Pöschl et al., Suppl. Mater Supplementary Material: Suppl. Figure 1: Related to Figure 1. Details on the 423 analyzed human medulloblastoma samples. Suppl. Figure 2: Related to Figure 1. The top 25 upregulated genes per human medulloblastoma subgroup out of the differentially expressed genes used for comparative analyses of human and murine data. Suppl. Figure 3: Related to Figure 1. Mouse Wnt Medulloblastomas model well human WNT medulloblastomas, independent of TP53 mutation status. Suppl. Figure 4: Related to Figure 2. Human SHH-medulloblastomas form a „infant“ and “adult“ cluster, but no distinct “childhood“ cluster. Suppl. Figure 5: Related to Figure 2. Validation of significantly differentially expressed genes between infant and adult human SHH-medulloblastomas, that were used for analysis. Suppl. Figure 6: Related to Figure 2. Heatmap showing 287 similarly expressed genes of adult human SHH medulloblastoma and murine Shh medulloblastoma mouse models Suppl. Table 1: Related to Figure 1. Raw AGDEX values and Perm P values shown in Figure 1A. Suppl. Table 2: Related to Figure 2. Raw AGDEX values and Perm P values shown in Figure 2A. Suppl. Table 3: Related to Figure 2 and Supplementary Figure 6. List of the 287 similarly expressed genes of adult human SHH medulloblastoma and murine Shh medulloblastoma mouse models.
Pöschl et al., Suppl . Material. Supplementary Material: Suppl . Figure 1: Related to Figure 1. Details on the 423 analyzed human medulloblastoma samples. Suppl . Figure 2: Related to Figure 1. - PowerPoint PPT Presentation
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Pöschl et al., Suppl. Material
Supplementary Material:
Suppl. Figure 1: Related to Figure 1. Details on the 423 analyzed human
medulloblastoma samples.
Suppl. Figure 2: Related to Figure 1. The top 25 upregulated genes per human
medulloblastoma subgroup out of the differentially expressed genes used for comparative analyses of human and murine data.
Suppl. Figure 3: Related to Figure 1. Mouse Wnt Medulloblastomas model well
human WNT medulloblastomas, independent of TP53 mutation status.
Suppl. Figure 4: Related to Figure 2. Human SHH-medulloblastomas form a
„infant“ and “adult“ cluster, but no distinct “childhood“ cluster.
Suppl. Figure 5: Related to Figure 2. Validation of significantly differentially
expressed genes between infant and adult human SHH-medulloblastomas, that were used for analysis.
Suppl. Figure 6: Related to Figure 2.Heatmap showing 287 similarly expressed
genes of adult human SHH medulloblastoma and murine Shh medulloblastoma mouse
models
Suppl. Table 1: Related to Figure 1. Raw AGDEX values and Perm P values
shown in Figure 1A.
Suppl. Table 2: Related to Figure 2. Raw AGDEX values and Perm P values
shown in Figure 2A.
Suppl. Table 3: Related to Figure 2 and Supplementary Figure 6. List of the 287 similarly expressed genes of
adult human SHH medulloblastoma and murine Shh medulloblastoma mouse models.
0 100 200 3000
50
100WNTSHHGroup 3Group 4
Time [months]
Ove
rall
surv
ival
[%]
Supplementary Figure 1: Epidemiological and clinical data of the 423 analyzed human medulloblastoma samples.
Pöschl et al., Suppl. Figure 1
Molecular subgroup n [% of all patients] 53 [12.53] 112 [26.48] 94 [22.22] 164 [38.77]
Agegroup n % of subgroup n % of subgroup n % of subgroup n % of subgroupInfant (≤ 3 years) 0 0 29 25.89 25 26.6 8 4.88
Not available 2 1.79 3 3.19 3 1.83Gender n % of subgroup n % of subgroup n % of subgroup n % of subgroupFemale 28 52.83 53 47.32 28 29.79 48 29.27Male 25 47.17 59 52.68 66 70.21 116 70.73
M stage n % of subgroup n % of subgroup n % of subgroup n % of subgroupM - 40 75.47 77 68.75 57 60.64 86 52.44M + 5 9.43 14 12.5 31 32.98 60 36.59
Not available 8 15.09 21 18.75 6 6.38 18 10.98Histology n % of subgroup n % of subgroup n % of subgroup n % of subgroupClassic 49 92.45 38 33.93 59 62.77 123 75
Not available 0 0 13 11.61 8 8.51 14 8.5410 year survival rate
WNT SHH Group 3 Group 4
97.8% 61.8% 43.5% 67.7%
WNT SHH Group 3 Group 4
Supplementary Figure 2: Heatmap showing the top 25 upregulated genes per human medulloblastoma subgroup. High expression is shown in red, low expression is shown in blue.
Pöschl et al., Suppl. Figure 2
data not available
TP53
TP53 mutated (n=4)TP53 WT (n=30)
Histology
Classic (n=34)
Gender
Female (n=20)Male (n=14)
Age group
Child (n=27)Adult (n=7)
Status
Alive (n=31)Dead (n=2)
Supplementary Figure 3A: Non-hierarchical average linkage clustering (20% variance filter) of 34 human WNT tumors with known TP53 mutation status. The four TP53 mutated WNT tumors did not cluster together.
TP53Status
HistologyGender
Age group
Pöschl et al., Suppl. Figure 3
Age group: Infant (≤ 3 years; n=29) Child (> 3 years < 16 years; n=27) Adult (≥ 16 years; n=54)
Supplementary Figure 4: Based on gene expression, SHH childhood medulloblastoma cases do not form a distinct cluster. Hierarchical clustering based on significantly differentially expressed genes using an “infant“, “childhood“ and “adult“ group distinguished an “infant“ and “adult“ cluster, but no distinct “childhood“ cluster. Instead childhood tumors clustered variably to the “infant“ or “adult“ SHH subgroup, with the majority been found in the „adult“ subgroup.
Pöschl et al., Suppl. Figure 4
Northcott dataset
200 (out of 332)
Current dataset
705101Age groupInfant (n=29)
Adult (n=54)
-2 0 +2
Supplementary Figure 5: 705 genes were found to be significantly differentially expressed between infantile and adult human SHH medulloblastomas in the current dataset (out of 10333 orthologue genes present on all examined platforms). The Venn diagramm shows the overlap of significant genes with the Northcott study (Northcott et al. 2011a). The Northcott study identified 332 significant differentially expressed genes – of these 200 were also present in our current dataset. 101 genes were found to be differentially expressed in both studies. Expression of these 101 genes in our dataset is displayed in the heatmap.
Pöschl et al., Suppl. Figure 5
-2 0 +1.5
SHH infant (n=29)
SHH adult (n=54)
Mouse modelsn=64
Supplementary Figure 6: Heatmap showing 287 signifcant differentially expressed genes when comparing human infant SHH medulloblastoma to human adult medulloblastoma and murine medulloblastoma models. Details on the genes can be found in table S3.
Pöschl et al., Suppl. Figure 6
Genotype WNT PERM P SHH PERM P Group 3 PERM P Group 4 PERM P
Supplementary Table 1: Raw AGDEX values and Perm P values when comparing different murine medulloblastoma models to the four subgroups of medulloblastoma. The best match for each model is highlighted in orange.
Pöschl et al., Suppl. Table 1
Genotype Tumor localisation INFANT PERM P ADULT PERM P
Supplementary Table 2: Raw AGDEX values and Perm P values when comparing different murine Shh medulloblastoma models to infantile or adult human SHH medulloblastoma cases. Best matches of each model are highlighted in orange.
Pöschl et al., Suppl. Table 2
Pöschl et al., Suppl. Table 3High in human infant SHH Tumors
Suppl. Table 3: 287 genes that are similarly expressed in adult human SHH medulloblastoma and murine Shh medulloblastoma mouse models and significantly differ from infantile human SHH medulloblastoma.