Nungky Kescandra 20100310122 Faculty of Medicine and Health Science Muhammadiyah University of Yogyakarta Correlation Between Hypoxia Inducible Factor (HIF)-1α Expression with Cell Death in Colorectal Cancer
Dec 08, 2015
Nungky Kescandra20100310122
Faculty of Medicine and Health ScienceMuhammadiyah University of Yogyakarta
Correlation Between Hypoxia Inducible Factor (HIF)-1α Expression with Cell Death in Colorectal
Cancer
Colorectal cancer is the 3rd most common cancer in men and the second in women worldwide epidemiology in 2008.
World 1.234.000 incidences with the mortality about 608.000 (IARC, 2008).
Indonesia in 2008, there were about 33.379 incidences with mortality range around 25.253 (IARC, 2008).
Background
TUMOR
HYPOXIA
HIF-1α
NEKROSIS
Problem Definition
What is the relation between Hypoxia Inducible Factor (HIF)-1α with cell death in
colorectal cancer?
Literature Review
Nature.com
Pathophysiology of Cancer
Colorectal Cancer
Polyp Colorectal
Mucosa
Polyp hyperplasia
Polyp adenomatous
Robbins & kumar Buku Ajar Patologi
Staging of Colorectal Cancer http://www.hopkinscoloncancercenter.org/
Hypoxia-Inducible Factor (HIF)-1α is a protein that made of helix loop -heliks-Per-Arnt-Sim (bHLH-PAS) substances
Protein ini merupakan penentu dari bentuk dan aktivitas transkripsi dari HIF-1 yang merupakan pengatur utama dari homeostasis oksigen dan berperan penting dalam proses fisiologi dan patologi adaptasi terhadap tekanan oksigen rendah (hipoksia).
HIF-1α
Regulation of HIF-1α
Semenza, 2003
(Semenza, 2003)
Apoptosis : the death of a cell that already has been programmed and also has such an ability to suicide itself through various cellular signals pathways. Apoptosis didn’t cause inflammation and didn’t harm another cells around (Edinger, & Thompson, 2004).
Nekrosis : the death of a cell that unprogrammed, in pathologic ways, and believed as trauma or hypoxia condition result and irreversible (Zong, & Thompson., 2006).
Cell Death
There is relation between HIF-1α expression with cell death in colorectal cancer
Hypothesis
RESEARCH METHODOLOGY
Research Design
Research Subject
cross sectional Non-experimental
patients are examined or transmit the colorectomy to Cito Pathology Laboratory in January 2011 until May 2012.
Adenocarcinoma (operation) and another Pathological Anatomy examination from january 2011 – may 2012Adenocarcinoma Biopsy
Inclusion Criteria
Exclusion Criteria
Variable
Variable Independent:
HIF-1α
Variable Dependent:
Cell Death
Nominal
Nominal
Rumus Sampel
Work Procedures
Microscopic
HEStaining
IHCStaining
Analysis
Data Analysis
Data yang dikumpulkan kemudian diuji menggunakan Chi-Square .
Thank You