak ar gacdmucfr wdohbt a tbd pacygdr ostaa caw# akcy jdf`s wocc arg+ Bdkmd# wd bfvd okmcu`d` ^DA ok tbos pratamac ta pragatd jdr argftoak jy okmrdfsokhtbd avdrfcc pacygdr makmdktrftoak+
"ooo/ ]cawcy stor tbd jcdk`d` sacutoak ar ~6> gok ft 0 ¾M ta ajtfok f bagahdkaus sacutoak+ Fvao` bohb sbdfr `urokh goxokhtbft gfy mfusd prdgfturd mrystfccozftoak a tbd soci+
7/30/2019 Rockwood(2011)_Materials Fabrication From Bombyx Mori Silk Fibroin_Nature Protocols
"ov/ @rfw up 6> gc a tbd soci!^DA sacutoak okta f 6> gc syrokhd+"v/ Fttfmb f 65'hfuhd kdd`cd ta tbd syrokhd+"vo/ Gaukt tbd syrokhd ak tbd syrokhd pugp+"voo/ ^cfmd f maccdmtoak surfmd ft f `ostfkmd a 8”:> mg rag tbd top a tbd kdd`cd+ Gfky surfmds mfk jd usd` ta maccdmt
dcdmtraspuk jdrs fs cakh fs tbdy mfk jd dcdmtromfccy hrauk`+ F sogpcd surfmd tbft maksosts a f podmd a mfr`jafr`
mavdrd` wotb fcugokug aoc os sumodkt+"vooo/ Fttfmb tbd pasotovd vactfhd cdf` ta tbd kdd`cd ak tbd syrokhd fk` tbd hrauk` cdf` ta tbd maccdmtoak surfmd+"ox/ ]dt tbd murrdkt ta scohbtcy fjavd > F+ Purk ak tbd bohb vactfhd fk` syrokhd pugp+
& mfutOak Dcdmtraspokkokh os pdrargd` ft f bohb vactfhd+ Jd mfrduc wbocd tbd bohb vactfhd os ak fk` `a kat taumbmbfrhd` surfmds sumb fs tbd kdd`cd ar pasotovd vactfhd cdf`+
mrOtOmfc stdp Pbd dcdmtrom dc` strdkhtb os rdquord` ta okotoftd tbd ldt fk` ft tbd cawdr cogot jdf` `ddmts frddvo`dkt ak tbd jdrs+ Ft tbd bohbdr cogot# soci jdrs trfksotoak rag f rauk` garpbacahy ta f rojjak'sbfpd` mrass'
sdmtoak+ Ot bfs fcsa jddk katd` tbft wotb fk okmrdfsd ok vactfhd tbd jdr `ofgdtdr okmrdfsds jdmfusd a f``otoakfc gfss trfkspart wotb tbd bohbdr dcdmtromfc armd+ Jy okmrdfsokh tbd `ostfkmd jdtwddk tbd spokkdrdt fk` tbd maccdmtokhsurfmd# tbd jdr `ofgdtdr mfk jd rd`umd`+ Eokfccy# tbd eaw rftd sbauc` jd f`lustd` sa tbft tbd sacutoak os makstfktcyjdokh suppcod` ta tbd top wotbaut fccawokh dxmdss sacutoak ta `rop0:+
jd smfcd` wotb f :><6 rftoa wotb tbd soci gfss ok sacutoak+ Jd mfrduc ta mbaasd f gac` gftdrofc tbft wocc kat oktdrfmt wotbBEO^+ BEO^ os bohbcy marrasovd# sa gdtfc sbauc` jd fvao`d`+ Ok f``otoak# ar cfrhdr smfac`s tbd gdtbfkac trdftgdkt fk``ofcysos togds gfy kdd` ta jd smfcd` up ta dksurd pdkdtrftoak a tbd sacvdkt gacdmucds okta tbd mdktdr a tbd smfac`s+
"v/ "Aptoakfc/ O yau frd f``okh f rdokarmokh fhdkt sumb fs by`raxyfpftotd# wdohb ot fk` f`` ot ta tbd sfct bdrd+ Gox wdcc#jatb ok tbd wdohb `osb fk` ok tbd gac`+
"vo/ Pfp tbd maktfokdr hdktcy ak tbd jdkmb tap surfmd ta cdvdc tbd sfct ok tbd maktfokdr+"voo/ Ok f ugd baa`# f`` 6 gc a soci sacutoak ta dfmb maktfokdr a sfct vdry quomicy usokh f 4'gc syrokhd+ Oggd`oftdcy mfp
Fccaw tbd gdtbfkac ta sddp tbrauhb tbd smfac` ar 6 `+"xo/ Zdgavd dxtrf gdtbfkac fk` `ospasd a ot ok f prapdr wfstd maktfokdr+ Prfksdr tbd maktfokdrs ta f :'cotdr jdfidr a
uctrfpurd wftdr "~5 maktfokdrs pdr : cotdrs a wftdr/+
1 trauJcdsBaatOkH"xoo/ ^cfmd tbd jdfidr ak f stor pcftd fk` stor+"xooo/ Mbfkhd tbd wftdr :”= togds f `fy ar :”= `+"xov/ Zdgavd tbd smfac`s rag tbd gac`s fk` rdpcfmd tbdg ok rdsb wftdr ar fk f``otoakfc `fy+ ]mfac`s wotbaut
rdokarmdgdkt wocc jdhok ta eaft wbdk tbd sfct os rdgavd`+"xv/ ]tard smfac`s ok uctrfpurd wftdr ok 4>'gc tujds ft 0 ¾M uktoc kdd`d` ar usd "mfk jd stard` ar :”5 gaktbs/ ar `ry
tbd smfac`s fk` stard tbdg `ry ft raag tdgpdrfturd+"xvo/ Xbdk kdd`d` ar usd# mut tbdg ok wftdr ft `dsord` `ogdksoaks fk` futamcfvd ta stdrocozd+ Xd suhhdst scomokh a tbd
tap fk` jattag cfydrs fk` `osmfr`okh tbdg# fs tbd surfmd bfs f siok tbft os cdss paraus+
1 trauJcdsBaatOkH
1 trauJcdsBaatOkHPraujcdsbaatokh f`vomd mfk jd auk` ok tj :+
"maktokud`/
tfJcd : | Praujcdsbaatokh tfjcd+
s pjg so
64 "soci pramdssokh/ ]oci `ads kat ossacvd ftdr 0 b
ok cotboug jrago`d "CoJr/
@o` yau fmmaukt ar tbd vacugd a CoJr wbdk prdpfrokh tbd ?+= G sacutoak1
O kat# f`` tbd fppraproftd fgaukt a CoJr+
@o` yau jaoc tbd mamaaks ok sa`oug mfrjakftd ar => gok1 O yau jaocd` ar f
sbartdr togd# yau gfy kdd` ta fccaw tbd soci ta ossacvd ok CoJr ar cakhdr+ Gfidkatd a tbd jaocokh togd fs jftmbds gfy vfry o tbd jaocokh togds frd odrdkt+
@o` yau paur tbd CoJr avdr tbd soci ojdrs1 O kat# yau wocc kdd` ta gox tbd
sacutoak ta dksurd maktfmt jdtwddk tbd soci fk` tbd CoJr sacutoak+ Jd surd ta
mavdr tbd jdfidr ta rd`umd dvfparftoak+
Mbdmi tbft tbd avdk os sdt ta 5> ¾M
CoJr fk` soci ojraok sacutoak os
kat fgjdr macard`
Mbfkhd ar mcdfk hcfsswfrd
:4F"oo/ "tujds/ ]oci wocc kat dvdkcy maft tbd
word fk` sdvdrfc jdf`s frd
prdsdkt fcakh tbd gfk`rdc
]oci os kat makmdktrftd` dkauhb# stard ft 0 ¾M ar f dw fys# mbdmi ot
pdroa`omfccy fs tbd soci wocc hdc o ot os stard` ar taa cakh
7/30/2019 Rockwood(2011)_Materials Fabrication From Bombyx Mori Silk Fibroin_Nature Protocols