matter: towards improved in-situ fluorometer development ... · Fluorescence spectroscopic characterisation of algal organic matter: towards improved in-situ fluorometer development
This document is posted to help you gain knowledge. Please leave a comment to let me know what you think about it! Share it to your friends and learn new things together.
Transcript
Fluorescence spectroscopic characterisation of algal organic
matter: towards improved in-situ fluorometer development
Sara I. Khan 1, Arash Zamyadi 1, 2, 3, Narasinga Rao Hanumanth Rao 1, Xiang Li1, 2, Richard M.
Stuetz2, Rita K. Henderson 1, *
1 The bioMASS Lab, School of Chemical Engineering, The University of New South Wales, Sydney,
New South Wales, 2052, Australia
2 UNSW Water Research Centre, School of Civil and Environmental Engineering, The University of
New South Wales, Sydney, New South Wales, 2052, Australia
3 Civil, Mineral and Mining Engineering Department, École Polytechnique de Montreal, Montreal,
Figure S1: AOM composition of (i) DC (ii) CR (iii) CV (iv) MA555) and (v) MA564 at different growth phases
Bio
poly
mer
s
Bui
ldin
g bl
ocks
LMW
Aci
ds a
nd H
S
LMW Neutrals
Nitrate
Ammonium
Byp
ass
0 10 20 30 40 50 60 70 80 90 1000
2
4
6
8
10
12
Retention Time in Minutes
Rel
. Sig
nal R
espo
nse
-- OCD × 0.5
Exponential phase
Stationary phase
Decline phase
(A)
Bio
poly
mer
s
Bui
ldin
g B
lock
s
LMW Neutrals
Nitrate
Ammonium
Byp
ass
0 10 20 30 40 50 60 70 80 90 1000
2
4
6
8
Retention Time in Minutes
Rel
. Sig
nal R
espo
nse
-- OCD × 1-- UVD × 1-- OND × 0.5
Exponential phase
Stationary phase
Decline phase
(B)
Biop
olym
ers
Build
ing
Bloc
ks
LMW
Aci
ds a
nd H
S
LMW Neutrals
Nitrate
Ammonium
Bypa
ss
0 20 40 60 80 1000
2
4
6
8
10
12
Retention Time in Minutes
Rel
. Sig
nal R
espo
nse
-- OCD × 0.5-- UVD × 1
Exponential phase
Stationary
Decline phase
(C)
Bio
poly
mer
s
Bui
ldin
g B
lock
s
LMW
Aci
ds a
nd H
S
LMW Neutrals
Nitrate
Ammonium
Byp
ass
0 20 40 60 80 1000
2
4
6
8
10
12
Retention Time in Minutes
Rel
. Sig
nal R
espo
nse
-- OCD × 0.5-- UVD × 1
Exponential Phase
Stationary phase
Decline
(D)
Figure S2: LC-OCD chromatograms of AOM from (A) MA555 (B) CR (C) CV and (D) DC at different growth phase
Figure S3: Contour plots of the six AOM components identified from the complete F-EEMs dataset
Figure S4: Normalized fluorescence intensity of observed AOM components along the growth phase of species
DC CR CV MA555 MA554 MA564 MA566
Exponential Stationary0
1
2
3
4
5C1 C2 C4 C6 C3
Fmax
Exponential Stationary0
0.1
0.2
0.3
0.4
0.5
C1 C2 C4 C6C3 C5
Fmax
/DO
CFigure S5: Fmax and Fmax/DOC for DC for observed AOM components
Exponential Stationary0
0.4
0.8
1.2
1.6C1 C2 C4 C6
Fmax
Exponential Stationary0
0.1
0.2
0.3
0.4
0.5C1 C2 C4 C6 C3C5
Fmax
/DO
C
Figure S6: Fmax and Fmax/DOC for CR for observed AOM components
Exponential Stationary0
20
40
60
80
100
120
C1 C2 C4 C6C3 C5
Fmax
Exponential Stationary0
2
4
6
8
10
C1 C2 C4 C6C3 C5
Fmax
/DO
CFigure S7: Fmax and Fmax/DOC for CV for observed AOM components
Exponential Stationary0
5
10
15
20
25
C1 C2 C4 C6C3 C5
Fmax
Exponential Stationary
0
0.2
0.4
0.6
0.8
1
C1 C2 C4 C6 C3C5
Fmax
/DO
C
Figure S8: Fmax and Fmax/DOC for MA555 for observed AOM components
Exponential Stationary0
0.4
0.8
1.2
1.6
2C1 C2 C4 C6
Fmax
0.143695361
0.078764963
Exponential Stationary0
0.1
0.2
0.3
0.4
0.5
C1 C2 C4 C6 C3C5
Fmax
/DO
C
Figure S9: Fmax and Fmax/DOC for MA554 for observed AOM components
Exponential Stationary0
0.4
0.8
1.2
1.6
2
C1 C2 C4 C6C3 C5
Fmax
Exponential Stationary
0
0.1
0.2
0.3
0.4
0.5
C1 C2 C4 C6C3 C5
Fmax
/DO
C
Figure S10: Fmax and Fmax/DOC for MA566 for observed AOM components
Exponential Stationary0
0.4
0.8
1.2
1.6C1 C2 C4 C6 C3C5
Fmax
Exponential Stationary
0
0.1
0.2
0.3
0.4
0.5
C1 C2 C4 C6 C3C5
Fmax
/DO
C
Figure S11: Fmax and Fmax/DOC for MA564 for observed AOM components
C1 C2 C40
1
2
3
4
5
6
7
DC CR CV MA555 MA554 MA564 MA566Fm
ax (R
U)
(A)
bB bA bA
aB
abBabA
aA
bA
cA cA
aA
cB cB cB cB cAbcA cC
abA
cAB
aA
C3 C5 C60
2
4
6
8
10
12
Fmax
(RU
)
(B)
cA
dA
aA
bA
dA dAdA
bB bA abCaB abA abA abA
bB bA
aB
bB bA bA bA
Figure S12 (A & B): Interaction averages of observed fluorophores during exponential phase
For statistical comparison of means, same upper-case letters indicate no significant effect of changes in AOM fluorophores for a same species, and same lower-case letters indicate no significant difference among species for a particular AOM component
C1 C2 C40
2
4
6
8
10
DC CR CV MA555 MA554 MA564 MA566Fa
mx
(RU
)
abB
bA
abBabB
abAabA aA
bA
cA
bA
aA
cB cA cB bB bA
aA
bB
bAbA
bA
(A)
C3 C5 C60
20
40
60
80
100
120
DC CR CV MA555 MA554 MA564 MA566
Fmax
(RU
)
cA cA
aA
bA
aA aAaB
aB aB aB
(B)
Figure S13 (A & B): Interaction averages of observed AOM components during stationary phase
For statistical comparison of means, same upper-case letters indicate no significant effect of changes in AOM fluorophores for a same species, and same lower-case letters indicate no significant difference among species for a particular AOM component
C1 C2 C3 C4 C5 C60
5
10
15
20
25Exponential
StationaryFm
ax (R
U)
Figure S14: Single factor averages of observed AOM components (C1-C6) across exponential and stationary phase