Oxidative phosphorylation • Chemiosmotic coupling hypothesis • Proton motive force • Production of ATP • NADH from Glycolysis • Total ATP • Transport of ATP • Rates of Respiration
Oxidative phosphorylation• Chemiosmotic
coupling hypothesis• Proton motive force• Production of ATP• NADH from
Glycolysis• Total ATP • Transport of ATP • Rates of Respiration
Chemiosmotic coupling hypothesis
• Peter Mitchell • Movement of H+
across inner membrane produces gradient
Proton motive force
• Two components – membrane potential – pH gradient
• Calculate gradient using equation – pH gradient 1.4 units
in liver and E. coli• 12 H+ pumped out/
pair of electrons
F1 stalk, matrix of mitochondria
• Α3, β3, γ,δ,ε subunits: β interacts with γ.• T(tight) = binds ATP• L (loose) = ADP & Pi• O (open) = bound or release nucleotide• Rotation of γ subunit due to movement of protons
through Fo
Fo in membrane
• Fo hydrophobic, proton channel• a subunit and c rings (10-14 rings)• H+ enters cytosolic channel neutralize aspartic acid• c ring rotates• H+ leaves matrix channel, aspartic acid charged• repeat
Production of ATP
• 2.5 ATP/NADH • 1.5 ATP/FADH • How much ATP? • Pyruvate to Acetyl
CoA • TCA cycle
Rates of Respiration
• O2 consumption versus time
• Addition of ADP needed
• calculate P/O ratio of different compounds