REPUiLT KI! - DTIC · 2018-11-08 · a&ent was differ•,nt froan all viruces existing in the laboratory arid was sent to the WEO World Refere:OSe Center at Yale University for final

REPUiLT KI!.ILER2

ARBOVIRIS SrJ*DTES 1" S!AC lkFLLO FRAZIL

LA JUI RIJCRT

by

jAUTJARY 16

Defen;se Msearch Office for L~:.AmericaM~e 69Jwarefo, Brazil

DA-1FC2 9-6'7- G--CD10

U.S. Depart-unrt of the Army ProCecý. Number 3AC214O1BiDrP

Irx.sI1tuto AL"olfn' Lu'tz

II

CLIM )TIC

THIS DOCUMENT IS BEST

QUALITY AVAILABLE. THE COPY

FURNISHED TO DTIC CONTAINED

A SIGNIFICANT NUMBER OF

PAGES WHICH DO NOT

REPRODUCE LEGIBLY.

Th6 studiesa on tho epidenido.Iogy of -.he Arbovirumes in Sio F&%uao,Bres1l, were eci~tinued as outlinod 1ithe la~.s- ye.r rt~port.

The routine tc isclate viruqfes Ei nC LL' C01!P(-t the saMP:es hasa! ready been desc-r ýbed in prevIlous rEpl.rts.

The agente I.Eclateci &nc e: c.h* z ye!:''re 8Lcwn ir; TvielI. t car. be seer. thnt 14 of ther. wore lsoivnied fr-inr lirds cpaptured In

.the stu~y ereas. The araswerc netted : t-rg te -E;:tcd froir&vteri1bker166 to A4r-1P67 nnd 1:y OF zesta the-' are al.I !1er:-"Cpi1* see~r-Ingto be ýIflerent cif~nsc the sa.me viras, Neutrai'zation taatz inTO arý L*r. pr:-rEsv:.

Thne wa~t-p ~os: s Ay- ",-45, tht: `ýrst '1c.L'-,e 1*r Ctheit s`,win to be anfran't. s-it -: .cre trtor 3 1(;,- to

DC.A w~.ail ZT ý,7* fcr sý ický-rg ~'e_:ofitdirtrucceibeta1i(ILC) rin, 4,e' :c, -. -cc intra.,erituoria4.y (IF1). h viruskilfle -i ".2i -1-e IC inr -dyrcc tos inocuItated IP

survi ve-l an -cce~f r CF ti'tex- thre' r seron. Thle spent W~ifi~~r2 trcfr liu~Fil~ter withr remtenrac, p..rt -Size of 510 r!&.,

Ant reý -.:: 0. t s:icrcse-ýcutoz,e ex~lrcction --f' the babjyri1ce sh'--oec a b' i7~.-]lztin (HA) witn, titer of' 160 fcr gceee69)25, anild p~ccd CF ant't-n Antigens ci taty mice seýra -,nd liver 21*dnot Ehow a 1-A aci ger.i- r'S prodiýce a Fgood CFE in BhK-211 an!i

Ti-e v'r~ s Wa-15 LeStvPo lro ý& nd Or tOL~ts aga~nst the fo2-ici(ng

vizso~: ~E WI, J. ' u.-o -L'c~ Yarirc, Yello,-w Ffiver (Asib,)SLI, Buosuqtuara, lihetta, CarpporL,:, Orlj c ,, Guarov, Cache Va~lley, Ma-pwari, Ca2r~.BFS,, annory, Itapocrziný;, Anl-Loza, BarLacet-Is, J~uýI!,.

Tacmr,~w ~ Gtiar~aCatu~, Capiu 3:<1o, Eu.~h-hu~sh, Marnzan ala,I~.1'ioArc c ~ A, irb Tur.LcQc', Cov;J, Trituia, AcrY?~r Cotia.

No coVS' r t co We're showýn. If. oeerrned to L~s tha' wr: have an agentDUA seno' I E dlfferurt cf aLA cx.: sing ;--r the Llrtr-aivi problably

an rbo re .y s be*hav.'sý,:r, The agent was saunt tu- Yale Arli iPasearce !',L t 'Or furzhur ztudies.

Ano 'I~. v"i rts, Ar 5ý'SL q, 1~ is,ý-at~ed f:,a r(cd or) 1X Ac-3kC56WZ& ca-tum<6- inIa:erig or 10/11-/66. It k~i'l&, lba- ,Ilco IC

w' th ar: AST o' 3,1 anc; 1u c C w't'- an AST of 7.0). 1 L ~i-; killautC~~~~~~~~ e~ icyit Q Fw * yd nm era wo s ob,~u~ Ant *;a

preparedi by 'v- -i-"tc e.t roction ol' laWt~ r..>e i: .livczr. aridsere r1-'d no' Yu:i nn HA CTFO terts :' rt.wctt 'ci wrao CAl-'trieci ..;'.nerbe*-rs ';f C< " Cn'x, L;rf the strc'n,-fs wlt. 4I.'e.i5Lo virus.We-:trl.' :z& 4Acn Ltsts e - rrccr(;-ul ta see If -he viriis is a i~so1~tionof' Malft,' virus ;n cur ýicc ,f r nt~v viru-s .r. "tic Cal ifXrr.ni C(Ui.p2ix.

kr,-ther vi:~awezr ;dcirt til'd by now. Ar 291824 aoni-Ar 2546 arethe a-ýue ls~laricn ef i .- rum belorffrng t(- the Punysr~wr:. Grouip. This

a&ent was differ•,nt froan all viruces existing in the laboratory arid wassent to the WEO World Refere:OSe Center at Yale University for finalide tiflaation. A study of the local population is being made;, as somefor the human sera here are able to neutralize the agent.

An 3088, Ar 2573, Ar 2494 are new strains of Cotia virus (Am.J.Trop.Med. & Hyg., J&: 156, 1965). These isolF.tions were the firstobta-ned in Gasa Grande of a virus isolated in another field statior.Its presence in the humau population is under study.

Ar 5507 is an agent isolated from 100 Apcr collected on.1/15/67 in Casa Grande and it is a now Isolation of Boraceia Virus, Ar395.

OLI-er v-'ertses are being studied. Ar 4253, isolated from Cotiao01 1/2/66 of a pool of pro , Ar 6171 from a pool of V_•

m~.a cfus~. collected in ', rE Grande on 12/2/66 and Ar 6629 from apo2l of C - collected ir. Casa Grande on 2/3/67. They seemed to bedifferent frc:: the agerts isolated by this !.nboratory and theirident~ficfticn are in iprogress.

Ar 6629 was a very interesting isolation because was obtEinedfrom •osqjuitoes collected inside of the houses of the village.

In spite of the work done by us with the help of the localsch*!c teacher, we continue to face a strong resistence from the localpopulction tc accuse any illness.

We obt1T-•ed sawples from 2 febrile cases from children attendingtne school. The viral isolations were negative and the serclogy, usingall the nrtigens that we ha•o in the Laboratory also did not shown anyrise in titer for Arlovirms.

Frcr a §mall city near the border of the State of Parana werece'ved blood frcm 7 children withl, fever, headache and signs ofe'•vclvixert of nervous system. All of them were recently vaccinateda-'*it Yellow Fevwr. The tentative of viral isolations were nec'-tiveanr' no xse "In titer for arbovirus was o'served in the serology. Theserolc'_ical survey made with sera collected in 1966 in Casa Grande wasalrost cor..pletcd. The total resalls are shown in Table Iio

It ,-a- be seen that the results are almost tho same .:btq,'-oJ inprvrlous bietd'dEn. Tacaduma and Borace'a virnses are the more commonin the arca with a prevale:nce of 10% for Borceia viruts with 7con- rt.]crns to positive and 165 for Tacaitra virus, with 14 convertions.0Other ate, ts isolated loculy were also present in the populaticn.

Ar 4:75, qn agert relrted to Boraceia virus was positive foroa-y 9 people shcori Cg a snaller prevalence than Borace!a virus.

Ar 2984, a mer her cf the Bunyerwera Group vas also positive in7 sera. with prevalence smaller than Borace!h and Tacaim-ia viruses.

The antibodies for the B Group were present agtimr, in the levelsobtcincie. previcusly. Bussuquara was the more common vIALs fourn d, butthe titers were Jcw and the pattern seemed to be a cross reaction, ai.1bserved previously. We detected 12 convertions.

Fý

r hcr;e

BII

Virus Isolated in 1967 by Source and by Field Station

An 5417 - Myiobiun atricauduo - Casa Graxde - 28/ 7/66An 5560 - *onpophaga ineata - Casa Grande - 19/ 8/66An 5632 - Xanthamyao virescens - Itapotiznnga - 26/ 8/66An 5742 - Schiffornis virescens - Casa Grande - 1/ 9/66An 6109 - Dandrocolaptes platyrostris - Itapetininga - 24/11/66An 6242 - Dysithamnus mentalis - Casa Grande - 1/12/66.An 6157 - Haplospiza uxicolor - Casa Grande - 1/12/66An 6212 - Sporophila caerulescens - Itapetininga - 3/ ý6An 6236 - Sittaesomu griseicapillus - Casa Grande - 16/11/66An 6241 - Basileuterus auricapillus - Casa Grande - 16/12/66An 6291 - Platyrirchus mystaceus - Itapetinirga - 23/2/66An 6789 - Tardus albicollis - Casa Grande -3/ 3/67An 6829 - Enberizoides herbicola - Casa Grande - 10/ 3/67An 7126 - Platyrinchus mystaceus - Itapetining - 6/ 4/67

An 4229 - Philander opossum - Casa Grande - 14/ 1/66

Ar 4253 - Psorophora ferox - Cotia - 23/ 1/66Ax 6171 - Wyecmxnia cor-Osaa - Casa Grande - 2/_Wý6Ar 6629 - Culex ap - Casa Grande - 3/2/67Ar 6813 - A.(K.) cruzil - Casa Grande - 9/ 3/67

* -- Pools

TAEbLE I]

Results obtained in HI and N'T tss v-li. Hwriw. Sera f~rom 1966

in Casa Gmicle

VIRUS R.ESUJLTS VIRUS RE~SULTS

Grcup A. C/242 Btinyanwera Gimup 7/242

EZEG"n ro '.i

WEE Cache Valley

Mayrro Ar 293A4 7

Mucaxmho_____________________Callfcnx*a 0/242

G~roup B 26/242I

Busuquara 24 Group Pl~ebotcrnus 1/242

SLE 22 Itap.oraiiga -

IF 5 Icoaracy

I.1heus 1.5___________________________ Tacaiuma-

G',roup C 8/242 (SP Ar 2317) 47/242

Marituba Boravels,

Caraparu 3?A 952/

Oriboca 6 SPAr 395 5/,42-

*Number ofr positives / Nuinier of tested

6

TABI4 II

Poole off Mosquitoes Inoculated in 1967

SPECIES Grad.s Itapet. Cotia Total

A-t-n.1a1es cruzii 108 1 1 Ji10strodLe! 1 18 19lutzi 4 3 7

Aedes fltzviatilis 1 1l euoocelaermus 3 1 4

Sserratus 14 10 4 28" oriniffer 3 1 4

Cu~lex (1Melanocon-ion) sp 2 3 4 9" (Microcullex) sp 2 2

Yhns.~nia albif erm 2 4 13 19" venezuielensis 1 1" titilans 1 7 8

Psorophora albipes 2 2discruciamg 2 1 3forox 15 30 9 54

Uranotaerula d- taeenonota 1 1

IaiDatus flavisetosus 1 1 2Phoniomdyia pi:licauda 78 2 4 84Sabethes albiprivu~s 2 1 3

" inter'edius 7 7Trichoprasopon digitatum 1 1

pallidiventer 35 2 3 40)reversum 22 1 23

Wyeomnvia confusa 24 4 1 29"R leucostigna 1 1

Tribchposopron ffluviatilis 11 11

Others Dipteroua

Phlebotarius sp 2 2Siznulium au~ristriatum

TOTAL 333 68 7,4 475

Z~& ~7

Birds Netted From Auigust 1966 to Decem~ber 1967

SPECIJB Itapet. Crasa Total

Cryptu.rellum obsoletuu 1 1

Lccipiter erythronamium 1 1

Micresatr ruficollig 1 1

laterallus sp 1 1

Colunbigallina talpacoti 69 69Imptotila rufacl12a. 7 7Oreopeleia, montana 4 6 10

Dromococcyr: pavoninus 4, 4Crotophap-a ani 5 5Gu'.ra gal ra 2 2

Triclaria male chitacea 1 1

Otus choliba, 2 2

Hydropsali~s brasilianna 1 1Eleut' -ptus amamnall 1 1

Mfto'awma rtibricauda. 1 1

Cho eyeamericana, 7 7

B&coo vp 1 1

Crywaptilus melnnochlorou 1 1Pic~mll temianckii 6 6

Deniracolqteu platyrootris 9 9 18squamatux 1 1

Lmpidwoo ptqv fuacua 27 35CampyozI.T!AmvbvA trachilirostrio 7 2 9Sittasqpum griucicapills 15 2.3 38

Birds Netted f'rom~ August 1966 to December 1967 -Cont,

SPECIES IItapet.j Caa Tot&1l

2 7&axt rul'icapiLua 03 1Anbaenp ucophbulx 42 2 2

cydcyluosutariinatua 15 5 80Xenceps minuts 2m~roi 2

Pb-y~o i,.trlplla z 1 2Slnrua scrr 4 4

Cleoolaptes riemura hr- 3 931Helobltu coariidaes 1 7Batara cineres 3 2

Mackenieraleasl 1 1

ruficapillusrs~ 4 4Loylilha~ns nmentual 13 16 29Fynothemjla ulrsDatryipla maurea 4 4Py-kr~zigena leacptr 21 1 19Clha:-nopheza caerpruisom s 12 13

M~r~ceusrufricpisus 5 4

Conopopx-4s meixetall 55 20 75

Aymttilemlat 1uai 2 3"D mphoeiciialra~ 6 4

Pacyramphuea plyucopteruB 2 1 39

Fro..cdruis nqudicollls 1 15FCnno-opaed

C1Tirog~ipl adata17 10 1Iiais eua itari 14 14

Atct 11farud iru~e 18 28 46Nepelxna rd unizrn 2 6 2

B.' rn~ No! F r-c; ALugust. 1966 to Docur-bur' 1967/- Cun~t.

sPEC:ES It spet. Crava Tot c.1

Satrap& ictcT-cphryN 44

Tyrain~us~reaibL~u 5 5

Si rystes sb'latric .1 1Pi*:,,rgua sL1"uratu~s 5 5Mylarc!.fus tyranruluus 2 2

~fc xeu-le-r. 3 2 4 36My-.c~b-'un atrlcaudus 12 .22X,-ICqtiý'U~s f~~~tE24 .14Plht. r' r-,.L..S ,nr2,tCFLLS 42 36 78

Tc~i.y ~ BeeiL'COr's 2 3 5

r s tciven.tra.ýS 1 9Se ucc3rL'stata 5 5

a 2;1'1L~ns~'30 3 33~J'j"P49 5 54*~rst~a2 2

Qui' tc- 6ma 2 2Tiiptopogon aznaurccephaluo 9 9P ~-n.~nf1e~rs 17 62 7

Pri'Eo r ,,.aTr:ý Itsca11

Alupocr.&llor. futcata11

I Tj-~1y'i ~6 6

.. :r ~~c4 4

TAlE,, 30 3-1. 61" ~ I: ~39 3 42

"I OLa.& 2 2 4" ruf renr.P ilg 5 9 2-4 83

F- 't.ycICKIa1 f1rivipemi 3 2 3 2

53 8

~LJX

Birds Hatted From August 1966 to Deosiibor 1967 - Cont.

spzCIES Itapet. Caa Total1

Vireo obhivi 18 2 20H~y2oplillus poicilotis 8 8 16

Dacnisa cayasna 2 2Coereba. flaveola. 3 3

Gsothajpia 4equirictia1ll 3 3Besiftjoua loucablopbarusa a 5 13

bypoleuc 314 14auxricapi.11u8 1 10 11

Tanagra, pectoralis 6 6Pipraeidea, melanorwta 1 6 7Tuzngsaa seledoru 4 4

N cyanocephala /+ 4"f desmaresti 9 9

" cayana 14 *14Thraupio cyanoptera 13 13

" eayace. 32 1 33Orthogonys chloricterus 3 3

Tachyphonuza caranatua 65 16 81Trichothrewipis melanope 12 48 60Neothranpile fasciata M I.Schistochlauyu melanopio 20 20

lMolotbnwi boitriensis 2 26Chr~imopoar chopi 2 2

Saltator similis 10 10 20Tiaris fuliginosa, 1 1 2Sporopbila caerulescens 38 38

R plumbea I 1VoJlatinia jacarina 9 9Spiriuo magel.lanicus 5 5Sicalis flaveola 6 6klaplospiza unicolor 2 19 21Arromoru taciturn w 5 1 6Myoopiza humeralis 12 12Zonotrichia capensio 149 149fnberizoides herbicoln 7 7Donacospiza albifrons 2 2

TOTAL 1 362 78

T rap e d

SEISCASA ITAFET. TOTAL

16~sncrvrg cu4a 35 1, 36" rattua 22 23 45

Musa ruscausi~ I I

Ory-zan"r nigripos 21 1 1"R laticeps 2 2

' s ubfiavtq 1. 1" cap.1to 12 12

Delcmyir dorsalia 3 3Nectoi.;ý squFwrlpes 4 19 23Akodoni arvio.ulcides 35, 1 54Thaptornys nigrita 4 1 5O.-qnyctenas qua3estc~r i3 13

Coerdan insidiosuu .- 1

Caia aprea 3

Prcfclirwys iherlmgi 19 19CLIyor~yrs lat jeeps 10 10

Not idr~ 15120t7

Thioelrhis mars-upulais 14 3Moroc~e-'.his tricolort 2PhilanCder a-)ossurn 66

Carrolia persulcilrata 2

Dsr-r'us rotuz,.us 1.

71n-Igsni jptSecurity Classification

DOCUMENT CONTROL DATA - R&D(Security classification of title, body of abstract and indexing arrnttation mus~t be en~tered .fAen the ov'erall report iscasiid

¶ ORIGiNATIN 0 ACTIVIITY (Corporate author) 2. REPORT SECURITY C LASSIFICATION

Iflstitu'.'o fhdolfo 7_uz-, _Lai~orat~rio de Arjbovirus, UJnclassifiedSao >&,ulo, 3rasi-l 2b GROUP

3 REPORT TITLE

4 DESCRIPTIVE NO~rES (Type of report an~d inclusive date.)

tLnnual Technical .epor *'- period Nlovember 1966 - January 19675 AUTHORM(S Last name. firet naete initial)

7.opes, Oscar de Sou~a

6 REPORT DATE 78 TOTAL NO. OF PAGES 7b. NO. OP REPS

January 1967 12@a CONTRACT OR GRANT NO. 9A. ORIGINATOR'S REPORT NUMBER(S)

Dl.-HC19-67-1-OO10b. PROJECT NO.

211014SC1371D 00 02C ~S9b. OTHER REPORT NOES) (Any othetriumrber that may be a.. neid

thia report)

d

10 AVA IL ABILITY/LIMITATION NOTICES

Unl~itaited

11 SUPPLEMENTARY NC.TES 12 SPONSORING MILITARY ACTIVITY

U.S. AzIr.7 'le..ant, '.e~ene .esearch C..ficeNone Latin Aw~erica

'J. ;. lobansy, L.ilewi York 0967613. ABSTRACT ý-eoorts on con~inuation of. work discussed previously. C1ollections; of

birds, animals, ary ic -h:-.!an sera have been studied, isolatlinr and screening ' virusesdetected. A :Few, neti, or net?' isolations in the study area havi been sent to ýheYale Arbioviruz Labomiatory 'or xfinal. identification or characterization.isolations were also made ;from pooled mosquitos collected in the forest, outsicleofl houses, and inside of houses. Dmtinued di:'licult; ,experienced in obtainin-,speciinens -from ill. inhazi.tants reticent to re',ort illness. :1vidence continucr'ýo ,.ndiczLce transm~ission lrora :-orest4 reservoirs . n,,o houses b,,' wild mosquitor3.-concept, eviCence, reia~ini~ng prolbleris discussed. -.ive tables of data included.

DD FOOMG1473 U~nc lassifiedSecurity Classification

St-tuiiItI,% Cl--ifi('it ion

14 KEY WO•RS LINK A LINK 8 LINK C___RO•E AT ROLE ArT~ o FOL ATr

Virus i

Hemorrhagic sever3pidetioiogyiachupo Virus IJunin li:usArboviru3

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