Photosynthetic Capacity in Photosynthetic Capacity in Coral Reef Systems: Coral Reef Systems: Applications for the Applications for the Underwater PAM Fluorometer Underwater PAM Fluorometer Adrian Jones & William Adrian Jones & William Dennison Dennison Thanks to the students of Coral Reef Biology & Thanks to the students of Coral Reef Biology & Geology (ID211) of 1996 and Terrestrial & Marine Geology (ID211) of 1996 and Terrestrial & Marine Environmental Physiology (BT230) of 1997 Environmental Physiology (BT230) of 1997 Marine Botany Marine Botany The University of Queensland The University of Queensland
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Photosynthetic Capacity in Coral Reef Systems: Applications for the Underwater PAM Fluorometer Adrian Jones & William Dennison Thanks to the students of.
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Photosynthetic Capacity in Coral Photosynthetic Capacity in Coral Reef Systems: Applications for the Reef Systems: Applications for the
Adrian Jones & William DennisonAdrian Jones & William Dennison
Thanks to the students of Coral Reef Biology & Geology (ID211) of Thanks to the students of Coral Reef Biology & Geology (ID211) of 1996 and Terrestrial & Marine Environmental Physiology (BT230) of 1996 and Terrestrial & Marine Environmental Physiology (BT230) of
19971997
Marine BotanyMarine BotanyThe University of QueenslandThe University of Queensland
AimsAims
• Generate photosynthesis versus irradiance (PI) Generate photosynthesis versus irradiance (PI) curves using Pulse Amplitude Modulated curves using Pulse Amplitude Modulated (PAM) fluorescence techniques (PAM) fluorescence techniques
• For a variety of marine macroalgae, determine For a variety of marine macroalgae, determine relationships between PI curves and various relationships between PI curves and various environmental factors environmental factors
• Use ecophysiological responses to infer light Use ecophysiological responses to infer light availability, desiccation stress and nutrient statusavailability, desiccation stress and nutrient status
PAM FluorometerPAM Fluorometer
Fibre OpticFibre OpticCableCable
Leaf ClipLeaf Clip
SubmersibleSubmersibleHousingHousing
• PAM generates saturating pulse of light which is used to PAM generates saturating pulse of light which is used to measure photosynthetic ratesmeasure photosynthetic rates
• Photosynthesis in Photosynthesis in ChlorodesmisChlorodesmis was measured along a was measured along a transect from 15m depth along the reef flat to the beachtransect from 15m depth along the reef flat to the beach
• Chlorodesmis Chlorodesmis collected from the reef flat and 15m collected from the reef flat and 15m was subjected to desiccation and fluorescence was monitored.
• Several species of macroalgae and coral Several species of macroalgae and coral were shaded and the change in fluorescence were shaded and the change in fluorescence measured over 5 days.measured over 5 days.
• Several species of macroalgae and coral were incubated Several species of macroalgae and coral were incubated for 10 days in flow-through aquaria with added for 10 days in flow-through aquaria with added nitrogen (88g mnitrogen (88g m-2-2) and phosphorus (22g m) and phosphorus (22g m-2-2) )
FertilisationsFertilisations
NutrientNutrientSufficiency StatusSufficiency Status
0
10
20
30
40
50
0 500 1000 1500 2000
FertilisedUnfertilised
PadinaPadina
ET
R (
µm
ol e
- m-2 s
-1 )
PAR (µmol quanta m-2 s-1)
0
5
10
15
20
25
30
0 200 400 600 800 1000 1200 1400
FertilisedUnfertilised
ChlorodesmisChlorodesmis
PAR (µmol quanta m-2 s-1)
0102030405060
0 500 1000 1500 2000
FertilisedUnfertilised
ColpomeniaColpomenia
ET
R (
µm
ol e
- m-2 s
-1 )
PAR (µmol quanta m-2 s-1)
FertilisationsFertilisations
0
20
40
60
80
100
0 500 1000 1500
FertilisedUnfertilised
AcroporaAcropora
PAR (µmol quanta m-2 s-1)
SummarySummary
• Rapid light curves in terrestrial and marine plants can be Rapid light curves in terrestrial and marine plants can be used to assess a variety of ecophysiological responsesused to assess a variety of ecophysiological responses
• Ability to generate Ability to generate in situ in situ PI curves rapidly, non PI curves rapidly, non destructively to determine relationships with various destructively to determine relationships with various environmental factorsenvironmental factors
• Ecophysiological responses to environmental gradients such Ecophysiological responses to environmental gradients such as desiccation, light, and depth can be ascertainedas desiccation, light, and depth can be ascertained
• PI responses can be used to infer a nutrient sufficiency status PI responses can be used to infer a nutrient sufficiency status