ISSN 2349-3690 RRJoVST May-August 2014 Veterinary Science Research & Reviews: Journal of and Technology Antioxidant Enzyme Status in Broiler Chickens Biochemical Assessment of Allantoic Fluid in Pregnant Sheep Biochemical Changes in Pregnant and Lactating Kilakarsal Ewes Role of Pheromones & Biostimulation in Animal Reproduction Treatment of Mange in Rabbits: A Case Report Torsion of Uterus as a Cause of Dystocia Mortality Due to Pasteurellosis in Calves STM JOURNALS Scientific Technical Medical STM JOURNALS Scientific Technical Medical STM JOURNALS Scientific Technical Medical
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ISSN 2349-3690RRJoVST
May-August 2014
Veterinary ScienceResearch & Reviews: Journal of
and Technology
�Antioxidant Enzyme Status in Broiler Chickens
�Biochemical Assessment of Allantoic Fluid in Pregnant Sheep
�Biochemical Changes in Pregnant and Lactating Kilakarsal Ewes
�Role of Pheromones & Biostimulation in Animal Reproduction
�Treatment of Mange in Rabbits: A Case Report
�Torsion of Uterus as a Cause of Dystocia
� Mortality Due to Pasteurellosis in Calves
STM JOURNALSScientific Technical Medical
STM JOURNALSScientific Technical Medical
STM JOURNALSScientific Technical Medical
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Research & Reviews: Journal of Veterinary Science and Technology
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(ISSN: 2349-3690)
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Dr. Girish Kumar MishraAssistant Professor College of Veterinary
Science and Animal Husbandry,Chhattishgarh Kamdhenu Vishwavidyalaya India.
Dr. Gopala ReddyProfessor & University Head, Dept of
Veterinary Pharmacology & Toxicology, College of Veterinary science,
Rajendranagar, Hyderabad India.
Dr. Kaushalendra KumarAssistant Professor,
Department of Animal NutritionBihar Agricultural University,
India.
Dr. Aditi Dixit Assistant Professor,
College of Veterinary Sci.Durg (C.G.)
India.
Dr. Gyandev SinghAssistant professor,
clinical complex, B.V.C PatnaIndia.
I take the privilege to present the hard copy compilation for the [Volume 3 Issue (2)] of
Research&Reviews: Journal of Veterinary Science and Technology (RRJoVST).The intension of
RRJoVST is to create an atmosphere that stimulates creativeness, research and growth in the area of
Veterinary Science and Technology.
The development and growth of the mankind is the consequence of brilliant Research done by
eminent Scientists and Engineers in every field RRJoVST provides an outlet for Research findings
and reviews in areas of Veterinary Science and Technology found to be relevant for National and
International recent developments & research initiative.
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the advancement and dissemination of Research results that support high level learning, teaching and
research in the domain of Veterinary Science and Technology
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their continued support and invaluable contributions and suggestions in the form of authoring write-
ups/ reviewing and providing constructive comments for the advancement of the journals. With
regards to their due continuous support and co-operation, we have been able to publish quality
Research/Reviews' findings for our customers base.
I hope you will enjoy reading this issue and we welcome your feedback on any aspect of the Journal.
Dr. Archana Mehrotra
Director
STM Journals
Director's Desk
STM JOURNALS
1. Antioxidant Enzyme Status in Broiler Chickens: Role of Dietary Supplementation of Ocimum sanctum, Ocimum basilicum with Organic Selenium V. Leela, L. S. S Varaprasad Reddy, G. Suganya, K. Loganathasamy, C. Devaraj 1
2. Biochemical Assessment of Allantoic Fluid during Different Stages of Pregnancy in Madras Red Sheep C. Devaraj, V. Leela 5
3. Biochemical Profile of Kilakarsal Ewes during Pregnancy, Post-partum, Lactation and Dry PeriodM. Sundara Vinayaki, V. S. Gomathy, C. Devaraj 10
4. Role of Pheromones and Biostimulation in Animal Reproduction-An OverviewR. P. Tiwari, Abrar Ahmed, G. K. Mishra 15
5. Treatment of Mange in Rabbits: A Case ReportAditi A. Dixit, M. L. V. Rao, G. Das, Kabita Roy, Virendra K. Thawait, Vijay Bhaskar U. 21
6. Torsion of Uterus as a Cause of Dystocia in a Nellore Brown EweK. Jyothi, M. Mutha Rao, Y. V. Pridhvidhar Reddy, A. Ganesan 24
7. Mortality Due to Pasteurellosis in CalvesSanjiv Kumar, S. Samantaray, D. Kumar, S. Kumari, R. Kapur 27
ContentsResearch & Reviews: Journal of Veterinary Science and Technology
Research & Reviews: Journal of Veterinary Science and Technology ISSN: 2319-3441 (online), ISSN: 2349-3690 (print)
Volume 3, Issue 2
www.stmjournals.com
Antioxidant Enzyme Status in Broiler Chickens: Role
of Dietary Supplementation of Ocimum sanctum,
Ocimum basilicum with Organic Selenium
V. Leela, L. S. S Varaprasad Reddy, G. Suganya, K. Loganathasamy, C. Devaraj* Department of Veterinary Physiology, Madras Veterinary College,
Chennai, Tamilnadu, India
Abstract An experiment was conducted in broiler chicken to evaluate the effect of dietary
supplementation of Ocimum sanctum, Ocimum basilicum and selenium on antioxidative
enzyme levels. Total of forty two broiler chickens of day old divided into 6 groups of 7 each were used for this study. O. sanctum leaf powder (0.5%), O. basilicum leaf powder
(0.5%) and organic selenium (0.3 ppm) and their combinations were added to the basal diet. Superoxide dismutase (SOD), glutathione peroxidase (GSH-px) and catalase levels
in plasma were measured at the end of 3rd
and 6th
week of age. Dietary supplementation
of selenium itself significantly (p<0.01) increased GSH-px activity and supplementation of both Ocimum species significantly increased SOD and catalase levels. However, O.
basilicum and O. sanctum in combination with selenium effectively enhanced the levels of
SOD, GSH-px and catalase. Between O. basilicum and O.sanctum with combination of selenium, no significant variations in antioxidant levels were seen. Both the plant sources
showed similar potency in scavenging free radicals. It is concluded that dietary supplementation of O.sanctum or O. basilicum at 0.5% level and its combination with
selenium (0.3 ppm) can combat oxidative stress in broilers, by enhancing antioxidative
Research & Reviews: Journal of Veterinary Science and Technology ISSN: 2319-3441 (online), ISSN: 2349-3690 (print)
Volume 3, Issue 2
www.stmjournals.com
Biochemical Assessment of Allantoic Fluid during
Different Stages of Pregnancy in Madras Red Sheep
C. Devaraj, V. Leela* Department of Veterinary Physiology
Madras Veterinary College, Chennai, Tamilnadu, India
Abstract To determine the biochemical constituents of sheep allantoic fluid during the different stages of pregnancy, sixty sheep uteri at various stages of pregnancy were collected from
the local slaughterhouse. The allantoic fluid samples were collected from each gravid
uterus. Foetal age was calculated according to crown anus length of the embryo/foetus by using the age estimation formula. At four stages of pregnancy viz., – I (30–60 days),
stage - II (61–90 days), stage - III (91–120 days) and stage - IV (121 to term), the
biochemical variables measured, respectively, were: glucose concentration were 8.13, 6.80, 4.30 and 3.21 mg/dl; total protein, 0.58, 0.62,0.79 and 1.03 g/dl; urea, 29.23,
36.03,51.68 and 67.06 mg/dl; creatinine, 5.98, 9.70,17.66 and 24.92 mg/dl, cholesterol, 3.08,3.32, 3.54 and 2.88 mg/dl; sodium, 94.20, 100.9, 89.00 and 97.86 mmol/L;
potassium, 4.40, 5.72, 4.26 and 5.50 mmol/L; calcium, 8.73, 8.67, 5.9 and 4.20 mg/dl and
magnesium, 0.32, 0.81, 0.22 and 0.01 mg/dl. Significant correlations (P<0.05) were found between gestational age and levels of glucose, total protein, urea, creatinine and
calcium. These findings might be helpful to understanding the foetal metabolism and
diagnose pathophysiological conditions during pregnancy.
Research & Reviews: Journal of Veterinary Science and Technology ISSN: 2319-3441 (online), ISSN: 2349-3690 (print)
Volume 3, Issue 2
www.stmjournals.com
Biochemical Profile of Kilakarsal Ewes during Pregnancy,
Post-partum, Lactation and Dry Period
M. Sundara Vinayaki*, V. S. Gomathy, C. Devaraj Madras Veterinary College, Chennai-600007, India
Abstract The present study was conducted to investigate the changes in biochemical parameters
during different physiological states of reproduction. The Kilakarsal sheep reared at the
Instructional Livestock Farm Complex, Tirunelveli, Tamilnadu, housed under standard management conditions in sheds with an open paddock was chosen for this study. Twenty-
four ewes were categorized into four groups of six each at different physiological phases
of late gestation, post-partum, lactation and dry period. The average live body weight of ewes in this study was 35 ± 1.62 kg. The study showed no significant difference in certain
biochemical parameters like magnesium (mg/dL), urea (mg/dL), creatinine (mg/dL) and non-esterified fatty acids (NEFA) during late gestation, postpartum, lactation and dry
period. However, significant (p < 0.05) differences were noted in certain biochemical
parameters like total protein (g/dL), calcium (mg/dL) and phosphorus (mg/dL) amongst the four groups of different stages.
Research & Reviews: Journal of Veterinary Science and Technology ISSN: 2319-3441 (online), ISSN: 2349-3690 (print)
Volume 3, Issue 2
www.stmjournals.com
Role of Pheromones and Biostimulation in Animal
Reproduction-An Overview
R. P. Tiwari, Abrar Ahmed, G. K. Mishra* Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science
and Animal Husbandry, Anjora, Durg, (CG) India
Abstract In recent years, considerable progress has been achieved in the comprehension of profound effects of pheromones on reproductive physiology and behaviour. Pheromones
have been classified as molecules released by the individuals & responsible for elicitation
of specific behavioral expression in members of the same species. These signaling molecules, often chemically unrelated, are contained in body fluids of urine, sweat,
specialized exocrine glands and mucous secretion of genital. In mammals, signaling and
priming pheromones are thought to act either singly or in combination through olfaction, auditory, visual or tactile stimuli. Biostimulation or male effect is the stimulus provoked
by the presence of males, which induces estrus and ovulation through genital stimulation, pheromones or other external cues. The biostimulation technique offers a potentially
useful and practical way to improve reproductive efficiency in livestock species. The aim
of this manuscript is to examine the role of pheromone and biostimulation in animal reproduction that would provide information of interest for animal husbandry, where
economic circumstances do not allow the use of expensive and invasive techniques for
Research & Reviews: Journal of Veterinary Science and Technology ISSN: 2319-3441 (online), ISSN: 2349-3690 (print)
Volume 3, Issue 2
www.stmjournals.com
Treatment of Mange in Rabbits: A Case Report
Aditi A. Dixit*, M. L. V. Rao, G. Das, Kabita Roy, Virendra K. Thawait, Vijay Bhaskar U. College of Veterinary Science & A.H., Durg (C.G.), India
Abstract A pair of rabbit was presented with the complaint of severe pruritis, alopecia,
inappetance and dullness. Skin scrapings revealed psoroptic mange infestation.
Treatment with ivermectin @ 200 µg/kg body weight along with antibiotics and liver extract preparation revealed clinical improvement in 14 days and complete recovery in
Abstract A Nellore brown ewe in its first parity was presented to the clinic with a complaint of dystocia. Pervaginal examination revealed right side post cervical uterine torsion.
Detortion of uterus was achieved by rolling the dam by modified schaffer’s method.
Torsion relieved but cervix was not dilated fully necessitating caesarean section but the owner preferred to dispose of the animal.
Keywords: Nellore brown ewe, Dystocia, Uterine torsion, Modified Schaffer’s method
Research & Reviews: Journal of Veterinary Science and Technology ISSN: 2319-3441 (online), ISSN: 2349-3690 (print)
Volume 3, Issue 2
www.stmjournals.com
Mortality Due to Pasteurellosis in Calves
Sanjiv Kumar1*, S. Samantaray
2, D. Kumar
1, S. Kumari
3, R. Kapur
4
1Department of Pathology, Bihar Veterinary College, Patna, Bihar, India
2Deptt. of Parasitology, BVC, Patna, Bihar, India
3Deptt. of Microbiology, BVC, Patna, Bihar, India
4Project Veterinary Officer
Abstract Mortality was recorded in fifteen Jersey calves in the cattle farm of Bihar Veterinary
College, Patna. All these calves before death were showing symptoms of high fever, severe dyspnoea, mouth breathing, profuse salivation and oedema in throat region. Dead
animals subjected to necropsy showed gross and histopathological lesions of
Haemmorhagic Septicemia. Stained heart blood smear and culture showed the presence of bipolar coccobacilli Pasteurella organisms. All the other ten ailing animals responded