1 PROFORMA – I PROFORMA FOR SUBMISSION OF PROJECT PROPOSALS ON RESEARCH AND DEVELOPMENT, PROGRAMME SUPPORT (To be filled by the applicant) PART I: GENERAL INFORMATION 1. Name of the Institute/University/Organisation submitting the Project Proposal: PSG Institute of Advanced Studies, Nanotechnology Research Facility, Post Box No. 1609, Avinashi Road, Peelamedu, Coimbatore-641 004 2. State: Tamil Nadu 3. Status of the Institute: Private Research Institute 4. Name and designation of the Executive Authority of the Institute/University forwarding the application: Dr. P. Radhakrishnan, Director, PSG Institute of Advanced Studies 5. Project Title: Development of gold nanoparticle based electrochemical immunosensor for snake venom detection using chicken egg yolk antisnake venom antibodies (IgY). 6. Category of the Project (Please tick): R&D / Programme Support 7. Specific Area (Please see Annexure - II): 2.8 Medical Sciences: vaccines and diagnosis 8. Duration: 3 Years 9. Total Cost (Rs): Rs 28,17, 500.00 10. Is the project Single Institutional or Multiple-Institutional (S/M)?: M 11. If the project is multi-institutional, please furnish the following: Name of Project Coordinator: Dr.R.Selvakumar Affiliation: Nanotech Research Facility, PSG Institute of Advanced Studies, Coimbatore-641004 Address: PSG Institute of Advanced Studies, Post Box No. 1609, Avinashi Road, Peelamedu, Coimbatore-641 004 12. Scope of application indicating anticipated product and processes In India, approximately 15000 people die every year due to snake bite and many cases remain unreported (Meenatchisundaram and Michael, 2009). Bites by non-poisonous
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1
PROFORMA – I
PROFORMA FOR SUBMISSION OF PROJECT PROPOSALS ON
RESEARCH AND DEVELOPMENT, PROGRAMME SUPPORT
(To be filled by the applicant)
PART I: GENERAL INFORMATION
1. Name of the Institute/University/Organisation submitting the Project Proposal:
PSG Institute of Advanced Studies,
Nanotechnology Research Facility,
Post Box No. 1609, Avinashi Road, Peelamedu,
Coimbatore-641 004
2. State: Tamil Nadu 3. Status of the Institute: Private Research Institute
4. Name and designation of the Executive Authority of the Institute/University
forwarding the application:
Dr. P. Radhakrishnan,
Director, PSG Institute of Advanced Studies
5. Project Title:
Development of gold nanoparticle based electrochemical immunosensor for
snake venom detection using chicken egg yolk antisnake venom antibodies
(IgY).
6. Category of the Project (Please tick): R&D / Programme Support
7. Specific Area (Please see Annexure - II):
2.8 Medical Sciences: vaccines and diagnosis
8. Duration: 3 Years
9. Total Cost (Rs): Rs 28,17, 500.00
10. Is the project Single Institutional or Multiple-Institutional (S/M)?: M
11. If the project is multi-institutional, please furnish the following:
Name of Project Coordinator: Dr.R.Selvakumar
Affiliation: Nanotech Research Facility, PSG Institute of Advanced Studies,
Coimbatore-641004
Address: PSG Institute of Advanced Studies, Post Box No. 1609, Avinashi
Road, Peelamedu, Coimbatore-641 004
12. Scope of application indicating anticipated product and processes
In India, approximately 15000 people die every year due to snake bite and many
cases remain unreported (Meenatchisundaram and Michael, 2009). Bites by non-poisonous
2
snakes are common and in these cases only mild to moderate local reactions are
observed. However, the signs, symptoms and prognosis in poisonous snake bite cases
depend upon the species and the type and quantity of venom injected. The venom of
cobras, kraits and coral snakes affect mainly the nervous system producing paralysis. The
venom of viper and pit viper act mainly on blood and body tissues, whereas venoms of
sea snakes are myotoxic. At present, after a snake bite, the physicians have to wait for
symptoms caused by envenoming due to lack of specific detection kit and forced to give
polyvalent polyclonal antivenom. The use of polyvalent polyclonal antivenom is not
recommended by WHO due to its hypersensitivity reaction. Hence in International
conference on snakes, venoms and snakebite (2008), Kerala, India, it was concluded that
diagnosis of snakebite is essential in treating envenomed patients. The increasing number
of deaths due to snake bite is due to lack of immediate medical treatment and detection of
the type of snake venom. Usually patients are administered with polyvalent snake venom
antibodies as a life saving medicine. Such polyvalent snake venoms will lead to the
development of many hypersensitive reactions in our body system. Thus immediate
detection of snake venom can be used to treat patient with particular monovalent
antibody. In most snake bite incidence, the patient retains no information on the type of
snake which has bitten. Hence development of biosensor to detect the possible toxin
present in the bitten site could help to treat patients with specific anti-snake venom
antibody instead of giving him a polyclonal antibody. Such treatment could enhance the
survival rate of patients and easy recovery. The out come of the proposed project will
help upto detect the quantity and type of venom in the blood/ tissue samples collected
from the patient. The proposal is aimed to detect highly sensitive electrochemical
biosensor for snake venom diction using nanobiotechnology method.
Project Summary
Snake bite remains a public health problem in many countries. It is estimated that
the incidence of snake envenomation could exceed 5 million per year (Chippaux, 1998). In
Asia, it has been estimated that a million snakebites occur each year, of which
approximately 50% are envenomed, resulting in 1,00,000 annual deaths. The snake venom
comprises of complex pool of proteins, organic and inorganic compounds. Among these
compounds, some enzymes like acetylcholine esterases, Adpase, phospholipase,
metalloprotease and serineproteinase leads to the toxicity of the venom. Among these
enzymes the metalloprotease and serineproteinase are haemostatic active components of
3
venom and differs from one species to another with widely varying toxicological features.
Polyvalent antivenom antibodies from pre-immunized horse sera are the only scope for the
doctors to neutralize venoms which have varying composition and type of haemostatic
active compounds. Avian yolk immunoglobulin (IgY) based antivenom had been first
suggested by Thally and Carrol (1990) to be less expensive, safer and more robust than
horse antivenoms. Many countries around the world claim great success in trials of IgY
antivenom for veterinary use. Dr.Michael and group developed similar IgY based
antibodies for various common poisonous snake in India (Meenatchisundaram et al.,
2009).
In this context, we are aiming to develop a gold nanoparticle based
electrochemical sensor for the detection of type of snake venom from the bitten site of the
patient. The proposed study will exploit the gold nanoparticles as electro active labels due
to its high stability, easy preparation, biological compatibility and excellent conductivity.
The gold nanoparticles will be tagged with biotinylated IgY antivenom (developed by
Dr.A.Michael, Co-PI) and coated onto glassy carbon electrode. A sandwich ELISA
method will be used for the reaction with venom in the sample. The electrochemical
signals produced will be analyzed using electrochemical impedance spectroscopy and
results will be interpreted. This study will help us to develop a specific electrochemical
biosensor for detection of low quantity venom in the blood/tissue samples and its type.
PART II: PARTICULARS OF INVESTIGATORS
Principal Investigator 1:
14 (a). Name: Dr.R.Selvakumar
Date of Birth: 24/05/1981 Sex (M/F): M
Designation: Assistant Professor
Department: Nanobiotechnology
Institute/University: PSG Institute of Advanced Studies
Address: Post Box No. 1609, Avinashi Road, Peelamedu, Coimbatore, Tamil
mg injected at the time of bite i.e much less as compare to fatal dose). But the amount of
venom neutralized by one ml of polyvalant ASV is known viz. cobra 0.6mg, Russell’s
viper 0.6mg, krait 0.45 and Echis carinatus 0.45 mg. Empirically total ASV required is
200, 250, 134 and 10.22 ml. respectively. However, the clinical features and outcomes
are not as simple as predicted, because every bite does not result in complete
envenomation. In India commercially produced ASV is relatively cheap as compared to
Western countries (Bawaskar, 2004). One vial of ASV costs Rs.400. Majority of snake
bite victims are reported to government hospitals because ASV is given free of cost, still
many victims delays hospitalization and vital time is killed by attending to Mantrik.
Paul et al (2003) reported an interesting finding of morbidity and mortality which is high
in group received high dose of ASV (120 ml) as against the group who received low dose
(60 ml) (Paul et al., 2004). Similar experience reported from Vellore and Rajastan
(Thomas and Jacob, 1985; Kothari et al., 2001). Kulkarni and Anees (1994) from
Karnataka studied 630 snake bite cases in pediatric and majority of his cases recovered
with 80 ml of ASV. Further reduction of requirement of ASV can be achieved by
encouraging ASV producers in India to prepare ASV from venoms obtained from snakes
caught from relevant areas of the country (Bhawaskar and Bhawaskar, 2002).
Manjula J. Kusum et al., (2006) generated antivenom specific antibodies in white
leghorn chicken and their egg yolks. They recently reported that the purity, efficacy and
ease of manufacture of avian antivenoms and their inability to fix mammalian
complement make them an attractive alternative to equine antivenoms. They also
specified that over 10-15 mg of venom specific antibodies can be obtained from an
immunized chicken's egg yolk. To our knowledge and search, we have not found any
publication for snake venom detection using electrochemical approach in India.
16.6 The relevance and expected outcome of the proposed study
The outcome of the proposed project will lead to the development of a technology
to differentiate venom proteins based on IgY antibody-nanoparticle interaction and will
ultimate lead to the development of a gold nanoparticle based electrochemical
immunosensor.
13
16.7 Preliminary work done so far
Preliminary work carried out by PI (Dr.R.Selvakumar)
Dr.R.Selvakumar has been working in the field of Nanobiotechnology at PSG
Institute of Advanced Studies since 2009. He has successfully synthesized many types of
nanoparticles using microorganism and have 4 publications on the same. At present,
citrate capped gold nanoparticles have been synthesized using chloroauric acid standard
reduction method and have been analyzed using HRTEM (Fig.1).
Fig. 1: Citrate capped gold nanoparticles synthesized at our laboratory
The impedance analysis of the gold nanoparticles were carried out using PARSTAT
impedance analyzer and its I-V characteristics were analyzed (fig 2 and 3).
Fig.2: impedance analysis of gold nanoparticles synthesized in our lab
14
Fig.3: I-V (i) and CV (ii) characteristics of gold nanoparticles synthesized in our laboratory Preliminary work carried out by Co- PI (Dr.A.Michael)
The Co-principal investigator has got extensive research experience in the field of
microbiology and immunology. He has been working on chicken antibodies from 1994
onwards. His Ph.D work itself was on immunodiagnosis of beta- haemolytic streptococci
using Chicken egg yolk antibodies as an alternative source to rabbit antibodies.
Through student project scheme he carried out research work on the generation of
antivenom in immunized chicken and their egg yolk. As a follow up study now 2 M.Phil
and 1 Ph.D., students of his are working on different aspects of antivenom generation in
Chicken. He is well versed in basic applied microbiological techniques, and specializes
in the field of medical microbiology, Immunology, Serology, Animal tissue culture
techniques, production of monoclonal and polyclonal antibodies and their
characterization immuno assays, purification and labelling of antibodies- Chicken egg
yolk antibodies production and their purification methods. During his research work he
underwent training/ participated in workshops in various prestigious institutions such as
National Institute of Immunology ( Total period 3 months ) WHO Collaborating Centre
for Streptococcal Diseases and Lady Hardinge Medical College, New Delhi, Christian
Medical College, Vellore, Indian Institute of Science and National Institute of Mental
Health and Neurosciences, Bangalore, Tuberculosis Research Centre, King Institute,
Cancer Institute, Center for Biotechnology - Anna University and Biotechnology Unit-
Veterinary University, Chennai to learn some techniques and hands on experience. He
was instrumental in bringing some immunological techniques to this centre. He has also
been one of the internal faculties involved in conducting of short term training programs
i) ii)
15
on basic microbiological techniques for college teachers and work shop on
immunotechnology conducted by our department.. He has so far guided 120 M.Sc
Applied Microbiology students project work and is at present guiding 40 M.Phil and 7
Ph.D students.
Preliminary work carried out by Co- I (Dr.P.Rani)
The co-investigator (Dr.P.Rani) has got extensive research experience in the field
of Biosensors and is working on development of diagnostic marker kit for the early
detection of Alzheimer’s Disease sponsored by DBT. She has successfully completed a
project on development of biosensor for water and air quality measurement funded by
DRDO.
References: 1. Chippaux JP (1998) Bull WHO. 76, 515- 524. 2. S. Meenatchisundaram & A. Michael, Ind.J. Sci Technol . 2(10) 69-73. 3. Maung-Maung-Thwin, P. Gopalakrishnakone, R. Yuen, C. H. Tan (1996) Toxicon, 34(2) 183-199. 4. L.Dong , L. K. Quyen , K.H.Eng , P. Gopalakrishnakone, (2003) J. Immuno. Methods. 282;13–3. 5. Paul V, Pratibha, Prahalad KS , Eraly J, Francis S, Lewis F. JAPI 2004;52:14-7. 6. Kothari D, Bomb BS, Bolya YK, Srivastva S, et al. JAPI 2001;49:57 7. Kulkarni ML, Anees Ind Pedtr 1994;31:1239-43. 8. Bawaskar HS, Bawaskar PH. Lancet 2002;360:1703. 9. Manjula J. Kusum.P, Sairam A.K, Murthy P.B. & Subbarao .P.V. Journal of Cell and Tissue Research Vol.6(2): 733-738.2006. 10. Thomas PP and Jacob J. BMJ1985;291:177-78. 11. Paul V, Pratibha, Prahalad KS , Eraly J, Francis S, Lewis F.JAPI. 2004;52:14-7. 12. Bawaskar HS, JAPI•VOL. 52•JANUARY 2004.11-13. 13. Paul V, Prahld KA , Earali J, Francis S, Lewis F. JAPI 2003;51:163-66. 14. LaIloo DG et al. Royal Society Tropical Medicine and Hygiene, 1995, 89: 178- 182. 15 Theakston et al., Joumal of tropical medicine and 84:hygiene, 199-202. 1981. 16. Schade,R., Calzado,E.G., Sarmiento,R., Chacana,P.A., Porankiewicz- Asplund,J., Terzolo,H.R.,2005. Altern.Lab.Anim.33,129–154. 17. Shimitzu,M.,Fitzsimmons,R.C.,Nakai,S.,1998. J.FoodSci.53,1360–1366. 18. Svendsen,B.,Hau,J.,1996. Scand.J.Lab.Ann.Sci.23,85–91. 19. Hadge,D.,Anbrosuius,H.,1984..Mol.Immunol.21,699–707. 20. Larsson,A.,Lindahl,T.,1993. Avian Immunology in Progress. INRA, Paris, pp.97– 102. 21. Wooley, J.A.,Landon,J.,1995. J. Immunol. Methods178, 253–265. 17. Specific objectives
To develop egg yolk antibodies (IgY) against purified venom in chicken Purify IgY antibodies based on affinity chromatography and to study its
immunological interactions with native venom of different snakes.
16
Studies on cross reactivity of anti-IgY antibodies with other components of the venom.
Liganding of IgY antibodies to noble metal nanoparticles and studies on change in electrochemical and optical properties using impedance spectroscopy, ELISA reader and other suitable techniques.
Quantitative and qualitative analysis of the IgY tagged noble metal nanoparticles for its specificity in interaction with various haemostatic proteinase compounds
Studying the possible application of the mechanism of antigen bounded antibody interaction with noble metal nanoparticles and glassy carbon electrode for electrochemical based biosensor development.
18. Work Plan: should not exceed 3-4 pages (the section can be divided according to the specific aims and under each specific aim, the following should be stated clearly as sub headings) 18.1 Work plan (methodology/experimental design to accomplish the stated
aim)
Over all experimental design:
Affinity based purification of IgY
Purified IgY antibodies
venom
Antigen injection
Healthy chickens
Anti venom IgY
Venom-antivenom IgY interaction studies
IgY liganding
Nobel metal nanoparticles
Nanoparticle coated Silicon/quartz substrate
Silicon/quartz substrate Electrode
Venom
Change in Impedance/ optical property
Application in biosensor
17
Detailed experimental design for biosensor development:
Glassy carbon electrode
Connectivity of the participating institutions and investigators (in case of
multi-institutional projects only)
Three major institutions (PSG Institute of Advanced Studies (PSG IAS),
PSG College of Arts and Science(PSG CAS) and PSG College of Technology
(PSG CT) will participate in the proposed research. PSG institutions, one of the
pioneers in the field of education for more than seven decades are committed to
Arrangement of Streptavidin conjugated gold nanoparticles onto electrodes
Biotinylated Purified IgY snake venom antibodies
Venom from bitten site/blood
Gold conjugate having IgY snake venom antibodies
Electrochemical impedance analysis
Addition of 0.1 mol/L HCl to electro-oxidized AuNPs to produce AuCl4-
18
provide world class engineering and science education through UG, PG and
research programs. They are well recognized by industry and R&D institutions.
PSG institutions have the reputation of being among the top institutions imparting
high quality education and have excellent industry-academia interaction.
The PIs will develop gold nanoparticles and tag with antibodies using the
facilities at PSG IAS. The Physical and biological characterization of such tagges
nanoparticles will be characterized at PSGIAS by the PI. The Co- PI
(Dr.A.Michael) from PSGCAS will take care on the isolation and purification of
IgY antibodies from egg yolk and provide for the experiment. The Co-I (Dr. P.
Rani) will take part in enhancing the sensitivity of the experiment by finding
appropriate system that will increase the signal yield for impedance analysis.
This work will be carried out at PSGCT. The facilities available in all the three
institutes will be used by the investigators.
18.3 Alternate strategies (if the proposed experimental design or method does
not work what is the alternate strategy)
The immuno fluorescence approach will be considered (as per the
following experimental design) as an alternative if the proposed technique doesn’t
work.
Streptavidin treated glass matrix
Biotinylated Purified IgY snake venom antibodies
Venom from bitten site/blood
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19. Timelines: (Please provide quantifiable outputs)
Period of
study
Achievable targets
6 Months Literature review, purchasing chemicals and
Appointment of JRF
12 Months Preparation and characterization of nanoparticles,
immunization of chicken and IgY production
18 Months Purification of IgY, Immunological characterization,
Optimization of conditions for IgY- gold nanoparticle
conjugation and deposition on electrode
24 Months Preparation of immunosensor and analysis using
electrochemical impedance spectroscopy.
30 Months Testing of biosensors for sensitivity and selectivity
36 Months Documentation, publication and review
CdSe quantum dot conjugate having IgY snake venom antibodies
Immuno fluorescence analysis using microscope
20
20. Name and address of 5 experts in the field
S.No Name Designation Address
1 Dr. S.K.Gupta Staff Scientist -VI & Head
Department of Gamete antigen laboratory, National Institute of Immunology, Aruna Asaf Ali marg, New Delhi -110 067. Ph: 6162281, 6188306,6183004 Fax :6162125/6177626.
2 Dr. G. Dhinakar Raj Professor
Department of Biotechnology, TamilNadu Veterinary and Animal Sciences University, Madras Veterinary College, Vepery, Chennai-600 007.Ph:5381506, 8274055(Res). Fax: 5381886,
Microscope - Phase contrast and Dark field Carl-zeiss
Microscope- Bright Field Olympus
Millipore water system - RO and Milli Q Imported
Minigel Electrophoresis system Broviga
Gas liquid chromatography Chemito
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2. Other resources such as clinical material, animal house facility, glass house,
experimental garden, pilot plant facility etc.
CPCSEA approved central animal house facilities are also available. Rodents,
non rodents and sheep are available as experimental animals. One operation
theatre with all necessary facilities to carry out surgery in smaller animals is
available in the animal house.
29
PART VI: DECLARATION/CERTIFICATION It is certified that
a) the research work proposed in the scheme/project does not in any way duplicate the work already done or being carried out elsewhere on the subject.
b) the same project proposal has not been submitted to any other agency for financial support.
c) the emoluments for the manpower proposed are those admissible to persons of corresponding status employed in the institute/university or as per the Ministry of Science & Technology guidelines (Annexure-III)
d) necessary provision for the scheme/project will be made in the Institute/University/State budget in anticipation of the sanction of the scheme/project.
e) if the project involves the utilisation of genetically engineered organisms, we agree to submit an application through our Institutional Biosafety Committee. We also declare that while conducting experiments, the Biosafety Guidelines of the Department of Biotechnology would be followed in toto.
f) if the project involves field trials/experiments/exchange of specimens, etc. we will ensure that ethical clearances would be taken from concerned ethical Committees/Competent authorities and the same would be conveyed to the Department of Biotechnology before implementing the project.
g) it is agreed that any research outcome or intellectual property right(s) on the invention(s) arising out of the project shall be taken in accordance with the instructions issued with the approval of the Ministry of Finance, Department of Expenditure, as contained in Annexure-V.
h) we agree to accept the terms and conditions as enclosed in Annexure-IV. The same is signed and enclosed.
i) the institute/university agrees that the equipment, other basic facilities and such other administrative facilities as per terms and conditions of the grant will be extended to investigator(s) throughout the duration of the project.
j) the Institute assumes to undertake the financial and other management responsibilities of the project. Signature of Project Coordinator Signature of Executive Authority (Dr.R.Selvakumar) of Institute/University with seal Date : Date : Signature of Principal Investigator: Signature of Co-PI: (Dr.R.Selvakumar) (Dr.A.Michael) Date: Date: Signature of Co-I (Dr.P.Rani) Date:
30
PART VII: PROFORMA FOR BIOGRAPHICAL SKETCH OF INVESTIGATORS
Provide the following information for the key personnel in the order listed on PART II.
Follow this format for each person. DO NOT EXCEED THREE PAGES Name: Dr. R.Selvakumar Designation: Assistant Professor in Nanobiotechnology Department/Institute/University: PSG Institute of Advanced Studies, Peelamedu, Coimbatore - 4 Date of Birth: 24/05/1981 Sex (M/F): M SC/ST: Not applicable Education: (Post-Graduation onwards & Professional Career)
S. No.
Institution Place
Degree Awarded
Year Field of Study
1 Bharathiar University
M.Sc. 2003 Applied Microbiology
2 Bharathiar University
Ph.D. 2009 Biotechnology
A. Position and Honors Position and Employment (Starting with the most recent employment)
Sl No.
Institution Place
Position From (Date)
To (date)
1 PSG Institute of Advanced studies, Coimbatore, India
Assistant Professor
June 2009
Till date
Honors/Awards:
Received DST-International Travel Grant for attending Asia Nano2008 conference at Singapore
Received Certificate of Recognition from Vice Chancellor and Syndicate of Bharathiar University for presenting paper at Asia Nano2008 conference at Singapore
CSIR-SRF (Senior Research Fellow) awarded by Council for Scientific and Industrial Research, Government of India.
Senior Research Fellow awarded by Defence Research and Developmental Organization, Government of India.
Junior Research Fellow awarded by Defence Research and Developmental Organization, Government of India.
Received Academic Award for Distinction in B.Sc., Microbiology.
31
Professional Experience and Training relevant to the Project:
I have 2.5 years of research experience in the synthesis of biological and chemical nanoparticle and in Nanobiotechnology. From the date of joining I have been working on development of biosensor for cancer marker detection for which I have been granted a minor project worth Rs 25,000 by Villgrow-PSG. Three B.tech students are doing research project under me for the development of above mentioned sensor. Myself and Dr. Rani (Co-Investigator) are working for the development of visual detection kit for snake venom using immunosensor method. B. Publications: 12 (Peer reviewed, International: 11 and National: 1)
Selected peer-reviewed publications (Ten best publications in chronological order):
1. R.Selvakumar, K.Karthikeyan and P.Radhakrishnan. 2011. Analysis on surface nanostructures present in hindwing of dragon fly (Sympetrum vulgatum) using atomic force microscopy. Micron (Accepted). (Selected for special issue on “Atomic Force Microscopy in Biology & Biomedicine” in Micron) (SCI indexed; impact factor: 1.649)
2. R.Selvakumar, S.Aravindh, C.P.Kaushik, V.G.Katarani, Vidya.S.Thorat, Prema Gireesan, V. Jayavignesh, K. Swaminathan and Kanwar Raj, 2011. Screening of silver nanoparticles containing carbonized yeast cells for adsorption of few long- lived active radionuclides. Journal of Radioanalytical and Nuclear Chemistry. 288:629–633. (SCI indexed; impact factor: 0.777)
3. R.Selvakumar, N. Arul Jothi, V. Jayavignesh, K. Karthikaiselvi, Geny Immanual Antony, P.R. Sharmila, S.Kavitha and K. Swaminathan. 2011. As(V) removal using carbonized yeast cells containing silver nanoparticles, Water Research, 45: 583-592 (SCI indexed; impact factor: 4.546)
4. R.Selvakumar, S.Kavitha, M.Sathishkumar, V.Jayavignesh and K.Swaminathan. 2010. Liquid phase separation of As(V) from aqueous solution using pretreated
5. S.Kavitha, R.Selvakumar, M.Sathishkumar, K.Swaminathan, P.Lakshmanaperumalsamy, A.Singh and S.K.Jain. 2009. Nitrate removal using ‘ Brevundimonas diminuta MTCC 8486 from ground waters. Water Science and Technology, 60(2): 517–524. (SCI indexed; impact factor: 1.056)
6. M. Sathishkumar, A.R. Binupriya, D. Kavitha, R. Selvakumar, R. Jayabalan, S.E.Yun. 2009. Adsorption potential of maize cob carbon for 2, 4-dichlorophenol removal from aqueous solutions: Equilibrium, kinetics and thermodynamics
modeling. Journal of Chemical Engineering, 147: 265-271 (SCI indexed; impact factor: 3.074)
32
7. R.Selvakumar, S.Kavitha, M.Sathishkumar and K.Swaminathan. 2008. “Arsenic adsorption by polyvinyl pyrrolidone K25 coated cassava peel carbon from aqueous solution” Journal of Hazardous Materials, 153(1-2): 67-74. (SCI indexed; impact factor: 3.723)
8. S. Kavitha, R. Selvakumar and K. Swaminathan. 2008. As(V) adsorption onto Polyvinyl pyrrolidone K25 doped pretreated Aspergillus clavatus biomass from
S.E.Yun and K.Swaminathan. 2008. Organic micropollutant removal in liquid phase using carbonized silk cotton hull. Journal of Environmental Sciences, 20(9):
1046-1054. (SCI indexed; impact factor: 1.513)
10. R.Selvakumar, S.Kavitha and K.Swaminathan. 2007. Adsorption of As(v) from aqueous solution by chemically doped coir pith carbon, Indian Journal of Chemical Technology. 14: 276-282. (SCI indexed; impact factor: 0.373) List maximum of five recent publications relevant to the proposed area of work:
1. R.Selvakumar, S.Aravindh, C.P.Kaushik, V.G.Katarani, Vidya.S.Thorat, Prema Gireesan, V. Jayavignesh, K. Swaminathan and Kanwar Raj, 2011. Screening of silver nanoparticles containing carbonized yeast cells for adsorption of few long- lived active radionuclides. Journal of Radioanalytical and Nuclear Chemistry. 288:629–633. (SCI indexed; impact factor: 0.777)
2. R.Selvakumar, N. Arul Jothi, V. Jayavignesh, K. Karthikaiselvi, Geny Immanual Antony, P.R. Sharmila, S.Kavitha and K. Swaminathan. 2011. As(V) removal using carbonized yeast cells containing silver nanoparticles, Water Research, 45: 583-592 (SCI indexed; impact factor: 4.546) Place: Coimbatore Signature of Investigator Date:
33
PART VII: PROFORMA FOR BIOGRAPHICAL SKETCH OF INVESTIGATORS
Provide the following information for the key personnel in the order listed on
PART II. Follow this format for each person. DO NOT EXCEED THREE PAGES
Name: Dr. A. MICHAEL Designation: Associate Professor and Head, Dept. of Microbiology
Department/Institute/University: PSG College of Arts and Science Date of Birth: 21/ 5/ 1962 Sex (M/F): M SC/ST: Not applicable Education: (Post-Graduation onwards & Professional Career) Sl No. Institution Place Degree
Awarded Year Field of Study
1 PSG College of Arts & Science, Coimbatore
M. Sc 1986
Applied Microbiology
2 Voluntary Health Services, Chennai
Diploma in Medical technology
1987 Medical technology
3
Bharathiar University, Coimbatore
Ph. D. 1999
Microbiology
4 Bharathiar University, Coimbatore
D.Sc. 2011( registered) Microbiology
A. Position and Honors: Position and Employment (Starting with the most recent employment)
S. No.
Institution Place
Position From (Date)
To (date)
1 PSG College of Arts and
Science, Coimbatore
Associate Professor and Head, Dept. of Microbiology
1.1.2007 Till date
2 PSG College of Arts and Science, Coimbatore
Reader and Head, Dept. of
Microbiology 24.7.2006 31.12.2006
3 PSG College of Arts and Science, Coimbatore
Reader in Microbiology 25.10.1999 24.7.2005
4 PSG College of Arts and
Science, Coimbatore
Selection Grade Lecturer in
Microbiology 21.9.1998 25.10.1999
5 PSG College of Arts and Science, Coimbatore
Senior Lecturer in Microbiology 20.9.1993 21.9.1998
34
Honors/Awards:
1. Awarded ‘PSG Management Institutional Gold Medal’ for the excellent performance on my Ph.D. Research work on “Immunodiagnostics of Group A Streptococci by latex agglutination and coagglutination assay with polyvalent chicken and rabbit antibodies” for the academic year 2000-2001.
2. Awarded ‘PSG Management Institutional Gold Medal’ for excellence in
Research for the years 2000, 2002 and 2003.
3. Awarded ‘Outstanding Teacher -2010’ for my contribution to the teaching and promotion of research in Applied Microbiology at 8th Annual Conference of IAAM held at Bharathidasan University, Tiruchirappalli, September 2010.
4. Recently awarded ‘Life Time Achievement Award’ for my 25 years of teaching
and contribution to Microbiology at 9th Annual Conference of IAAM held at Periyar University, Salem on 14th and 15th October 2011.
5. I got the best paper award along with Dr.N.Kannan and Dr.B. Appalaraju for my
work on Chicken antibodies as an alternative source for serodiagnosis of streptococcal isolates during the XXIV National Congress of Indian Association of Medical Microbiologists, held from 17.11.2000 to 19.11.2000 at Belgaum, Karnataka.
Professional Experience and Training relevant to the Project:
I got my Doctorate degree on “Immonodiagnosis of Group – A streptococci by latex agglutination and coagglutination assay with polyvalent chicken and rabbit antibodies”. I was always interested in alternative methods of diagnosis and treatment of infectious diseases. I have been working on Chicken egg yolk antibodies since 1998. We have generated antibodies in chicken against various bacterial, viral, fungal infectious agents and against Indian Poisonous Snake Venoms. Producing antibodies in laying hens is commercially feasible, because the eggs from immunized chicken provide a continuous daily source of polyclonal antibodies and this convenient approach offers greater compatibility with animal protection regulation. With my experience in the field of antibody engineering, I would like to develop a Center of Excellence in Immunotechnology, which should be a national facility. It would be my contribution towards the development of our country.
B. Publications: 22 (Peer reviewed, International: 16 and National: 6)
Selected peer-reviewed publications (Ten best publications in chronological order)
1. 1. S.Meenatchisundaram, G.Parameswari, Michael A and S.Ramalingam.“Studies on pharmacological effects of Russell’s viper and Saw-scaled viper venom and its Neutralization by chicken egg yolk antibodies” - International immunopharmacology journal. 2008; 8:1067–1073.
2. S.Meenatchisundaram, G.Parameswari, Michael A and S.Ramalingam.
“Neutralization of pharmacological effects of cobra and krait venom by chicken egg yolk antibodies’ Toxicon. 2008; 52:221–227.
3. B.Muralikrishnan, S.Shajith Anoop, K.Karthikeyan, K.Nanthakumar and
Michael A. Comparative studies on generation of anti-venom in chicken using bentonite and adjuvant coated venoms of common venomous snakes in India. African Journal of Microbiology Research. 2009. Vol 3(7).
4. Meenatchisundaram. S, Priyagrace. S, Vijayaraghavan. R, Velmurugan. A,
Parameswari. G and Michael A. Antitoxin activity of Mimosa pudica root extracts against Naja naja and Bangarus caerulus venom, A Journal of the Bangladesh Pharmacological Society (BDPS), 2009; 4: 105-109
5. Meenatchisundaram. S and Michael A. Preliminary Studies on Antivenom Activity of Mimosa Pudica Root Extracts against Russell’s viper and Saw Scaled Viper Venom By In Vivo and In Vitro Methods. Pharmacologyonline. 2009; 2: 372-378.
6. S.Meenatchisundaram, R.Selvakumaran, G.Parameswari and Michael A. Comparison of Antivenom Potential of Chicken Egg Yolk Antibodies Generated against Betonite and Adjuvent Coated Venoms of Common Poisonous Snake in India, Bangl. J. Vet. Med. 2009; 7(1): 259-267.
7. S. Meenatchisundaram and Michael A. Comparison of four different purification
methods for isolation of anti Echis carinatus antivenom antibodies from immunized chicken egg yolk, Iranian Journal of Biotechnology, 2010; Vol.8, No. 1.
8. S.Meenatchisundaram and Michael A., Antitoxin activity of Mucuna pruriens aqueous extracts against Cobra and Krait venom by in vivo and in vitro methods, International Journal of PharmTech Research, 2010; Vol.2, No.1: pp 870-874.
9. Meenatchisundaram.S Michael A. T.Subbraj, T.Diraviam, and V.Shanmugam, Isolation, Purification and Neutralizing potential of chicken egg yolk immunoglobulin (IgY) against mastitis causing Escherichia coli in dairy cows in Coimbatore District, International Journal of Drug Development & Research. 2011; Vol. 3(2):147-153.
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10. Sentila, R., Karthika, S., and Michael A. 2011. Generation of egg yolk antibodies in chicken (IgY) against Streptococcus mutans and its in-vitro neutralization efficacy. Archives of applied science, 3(5):404-412.
List maximum of five recent publications relevant to the proposed area of work:
1. S.Meenatchisundaram, R.Selvakumaran, G.Parameswari and Michael A. Comparison of Antivenom Potential of Chicken Egg Yolk Antibodies Generated against Betonite and Adjuvent Coated Venoms of Common Poisonous Snake in India, Bangl. J. Vet. Med. 2009; 7(1): 259-267.
2. S. Meenatchisundaram and Michael A. Comparison of four different purification
methods for isolation of anti Echis carinatus antivenom antibodies from immunized chicken egg yolk, Iranian Journal of Biotechnology, 2010; Vol.8, No. 1.
3. S.Meenatchisundaram and Michael A., Antitoxin activity of Mucuna pruriens aqueous extracts against Cobra and Krait venom by in vivo and in vitro methods, International Journal of PharmTech Research, 2010; Vol.2, No.1: pp 870-874.
4. Meenatchisundaram.S Michael A. T.Subbraj, T.Diraviam, and V.Shanmugam, Isolation, Purification and Neutralizing potential of chicken egg yolk immunoglobulin (IgY) against mastitis causing Escherichia coli in dairy cows in Coimbatore District, International Journal of Drug Development & Research. 2011; Vol. 3(2):147-153.
5. Sentila, R., Karthika, S., and Michael A. 2011. Generation of egg yolk antibodies in chicken (IgY) against Streptococcus mutans and its in-vitro neutralization efficacy. Archives of applied science, 3(5):404-412.
Place: Coimbatore Signature of Investigator Date:
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PART VII: PROFORMA FOR BIOGRAPHICAL SKETCH OF INVESTIGATORS
Provide the following information for the key personnel in the order listed on PART II.
Follow this format for each person. DO NOT EXCEED THREE PAGES Name: Dr. P. Rani
Designation: Associate Professor Department/Institute/University: Department of Biotechnology, PSG College of Technology Date of Birth: 10.04.1965 Sex (M/F): M SC/ST: Not applicable
Education: (Post-Graduation onwards & Professional Career) Sl No. Institution
Place Degree Awarded
Year Field of Study
1 Bharathiar University, Coimbatore
M. Sc. 1988 Biochemistry
2 Avinashilingam Deemed University, Coimbatore
M.Phil 1989 Biochemistry
3 Indian Institute of Technology, Chennai
Ph.D 1996 Selenium Biochemistry
B. Position and Honors: Position and Employment (Starting with the most recent employment)
Sl No.
Institution Place
Position From (Date)
To (date)
1 Department of Biotechnology, PSG College of Technology
Assistant Professor
2004 Till date
2 Department of Biotechnology, PSG College of Technology
Lecturer, 2001 2003
3 Dept of Biotechnology, Kongunadu College of Arts and Science
Lecturer, 1996 2001
Honors/Awards:
Recipient of National Merit Scholarship from 1981-86 First prize for proficiency in M.Sc Recipient of old Medal in M.Sc Best poster presentation at the Society of Biological Chemists (I) held in
Calcutta, 1991 JRF and SRF from Indian Institute of Technology, Madras (1990- 1995) CSIR Research Associate Fellowship (1995-1996) Best paper presentation- International Conference on New Horizons in
Biotechnology, Nov 2007 BRSI-NIIST, Trivandrum, India
Professional Experience and Training relevant to the Project: I have been granted with two project for the development of biosensors for water and air quality measurement (funded by DRDO) and an ongoing project on “Development of
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diagnostic marker kit for the early detection of Alzheimer’s Disease” (Funded by DBT). Other than the above I have experience in the following areas
Development of ELIZA based immunosensors Mammalian selenoproteins characterization Characterization of plant antioxidants Oxidative damage and neurological disorders Biotechnology based extension service to rural community development
B. Publications: 12 (Peer reviewed, International: 5 and National:7)
Selected peer-reviewed publications (Ten best publications in chronological order): 1. Evaluation of lignocellulosic wastes for production of edible mushrooms P. Rani, N. Kalyani and K. Prathiba Applied Biochemistry and Biotechnology, 151, 151-159, 2008
2. Evaluation of antioxidant properties of Berries P.Rani, K.Meena Unni and J.Karthikeyan Indian Journal of Clinical Biochemistry, 19(2), 117 - 125, 2004
3. Enzymatic and non-enzymatic antioxidants in selected Piper species J.Karthikeyan and P.Rani Indian Journal of Experimental Biology 41(2) , 135-140, 2003
4. Structural and thermodynamic consequences of introducing -aminoisobutric acid in the S- peptide of ribonuclease S Girish. S. Ratnaparkhi, Satish Kumar Awasthi, P.Rani, P.Balaram and R.Varadarajan Protein Engineering, 13(10) , 697 -702, 2000
5. Evidence for Altered Structure and Impaired Mitochondrial Electron Transport Function in Selenium Deficiency P.Rani, and K.Lalitha Biological Trace Element Research, 51 (3), 225-234, 1996.
6. Mitochondrial Selenium – 75 uptake and Regulation Revealed by Kinetic Analysis K.Lalitha, and P.Rani Biological Trace Element Research, 49(1), 21-42,1995.
7. Metabolic Relevance of Selenium in the Insect Corcyra cephalonica – Uptake of 75 Se and Subcellular Distribution K.Lalitha, P.Rani, and V.Narayanaswami Biological Trace Element Research, 41(3),217-233,1994. List maximum of five recent publications relevant to the proposed area of work: Nil Place: Coimbatore Signature of Investigator Date: