Overexpression of HER2 (erbB2) in human breast epithelial ... › content › early › 2004 › 03 › 24 › jbc.M400081200.full.pdf · 1 Overexpression of HER2 (erbB2) in human

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Overexpression of HER2 (erbB2) in human breast epithelial cells unmasks

TGFβ-induced cell motility

Yukiko Ueda1, Shizhen Wang2, Nancy Dumont2, Jae Youn Yi1, Yasuhiro Koh1, and Carlos L.

Arteaga1,2,3,4

Departments of Medicine1 and Cancer Biology2, Vanderbilt University School of Medicine; and

Breast Cancer Research Program, Vanderbilt-Ingram Comprehensive Cancer Center3, Nashville,

TN 37232

4 To whom correspondence should be addressed at:

Division of Oncology

Vanderbilt University School of Medicine

2220 Pierce Avenue, 777 PRB

Nashville, TN 37232-6307

Tel. 615-936-3524

Fax 615-936-1790

[email protected]

Running Title:

TGFβ-induced mammary epithelial cell motility

by guest on August 3, 2020

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Abstract

We have examined if overexpression of the human epidermal growth factor receptor 2 (HER2)

modifies the anti-proliferative effect of TGFβ against MCF-10A human mammary epithelial

cells. Exogenous TGFβ inhibited cell proliferation and induced Smad-dependent transcriptional

reporter activity in both MCF-10A/HER2 and MCF-10A/vector control cells. Ligand-induced

reporter activity was 7-fold higher in HER2-overexpressing cells. In wound closure and

transwell assays, TGFβ induced motility of HER2-transduced but not control cells. The HER2-

blocking antibody trastuzumab (Herceptin) prevented TGFβ-induced cell motility. Expression of

a constitutively active TGFβ type I receptor (ALK5T204D) induced motility of MCF-10A/HER2

but not MCF-10A/vector cells. TGFβ-induced motility was blocked by coincubation with either

the phosphatidylinositol-3 kinase (PI3K) inhibitor LY294002, the mitogen-activated protein

kinase (MAPK) inhibitor U0126, the p38 MAPK inhibitor SB202190, and an integrin β1

blocking antibody. Rac1 activity was higher in HER2-overexpressing cells, where both Rac1 and

Pak1 proteins were constitutively associated with HER2. Both exogenous TGFβ and

transduction with constitutively active ALK5 enhanced this association. TGFβ induced actin

stress fibers as well as lamellopodia within the leading edge of wounds. Herceptin blocked basal

and TGFβ-stimulated Rac1 activity but did not repress TGFβ-stimulated transcriptional reporter

activity. These data suggest that 1) overexpression of HER2 in nontumorigenic mammary

epithelial is permissive for the ability of TGFβ to induce cell motility and Rac1 activity, and 2)

HER2 and TGFβ signaling cooperate in the induction of cellular events associated with tumor

progression.


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Introduction

The transforming growth factor (TGF) βs are polypeptides that are part of the

superfamily of structurally-related ligands which include the TGFβs, activins, and bone

morphogenetic proteins (BMPs). TGFβ ligands can modulate cell proliferation, lineage

determination, functional differentiation, extracellular matrix production, cell motility, and

apoptosis (1). TGFβ was originally reported to induce anchorage-independent growth of mouse

fibroblasts (2). Later studies indicated that TGFβ is a potent inhibitor of epithelial cell

proliferation (3,4), suggesting a role in tumor suppression. Transgenic mice expressing active

TGFβ1S223/225 in the mammary gland exhibit delayed ductal morphogenesis, renewal capacity,

and functional differentiation (5-7). Inhibition of autocrine TGFβ signaling in the mouse

mammary gland by tissue-specific expression of a truncated dominant-negative TGFβ type II

receptor (TβRII) results in accelerated lobulo-alveolar development (8) and enhanced propensity

for the development of carcinogen-induced lung, mammary, and skin tumors (9,10) as well as

spontaneous invasive mammary carcinomas (11). Mice with targeted disruption of either the

Tgfb1 or the Smad3 genes develop colon adenomas and carcinomas (12,13). Mice heterozygous

for deletion of the Tgfb1 gene express 10-30% of the TGFβ1 protein and this partial loss is

sufficient to attenuate epithelial growth restraint and inhibit tumor suppression function, resulting

in enhanced carcinogen-induced liver and lung tumors (14). MMTV/TGFβ1 mice are resistant to

DMBA-induced mammary tumors and when cross-bred with mice expressing the EGF receptor

ligand TGFα in the mammary gland (MMTV/TGFα), the bigenic (TGFβ/TGFα) progeny fails to

develop TGFα-induced mammary hyperplasias and carcinomas while still exhibiting a

hypoplastic phenotype (15). Finally, mice overexpressing active TGFβ1 under the control of the


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K14 keratinocyte-specific promoter are relatively protected from skin tumors induced by

chemical carcinogens (16), further supporting the tumor suppressive role of TGFβ signaling.

Most cancer cells loose or attenuate TGFβ-mediated anti-proliferative effects either by

mutational inactivation of TGFβ receptors or their signal transducers or by less known

mechanisms [reviewed in (17-19)]. There is also abundant experimental evidence to support that

excess production and/or activation of TGFβ in tumors, including breast cancers, can foster

cancer progression by autocrine and paracrine mechanisms [reviewed in (20)]. These include

enhancement of tumor cell motility and survival, increase in tumor neo-angiogenesis and

extracellular matrix production, upregulation of peritumor metalloproteases, and inhibition of

host immune surveillance mechanisms (17,21). For example, overexpression of active TGFβ1 or

an activated type I TGFβ receptor (TβRI) in the mammary gland of transgenic mice results in

acceleration of metastases derived from neu-induced primary mammary tumors (22,23). Further,

in transgenic mice expressing the Polyomavirus middle T antigen (PyVmT) in mammary

epithelium, blockade of TGFβ with a soluble fusion protein consisting of the extracellular

domain of TβRII and the Fc domain of human IgG1 (sTβRII:Fc) results in increased apoptosis in

primary mammary tumors and a reduction in both circulating tumor cells and lung metastases

(24). Taken together, these data suggest that 1) TGFβ can suppress some but not all oncogenic

signals in epithelial cells, and 2) some oncogenes can engage TGFβ signaling and utilize it for

tumor maintenance and progression.

We have studied if the HER2 proto-oncogene product, the human homolog of erbB2 and

neu, modulates cellular responses to TGFβ in MCF-10A nontumorigenic human mammary

epithelial cells. The HER2 protein is a member of the erbB family of transmembrane receptor


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tyrosine kinases which also includes the EGF receptor (HER1, erbB1), HER3 (erbB3), and

HER4 (erbB4). Binding of ligands to EGFR, HER3, and HER4 induces the formation of

homodimeric and heterodimeric, kinase-active complexes to which HER2 is recruited as a

preferred partner (25). Even though HER2 is unable to interact directly with receptor ligands, it

can amplify signaling pathways activated by erbB co-receptors, which include PLCγ-1, Ras-Raf-

MEK-MAPK (ERK), PI3K-Akt-p70S6 kinase, Pak-JNNK-JNK, SAPKs, and STATs (26). Some

of these pathways such as ERK, p38 MAPK, PI3K, and JNK are also stimulated by TGFβ in

some cells (18,27), thus providing a biochemical basis for synergy between TGFβ and erbB

receptor signaling.

As indicated above, TGFβ can suppress TGFα-induced transformation in the mammary

gland (15). Furthermore, TGFβ can also suppress EGF-induced mitogenesis in epithelial cells

(28). These results imply that TGFβ is dominant over the EGF receptor. Because of the ability of

HER2 to signal with increased potency than the EGF receptor (26), we speculated that

overexpression of HER2 may prevent TGFβ-mediated growth inhibition and/or modulate other

cellular responses of non-transformed mammary epithelial cells to this ligand. In this report, the

proliferation of MCF-10A cells overexpressing stably transduced HER2 was still delayed by

exogenous TGFβ. However, MCF-10A/HER2 cells exhibited increased motility and Rac1

activity in response to TGFβ, suggesting that HER2 and TGFβ signals can cooperate in inducing

cellular events required for tumor progression.


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Experimental Procedures

Cell culture and reagents. MCF-10A cells were purchased from the American Type Culture

Collection (Manassas, VA) and maintained in 1:1 mixture of Dulbecco’s modified Eagle’s

medium (DMEM) and Ham’s F12 medium supplemented with horse serum, L-glutamine, human

recombinant EGF (Calbiochem, San Diego, CA), cholera toxin (Biomol, Plymouth Meeting,

PA), insulin (Calbiochem), and hydrocortisone (Sigma, St. Louis, MO). Experiments were

performed in serum-free DMEM/F12 medium supplemented with 0.2% BSA (fraction V). 293T

cells were maintained in DMEM/10% heat-inactivated fetal calf serum (FCS). All tissue culture

reagents were from Life Technologies, Inc. (Rockville, MD) unless otherwise specified. Human

recombinant TGFβ1 was from R&D Systems (Minneapolis, MN) and the humanized IgG1 HER2

antibody trastuzumab (Herceptin) was purchased from the Vanderbilt University Medical Center

Pharmacy. ZD1839 (Iressa) was kindly provided by Alan Wakeling (AstraZeneca

Pharmaceuticals, Macclesfield, UK). LY294002 and SB202190 were from Calbiochem, U-0126

from Promega (Madison, WI), and the integrin β1 blocking antibody was from Chemicon

(Temecula, CA).

Retroviral and adenoviral vectors. c-Myc-tagged HER2 cDNA (provided by Dr. James Staros,

Vanderbilt University) was excised using Xba-I/Hind-III enzymes, purified by agarose-gel

electrophoresis, and converted to blunt ends with Klenow DNA polymerase. The retroviral

expression vector pBMN-IRES-EGFP (29) (provided by Dr. Gary Nolan, Stanford University)

was digested with Sna-BI and dephosphorylated at the 5’-ends with alkaline phosphatase

(Promega). The blunt-ended vector and the HER2 cDNA were ligated and then replicated in

DH5α (Life Technologies, Inc.). To confirm that the HER2 insert had no truncations, the vector

was digested with Stu-I and also sequenced using the primer 5’-GACCTTACACAGTCCTGC-


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3’. Restriction enzymes, T4-DNA ligase, and DNA polymerases were all from New England

BioLabs (Beverly, MA). 293T retrovirus-packaging cells (2.5x106/60-mm dish; ATCC) were

transfected with 5 µg pBMN-HER2-IRES-EGFP or 5 µg pBMN-IRES-EGFP (control) for 24 h

using FUGENE 6 (Roche Molecular Biochemicals, Indianapolis, IN). For each case, cells were

cotransfected with 3 µg pHCMV-G (30) and 3 µg pSV-Ψ-env-MLV (pSV-pol/gag) (31)

(provided by Dr. Jane Burns, University of California, San Diego). Virus-containing medium

was collected 48-72 h later and passed through a 45-µm filter. MCF-10A cells (105/60-mm dish)

were transduced with control or HER2-encoding retroviral vectors for 2 h. After 5 passages, cells

stably expressing EGFP were sorted by flow cytometry and expanded. EGFP expression was

monitored with an inverted fluorescent microscope and maintained at 100% throughout all

experiments.

The HA-tagged ALK5T204D adenoviral construct was provided by Dr. Kohei Miyazono

(Japanese Foundation for Cancer Research, Tokyo, Japan). Stock of recombinant viruses were

replicated in 293 cells and tittered using the Adenovirus Expression Vector Kit (Takara

Biomedicals, Tokyo). MCF-10A cells were infected with ALK5T204D or a control β-galactosidase

adenovirus at a multiplicity of infection (MOI) of 10 plaque-forming units (PFU) per cell for 1 h,

unless otherwise specified. After 48 h, β-gal expression was assessed using β-galatosidase

Enzyme System (Promega). Expression of HA-tagged mutant ALK5 was confirmed by HA and

TβRI immunoblot analyses.

Immunoblot analysis and immunoprecipitation. Cells were washed twice with ice-cold PBS

and lysed with a buffer containing 50 mM Tris (pH 7.4), 150 NaCl, 1% Nonidet P-40, 0.5% Na

deoxycholate, 1 mM EDTA, 20 mM NaF, 1 mM Na3VO4, 1 mM PMSF, and 2 µg/ml of each

aprotinin, pepstatin, and leupeptin. Protein content was quantitated using the BCA protein assay


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reagent (Pierce). Protein extracts were separated by SDS-PAGE, transferred to nitrocellulose

membrane, and subjected to immunoblot analysis as described previously (32). Immunoreactive

bands were visualized by chemiluminescence (Roche Molecular Biochemicals).

Immunoprecipitations were performed as described previously (32) after preclearing cell lysates

with protein A-sepharose (Sigma) for 2 h at 4°C. The antibodies utilized were: HER2 (erbB2

Ab1) and HER3 (erbB3 C-17; NeoMarkers, Union City, CA); c-Myc (9E10; Roche);

Hemagglutinin (HA), TβRI (V22), and Pak1 (Santa Cruz Biotechnology, Santa Cruz, CA); Rac1

(Transduction Laboratories, Lexington, KY); P-Tyr monoclonal (Upstate Biotechnology, Lake

Placid, NY).

Cell cycle analysis. Cells were harvested by trypsinization and labeled with 50 µg/ml propidium

iodide (Sigma) containing 125 U/ml protease-free RNAse (Calbiochem). A total of 10,000

stained nuclei were analyzed in a FACS/Calibur Flow Cytometer (Beckton Dickinson,

Mansfield, MA) as described previously (33).

Cell motility assays and BrdU incorporation. Cell motility was assessed in wound closure

assays as described previously (32). For wound closure experiments, cells were allowed to reach

confluence in serum-containing complete growth medium and then incubated for 16 h in serum-

free medium (SFM) prior to the addition of TGFβ or other inhibitors. Wound closure was

monitored by microscopy at various times. In some cases, wounded monolayers were incubated

with BrdU (Zymed, San Francisco, CA) for 3 h and then stained with a biotinylated BrdU

antibody according to the BrdU Staining Kit protocol (Zymed). Transwell migration was

performed utilizing 5-µm pore, polycarbonate filters (Corning Costar Corp., Cambridge, MA) as

described (32). Cells migrated to the underside of transwell filters were fixed, stained with Diff-


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Quick (Düdingen, Switzerland), and counted by bright field microscopy at 200x in five random

fields.

Transcription reporter assays. Cells were seeded in 24-well plates and transfected with 3 µg

per 35-mm dish of the Smad-dependent reporter contruct p(CAGA)12-luciferase (provided by J.-

M. Gauthier, Laboratoire Glaxo Wellcome, Les Ulis Cedex, France) along with 0.02 µg per well

of pCMV-Renilla using FUGENE 6 according to the manufacturer’s protocol. Firefly and

Renilla reniformis luciferase activities were measured using the Dual Luciferase Assay System

(Promega) and the data were normalized utilizing the ratio of Firefly to R. reniformis luciferase

as described previously (32).

Immunofluorescent microscopy. Cells were treated with TGFβ1 on glass coverslips and then

fixed and permeabilized as described previously (34) followed by incubation with phalloidin-

Texas Red (2 U/ml in PBS for 30 min; Molecular Probes). Fluorescent images of coverslips

mounted in AquaPolyMount (Polysciences) were captured using a Princeton Instruments cooled

CCD digital camera from a Zeiss Axiophot upright microscope.

Rac1 activity assay. The pGEX plasmid containing GST-PBD (glutathione S-transferase fused

with the Pak family binding domain for Cdc42/Rac1) (35) was a gift from R. Cerione (Cornell

University, New York, NY). GST-PBD was expressed in E. coli and immobilized by

glutathione-sepharose 4B beads following the GST Gene Fusion System protocol (Pharmacia).

Fifty µg of GST-PBD were incubated with 800 µg of protein from precleared cell lysates for 3 h

at 4°C. GST-PBD was affinity-precipitated using glutathione-sepharose 4B beads (Pharmacia)

and the adsorbed proteins were eluted from the beads by suspending them in Laemmli sample

buffer followed by SDS-PAGE and Rac1 immunoblot analysis as described previously (34).


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cDNA array and Northern analysis. Cells were incubated for 16 h in SFM and then treated

with TGFβ1 for 24 h. Total RNAs were isolated with Trizol Reagent (Life Technologies),

digested with RNase-free DNase (0.1 unit/1 µg RNA; Promega), and purified using a silica gel

column (RNeasy Kit, Qiagen). Purified RNAs (50 µg) were reverse transcribed to cDNAs and

these were labeled with Cy3-conjugated dCTP (MCF-10A/HER2) or Cy5-conjugated dCTP

(MCF-10A/vector). The procedures for reverse transcription, RNA template digestion, and

cDNA purification and hybridization to a human 5K cDNA array were performed following

protocols listed in www.array.vanderbilt.edu. Cy3- and Cy5-labeled cDNAs were scanned at 532

and 635 nm, respectively, in GenePix Pro (Axon Instruments, Inc., Foster City, CA). DNA

polymerase, primers, and fluorescent dye-conjugated nucleotides were from Life Technologies

unless otherwise specified. For Northern analysis, RNAs were resolved by agarose gel

electrophoresis, transferred onto nylon membranes (Schleicher & Shell, Keene, NH), and

hybridized with cDNA probes using Express Hybridization Solution (Clontech, Palo Alto, CA)

following the manufacturer’s protocol. The cDNA probes were made from 3’-ESTs excised from

cloning vectors: 1) TβRII 3’-EST (accession #: AA487034, restriction enzymes: EcoR-I/Xho-1);

2) Metallothionein I-H (H77597, EcoR-I/Pac-1). The excised 3’-ESTs were isolated by agarose

gel electrophoresis, labeled with [α-32P]dCTP (NEN Life Science Products) using the Random

Primer Labeling System (Life Technologies), and purified with a silica gel column (Qiagen).


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Results

TGFβ induces motility of MCF-10A/HER2 cells.---------- We have examined the effects

of HER2 overexpression on the response of human mammary epithelial cells to TGFβ. MCF-

10A nontumorigenic mammary epithelial cells were stably transduced with a retroviral vector

encoding Myc-tagged HER2 (Fig. 1A). By western analysis with HER2 and c-Myc antibodies,

proto-oncogene expression was confirmed in the transduced cells. Both HER3 and a 185-kDa

phospotyrosine band were identified in HER2 immunoprecipitates from the HER2 transfected

cells indicating that HER2 was constititutively phosphorylated and associated with HER3

(erbB3) in the absence of exogenous ligands (Fig. 1B). Treatment with recombinant TGFβ (for

24 h) increased the fraction of cells in G1 phase of the cell cycle in MCF-10A/HER2 (34→47%)

and control cells (32→49%) with a simultaneous reduction in the S-phase fraction and a 50%

reduction in cell number after 6 days (Figs. 1C,D).

We next examined the effect of TGFβ on the motility of HER2-overexpressing cells in

wound closure and transwell motility assays. After 18 h, MCF-10A/HER2 cells migrated into the

wounded area but did not close the wound whereas in MCF-10A/vector cells the wound

remained completely open. Similar results were observed in cells plated in transwells (Fig. 2A,B,

first column). Addition of TGFβ resulted in complete wound closure by MCF-10A/HER2 cells

but had no effect on motility of control cells (Fig. 2A,B, second column). TGFβ-induced motility

was blocked by addition of a saturating concentration of the HER2 blocking antibody Herceptin

(Fig. 2A,B), thus implying that the effect of TGFβ was HER2 specific. The EGF receptor

tyrosine kinase inhibitor ZD1839 (Iressa), which has been shown to block phosphorylation of

HER2 (36) also inhibited TGFβ-induced wound closure completely (data not shown).


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Expression of ALK5T204D enhances motility in MCF-10A/HER2 cells.--------- To

determine if the motility effect was specific to activated TGFβ receptors, we induced TGFβ

signaling in both cell types by expressing an HA-tagged adenovirus encoding a constitutively

active mutant of TβRI (ALK5T204D). Mutation of threonine 204 in ALK5 to aspartic acid leads to

constitutive activation of the type I receptor serine/threonine kinase allowing it to signal in the

absence of added ligand or TβRII (37). Cells infected with a β-galactosidase adenovirus at the

same MOI were used as controls. The efficiency of transduction was >90% as evaluated by in

situ staining for β-galactosidase activity 48 h after infection (data not shown). Expression of

ALK5T204D was confirmed by HA immunoblot analysis (Fig. 3A). The β-gal transduced MCF-

10A/HER2 cells exhibited slightly higher basal motility than the non-motile MCF-10A/vector

cells. However, ALK5T204D induced wound closure and transwell migration in HER2-

overexpressing but not in control cells (Fig. 3B,C).

TGFβ-induced motility of HER2-overexpressing MCF-10A cells requires multiple

signaling pathways. ------------ TGFβ-induced cell motility in HER2 cells was blocked by the

addition of mitomycin C, a compound that inhibits DNA synthesis (data not shown). Therefore,

we examined if TGFβ stimulated DNA synthesis in the wounded monolayers as measured by

BrdU incorporation. Interestingly, addition of TGFβ clearly stimulated BrdU incorporation in

MCF-10A/HER2 but not MCF-10A/vector cells but this was limited to cells in the edge of the

wound (Fig. 4A). Since TGFβ can activate non-Smad signaling pathways or induce molecules

associated with cell motility (32,34), we examined for evidence of activated (phosphorylated)

Akt, ERK, p38 MAPK as well as increased integrin β1 by immunocytochemistry focusing on

cells in the leading edge of the wound. Using commercially available (phospho-specific)

antibodies, we were unable to detect the above mentioned molecules. However, TGFβ-induced


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wound closure was completely blocked in the presence of the PI3K inhibitor LY294002, the

MEK1/2 inhibitor U0126, the p38 MAPK inhibitor SB202190, and the CD29 integrin β1

blocking antibody (Fig. 4B).

TGFβ induces actin reorganization and lamellopodia in MCF-10A/HER2 cells. ----------

We next investigated if reorganization of the actin cytoskeleton, which is required for cell

migration, was being induced by exogenous TGFβ. Wounded monolayers of HER2-

overexpressing and vector control cells were stimulated with TGFβ. Within 15 min, actin stress

fibers, as measured by phalloidin-Texas Red, formed along the largest axis in MCF-10A/HER2

cells within the leading edge of the wound. In addition, the TGFβ-stimulated advancing cells

exhibited membrane ruffling and the formation of lamellipodia. These changes were not seen in

MCF-10A/vector cells. (Fig. 5A). These changes suggested activation of the Rac1 and Cdc42

GTPases (38). Rac1 and Cdc42 activity was measured by precipitating active GTP-bound forms

of these proteins using a GST fusion protein containing the p21-activated kinase (Pak) binding

domain (GST-PBD). The Pak family of 62-68 kDa serine/threonine kinases are the best

characterized effector proteins of Rac1 and Cdc42 and have been shown to bind preferentially to

the GTP-bound forms of these proteins (39). Total Rac1 levels were unchanged in HER2-

transduced compared to control cells (Fig. 5B, top). However, higher levels of Rac1 were present

in the GST-PBD eluated of MCF-10A/HER2 compared to control cells, suggesting higher levels

of Rac1 activity. Preincubation with Herceptin inhibited both basal and TGFβ-stimulated Rac1

activity in the HER2-transduced but not control cells (Fig. 5B, bottom). Cdc42 was undetectable

by immunoblot analysis in the GST-PBD eluates (data not shown).

Since constitutively active TβRI (ALK5T204D) mimicked the ligand-induced effect on cell

motility (Fig. 3), we next determined whether active ALK5 interacted with Rac and Pak.


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Transduction of mutant ALK5 resulted in Smad2 phosphorylation but did not alter Rac1 and

Pak1 protein levels in MCF-10A/HER2 cells (Fig. 5C, left). Interestingly, HER2 was detected by

immunoblot analysis in Rac1 and Pak1 precipitates from MCF-10A/HER2 cell lysates under

basal conditions. This association was increased by both the addition of TGFβ (within 15 min) or

transduction with ALK5T204D but not with the β-galactosidase control adenovirus. HA

immunoblots of Rac1 pulldowns revealed the presence of ALK5T204D (Fig. 5C, right), implying

that in HER2-overexpressing cells, activation of TGFβ results in the association of HER2, Rac,

Pak, and the type I TGFβ receptor.

TGFβ enhances Smad-dependent transcription and differential gene expression in MCF-

10A/HER2 cells. ------------ The ability of TGFβ to inhibit proliferation of MCF-10A/HER2

monolayers (Fig. 1C, D) implied that Smad function was retained in cells overexpressing HER2.

On the other hand, the different effect on motility as a function of HER2 overexpression

suggested the possibility of differential ligand-induced gene expression. To test TGFβ-induced

transcription, a reporter construct containing 12 Smad binding elements repeated in tandem,

p(CAGA)12-luciferase, was transiently transfected into MCF-10A/HER2 and MCF-10A/vector

cells, along with pCMV-Renilla. Normalized luciferase activity indicated that TGFβ1 induced

Smad-dependent transcription in both cell types but this induction was 7-fold higher in MCF-

10A/HER2 cells. Herceptin minimally reduced TGFβ-induced reporter activity in MCF-

10A/HER2 but not in vector control cells, suggesting that its ability to block ligand-induced

motility was independent of an inhibitory effect on gene transcription (Fig. 6A).

Because of the difference in magnitude of TGFβ-induced reporter activity between both

cell types, we next examined differential ligand-induced gene expression (at 24 h) in both cells


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by cDNA array analysis. The raw data for the differentially expressed genes in TGFβ-treated

cells is available in the Supplemental Table. There were 161 genes upregulated and 215 genes

downregulated at least 3-fold in TGFβ-treated MCF-10A/HER2 relative to TGFβ-treated MCF-

10A/vector cells. Fig. 6B contains 12 genes that were upregulated and 10 genes that were

downregulated, all with previously reported functions, in HER2-overexpressing cells relative to

vector controls. Among this list, the type II TGFβ receptor (TβRII) and the metallothionein 1H

genes were upregulated (3.5- and 8.1-fold, respectively) by added ligand in HER2 cells relative

to control cells. Upregulation of these two RNAs was confirmed by Northern analysis, which

indicated differences in steady state levels of these transcripts in both cell types (Fig. 6C)

consistent with the magnitude of difference reported in the cDNA array analysis.


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Discussion

We have examined if the HER2 proto-oncogene is dominant over TGFβ and whether it

can synergize with TGFβ on cellular responses associated with epithelial transformation.

Overexpression of HER2 in MCF-10A nontumor mammary cells did not abrogate TGFβ-

induced anti-mitogenic effects. However, it was clearly permissive for TGFβ-induced motility.

This motility was blocked by preincubation with Herceptin, a humanized IgG1 that binds to the

ectodomain domain of the HER2 receptor and blocks its function (40), suggesting that the effect

of TGFβ was HER2 specific. Similar to exogenous TGFβ, expression of activated TβRI without

added ligand also induced wound closure and transwell migration in HER2-overexpressing cells

but not in control cells with undetectable HER2 protein, further indicating that high levels of

HER2 are required for TGFβ-stimulated cell migration. The retention of the antiproliferative

effect of TGFβ with the simultaneous induction of cell motility, which is associated with a more

transformed phenotype, is consistent with a recent report of bi-transgenic tumors expressing neu,

the rat homolog of HER2, and active TGFβ1. Mammary tumors arising in MMTV/neu x

TGFβ1S223/225 bi-transgenic mice were smaller and less proliferative than cancers occurring in

MMTV/neu mice. However, the bigenic tumor cells were more motile, locally invasive, and

metastatic than tumors expressing neu alone (23). In addition, transgenic mice expressing

activated forms of neu and activated ALK5 in the mammary gland exhibit longer mammary

tumor latency compared to mice expressing MMTV/neu alone. However, the bigenic mice show

a higher frequency of extravascular lung metastases (22).

Taken together, these data imply that the antimitogenic and prometastatic effects of

TGFβ can exist simultaneously. Similar to blockade of HER2 with Herceptin, blockade of PI3K,


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ERK, p38 MAPK, and integrin β1 with specific inhibitors completely prevented TGFβ-

stimulated migration of MCF-10A/HER2 cells in wound closure assay (Fig.4B). These

molecules have all been shown to be associated with epithelial to mesenchymal transition (EMT)

and cell migration stimulated by TGFβ (32,34,41-44). Whether these signaling/adhesion

molecules are specific to the synergistic effects of TGFβ and HER2 on cell motility or whether

these results suggest the requirement of these pathways for any type of cell movement not

necessarily associated with TGFβ and HER2 require further investigation.

One obvious rapid response to exogenous TGFβ in HER2-overexpressing cells, but not

observed in control cells, was the formation of actin stress fibers as well as membrane ruffling

and lamellipodia. This result suggested the involvement of the Rac1 and Cdc42 GTPases. Indeed

activation of HER2 with heregulin has been shown to reorganize the actin cytoskeleton and

activate Pak1, a direct target of Rac1 (45), thus enhancing cell migration. Consistent with this

previous report, HER2 was constitutively phosphorylated in MCF-10A/HER2 cells and these

cells exhibited constitutive association of HER2 with Pak1 and Rac1 and higher basal Rac

activity than control cells with low HER2 levels. Both the addition of TGFβ and transduction

with constitutively active ALK5T204D markedly enhanced this association. Interestingly, ALK5

was detectable in Rac1 pulldowns, and this pulldowns also contained HER2 and Pak1 (Fig. 5C).

Whether the endogenous type I TGFβ receptor is part of such ligand-activated complex in non-

transduced cells requires further investigation. Data consistent with this result were reported

recently: Rac1 activity in situ was higher in transgenic tumors expressing neu and active TGFβ1

compared to tumors expressing neu alone (23). In NMuMG mammary epithelial cells, TGFβ

activates Rac1 which, in turn, activates p38 MAPK, a serine-threonine kinase involved in TGFβ-

mediated EMT and cell motility (34,43,46).


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In the report herein, Herceptin inhibited basal and TGFβ-stimulated Rac1 activity in

MCF-10A/HER2 cells, suggesting that the latter was required for ligand-induced cell motility.

Based on these data, we speculate that activated HER2 and TGFβ signaling converge at Rac1,

thus providing a threshold of sustained activation that leads to an enhanced migratory phenotype.

Rac1 has also been implicated in integrin-specific control of cyclin D1 expression and cell cycle

entry (47). The possible association between Rac1 activity and the enhanced DNA synthesis

observed in TGFβ-stimulated cells at the edge of closing wounds (Fig. 4A) remains to be

determined. Our data do not necessarily conflict with other possible mechanisms by which TGFβ

and HER2 can cooperate in the induction of cell migration and invasion. For example, MCF-10A

cells stably expressing activated human erbB2 (HER2) and TGFβ but not erbB2 alone secrete

autocrine factors that were sufficient to induce cell migration (48).

Using a heterologous promoter containing 12 Smad-binding elements as a reporter of

TGFβ-induced gene transcription, we found that HER2-overexpressing cells exhibited

significantly higher ligand-induced reporter activity. This result suggested differences in ligand-

induced gene expression between both cell types which were investigated by cDNA array

analysis. A list of differentially-upregulated or -downregulated genes in MCF-10A/HER2 vs.

MCF-10A/vector cells is included in Fig. 6B. Some of these are worth highlighting. For

example, steady-state levels of TβRII and Metallothionein 1H mRNAs were upregulated

approximately 3- and 8-fold, respectively, in ligand-stimulated HER2 compared to control cells.

Interestingly, studies in fibroblasts have shown upregulation of TGFβ binding in response to

injury (49). Metallothioneins are cysteine-rich, low-MW proteins that have been associated with

more virulent phenotypes of breast cancer (50,51). Three genes induced by TGFβ in HER2-

overexpressing cells have been associated with metastatic behavior of cancer cells: laminin (52),


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S110 calcium binding protein A2 (53), and metastasis-associated factor (mta1) (54). Vimentin, a

marker of fibroblasts or epithelial cells that have undergone EMT, and serum and glucocorticoid-

regulated kinase, a serine-threonine kinase which has been shown to protect mammary cells from

apoptosis (55,56), were also upregulated. Contrary to vimentin, keratin 8, a marker of epithelial

cells reported to be reduced in breast cancers relative to benign breast tissues (57), was

preferentially reduced by added ligand in MCF-10A/HER2 cells. The quiescin Q6 gene, whose

expression is induced as fibroblasts leave the cell cycle (58), was also downregulated in HER2

cells. Interestingly, the IL-1 receptor antagonist (IL-1 ra) gene was predominantly downregulated

in HER2 cells. IL-1 ra levels correlate directly with presence of estrogen receptors (ER) in breast

cancers. Since IL-1, which is induced by TGFβ in wounded keratinocytes (59), is also associated

with ER-negative, poor prognosis cancers (60), the downregulation of IL-1 ra might be

permissive for IL-1 actions.

In summary, overexpression of the HER2 tyrosine kinase in mammary epithelial cells

alters cellular responses to TGFβ. While this overexpression does not change the overall anti-

mitogenic effect of TGFβ, it is permissive for TGFβ-induced motility and gene expression.

These data suggest that HER2 and TGFβ signaling synergize in the transformation of mammary

epithelial cells. If operative in human breast cancers, such cooperation would provide a basis for

the combined use of HER2 and TGFβ inhibitors in patients with breast carcinoma.


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References

1. Massague, J. (1998) Annu Rev Biochem 67, 753-7912. Moses, H. L., Branum, E. L., Proper, J. A., and Robinson, R. A. (1981) Cancer Res 41,

2842-2848.3. Roberts, A. B., Anzano, M. A., Wakefield, L. M., Roche, N. S., Stern, D. F., and Sporn,

M. B. (1985) Proc Natl Acad Sci U S A 82, 119-123.4. Tucker, R. F., Shipley, G. D., Moses, H. L., and Holley, R. W. (1984) Science 226, 705-

707.5. Pierce, D. F., Jr., Johnson, M. D., Matsui, Y., Robinson, S. D., Gold, L. I., Purchio, A. F.,

Daniel, C. W., Hogan, B. L., and Moses, H. L. (1993) Genes Dev 7, 2308-2317.6. Kordon, E. C., McKnight, R. A., Jhappan, C., Hennighausen, L., Merlino, G., and Smith,

G. H. (1995) Dev Biol 168, 47-61.7. Jhappan, C., Geiser, A. G., Kordon, E. C., Bagheri, D., Hennighausen, L., Roberts, A. B.,

Smith, G. H., and Merlino, G. (1993) Embo J 12, 1835-18458. Gorska, A. E., Joseph, H., Derynck, R., Moses, H. L., and Serra, R. (1998) Cell Growth

Differ 9, 229-238.9. Bottinger, E. P., Jakubczak, J. L., Haines, D. C., Bagnall, K., and Wakefield, L. M.

(1997) Cancer Res 57, 5564-5570.10. Go, C., Li, P., and Wang, X. J. (1999) Cancer Res 59, 2861-2868.11. Gorska, A. E., Jensen, R. A., Shyr, Y., Aakre, M. E., Bhowmick, N. A., and Moses, H. L.

(2003) Am J Pathol 163, 1539-154912. Engle, S. J., Hoying, J. B., Boivin, G. P., Ormsby, I., Gartside, P. S., and Doetschman, T.

(1999) Cancer Res 59, 3379-3386.13. Zhu, Y., Richardson, J. A., Parada, L. F., and Graff, J. M. (1998) Cell 94, 703-714.14. Tang, B., Bottinger, E. P., Jakowlew, S. B., Bagnall, K. M., Mariano, J., Anver, M. R.,

Letterio, J. J., and Wakefield, L. M. (1998) Nat Med 4, 802-807.15. Pierce, D. F., Jr., Gorska, A. E., Chytil, A., Meise, K. S., Page, D. L., Coffey, R. J., Jr.,

and Moses, H. L. (1995) Proc Natl Acad Sci U S A 92, 4254-4258.16. Cui, W., Fowlis, D. J., Bryson, S., Duffie, E., Ireland, H., Balmain, A., and Akhurst, R. J.

(1996) Cell 86, 531-542.17. Derynck, R., Akhurst, R. J., and Balmain, A. (2001) Nat Genet 29, 117-129.18. Wakefield, L. M., and Roberts, A. B. (2002) Curr Opin Genet Dev 12, 22-29.19. Siegel, P. M., and Massague, J. (2003) Nat Rev Cancer 3, 807-82020. Dumont, N., and Arteaga, C. L. (2003) Cancer Cell 3, 531-53621. Dumont, N., and Arteaga, C. L. (2000) Breast Cancer Res 2, 125-13222. Siegel, P. M., Shu, W., Cardiff, R. D., Muller, W. J., and Massague, J. (2003) Proc Natl

Acad Sci U S A 100, 8430-843523. Muraoka, R. S., Koh, Y., Roebuck, L. R., Sanders, M. E., Brantley-Sieders, D., Gorska,

A. E., Moses, H. L., and Arteaga, C. L. (2003) Mol Cell Biol 23, 8691-870324. Muraoka, R. S., Dumont, N., Ritter, C. A., Dugger, T. C., Brantley, D. M., Chen, J.,

Easterly, E., Roebuck, L. R., Ryan, S., Gotwals, P. J., Koteliansky, V., and Arteaga, C. L.(2002) J Clin Invest 109, 1551-1559.

25. Olayioye, M. A., Neve, R. M., Lane, H. A., and Hynes, N. E. (2000) Embo J 19, 3159-3167.

26. Yarden, Y., and Sliwkowski, M. X. (2001) Nat Rev Mol Cell Biol 2, 127-137.


http://ww

w.jbc.org/

Dow

nloaded from

http://www.jbc.org/

21

27. Derynck, R., and Zhang, Y. E. (2003) Nature 425, 577-58428. Coffey, R. J., Jr., Sipes, N. J., Bascom, C. C., Graves-Deal, R., Pennington, C. Y.,

Weissman, B. E., and Moses, H. L. (1988) Cancer Res 48, 1596-1602.29. Grignani, F., Kinsella, T., Mencarelli, A., Valtieri, M., Riganelli, D., Lanfrancone, L.,

Peschle, C., Nolan, G. P., and Pelicci, P. G. (1998) Cancer Res 58, 14-1930. Yee, J. K., Miyanohara, A., LaPorte, P., Bouic, K., Burns, J. C., and Friedmann, T.

(1994) Proc Natl Acad Sci U S A 91, 9564-956831. Landau, N. R., and Littman, D. R. (1992) J Virol 66, 5110-511332. Dumont, N., Bakin, A. V., and Arteaga, C. L. (2003) J Biol Chem 278, 3275-3285.33. Shin, I., Bakin, A. V., Rodeck, U., Brunet, A., and Arteaga, C. L. (2001) Mol Biol Cell

12, 3328-3339.34. Bakin, A. V., Rinehart, C., Tomlinson, A. K., and Arteaga, C. L. (2002) J Cell Sci 115,

3193-3206.35. Bagrodia, S., Taylor, S. J., Creasy, C. L., Chernoff, J., and Cerione, R. A. (1995) J Biol

Chem 270, 22731-2273736. Moulder, S. L., Yakes, F. M., Muthuswamy, S. K., Bianco, R., Simpson, J. F., and

Arteaga, C. L. (2001) Cancer Res 61, 8887-889537. Wieser, R., Wrana, J. L., and Massague, J. (1995) Embo J 14, 2199-220838. Hall, A. (1998) Science 279, 509-514.39. Benard, V., Bohl, B. P., and Bokoch, G. M. (1999) J Biol Chem 274, 13198-1320440. Yakes, F. M., Chinratanalab, W., Ritter, C. A., King, W., Seelig, S., and Arteaga, C. L.

(2002) Cancer Res 62, 4132-414141. Janda, E., Lehmann, K., Killisch, I., Jechlinger, M., Herzig, M., Downward, J., Beug, H.,

and Grunert, S. (2002) J Cell Biol 156, 299-313.42. Bakin, A. V., Tomlinson, A. K., Bhowmick, N. A., Moses, H. L., and Arteaga, C. L.

(2000) J Biol Chem 275, 36803-36810.43. Bhowmick, N. A., Zent, R., Ghiassi, M., McDonnell, M., and Moses, H. L. (2001) J Biol

Chem 276, 46707-46713.44. Oft, M., Peli, J., Rudaz, C., Schwarz, H., Beug, H., and Reichmann, E. (1996) Genes Dev

10, 2462-2477.45. Adam, L., Vadlamudi, R., Kondapaka, S. B., Chernoff, J., Mendelsohn, J., and Kumar, R.

(1998) J Biol Chem 273, 28238-28246.46. Zhang, S., Han, J., Sells, M. A., Chernoff, J., Knaus, U. G., Ulevitch, R. J., and Bokoch,

G. M. (1995) J Biol Chem 270, 23934-23936.47. Mettouchi, A., Klein, S., Guo, W., Lopez-Lago, M., Lemichez, E., Westwick, J. K., and

Giancotti, F. G. (2001) Mol Cell 8, 115-12748. Seton-Rogers, S. E., Lu, Y., Hines, L. M., Koundinya, M., LaBaer, J., Muthuswamy, S.

K., and Brugge, J. S. (2004) Proc Natl Acad Sci U S A 101, 1257-126249. Song, Q. H., Singh, R. P., Richardson, T. P., Nugent, M. A., and Trinkaus-Randall, V.

(2000) J Cell Biochem 77, 186-19950. Friedline, J. A., Garrett, S. H., Somji, S., Todd, J. H., and Sens, D. A. (1998) Am J Pathol

152, 23-2751. Barnes, N. L., Ackland, M. L., and Cornish, E. J. (2000) Int J Biochem Cell Biol 32, 895-

90352. Chakrabarty, S., Liu, B. R., and Rajagopal, S. (2001) J Cell Physiol 186, 47-52


http://ww

w.jbc.org/

Dow

nloaded from

http://www.jbc.org/

22

53. Wang, G., Rudland, P. S., White, M. R., and Barraclough, R. (2000) J Biol Chem 275,11141-11146

54. Toh, Y., Pencil, S. D., and Nicolson, G. L. (1994) J Biol Chem 269, 22958-2296355. Leong, M. L., Maiyar, A. C., Kim, B., O'Keeffe, B. A., and Firestone, G. L. (2003) J Biol

Chem 278, 5871-588256. Mikosz, C. A., Brickley, D. R., Sharkey, M. S., Moran, T. W., and Conzen, S. D. (2001)

J Biol Chem 276, 16649-1665457. Czerwenka, K. F., Manavi, M., Hosmann, J., Jelincic, D., Pischinger, K. I., Battistutti, W.

B., Behnam, M., and Kubista, E. (2001) Cancer Detect Prev 25, 268-27958. Coppock, D. L., Cina-Poppe, D., and Gilleran, S. (1998) Genomics 54, 460-46859. Kang, K., Hammerberg, C., and Cooper, K. D. (1996) Exp Dermatol 5, 218-22660. Miller, L. J., Kurtzman, S. H., Anderson, K., Wang, Y., Stankus, M., Renna, M.,

Lindquist, R., Barrows, G., and Kreutzer, D. L. (2000) Cancer Invest 18, 293-302


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Acknowledgements

Supported by R01 CA62212 (C.L.A), Breast Cancer Specialized Program of Research

Excellence (SPORE) Grant P50 CA98131, and Vanderbilt-Ingram Comprehensive Cancer

Center Support Grant CA68485.


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Figure Legends

Figure 1. Overexpression of HER2 does not abrogate TGFβ-induced anti-proliferative effects.

A. Map of pBMN-IRES-EGFP retroviral vector. The HER2 cDNA containing the c-Myc tag

sequence was ligated at the Sna-B1 site. LTR, long terminal repeat; Psi(ψ), consensus sequence

for viral packaging; IRES, internal ribosomal entry sequence; EGFP, enhanced green fluorescent

protein. B. Parental MCF-10A, MCF-10A/vector, and MCF-10A/HER2 cell lysates were

subjected to immunoblot analysis with HER2, c-Myc, and actin antibodies. Where indicated,

lysates were precipitated with HER2 antibodies and the immune complexes were subjected to

immunoblot analysis with HER2, P-Tyr, and HER3 antibodies. C. Exponentially-growing cells

were treated or not with 1 ng/ml TGFβ1 for 24 h, labeled with propidium iodide, and then

analyzed by flow cytometry for cell cycle distribution. D. Cells were seeded on 12-well plates in

complete growth medium at a density of 3x104 cells/well. TGFβ1 (1 ng/ml) was added the next

day and cells were trypsinized and counted using a Coulter counter every 48 h. Each data point

represents the mean ± S.D. of four wells. *, cells treated with TGFβ1.

Figure 2. TGFβ induces motility of MCF-10A/HER2 cells. A. Confluent cell monolayers in

serum-free medium were wounded with a pipette tip. Following wounding, TGFβ1 (1 ng/ml)

and/or Herceptin (10 µg/ml) were added as indicated and wound closure was monitored by

microscopy at 18 h. Scale bar: 100 µm. B. A single cell suspension of 2x105 cells in serum-free

medium was seeded onto transwell filters (6-mm diameter, 5-µm pores) in 12-well plates and

allowed to migrate. After 18 h, cells on the underside of the filters were fixed, stained, and

counted. Scale bar: 100 µM. The results are represented quantitatively in the bar graph below.

Each data point represents the mean ± SD of three wells.


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Figure 3. Expression of ALK5T204D enhances motility in MCF-10A/HER2 cells. A. MCF-

10A/vector and MCF-10A/HER2 cells were infected with adenoviruses encoding β-

galactosidase or an HA-tagged constitutively active mutant of TβRI (ALK5T204D) at an MOI of

10 PFU/cell for 1 h. Forty-eight h following infection, HER2 and ALK5 expression was verified

by immunoblot utilizing HER2, HA, TβRI, and actin antibodies. B. Confluent monolayers of

adenovirus-infected cells were wounded with a pipette tip as indicated in Fig. 2A and wound

closure was monitored 18 h later. Scale bar: 100 µm. C. Adenovirus-transduced cells were plated

onto transwell filters as indicated in Fig. 2B. Cell migration to the underside of the transwell

filters was assessed 18 h after plating.

Figure 4. TGFβ-induced motility of HER2-overexpressing MCF-10A cells requires multiple

signaling pathways. A.Wounded monolayers were treated or not with TGFβ1 (1 ng/ml) for 24 h.

BrdU incorporation in situ was determined as indicated in Experimental Procedures. Panel d is

magnified in both proximal (dp) and distal (dd) areas of the wound. BrdU incorporation was

limited to the cells closing the wound. Scale bar: 100 µm. B. Confluent monolayers were

incubated in serum-free medium for 12 h and pretreated with LY294002 (10 µM), SB202190 (10

µM), U-0126 (3 µM), or the integrin β1 blocking antibody (20 ng/ml) for 4 h. Monolayers were

then wounded and TGFβ1 (1 ng/ml) was added. Wound closure was monitored 18 h later. Scale

bar: 100 µM.

Figure 5. TGFβ induces actin reorganization and lamellopodia in MCF-10A/HER2 cells. A.

Fluorescent images of GFP (a,c,e,g) and F-actin (b,d,f,h) in MCF-10A/vector and MCF-

10A/HER2 cells treated or not with TGFβ1 (1 ng/ml) for 15 min. F-actin was visualized with

phalloidin-Texas Red. Panel i represents schematic illustration of panel h. arrow: direction of


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cell movement; *: membrane ruffling and lamellopodia; oval circles: actin stress fibers. Scale

bar: 50 µm. B. Confluent cells in 100-mm dishes were incubated in serum-free medium for 12 h.

Herceptin (10 µg/ml) was then added for 4 h and monolayers were wounded with a pipette tip

followed by treatment with TGFβ1 (1 ng/ml) for 15 min. Cell lysates (800 µg) were prepared

and incubated with immobilized GST-PBD for 3 h. GST-PBD-bound proteins were eluted,

separated by SDS-PAGE, and subjected to Rac1 immunoblot analysis. Top two panels: Whole

cell lysates (50 µg/lane) were subjected to immunoblot analysis with HER2 and Rac1 antibodies.

Bottom panel: Ponceau staining of GST-PBD-bound proteins to document equal loading of GST-

PBD (top) and Rac1 immunoblot of eluates from GST-PBD (bottom). **: Ponceau staining of

GST-PBD to document loading amount. C. Left panel: Cell lysates (50 µg/lane) from MCF-

10A/HER2 cells ± TGFβ1 (2 ng/ml, 15 min) or MCF-10A/HER2 transduced with either β-gal or

ALK5T204D (MOI of 20 PFU/cell, 24 h post-transduction) were subjected to immunoblot analysis

with antibodies for HER2, HA, P-Smad2, total Smad 2/3, Rac1, Pak1, and actin. Right panel:

Cell lysates (200 µg) were precipitated with normal rabbit IgG, Rac1, or Pak1 antibodies.

Immunoprecipitates were subjected to HER2, Rac1, Pak1, and HA immunoblot analyses. An HA

band clearly distinct from the IgG band is apparent in the Rac1 precipitate from ALK5T204D but

not β-gal-transduced cells.

Figure 6. TGFβ enhances Smad-dependent transcription and differential gene expression in

MCF-10A/HER2 cells. A. Cells were transiently transfected with p(CAGA)12-luciferase and

pCMV-Renilla in complete growth medium. The following day, cells were incubated in SFM for

16 h and treated or not with 1 ng/ml TGFβ for additional 24 h. Normalized p(CAGA)12-lux

reporter activity was measured as indicated in Experimental Procedures. Each bar represents the


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mean ± SD relative luciferase activity calculated from 4 wells. B. Differentially expressed genes

in TGFβ-treated (24 h) MCF-10A/HER2 relative to MCF-10A/vector cells. TM, tumor marker;

CC, cell cycle regulatory molecule; CS, cytoskeleton; CK, cytokine or growth factor; TF,

transcription factor; ST, signal transducer; EM, extracellular matrix component. C. Northern

analysis of upregulated TβRII transcript (4.2 kb) and metallothionein 1H transcript (0.37 kb) in

HER2-overexpressing cells. The 4.7- and 1.9-ribosomal RNA units are shown in ethidium

bromide-stained formaldehyde agarose gel immediately above.


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Supplemental Table: raw data from 5K human cDNA array analysis

MCF-10A/Vector: labeled with Cy3MCF-10A/HER2: Labeled with Cy5

Array Location Gene Name Accession # Normalized RatioBlock Column Row Cy5/Cy3

16 12 7 neuronal protein W49799 0.036489 13 7 Homo sapiens cDNA: FLJ22380 fis, clone H W52208 0.037445 6 4 HLA-B associated transcript-3 AA598629 0.04128

17 12 3 E74-like factor 3 (ets domain transcript AA434373 0.041289 13 6 solute carrier family 12 (potassium/chlo AA452841 0.05184

20 14 13 Yamagata-TBD UNK 0.051845 9 1 UNK R22050 0.07776

13 6 3 catalase N54994 0.0806424 13 1 laminin, gamma 1 (formerly LAMB2) H24650 0.0854420 4 1 integrin, beta 4 AA485668 0.088327 4 8 KIAA1134 protein W67514 0.093125 7 8 placenta-specific 1 T67055 0.095041 7 4 multimerin AA423867 0.121922 5 1 integrin, beta 8 W56754 0.121925 8 5 polymerase (RNA) III (DNA directed) (62k AA282063 0.12481 14 7 plexin A2 N91580 0.125761 6 9 hypothetical protein FLJ11181 R63948 0.125769 6 3 acyl-Coenzyme A dehydrogenase, long chai R66006 0.12576

20 4 4 solute carrier family 20 (phosphate tran T55870 0.127686 4 2 RAB11B, member RAS oncogene family H65328 0.13344

10 4 7 kinesin 2 (60-70kD) AA410207 0.1353619 11 4 UNK H99170 0.137284 13 6 steroidogenic acute regulatory protein r AA504615 0.14688

13 6 11 ESTs N72585 0.1526428 14 2 PWP2 (periodic tryptophan protein, yeast H52729 0.1526413 8 4 kinesin 2 (60-70kD) AA410207 0.1574428 4 5 actin, gamma 2, smooth muscle, enteric T60048 0.161286 6 3 sialyltransferase 8 (alpha-N-acetylneura AA169183 0.162247 7 4 interleukin 1 receptor antagonist T72877 0.164169 10 5 guanylate cyclase activator 1A (retina) AA059378 0.16704

13 12 8 CGI-49 protein R00275 0.167046 11 1 tumor-associated calcium signal transduc AA454810 0.16896

24 7 5 FAT tumor suppressor (Drosophila) homolo AA160783 0.1718412 6 6 microtubule-associated protein like echi R27580 0.1737621 7 4 keratin 8 AA598517 0.173767 4 7 UNK T99616 | T996 0.17472

23 13 9 ESTs R68243 0.176641 12 9 FLJ00005 protein R89569 0.17952

20 1 5 EphB3 AA456088 0.1814413 7 9 ESTs T86945 0.182412 12 7 protein kinase, AMP-activated, gamma 2 n H64261 0.185283 12 1 plasminogen activator, tissue AA447797 0.188164 10 6 hypothetical protein hCLA-iso T95823 0.19008

16 13 5 Human mRNA for unknown product, partial H99544 0.191042 13 10 ESTs H47656 0.19392


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9 3 1 small inducible cytokine A4 (homologous H62864 0.19689 3 6 thrombospondin 2 H38240 0.202565 13 3 BTG family, member 2 H69583 0.20352

16 14 2 Human clone 23933 mRNA sequence H56918 0.204486 6 1 DEAD/H (Asp-Glu-Ala-Asp/His) box polypep H27564 0.205446 6 11 retinoic acid receptor responder (tazaro N94424 0.20544

13 4 8 Homo sapiens mRNA; cDNA DKFZp586J0720 (f T97737 0.2054410 2 12 guanine nucleotide binding protein (G pr AA035620 0.2073621 12 8 phosphoglycerate dehydrogenase T98497 0.207365 2 10 EST R95869 0.209281 3 9 ESTs R62835 0.21408

24 14 1 LIM domain kinase 1 H51404 0.215049 5 3 adenosine kinase R12473 0.2162 7 5 filamin A, alpha (actin-binding protein- AA598978 0.21696

21 6 3 cathepsin D (lysosomal aspartyl protease N20475 0.216963 12 6 similar to rat HREV107 AA476438 0.21792

17 7 3 UNK N52533 0.218881 12 6 calcium/calmodulin-dependent protein kin H29322 0.21984

21 12 10 ESTs R27606 0.22083 4 3 actinin, alpha 3 AA196000 0.22176

13 5 11 ESTs N66925 0.2227224 12 1 leukemia inhibitory factor (cholinergic R50354 0.222724 8 8 ESTs, Weakly similar to KIAA0638 protein T83657 0.22464

32 10 6 PPAR(gamma) angiopoietin related protein N98757 0.225613 5 5 thioredoxin reductase beta AA434130 0.227525 2 13 EMPTY EMPTY 0.228489 12 6 secreted frizzled-related protein 4 AA487193 0.22944

11 10 4 cathepsin K (pycnodysostosis) R00859 0.23042 9 5 endoglin (Osler-Rendu-Weber syndrome 1) AA446108 0.232322 13 9 EST R22575 0.233289 5 5 3-hydroxyisobutyryl-Coenzyme A hydrolase AA521228 0.23424

32 13 4 tissue inhibitor of metalloproteinase 2 AA486280 0.234249 11 9 ESTs T99784 0.23616

13 8 3 baculoviral IAP repeat-containing 3 H48533 0.2361613 6 7 ESTs N57936 0.2371217 5 2 platelet-derived growth factor receptor- AA454868 0.239042 12 7 KIAA1481 protein R31785 0.249 11 6 solute carrier family 4, sodium bicarbon AA452278 0.24

13 4 1 Down syndrome critical region gene 1-lik H19440 0.2413 7 12 golgi SNAP receptor complex member 2 R22156 0.240963 5 4 putative T1/ST2 receptor binding protein AA285073 0.24288

24 13 11 ghrelin precursor H66128 0.244813 6 9 UNK R34857 0.246723 6 2 UNK R10293 0.24963 8 3 small inducible cytokine subfamily A (Cy AA495985 0.25152

21 2 8 ESTs T97479 0.252485 2 11 EST N58391 0.25445 1 12 Homo sapiens clone 24505 mRNA sequence R14880 0.2544

13 9 8 ESTs T69732 0.254417 3 1 small nuclear RNA activating complex, po AA043335 0.255361 3 10 ESTs H56331 0.257289 5 2 Rho guanine exchange factor (GEF) 16 AA482070 0.25728


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2 2 10 UNK H94492 0.2582427 1 3 amyloid beta (A4) precursor-like protein H89517 0.2582417 4 3 complement component 3 N93428 0.25927 6 4 interleukin 4 receptor AA293306 0.260161 5 5 BRCA1 associated protein-1 (ubiquitin ca H09066 0.261125 9 11 ESTs N70978 0.26304

13 4 3 chemokine (C-X3-C) receptor 1 N51278 0.2630420 1 12 ribosome binding protein 1 (dog 180kD ho AA464540 0.263042 12 11 ESTs H90280 0.26496

13 5 8 ESTs T66837 0.2649612 10 11 histone deacetylase 7A N57854 0.2659216 8 5 nuclear mitotic apparatus protein 1 AA598659 0.2659225 11 7 Homo sapiens, Similar to DKFZP564M2423 p H53620 0.265921 1 4 NGFI-A binding protein 2 (ERG1 binding p AA434391 0.266887 2 4 mannose phosphate isomerase AA482290 0.26688

21 10 12 ESTs R78533 | R784 0.2668830 6 6 hypothetical protein FLJ12089 AA025779 0.266885 10 12 protein-kinase, interferon-inducible dou R31521 0.26885 1 4 myxovirus (influenza) resistance 2, homo AA286908 0.27072

12 14 7 ubiquitin associated protein T64881 0.2716831 8 1 transforming growth factor, beta 2 AA233738 0.2716813 9 5 Homo sapiens pinch-2 protein mRNA, compl AA460330 0.2726424 14 13 Yamagata-TBD UNK 0.2726420 8 3 guanine nucleotide binding protein (G pr H49592 0.273621 2 11 ESTs N58265 0.27369 11 4 UNK R05416 0.275523 11 7 interleukin 15 receptor, alpha AA054754 0.2784

31 10 6 melanoma adhesion molecule AA497002 0.27841 2 1 KIAA0176 protein R44371 0.27936

12 4 1 insulin-like growth factor binding prote H79047 0.279368 4 2 meningioma (disrupted in balanced transl R59212 0.280329 5 8 KIAA0614 protein T90785 0.280329 8 2 protocadherin alpha 9 AA437139 0.28224

13 9 12 ESTs N63706 0.282244 14 6 mitochondrial ribosomal protein L23 AA453015 0.285127 6 3 CTP synthase H09614 0.28512

21 2 10 DKFZP586N0819 protein R95774 0.2851213 5 1 von Hippel-Lindau syndrome H73054 0.287049 8 3 PRO0195 protein H74004 0.288

12 5 3 splicing factor proline/glutamine rich ( AA425853 0.2881 1 12 forkhead box G1B R44020 0.28896

13 5 10 betaine-homocysteine methyltransferase 2 R98074 0.289923 7 5 cyclin-dependent kinase inhibitor 3 (CDK AA284072 0.29289 3 8 ESTs R02269 0.29289 7 10 UNK H59261 0.29281 1 10 ESTs H54386 0.293765 1 11 ESTs N55074 0.29568

17 5 8 RB-associated KRAB repressor AA074095 0.296641 3 7 SH2-B homolog N94482 0.2976

28 11 5 KIAA0134 gene product AA477623 0.297616 6 11 ESTs, Highly similar to AF219140 1 gastr R62340 0.298566 1 3 transmembrane 4 superfamily member 1 AA487893 0.29952


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13 11 3 TNF receptor-associated factor 1 R71691 0.299521 9 7 hBKLF for basic kruppel like factor R06749 0.300488 11 4 ubiquitin-conjugating enzyme E2H (homolo AA520978 0.30048

21 1 7 ESTs H56670 0.3004826 8 5 Human clone 23933 mRNA sequence H56918 0.300481 1 2 KIAA0431 protein N54389 0.301445 2 3 serine (or cysteine) proteinase inhibito H90764 0.301442 8 1 insulin-like growth factor binding prote AA233185 0.30336

17 7 7 ESTs, Weakly similar to AF116721 61 PRO1 N54594 0.3043217 6 6 hydroxysteroid (17-beta) dehydrogenase 3 AA437291 0.305286 6 2 KIAA0074 protein N54344 0.30624

13 2 3 crystallin, mu R59968 0.306242 10 7 eukaryotic translation initiation factor N95165 0.30725 1 7 Homo sapiens cDNA: FLJ21028 fis, clone C H91308 0.30726 3 4 regucalcin (senescence marker protein-30 H05140 0.30816

10 2 2 fibromodulin AA485748 0.3081613 4 10 ESTs N63853 0.3081615 14 1 solute carrier family 19 (folate transpo R50337 0.3081621 7 9 ESTs N54946 0.3081623 12 7 phosphatidylserine-specific phospholipas H95978 0.308163 10 2 BCL2-like 2 AA454588 0.312

13 11 7 Homo sapiens, Similar to rhotekin, clone W72722 0.3125 1 3 cyclin B1 R46787 0.31296

13 8 11 ESTs N70279 0.3129618 13 2 tight junction protein 2 (zona occludens W31391 0.312962 14 10 ESTs H47864 0.31392

13 3 9 ESTs, Weakly similar to ALUC_HUMAN !!!! R32836 0.3139217 12 6 programmed cell death 4 N71003 0.3139217 3 4 acidic protein rich in leucines AA489201 0.314889 5 1 steroid sulfatase (microsomal), arylsulf H15155 0.315849 4 7 ESTs, Moderately similar to AF141326 1 R T99650 0.31584

13 3 1 zinc finger protein 133 (clone pHZ-13) H17048 0.3158413 1 10 ESTs R95760 0.3158429 1 5 diacylglycerol kinase, zeta (104kD) AA262205 0.3158412 14 13 Yamagata-TBD UNK 0.316825 1 2 purinergic receptor P2X, ligand-gated io R60723 0.31681 1 6 v-ski avian sarcoma viral oncogene homol W69471 0.318721 2 10 EST H53964 0.318729 5 10 Homo sapiens DNA binding peptide mRNA, p N52837 0.31872

12 11 12 ESTs AA427978 0.3187229 2 6 mitogen-activated protein kinase kinase H08749 0.3187213 5 4 MHC class II transactivator AA490920 0.3196813 5 7 Homo sapiens clone FLC0578 PRO2852 mRNA, H70815 0.3225623 3 1 vasodilator-stimulated phosphoprotein AA410429 0.3235224 9 2 discs, large (Drosophila) homolog 1 H24708 0.323525 5 11 ESTs N67041 0.325445 7 11 ESTs N68821 0.325443 1 8 ESTs R01751 0.32641 6 11 EST N90168 0.327363 3 4 aldehyde dehydrogenase 4 family, member H11346 0.327365 8 3 baculoviral IAP repeat-containing 2 R44417 0.327365 4 10 ESTs R97958 0.32736


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13 1 3 cyclin D2 H84154 0.327362 6 3 phosphodiesterase 4B, cAMP-specific (dun AA453293 0.328329 4 2 transmembrane trafficking protein W94609 0.32832

13 7 8 ESTs T67026 0.3283213 7 10 ESTs R98948 0.3283229 11 3 FK506-binding protein 8 (38kD) N95418 0.328329 9 2 protocadherin gamma subfamily A, 8 R34362 0.330245 1 8 ESTs R07632 0.33129 4 10 duodenal cytochrome b N54788 0.3312

15 1 4 UNK AA488073 0.331217 3 9 ESTs R62633 0.3321613 8 1 UNK R70488 0.333125 2 9 ESTs R32723 0.335041 4 10 ESTs H56679 0.336

10 1 7 ESTs T88698 0.33613 3 11 EST N59090 0.336969 14 9 ESTs R02168 0.339848 3 11 fetuin B N70226 0.34085 6 8 GTP binding protein 2 T67069 0.341769 4 6 neutrophil cytosolic factor 1 (47kD, chr AA489666 0.341761 1 7 contactin 1 H20566 0.343685 9 9 ESTs N55558 0.34368

21 1 10 PRO0132 protein H77554 0.3436829 2 10 ESTs R95770 0.343684 6 1 EST H28469 0.34464

17 8 10 ESTs H94035 0.344648 7 12 DNA-dependent protein kinase catalytic s N79761 0.3456

13 13 6 sulfotransferase, estrogen-preferring AA449459 0.345632 10 7 nudix (nucleoside diphosphate linked moi N49686 0.34565 3 4 myosin, light polypeptide 6, alkali, smo AA488346 0.348486 2 10 rcd1 (required for cell differentiation, H68272 0.348482 11 2 aldolase C, fructose-bisphosphate R39463 0.349449 10 9 ESTs H89490 0.34944

26 3 5 chloride channel 3 AA461332 0.3494413 2 1 nel (chicken)-like 2 H45300 0.350420 14 10 ESTs R88747 0.35043 5 2 UNK H75760 0.351363 10 3 DKFZP566C134 protein AA456319 0.351361 10 5 growth arrest-specific 6 AA461110 0.352328 7 2 proprotein convertase subtilisin/kexin t AA455955 0.35232

17 2 3 antigen identified by monoclonal antibod AA426264 0.3532821 1 9 ESTs R31918 0.353282 7 1 interleukin enhancer binding factor 1 R56553 0.354249 1 11 ESTs N74379 0.354249 5 9 EST R63899 0.3552

13 6 8 ESTs T66919 0.35521 1 3 apical protein, Xenopus laevis-like H49455 0.357121 5 12 v-kit Hardy-Zuckerman 4 feline sarcoma v N24824 0.358086 1 7 ESTs T80844 0.358087 4 5 crystallin, beta A1 AA487614 0.358089 8 10 ESTs H59625 0.35808

20 8 4 H1 histone family, member 0 H57830 0.35808


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2 1 10 EHM2 gene H94262 0.3609613 3 2 deformed epidermal autoregulatory factor AA425806 0.3609624 11 4 uridine monophosphate synthetase (orotat AA426227 0.3609629 10 9 ESTs T95088 0.3609628 14 6 TNF receptor-associated factor 4 AA598826 0.361926 4 3 sorting nexin 1 AA449430 0.362886 3 9 Homo sapiens cDNA FLJ13471 fis, clone PL R85805 0.362889 11 2 KIAA0265 protein R67042 0.36288

12 2 12 Homo sapiens 14q32 Jagged2 gene, complet AA463972 0.362883 13 6 G protein pathway suppressor 1 AA521025 0.36384

10 4 12 ALL1 fused gene from 5q31 AA025630 0.3638425 13 5 myeloid cell leukemia sequence 1 (BCL2-r AA488674 0.363841 7 12 v-ski avian sarcoma viral oncogene homol W69471 0.3648

13 12 1 transcobalamin I (vitamin B12 binding pr AA155640 0.364813 13 8 hypothetical protein MGC5627 H02336 0.364813 6 10 ESTs R98767 0.36483 13 3 imprinted in Prader-Willi syndrome H93310 0.367689 7 7 EST N75993 0.367689 4 8 ESTs R02546 0.367682 13 7 serine (or cysteine) proteinase inhibito T80849 0.36864

17 6 2 ESTs H43855 0.368641 1 8 ESTs R01127 0.3696

25 13 4 brain-derived neurotrophic factor AA262988 0.36965 8 7 ESTs, Weakly similar to ALU1_HUMAN ALU S W72814 0.370569 1 9 ESTs R96490 0.37056

17 4 8 ESTs R02390 0.3705621 2 9 ESTs, Weakly similar to transformation-r R76408 0.370563 11 3 CHK1 (checkpoint, S.pombe) homolog N53057 0.37152

13 4 2 pyruvate dehydrogenase kinase, isoenzyme H29475 0.3715213 8 8 Homo sapiens, clone IMAGE:3448343, mRNA, T69726 0.371524 8 7 hypothetical protein FLJ11198 H59609 0.372489 2 8 ESTs R02017 0.37344

17 10 8 KIAA1436 protein T87069 0.373449 1 1 KIAA0193 gene product AA018659 0.3744

20 10 9 stanniocalcin 2 R20779 0.37443 5 8 oxysterol binding protein-like 1 N54265 0.37536

17 2 5 eyes absent (Drosophila) homolog 3 AA262504 0.3753626 6 10 ESTs R01192 0.375362 12 6 Human mRNA for unknown product, partial N21309 0.376324 1 12 tetraspan 5 R98344 0.37632

17 1 8 splicing factor, arginine/serine-rich 5 N38809 0.3763228 14 8 ESTs H66470 0.3763219 13 12 EST N74456 0.3772830 4 10 ESTs N52398 0.377285 8 4 kinesin family member 5B AA046690 0.37824

20 11 8 HP1-BP74 T90920 0.3782429 5 2 RNA binding motif protein 5 W73892 0.3782421 4 3 chloride channel 4 AA019316 0.3801610 14 8 Homo sapiens mRNA; cDNA DKFZp434P1115 (f R12671 0.3811212 3 4 lysophospholipase-like T60135 0.3830417 9 1 ESTs H00677 0.3830417 3 12 EST H11658 0.38304


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20 3 5 general transcription factor IIF, polype AA282092 0.383043 7 4 interleukin enhancer binding factor 3, 9 AA449762 0.3847 7 10 EST R99584 0.3849 3 12 ret proto-oncogene (multiple endocrine n H24956 0.384

12 5 7 Homo sapiens cDNA FLJ13408 fis, clone PL N63941 0.38413 3 5 KIAA0513 gene product H24428 | H244 0.38421 2 1 nel (chicken)-like 1 N79534 0.3841 1 1 Homo sapiens mRNA; cDNA DKFZp564F093 (fr H70498 0.38592

13 5 9 myotubularin related protein 4 H70612 0.3859217 7 2 protein tyrosine phosphatase, receptor t AA464590 0.3859224 11 2 frizzled-related protein W58032 0.385921 3 8 ESTs R02313 0.387849 5 7 Glutamate receptor interacting protein R36143 0.387846 14 4 transferrin receptor (p90, CD71) AA487593 0.390729 2 10 ESTs H53950 0.39072

10 3 2 fibrillin 2 (congenital contractural ara T98152 0.3916825 13 6 ESTs, Highly similar to Z169_HUMAN ZINC T88890 0.391682 11 6 Homo sapiens cDNA FLJ11801 fis, clone HE R99346 0.3936

21 5 7 UNK T64921 0.393613 10 4 mitogen-activated protein kinase 4 AA400982 0.3945616 11 2 fragile X mental retardation, autosomal AA489628 0.3945617 1 2 nuclear factor (erythroid-derived 2), 45 H58953 0.394561 4 7 Down syndrome critical region gene 1-lik R26919 0.395522 10 11 UNK R76909 0.39552

24 14 2 Human clone A9A2BR11 (CAC)n/(GTG)n repea AA233365 0.395526 2 5 hepatocyte growth factor (hepapoietin A; R52798 0.39648

13 9 1 transcobalamin II; macrocytic anemia AA490459 0.3964817 3 5 postmeiotic segregation increased 2-like AA459266 0.3964826 9 2 cAMP response element-binding protein CR R22790 0.396484 1 3 prolactin AA130238 0.397445 9 13 EMPTY EMPTY 0.39744

13 7 2 complement component 3a receptor 1 AA464711 0.397442 11 8 hypothetical protein MGC3260 R94946 0.39849 11 8 ESTs H80994 0.39846 5 4 KIAA0367 protein AA447773 0.399369 10 3 UNK R86721 0.39936

13 13 11 ESTs R93007 0.3993621 11 7 hypothetical protein FLJ23591 H63402 0.399361 9 4 major histocompatibility complex, class H42728 0.40032

25 8 8 ESTs R99527 0.400325 3 10 KIAA0220 protein R97226 0.401289 12 7 hypothetical protein FLJ21620 R00226 0.40128

12 1 6 calcium-regulated heat-stable protein (2 W72753 0.4012814 3 2 transcription factor AP-2 beta (activati AA018906 0.401284 13 1 RAP2A, member of RAS oncogene family W32474 0.40224

14 6 8 ESTs T69754 0.4022420 12 12 ESTs, Highly similar to thyroid hormone R89581 0.4022422 9 10 ESTs R67081 0.4022413 11 1 EphA2 H84481 0.403213 8 2 proprotein convertase subtilisin/kexin t AA256502 0.403213 8 6 UNK AA598508 0.403228 12 10 beta3gal-T5 gene H93550 0.40416


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29 3 7 GTF2I repeat domain-containing 1 AA019591 0.404161 13 3 GDNF family receptor alpha 2 H05619 0.40512

17 5 3 adenosine monophosphate deaminase (isofo R01733 0.405123 13 7 ribosomal protein S6 kinase, 90kD, polyp R43585 0.406089 4 4 serine palmitoyltransferase, long chain AA158255 0.40608

17 4 9 hypothetical protein FLJ13910 R63731 0.406082 3 8 UNK R73876 0.407049 10 6 CD27-binding (Siva) protein AA167728 0.40704

16 6 3 PCTAIRE protein kinase 1 AA283125 0.4070427 10 12 type I transmembrane protein Fn14 R33355 0.4070417 4 6 neurotrimin AA425422 0.40831 6 6 collagen, type VI, alpha 2 AA464748 0.4083 2 5 TNF receptor-associated factor 3 AA504259 0.40896

13 4 9 EST R33665 0.408962 11 9 hypothetical protein FLJ22351 W74436 0.40992

29 10 5 Purkinje cell protein 4 AA452826 0.4099222 8 12 UNK T99017 | T990 0.4108829 2 3 UNK H82536 0.4108832 14 13 Mu-Cox-2 Mu-Cox-2 0.410889 1 8 ESTs R01115 0.41184

13 1 7 spindlin R26696 0.4118420 13 11 Homo sapiens, clone IMAGE:3546959, mRNA, H91632 0.411848 5 6 UNK N62553 0.4128

12 14 1 quiescin Q6 AA464152 0.412813 3 8 Homo sapiens clone IMAGE:121687 mRNA seq T97628 0.412817 4 10 ESTs H56438 0.412825 3 1 small proline-rich protein 1B (cornifin) AA447684 0.412828 8 7 KIAA1085 protein H96878 0.41282 5 7 B7 homolog 3 N54338 0.41376

23 1 1 UDP-glucose ceramide glucosyltransferase AA456051 0.413765 4 5 phosphatidylinositol binding clathrin as AA441930 0.41568

25 4 12 B-cell CLL/lymphoma 2 H74208 0.416641 1 5 N-myc (and STAT) interactor AA279762 0.4176

17 12 5 inhibitor of DNA binding 4, dominant neg AA464856 0.41766 9 3 H1 histone family, member 2 T66815 0.41856

25 11 11 ESTs H48255 0.4185612 5 12 ESTs N34751 0.4195223 14 9 ESTs R00495 0.4195220 3 2 ESTs H63361 0.420489 6 2 leucine-zipper-like transcriptional regu R38194 0.42144

27 2 12 Homo sapiens BCL-2-related ovarian kille R87777 0.4214429 8 1 plexin A1 R17676 0.421442 1 5 surfeit 5 AA459247 0.42243 5 6 proteasome (prosome, macropain) subunit, AA620580 0.42243 6 11 hypothetical protein FLJ22357 similar to W69154 0.42245 7 7 EST N68163 0.4224

16 11 12 kinesin family member 13B W86466 0.422417 3 2 PAX transcription activation domain inte R22179 0.422413 6 13 EMPTY EMPTY 0.4233631 2 6 DEAD/H (Asp-Glu-Ala-Asp/His) box polypep AA458801 0.4233613 11 9 ESTs T95327 0.4243228 10 2 armadillo repeat gene deletes in velocar H17975 0.42624


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1 7 9 ESTs, Weakly similar to ALU1_HUMAN ALU S H90887 0.428161 5 10 EST H56926 0.42816

13 7 3 protein tyrosine phosphatase, receptor t N49751 0.4281625 9 1 RAB4, member RAS oncogene family H93459 0.4281611 14 10 ESTs R66991 0.4300813 2 12 ESTs T81574 0.430081 5 7 hypothetical protein DKFZp434M0331 N58405 0.432968 1 3 syndecan 4 (amphiglycan, ryudocan) AA148737 0.43296

12 7 8 Homo sapiens mRNA for KIAA1727 protein, H56655 0.4329621 5 4 major histocompatibility complex, class T63324 0.432967 7 1 transforming growth factor, beta recepto H62473 0.43392

13 3 10 ESTs, Moderately similar to ALU1_HUMAN A R97032 0.4348810 4 5 glutathione peroxidase 2 (gastrointestin AA135152 0.4358414 9 2 solute carrier family 20 (phosphate tran W46972 0.435841 2 11 ESTs, Moderately similar to JC5604 ABC-t R07617 0.4368

17 7 5 cadherin 12, type 2 (N-cadherin 2) AA418564 0.43688 8 1 myosin VA (heavy polypeptide 12, myoxin) AA025850 0.437769 1 3 apolipoprotein B (including Ag(x) antige H93332 0.43776

25 3 8 ESTs, Weakly similar to ALU6_HUMAN ALU S R08359 0.437766 10 4 UNK N58107 0.43872

17 4 11 KIAA0966 protein R69354 | R693 0.438728 10 7 thioredoxin reductase beta R45108 0.43968

10 2 8 ESTs R22988 0.4396828 10 7 ESTs, Highly similar to A34924 complemen T66823 0.439682 13 12 hypothetical protein R65573 0.44064

29 4 9 EST R33616 0.4406413 1 8 ESTs, Weakly similar to ALUB_HUMAN !!!! T96965 0.441623 12 9 ESTs, Highly similar to AF116721 101 PRO T96123 0.44164 6 2 checkpoint suppressor 1 H84982 0.442566 2 7 ESTs T81034 0.442569 2 9 ESTs R71191 0.442563 5 1 Ric (Drosophila)-like, expressed in neur N53351 0.44352

13 10 1 serine (or cysteine) proteinase inhibito T64901 0.443526 8 3 myosin light chain 2a N93941 0.44448

23 13 7 phosphodiesterase 6D, cGMP-specific, rod N62658 0.4444816 11 8 Homo sapiens cDNA FLJ13557 fis, clone PL R09218 0.4454426 11 1 transglutaminase 2 (C polypeptide, prote R97066 0.445442 7 7 lipase, hormone-sensitive H50238 0.44649 2 3 annexin A11 AA464982 0.447365 11 5 splicing factor, arginine/serine-rich 3 AA598400 0.448329 7 2 chromosome 6 open reading frame 32 H57941 0.448329 4 5 Homo sapiens Cri-du-chat region mRNA, cl H14816 0.44832

17 2 1 KIAA0180 protein T91958 0.4483217 1 3 apolipoprotein C-II AA454580 0.4483226 4 2 ferrochelatase (protoporphyria) T64893 0.4483232 12 6 zinc finger protein 258 AA280677 0.4483213 14 8 ESTs, Weakly similar to A49134 Ig kappa N62648 0.4492816 14 7 sorting nexin 8 AA026689 0.4492826 6 11 ESTs N59772 0.4502413 12 7 ESTs, Moderately similar to E2BA_HUMAN T N73898 0.451229 1 3 cystathionase (cystathionine gamma-lyase R07167 0.451213 4 11 EST N59800 0.45216


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25 8 2 KIAA0350 protein H15077 0.4521618 4 6 ESTs H59208 0.453123 1 2 BCL2/adenovirus E1B 19kD-interacting pro AA063521 0.454088 7 5 gp25L2 protein AA489314 0.454088 14 7 ESTs, Moderately similar to 2208306A met H79507 0.454089 6 6 trophinin associated protein (tastin) H94949 0.45408

25 3 7 hypothetical protein FLJ13195 similar to R08261 0.454085 3 9 ESTs R32997 0.45504

28 9 4 ribonuclease/angiogenin inhibitor R88243 0.4550428 13 12 ESTs R22052 0.4562 8 5 erythrocyte membrane protein band 4.2 AA449835 0.456969 13 5 nuclear receptor subfamily 4, group A, m AA598611 0.45696

13 7 11 ESTs N80160 0.4569629 6 1 uncoupling protein 3 (mitochondrial, pro AA192553 0.456961 3 11 UNK R11510 0.457925 1 5 cyclin-dependent kinase 4 AA486208 0.45792

31 4 3 cartilage linking protein 1 AA115902 0.457927 1 4 mesoderm specific transcript (mouse) hom AA598610 0.458887 1 8 ESTs T97327 0.45888

16 12 4 transcription elongation factor B (SIII) AA133129 0.4588823 14 8 GTPase Rab14 T66955 0.46081 14 10 ESTs N59650 0.461767 3 6 cytochrome P450, subfamily IIB (phenobar T68287 0.461769 12 11 EST H48793 0.46176

17 2 7 prohibitin R60946 0.4617625 1 11 UNK N74366 0.461762 6 10 ESTs H72932 0.462725 1 10 Homo sapiens mRNA; cDNA DKFZp761G151 (fr R95765 0.46272

13 13 7 cofactor required for Sp1 transcriptiona N92735 0.4627222 10 3 discoidin domain receptor family, member AA243749 0.4627217 3 8 EST, Weakly similar to T00415 hypothetic R02261 0.463684 13 13 EMPTY EMPTY 0.46464

18 3 10 ubiquitin specific protease 12 H97566 0.4646413 7 7 down-regulated in metastasis N58318 0.4665619 9 4 CD3G antigen, gamma polypeptide (TiT3 co T66799 0.4665621 5 10 ESTs H94484 0.466561 5 6 interferon, alpha-inducible protein (clo AA448478 0.467529 7 9 ESTs T98266 0.467529 6 10 ESTs N59645 0.46752

11 12 11 UNK H52412 0.467524 2 3 presenilin 2 (Alzheimer disease 4) AA450249 0.46848

32 12 7 UNK R19189 0.469449 4 9 ring finger protein 2 R63802 0.4704

26 13 9 Down syndrome critical region gene 4 R21725 0.470417 6 7 ESTs R02166 0.4713617 2 8 ESTs R01899 0.471365 10 9 ESTs T95262 0.472327 3 9 ESTs R36650 0.472328 12 8 ESTs N57728 0.472322 14 12 Leman coiled-coil protein R67355 0.47328

17 10 9 Homo sapiens mRNA full length insert cDN R19403 0.4742417 2 11 ESTs, Moderately similar to ALUF_HUMAN ! T90962 0.47424


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29 3 12 Homo sapiens cDNA FLJ11723 fis, clone HE T84084 0.474246 13 8 interferon consensus sequence binding pr N62269 0.47525 3 11 UNK N68161 0.476164 9 1 trans-Golgi network protein (46, 48, 51k H82891 0.477124 5 3 UNK T62075 0.477124 11 6 ESTs, Weakly similar to unnamed protein N54398 0.477125 5 3 cell division cycle 27 T81764 0.477122 14 5 KIAA0089 protein AA485401 0.478085 2 5 phosphatidic acid phosphatase type 2B T71976 0.47808

13 8 5 ribonuclease P (30kD) AA458957 0.4780814 7 6 ornithine decarboxylase antizyme 2 T67028 0.478087 6 7 ESTs, Weakly similar to ALU1_HUMAN ALU S T77891 0.47904

24 10 12 similar to Bos taurus P14 protein AA458464 0.4790428 9 13 EMPTY EMPTY 0.479045 12 3 polymerase (DNA directed), delta 2, regu AA428377 0.488 12 3 small inducible cytokine subfamily A (Cy R96626 0.48096

17 9 12 baculoviral IAP repeat-containing 3 AA002126 0.4819231 10 4 diphtheria toxin receptor (heparin-bindi R45640 0.4819217 8 8 a disintegrin-like and metalloprotease ( R76553 | R762 0.4828825 1 7 Homo sapiens ryanodine receptor 2 (cardi R15791 0.4828829 2 11 EST N57896 0.482881 4 1 selectin L (lymphocyte adhesion molecule H00662 0.48384

20 11 5 UNK N68825 0.483841 4 8 EST R02550 0.4848

29 14 11 ESTs R94725 0.48489 4 3 alanine-glyoxylate aminotransferase homo N57872 0.485763 6 6 protein-L-isoaspartate (D-aspartate) O-m T68453 0.487687 5 7 ESTs, Weakly similar to ALU1_HUMAN ALU S R06894 0.48768

25 3 4 four and a half LIM domains 1 AA455925 0.487686 10 12 C3H-type zinc finger protein; similar to W16832 0.48864

13 3 3 complement component 8, alpha polypeptid H53865 0.4886413 2 4 myotubular myopathy 1 AA491225 0.4886421 7 11 ESTs N68671 0.4886426 4 12 potassium inwardly-rectifying channel,su H97186 0.488643 5 5 TATA box binding protein (TBP)-associate H87496 0.48968 14 10 Homo sapiens brain my038 protein mRNA, c R76457 0.48969 9 9 Homo sapiens cDNA FLJ11872 fis, clone HE T98661 0.4896

21 3 12 ESTs R06588 0.48965 2 12 ESTs T81310 0.49056

23 13 8 Homo sapiens, pre-mRNA splicing factor s H82227 0.4905627 11 11 alpha integrin binding protein 63 H50840 0.4905628 11 1 HIV-1 Tat interactive protein, 60 kDa AA017043 0.490562 13 1 guanine nucleotide binding protein (G pr N68166 0.491523 13 2 dedicator of cyto-kinesis 2 N70765 0.49152

12 11 1 symplekin; Huntingtin interacting protei W74377 0.4915221 1 8 splicing factor, arginine/serine-rich 2, T96952 0.4915229 9 8 ESTs T69711 0.4915217 1 7 aldehyde dehydrogenase 4 family, member H11346 0.492488 6 11 HIV-1 rev binding protein 2 W52273 0.493449 9 10 ESTs R36669 0.49344

13 4 5 Homo sapiens pTM5 mariner-like transposo T53022 0.4934432 8 6 ESTs, Weakly similar to ORF2-like protei R19287 0.49344


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10 14 6 major vault protein AA158991 0.494419 11 7 ESTs, Moderately similar to CALD_HUMAN C H48508 0.494428 11 8 ESTs T90915 0.494413 5 12 EST H57816 0.4953629 12 6 coagulation factor XIII, A1 polypeptide AA449742 0.495363 2 10 ESTs H60120 0.496327 13 1 solute carrier family 4, anion exchanger T86708 0.49632

11 11 11 ESTs H52104 0.4963212 14 11 ESTs, Weakly similar to ALUB_HUMAN !!!! N55008 0.4963216 11 5 a disintegrin and metalloproteinase doma AA292676 0.4963220 4 12 ESTs W95104 0.4963224 11 12 Sarcolemmal-associated protein W37882 0.4963225 12 5 inositol polyphosphate phosphatase-like AA279072 0.4963231 2 3 collagen, type V, alpha 1 R75635 0.4963215 3 3 adaptor-related protein complex 2, beta N52267 0.4972827 14 11 ESTs N73768 0.497285 9 8 step II splicing factor SLU7 T70344 0.498247 1 7 ESTs, Weakly similar to T00377 KIAA0642 R34240 0.49824

17 4 4 sialyltransferase AA497051 0.498244 4 12 hypothetical protein FLJ11016 H59837 0.4992

13 2 10 ESTs R95852 0.499226 10 11 ESTs R22198 0.49921 4 2 collagen, type VI, alpha 3 R62603 0.50016

30 4 6 EST T96855 0.5001631 2 5 hypothetical protein FLJ10871 AA447959 0.5001611 14 3 Homo sapiens GT212 mRNA H82977 0.5011220 7 10 UNK N52568 0.5011229 10 7 ESTs, Moderately similar to LCP2_HUMAN L N52958 0.501126 14 3 Ran GTPase activating protein 1 AA026631 0.502089 3 10 ESTs H56127 0.50208

25 2 2 delta (Drosophila)-like 1 R41685 0.502086 1 10 ESTs H68523 0.50304

17 6 8 hypothetical protein FLJ20392 T77893 0.5030417 7 8 ESTs N73550 0.5030421 7 10 UNK R98947 0.5030429 2 8 ESTs H77603 0.503041 1 11 ESTs N74386 0.5045 12 12 ESTs, Weakly similar to KIAA1353 protein W80964 0.504969 8 9 ESTs T98484 0.50496

19 11 11 ESTs H94978 0.5049624 12 8 UNK H98001 0.5049629 7 11 ESTs N68607 0.504965 5 6 crystallin, alpha B AA504891 0.50592

13 8 7 ESTs, Weakly similar to ALU2_HUMAN ALU S H61037 0.5059220 5 12 ESTs, Weakly similar to ALU1_HUMAN ALU S H71224 0.505921 3 12 hypothetical protein FLJ23306 T58873 0.506882 3 3 proprotein convertase subtilisin/kexin t AA069517 0.506885 3 8 ESTs, Weakly similar to ALU8_HUMAN ALU S N72576 0.506886 13 3 UNK R68237 0.50688

13 11 8 vav 2 oncogene H54025 0.5068813 6 12 EST H78384 0.5068820 5 11 hypothetical protein FLJ23311 N73916 0.50688


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7 7 3 host cell factor C1 (VP16-accessory prot R69885 0.5078412 4 9 ESTs N54407 0.5078422 9 4 zinc finger protein 42 (myeloid-specific AA279941 0.5078428 10 8 pan-hematopoietic expression N49723 0.507841 2 7 DKFZP434C212 protein R00841 0.50884 2 6 cysteine-rich motor neuron 1 R70508 0.50889 13 3 syntrophin, beta 1 (dystrophin-associate R10726 0.5088

10 2 7 hypothetical protein N58503 0.50884 7 4 HIV-1 rev binding protein 2 AA251800 0.50976

17 6 9 A kinase (PRKA) anchor protein 11 R63925 0.5097626 14 8 ESTs T91287 0.5097625 13 12 ESTs N73986 0.510728 11 10 ESTs R68409 0.51168

13 6 6 connective tissue growth factor AA598794 0.5116814 12 6 CTL2 gene R39578 0.5116824 11 8 ESTs R97773 0.5116812 6 2 chitinase 1 (chitotriosidase) T94579 0.5126416 13 8 ESTs, Weakly similar to ALU1_HUMAN ALU S H69787 0.512644 14 10 UNK R89583 0.51369 2 6 v-raf-1 murine leukemia viral oncogene h N25425 0.5136

12 12 12 Homo sapiens HT036 (HT036) mRNA, complet N95780 0.513625 10 6 RNA binding motif protein 10 T68092 0.5145629 14 8 syntaxin 11 R33852 0.5145630 13 10 ESTs R69798 0.514563 8 12 BCL2/adenovirus E1B 19kD-interacting pro AA446839 0.515529 13 12 ESTs N74073 0.51552

13 2 8 Homo sapiens clone DT1P1B6 mRNA, CAG rep H57718 0.5155222 1 7 ESTs N69298 0.5155225 9 5 calpain 6 AA457238 0.5155213 1 1 phospholipase C, epsilon AA406325 0.5164818 2 8 insulin receptor tyrosine kinase substra R22950 0.5164813 4 12 ESTs R91034 0.5174425 1 6 zinc finger protein 143 (clone pHZ-1) AA443659 0.5174427 11 9 ESTs R00138 0.5174415 2 1 RAN binding protein 2 H78788 0.518412 4 12 ESTs, Weakly similar to ubiquitous TPR m N90564 0.5193613 3 12 EST T90991 0.5193617 8 9 KIAA1319 protein N70196 0.5193619 12 8 hypothetical protein FLJ20079 R99573 0.5193621 9 8 ESTs T70318 0.519364 8 12 ESTs, Moderately similar to T09073 splic R91171 0.520326 7 7 transcription factor AP-2 alpha (activat R38044 0.52032

13 9 3 RNA binding motif protein 4 AA454656 0.5203213 7 6 chromosome condensation 1 AA291398 0.5203223 7 8 EST T67452 0.5203221 8 11 ESTs N70261 0.5222421 10 11 ESTs N71010 0.5222427 12 9 ESTs R00217 0.522243 6 1 polymerase (RNA) II (DNA directed) polyp AA430656 0.5232

16 1 11 ESTs H72592 0.523229 3 11 hypothetical protein FLJ12057 N58398 0.52325 5 7 ESTs T98514 0.52416


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29 4 3 cholinergic receptor, nicotinic, epsilon R02059 0.5241610 1 1 Ras-related associated with diabetes W84445 0.5251229 13 11 ESTs N49893 0.5251232 7 3 heterogeneous nuclear ribonucleoprotein T97593 0.5251223 12 11 M-phase phosphoprotein 9 R94533 0.5260820 14 2 amplified in osteosarcoma AA013242 0.5270427 1 2 CASP8 and FADD-like apoptosis regulator AA453766 0.5270417 13 2 lipin 2 T95515 0.52824 14 12 serine (or cysteine) proteinase inhibito T82264 0.52825 7 2 D15F37 (pseudogene) N58136 0.52827 11 12 ESTs H23963 0.5281 6 7 ESTs R09487 0.528965 2 7 ESTs N49768 0.528969 7 11 protein kinase, Y-linked N99154 0.52896

12 3 8 integrin, beta 8 R74358 0.5289621 6 12 tubby super-family protein H78234 0.528961 3 1 small inducible cytokine A2 (monocyte ch AA425102 0.529921 8 10 ESTs H59922 0.52992

10 6 9 Homo sapiens cDNA: FLJ21199 fis, clone C R92181 0.5299225 10 10 UNK R36539 0.5299228 12 9 KIAA1538 protein N63748 0.529922 1 9 Homo sapiens clone FLC0675 PRO2870 mRNA, R89084 0.530889 11 7 regulatory solute carrier protein, famil H02333 0.530889 6 13 EMPTY EMPTY 0.53088

11 3 4 regulator of G-protein signalling 7 H24326 0.5308817 3 10 ESTs H56653 0.5308824 8 11 ESTs, Weakly similar to ALU1_HUMAN ALU S T97590 0.5308829 3 6 X-ray repair complementing defective rep AA425139 0.530882 14 4 pyruvate dehydrogenase (lipoamide) beta AA521401 0.531844 12 13 EMPTY EMPTY 0.53184

13 8 12 hypothetical protein FLJ20602 R32658 0.532817 7 4 protein kinase C binding protein 1 AA480906 0.532821 14 5 neogenin (chicken) homolog 1 AA447658 0.532825 9 3 src kinase-associated phosphoprotein of R01170 0.53281 6 5 RNA-binding protein S1, serine-rich doma AA496879 0.533762 9 10 Homo sapiens cDNA FLJ10641 fis, clone NT R87101 0.53376

15 10 5 lethal giant larvae (Drosophila) homolog AA026112 0.5337621 11 12 ESTs R08563 0.5337626 4 5 growth factor receptor-bound protein 2 AA449831 0.5337629 2 5 Homo sapiens cDNA: FLJ22488 fis, clone H R86843 0.533767 3 12 ESTs R74041 0.53472

13 12 6 cyclic nucleotide gated channel alpha 1 W93472 0.5347217 14 6 SWI/SNF related, matrix associated, acti AA446018 0.534727 9 3 G protein-coupled receptor R91916 0.53568

14 4 6 hypothetical protein FLJ20363 R32892 0.5356817 13 4 carcinoembryonic antigen-related cell ad AA406571 0.5356817 7 10 UNK H59254 0.5356820 8 11 Homo sapiens mRNA; cDNA DKFZp434H1322 (f R35849 0.5356818 6 4 flightless I (Drosophila) homolog AA521453 0.5366424 14 8 ESTs R26784 0.537627 13 9 ESTs R01220 0.53763 2 8 KIAA0648 protein R02821 0.53856


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21 8 9 ESTs N64734 0.5385621 8 10 ESTs N54161 0.5385631 5 2 protocadherin gamma subfamily C, 3 R89615 0.538569 2 7 pyruvate dehydrogenase (lipoamide) alpha R49470 0.53952

27 13 11 ESTs H53317 0.5395230 12 10 Homo sapiens mRNA full length insert cDN R68627 0.539521 13 12 ESTs N74210 0.540489 4 1 sex hormone-binding globulin T69271 0.540489 6 7 ESTs R01706 0.54048

26 2 6 similar to phosphatidylcholine transfer R76230 0.5404831 1 5 Human clone 23908 mRNA sequence H09540 0.540485 1 9 Homo sapiens PAK2 mRNA, complete cds R32035 0.541442 9 11 ESTs R66946 0.54248 7 6 Homo sapiens ubiquitin protein ligase (U R06429 0.5424

21 1 11 ESTs N54811 0.542424 4 1 proteasome (prosome, macropain) subunit, T54166 0.542424 12 5 rcd1 (required for cell differentiation, AA488188 0.542417 1 11 ESTs N74377 0.543364 5 10 ESTs N39562 0.544328 2 10 ESTs H69695 0.54432

13 9 6 Homo sapiens clone 24651 mRNA sequence AA487560 0.5443227 11 8 Homo sapiens cDNA: FLJ23313 fis, clone H R85387 0.5443229 10 8 ESTs T70353 0.5443212 1 5 epididymis-specific, whey-acidic protein AA451904 0.545281 2 6 v-raf murine sarcoma 3611 viral oncogene H59758 0.54624

28 5 2 chromosome segregation 1 (yeast homolog) N69204 0.5462430 13 11 ESTs H63237 0.546244 1 7 ESTs H52534 0.54725 4 9 ESTs R34271 0.54728 4 6 EST R36081 0.5472

24 13 3 mitogen-activated protein kinase-activat AA455056 0.547231 3 4 lysyl oxidase AA452916 0.54721 5 11 hypothetical protein FLJ21272 H80860 0.548169 9 4 colipase, pancreatic T54662 0.54816

21 2 12 ESTs T75482 0.5481626 12 12 NY-REN-18 antigen N29431 0.548163 11 1 protein kinase C, alpha AA029890 0.54912

13 4 7 ESTs, Weakly similar to A35802 15-hydrox AA011185 0.549122 10 5 ets variant gene 5 (ets-related molecule AA460265 0.550085 8 12 hypothetical protein MGC5576 T96608 0.550086 2 9 small nuclear RNA activating complex, po W15452 0.55008

31 2 7 hypothetical protein FLJ12443 R97014 0.550083 11 4 carbamoyl-phosphate synthetase 2, aspart R85414 0.551045 3 3 complement component 6 N59396 0.551049 14 10 ESTs H25020 0.55104

12 13 10 thrombospondin 1 H80424 0.5510416 2 9 ESTs AA029362 0.5510420 11 11 UNK H90434 0.5510427 14 12 ESTs N74927 0.551049 8 7 KIAA0557 protein R22054 0.552

26 11 9 ESTs N70015 0.55226 14 9 H.sapiens gene from PAC 106H8 R22598 0.552


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1 3 5 MAP-kinase activating death domain AA281945 0.552966 2 11 N-acylsphingosine amidohydrolase (acid c W47576 0.552961 9 9 aldehyde dehydrogenase 8 family, member N70701 0.55392

20 4 6 translocase of outer mitochondrial membr R94191 0.5539220 9 6 mannose-P-dolichol utilization defect 1 R77432 0.5539221 5 12 ESTs R94633 0.5539231 2 11 EST N62467 0.553923 11 2 zinc finger protein 142 (clone pHZ-49) AA443888 0.55488

32 7 13 EMPTY EMPTY 0.554885 3 7 hypothetical protein FLJ14297 H91281 0.555845 7 12 ATP-dependent RNA helicase W73792 0.55584

13 9 2 phosphatidylinositol (4,5) bisphosphate R94222 0.5558416 1 12 hypothetical protein FLJ22378 AA454831 0.5558421 9 2 phosphatidylinositol-4-phosphate 5-kinas H80263 0.5558421 6 11 ESTs, Highly similar to PTD015 [H.sapien N68271 0.5558422 11 10 ESTs R66586 0.5558425 8 7 UNK R02591 0.5558426 9 12 UNK AA151930 0.5558412 2 7 ESTs H93010 0.556816 1 9 ESTs N62384 0.556821 4 9 ESTs R67211 0.55686 5 10 ESTs H72588 0.55776

29 3 3 contactin 1 R38995 0.557764 6 12 ESTs, Weakly similar to AF161361 1 HSPC0 R88764 0.55872

14 1 3 HIR (histone cell cycle regulation defec H23460 0.5587230 11 8 ESTs N52397 0.558722 6 9 ESTs R92188 0.559688 5 12 MKP-1 like protein tyrosine phosphatase AA136040 0.559689 12 8 basement membrane-induced gene N93440 0.55968

25 4 11 hypothetical protein FLJ20624 R26068 0.5596828 9 3 natural killer cell group 7 sequence T57859 0.5596817 8 11 UNK T97592 0.5606427 4 12 zinc finger protein 162 N46360 0.561630 9 10 ESTs R66103 0.56161 3 3 ankyrin 1, erythrocytic AA464755 0.56256

14 2 5 UNK T51849 0.5625620 4 7 hypothetical protein KIAA1259 R01452 0.5625630 12 1 ATP-binding cassette, sub-family B (MDR/ AA455911 0.5625630 11 2 N-deacetylase/N-sulfotransferase (hepara AA428321 0.5625631 1 4 murine leukemia viral (bmi-1) oncogene h AA478036 0.562561 12 11 ESTs H58945 0.56352

11 13 8 HSPC055 protein H73265 0.5635213 9 9 ESTs, Moderately similar to ALU7_HUMAN A R08153 0.5635217 6 11 ESTs N52831 0.5635221 8 7 UNK R91386 0.5635230 12 5 adaptor-related protein complex 3, beta H11603 0.5635212 4 6 GTP-binding protein H94877 0.5644831 6 4 junction plakoglobin R06417 0.564486 10 2 interleukin 11 receptor, alpha AA454657 0.56544

21 7 7 ESTs, Weakly similar to ALU1_HUMAN ALU S H70143 0.5654426 7 11 KIAA1229 protein R86953 0.566429 6 3 cathepsin E H94487 0.5664


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20 5 4 UNK AA486221 0.5673620 12 10 ESTs H53839 0.5673625 4 7 Fas (TNFRSF6) associated factor 1 W70062 0.567361 3 4 inositol polyphosphate-5-phosphatase, 14 AA521067 0.568322 12 12 Homo sapiens cDNA: FLJ21686 fis, clone C R14869 0.568324 9 9 KIAA0615 gene product R16998 0.568325 2 8 ESTs T97586 0.568326 2 3 stress-induced-phosphoprotein 1 (Hsp70/H AA487427 0.56832

20 2 13 EMPTY EMPTY 0.5683226 11 3 glutathione S-transferase pi R33642 0.568325 4 11 ESTs H50638 0.569286 10 3 chloride channel 6 H08188 0.569287 4 12 ESTs, Weakly similar to OGT1_HUMAN UDP-N H78233 0.56928

20 11 12 UNK W90001 0.5692829 8 7 ESTs T78604 0.569289 2 11 ESTs, Moderately similar to ALU8_HUMAN A R00220 0.57024

13 14 10 Homo sapiens mRNA; cDNA DKFZp586N1323 (f R30941 0.5702432 8 9 L2DTL protein R06900 0.570244 10 5 diacylglycerol kinase, delta (130kD) AA280692 0.57128 4 3 U2(RNU2) small nuclear RNA auxillary fac W90506 0.5712

21 2 7 heparan sulfate 6-O-sulfotransferase W93511 0.572169 13 11 ESTs H50657 0.573128 11 2 fragile X mental retardation, autosomal N62761 0.57408

28 13 10 ESTs H60457 0.574082 1 6 KIAA0524 protein W32440 0.575049 5 11 ESTs H80519 0.57504

16 7 1 N-acylaminoacyl-peptide hydrolase AA455941 0.5750421 2 3 crystallin, zeta (quinone reductase) R40946 0.5750425 2 8 ESTs, Moderately similar to ALUC_HUMAN ! N68836 0.5750426 1 10 KIAA0554 protein H93605 0.5750428 13 8 ESTs T91110 0.5750429 6 6 creatine kinase, mitochondrial 2 (sarcom AA461048 0.5750417 9 3 PDZ domain protein (Drosophila inaD-like R98436 0.57624 12 7 ESTs, Highly similar to NOS3_HUMAN NITRI H01039 0.57626 3 11 plasminogen-like H59201 0.57628 12 11 ESTs H90908 0.5762 9 1 ubiquitin carrier protein AA464729 0.576967 3 10 ESTs H94849 0.57696

13 1 5 caspase 9, apoptosis-related cysteine pr AA281152 0.5769630 9 7 UNK T99192 0.5769612 4 3 parvalbumin AA010609 0.5779217 1 6 von Willebrand factor AA485883 0.5779221 10 1 tight junction protein 1 (zona occludens H50344 0.5779224 4 9 ESTs H38148 0.5779226 13 8 ESTs T97475 0.577929 14 6 UNK AA280931 0.57888

20 5 9 Homo sapiens NUF2R mRNA, complete cds R92435 0.578881 6 10 ESTs H58949 0.5808

17 10 2 A kinase (PRKA) anchor protein 6 R55786 0.580832 4 8 Homo sapiens clone 24411 mRNA sequence N75064 0.58081 8 9 UNK T98511 0.581762 14 9 ESTs R22035 0.58176


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4 10 10 PRO0195 protein N73761 0.581764 13 10 ESTs R88708 0.58176

10 2 10 KIAA1155 protein H94462 0.5817629 10 4 endothelial differentiation, sphingolipi R20666 0.5817630 9 3 homeo box D3 AA411169 0.5817631 12 3 CDC6 (cell division cycle 6, S. cerevisi H59204 0.5817616 13 11 ESTs H70622 0.5827221 3 10 ESTs R96844 0.582721 14 12 ESTs N74365 0.583682 4 8 ESTs R37495 0.583682 4 9 ESTs R91557 0.583684 6 13 EMPTY EMPTY 0.58368

12 4 11 lipoma HMGIC fusion partner N58145 0.5836817 1 10 UNK H53553 0.5836818 8 12 Ras-GTPase activating protein SH3 domain AA151214 0.5836828 5 6 UNK R22982 0.5836829 7 4 serine dehydratase T71363 0.583686 1 9 protease, serine, 23 R76394 0.58464

15 14 11 hypothetical protein DKFZp564D0462 R96212 0.5846420 2 12 hypothetical protein FLJ10466 AA453607 0.5846424 3 1 putative transmembrane protein AA454208 0.585620 2 11 sorting nexin 4 H73354 0.5865625 2 10 ESTs H53829 0.5865627 2 10 ESTs H54812 0.586562 6 12 ESTs, Weakly similar to ALU1_HUMAN ALU S AA055829 0.587527 4 6 complement component 4-binding protein, T62036 0.58752

26 12 9 MSTP032 protein W60846 0.5875232 6 7 chaperonin containing TCP1, subunit 4 (d T98634 0.587527 10 5 cysteine and glycine-rich protein 3 (car AA195959 0.588489 7 12 platelet-derived growth factor beta poly W68169 0.58848

17 2 9 EST R64580 0.5884826 11 6 likely ortholog of mouse polydom R33005 0.588486 7 3 UNK H80129 0.58944

16 4 9 ESTs H94263 0.589441 4 11 ESTs, Moderately similar to ALU7_HUMAN A R70361 | AI73 0.59043 5 12 CASP8 and FADD-like apoptosis regulator N94588 0.59048 13 4 thromboxane A2 receptor AA039932 0.59049 11 3 N-ethylmaleimide-sensitive factor attach AA010503 0.5904

25 4 6 retinol-binding protein 4, interstitial T72076 0.590426 7 10 Homo sapiens mRNA; cDNA DKFZp761D191 (fr N49273 0.590426 12 7 Homo sapiens ARTS protein (PNUTL2) mRNA, T64878 0.5913627 10 9 hypothetical protein FLJ20281 N54297 0.5913616 8 2 inositol 1,3,4-triphosphate 5/6 kinase AA463931 0.5923221 8 8 UNK T67528 0.5923225 4 9 ESTs R65963 0.592329 1 10 ESTs H53599 0.59328

13 2 7 ESTs, Weakly similar to ALU5_HUMAN ALU S R16109 0.5932814 1 7 KIAA1431 protein T80833 0.5932824 11 3 integrin, alpha 1 H68922 0.593284 14 11 EST H95044 0.594248 3 8 Homo sapiens cDNA: FLJ22918 fis, clone K H95071 0.59424

24 2 7 ESTs T85190 0.59424


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26 2 12 retinoic acid receptor responder (tazaro W47350 0.5942430 9 4 UNK R38383 0.594245 4 1 alpha thalassemia/mental retardation syn AA410435 0.5952

21 3 8 ESTs T97609 0.595224 9 5 caspase 8, apoptosis-related cysteine pr AA448468 0.595230 2 8 EST R11529 0.595221 3 3 complement component 5 N53664 0.5961631 8 2 nidogen 2 AA479199 0.596164 10 4 replication factor C (activator 1) 3 (38 H94617 0.59712

24 1 2 developmentally regulated GTP-binding pr AA456291 0.597125 7 4 keratin 19 AA464250 0.59808

16 2 5 ESTs AA284338 0.5980825 1 8 hypothetical protein PRO2521 N38810 0.598084 3 9 UNK R91394 0.599045 8 10 ESTs H56033 0.59904

13 13 12 EST N53758 0.5990420 13 1 reelin R45102 0.5990421 13 11 ESTs N74655 0.5990429 8 11 ESTs N70017 0.5990412 14 3 fucosidase, alpha-L- 1, tissue N95761 0.632 11 1 mitogen-activated protein kinase kinase H01340 0.623 14 5 myosin, light polypeptide 1, alkali; ske T52894 0.600966 1 2 retinoid X receptor, beta H42247 0.60192

10 3 1 keratin 13 W23757 0.6019224 3 2 paired box gene 8 AA405767 0.6019232 12 4 thyroid transcription factor 1 T60168 | AI82 0.6019221 6 6 corticotropin releasing hormone-binding AA287695 0.6028824 4 13 EMPTY EMPTY 0.6028830 12 8 ESTs N38818 0.602882 10 10 CEGP1 protein H93603 0.60384

20 13 12 UNK N68473 0.603841 10 9 ESTs R02562 0.60489 6 12 FYN oncogene related to SRC, FGR, YES N66144 0.6048

17 5 1 sarcoglycan, delta (35kD dystrophin-asso AA234982 0.604827 13 12 ESTs N74390 0.604829 6 7 ESTs H52098 0.604815 8 2 nucleosome assembly protein 1-like 4 H92201 0.6057627 13 10 membrane-bound transcription factor prot R54010 0.6057628 1 10 ESTs H95077 0.605762 13 2 toll-like receptor 3 R76099 0.606723 4 6 phorbolin (similar to apolipoprotein B m T64880 0.606729 5 12 MCF.2 cell line derived transforming seq N23406 0.60672

16 12 12 hypothetical protein MGC4276 similar to H69528 0.6067220 9 9 UNK R16557 0.6076825 14 9 ESTs R00766 0.6076832 9 3 serine/threonine kinase 9 N80713 0.6076820 14 4 protein tyrosine phosphatase, receptor t H18633 0.6086421 8 12 hypothetical protein FLJ14146 R25166 0.6086421 9 7 UNK N55563 0.60968 13 2 dystroglycan 1 (dystrophin-associated gl AA496691 0.61056

19 13 1 pim-1 oncogene AA447730 0.6105625 9 8 hypothetical protein FLJ20374 R52934 0.61056


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29 12 8 KIAA1020 protein T67442 0.6105612 8 12 UNK N49224 0.6115216 12 1 lectin, galactoside-binding, soluble, 7 AA011057 0.6115223 11 13 EMPTY EMPTY 0.6115230 7 8 UNK H69281 0.611522 7 12 karyopherin beta 2b, transportin R08897 0.612486 14 7 nucleoporin 155kD R21737 0.61248

30 14 4 ATP-binding cassette, sub-family B (MDR/ AA487429 0.6124828 14 4 protein tyrosine phosphatase, receptor t AA598513 0.6134432 14 8 ESTs, Moderately similar to ALU8_HUMAN A T81035 0.6134415 12 5 nuclear cap binding protein subunit 1, 8 AA278749 0.614417 5 9 mesenchymal stem cell protein DSC54 R63841 0.614425 5 1 selectin E (endothelial adhesion molecul H39991 0.614429 2 4 myxovirus (influenza) resistance 1, homo AA457042 0.614412 6 12 UNK R06284 0.6153621 4 7 ring finger protein 14 R06575 0.6153627 10 6 tumor necrosis factor receptor superfami AA453410 0.6153627 3 8 sirtuin (silent mating type information N55168 0.6153628 10 5 dihydrolipoamide branched chain transacy R89083 0.6153631 4 10 ESTs R98492 0.615363 9 3 potassium voltage-gated channel, KQT-lik W93500 0.616326 12 12 ESTs, Weakly similar to ELL2_HUMAN RNA P N57927 0.61632

19 7 11 ESTs AA016218 0.6163230 7 3 hepatic leukemia factor N70235 0.6163230 10 11 activating transcription factor 5 T90841 0.6163232 6 12 JAK binding protein AA485355 0.616323 2 1 KIAA0127 gene product AA489659 0.617285 7 9 uncoupling protein 4 H60586 0.61728

12 1 8 Homo sapiens cDNA FLJ13555 fis, clone PL R22926 0.6172814 2 8 ESTs R99220 0.6172817 6 5 UNK R91516 0.6172829 1 8 DKFZP434H132 protein T96944 0.6172830 10 10 ESTs, Weakly similar to ALU1_HUMAN ALU S R67601 0.617281 2 9 KIAA1546 protein R62583 0.61824

12 4 7 CGI-06 protein H82992 0.6182413 2 9 hypothetical protein FLJ11137 R63319 0.6182421 6 8 ESTs, Weakly similar to ALU1_HUMAN ALU S T66906 0.6182431 9 1 c-src tyrosine kinase AA079775 0.618245 5 1 visinin-like 1 H65066 0.6192

12 7 10 ESTs N52655 0.619216 13 10 ESTs H24580 | AI66 0.619225 3 3 annexin A7 H15446 0.61922 7 13 EMPTY EMPTY 0.620164 7 8 UNK R00045 0.62016

10 2 4 KIAA0077 protein AA403315 0.6201616 5 11 nuclear receptor subfamily 5, group A, m R10138 0.6201618 4 12 UNK W68559 0.6201619 8 13 EMPTY EMPTY 0.6201624 12 6 WW Domain-Containing Gene AA489498 0.6201624 9 12 Homo sapiens cDNA: FLJ22566 fis, clone H AA054715 0.620161 9 2 KIAA0322 protein R87213 0.62112

15 13 12 EST N55165 0.62112


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29 6 10 EST R98709 0.621122 4 1 interferon-induced protein with tetratri AA489640 0.622082 3 5 guanine nucleotide binding protein (G pr H92232 0.622083 3 6 glucose regulated protein, 58kD R33030 0.622085 4 7 chromosome 21 open reading frame 11 H43317 0.622089 4 12 UNK N32146 0.62208

10 3 4 KIAA0020 gene product AA454662 0.6220820 8 10 hypothetical protein PRO2822 N53043 0.6220816 14 13 Yamagata-TBD UNK 0.6230417 1 4 signal transducer and activator of trans AA280647 0.6230421 6 10 ESTs N66039 0.6230425 6 6 CD79A antigen (immunoglobulin-associated T86934 0.6230432 9 9 Homo sapiens cDNA FLJ20144 fis, clone CO H71813 0.623042 12 10 Homo sapiens cDNA: FLJ22256 fis, clone H H71857 0.6247 3 11 ESTs N64435 0.624

17 9 4 UNK T74567 0.62421 6 2 G protein-coupled receptor 37 (endotheli R66426 0.62430 12 11 ESTs H62842 0.6245 3 6 mutS (E. coli) homolog 2 (colon cancer, AA219061 0.624967 13 10 ESTs R31603 0.624969 3 9 UNK R62773 0.62496

10 3 6 ubiquitination factor E4B (homologous to W44725 0.6249612 12 11 UNK H91157 0.6249630 11 12 PCF11p homolog W73811 0.6249616 7 12 hypothetical protein FLJ20484 R06372 0.6259220 11 10 ESTs H47298 0.6259225 4 5 DEAD/H (Asp-Glu-Ala-Asp/His) box polypep AA485944 0.6259225 3 9 ESTs R62604 0.6259227 7 11 ESTs, Weakly similar to ALU5_HUMAN ALU S AA026756 0.625923 2 12 v-yes-1 Yamaguchi sarcoma viral related R83837 0.626886 3 11 ESTs H70121 0.62688

17 8 7 UNK H61046 0.6268824 10 11 ESTs R16656 0.626885 6 7 ESTs, Weakly similar to ALU1_HUMAN ALU S N73909 0.62784

17 1 1 KIAA0197 protein R43961 0.6278421 5 2 interleukin 18 receptor 1 AA482489 | AI 0.6278432 14 12 KIAA1681 protein T80943 0.627846 10 1 procollagen (type III) N-endopeptidase H98666 0.62886 9 2 PR domain containing 2, with ZNF domain W73060 0.62886 13 6 achaete-scute complex (Drosophila) homol AA441935 0.6288

13 13 4 DnaJ (Hsp40) homolog, subfamily B, membe AA455298 0.628820 5 7 DKFZP434J154 protein T67248 0.628827 2 9 ESTs R63206 0.628830 3 5 serine (or cysteine) proteinase inhibito T62086 0.628830 9 8 ESTs T80834 0.628831 7 5 suppression of tumorigenicity 14 (colon AA489246 0.62882 10 6 ubiquilin 1 N69460 0.62976

27 5 6 chloride intracellular channel 1 AA485913 0.6297629 5 1 wingless-type MMTV integration site fami W49672 0.6297629 8 10 ESTs R99004 0.6297619 11 10 EST R53900 0.6307228 4 7 hypothetical protein N73536 0.63072


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28 11 11 ESTs H90421 0.630722 12 13 EMPTY EMPTY 0.631685 8 8 DiGeorge syndrome critical region gene 8 T67557 0.631689 1 7 Homo sapiens, clone IMAGE:3508146, mRNA, T97694 0.631689 3 7 adaptor-related protein complex 1, gamma N78621 0.63168

14 11 6 hypothetical protein FLJ20037 R69088 0.6316816 1 10 ESTs N55264 0.6316819 13 2 ESTs, Highly similar to PA2X_HUMAN CALCI T94293 0.6316830 7 7 beta tubulin 1, class VI N64431 0.631681 7 13 EMPTY EMPTY 0.632645 4 8 ESTs T97764 0.63264

21 1 3 cyclin-dependent kinase 6 H73724 0.6326413 11 11 Homo sapiens, clone MGC:12422, mRNA, com N63753 0.633627 9 9 ESTs T99071 0.633627 12 11 ESTs N74942 0.63366 1 12 phospholipase C, beta 2 AA464970 0.63456

29 7 10 ESTs H65942 0.6345631 3 1 villin 2 (ezrin) AA411440 0.6345631 7 3 homeo box D9 AA424871 0.634566 8 12 ESTs, Weakly similar to WDNM RAT WDNM1 P AA149250 0.635527 8 1 transcription factor 7 (T-cell specific, AA481988 0.63552

10 5 1 intercellular adhesion molecule 3 AA479188 0.6355223 11 2 zinc finger protein AA034215 0.6355230 7 4 KIAA0268 protein AA429602 0.635527 1 2 nuclear matrix protein p84 AA129297 0.63648

13 8 9 ESTs T97004 0.6364820 9 10 ESTs H73202 0.6364824 13 7 anaphase-promoting complex 1; meiotic ch N68492 0.636484 10 13 EMPTY EMPTY 0.63744

15 13 1 solute carrier family 9 (sodium/hydrogen AA455369 0.6374427 8 11 HSPC049 protein R14977 0.6374412 11 10 ESTs H47335 0.638417 13 12 ESTs N74055 0.638420 10 11 Homo sapiens cDNA FLJ12292 fis, clone MA N50948 0.638421 14 7 Homo sapiens mRNA for alanine-glyoxylate R83191 0.638422 4 7 ESTs, Highly similar to Gene product wit T70337 0.638429 5 8 ESTs T66825 0.638415 12 11 ESTs R93696 0.6393631 1 11 ESTs N55457 0.6393611 13 2 GCN1 (general control of amino-acid synt R55251 0.6403216 8 8 ESTs T77813 0.6403221 2 5 hypothetical protein FLJ22624 AA489007 0.6403224 3 9 ESTs N58513 0.6403230 6 4 guanine nucleotide exchange factor for R AA451891 0.6403224 10 7 sema domain, immunoglobulin domain (Ig), R33439 0.642241 1 9 ESTs R62137 0.64322 14 1 seven in absentia (Drosophila) homolog 1 AA455956 0.6432

13 1 11 ESTs N54911 0.643213 1 12 S-adenosylmethionine decarboxylase 1 AA504772 0.643228 9 12 NDRG family, member 4 AA454215 0.64323 7 9 ESTs, Moderately similar to ALU7_HUMAN A T98842 0.644164 6 3 cholecystokinin N30191 0.64416


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7 3 4 fatty-acid-Coenzyme A ligase, long-chain T73556 0.6441621 1 6 dCMP deaminase AA448207 0.6441622 6 11 chordin-like AA040425 0.6441625 11 12 EST H77737 0.6441629 5 3 UNK R09220 0.6441629 3 4 major histocompatibility complex, class AA486532 0.6441630 14 8 ESTs T86714 0.644163 10 1 UNK R90744 0.645123 4 12 UNK N63635 0.645125 6 1 UDP glycosyltransferase 2 family, polype H68509 0.645128 3 6 frizzled (Drosophila) homolog 6 H71941 0.64512

10 5 9 ESTs R91798 0.6451216 3 5 transmembrane protein (63kD), endoplasmi AA598787 0.6451224 4 3 serum response factor (c-fos serum respo AA487973 0.6451224 1 6 hypothetical protein FLJ23306 T58873 0.6451228 4 8 KIAA0720 protein R34122 0.6451230 4 3 spleen tyrosine kinase AA598572 0.6451230 14 6 galactose-4-epimerase, UDP- AA280832 0.645127 8 3 transmembrane protein 1 N65981 0.64608

20 12 7 ring finger protein 21, interferon-respo H50872 0.6460829 2 7 KIAA0561 protein H54286 0.6460815 12 1 spermidine/spermine N1-acetyltransferase AA011215 0.6470425 1 3 apolipoprotein C-III N53169 0.6470427 9 10 pregnancy-associated plasma protein A R67000 0.6470428 10 10 UNK R98739 0.647041 9 11 transforming, acidic coiled-coil contain H47327 0.6484 7 11 adenylate cyclase 1 (brain) W69791 0.6487 5 9 Homo sapiens cDNA FLJ20848 fis, clone AD R77213 | R772 0.648

20 10 1 DnaJ (Hsp40) homolog, subfamily C, membe H09811 0.64821 6 9 ESTs R34837 0.64824 10 8 ESTs, Moderately similar to ALU1_HUMAN A H90508 0.64826 12 8 ESTs R01426 0.64832 6 9 ESTs N75967 0.64812 14 10 ESTs R88771 0.6489614 5 4 KIAA0377 gene product H29592 0.6489625 3 5 transcription factor Dp-2 (E2F dimerizat AA465369 0.6489628 11 12 hypothetical protein FLJ21959 AA443125 0.648961 8 7 UNK N62539 0.64992

10 5 3 phospholamban AA427940 0.6499213 9 4 endothelin receptor type B H28710 0.6499217 6 10 ESTs H58814 0.6499224 7 8 EST T71541 0.649924 14 9 ESTs, Highly similar to C57785 zinc fing R22450 0.65088

12 14 8 ESTs T91040 0.6508813 10 10 alpha-methylacyl-CoA racemase R25375 0.6508825 5 6 major histocompatibility complex, class T63324 0.6508826 9 7 ESTs AA027950 0.650884 5 9 LIM protein (similar to rat protein kina R92455 0.651848 10 12 ESTs N23753 0.651849 4 11 KIAA0669 gene product R62374 0.65184

12 5 9 ESTs R92453 0.6518418 3 1 kinase insert domain receptor (a type II AA027012 0.65184


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3 3 5 peroxisome biogenesis factor 1 AA598527 0.652823 14 3 26S proteasome-associated pad1 homolog R08876 0.652825 6 3 acyl-Coenzyme A dehydrogenase, short/bra H95792 0.652827 7 5 filamin B, beta (actin-binding protein-2 AA486239 0.65287 2 1 RNA-binding protein gene with multiple s W67200 0.65376

30 11 6 ESTs, Weakly similar to KIAA0349 [H.sapi R99331 0.6537632 6 6 Homo sapiens cDNA: FLJ23404 fis, clone H N57658 0.653765 5 8 ESTs T66849 0.65472

20 3 3 KIAA0220 protein AA449463 0.6547231 6 3 protein phosphatase 3 (formerly 2B), cat N89721 0.654721 8 4 plectin 1, intermediate filament binding AA448400 0.655683 1 11 homeo box C4 N80567 0.656644 10 12 UNK H25846 | AI66 0.65664

28 7 10 ESTs, Weakly similar to ALU5_HUMAN ALU S N52535 0.6566410 2 3 protein kinase C substrate 80K-H AA496810 0.657617 9 2 KIAA0335 gene product AA410363 0.657617 7 6 erythrocyte membrane protein band 4.1-li R71689 0.657631 11 2 melan-A N26562 0.65762 6 11 ESTs N65995 0.658563 8 2 spectrin, beta, non-erythrocytic 2 H28127 0.65856

16 5 8 ESTs T90290 0.6585623 11 12 hypothetical protein FLJ22242 R71481 0.6585613 2 2 UNK W02116 0.6595216 4 7 ESTs, Moderately similar to S35305 zinc W31465 0.6595223 12 10 uncharacterized hypothalamus protein HT0 R31544 0.6595229 4 7 EST, Moderately similar to DEHUA6 alcoho T87343 0.659521 6 8 ESTs, Moderately similar to ALU1_HUMAN A R26798 0.660482 11 11 multiple PDZ domain protein AA029940 0.660482 4 12 hypothetical protein FLJ10024 AA147928 0.660488 4 7 hypothetical protein FLJ12985 N71457 0.66048

10 4 10 tubulin, beta polypeptide N33934 0.6604820 8 12 cisplatin resistance related protein CRR H84444 0.6604828 5 11 EST N73588 0.660484 2 10 Homo sapiens cDNA FLJ14162 fis, clone NT N55012 0.66144

19 14 1 phosphoenolpyruvate carboxykinase 1 (sol AA405731 0.6614429 3 10 ESTs N66843 0.6614431 4 11 ESTs N64696 0.661449 9 7 ESTs N68424 0.6624

28 12 8 ESTs T91198 0.66241 5 13 EMPTY EMPTY 0.663368 11 12 DKFZP564A122 protein N31577 0.663369 3 11 ESTs, Moderately similar to ALU7_HUMAN A R12492 0.66336

10 9 4 S100 calcium-binding protein, beta (neur AA424045 0.6633611 13 11 UNK H53045 0.6633615 5 2 dual specificity phosphatase 5 W65461 0.6633624 6 4 amyloid beta precursor protein (cytoplas AA046411 0.663365 2 1 kinesin-like 2 N69491 0.66432

20 14 6 dynein, cytoplasmic, light intermediate AA454959 0.664323 2 2 histidine-rich glycoprotein H70473 0.665283 6 5 thyroid hormone receptor interactor 11 AA481028 0.66528

17 13 11 CASP8 associated protein 2 H50583 0.6652825 10 1 pyruvate kinase, liver and RBC R08829 0.66528


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29 9 7 UNK H52361 0.6652831 3 5 protein tyrosine kinase 2 beta R85257 0.6652812 8 3 growth associated protein 43 H05445 0.6662412 12 10 UNK R86772 0.666243 7 3 potassium voltage-gated channel, KQT-lik H51419 0.6672

12 12 5 Nef-associated factor 1 T64483 0.667217 5 10 EST H56894 0.667221 6 4 low density lipoprotein receptor (famili AA504461 0.667229 11 7 src homology three (SH3) and cysteine ri AA031284 0.667217 6 4 Human proteinase activated receptor-2 mR AA454652 0.6681618 8 2 carboxylesterase 2 (intestine, liver) AA099487 0.6681629 5 6 cyclin D1 (PRAD1: parathyroid adenomatos AA487486 0.6681610 8 4 calcium/calmodulin-dependent protein kin H29322 0.6691223 13 12 EST N55085 0.6691225 10 4 cerebellin 1 precursor AA425008 0.669127 7 5 IQ motif containing GTPase activating pr W32272 0.67008

24 11 11 ESTs, Weakly similar to ALU1_HUMAN ALU S H64938 0.670085 2 4 Homo sapiens, clone MGC:12401, mRNA, com AA196393 0.671048 13 5 neuroblastoma, suppression of tumorigeni AA598830 0.67104

17 5 4 phosphodiesterase 4D, cAMP-specific (dun AA481397 0.6710420 6 12 UNK N26072 0.6710432 13 5 KIAA0864 protein AA497027 0.671042 5 3 phosphogluconate dehydrogenase AA598759 0.6729 8 1 replication protein A2 (32kD) R39861 0.6729 1 6 v-yes-1 Yamaguchi sarcoma viral related R83837 0.672

19 12 11 ESTs H54253 0.67224 12 11 ESTs H65335 0.67227 14 1 EST H22563 0.6722 8 12 protein kinase D2 AA428451 0.672965 12 8 ESTs, Moderately similar to ALU6_HUMAN A H98701 0.67296

20 1 9 ESTs R89824 0.6729620 4 10 ESTs, Weakly similar to A47582 B-cell gr R19406 0.6729630 4 12 UNK R78554 | R785 0.672963 1 7 Homo sapiens cDNA: FLJ23388 fis, clone H N39168 0.673928 14 2 Human clone 23932 mRNA sequence R39464 0.673928 3 13 EMPTY EMPTY 0.67392

10 1 6 ESTs R95132 0.6739220 1 6 ESTs N80149 0.6739224 10 4 v-erb-b2 avian erythroblastic leukemia v AA446928 0.674884 5 7 ESTs W67951 0.675847 5 10 EST R98687 0.67584

27 9 7 ESTs, Moderately similar to transmembran R02580 0.6758428 13 9 ESTs R22189 0.675849 6 11 EST N68820 0.6768

22 1 4 S-antigen; retina and pineal gland (arre AA057233 0.676827 1 9 ESTs R62926 0.67688 2 12 ESTs, Highly similar to hypothetical pro AA004878 0.67776

15 7 5 HIV-1 rev binding protein 2 W52273 0.6777619 3 1 transgelin 2 H08564 0.6777627 10 10 ESTs, Highly similar to immunoglobulin k AA028884 0.6777628 9 1 UNK H77652 0.6777632 3 12 secreted protein of unknown function AA156251 0.67776


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1 7 7 ESTs T95064 0.678723 4 10 Homo sapiens cDNA: FLJ22063 fis, clone H H77736 0.67872

16 13 2 elastin (supravalvular aortic stenosis, AA459308 0.6787221 3 11 ESTs N58409 0.6787225 13 9 ESTs R92577 0.6787226 1 9 ESTs R89594 0.678722 4 3 phosphoribosylglycinamide formyltransfer AA598487 0.679683 8 1 RAB5A, member RAS oncogene family H11455 0.679689 9 6 adenosine deaminase, RNA-specific, B1 (h AA489331 0.67968

25 12 7 ESTs N66852 0.6796826 5 12 ESTs N72009 0.679688 7 1 N-acetylglucosaminidase, alpha- (Sanfili N77931 0.68064

13 7 1 cell division cycle 34 H20743 0.6806416 1 2 phosphatidylinositol glycan, class K N70544 0.680641 2 2 chromodomain helicase DNA binding protei N49703 0.68161 7 8 BTB and CNC homology 1, basic leucine zi N74648 0.6816

16 6 8 hypothetical protein FLJ11715 T71133 0.681617 4 2 FKBP-associated protein N66028 0.681621 11 9 Homo sapiens, Similar to KIAA0626 gene p R02095 0.681624 12 9 adenylate cyclase 9 H64281 0.681630 14 2 dystrobrevin, alpha H09172 0.681617 11 10 dishevelled 2 (homologous to Drosophila R38325 0.682561 9 8 ESTs N34056 0.68352

13 5 3 Rho GTPase activating protein 1 AA427934 0.6844817 12 7 distal-less homeo box 5 N74882 0.6844830 2 9 hypothetical protein H99695 0.6844831 1 10 acyl-Coenzyme A oxidase 1, palmitoyl H72834 0.6844827 10 8 likely homolog of rat kinase D-interacti T95100 0.6854413 2 11 EST N58281 0.686415 13 8 androgen induced protein T67558 0.686415 13 11 EST R94601 0.686420 1 10 ESTs H95086 0.686424 9 7 hypothetical protein FLJ10955 H57727 0.686432 13 10 KIAA1617 protein R72879 0.68643 3 12 UNK H75531 0.687366 14 13 PRKase gene Cont-10 0.687367 3 8 ESTs T98008 0.68736

16 9 3 translin-associated factor X AA477514 0.6873617 10 10 ESTs, Weakly similar to ALU7_HUMAN ALU S R36624 0.6873628 9 9 KIAA1272 protein H59079 0.6873628 12 12 Homo sapiens clone PP1226 unknown mRNA AA464739 0.6873630 12 9 UNK R07650 0.6873632 10 9 ESTs R08110 0.6873628 9 10 ESTs, Weakly similar to ALU8_HUMAN ALU S R84407 0.6883230 4 9 ESTs H43657 | AI82 0.6883232 8 10 hypothetical protein DKFZp762M115 H80724 0.688327 8 4 Homo sapiens cDNA FLJ11904 fis, clone HE AA447098 0.689287 4 10 EST R98583 0.68928

15 2 7 insulin induced protein 2 T91091 0.6892815 14 8 chromosome 1 open reading frame 12 H56028 0.6892817 12 9 ESTs, Weakly similar to ALU6_HUMAN ALU S T99834 0.6892818 6 9 ESTs R92032 0.68928


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26 9 11 Homo sapiens cDNA FLJ20767 fis, clone CO R31679 0.6892830 2 2 cadherin 1, type 1, E-cadherin (epitheli H97778 0.6892820 3 9 ESTs R91271 0.6902421 1 2 SWI/SNF related, matrix associated, acti AA459407 0.6902421 3 9 hypothetical protein FLJ12541 similar to R32754 0.6902421 4 11 EST N59792 0.6902424 7 2 UNK N52293 0.6902425 11 8 ESTs R16120 0.6902430 11 1 met proto-oncogene (hepatocyte growth fa AA411642 0.6902432 5 10 UNK H73909 0.690243 12 11 ESTs H52702 0.6912

12 1 12 E1B-55kDa-associated protein 5 AA464198 0.691216 3 9 UNK H25907 | AI66 0.691224 5 10 phospholysine phosphohistidine inorganic H74009 0.691227 3 2 hippocalcin H18971 0.691227 12 12 Homo sapiens mRNA; cDNA DKFZp434A2410 (f R52161 | AI73 0.69125 10 4 mitogen-activated protein kinase 3 AA454819 0.69216

16 14 5 mesothelin AA488406 0.6921616 8 7 ESTs, Weakly similar to ribosomal protei H67666 0.6921621 13 4 mitogen-activated protein kinase kinase R44740 0.6921624 4 7 Homo sapiens cDNA FLJ13496 fis, clone PL R78627 0.6921632 12 11 ESTs H65044 0.692163 4 8 ESTs H22137 0.693127 12 12 PRO1659 protein N64781 0.69312

28 10 1 Homo sapiens cDNA: FLJ23539 fis, clone L R01238 0.6931229 11 11 ESTs R97106 0.693123 9 4 CD36 antigen (collagen type I receptor, N39161 0.69408

11 11 1 protein kinase C, beta 1 AA479102 0.6940813 10 9 ESTs, Moderately similar to ALU7_HUMAN A T95128 0.6940824 3 8 KIAA1449 protein R19414 0.6940829 13 9 UNK T96601 0.6940828 11 2 TNF receptor-associated factor 6 AA456295 0.6950429 10 10 EST R27329 0.6950430 10 2 HLA class II region expressed gene KE4 AA146655 0.6950430 3 3 transducin-like enhancer of split 1, hom T61445 0.695043 3 10 ESTs H57273 0.696

12 3 1 integrin, alpha L (antigen CD11A (p180), R48796 0.69612 9 10 ESTs N28270 0.69629 11 1 thrombomodulin H59861 0.6963 4 7 ESTs, Weakly similar to ALU4_HUMAN ALU S N54805 0.696965 6 11 ESTs N68456 0.69696

13 1 6 damage-specific DNA binding protein 1 (1 AA608557 0.6969624 9 8 ESTs R98997 0.6969629 9 3 ELAV (embryonic lethal, abnormal vision, W72322 0.6969631 3 3 coagulation factor II (thrombin) recepto AA455910 0.6969629 1 2 mitogen-activating protein kinase kinase R50953 0.6979232 14 11 ESTs H68313 0.6979210 3 5 guanine nucleotide binding protein (G pr AA487912 0.6988818 7 2 cleavage and polyadenylation specific fa N92864 0.6988823 13 11 ESTs R94212 0.6988830 13 3 bleomycin hydrolase AA417881 0.6988831 2 8 ESTs T97780 0.69888


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9 2 5 protein with polyglutamine repeat; calci AA481554 0.6998416 2 11 growth hormone receptor N70358 0.6998416 14 11 ESTs H68621 0.6998417 10 12 UNK R93009 0.6998428 11 3 glucosamine (N-acetyl)-6-sulfatase (Sanf AA035347 0.6998428 1 11 ESTs N69574 0.6998423 14 11 ESTs, Weakly similar to cell fate specif R96208 0.700830 1 7 bridging integrator 2 R12369 0.70081 12 4 breakpoint cluster region AA419342 0.701762 9 8 Homo sapiens mRNA; cDNA DKFZp564H1916 (f N55339 0.70176

12 10 7 UNK N74084 0.7017619 9 12 EST H12777 0.701761 3 13 EMPTY EMPTY 0.702722 3 11 ESTs H66856 0.70272

15 7 8 ESTs T67456 0.7027216 14 8 ESTs, Moderately similar to ALU1_HUMAN A T99780 0.7036821 1 5 cytoskeleton-associated protein 1 AA504477 0.7036828 13 11 ecotropic viral integration site 5 H91310 0.703683 10 9 ESTs H65490 0.704647 3 5 butyrophilin, subfamily 3, member A3 AA478585 0.70464

24 6 7 UNK H82068 0.7046424 6 13 EMPTY EMPTY 0.7046429 8 12 perilipin T70586 0.7046432 13 8 kidney- and liver-specific gene N58170 0.704642 13 11 ESTs H91265 0.70563 9 2 cadherin, EGF LAG seven-pass G-type rece H39187 0.7056

26 5 9 ESTs R92176 0.70562 9 12 ninein (GSK3B interacting protein) N34687 0.706565 6 10 ESTs R98774 0.706566 13 2 gamma-glutamyl hydrolase (conjugase, fol AA456621 0.706567 14 9 ESTs T97301 0.706569 11 10 hypothetical protein FLJ21044 similar to N80769 0.70656

14 14 5 src homology three (SH3) and cysteine ri AA031284 0.7065624 1 10 RAB36, member RAS oncogene family H69004 0.706563 5 7 binder of Arl Two T64938 0.70752

14 5 12 Homo sapiens cDNA FLJ13443 fis, clone PL W51832 | AA2 0.7075216 9 8 EST T77785 0.7075216 5 10 ESTs H74330 0.7075224 2 11 ESTs, Moderately similar to unknown [H.s R68669 0.707526 3 12 ESTs, Weakly similar to T15138 hypotheti AA464689 0.708488 6 5 A kinase (PRKA) anchor protein 1 AA454947 0.70848

12 6 11 ESTs, Weakly similar to ALU6_HUMAN ALU S T97257 0.7084816 10 12 Homo sapiens cDNA FLJ12263 fis, clone MA W86660 0.7084827 12 10 ornithine decarboxylase antizyme 1 T97702 0.7084829 6 11 UNK N68171 0.7084829 4 8 ESTs T97707 0.7094416 10 6 hypothetical protein FLJ20980 N53492 0.710417 9 9 ESTs T98531 0.710421 13 6 glycoprotein M6A AA447632 0.710424 8 2 Human insulin-like growth factor binding H08561 0.711368 5 4 upstream regulatory element binding prot AA446600 0.71232

13 4 13 EMPTY EMPTY 0.71232


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25 1 4 pleckstrin homology, Sec7 and coiled/coi AA490903 0.712323 11 6 histidyl-tRNA synthetase H61209 0.713286 11 8 ESTs H95238 0.713287 2 11 ESTs N62567 0.713282 2 9 ESTs R89285 0.714242 6 13 EMPTY EMPTY 0.714249 7 3 mitochondrial ribosomal protein S12 R23752 0.714249 10 7 protein predicted by clone 23627 W95389 0.71424

13 13 9 ESTs T96665 0.7142416 7 7 vacuolar sorting protein 4 W74136 0.7142420 3 10 ESTs, Weakly similar to ALU8_HUMAN ALU S N33620 0.7142425 4 10 ESTs H56424 0.7142429 1 10 ESTs R94923 0.714242 14 7 DKFZP564A122 protein AA025807 0.71522 5 10 ESTs N73563 0.7152

30 7 10 ESTs H78083 0.715232 10 2 H2B histone family, member Q AA456298 0.715229 4 4 MADS box transcription enhancer factor 2 AA283744 0.716163 10 10 UNK R53815 0.717128 8 6 myelin gene expression factor 2 H96671 0.71712

13 1 9 Homo sapiens mRNA for partial 3'UTR, seq R31946 0.7171216 11 6 ESTs, Weakly similar to Z228_HUMAN ZINC H93450 0.717127 11 12 Homo sapiens cDNA FLJ12900 fis, clone NT H68664 0.718089 12 5 immunoglobulin heavy constant mu H73590 0.71808

15 8 11 ESTs, Weakly similar to putative integra N71631 0.7180829 11 10 ESTs R37816 0.7180832 3 8 ESTs R19411 0.718084 13 7 TASP for testis-specific adriamycin sens T64972 0.71904

22 1 10 ESTs, Weakly similar to ALU4_HUMAN ALU S H66741 0.7190431 8 7 EST T90201 0.719044 6 10 ESTs N52279 0.728 4 1 UNK R70868 0.728 13 11 UNK H70641 0.72

12 3 11 ESTs N55108 0.7230 1 3 tyrosine kinase with immunoglobulin and AA432062 0.7231 8 4 interferon, alpha-inducible protein (clo AA448478 0.720962 13 6 hydroxysteroid (17-beta) dehydrogenase 4 AA487914 0.72192

11 13 5 Human transposon-like element mRNA AA496800 0.7219212 5 11 serologically defined colon cancer antig N74362 0.7219221 12 6 coagulation factor X N98524 0.7219225 5 10 ESTs H95358 0.7219225 7 12 epidermal growth factor receptor (avian W48713 0.721921 10 10 ESTs R37814 0.72288

17 11 3 inhibitor of growth 1 family, member 1 N33574 0.7228832 4 10 Rag D protein N54401 0.722882 7 9 hypothetical protein from clone 24828 H50505 0.723843 1 3 cytochrome b5 outer mitochondrial membra AA406485 0.723845 11 11 ESTs N58363 0.72384

20 7 8 ESTs T90369 0.7238420 10 12 Homo sapiens, Similar to RIKEN cDNA 2810 N95217 0.7238424 6 8 Homo sapiens cDNA FLJ11302 fis, clone PL T70612 0.723847 5 8 EST T67136 0.7248


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10 7 1 caspase 1, apoptosis-related cysteine pr T95052 0.724820 13 10 ESTs R87194 0.724832 13 2 Kruppel-like factor 1 (erythroid) H65734 0.72481 4 12 baculoviral IAP repeat-containing 1 AA621150 0.72576

17 1 12 FOS-like antigen-1 T89996 0.7257617 6 12 ras homolog gene family, member B N41062 0.7257622 9 7 UNK R93354 0.7257625 6 10 Homo sapiens cDNA FLJ12776 fis, clone NT N64033 0.725762 2 12 adaptor-related protein complex 1, gamma AA464255 0.726723 1 5 guanine nucleotide binding protein 10 AA460286 0.726727 6 6 coagulation factor VIII, procoagulant co AA426469 0.726729 2 12 wingless-type MMTV integration site fami H04382 0.72672

14 14 7 TIA1 cytotoxic granule-associated RNA-bi R33841 0.726725 4 2 claudin 10 AA457739 0.727685 6 12 ESTs H79253 0.727686 7 8 ESTs T80117 0.72768

13 13 3 tumor protein p53-binding protein, 2 H69077 0.7276814 4 4 tousled-like kinase 2 AA599008 0.727681 2 8 sirtuin (silent mating type information N53491 0.728647 7 6 gap junction protein, alpha 1, 43kD (con AA487623 0.72864

19 1 10 ESTs H54720 0.7286420 13 4 proteoglycan 1, secretory granule AA278759 0.7286413 8 10 hypothetical protein R99084 0.729621 4 10 ESTs, Weakly similar to Pro-Pol-dUTPase N54993 0.729626 11 8 Homo sapiens mRNA for HMG-box transcript T91787 0.729630 13 6 nucleolar GTPase AA446557 0.729632 14 1 Fc fragment of IgG, low affinity IIIb, r H20822 0.729632 14 6 numb (Drosophila) homolog AA487149 0.729632 5 11 Homo sapiens cDNA: FLJ20944 fis, clone A N73575 0.729621 9 11 ESTs N70382 0.7305628 6 8 synaptosomal-associated protein, 23kD R98966 0.7305625 13 1 prostaglandin E receptor 3 (subtype EP3) AA406362 0.7315228 1 13 EMPTY EMPTY 0.731525 12 1 TIA1 cytotoxic granule-associated RNA-bi N59426 0.732485 7 10 ESTs R98957 0.73248

26 5 11 hypothetical protein FLJ23053 R25654 0.732481 8 8 cat eye syndrome chromosome region, cand R98055 0.73344

24 6 10 UNK H79613 0.7334425 10 7 VNN3 protein T95504 0.7334432 3 7 biglycan N51018 0.733441 7 10 ESTs H59559 0.73441 10 11 hypothetical protein DKFZp434L0117 T71568 0.73444 8 13 EMPTY EMPTY 0.73445 3 2 splicing factor (CC1.3) H47069 0.7344

14 4 2 ESTs H45967 0.734416 3 10 ESTs H73305 0.734421 8 1 small nuclear ribonucleoprotein 70kD pol R02346 0.734425 4 8 EST R02357 0.734427 11 7 ESTs, Highly similar to I38945 melanoma N51475 0.734430 9 1 myelin basic protein H17080 0.73441 4 13 EMPTY EMPTY 0.735362 3 2 fibrillin 1 (Marfan syndrome) AA418674 0.73536


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28 6 12 ESTs, Weakly similar to ALU1_HUMAN ALU S N32155 0.7353629 4 5 Human clone 23574 mRNA sequence AA113331 0.7353629 4 10 ESTs R97234 0.7353631 7 11 ESTs N69656 0.735364 13 11 ESTs N59413 0.73632

12 1 9 ESTs H66650 0.7363214 13 6 bridging integrator 1 AA453175 0.7363224 6 9 ESTs R83017 0.736321 4 3 adrenergic, beta, receptor kinase 1 R88247 0.737286 11 5 calcium channel, voltage-dependent, L ty H29256 0.737288 9 1 matrix metalloproteinase 1 (interstitial AA143331 0.737289 13 9 ESTs, Weakly similar to ALU7_HUMAN ALU S R00545 0.73728

10 12 3 UNK AA058857 0.7372820 4 2 epoxide hydrolase 2, cytoplasmic R73525 0.7372831 4 1 arginine vasopressin receptor 1A AA448190 0.737287 7 8 hypothetical protein FLJ20307 T67479 0.73824

16 9 11 EST, Weakly similar to ALUB_HUMAN !!!! A R07618 0.7382417 5 5 FK506-binding protein 5 W86653 0.7382418 2 11 ESTs H52198 0.738245 9 5 hyaluronoglucosaminidase 2 AA453401 0.7392

23 12 3 phospholipase A2, group V R32410 0.739224 3 4 KIAA0355 gene product AA432052 0.739229 9 9 ESTs R06363 0.739230 10 8 low density lipoprotein receptor-related N71497 0.73922 5 12 ESTs N24645 0.740163 6 10 EST H60523 0.74016

26 8 8 ESTs T90541 0.7401628 6 11 ephrin-B2 AA461108 0.7401630 12 7 DEAD/H (Asp-Glu-Ala-Asp/His) box polypep T70346 0.7401621 6 7 ESTs, Weakly similar to ALUB_HUMAN !!!! H93086 0.7411224 4 10 ring finger protein 3 N66000 0.7411226 14 3 glutamate-cysteine ligase, catalytic sub H56069 0.7411217 11 5 insulin-like growth factor 2 receptor T62547 0.7420817 3 11 Homo sapiens cDNA: FLJ21691 fis, clone C R01176 0.7420817 11 12 UNK N66845 0.7420826 12 2 ilvB (bacterial acetolactate synthase)-l AA451741 0.7420827 13 3 KIAA0423 protein N49914 0.7420832 3 13 EMPTY EMPTY 0.742083 13 1 phospholipase C, gamma 2 (phosphatidylin H57180 0.743047 2 8 EST T97931 0.74304

13 3 4 macrophage stimulating 1 (hepatocyte gro T47813 0.7430425 6 11 ESTs N39018 0.7430427 13 2 KIAA0222 gene product W58090 0.743044 11 12 ESTs N24786 0.744

21 3 7 ESTs N92697 0.7443 4 11 Homo sapiens, clone IMAGE:3355383, mRNA, N71545 0.744967 6 10 ESTs R99277 0.74496

17 11 8 ESTs R27299 0.7449621 5 3 centromere protein E (312kD) AA411850 0.744963 3 2 UNK H71868 0.745926 7 1 translocated promoter region (to activat R11490 0.745926 2 2 ClpP (caseinolytic protease, ATP-depende W58658 0.74592


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8 13 1 lymphotoxin beta receptor (TNFR superfam AA454646 0.7459211 6 8 ESTs R44999 0.7459220 9 13 EMPTY EMPTY 0.7459229 5 10 ESTs R97986 0.745923 6 4 37 kDa leucine-rich repeat (LRR) protein AA423870 0.746888 5 3 signal transducer and activator of trans AA488075 0.746889 10 8 hypothetical protein FLJ20507 H53733 0.746883 7 8 ESTs, Weakly similar to endo-alpha-D-man N91900 0.747848 9 11 EST R12474 0.74784

16 4 10 ESTs N66063 0.7478424 7 10 UNK H80336 0.7478427 7 10 ESTs H82846 0.747845 5 10 ESTs R98101 0.7488

13 12 9 ESTs T95596 0.748831 8 11 hypothetical protein FLJ20701 N71484 0.74883 2 11 hypothetical protein FLJ23024 R91167 0.74976

10 8 1 inter-alpha (globulin) inhibitor, H2 pol R06580 0.7497620 12 8 prenylcysteine lyase R78527 | R784 0.7497610 3 9 UNK R89471 0.7507218 8 7 NADH dehydrogenase (ubiquinone) Fe-S pro T96621 0.7507232 8 12 ESTs, Weakly similar to T46460 hypotheti T87132 0.750722 4 10 hypothetical protein FLJ12890 N62475 0.75168

28 13 2 T54 protein R37743 0.7516831 7 9 DKFZP434D1335 protein H25229 0.751681 3 2 actin related protein 2/3 complex, subun W55964 0.752646 13 5 mitogen-activated protein kinase kinase AA150828 0.752647 10 3 Human D9 splice variant B mRNA, complete AA453750 0.752648 9 5 M-phase phosphoprotein 10 (U3 small nucl AA504113 0.75264

24 7 1 diaphorase (NADH/NADPH) (cytochrome b-5 AA458634 0.7526425 8 10 ESTs H59602 0.7526426 7 12 gene with multiple splice variants near AA150741 0.7526432 8 13 EMPTY EMPTY 0.7526420 6 7 Homo sapiens mRNA full length insert cDN W20487 0.753629 1 7 ESTs N53076 0.75364 7 9 ESTs R16784 0.754565 10 2 p300/CBP-associated factor N74637 0.75456

10 13 8 tousled-like kinase 2 H60709 0.7545612 1 4 KIAA0040 gene product AA465479 0.7545617 1 9 hepatocellular carcinoma-associated anti W96149 0.7545623 12 12 filamin B, beta (actin-binding protein-2 H78098 0.754563 6 7 Homo sapiens cDNA: FLJ21887 fis, clone H H73337 0.755525 9 4 endothelin 3 T67004 0.75552

17 11 11 formiminotransferase cyclodeaminase N74650 0.7555219 12 2 KIAA0231 protein N63991 0.7555228 12 4 pyruvate dehydrogenase (lipoamide) alpha T65758 0.7555230 6 10 ESTs H75860 0.755522 12 3 glucuronidase, beta N34827 0.75648

12 7 9 hypothetical protein FLJ21945 R16779 0.7564814 1 10 BANP homolog, SMAR1 homolog N68408 0.7564815 4 3 carbonic anhydrase II H23187 0.7564816 6 2 Rac/Cdc42 guanine exchange factor (GEF) AA236957 0.7564820 2 3 programmed cell death 2 AA521466 0.75648


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31 14 9 Homo sapiens, clone MGC:10871, mRNA, com N54042 0.756481 11 3 folylpolyglutamate synthase R44864 0.757445 5 9 hypothetical protein R34682 0.75744

12 2 2 exostoses (multiple) 2 N78831 0.7574425 7 7 UNK R70037 0.7574429 8 4 developmentally regulated GTP-binding pr AA488336 0.757444 1 8 ESTs R76549 | R762 0.75847 14 4 cysteine dioxygenase, type I AA497033 0.7584

10 4 1 interleukin 2 receptor, gamma (severe co N54821 0.758425 12 11 ESTs H48537 0.758426 11 10 ESTs R96525 0.758430 2 10 UNK H67749 0.75848 6 10 Homo sapiens PNAS-123 mRNA, complete cds R38222 0.75936

31 13 12 UNK N55081 0.759364 3 12 LRP16 protein W52182 | AA2 0.760325 1 1 phosphate cytidylyltransferase 2, ethano R22219 0.760328 3 3 stromal cell-derived factor 1 AA447115 0.76032

16 2 10 ESTs N51056 0.7603217 13 8 hypothetical protein FLJ20037 R71627 0.7603230 7 9 tumor protein D52-like 2 R06254 0.760326 5 11 ESTs R22818 0.761288 9 6 UNK R66057 0.76128

11 12 5 heat shock protein 75 AA497020 0.761288 6 3 protein phosphatase 3 (formerly 2B), cat AA453998 0.762249 9 1 RAN binding protein 1 R40127 0.76224

16 13 4 thyroid autoantigen 70kD (Ku antigen) AA486207 0.7622425 2 11 UNK R22341 0.7622429 13 1 thyrotropin-releasing hormone AA069596 0.7622429 2 2 cadherin 12, type 2 (N-cadherin 2) N74018 0.762243 7 10 coagulation factor II (thrombin) H65052 0.76324 13 8 ESTs, Moderately similar to ALU8_HUMAN A T91282 0.76329 10 10 Homo sapiens mRNA for FLJ00038 protein, R36662 0.7632

13 10 11 UNK N71473 0.7641617 14 8 Homo sapiens mRNA; cDNA DKFZp434C136 (fr W76547 0.7641623 13 1 sodium channel, nonvoltage-gated 1 alpha AA458982 0.7641626 8 7 hypothetical protein FLJ10339 N95450 0.7641629 7 5 sarcoglycan, alpha (50kD dystrophin-asso AA460823 0.764163 11 11 ESTs H52251 | BF70 0.765124 14 12 EST R95778 0.765125 12 10 ESTs R27836 0.765127 1 6 vitiligo-associated protein VIT-1 AA487235 0.765129 11 1 protein tyrosine phosphatase, receptor t H82419 0.76512

13 12 12 ESTs T95314 0.7651218 1 10 EST H93608 0.7651224 7 11 ESTs H69876 0.7651228 2 10 ESTs H95239 0.765129 11 11 EST H48270 0.76608

31 8 6 carboxypeptidase E AA461513 0.7660832 7 6 ESTs R02800 0.766082 3 12 UNK AA464582 0.767045 6 9 ESTs N57848 0.7685 13 12 Homo sapiens cDNA FLJ12583 fis, clone NT N53764 0.768


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9 10 11 HpaII tiny fragments locus 9C R96881 0.76816 10 10 ESTs R68219 0.76826 5 6 f-box and leucine-rich repeat protein 11 W56287 0.76832 6 13 EMPTY EMPTY 0.7681 8 11 Homo sapiens cDNA FLJ11095 fis, clone PL H57540 0.768962 11 7 seven in absentia (Drosophila) homolog 2 AA029042 0.76896

16 11 3 ubiquitin specific protease 7 (herpes vi AA064638 0.7689621 3 4 zinc finger protein 258 AA280677 0.768963 8 9 ESTs, Moderately similar to ALU5_HUMAN A H69049 0.769923 7 11 UNK R86949 0.769928 10 9 EST R08157 0.76992

11 9 9 hypothetical protein FLJ20311 T99312 0.7699213 5 13 EMPTY EMPTY 0.7699220 13 3 GA-binding protein transcription factor, H91651 0.7699231 10 10 ESTs, Weakly similar to ALU1_HUMAN ALU S R26813 0.7699232 11 7 ATP-binding cassette, sub-family F (GCN2 N95462 0.769923 3 11 ESTs R99287 0.770889 13 8 ESTs, Weakly similar to T00351 hypotheti R16116 0.77088

18 4 5 glycophorin B (includes Ss blood group) AA455338 0.7708820 1 2 formyl peptide receptor-like 1 R79948 0.770884 11 11 IL-17B receptor H90761 0.771846 13 1 MADS box transcription enhancer factor 2 AA234897 0.77184

14 1 6 collagen, type XIV, alpha 1 (undulin) AA167223 0.7718416 13 9 ESTs R09557 0.771843 10 11 ESTs, Highly similar to ALU2_HUMAN ALU S R02323 0.7728

27 3 7 eukaryotic translation initiation factor N73769 0.772830 9 6 ESTs H53291 0.772825 10 3 integrin, alpha 8 R87964 0.7737631 1 3 colony stimulating factor 3 receptor (gr AA458507 0.773766 6 5 signal sequence receptor, delta (translo AA486261 0.77472

24 11 9 UNK R08275 0.7747227 2 7 Homo sapiens clone IMAGE:112577 mRNA seq T91249 0.7747227 14 8 ESTs N73873 0.7747216 8 1 myosin, light polypeptide 5, regulatory AA463986 0.7756831 4 4 ligase I, DNA, ATP-dependent AA291715 0.775683 12 5 tubulin-specific chaperone e AA504618 0.776646 10 8 ESTs R02819 0.77664

21 13 1 thromboxane A synthase 1 (platelet, cyto R76437 0.7766429 3 9 ESTs R32751 0.7766416 2 7 ESTs T95054 0.777619 12 1 plasminogen-like T67549 0.77764 12 12 p21 (CDKN1A)-activated kinase 3 R26396 0.77856

27 12 1 UNK T97181 0.7785627 14 13 root cap 1 Cont-7 0.778562 4 11 mannosyl (alpha-1,6-)-glycoprotein beta- H73014 0.779526 2 8 ESTs R10682 0.77952

19 8 11 thyroid receptor interacting protein 15 R36006 0.7795230 1 10 ESTs H66665 0.779525 8 11 ESTs N70298 0.78048

25 1 10 ESTs H53878 0.7804825 6 12 v-yes-1 Yamaguchi sarcoma viral oncogene N36882 0.7804827 4 7 Homo sapiens cDNA: FLJ21686 fis, clone C H94058 0.78048


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3 10 8 ESTs H57166 0.7814415 2 2 phosphomevalonate kinase H09819 0.7814417 3 6 transcription factor 6-like 1 (mitochond AA449118 0.7814430 13 2 glycogenin AA411679 0.7814431 11 3 myotubularin related protein 6 N49192 0.7814431 5 10 ESTs R98592 0.781442 9 3 B-cell CLL/lymphoma 6, member B (zinc fi N78611 0.7824

20 6 8 UNK T90292 0.782424 14 5 proteasome (prosome, macropain) 26S subu AA403126 0.782424 13 9 ESTs, Moderately similar to ALU7_HUMAN A H83000 0.78248 12 10 ESTs R72597 0.78336

30 6 11 ring finger protein 3 R06253 0.783362 6 1 proteolipid protein 2 (colonic epitheliu AA464528 0.784325 5 12 EST H74179 0.784329 7 13 EMPTY EMPTY 0.78432

10 7 8 ESTs T89998 0.7843210 8 12 Homo sapiens MSH55 gene, partial cds; an AA460293 0.7843223 5 1 v-akt murine thymoma viral oncogene homo AA457097 0.7843224 2 6 UNK R95780 0.7843232 11 8 hypothetical protein DKFZp566I133 N59523 0.7843221 4 4 MADS box transcription enhancer factor 2 AA282537 0.7852824 12 10 ESTs R71075 0.7852824 1 11 ESTs, Weakly similar to aldolase B [H.sa T98253 0.7852828 11 4 UNK AA487526 0.7852830 14 9 hypothetical protein FLJ12383 R08846 0.7852832 11 6 ESTs, Moderately similar to ALU7_HUMAN A R08755 0.785285 2 2 ALL1-fused gene from chromosome 1q AA456008 0.786247 7 7 ESTs, Moderately similar to ALU1_HUMAN A R16833 0.78624

15 11 3 sema domain, seven thrombospondin repeat AA436152 0.7862415 12 4 COX10 (yeast) homolog, cytochrome c oxid H21869 0.7862428 3 10 ESTs N33590 0.786247 14 6 Human clone 23909 mRNA, partial cds H18436 0.7872

10 6 1 indoleamine-pyrrole 2,3 dioxygenase AA478279 0.787221 7 3 KIAA0761 protein H51262 0.787229 13 4 damage-specific DNA binding protein 2 (4 AA410404 0.787230 13 7 peptidylprolyl isomerase E (cyclophilin N78843 0.787231 7 10 ESTs R99297 0.787232 6 10 hypothetical protein from clone 24774 H79138 0.78722 6 7 nitrilase 1 R06583 0.788165 12 9 ESTs R07631 0.78816

19 5 10 EST H58237 0.7881628 8 2 CD24 antigen (small cell lung carcinoma H59916 0.788166 4 9 EST H27560 0.78912

25 9 4 corticotropin releasing hormone receptor H07089 0.7891226 13 2 amyloid beta (A4) precursor protein-bind R55789 0.789122 11 12 kinectin 1 (kinesin receptor) H97775 0.79008

10 11 6 matrix Gla protein N53038 0.7900816 9 6 ESTs, Weakly similar to FAAH_HUMAN FATTY T70327 0.7900820 10 13 EMPTY EMPTY 0.7900821 10 5 paternally expressed 3 AA459941 0.790082 7 2 cisplatin resistance associated W72697 0.791046 10 9 ESTs R07444 0.79104


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12 1 10 ESTs H94884 0.7910415 11 4 deoxythymidylate kinase (thymidylate kin AA464256 0.7910417 5 11 UNK H75763 0.7910421 11 6 UNK AA150918 0.7910430 5 6 calcineurin-binding protein calsarcin-1 AA065090 0.7910417 14 11 ESTs H53118 0.79218 6 12 ESTs, Weakly similar to ALU1_HUMAN ALU S N34345 0.79227 10 11 ESTs, Weakly similar to ALU7_HUMAN ALU S R97735 0.7923 4 1 SEC14 (S. cerevisiae)-like 1 R55993 0.792968 1 10 ESTs H77697 0.79296

10 4 2 folate receptor 2 (fetal) AA453816 0.7929630 11 9 hypothetical protein DKFZp762L1710 R07446 0.792968 10 1 mal, T-cell differentiation protein AA227885 0.793928 10 2 neuregulin 1 R72075 0.793928 6 6 ESTs H68655 0.79392

16 3 11 ESTs, Highly similar to KIAA1167 protein R98433 | R984 0.793928 2 7 ESTs, Weakly similar to ALU7_HUMAN ALU S H94882 0.794888 3 12 ESTs, Weakly similar to putative p150 [H N54803 0.79488

15 11 11 ESTs, Moderately similar to ALU5_HUMAN A R93412 0.7948821 5 1 wingless-type MMTV integration site fami T99055 0.7948823 14 10 Homo sapiens cDNA: FLJ22165 fis, clone H R32361 0.7948829 5 9 Homo sapiens mRNA; cDNA DKFZp761H1023 (f R34382 0.794881 5 3 adenomatosis polyposis coli AA455997 0.795845 11 9 ESTs T95342 0.795846 4 10 ESTs H72259 0.79584

24 13 2 small inducible cytokine subfamily E, me H43129 0.795847 2 9 hypothetical protein FLJ20170 R33468 0.79689 8 13 EMPTY EMPTY 0.7968

10 1 9 ESTs R88999 0.796813 11 2 ATP-binding cassette, sub-family C (CFTR N68159 0.796821 7 8 ESTs T67022 0.79684 3 2 erythropoietin receptor H15574 0.79776

13 1 4 methylene tetrahydrofolate dehydrogenase AA480995 0.7977613 11 6 archain 1 AA598401 0.7977631 10 2 neuronal pentraxin I H22445 0.7977632 8 8 KIAA1096 protein R00104 0.7977616 4 4 KIAA0326 protein AA128278 0.798724 5 11 UNK N74930 0.799685 12 7 RING1 and YY1 binding protein AA027856 0.79968

17 2 10 ESTs N54502 0.7996822 6 9 ESTs H37799 0.7996831 13 2 HMT1 (hnRNP methyltransferase, S. cerevi N55480 0.7996831 14 2 histamine N-methyltransferase H53275 0.799681 6 12 spleen focus forming virus (SFFV) provir N66572 0.80064

19 14 3 peroxisomal acyl-CoA thioesterase AA447674 0.8006426 6 12 hypothetical protein FLJ23151 W48560 | AA2 0.800642 7 10 UNK N49892 0.8016

28 9 8 ESTs T83667 0.801630 5 7 ESTs, Highly similar to EF-9 [M.musculus H94467 0.801632 2 7 ESTs N39325 0.80162 12 8 ESTs T85248 0.802563 14 12 ESTs N76005 0.80256


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7 12 11 ESTs R94894 0.802568 10 8 ESTs N62588 0.80256

17 11 9 ESTs R97804 0.8025630 13 12 ESTs R26581 0.802562 8 3 HCGI protein AA054321 0.80352

10 12 2 chromosome X open reading frame 6 R08271 0.8035224 1 5 histidine ammonia-lyase W86776 0.8035230 5 9 hypothetical protein PRO2706 H63947 0.803522 11 10 ESTs N53560 0.804483 4 2 Hermansky-Pudlak syndrome AA418683 0.804484 9 11 isoprenylcysteine carboxyl methyltransfe H77506 0.80448

11 13 4 aspartoacylase (aminoacylase 2, Canavan N71653 0.8044811 9 7 putative transmembrane protein; homolog H79351 0.8044815 12 12 ESTs N67023 0.8044831 6 1 tuberous sclerosis 2 H37774 0.8044831 2 10 ESTs R96516 0.8044831 10 12 ESTs R06256 0.804489 14 11 ESTs H53263 0.80544

12 3 6 ESTs N73594 0.8054419 14 10 ESTs R89014 0.8054420 9 11 ESTs N69552 0.8054425 1 12 thyroid hormone receptor, alpha (avian e T66180 0.805446 10 7 Homo sapiens cDNA FLJ11699 fis, clone HE T86691 0.8064

12 9 7 cytochrome P450 isoform 4F12 R11209 0.806428 6 4 guanylate kinase 1 AA490902 0.80643 2 9 Homo sapiens cDNA FLJ11321 fis, clone PL R66534 0.807364 7 10 EST N52814 0.807367 5 6 serine (or cysteine) proteinase inhibito R71093 0.80736

25 1 9 ESTs H48502 0.8073630 4 7 KIAA1051 protein H51766 0.8073611 12 8 ESTs T67444 0.8083232 4 7 zinc finger protein 205 T67524 0.808322 1 11 UNK R76401 0.80928

14 9 1 mutS (E. coli) homolog 3 AA421716 0.8092815 8 3 GTP-binding protein overexpressed in ske AA418077 0.8092817 8 3 lectin, galactoside-binding, soluble, 9 AA434102 0.8092820 2 4 nucleolar phosphoprotein p130 AA488393 0.8092826 9 8 ESTs, Weakly similar to Z184_HUMAN ZINC T90462 0.8092828 10 3 guanosine monophosphate reductase AA410375 0.8102429 4 11 ESTs, Weakly similar to ALU8_HUMAN ALU S N59559 0.8102430 8 2 nuclear body protein Sp140 H66484 0.8102430 5 12 UNK H87363 0.8102431 9 3 gamma-aminobutyric acid (GABA) A recepto AA101225 0.810246 3 10 UNK H71314 0.81127 14 7 ESTs R09196 0.8112

19 8 10 ESTs H61684 0.811224 4 4 ESTs T97917 0.811224 6 5 microtubule-associated protein, RP/EB fa AA608576 0.811225 2 3 aldehyde dehydrogenase 7 family, member AA102646 0.811225 13 10 ESTs, Highly similar to ALU2_HUMAN ALU S R55307 | AI73 0.811231 4 5 growth differentiation factor 10 R52085 0.811231 12 9 EST T96077 0.8112


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31 14 10 EST R31426 0.81122 11 1 ESTs, Highly similar to transcriptional H05655 0.812162 14 8 Homo sapiens mRNA; cDNA DKFZp434D0218 (f T91215 0.812168 12 7 amyloid beta (A4) precursor protein-bind N92775 0.812169 4 13 EMPTY EMPTY 0.81216

21 8 6 centromere protein C 1 AA461496 0.812167 2 10 ESTs R96645 0.81312

15 13 3 UNK H93068 0.8131218 7 8 ESTs T85201 0.8131223 13 4 ESTs H00592 0.8131224 2 10 ESTs H69653 0.8131227 5 10 EST H57946 0.8131219 10 11 KIAA1096 protein H51043 0.814082 9 2 butyrophilin, subfamily 3, member A3 AA478585 0.815049 3 5 ras homolog gene family, member AA143437 0.81504

16 1 1 KIAA1128 protein AA114107 0.8150431 11 1 interferon-stimulated transcription fact AA291389 0.8150410 1 2 UNK AA085597 0.81614 2 9 ESTs N54790 0.81624 5 12 hypothetical protein FLJ20258 AA464955 0.81628 2 12 ESTs, Weakly similar to S10590 cysteine AA026030 0.81630 5 8 ESTs N63803 0.8164 7 12 ESTs, Weakly similar to cytochrome c-lik R33082 0.81696

19 6 10 ESTs H60317 0.8169626 3 7 glycoprotein M6B N59368 0.816964 9 10 chromosome 2 open reading frame 2 N64731 0.817924 6 11 activating transcription factor 7 W45531 0.81792

12 10 10 ESTs H93315 0.8179212 10 12 UNK H55784 0.8179216 7 13 EMPTY EMPTY 0.8179221 11 1 lactate dehydrogenase C AA453467 0.8179222 2 11 ESTs R07607 0.817923 14 2 KIAA0155 gene product AA133685 0.818884 14 13 Hu-Cox-1 Hu-Cox-1 0.818886 14 5 MSTP032 protein W81563 0.81888

20 1 4 TRAM-like protein T74606 0.8188825 2 7 ESTs T67045 0.8188827 2 3 aminolevulinate, delta-, synthase 1 AA447761 0.8188827 12 3 chaperonin containing TCP1, subunit 2 (b N38959 0.8188827 11 5 myeloid/lymphoid or mixed-lineage leukem N68327 0.8188832 5 12 flotillin 1 AA456611 0.8188815 10 11 F-box only protein 9 N73865 0.8198417 10 11 Homo sapiens cDNA FLJ11952 fis, clone HE AA147202 0.819848 5 10 ESTs N74085 0.8208

10 6 3 phosphodiesterase 6G, cGMP-specific, rod AA074148 0.820811 4 8 signal transducer and activator of trans R05931 0.820822 7 10 UNK H78182 0.82083 5 10 ESTs H58781 0.821764 11 2 T-box 2 N99243 0.821765 12 11 ESTs R92962 0.821766 4 12 olfactory receptor, family 7, subfamily AA620759 0.82176

10 5 13 EMPTY EMPTY 0.82176


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11 12 9 UDP-N-acetyl-alpha-D-galactosamine:polyp R00496 0.8217628 1 1 necdin (mouse) homolog R51580 0.8217630 12 3 phosphoinositide-3-kinase, class 3 AA456101 0.821763 3 3 aldehyde dehydrogenase 9 family, member R46816 0.822729 5 13 EMPTY EMPTY 0.82272

11 4 7 ESTs R99288 0.8227215 11 6 CD69 antigen (p60, early T-cell activati AA279755 0.822723 12 8 hypothetical protein FLJ23047 R54972 0.823684 14 4 serine/threonine kinase 2 AA496013 0.823688 14 11 ESTs H68932 0.82368

14 11 5 baculoviral IAP repeat-containing 1 H21072 0.8236818 3 6 Homo sapiens DC49 mRNA, complete cds W35283 0.8236823 4 1 valyl-tRNA synthetase 2 AA457123 0.8236825 11 1 protein tyrosine phosphatase, receptor t R38343 0.8236826 2 3 protein kinase, cAMP-dependent, regulato AA180007 0.8236829 7 7 ESTs R99579 0.8236816 12 5 mab-21 (C. elegans)-like 1 H18838 0.8246423 3 4 lysosomal-associated membrane protein 1 H28973 0.8246425 11 10 ESTs R37975 0.8246429 8 9 ESTs, Weakly similar to ALU7_HUMAN ALU S R08165 0.8246415 13 5 neuropeptide Y receptor Y1 R43817 0.825617 5 12 v-raf-1 murine leukemia viral oncogene h N25425 0.825625 2 6 UNK N32146 0.825629 5 7 solute carrier family 25 (mitochondrial AA411554 0.825631 1 12 ESTs T97205 0.825624 7 13 EMPTY EMPTY 0.8265632 10 1 mannosyl (beta-1,4-)-glycoprotein beta-1 H47026 0.826565 13 9 ESTs T96871 0.82752

10 4 8 ESTs R32648 0.8275213 10 12 Homo sapiens mRNA; cDNA DKFZp564D016 (fr R28280 0.8275214 8 12 ESTs, Weakly similar to WDNM RAT WDNM1 P W52432 | AA2 0.8275215 9 12 ESTs, Moderately similar to NRD2 convert H78796 0.8275216 3 3 succinate dehydrogenase complex, subunit T70043 0.8275218 4 3 plasminogen T73090 0.8275228 12 2 adaptor-related protein complex 4, mu 1 H11763 0.8275215 1 10 UNK N53453 0.8284822 5 10 ESTs H72914 0.8284824 4 8 ESTs R12689 0.8284828 5 8 ESTs, Moderately similar to ALU1_HUMAN A R10159 0.8284832 5 6 UNK R91296 0.828485 14 7 hypothetical protein FLJ22548 similar to W96184 0.82944

12 9 6 hyothetical protein R96507 0.8294415 9 7 hydroxysteroid (17-beta) dehydrogenase 7 N58305 0.8294417 4 7 NICE-5 protein R48169 0.8294417 13 9 ESTs R00527 0.8294420 6 2 chromatin assembly factor 1, subunit B ( AA426096 0.8294422 3 12 UNK AA169176 0.8294425 5 7 protein kinase, lysine deficient 1 R10088 0.8294425 3 10 ESTs H55897 0.8294416 2 8 ESTs R19395 0.830419 2 12 UNK N/A 0.830429 9 2 glycosylphosphatidylinositol specific ph N69672 0.8304


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2 10 13 EMPTY EMPTY 0.8313612 6 10 SH3 domain binding glutamic acid-rich pr N52254 0.8313615 2 3 collagen, type IV, alpha 4 H68555 0.8313630 8 7 hypothetical protein FLJ22002 R48248 0.831366 9 11 UNK T81580 0.83232

10 9 8 ESTs T90641 0.8323221 11 10 ESTs, Weakly similar to ALU6_HUMAN ALU S R26289 0.8323224 5 13 EMPTY EMPTY 0.8323218 4 11 Williams-Beuren syndrome chromosome regi R10117 0.8332810 10 3 glycogen synthase 1 (muscle) H08732 0.8342415 1 7 ESTs W47254 0.8342416 4 3 SRY (sex determining region Y)-box 2 AA451892 0.8342416 3 4 KIAA0342 gene product AA126869 0.8342425 11 4 CD8 antigen, alpha polypeptide (p32) AA443584 0.834248 4 4 dynein, cytoplasmic, heavy polypeptide 1 AA410454 0.8352

13 1 2 ATP-binding cassette, sub-family B (MDR/ R83876 0.835225 6 8 Homo sapiens clone 25232 mRNA sequence N41041 0.835228 13 3 GATA-binding protein 1 (globin transcrip R06446 0.835232 3 11 Homo sapiens cDNA FLJ10664 fis, clone NT R96310 0.835226 13 1 solute carrier family 17 (sodium phospha H60423 0.8361628 11 7 ESTs, Highly similar to 1819485A CENP-E H94466 0.8361617 9 5 ESTs AA250771 0.8371222 7 7 ESTs, Moderately similar to A36169 trans N55274 0.8371232 12 8 hypothetical protein R78548 | R784 0.837127 12 7 ESTs R97089 0.838088 13 3 L-3-hydroxyacyl-Coenzyme A dehydrogenase T65407 0.83808

13 13 10 ESTs R28345 0.8380821 14 12 Homo sapiens cDNA FLJ11660 fis, clone HE N54425 0.8380829 11 12 ESTs, Moderately similar to ALU4_HUMAN A R63911 0.8380811 10 12 cyclin D1 (PRAD1: parathyroid adenomatos R68272 0.8390412 8 6 hypothetical protein R11669 0.8390428 4 13 EMPTY EMPTY 0.8390410 12 8 ESTs T96930 0.8417 14 12 ESTs N74348 0.8423 1 7 KIAA0957 protein N68497 0.841 11 9 ESTs, Moderately similar to ALU2_HUMAN A T99792 0.840966 14 1 potassium voltage-gated channel, subfami AA427490 0.840967 8 7 BANP homolog, SMAR1 homolog N53581 0.84096

11 5 11 UNK R27412 0.8409614 12 8 Homo sapiens clone IMAGE:113399 mRNA seq T78463 0.840961 13 9 ESTs H65776 0.841928 5 2 clathrin, heavy polypeptide (Hc) H54288 0.84192

12 5 6 Homo sapiens mRNA; cDNA DKFZp761E2216 (f AA015850 0.8419219 11 9 ESTs, Weakly similar to ALU7_HUMAN ALU S R00151 0.8419220 3 11 ESTs H79650 0.8419227 8 9 ESTs, Weakly similar to ALU1_HUMAN ALU S T98846 0.8419228 8 12 hypothetical protein FLJ20425 AA063624 0.8419215 12 9 ESTs, Weakly similar to ALU7_HUMAN ALU S T96132 0.8428816 2 12 synaptogyrin 1 AA007632 0.8428817 8 2 KIAA0349 protein N51858 0.8428817 12 4 chemokine (C-C motif) receptor 2 H58254 0.8428830 6 8 Homo sapiens cDNA: FLJ22586 fis, clone H H94845 0.84288


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5 9 2 peroxisome proliferative activated recep AA088420 0.8438423 6 8 ESTs T67161 0.8438431 3 8 ESTs R09411 0.843844 10 2 UNK T71887 0.8448

21 9 9 ESTs R01161 0.844827 2 8 DKFZP564L0862 protein R02586 0.84489 10 13 EMPTY EMPTY 0.84576

18 4 2 ESTs H62396 0.8457632 12 9 EST R08598 0.8457613 12 10 Homo sapiens cDNA FLJ20767 fis, clone CO W52186 0.8467228 5 12 hypothetical protein FLJ22009 AA404276 0.8467230 5 11 ESTs N68576 0.846725 7 1 UNK T67270 0.84768

28 12 5 KIAA0121 gene product AA504515 0.8476828 14 12 splicing factor, arginine/serine-rich 2, R85367 0.8476829 7 9 uncharacterized hypothalamus protein HBE W60582 0.847687 7 2 lectin, galactoside-binding, soluble, 8 R97308 0.848649 13 2 solute carrier family 7 (cationic amino AA418078 0.84864

19 1 6 spleen focus forming virus (SFFV) provir N66572 0.8486421 2 6 UNK AA490946 0.8486416 4 11 CGI-119 protein H78365 0.849618 6 2 ESTs R15934 0.849619 10 7 EST N38967 0.84964 2 5 glutamic-oxaloacetic transaminase 1, sol H22856 0.85056

10 2 9 ESTs H93319 0.8505611 6 10 ESTs H60445 0.8505611 14 11 ESTs H54189 0.8505612 4 4 zinc finger protein 184 (Kruppel-like) AA455657 0.8505616 6 7 ESTs N68557 0.8505620 4 11 ESTs R09201 0.850563 9 8 Homo sapiens cDNA FLJ20394 fis, clone KA N50936 0.851524 1 6 UNK AA071122 0.85152

14 3 9 ESTs N68447 0.8515226 5 5 DNA segment, numerous copies, expressed AA278698 0.8515210 10 2 fatty acid synthase H50323 0.8524812 10 3 glucose-6-phosphatase, catalytic (glycog T98887 0.8524812 7 11 CCR4-NOT transcription complex, subunit N34048 0.8524822 8 2 lunatic fringe (Drosophila) homolog R56562 0.8524822 1 3 TXK tyrosine kinase H12312 0.8524828 1 9 ESTs H97748 0.852482 2 2 fibroblast growth factor receptor 3 (ach AA419620 0.853446 8 11 hypothetical protein DKFZp586K0717 T85091 0.85344

13 5 6 cut (Drosophila)-like 1 (CCAAT displacem AA292536 0.8534422 5 9 peroxisomal biogenesis factor 11A H53572 0.8534428 8 13 EMPTY EMPTY 0.853443 7 2 KIAA0352 gene product R80197 0.8544

17 11 1 protein tyrosine phosphatase, receptor t H74265 0.854412 10 1 intercellular adhesion molecule 5, telen R87763 0.8553619 2 2 homeo box A9 AA496921 0.8553620 1 7 ESTs R96604 0.8553621 10 9 UNK T95125 0.8553622 13 13 EMPTY EMPTY 0.85536


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8 14 8 UNK T81301 0.8563222 6 10 ESTs H77395 0.8563230 10 9 UNK R07324 0.8563217 10 7 zinc finger protein 238 R79723 0.8572824 5 9 ESTs R33354 0.8572827 6 10 A kinase (PRKA) anchor protein 11 N70003 0.8572831 3 11 DKFZP434B168 protein N62684 0.857282 8 10 ESTs R92163 0.85824

16 2 6 HSPC002 protein T67535 0.8582421 7 2 interleukin 16 (lymphocyte chemoattracta AA454732 0.8582424 6 2 UNK R52789 0.8582430 7 6 myosin regulatory light chain interactin AA487191 0.8582432 11 2 RNA binding motif protein 6 H50677 0.8582432 3 6 UNK N62461 0.85924 11 13 EMPTY EMPTY 0.860169 9 13 EMPTY EMPTY 0.86016

10 11 12 ecotropic viral integration site 5 H51372 0.8601610 2 13 EMPTY EMPTY 0.8601621 1 1 UNK R44822 0.8601625 14 8 Homo sapiens mRNA; cDNA DKFZp586L0120 (f R63343 0.8601610 12 9 ESTs, Weakly similar to ALU4_HUMAN ALU S R92412 0.8611218 2 12 Rho GTPase activating protein 8 AA037410 0.8611226 11 12 Homo sapiens cDNA FLJ13474 fis, clone PL H82532 0.861122 10 4 UNK T67270 0.86208

17 2 12 S-phase response (cyclin-related) R51052 0.8620818 10 7 nemo-like kinase R70769 0.8620819 10 8 spindle pole body protein N54552 0.8620820 1 8 mannosidase, alpha, class 1A, member 2 R78550 | R785 0.8620832 10 3 splicing factor, arginine/serine-rich 2, H78241 0.862089 5 6 lymphocyte-specific protein 1 T90632 0.86304

11 11 8 ESTs H61089 0.8630425 9 7 ATPase, Class VI, type 11B R95887 0.8630427 10 7 MSTP032 protein W81563 0.863041 5 2 UNK R91568 0.8644 13 3 fumarylacetoacetate H44956 0.864

22 1 12 platelet-derived growth factor receptor, H23235 0.86423 11 11 ESTs R93153 0.86432 13 13 EMPTY EMPTY 0.8644 13 2 pleckstrin homology, Sec7 and coiled/coi AA464957 0.864964 12 10 ESTs R87110 0.864965 14 9 DKFZP434I116 protein H58884 0.86496

22 2 10 Homo sapiens PRO1851 mRNA, complete cds N58282 0.8649623 14 2 heterogeneous nuclear ribonucleoprotein AA454880 0.8649624 13 6 transmembrane trafficking protein AA448691 0.8649631 6 9 EST R35979 0.8649621 1 4 natural killer cell transcript 4 AA458965 0.8659221 8 4 keratin 10 (epidermolytic hyperkeratosis AA479882 0.8668826 6 9 ESTs, Moderately similar to ALU1_HUMAN A R91986 0.8668830 11 10 ESTs R67336 0.866883 13 11 UNK H53053 0.867846 6 6 ESTs R07160 0.867847 13 8 hypothetical protein MGC5178 N55087 0.86784


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11 3 12 v-abl Abelson murine leukemia viral onco H81821 0.8678418 11 6 UNK N53177 0.867844 11 9 UNK R21262 0.86887 11 5 hypothetical protein FLJ10747 R53973 0.8688

11 1 10 ESTs N38825 0.868824 7 3 hematopoietic cell-specific Lyn substrat AA424575 0.868825 5 12 FYN oncogene related to SRC, FGR, YES N22980 0.86885 11 8 ESTs R16069 0.869765 4 12 hypothetical protein FLJ10390 H20045 0.86976

13 3 7 px19-like protein H73484 0.8697615 1 11 ESTs, Highly similar to ARGI_HUMAN ARGIN N57655 0.8697619 1 11 hypothetical protein FLJ20022 N79237 0.8697622 6 2 Lysosomal-associated multispanning membr AA406585 0.8697628 1 4 KIAA0062 protein T74699 0.869763 1 4 splicing factor, arginine/serine-rich 5 AA598965 0.870728 10 4 uteroglobin T63761 0.87072

11 4 11 ESTs, Weakly similar to ALU1_HUMAN ALU S N33593 0.8707213 7 13 EMPTY EMPTY 0.8707222 6 3 signal recognition particle receptor ('d AA598621 0.8707228 12 6 uncharacterized bone marrow protein BM04 H50667 0.870721 10 12 UNK H90746 0.871684 3 1 integrin, alpha 2 (CD49B, alpha 2 subuni AA463257 0.871684 1 5 butyrate response factor 2 (EGF-response AA480880 0.871689 13 4 biglycan R77227 | R772 0.87168

29 10 2 paxillin AA430574 0.871681 6 13 EMPTY EMPTY 0.87264

19 12 3 carboxypeptidase Z AA427725 0.8726414 1 9 UNK R78713 0.873621 10 10 dual-specificity tyrosine-(Y)-phosphoryl R63623 0.873624 13 8 ESTs H78613 0.873630 12 6 hypothetical protein FLJ12707 N69388 0.87365 8 6 spastic paraplegia 7, paraplegin (pure a AA504482 0.87456

22 3 5 hemopoietic cell kinase AA149097 0.8745627 14 10 Homo sapiens cDNA: FLJ21333 fis, clone C AA026470 0.8745631 8 5 Notch (Drosophila) homolog 4 AA464852 0.874562 12 2 RE1-silencing transcription factor R32858 0.875524 2 1 melanoma antigen, family A, 10 R23725 0.87552

12 6 4 Rho guanine nucleotide exchange factor ( AA481277 0.8755215 10 7 KIAA0921 protein H41237 0.8755216 5 6 ESTs H53556 0.8755221 9 4 envoplakin AA029419 0.8755227 4 8 ESTs R05832 0.8755229 13 12 ESTs N53552 0.875522 1 7 Homo sapiens, Similar to RIKEN cDNA 4933 T85191 0.87648

15 14 4 cysteinyl-tRNA synthetase AA464147 0.876483 11 8 KIAA0467 protein R06603 0.877448 10 13 EMPTY EMPTY 0.877448 13 13 EMPTY EMPTY 0.87744

25 8 11 DKFZP586B2420 protein W84525 0.8774413 6 1 uracil-DNA glycosylase H15112 0.878420 5 6 hypothetical protein FLJ21562 H69683 0.87843 8 6 biliverdin reductase A AA192419 0.87936


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7 13 3 protein kinase, AMP-activated, gamma 1 n AA070495 0.8793623 11 10 ESTs R26855 0.8793621 10 8 ESTs R16079 0.8803217 11 4 CD59 antigen p18-20 (antigen identified H60549 0.8812822 4 3 spectrin, beta, erythrocytic (includes s AA011320 0.8812827 4 10 ESTs H58001 0.8812828 7 3 highly charged protein H95641 0.881282 1 13 EMPTY EMPTY 0.882244 11 5 hypothetical protein FLJ20085 AA458840 0.882247 1 3 ESTs R31701 0.88224

10 3 12 Homo sapiens CDA02 mRNA, complete cds AA046328 0.8822422 13 3 biphenyl hydrolase-like (serine hydrolas R54850 0.8822424 9 6 FOS-like antigen-1 T89996 0.8822426 10 10 EST R83312 0.8822430 1 12 tyrosinase-related protein 1 AA424996 0.8822431 5 11 ESTs N94536 0.8822421 12 12 EphB1 N93797 0.883229 4 6 cytochrome P450, 51 (lanosterol 14-alpha AA477781 0.88324 5 12 ESTs, Weakly similar to ALU1_HUMAN ALU S H62009 0.884166 13 10 hypothetical protein FLJ20189 R70005 0.884168 8 12 cellular retinoic acid-binding protein 1 AA454702 0.88416

15 12 3 CREB binding protein (Rubinstein-Taybi s AA022480 0.8841620 1 3 proline arginine-rich end leucine-rich r AA434067 0.8841621 9 5 serine protease inhibitor, Kunitz type, AA458849 0.8841628 11 6 androgen induced protein R01070 0.8841629 2 1 neuronal Shc W93413 0.8841632 1 7 ESTs, Weakly similar to S71752 giant pro T96830 0.884162 12 9 GCN1 (general control of amino-acid synt N69521 0.885123 14 1 phosphatidylinositol glycan, class A (pa AA424735 0.885124 8 3 ESTs R89567 0.88512

12 2 3 procollagen-proline, 2-oxoglutarate 4-di AA457671 0.8851212 4 5 UNK AA457027 0.8851214 5 11 choroideremia-like (Rab escort protein 2 R92717 0.8851216 4 8 ESTs N63823 0.8851217 3 7 Homo sapiens, GAS2-related on chromosome R51206 0.8851217 12 11 ESTs N54036 0.8851217 12 12 desmoplakin (DPI, DPII) H90899 0.8851220 6 1 IMP (inosine monophosphate) dehydrogenas R52542 0.8851225 13 11 ESTs H54623 0.8851232 13 12 ESTs R16543 0.885128 12 6 protein disulfide isomerase-related prot R01669 0.88608

16 5 4 PDZ domain containing guanine nucleotide AA488900 0.8860816 10 9 ESTs R08141 0.8860823 7 7 ESTs, Weakly similar to ALU1_HUMAN ALU S H78331 0.8860832 1 9 ESTs R68654 0.886088 4 8 ESTs H55966 0.88704

23 1 11 ESTs, Moderately similar to JC4969 pig-c N55492 0.8870432 4 13 EMPTY EMPTY 0.887047 9 9 ESTs, Weakly similar to ALUF_HUMAN !!!! R01165 0.888

10 1 10 transcriptional co-activator with PDZ-bi H94043 0.88812 3 5 gamma-glutamyl carboxylase T68102 0.88823 11 9 ESTs R98822 0.888


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1 4 9 hypothetical protein FLJ10567 R63812 0.888962 1 8 S-phase kinase-associated protein 2 (p45 R22188 0.88896

21 11 5 putative chemokine receptor; GTP-binding R02740 0.8889631 13 11 ESTs N68486 0.8889625 8 6 ESTs AA102035 0.8899229 9 11 ESTs N70324 0.8899230 11 7 CATX-8 protein N93392 0.8899231 5 5 Homo sapiens cDNA: FLJ22272 fis, clone H AA495944 0.8899232 14 9 ESTs R12401 0.889921 6 1 UNK AA063631 0.890882 2 7 ESTs R02354 0.89088

19 4 10 ESTs H65232 0.8908821 6 13 EMPTY EMPTY 0.8908824 2 12 UNK N25353 0.8908827 9 11 KIAA0483 protein H77486 0.8908831 3 9 Homo sapiens cDNA FLJ11640 fis, clone HE R33476 0.8908815 13 9 ESTs T96998 0.8918415 6 10 ESTs H90692 0.8918422 1 8 ESTs, Moderately similar to ALU7_HUMAN A R09230 0.8918422 6 8 ESTs R07478 0.8918431 6 10 UNK H48445 0.8918411 9 12 ESTs R71393 | AI73 0.892812 2 13 EMPTY EMPTY 0.892816 6 5 Fas (TNFRSF6)-associated via death domai AA433944 0.892816 12 13 EMPTY EMPTY 0.892820 11 9 colon carcinoma related protein R62174 0.892824 13 5 zinc finger protein homologous to Zfp161 AA490538 0.892829 12 9 ESTs T95358 0.89286 5 7 ESTs, Highly similar to KIAA0535 protein R31591 0.893768 10 10 ESTs R68245 0.89376

28 2 3 prohibitin R60946 0.893761 7 3 latent transforming growth factor beta b R87406 0.894722 5 11 ESTs N59564 0.89472

15 9 4 Duffy blood group T82477 0.8947216 13 3 CD163 antigen AA401693 0.8947216 12 6 testis enhanced gene transcript (BAX inh H25560 0.8947216 8 10 ESTs, Weakly similar to ALUF_HUMAN !!!! H81048 0.8947229 4 2 secretory carrier membrane protein 3 R72518 0.894725 5 5 arylacetamide deacetylase (esterase) AA045320 0.895688 7 9 UNK R06862 0.89568

10 7 12 keratin, hair, acidic, 4 W49755 | AA2 0.8956814 3 12 Kruppel-associated box protein AA054757 0.8956816 9 12 eukaryotic translation initiation factor AA458487 0.8956819 14 9 ESTs R01272 0.8956819 13 10 KIAA1026 protein R63361 0.8956828 6 9 ESTs N68868 0.8956810 10 13 EMPTY EMPTY 0.8966414 6 7 deiodinase, iodothyronine, type I N74025 0.8966416 9 13 EMPTY EMPTY 0.8966427 7 8 HSPC154 protein H80103 0.8966430 2 11 KIAA0445 gene product R06258 0.8966430 11 13 EMPTY EMPTY 0.89664


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2 8 4 arsA (bacterial) arsenite transporter, A AA504809 0.89766 9 10 ESTs R67581 0.89763 12 4 polycystic kidney disease 2 (autosomal d R48232 0.898564 6 9 KIAA1345 protein N49231 0.89856

11 10 10 ESTs R53024 0.8985613 12 3 excision repair cross-complementing rode AA402486 0.8985620 12 3 UNK R39763 0.8985624 6 1 UNK AA397819 0.898562 13 5 KIAA0136 protein N73634 0.899528 9 8 ESTs, Moderately similar to ALU5_HUMAN A R11661 0.89952

19 14 5 vesicle-associated membrane protein 3 (c AA488635 0.8995221 11 11 ESTs N70970 0.8995231 7 7 ESTs H68724 0.8995225 6 9 ESTs R63922 0.9004830 2 3 tubulin, alpha 1 (testis specific) AA180742 0.9004831 5 9 ESTs R35661 0.9004832 11 9 hypothetical protein DKFZp434P0116 R08273 0.901442 3 6 hypothetical protein FLJ12549 H94469 0.9024

16 5 9 hypothetical protein FLJ21749 H95065 0.902419 10 9 hypothetical protein FLJ12876 R00037 0.902424 8 6 UNK H63090 0.902429 6 2 solute carrier family 16 (monocarboxylic R73608 0.902416 4 1 UNK N52474 0.9033616 6 1 killer cell lectin-like receptor subfami AA190627 0.9033621 5 6 cyclin A2 AA608568 0.903362 5 5 GATA-binding protein 4 AA431347 0.90432

10 12 11 EST H90848 0.9043212 11 7 Homo sapiens cDNA: FLJ21715 fis, clone C W02795 0.9043226 13 7 Homo sapiens scavenger receptor cysteine N69291 0.904327 6 5 UNK T62529 0.905287 2 12 ESTs R36628 0.90528

24 11 1 inositol(myo)-1(or 4)-monophosphatase 1 H90874 0.9052827 4 13 EMPTY EMPTY 0.9052832 13 1 UNK T98783 0.9052810 10 7 hypothetical protein FLJ11085 R10161 0.9062411 10 11 UNK T99074 0.9062425 14 7 potassium large conductance calcium-acti AA070026 0.9062426 4 11 EST H68719 0.9062432 13 3 FXYD domain-containing ion transport reg AA126009 0.906243 12 12 ESTs, Moderately similar to ALU7_HUMAN A H79007 0.90725 11 4 erythrocyte membrane protein band 7.2 (s R62817 0.90726 10 5 hypothetical protein PRO2706 AA496780 0.90726 1 6 ATP-binding cassette, sub-family C (CFTR R91503 0.90729 1 4 transcription factor CP2 AA488618 0.9072

10 5 11 ESTs N58065 0.907215 3 11 M-phase phosphoprotein 1 N63752 0.90723 9 11 hypothetical protein DKFZp547O146 T80848 0.908164 10 1 retinoic acid receptor responder (tazaro AA482067 0.90816

18 11 3 glutathione synthetase AA463458 0.9081622 11 3 SH3-domain GRB2-like 3 H50251 0.9081623 8 7 EST, Weakly similar to ORF2 contains a r R38239 0.9081632 13 11 ESTs H65839 0.90816


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1 13 5 huntingtin (Huntington disease) T64015 0.909123 6 9 amyotrophic lateral sclerosis 2 (juvenil R65826 0.90912

11 11 4 Homo sapiens cDNA FLJ11558 fis, clone HE R98008 0.9091216 12 2 eyes absent (Drosophila) homolog 2 AA402754 0.9091224 11 13 EMPTY EMPTY 0.9091228 2 6 EST W46976 0.9091231 7 4 solute carrier family 25 (mitochondrial W44701 0.9091213 4 4 glutamyl-prolyl-tRNA synthetase AA599158 0.9100822 4 9 ESTs H27519 | AI66 0.910083 8 5 dolichyl-phosphate mannosyltransferase p AA004759 0.911044 12 11 UNK H91164 0.911046 12 1 MAX protein N68443 0.911049 14 1 platelet/endothelial cell adhesion molec R22412 0.911042 6 8 ESTs T82259 0.912

17 1 5 cullin 4A AA598836 0.91219 5 8 hypothetical protein FLJ21603 T66929 0.9126 8 4 KIAA0233 gene product AA160695 0.912967 10 10 ESTs, Weakly similar to ALU7_HUMAN ALU S R26708 0.91296

12 2 9 UNK R91170 0.9129632 11 13 EMPTY EMPTY 0.912966 7 6 nuclear receptor co-repressor 1 T99086 0.91392

15 13 4 cytochrome P450, subfamily IIC (mephenyt R89492 0.9139215 14 12 genethonin 1 N55430 0.9139226 1 8 ESTs R22057 0.9139228 8 3 guanine nucleotide binding protein (G pr AA406364 0.9139231 14 5 myosin, heavy polypeptide 9, non-muscle T69926 0.913923 1 12 tumor necrosis factor receptor superfami H41522 0.914884 14 2 nescient helix loop helix 1 H09936 0.914887 8 12 BUP protein R16909 0.91488

11 9 4 ectonucleoside triphosphate diphosphohyd H13211 0.9148811 11 10 Wolfram syndrome 1 (wolframin) R53911 0.9148812 8 5 elastase 3B W45415 0.9148814 11 10 ESTs R67373 0.9148826 11 2 UNK H45618 0.9148826 11 7 hexosaminidase B (beta polypeptide) T63321 0.9148832 13 9 EST R09510 0.9148811 1 11 ESTs N80145 0.9158414 13 12 ESTs, Moderately similar to ALU7_HUMAN A R36114 0.915841 7 1 retinoblastoma-like 1 (p107) H84048 0.9168

12 10 9 ESTs R20803 0.916822 5 2 UNK H96850 0.91683 14 4 apolipoprotein D H15842 0.91776

18 2 10 ESTs, Weakly similar to ALU1_HUMAN ALU S H94332 0.9177629 8 8 ESTs T67520 0.9177630 8 1 nerve growth factor, beta polypeptide T52484 0.917769 8 11 ESTs R93394 0.91872

15 2 6 D component of complement (adipsin) AA233549 0.9187219 12 9 ESTs R00486 0.9187219 14 13 lipid transfer protein 6 Cont-5 0.9187214 8 8 ESTs R06119 0.9196819 14 7 protein tyrosine phosphatase, receptor t T67544 0.9196826 5 7 Homo sapiens similar to J KAPPA-RECOMBIN R16902 0.91968


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7 7 9 chromosome 1 open reading frame 21 N69286 0.9206416 10 8 Homo sapiens mRNA; cDNA DKFZp434I1820 (f N52394 0.9206419 11 6 UNK R85840 0.9206422 3 3 ESTs, Weakly similar to ubiquitous TPR m T50282 0.9206424 8 9 EST R06997 0.9206414 5 5 tafazzin (cardiomyopathy, dilated 3A (X- AA279440 0.921624 8 12 protein kinase, AMP-activated, beta 2 no N78582 0.921631 5 8 ESTs, Weakly similar to 2109260A B cell T67092 0.92165 3 12 ESTs R97031 0.92256

10 5 6 hypothetical protein FLJ13611 H56656 0.9225624 13 10 ESTs R73075 0.9225632 9 5 isocitrate dehydrogenase 3 (NAD+) gamma AA459380 0.9225632 11 10 src homology 3 domain-containing protein R68568 0.9225632 5 13 EMPTY EMPTY 0.922561 12 10 ESTs H60038 0.923527 5 1 uroporphyrinogen III synthase (congenita AA443089 0.92352

15 12 6 CD44 antigen (homing function and Indian AA283090 0.9235218 7 12 Human Chromosome 16 BAC clone CIT987SK-A N75498 0.9235220 8 1 UNK AA485677 0.9235226 10 9 eukaryotic translation initiation factor R17005 0.9235215 9 6 ESTs, Moderately similar to CAD8_HUMAN C R56219 0.9244815 13 6 serologically defined colon cancer antig AA161465 0.924481 5 1 SRY (sex determining region Y)-box 9 (ca AA400464 0.925445 10 13 EMPTY EMPTY 0.92544

14 5 10 UNK H72934 0.926416 12 10 ATPase, Class VI, type 11A H80708 0.926425 2 1 KIAA0182 protein H05099 0.926427 4 9 ESTs R63962 0.92646 5 9 ESTs R97050 0.927368 7 10 ESTs H80633 0.92736

16 3 1 protein phosphatase 2 (formerly 2A), reg AA427433 0.9273621 4 2 secretory carrier membrane protein 2 R32802 0.9273622 7 1 nuclear factor I/X (CCAAT-binding transc AA406269 0.9273627 1 12 vav 1 oncogene T65770 0.9273631 1 7 ESTs H77399 0.927361 9 13 EMPTY EMPTY 0.92832

15 13 7 ESTs, Highly similar to S58884 Ran-bindi N55016 0.9283227 14 2 KIAA0172 protein AA464605 0.928328 4 12 Homo sapiens, clone IMAGE:3947276, mRNA, W02772 | AA2 0.92928

11 7 8 KIAA0871 protein R94511 0.9292815 7 7 bladder cancer overexpressed protein H95824 0.929287 1 5 N-myristoyltransferase 1 AA476587 0.93024

19 8 7 MAP/microtubule affinity-regulating kina N62695 0.9302419 11 8 DHHC1 protein T67066 0.9302423 11 6 testis-specific kinase 1 AA461506 0.9302426 7 13 EMPTY EMPTY 0.930249 7 1 KDEL (Lys-Asp-Glu-Leu) endoplasmic retic T98559 0.9312

14 11 8 ESTs, Moderately similar to ALU2_HUMAN A H59057 0.931215 2 12 beta-1,3-N-acetylglucosaminyltransferase H00589 0.931224 3 7 ESTs H78135 0.93126 8 6 ESTs, Weakly similar to I38588 reverse t N63727 0.932166 7 9 ESTs R11532 0.93216


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13 14 13 EMPTY EMPTY 0.9321615 3 5 ESTs, Highly similar to PA2X_HUMAN CALCI T94293 0.9321622 1 9 TNF receptor-associated factor 5 AA102634 0.9321625 9 9 ESTs T98529 0.9321632 7 8 Homo sapiens cDNA FLJ11034 fis, clone PL T71250 0.9321620 11 1 syntaxin 7 T71551 0.9331220 12 9 ESTs R33606 0.9331232 7 4 KIAA0217 protein AA491206 0.933121 9 12 baculoviral IAP repeat-containing 2 AA702174 0.934083 14 7 Human DNA sequence from clone RP11-16L21 W72246 0.93408

14 3 13 EMPTY EMPTY 0.9340819 14 2 KIAA0170 gene product H68790 0.9340832 6 2 KIAA0022 gene product H60460 0.934084 9 8 KIAA0974 protein T83722 0.935048 8 5 serine threonine protein kinase AA521346 0.93504

10 14 5 KIAA0090 protein AA449957 0.935043 10 12 EST R78576 | R785 0.9366 7 12 ESTs, Weakly similar to JC5256 adipocyte AA148685 0.9367 4 1 v-kit Hardy-Zuckerman 4 feline sarcoma v N20798 0.9369 3 13 EMPTY EMPTY 0.936

17 9 11 ESTs R32847 0.93614 14 8 ESTs T80611 0.9369619 10 10 ESTs R52949 0.9369621 5 11 EST N66074 0.936964 7 5 exostoses (multiple) 1 AA487582 0.937928 4 5 translin AA460927 0.93792

12 12 8 UNK T91086 0.9379219 5 2 Rho guanine exchange factor (GEF) 12 AA479287 0.9379219 10 12 ESTs, Weakly similar to ALU1_HUMAN ALU S R63219 0.9379223 6 5 ephrin-B1 AA428778 0.937921 8 2 neuronal cell adhesion molecule R45583 0.93888

24 11 7 Homo sapiens mRNA; cDNA DKFZp564B1023 (f H55893 0.9388830 9 11 hypothetical protein PP3501 N73762 0.938885 8 9 ESTs N66842 0.93984

18 1 7 ESTs T97616 0.9398427 8 10 ESTs H69577 0.9398428 7 9 UNK N90913 0.940813 10 8 hypothetical protein FLJ21007 R54735 0.9417617 3 3 annexin A4 AA419015 0.9417626 6 5 gamma-glutamyltransferase-like activity AA150687 0.941767 14 1 Homo sapiens, clone IMAGE:3357927, mRNA, AA490477 0.94272

22 9 3 major histocompatibility complex, class R47979 0.9427230 12 13 EMPTY EMPTY 0.9427231 12 2 inositol 1,4,5-triphosphate receptor, ty AA479093 0.942725 4 13 EMPTY EMPTY 0.94368

12 9 12 GCN5 (general control of amino-acid synt AA455271 | AI 0.9436831 11 11 ESTs N55013 0.943684 9 5 ESTs, Moderately similar to PC1219 dihyd W88472 0.94464

14 2 10 ESTs H68171 0.9446423 3 8 ESTs T97992 0.944641 11 11 ESTs N74360 0.94566 9 5 UNK AA489017 0.9456


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15 14 10 ESTs R31671 0.945618 1 11 ESTs R67983 0.945623 10 11 UNK N73861 0.945628 10 9 RAD51 (S. cerevisiae) homolog (E coli Re N70010 0.945611 5 10 ESTs N62677 0.9465619 4 11 ESTs N59638 0.9465623 8 10 EST R99938 0.9465613 6 4 serine (or cysteine) proteinase inhibito AA486275 0.9475215 14 3 EBNA-2 co-activator (100kD) AA017383 0.9475222 10 11 UNK T86603 0.947525 7 2 ephrin-B3 AA485665 0.94848

23 3 11 ESTs N63693 0.9484815 9 11 ESTs N73749 0.9494426 4 9 EST R91409 0.949448 6 4 KIAA0218 gene product H15567 0.9504

12 8 13 EMPTY EMPTY 0.950418 4 1 isovaleryl Coenzyme A dehydrogenase AA464149 0.950421 5 13 EMPTY EMPTY 0.950426 7 3 NGFI-A binding protein 2 (ERG1 binding p AA434391 0.95043 3 8 transmembrane protein 1 R08766 0.951365 3 13 EMPTY EMPTY 0.951366 4 11 Homo sapiens cDNA: FLJ23137 fis, clone L H53269 0.95136

12 11 13 EMPTY EMPTY 0.9513619 7 12 presenilin 2 (Alzheimer disease 4) AA152294 0.9513625 8 1 retinal outer segment membrane protein 1 H84114 0.9513615 2 10 GRB2-associated binding protein 1 R96595 0.9523227 2 11 ESTs H63760 0.9523232 1 1 phosphoinositide-3-kinase, regulatory su R06567 0.952323 11 9 solute carrier family 22 (extraneuronal R08121 0.953288 12 13 EMPTY EMPTY 0.95328

10 10 1 sarcoma amplified sequence R45413 0.9532810 13 11 EST H91257 0.9532816 9 7 EHM2 gene H66834 0.9532819 12 4 tumor necrosis factor, alpha-induced pro AA457114 0.9532820 9 12 neuro-oncological ventral antigen 2 W35411 0.9532826 10 2 butyrophilin, subfamily 2, member A1 H68107 0.9532827 6 8 novel C3HC4 type Zinc finger (ring finge N71367 0.9532828 2 2 FK506-binding protein 2 (13kD) R75820 0.9532828 4 11 decidual protein induced by progesterone N55269 0.9532832 12 2 chromosome 11 open reading frame 8 AA020012 0.9532811 14 8 ESTs R99311 0.9542421 12 11 ESTs N54497 0.9542422 9 1 calcium channel, voltage-dependent, beta R92452 0.9542427 7 13 EMPTY EMPTY 0.954247 11 6 CD68 antigen AA421296 0.95616

11 3 10 ESTs H57111 0.9561614 11 7 TSC-22-like W57855 0.9561610 4 6 DKFZP564I122 protein T67049 0.9571221 4 5 UNK R45014 0.9571221 4 8 ESTs T97713 0.9571223 3 7 dihydrofolate reductase N52980 0.9571225 5 11 betaine-homocysteine methyltransferase 2 N76012 0.95712


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31 2 12 transmembrane protein 4 R22439 0.957128 12 9 ESTs R08762 0.95808

15 10 3 deleted in azoospermia AA129397 | AI 0.9580815 3 8 Homo sapiens clone IMAGE:121736 mRNA seq T97997 0.9580827 2 6 related to the N terminus of tre AA281057 0.958081 8 13 EMPTY EMPTY 0.962 10 9 KIAA1467 protein W35398 0.962 11 13 EMPTY EMPTY 0.964 3 8 ESTs R25159 0.965 1 13 EMPTY EMPTY 0.967 11 1 general transcription factor IIE, polype AA455964 0.96

11 10 3 coatomer protein complex, subunit alpha N54848 0.9612 4 8 ESTs H80107 0.9614 7 3 amyotrophic lateral sclerosis 2 (juvenil N55566 0.9614 12 10 Homo sapiens cDNA FLJ11027 fis, clone PL R68997 0.9615 8 13 EMPTY EMPTY 0.9616 8 9 zinc finger protein 202 R07016 0.9616 7 11 KIAA0713 protein R33832 0.9618 14 6 homeo box D1 W68537 0.9619 13 3 KIAA0409 protein AA447770 0.9619 4 9 CDA14 R64109 0.9620 1 1 aldehyde dehydrogenase 5 family, member H06676 0.9622 8 4 KIAA0157 protein AA453419 0.9622 11 4 vinculin AA486728 0.9622 4 13 EMPTY EMPTY 0.9623 7 2 protein AA479981 0.9624 2 13 EMPTY EMPTY 0.9625 11 9 hypothetical protein T99645 0.9632 12 13 EMPTY EMPTY 0.962 9 6 peflin R38031 0.960969 14 3 ras-related C3 botulinum toxin substrate N51095 0.96096

12 4 10 retinitis pigmentosa 2 (X-linked recessi N70013 0.9609610 2 11 EST H54764 0.9619214 9 7 Homo sapiens mRNA; cDNA DKFZp434N2116 (f H53879 0.9619220 7 2 coxsackie virus and adenovirus receptor N25352 0.9619221 3 5 COX11 (yeast) homolog, cytochrome c oxid AA457644 0.9619214 3 7 caspase 8, apoptosis-related cysteine pr H95655 0.9628818 10 8 ESTs R98070 0.9628823 6 9 ESTs R36086 0.9628826 4 3 platelet-derived growth factor beta poly T49540 0.9628827 7 12 claudin 3 AA039323 0.9628829 12 11 ESTs R93080 0.9628831 13 1 sorcin H60859 0.962886 8 1 nuclear transport factor 2 (placental pr N75595 0.96384

10 7 10 ESTs N49305 0.9638416 1 6 fibrinogen, gamma polypeptide T94279 0.963846 6 8 ESTs T69772 0.96487 6 12 abl-interactor 12 (SH3-containing protei N94992 0.9648

12 2 6 hypothetical protein FLJ20551 H61979 0.964823 10 9 ESTs, Moderately similar to ALU1_HUMAN A R91913 0.964829 8 2 solute carrier family 21 (prostaglandin AA037014 0.964830 10 12 KIAA0610 protein R33103 0.9648


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4 12 8 ESTs T91100 0.965766 8 2 glutamate dehydrogenase 1 AA017175 0.965767 1 12 EST R16977 0.965768 5 1 phosphoinositide-3-kinase, catalytic, al W72473 0.96576

10 4 3 serine (or cysteine) proteinase inhibito T49159 0.9657610 14 10 ESTs H48142 0.9657621 4 1 zinc finger protein 38 (KOX 25) AA088434 0.9657622 8 10 UNK N74059 0.9657629 9 4 enoyl-Coenzyme A, hydratase/3-hydroxyacy R02373 0.9657632 7 5 SPARC-like 1 (mast9, hevin) AA490471 0.965761 7 11 Pyruvate dehydrogenase complex, lipoyl-c N62460 0.966725 11 10 ESTs R27006 0.96672

13 14 9 EST T96919 0.9667216 8 4 KIAA0202 protein AA446924 0.9667218 2 2 fibronectin 1 R62612 0.9667226 1 7 ESTs, Moderately similar to ALU7_HUMAN A N52406 0.9667227 3 9 ESTs R63388 0.9667219 4 2 UNK H02158 0.9676827 10 5 UNK H75547 0.9676828 14 9 EST-YD1 protein R22919 0.9676829 6 8 ESTs T66898 0.9676830 13 9 ESTs R08773 0.9676810 10 5 ecotropic viral integration site 2A H93149 0.9686412 5 4 human immunodeficiency virus type I enha AA442780 0.9686421 10 3 chemokine (C-C motif) receptor 6 N57964 0.9686425 12 12 UNK H65984 0.9686426 13 10 hypothetical protein FLJ12668 H80100 0.9686430 14 13 EMPTY EMPTY 0.9686414 14 11 ESTs H66466 0.969626 14 12 hypothetical protein MGC10765 N58163 0.969628 9 2 ectonucleotide pyrophosphatase/phosphodi R38717 0.96964 8 11 centrosomal protein 1 H66030 0.970564 9 13 EMPTY EMPTY 0.97056

12 7 5 UNK T60075 0.9705618 9 6 protein kinase H11; small stress protein H57494 0.9705619 3 3 collagen, type III, alpha 1 (Ehlers-Danl T98612 0.9705627 14 4 arginase, liver AA447741 0.9705610 4 9 ESTs H64245 0.9715218 1 8 UNK R22065 0.9715218 5 13 EMPTY EMPTY 0.9715230 5 1 putative receptor protein AA053393 0.971525 6 13 EMPTY EMPTY 0.97248

14 12 7 ESTs, Weakly similar to RETROVIRUS-RELAT T70356 0.9724818 8 4 UNK AA455102 0.9724818 9 5 enolase 2, (gamma, neuronal) AA450123 0.9724823 2 10 ESTs R96586 0.972485 13 8 ESTs, Weakly similar to ALU7_HUMAN ALU S T70329 0.973446 4 8 Homo sapiens cDNA FLJ11953 fis, clone HE R19183 0.97344

12 3 7 hypothetical protein FLJ10879 R07637 0.9734422 7 3 N-deacetylase/N-sulfotransferase (hepara AA463635 0.9734422 4 10 EST H72247 0.9734423 4 11 EST N64716 0.97344


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23 12 13 EMPTY EMPTY 0.9734430 3 7 hydroxyacyl-Coenzyme A dehydrogenase/3-k T69767 0.973442 6 4 H2A histone family, member L AA452933 0.97446 14 8 ESTs R06160 0.97449 1 13 EMPTY EMPTY 0.9744

18 12 11 UNK H90134 0.97448 13 9 Homo sapiens clone IMAGE:297403, mRNA se N90167 0.97536

12 7 13 EMPTY EMPTY 0.9753614 9 5 ZYG homolog AA489714 0.9753619 11 12 EST N52517 0.9753621 13 8 Homo sapiens mRNA; cDNA DKFZp434I0812 (f AA479781 0.9753628 1 8 ESTs R22524 0.9753630 11 11 ESTs N68463 0.975369 12 13 EMPTY EMPTY 0.97632

20 9 3 peanut (Drosophila)-like 1 N92319 0.9763223 10 3 DNA fragmentation factor, 45 kD, alpha p AA487452 0.9763210 1 13 EMPTY EMPTY 0.9772814 2 6 protein kinase, AMP-activated, beta 1 no AA432271 0.9772814 3 10 KIAA0220 protein R91822 0.9772823 3 2 pescadillo (zebrafish) homolog 1, contai R37665 0.977285 9 7 EST AA030004 0.97824

18 14 5 membrane-bound transcription factor prot AA447394 0.978246 8 9 EST R06700 0.97928 12 12 UNK N51829 0.9792

12 4 13 EMPTY EMPTY 0.979219 7 10 ESTs H60689 0.979222 8 6 KIAA0217 protein AA491206 0.979225 10 12 hypothetical protein H41196 0.979230 10 6 hypothetical protein IMPACT T64930 0.979217 14 7 prothymosin, alpha (gene sequence 28) R10973 0.9801611 3 13 EMPTY EMPTY 0.9811224 5 1 protease, serine, 22 H03346 0.9811225 9 11 DEAD/H (Asp-Glu-Ala-Asp/His) box polypep N62675 0.9811232 4 11 UNK H53805 0.981127 14 8 hypothetical protein DKFZp564O1664 R83880 0.982088 14 1 glycine amidinotransferase (L-arginine:g R61229 0.98208

10 2 6 forkhead box C1 W94629 0.9820814 9 12 Homo sapiens cDNA FLJ14307 fis, clone PL AA427873 0.9820817 14 3 MAD (mothers against decapentaplegic, Dr AA424700 0.9820818 12 7 similar to arginyl-tRNA synthetase (argi T83997 0.9820829 3 8 ESTs N74915 0.9820831 8 13 EMPTY EMPTY 0.982085 6 3 casein kinase 2, alpha 1 polypeptide T98472 0.98304

11 12 6 seb4D AA459363 0.9830415 6 12 hypothetical protein FLJ20280 N74086 0.9830419 4 7 Homo sapiens mRNA; cDNA DKFZp762H185 (fr R92353 0.9830420 2 9 UNK R91132 0.9830424 10 2 ESTs, Highly similar to JC5931 high mobi H98218 0.9830428 12 13 EMPTY EMPTY 0.9830432 3 5 5-hydroxytryptamine (serotonin) receptor R55130 0.9830430 10 3 splicing factor 3a, subunit 1, 120kD T72628 0.98414 7 2 SET domain, bifurcated 1 R37131 0.98496


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13 11 4 UNK H81220 0.985927 4 4 latent transforming growth factor beta b AA424584 0.98688

10 6 10 spastic ataxia of Charlevoix-Saguenay (s N66003 0.9868818 7 3 Lutheran blood group (Auberger b antigen H24954 0.9868818 6 8 ESTs T97356 0.9868820 9 1 nuclear factor of activated T-cells, cyt AA402467 0.9868823 8 8 ESTs R16137 0.9868827 1 5 UNK AA452872 0.9868829 4 1 myeloid/lymphoid or mixed-lineage leukem N77807 0.9868832 9 11 ESTs R02295 0.986884 5 8 Homo sapiens clone IMAGE:110582 mRNA seq T90074 0.98784

12 3 9 Homo sapiens mRNA for KIAA1413 protein, H69709 0.9878419 13 8 UNK T68274 0.9878424 5 7 ESTs, Moderately similar to VWHU von Wil N65960 0.9878428 5 7 ESTs, Weakly similar to YOJ1_CAEEL HYPOT N66064 0.9878430 1 1 Epstein-Barr virus induced gene 3 AA495974 0.98883 14 6 Rho GDP dissociation inhibitor (GDI) bet AA487426 0.989765 7 5 novel RGD-containing protein AA449975 0.989768 5 8 RAB3A interacting protein (rabin3)-like T70580 0.98976

11 2 11 ESTs N52822 0.9897616 7 6 UNK T97023 0.9897622 7 8 hypothetical protein MGC5306 H47257 0.9897625 14 2 KIAA0205 gene product R91264 0.9897626 6 6 ankyrin 1, erythrocytic N63745 0.9897630 6 5 UNK AA449234 0.9897621 8 3 v-Ki-ras2 Kirsten rat sarcoma 2 viral on N95249 0.9907221 9 12 secretory carrier membrane protein 2 AA453114 0.9907224 1 9 UNK R68682 0.990721 13 10 brain and nasopharyngeal carcinoma susce H90882 0.991687 1 10 chromosome 20 open reading frame 1 H73968 0.99168

17 13 10 SRY (sex determining region Y)-box 4 R55640 | AI73 0.9916821 7 12 splicing factor, arginine/serine-rich 5 R73672 0.9916832 7 9 ESTs R06780 0.991684 14 7 ESTs T66794 0.992645 1 2 nuclear protein, marker for differentiat R43778 0.992647 12 8 clone FLB5214 R00432 0.99264

12 1 1 very long-chain acyl-CoA synthetase; lip AA464139 0.9926415 9 9 ESTs, Weakly similar to KIAA1204 protein H60022 0.9926419 12 12 ESTs H54285 0.9926421 14 11 hypothetical protein FLJ22263 similar to R94726 0.9926425 9 2 KIAA0336 gene product R00035 0.992641 6 2 low density lipoprotein-related protein AA464566 0.99366 6 7 ESTs, Weakly similar to A42926 L6 surfac R16547 0.9936

31 9 10 ESTs R25641 0.99365 12 4 decay accelerating factor for complement R09561 0.99456

14 4 13 EMPTY EMPTY 0.9945623 14 12 EST N55359 0.9945628 5 13 EMPTY EMPTY 0.9945621 7 6 cytochrome b-245, beta polypeptide (chro AA463492 0.9955225 2 13 EMPTY EMPTY 0.995528 9 13 EMPTY EMPTY 0.99648

14 9 6 ESTs H90890 0.99648


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14 13 10 EST R69975 0.9964816 10 13 EMPTY EMPTY 0.9964822 10 8 ESTs R09179 0.9964831 12 6 CD58 antigen, (lymphocyte function-assoc AA136271 0.996484 3 13 EMPTY EMPTY 0.99744

10 12 7 aquaporin 3 R92737 0.9974417 13 7 early endosome antigen 1, 162kD N66043 0.9974418 1 13 EMPTY EMPTY 0.9974421 10 2 pancreatic lipase-related protein 1 W45219 0.997446 12 2 low density lipoprotein-related protein R76808 0.99848 2 13 EMPTY EMPTY 0.9984

11 12 3 DKFZP434C128 protein AA062813 0.998419 2 1 KIAA0130 gene product N54470 0.998420 4 8 hypothetical protein FLJ12899 R91948 0.998424 2 3 surfactant, pulmonary-associated protein AA487571 0.99841 1 13 EMPTY EMPTY 0.999363 11 5 ubiquitin specific protease 4 (proto-onc AA454143 0.999368 11 8 ESTs N63691 0.99936

11 10 9 ESTs R11527 0.9993618 6 10 ESTs, Weakly similar to ALU1_HUMAN ALU S H79640 0.9993619 4 8 ESTs R05837 0.9993619 13 9 ESTs R01234 0.9993626 14 7 3'-phosphoadenosine 5'-phosphosulfate sy R99935 0.9993629 1 12 beta-catenin-interacting protein ICAT R78539 | R784 0.9993630 6 12 transcription termination factor-like pr AA620357 0.9993611 3 6 lymphocyte-specific protein tyrosine kin AA469965 1.0003214 1 5 UNK AA463452 1.0003219 3 7 fibroblast growth factor receptor 1 (fms AA281064 1.0003228 14 7 KIAA1268 protein T64956 1.0003228 10 13 EMPTY EMPTY 1.0003229 5 4 major histocompatibility complex, class AA458472 1.0003229 7 8 low molecular mass ubiquinone-binding pr T67012 1.0003231 4 7 Homo sapiens cDNA: FLJ23020 fis, clone L T67278 1.000326 1 4 GDP dissociation inhibitor 1 AA488681 1.001287 9 10 ESTs R26789 1.00128

25 12 2 KIAA0260 protein N80922 1.0012825 14 12 ESTs N74229 1.0012826 12 3 glutathione S-transferase M3 (brain) R63065 1.0012819 3 6 parathymosin R11526 1.0022422 4 2 2.19 gene R87497 1.002248 3 4 death associated transcription factor 1 AA425854 1.0032

19 4 13 EMPTY EMPTY 1.003232 14 4 thrombospondin 4 AA423957 1.00323 2 4 UNK AA490281 1.004163 6 12 phospholipase C, beta 4 W49563 1.00416

15 1 8 ESTs T97318 1.0041615 1 12 ESTs R16769 1.0041619 13 13 EMPTY EMPTY 1.0041627 1 11 EST N76018 1.0041628 8 10 proteolipid protein (Pelizaeus-Merzbache N49922 1.0041630 9 2 protein kinase C-like 2 AA098980 1.004163 13 13 EMPTY EMPTY 1.00512


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12 2 11 DKFZP434A043 protein H73237 1.0051219 1 8 EST R01712 1.0051220 6 10 ESTs N52234 1.0051215 7 13 EMPTY EMPTY 1.0060818 14 9 EST R21987 1.0060821 5 8 ESTs, Weakly similar to AF257182 1 G-pro T66828 1.0060822 1 13 EMPTY EMPTY 1.0060825 10 5 karyopherin (importin) beta 1 AA251527 1.0060810 5 10 ESTs N38984 1.0070421 5 5 autoantigen AA481276 1.0070425 10 13 EMPTY EMPTY 1.0070427 11 10 CGI-109 protein R53891 1.0070414 5 6 KIAA0606 protein; SCN Circadian Oscillat H48501 1.00818 7 10 ESTs R85839 1.00820 14 8 ESTs T90871 1.00821 13 12 NPC1 (Niemann-Pick disease, type C1, gen N53757 1.00822 5 12 ESTs N24581 1.0082 2 1 solute carrier family 14 (urea transport H82236 1.008962 8 9 ESTs R93087 1.008967 8 11 ESTs, Moderately similar to AF119917 62 N72574 1.00896

15 4 12 ESTs N38993 1.0089626 3 2 fibroblast growth factor receptor 2 (bac AA443093 1.0089627 5 7 ESTs, Highly similar to ALU6_HUMAN ALU S T90360 1.0089620 12 2 TATA box binding protein (TBP)-associate AA235706 1.0099226 2 13 EMPTY EMPTY 1.009922 1 12 heat shock protein hsp70-related protein AA620346 1.010887 4 3 carbonic anhydrase I R93176 1.010888 13 6 presenilin 1 (Alzheimer disease 3) AA411814 1.01088

12 9 13 EMPTY EMPTY 1.0108814 7 9 ESTs R06453 1.0108824 7 9 Human DNA sequence from clone RP1-18C9 o R06840 1.0108824 1 13 EMPTY EMPTY 1.010887 12 13 EMPTY EMPTY 1.01184

26 3 9 ESTs N70354 1.011846 7 10 ESTs N70988 1.0128

12 7 1 hydroxymethylbilane synthase R06263 1.012814 3 4 prostate differentiation factor AA450062 1.012820 11 13 EMPTY EMPTY 1.01282 3 7 serine (or cysteine) proteinase inhibito R54665 1.013763 8 7 keratin 18 AA018229 1.013765 11 3 ubiquitin-conjugating enzyme E2D 3 (homo AA017200 1.01376

11 8 11 UNK H64605 1.0137627 13 4 autocrine motility factor receptor AA479090 1.013767 2 3 ESTs, Highly similar to KFHU1 coagulatio N52835 1.01472

12 6 7 Homo sapiens mRNA; cDNA DKFZp761J17121 ( N52772 1.0147215 1 13 EMPTY EMPTY 1.0147229 10 6 cadherin 3, type 1, P-cadherin (placenta AA425217 1.0147230 3 1 phosphoinositide-3-kinase, catalytic, ga AA464176 1.0147230 13 8 Homo sapiens cDNA FLJ13207 fis, clone NT N80161 1.014728 6 8 ESTs R85439 1.01568

12 3 2 eukaryotic translation elongation factor R43766 1.0156816 1 7 ESTs T81312 1.01568


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22 10 10 ESTs R66269 1.0156824 8 1 v-myc avian myelocytomatosis viral relat R52824 | R664 1.0156825 7 10 hypothetical protein H59141 1.0156828 14 11 ESTs H94502 1.0156831 3 10 UNK R98107 1.0156831 9 11 ESTs N73604 1.015686 2 13 EMPTY EMPTY 1.016647 5 5 TATA box binding protein (TBP)-associate AA487148 1.016648 7 13 EMPTY EMPTY 1.016649 6 8 DKFZP586G0522 protein R78558 | R785 1.01664

20 13 8 ESTs N53965 1.0166423 10 10 reversion-inducing-cysteine-rich protein R26225 1.0166432 9 7 hypothetical protein FLJ10968 AA009624 1.0166412 1 13 EMPTY EMPTY 1.017618 10 3 glycogen synthase 2 (liver) N52282 1.017629 12 10 ESTs R27572 1.017630 14 10 ESTs R70141 1.01764 7 6 heterogeneous nuclear ribonucleoprotein R02069 1.018565 13 7 ESTs, Weakly similar to I38428 T-complex H94895 1.01856

23 14 1 solute carrier family 2 (facilitated glu R00833 1.0185618 3 3 CGI-28 protein AA490981 1.0195219 7 4 ESTs AA047812 1.0195232 5 5 dual-specificity tyrosine-(Y)-phosphoryl AA452149 1.019524 8 9 ESTs R16823 1.02048

11 2 9 spinocerebellar ataxia 7 (olivopontocere R63241 1.0204824 13 13 EMPTY EMPTY 1.0204825 6 2 msh (Drosophila) homeo box homolog 2 AA195636 1.0204830 8 6 ESTs, Weakly similar to no similarities T87139 1.020489 14 12 DKFZP566C134 protein N74357 1.02144

15 5 10 ESTs R98678 1.0214419 12 5 TNF receptor-associated factor 2 T55353 1.0214424 9 9 Homo sapiens mRNA full length insert cDN R07314 1.021447 2 13 EMPTY EMPTY 1.0224

10 11 8 ESTs T96523 1.022410 8 10 EST N49902 1.022415 11 12 hypothetical protein from EUROIMAGE 1759 R78580 | R785 1.022416 3 2 immediate early response 3 AA457705 1.022422 7 12 ESTs, Weakly similar to AT1A_HUMAN POTEN R86333 1.022416 9 9 ESTs R07320 1.0233626 3 1 ESTs, Highly similar to 1312232A kininog H69834 1.0233632 2 13 EMPTY EMPTY 1.0233610 14 11 EST H93482 1.0243214 7 8 hypothetical protein FLJ10761 H77536 1.0243218 12 12 ESTs, Weakly similar to A53856 aryl-acyl AA122269 1.0243219 1 9 UNK R63068 1.0243224 10 10 UNK R68210 1.0243227 6 11 small nuclear ribonucleoprotein polypept R83853 1.0243214 3 5 heme oxygenase (decycling) 1 T71606 1.0252816 9 5 M-phase phosphoprotein 6 AA478525 1.0252818 9 8 ESTs R10759 1.0252828 8 9 solute carrier family 6 (neurotransmitte W15241 1.025288 1 1 Human clone 23839 mRNA sequence R37846 1.02624


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15 10 9 putative mitochondrial outer membrane pr T95805 1.0262424 4 6 ESTs, Weakly similar to S65824 reverse t H51255 1.0262425 3 11 ESTs, Weakly similar to ALU5_HUMAN ALU S R00689 1.026242 8 7 ESTs N55360 1.0272

11 1 8 ESTs, Weakly similar to transformation-r R01725 1.027211 7 10 ESTs H60869 1.027214 6 6 protein-O-mannosyltransferase 1 R37635 1.027214 7 7 ESTs, Highly similar to 1011297A transfe R94592 1.027219 6 3 UNK AA236141 1.027224 2 8 ESTs R19310 1.027226 9 6 KIAA1320 protein R22316 1.02723 12 7 ESTs H98620 1.028168 11 5 keratin 7 AA485959 1.02816

11 7 6 phosphatidic acid phosphatase type 2B T71976 1.0281613 12 5 pre-B-cell leukemia transcription factor T59641 1.0281615 5 7 UNK R07998 1.0281618 9 10 EST R83160 1.0281619 2 11 L-pipecolic acid oxidase H37827 1.0281627 6 2 KIAA0378 protein R66454 1.0281630 13 5 transmembrane 9 superfamily member 1 AA127685 1.028162 8 8 EST T90374 1.029124 2 7 hypothetical protein FLJ20619 H79148 1.029129 9 8 Human transcription unit PVT gene, exons N70361 1.02912

10 6 2 Human clone 23612 mRNA sequence H65449 1.0291210 8 6 disabled (Drosophila) homolog 1 R01682 1.0291214 5 9 ESTs N92873 1.0291215 14 7 hypothetical protein FLJ11307 N53133 1.0291226 10 3 glycophorin A (includes MN blood group) N70285 1.029127 2 2 phosphoglucomutase 5 H12279 | H122 1.03008

10 7 3 ketohexokinase (fructokinase) T61256 1.0300811 14 5 vesicle-associated membrane protein 2 (s H56595 1.0300819 2 10 UNK H55748 1.0300825 13 2 KIAA0250 gene product T90118 1.0300825 14 11 ESTs H66839 1.030088 12 5 Homo sapiens mRNA, exon 1, 2, 3, 4, clon AA431970 1.031049 12 4 breast cancer 1, early onset H90415 1.03104

10 12 4 ribosomal protein S6 kinase, 70kD, polyp AA425446 1.0310415 2 13 EMPTY EMPTY 1.0310426 10 8 ESTs, Weakly similar to ALU6_HUMAN ALU S T91057 1.0310428 10 4 ras homolog gene family, member E AA443302 1.031047 10 11 ESTs N73877 1.032

11 5 9 Homo sapiens cDNA: FLJ22115 fis, clone H N59422 1.03221 14 10 KIAA0615 gene product R28614 1.03229 14 9 ESTs T96886 1.0325 14 3 tumor necrosis factor, alpha-induced pro AA456314 1.032969 12 9 ESTs T99846 1.03296

13 14 11 ESTs R94727 1.0329614 13 9 ESTs R08912 1.0329623 7 11 uncharacterized hypothalamus protein HTM N69661 1.0329623 3 12 ESTs R67071 1.0329626 7 8 EST, Moderately similar to ALU6_HUMAN AL T82270 1.032963 5 3 ATPase, Na+/K+ transporting, beta 2 poly H14808 1.03392


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14 14 10 UNK R70319 1.0339219 4 12 Friend leukemia virus integration 1 N50806 1.0339219 5 13 EMPTY EMPTY 1.0339222 12 8 ESTs T78454 1.0339226 6 7 hypothetical protein FLJ10290 H61530 1.0339228 13 1 renal tumor antigen N62873 1.0339230 6 7 Ubc6p homolog R01227 1.0339232 1 2 sialyltransferase 4C (beta-galactosidase AA453813 1.0339212 11 9 mannosidase, alpha, class 1A, member 2 R21232 1.0348813 9 7 hypothetical protein W84558 1.0348815 8 6 carbamoyl-phosphate synthetase 1, mitoch T61078 1.0348817 12 8 adenosine A1 receptor H21045 1.0348818 13 12 synaptosomal-associated protein, 29kD N58239 1.0348823 1 13 EMPTY EMPTY 1.034881 10 3 p53 inducible protein H12044 1.03584

32 9 10 ESTs R66896 1.035847 6 2 glycogen synthase kinase 3 beta R93912 1.03688 1 2 ankyrin repeat-containing protein AA434117 1.0368

11 11 9 ESTs, Moderately similar to KIAA1276 pro R00167 1.036812 5 1 inositol polyphosphate-1-phosphatase H52141 1.036814 2 7 KIAA1170 protein H80507 1.036816 3 7 UDP-N-acteylglucosamine pyrophosphorylas N68465 1.036818 4 9 ESTs R95916 1.036824 7 12 calpain 7 W32751 1.036827 1 10 ESTs H54423 1.036832 8 3 H.sapiens polyA site DNA H48097 1.036821 7 1 ubiquitin-like 4 N64628 1.0377624 5 11 erythroid differentiation-related factor H94872 1.037763 14 5 sushi-repeat-containing protein, X chrom AA449715 1.038724 10 7 DEAD/H (Asp-Glu-Ala-Asp/His) box polypep AA402879 1.03872

23 9 9 UNK N70127 1.0387218 3 9 ESTs R89368 1.0396825 1 5 nuclear phosphoprotein similar to S. cer AA488619 1.0396829 13 7 prothymosin, alpha (gene sequence 28) R00047 1.039683 1 6 signal transducer and activator of trans AA280647 1.04064

11 9 10 TNF receptor-associated factor 3 H48096 1.0406415 12 2 transcription elongation factor A (SII), AA412500 1.0406427 6 9 ESTs R68493 1.0406428 8 8 ESTs H78003 1.0406411 7 11 prion gene complex, downstream W73057 1.041612 13 9 ESTs R22212 1.04168 1 6 endothelin receptor type A AA450009 1.04256

16 6 13 EMPTY EMPTY 1.0425619 13 7 EST R64146 1.0425627 6 6 nucleosome assembly protein 1-like 3 AA463251 1.0425614 6 2 mesoderm development candidate 2 AA284495 1.0435229 7 2 RNA binding motif protein 3 AA054287 1.043528 6 7 succinate dehydrogenase complex, subunit H50345 1.04448

10 9 9 ESTs R31082 1.0454410 11 9 ESTs R77238 | R772 1.0454411 8 10 ESTs H69043 1.0454415 4 2 cell adhesion molecule with homology to R40400 1.04544


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22 5 4 cell membrane glycoprotein, 110000M(r) ( T46897 1.0454424 1 3 CD8 antigen, beta polypeptide 1 (p37) AA293671 1.0454426 14 1 sigma receptor (SR31747 binding protein W47485 1.045442 14 3 glia maturation factor, beta H22653 1.04645 12 13 EMPTY EMPTY 1.0464

14 10 2 transmembrane 4 superfamily member 4 H78244 1.046422 13 12 Homo sapiens cDNA: FLJ22071 fis, clone H R26827 1.046423 8 11 ESTs N71565 1.046426 9 9 inositol 1,4,5-triphosphate receptor, ty R68021 1.046427 5 11 very long-chain acyl-CoA synthetase; lip T95668 1.04641 6 6 flotillin 2 R73545 1.047369 10 12 ESTs H54384 1.04736

28 7 5 FYN oncogene related to SRC, FGR, YES H91826 1.0473615 7 11 ESTs, Moderately similar to ALU1_HUMAN A N69666 1.0483218 10 10 KIAA0966 protein N73551 1.0483220 10 8 holocytochrome c synthase (cytochrome c T89379 1.0483224 8 3 Rho GTPase activating protein 4 H70464 1.0483223 2 1 transducer of ERBB2, 1 AA490213 1.0492825 4 3 alcohol dehydrogenase 5 (class III), chi AA453776 1.0492831 12 1 splicing factor, arginine/serine-rich 2 AA454585 1.049282 13 8 KIAA1407 protein T97292 1.050244 8 5 E2F transcription factor 4, p107/p130-bi AA448641 1.05024

11 1 4 nuclear receptor coactivator 1 AA489785 1.0502412 9 9 Homo sapiens cDNA: FLJ23586 fis, clone L N71001 1.0502414 9 11 KIAA0453 protein R34372 1.0502418 3 7 lymphocyte-specific protein 1 R74169 1.0502423 5 10 Homo sapiens cDNA FLJ12776 fis, clone NT N73991 1.0502427 10 2 KIAA0276 protein AA410430 1.050244 3 10 ESTs, Weakly similar to B34087 hypotheti N34028 1.05125 4 4 small inducible cytokine A7 (monocyte ch AA040170 1.0512

17 5 7 zinc finger protein 207 N59119 1.051221 12 2 nuclear receptor subfamily 4, group A, m H37761 1.051229 13 13 EMPTY EMPTY 1.05124 5 13 EMPTY EMPTY 1.052167 9 4 dopachrome tautomerase (dopachrome delta AA478553 1.05216

11 8 7 dual-specificity tyrosine-(Y)-phosphoryl H62029 1.0521613 12 13 EMPTY EMPTY 1.052164 3 7 oxidoreductase UCPA R97517 1.05312

13 13 13 EMPTY EMPTY 1.0531223 6 11 ESTs N69476 1.0531226 13 11 ESTs, Moderately similar to ALU7_HUMAN A H90294 1.0531227 3 3 collagen, type XVI, alpha 1 R54968 1.0531232 10 13 EMPTY EMPTY 1.0531214 11 11 ESTs H62267 1.0540815 10 4 quinoid dihydropteridine reductase R38198 1.0540818 11 9 ESTs R17939 1.0540819 5 1 S100 calcium-binding protein P R32848 1.0540824 2 1 Homo sapiens cDNA: FLJ22488 fis, clone H R86843 1.0540825 12 13 EMPTY EMPTY 1.0540826 3 4 UNK AA454572 1.0540827 9 2 KIAA0329 gene product H75628 1.054082 7 3 interleukin 13 receptor, alpha 1 AA411324 1.05504


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6 3 8 oxysterol 7alpha-hydroxylase N64422 1.055048 4 9 hypothetical protein FLJ20036 H59114 1.05504

12 13 7 EST T97637 1.0550414 8 10 UNK H78783 1.0550431 1 9 KIAA0671 gene product R33011 1.0550431 6 13 EMPTY EMPTY 1.0550414 8 3 KIAA0054 gene product; Helicase T95553 1.05614 7 13 EMPTY EMPTY 1.05615 10 12 ESTs R33152 1.05621 4 12 Rho GTPase activating protein 5 N47967 1.05632 5 9 hypothetical protein PP2447 AA029368 1.0563 9 13 EMPTY EMPTY 1.05696

18 7 5 flavin containing monooxygenase 1 AA047666 1.0569620 9 8 UNK T83668 1.0569627 5 13 EMPTY EMPTY 1.056964 9 3 glycophorin B (includes Ss blood group) AA455338 1.057924 4 9 ESTs, Weakly similar to ALU1_HUMAN ALU S R92348 1.05792

17 6 13 EMPTY EMPTY 1.0579224 8 13 EMPTY EMPTY 1.057922 10 8 hypothetical protein FLJ11238 W58096 1.05888

11 4 13 EMPTY EMPTY 1.0588812 10 6 J domain containing protein 1 T95268 1.0588813 10 13 EMPTY EMPTY 1.0588822 2 7 ESTs, Weakly similar to ALU1_HUMAN ALU S H62823 1.0588822 13 8 ESTs T78644 1.0588826 2 9 ESTs R91821 1.0588828 5 10 ESTs R96206 1.0588830 4 1 peptidylglycine alpha-amidating monooxyg R66310 1.058885 13 6 ubiquitin-conjugating enzyme E2N (homolo AA490124 1.05984

14 6 9 ESTs H37832 1.0598422 9 2 Meis1 (mouse) homolog AA001444 1.0598422 3 11 leukemia inhibitory factor receptor N67017 1.0598425 6 7 ESTs N57750 1.0598426 6 2 UNK R01428 1.0598427 2 2 homeo box B5 H02243 1.0598417 7 11 UNK R06466 1.060818 2 4 SEC24 (S. cerevisiae) related gene famil T74758 1.060823 1 8 EST T97314 1.060823 6 10 EST H48467 1.06082 5 13 EMPTY EMPTY 1.061766 9 6 centrosomal protein 2 R87989 1.06176

10 9 6 amyotrophic lateral sclerosis 2 (juvenil R99314 1.0617612 13 12 ESTs R26700 1.0617620 14 3 fucosyltransferase 4 (alpha (1,3) fucosy R25419 1.0617620 6 4 neuroepithelial cell transforming gene 1 R24543 1.0617620 2 7 EST T86437 1.061764 4 13 EMPTY EMPTY 1.062726 9 8 ESTs H48470 1.06272

11 4 10 protein phosphatase 2 (formerly 2A), reg H57850 1.0627220 11 3 glucan (1,4-alpha-), branching enzyme 1 R09069 1.0627230 13 13 EMPTY EMPTY 1.062724 2 8 ESTs, Moderately similar to ALU8_HUMAN A R31105 1.06368


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4 4 8 ESTs R25114 1.063684 4 11 Homo sapiens mRNA; cDNA DKFZp667D095 (fr R26163 1.063685 14 10 ESTs R31512 1.063687 13 11 UNK R94610 1.06368

11 7 12 macrophage stimulating 1 receptor (c-met AA129089 1.0636811 8 13 EMPTY EMPTY 1.0636827 4 4 WW Domain-Containing Gene AA489498 1.0636828 4 2 spectrin, alpha, erythrocytic 1 (ellipto R07151 1.0636814 14 9 UNK R08883 1.0646416 3 6 Homo sapiens mRNA for KIAA1771 protein, N57773 1.0646422 14 11 EST H66442 1.0646425 13 7 ATPase, H+ transporting, lysosomal (vacu N20036 1.064646 1 8 ESTs R09498 1.06566 13 12 KIAA0993 protein R12477 1.06568 13 8 ESTs R02548 1.0656

10 5 7 Homo sapiens EST from clone 491476, full N63266 1.065610 11 10 ESTs R84376 1.065626 12 11 EST H89732 1.065627 1 1 KIAA0154 protein; ADP-ribosylation facto AA456041 1.06561 7 6 KIAA0300 protein AA405458 1.066568 14 9 ESTs R16733 1.06656

10 5 12 UNK W86376 1.0665613 8 13 EMPTY EMPTY 1.0665620 10 7 ESTs, Highly similar to C1HUQC complemen N69227 1.0665627 6 7 ESTs, Weakly similar to AF090895 1 PRO01 R16756 1.066562 4 13 EMPTY EMPTY 1.067524 6 6 ESTs, Weakly similar to KIAA1435 protein T97471 1.067524 10 8 tumor protein, translationally-controlle R09521 1.067526 12 5 myosin VIIA (Usher syndrome 1B (autosoma AA062993 1.06752

10 13 10 ESTs N67006 1.0675212 8 4 forkhead box J1 AA454609 1.0675218 14 10 ESTs H48124 1.0675220 6 5 fibroblast growth factor 2 (basic) R38539 1.0675231 4 2 nucleolar autoantigen (55kD) similar to W81191 1.067527 11 3 peptidyl-prolyl isomerase G (cyclophilin H14514 1.06848

18 8 1 interferon gamma receptor 1 H11482 1.0684819 3 11 ESTs N57713 1.0684820 5 13 EMPTY EMPTY 1.0684811 11 7 EST N69279 1.0694414 9 10 ESTs, Weakly similar to high cysteine ke R67568 1.0694414 8 13 EMPTY EMPTY 1.0694418 1 9 ESTs R88990 1.0694423 6 1 tropomyosin 1 (alpha) W58092 1.069443 13 9 ESTs R01245 1.07046 2 4 D123 gene product AA448289 1.07046 5 8 ESTs R08791 1.07047 13 4 cytochrome P450, subfamily IIC (mephenyt N53136 1.07048 9 12 KIAA0805 protein W46420 | AA2 1.0704

10 14 3 solute carrier family 2 (facilitated glu AA406552 1.070412 6 8 Finkel-Biskis-Reilly murine sarcoma viru T90293 1.070413 9 13 EMPTY EMPTY 1.070415 4 11 ESTs N64737 1.0704


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24 10 9 three-PDZ containing protein similar to R08117 1.070426 1 4 KIAA0088 protein H67274 1.07044 12 9 ESTs R21550 1.071368 11 13 EMPTY EMPTY 1.07136

10 14 9 ESTs R22034 1.0713610 8 11 hypothetical protein FLJ10697 W94105 1.0713611 1 6 Sp2 transcription factor T81103 1.0713611 2 13 EMPTY EMPTY 1.0713613 9 11 Homo sapiens protein kinase C-alpha mRNA N70440 1.0713625 3 13 EMPTY EMPTY 1.0713631 6 5 Human TB1 gene mRNA, 3' end H11501 1.071361 2 5 DR1-associated protein 1 (negative cofac AA406285 1.072324 5 6 KIAA1025 protein T72613 1.07232

27 9 12 UNK R36070 1.072321 12 1 ESTs, Moderately similar to ALU6_HUMAN A R00822 1.07328

13 9 10 ESTs R27314 1.0732813 1 13 EMPTY EMPTY 1.0732814 6 1 peptidylprolyl isomerase F (cyclophilin H05115 1.0732814 10 6 HP1-BP74 H79795 1.0732826 14 13 EMPTY EMPTY 1.0732831 10 9 ESTs, Moderately similar to ALU1_HUMAN A T95657 1.073281 12 8 MDS023 protein N52373 1.07424

10 10 11 ret finger protein 2 R07594 1.0742412 7 2 protein phosphatase 1, catalytic subunit AA443982 1.0742414 11 1 membrane metallo-endopeptidase (neutral R98936 1.0742414 13 8 ESTs N58198 1.0742420 13 7 Homo sapiens mRNA; cDNA DKFZp586I1420 (f R12405 1.0742422 3 8 ESTs, Moderately similar to NFY-C [H.sap W72005 1.0742412 13 13 EMPTY EMPTY 1.075218 8 8 ESTs T90561 1.075220 3 4 UNK AA405804 1.075226 1 11 Human DNA sequence from clone RP3-453C12 R67953 1.0761630 1 4 thiosulfate sulfurtransferase (rhodanese AA432063 1.0761632 10 5 protein tyrosine phosphatase, receptor t AA481547 1.076162 5 8 UNK T81972 1.07712

19 9 9 Homo sapiens cDNA: FLJ21449 fis, clone C R00480 1.077123 7 6 matrix metalloproteinase 16 (membrane-in H09997 1.078087 12 3 cyclin-dependent kinase inhibitor 2D (p1 R77517 1.07808

15 1 2 golgi autoantigen, golgin subfamily a, 2 AA424786 1.0780821 3 2 telomeric repeat binding factor (NIMA-in R33098 1.0780821 14 9 ESTs, Moderately similar to ALU6_HUMAN A T96909 1.0780824 5 8 ESTs T70541 1.0780830 12 12 ESTs, Weakly similar to SKD1_HUMAN SKD1 W04668 1.0780830 9 13 EMPTY EMPTY 1.0780810 5 8 ESTs R10917 1.0790414 7 11 Homo sapiens clone IMAGE:115304 mRNA seq N62660 1.0790418 8 9 ESTs R09053 1.0790423 13 6 copine I AA485922 1.079041 10 7 nuclear receptor coactivator 2 R77770 1.08

10 6 6 hypothetical protein FLJ11806 T66839 1.0815 3 1 vascular endothelial growth factor R45059 1.0815 8 8 glycoprotein M6B R42852 1.08


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15 4 10 UDP-glucose:glycoprotein glucosyltransfe R98442 1.0815 9 13 EMPTY EMPTY 1.0815 10 13 EMPTY EMPTY 1.0816 5 7 Homo sapiens, Similar to CG11985 gene pr R78559 | R785 1.0817 11 7 KIAA0618 gene product H81940 1.0819 1 1 UNK R44546 1.0822 12 11 ESTs H62897 1.0831 14 1 solute carrier family 2 (facilitated glu H38650 1.089 9 11 hypothetical protein FLJ11753 H96599 1.08096

10 4 13 EMPTY EMPTY 1.0809612 12 2 TAR (HIV) RNA-binding protein 2 AA436409 1.0809619 8 9 ESTs T98928 1.0809626 13 3 GRO2 oncogene R50407 1.080964 10 9 ESTs R20805 1.08192

10 11 2 adducin 1 (alpha) N53485 1.0819211 8 9 ESTs T99011 | T990 1.0819213 14 7 RAR (RAS like GTPASE) R33477 1.0819221 4 6 cytochrome P450, subfamily I (dioxin-ind AA448157 1.0819222 4 6 Homo sapiens mRNA; cDNA DKFZp586J1119 (f R98485 1.0819222 9 13 EMPTY EMPTY 1.0819228 2 9 DKFZP586P2219 protein R91083 1.0819231 7 13 EMPTY EMPTY 1.081925 14 4 DNA (cytosine-5-)-methyltransferase 1 N34857 1.08288

14 4 1 paraoxonase 1 R12373 1.0828820 2 2 fibrinogen, B beta polypeptide H91714 1.0828826 12 10 ESTs H47383 1.082883 8 8 ESTs R07619 1.083848 12 11 ESTs H65511 1.08384

11 5 1 paired-like homeodomain transcription fa T64905 1.0838417 9 7 hypothetical protein FLJ10783 W73797 1.0838418 13 10 Homo sapiens genomic DNA, chromosome 21q H47627 1.0838421 7 5 small inducible cytokine subfamily E, me AA504333 1.0838426 12 13 EMPTY EMPTY 1.0838428 1 2 fragile histidine triad gene AA256123 1.083849 14 4 UDP-N-acetyl-alpha-D-galactosamine:(N-ac R05278 1.0848

19 9 7 ESTs H91255 1.084829 9 1 transcription factor 3 (E2A immunoglobul AA026644 1.08481 2 13 EMPTY EMPTY 1.085761 11 13 EMPTY EMPTY 1.085766 13 4 tumor protein p53 (Li-Fraumeni syndrome) R39356 1.08576

14 7 10 ESTs H79046 1.0857615 4 9 ESTs R69244 | R69 1.0857632 2 10 interleukin 14 N34055 1.0857619 3 10 ESTs N62481 1.0867226 13 13 EMPTY EMPTY 1.086722 10 12 Homo sapiens RAB39 (RAB39) mRNA, complet AA485449 1.087686 8 10 ESTs H78825 1.087689 13 1 properdin P factor, complement H52256 1.087689 13 13 EMPTY EMPTY 1.08768

10 9 2 v-yes-1 Yamaguchi sarcoma viral oncogene R25397 1.0876811 1 9 ESTs R63090 1.0876811 3 11 ESTs R98027 1.08768


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14 10 10 mannosyl (alpha-1,3-)-glycoprotein beta- R66290 1.0876820 3 13 EMPTY EMPTY 1.0876822 9 12 hypothetical protein R94456 1.0876811 8 2 KIAA0303 protein AA418846 1.0886413 2 6 DnaJ (Hsp40) homolog, subfamily B, membe AA481758 1.0886416 9 1 matrix metalloproteinase 12 (macrophage R92994 1.0886418 14 8 UNK N74635 1.0886419 14 8 ESTs N28259 1.0886420 4 13 EMPTY EMPTY 1.0886422 7 4 KIAA0264 protein AA485919 1.0886430 5 10 Homo sapiens mRNA; cDNA DKFZp434G0614 (f H71854 1.0886431 10 1 tubulin, beta polypeptide H38210 1.088648 14 5 ornithine decarboxylase antizyme 1 AA487466 1.0896

15 5 11 ESTs, Weakly similar to M17 protein [M.m N69118 1.08965 3 5 protein predicted by clone 23627 W95389 1.09056

11 9 2 KIAA0280 protein AA425885 1.0905630 12 2 RAB interacting factor AA012882 1.090565 9 10 Homo sapiens cDNA: FLJ22807 fis, clone K R26854 1.091528 8 7 hypothetical protein FLJ20510 N53214 1.09152

14 12 2 Homo sapiens similar to son of sevenless R78735 1.0915227 8 13 EMPTY EMPTY 1.0915229 13 5 UNK AA490267 1.0915211 9 11 ESTs R85819 1.0924815 1 6 polymerase (DNA-directed), alpha (70kD) AA486289 1.0924820 3 1 Homo sapiens integrin, beta 1 (fibronect W67174 1.0924826 9 13 EMPTY EMPTY 1.0924810 6 11 ESTs, Weakly similar to ALU1_HUMAN ALU S N63786 1.0934415 4 6 complement component 7 AA598478 1.093446 1 13 EMPTY EMPTY 1.0944

15 9 1 transmembrane 4 superfamily member 3 AA045699 1.094416 9 10 ESTs AA034501 1.094420 6 6 H.sapiens novel gene from PAC 117P20, ch N74574 1.094422 12 7 nectin 3; DKFZP566B0846 protein R62781 1.094426 8 3 mannosidase, alpha, class 1A, member 1 T91261 1.09446 9 4 zinc finger protein 35 (clone HF.10) N64607 1.09536

10 12 10 ESTs H47542 1.0953615 2 4 mannose receptor, C type 1 H16389 1.0953620 10 10 Homo sapiens mRNA full length insert cDN N69453 1.0953629 7 1 EST T50788 1.095361 14 2 mastermind (Drosophila), homolog of R37148 1.096321 10 8 UNK R93237 1.096326 12 6 ESTs, Highly similar to T42691 hypotheti N69484 1.09632

19 7 9 RNA-binding protein gene with multiple s T98807 1.0963225 14 10 DNA (cytosine-5-)-methyltransferase 3 al H53133 1.0963231 3 7 ESTs, Weakly similar to ALU6_HUMAN ALU S T85249 1.096327 10 13 EMPTY EMPTY 1.09728

13 11 13 EMPTY EMPTY 1.0972820 7 3 Dombrock blood group N70349 1.0972829 7 6 cleavage stimulation factor, 3' pre-RNA, AA293218 1.0972831 3 12 EST H61003 1.097283 14 9 ESTs, Moderately similar to B54024 prote R01620 1.098243 5 11 RING1 and YY1 binding protein W72621 1.09824


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8 2 9 sec61 homolog H22136 1.098243 6 13 EMPTY EMPTY 1.09925 9 6 cathepsin S AA236164 1.0992

15 4 5 sarcoglycan, beta (43kD dystrophin-assoc R54807 1.099228 7 13 EMPTY EMPTY 1.099221 5 9 ESTs R34604 1.1001631 9 4 lectin, mannose-binding, 1 H74190 1.1001610 8 8 ESTs T90566 1.1011220 7 12 interferon regulatory factor 7 AA443090 1.1011226 5 10 Homo sapiens cDNA FLJ20153 fis, clone CO N95358 1.1011210 13 7 actinin, alpha 4 H51645 1.102081 12 12 UNK R71234 1.103042 12 1 splicing factor 3a, subunit 3, 60kD R43015 1.10304

12 10 8 ESTs N54901 1.1030424 2 4 testis-specific ankyrin motif containing AA432261 1.1030429 13 10 UNK R73868 1.103043 7 13 EMPTY EMPTY 1.104

11 2 12 v-myc avian myelocytomatosis viral oncog R83758 1.10417 5 13 EMPTY EMPTY 1.10430 3 9 ESTs H03493 1.10431 13 13 EMPTY EMPTY 1.10415 8 9 ESTs H77534 1.1049622 2 3 troponin T3, skeletal, fast AA449932 1.1049623 2 4 matrix metalloproteinase 16 (membrane-in H09997 1.104963 10 6 quinone oxidoreductase homolog AA668595 1.105928 4 10 ESTs H73896 1.105928 5 13 EMPTY EMPTY 1.10592

15 5 9 ESTs R75884 1.1059216 13 6 polymerase (RNA) II (DNA directed) polyp AA460830 1.105925 7 6 Misshapen/NIK-related kinase AA481868 1.10688

19 6 7 ESTs, Weakly similar to B28096 line-1 pr R08032 1.1068822 13 9 ESTs R69248 | R69 1.1068814 1 8 ESTs R09482 1.1078419 2 7 KIAA0630 protein N63644 1.1078421 6 1 ubiquitin protein ligase E3A (human papi R85213 1.1078431 13 7 nuclear mitotic apparatus protein 1 AA598659 1.1078412 6 9 neural polypyrimidine tract binding prot N64671 1.108819 6 13 EMPTY EMPTY 1.108829 14 7 solute carrier family 7 (cationic amino T98394 1.108830 10 13 EMPTY EMPTY 1.108810 8 13 EMPTY EMPTY 1.1097611 14 12 Homo sapiens cDNA: FLJ23220 fis, clone A N75947 1.1097629 5 11 ESTs, Weakly similar to T50633 hypotheti N70280 1.1097621 14 6 early endosome antigen 1, 162kD N66043 1.110721 12 13 EMPTY EMPTY 1.11168

12 3 13 EMPTY EMPTY 1.1116816 3 13 EMPTY EMPTY 1.1116824 12 2 nuclear receptor subfamily 1, group H, m H91456 1.111681 13 11 ESTs T80979 1.112646 6 12 Homo sapiens cDNA: FLJ23269 fis, clone C AA026686 1.11264

31 2 13 EMPTY EMPTY 1.1126432 14 7 NADH dehydrogenase (ubiquinone) flavopro W96224 1.11264


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14 3 11 TATA box binding protein (TBP)-associate N75438 1.113615 7 10 ESTs R99576 1.113632 2 5 glucose regulated protein, 58kD R33030 1.11365 13 13 EMPTY EMPTY 1.114566 12 8 Homo sapiens clone IMAGE:113431 mRNA seq T78487 1.114569 7 6 KIAA0323 protein H90765 1.11456

11 4 12 jun D proto-oncogene N66278 1.1145618 7 13 EMPTY EMPTY 1.1145621 7 13 EMPTY EMPTY 1.1145622 4 4 N-myc downstream regulated AA486403 1.114563 4 13 EMPTY EMPTY 1.115526 3 6 Friend leukemia virus integration 1 N50806 1.115527 10 8 Homo sapiens AKAP350C mRNA sequence, alt W56541 1.11552

12 5 2 ATPase, H+ transporting, lysosomal (vacu AA430654 1.1155218 9 2 UNK H65034 1.1155228 1 7 ESTs T91067 1.115523 4 9 ESTs R76551 | R762 1.116487 3 7 Homo sapiens cDNA: FLJ20895 fis, clone A N68404 1.116483 3 7 ESTs, Weakly similar to ALU4_HUMAN ALU S R92147 1.117448 9 4 v-myb avian myeloblastosis viral oncogen AA457034 1.11744

13 14 3 KIAA0704 protein R64600 1.1174419 3 8 ESTs R16764 1.1174424 9 11 HIV-1 inducer of short transcripts bindi R06252 1.1174412 9 11 Human DNA sequence from clone RP11-234G1 R10858 1.118414 2 13 EMPTY EMPTY 1.118416 3 8 maternally expressed 3 R11658 1.118428 5 9 dynein, cytoplasmic, intermediate polype N38963 1.118416 10 7 prostaglandin E receptor 4 (subtype EP4) AA019996 1.1203217 12 10 TRABID protein H60572 1.1203224 14 4 proteoglycan 4, (megakaryocyte stimulati AA280514 1.120325 4 6 cyclin H AA454146 1.12128

10 4 11 ESTs H73003 1.1212812 8 9 ESTs R16814 1.1212815 14 5 myeloid cell nuclear differentiation ant N29376 1.1212824 3 11 ESTs R98042 1.1212819 5 7 Homo sapiens clone FBD3 Cri-du-chat crit R93715 1.1222423 9 4 KDEL (Lys-Asp-Glu-Leu) endoplasmic retic H27912 1.1222428 12 1 sema domain, immunoglobulin domain (Ig), AA455145 1.1222411 14 7 bromodomain and PHD finger containing, 1 T98019 1.123216 2 1 hypothetical protein AA429483 1.123221 10 4 egf-like module containing, mucin-like, T66980 1.123230 11 4 vitamin D (1,25- dihydroxyvitamin D3) re AA485226 1.123211 12 4 B-cell CLL/lymphoma 6 (zinc finger prote R99926 1.1241614 4 10 ESTs H72290 1.1241623 4 7 ESTs, Weakly similar to ALU1_HUMAN ALU S T66830 1.1241630 14 7 p75NTR-associated cell death executor; o R63543 1.124164 1 13 EMPTY EMPTY 1.12512

21 14 8 chitobiase, di-N-acetyl- W35203 1.1251222 13 10 UNK R69886 1.1251227 13 5 tryptophan 2,3-dioxygenase T72398 1.1251228 8 4 Rab geranylgeranyltransferase, beta subu AA456028 1.1251211 14 9 ESTs R01608 1.12608


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27 5 9 ESTs R64403 1.1260828 5 1 insulin-like growth factor 1 (somatomedi AA456321 1.1260812 9 5 distal-less homeobox 4 R92495 1.1270424 1 4 golgi autoantigen, golgin subfamily b, m AA485974 1.1270425 7 4 protein phosphatase 1, regulatory (inhib AA460827 1.127041 10 13 EMPTY EMPTY 1.128

16 5 3 SWI/SNF related, matrix associated, acti AA496809 1.12822 3 10 ESTs W92258 1.12829 1 11 ESTs N54507 1.12816 6 6 MKP-1 like protein tyrosine phosphatase AA129677 1.1289619 9 10 ESTs R53616 1.1289623 2 6 3-hydroxybutyrate dehydrogenase (heart, T67057 1.1289626 8 10 ESTs N49895 1.1289630 3 12 UDP-Gal:betaGlcNAc beta 1,4- galactosylt W02073 | AA2 1.1289631 9 5 caspase 7, apoptosis-related cysteine pr T50675 1.128962 5 2 Tax interaction protein 1 AA434406 1.129928 5 5 phosphoenolpyruvate carboxykinase 2 (mit AA186901 1.12992

12 6 13 EMPTY EMPTY 1.1299216 6 4 KIAA0225 protein AA600190 1.1299217 2 4 tetratricopeptide repeat domain 1 AA291718 1.1299218 14 3 glutamate receptor, ionotropic, AMPA 1 H23267 1.1299218 12 10 ESTs R97185 1.129922 8 11 KIAA1718 protein N26802 1.13088

17 11 6 solute carrier family 2 (facilitated glu H58873 1.1308820 7 1 UNK N62462 1.1308831 6 11 ESTs N69364 1.130886 4 13 EMPTY EMPTY 1.13184

10 5 2 hypothetical protein R85562 1.1318415 11 5 UNK T63362 1.1318415 4 7 UNK T64994 1.1318416 11 13 EMPTY EMPTY 1.1318427 3 13 EMPTY EMPTY 1.1318428 11 10 ESTs R89061 1.131847 13 13 EMPTY EMPTY 1.13288 13 10 Homo sapiens cDNA FLJ12094 fis, clone HE H42896 | AI82 1.13289 11 13 EMPTY EMPTY 1.1328

10 14 12 Homo sapiens clone 24538 mRNA sequence R65759 1.132817 12 13 EMPTY EMPTY 1.132824 6 12 STAT induced STAT inhibitor-2 AA137031 1.132826 1 13 EMPTY EMPTY 1.132827 9 6 pericentriolar material 1 AA164440 1.13289 14 7 KIAA1691 protein N58487 1.13376

20 7 13 EMPTY EMPTY 1.1337616 12 8 ESTs R11499 1.1347223 10 2 KIAA1232 protein R44815 1.1347224 14 10 phospholipase C, gamma 1 (formerly subty R76690 1.1347227 1 6 EphA1 N90246 1.134728 8 8 ESTs R99307 1.13568

10 10 4 ribosomal protein S6 kinase, 90kD, polyp AA452574 1.1356817 14 2 Ste20-related serine/threonine kinase N89756 1.1356825 10 9 KIAA1052 protein T99337 1.1356820 3 12 tuftelin-interacting protein AA485750 1.13664


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2 11 5 SMC1 (structural maintenance of chromoso AA598887 1.13763 6 8 SH3 and PX domain-containing protein SH3 R69277 | R69 1.1376

16 2 2 UNK H93121 1.137616 9 4 v-raf murine sarcoma viral oncogene homo W88566 1.137625 5 9 ESTs R63837 1.137615 2 5 Human Chromosome 16 BAC clone CIT987SK-A AA431770 1.1385617 9 8 WD repeat domain 6 H93308 1.1385614 3 6 guanylate cyclase 1, soluble, beta 3 AA458785 1.1395215 5 1 v-akt murine thymoma viral oncogene homo W72696 1.139521 11 2 ring finger protein 10 H73586 1.14048

28 6 10 LIS1-interacting protein NUDEL; endoolig N51048 1.1404830 5 2 KIAA0125 gene product H65343 1.1404830 8 13 EMPTY EMPTY 1.140483 2 6 protein phosphatase 2 (formerly 2A), cat AA599092 1.141448 11 11 ESTs H65042 1.14144

14 2 3 eukaryotic translation initiation factor AA448301 1.1414418 5 9 ESTs H56088 1.1414419 11 2 UBX domain-containing 1 W79444 1.1414425 4 13 EMPTY EMPTY 1.1414427 14 3 HMG-box containing protein 1 R08932 1.1414427 5 8 DnaJ (Hsp40) homolog, subfamily B, membe AA084517 1.1414430 7 1 UNK R31938 1.141443 2 3 hypothetical protein FLJ20624 R92227 1.1424

11 8 8 ESTs T95858 1.142411 6 9 EST R65798 1.142425 14 1 carboxypeptidase B-like protein N54494 1.142410 9 3 microtubule-associated protein 7 R77252 | R772 1.1433614 13 11 ESTs H63866 1.1433621 9 1 nuclear factor (erythroid-derived 2)-lik AA496576 1.1433627 5 5 forkhead box O1A (rhabdomyosarcoma) AA194765 1.1433631 1 6 excision repair cross-complementing rode H20856 1.1433613 12 4 signal sequence receptor, alpha (translo AA099394 1.1443214 5 1 tumor necrosis factor, alpha-induced pro AA476272 1.1443223 8 4 interferon regulatory factor 1 AA478043 1.1443224 13 4 nuclear receptor subfamily 1, group D, m AA454168 1.1443230 7 11 UNK N51828 1.1443212 8 2 betaine-homocysteine methyltransferase T58896 1.1452812 13 8 ESTs R95112 1.1452816 11 4 ubiquitin-conjugating enzyme E2I (homolo AA487197 1.1452828 7 6 Homo sapiens cDNA: FLJ21587 fis, clone C R26164 1.145283 3 13 EMPTY EMPTY 1.146247 7 12 contactin 1 R27316 1.14624

18 5 12 BANP homolog, SMAR1 homolog N54244 1.1462422 14 13 EMPTY EMPTY 1.1462424 14 9 EST R11627 1.1462427 1 7 hypothetical protein FLJ20188 R32875 1.1462431 1 1 adaptor-related protein complex 3, mu 2 R40767 1.1462432 9 1 matrix metalloproteinase 7 (matrilysin, AA031514 1.146244 13 12 EST R83847 1.14726 10 13 EMPTY EMPTY 1.1472

15 5 6 colony stimulating factor 2 receptor, be AA279147 1.147219 8 8 ESTs T66942 1.1472


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27 1 13 EMPTY EMPTY 1.147215 12 8 UNK H71893 1.1481623 3 13 EMPTY EMPTY 1.1481626 4 8 ESTs R26094 1.1481631 4 8 Homo sapiens cDNA FLJ11752 fis, clone HE T98182 1.1481624 9 13 EMPTY EMPTY 1.1491214 9 9 ESTs R06738 1.150087 11 9 UNK T96036 1.151048 3 5 UNK AA490263 1.152

12 4 2 epidermal growth factor receptor pathway AA490223 1.15214 1 13 EMPTY EMPTY 1.15219 12 13 EMPTY EMPTY 1.15221 8 2 prostaglandin D2 synthase (21kD, brain) R59579 1.15227 8 7 DKFZP564A122 protein N57723 1.15210 6 12 ESTs, Weakly similar to ALU3_HUMAN ALU S N21103 1.1529610 12 12 ADP-ribosylation factor-like 7 AA485683 1.1529626 1 3 protein phosphatase 4 (formerly X), cata AA405562 1.1529612 5 8 ESTs T90072 1.1539217 8 4 UNK AA281932 1.1539223 1 12 KIAA1543 protein R02036 1.1539224 12 12 ESTs, Weakly similar to unknown [S.cerev AA054978 1.1539210 7 2 phosphatidylinositol binding clathrin as R59062 1.1548810 3 13 EMPTY EMPTY 1.1548815 8 1 transcription factor AP-4 (activating en AA284693 1.1548815 8 12 ESTs, Highly similar to AF161399 1 HSPC2 H72315 1.1548822 4 8 ESTs R11341 1.1548825 9 13 EMPTY EMPTY 1.1548829 14 13 EMPTY EMPTY 1.1548810 2 5 AU RNA-binding protein/enoyl-Coenzyme A AA448711 1.1558425 14 13 EMPTY EMPTY 1.1558430 11 5 UNK T70031 1.155842 1 2 GATA-binding protein 3 H72875 1.15683 13 10 ESTs R89051 1.1568

12 5 5 fibroblast growth factor receptor 4 AA446876 1.156818 14 13 EMPTY EMPTY 1.156821 3 13 EMPTY EMPTY 1.156829 5 13 EMPTY EMPTY 1.15689 14 8 ESTs, Weakly similar to ALU8_HUMAN ALU S N68719 1.15776

14 13 4 tyrosinase (oculocutaneous albinism IA) N31933 1.1577626 9 10 ESTs R93206 1.1577630 3 6 forkhead box G1B R44020 1.157764 13 9 EST R22217 1.158727 1 9 ESTs R33116 1.158728 9 10 ESTs R67992 1.15872

14 7 1 carcinoembryonic antigen-related cell ad AA054073 1.1587214 14 13 EMPTY EMPTY 1.1587224 1 7 ESTs T91233 1.1587230 14 11 ESTs N63864 1.158727 3 1 vascular cell adhesion molecule 1 H07072 1.159687 10 12 DKFZP434B203 protein R67544 1.159687 8 13 EMPTY EMPTY 1.15968

15 11 7 KIAA0973 protein AA479623 1.15968


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18 5 5 A kinase (PRKA) anchor protein (gravin) AA478543 1.1596819 9 13 EMPTY EMPTY 1.1596822 2 8 ESTs R10671 1.1596830 7 12 neuralized (Drosophila)-like N30706 1.1596811 7 5 requiem, apoptosis response zinc finger AA496782 1.1606420 6 13 EMPTY EMPTY 1.1606421 10 13 EMPTY EMPTY 1.1606422 2 5 heterogeneous nuclear ribonucleoprotein AA126911 1.1606423 4 12 Homo sapiens mRNA; cDNA DKFZp586J0323 (f H91294 1.1606424 1 1 opioid-binding protein/cell adhesion mol R38201 1.1606415 10 2 myeloid differentiation primary response H40681 1.161630 7 2 proteasome (prosome, macropain) 26S subu AA112486 1.16162 5 4 solute carrier family 35 (CMP-sialic aci AA460679 1.162565 5 13 EMPTY EMPTY 1.16256

14 9 8 ESTs T72671 1.1625616 11 1 v-myb avian myeloblastosis viral oncogen N49284 1.1625618 1 12 DKFZP434D156 protein AA447974 1.1625628 8 6 fer-1 (C.elegans)-like 3 (myoferlin) H26176 | H26 1.1625629 5 12 TGF beta receptor associated protein -1 H22171 1.1625632 2 2 ras homolog gene family, member H N90273 1.1625630 2 4 protein tyrosine phosphatase, receptor t H23202 1.1635232 6 8 ESTs R14859 1.163524 4 4 ubiquitin specific protease 13 (isopepti AA190583 1.164489 14 13 EMPTY EMPTY 1.16544

10 9 10 ESTs N49883 1.1654419 2 4 somatostatin R51912 1.1654419 2 13 EMPTY EMPTY 1.165448 8 13 EMPTY EMPTY 1.1664

14 10 13 EMPTY EMPTY 1.166422 3 6 glucokinase (hexokinase 4) regulatory pr T67006 1.1673624 9 4 UNK T50498 1.1673626 2 11 hypothetical protein FLJ22313 N71365 1.1673628 3 9 transcription factor BMAL2 R91259 1.1683210 13 12 acetyl-Coenzyme A transporter H66943 1.1692810 7 13 EMPTY EMPTY 1.1692811 11 2 KIAA0239 protein AA454740 1.1692811 2 8 ESTs R02728 1.1692832 8 2 ribosomal protein S6 kinase, 90kD, polyp H55921 1.169282 3 13 EMPTY EMPTY 1.170243 5 9 EST R66994 1.17024

13 10 7 myeloid leukemia factor 1 W56360 1.1702410 6 8 protein kinase Njmu-R1 T97215 1.171214 10 4 xeroderma pigmentosum, complementation g AA453300 1.171231 13 3 glycoprotein A33 (transmembrane) AA055811 | AI 1.17124 14 5 KIAA0063 gene product T58135 1.17216

10 1 8 Homo sapiens cDNA FLJ13522 fis, clone PL R22138 1.1721610 3 11 fibrinogen, B beta polypeptide H91470 1.1721627 5 3 UNK AA026609 1.172164 5 5 fibroblast growth factor receptor 2 (bac AA443093 1.17312

11 12 13 EMPTY EMPTY 1.1731218 13 5 KIAA0144 gene product N52646 1.1731218 9 9 G protein-coupled receptor, family C, gr W35153 1.17312


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22 7 9 ESTs R06370 1.1731222 11 11 ring finger protein 9 N68292 1.1731231 3 13 EMPTY EMPTY 1.173129 13 10 SRY (sex determining region Y)-box 22 R67223 1.17408

14 10 1 minichromosome maintenance deficient (S. AA456608 1.1740814 3 3 tissue factor pathway inhibitor 2 AA399473 1.1740819 10 13 EMPTY EMPTY 1.1740820 2 5 glutaredoxin (thioltransferase) AA291163 1.1740823 5 11 ESTs N68863 1.1740832 3 9 ESTs H24938 1.174081 2 4 ubiquitin specific protease 14 (tRNA-gua T65861 1.175046 10 11 ESTs R73647 1.17504

19 3 9 ESTs R63503 1.1750422 13 11 ESTs H63744 1.1750423 9 2 nuclear receptor subfamily 1, group H, m H61726 1.175044 7 2 caveolin 2 T89391 1.1766 5 1 MCF.2 cell line derived transforming seq H05800 1.1766 8 8 ESTs T72621 1.176

28 10 6 HSPC025 H63976 1.17629 12 13 EMPTY EMPTY 1.1768 1 13 EMPTY EMPTY 1.17696

12 2 10 deiodinase, iodothyronine, type II AA018182 1.1769619 6 2 KIAA0376 protein R43372 1.1769623 1 10 ESTs R96393 1.1769624 13 12 sperm associated antigen 9 N58144 1.1769625 2 4 thymidine kinase 2, mitochondrial AA029497 1.1769622 3 13 EMPTY EMPTY 1.1779225 7 13 EMPTY EMPTY 1.1779214 10 9 ESTs, Weakly similar to ALU1_HUMAN ALU S R16900 1.1788822 1 6 pre-alpha (globulin) inhibitor, H3 polyp T67884 1.1788830 3 10 ESTs H95342 1.178882 2 13 EMPTY EMPTY 1.17984

19 9 11 ESTs R06424 1.179842 9 13 EMPTY EMPTY 1.1808

11 10 13 EMPTY EMPTY 1.180823 11 1 UNK AA039370 1.18083 8 13 EMPTY EMPTY 1.18176

32 11 11 UNK H65481 1.181765 11 6 coatomer protein complex, subunit beta 2 T53792 1.182726 4 4 ATPase, H+ transporting, lysosomal (vacu AA487588 1.182727 6 8 EST T67285 1.18272

15 14 6 tumor protein p53-binding protein, 1 AA521389 1.1827227 3 11 ESTs N68442 1.1827231 11 12 CHMP1.5 protein R69236 | R69 1.182724 12 7 ribosomal protein S14 H73727 1.183687 6 11 ESTs N69578 1.183687 9 11 EST N73842 1.18368

15 2 9 ESTs R33422 1.1836820 1 11 bile acid Coenzyme A: amino acid N-acylt R98624 1.1836830 6 13 EMPTY EMPTY 1.183681 10 6 UNK AA452909 1.184645 8 2 tumor differentially expressed 1 H23255 1.18464


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18 11 8 ESTs R10334 1.1846420 13 5 Wilms' tumour 1-associating protein AA598802 1.1846430 4 4 stromal cell-derived factor 2 R45235 1.1846432 9 13 EMPTY EMPTY 1.1846412 13 1 ras association (RalGDS/AF-6) domain con R83224 1.185615 12 13 EMPTY EMPTY 1.185627 4 11 ESTs H52623 1.18565 8 13 EMPTY EMPTY 1.18656

19 7 8 CD86 antigen (CD28 antigen ligand 2, B7- H16746 1.1865623 10 12 ESTs R98676 1.1865623 7 13 EMPTY EMPTY 1.1865624 2 9 ESTs H38977 1.186565 14 12 ESTs N54488 1.18752

16 6 10 ESTs H79773 1.1875220 8 8 ESTs T84085 1.1875231 5 12 KIAA0129 gene product T83665 1.1884832 13 7 ESTs N59646 1.1884832 12 12 ESTs, Weakly similar to YZ28_HUMAN HYPOT AA046424 1.1884814 4 8 ESTs R14894 1.1894430 1 9 ESTs N59766 1.189441 11 1 protein tyrosine phosphatase, non-recept AA434420 1.19046 11 9 ESTs, Moderately similar to ALU4_HUMAN A R07646 1.19048 1 11 myeloperoxidase R05801 1.1904

10 11 11 Homo sapiens mRNA; cDNA DKFZp727C191 (fr N68257 1.190416 10 1 mannose-binding lectin (protein C) 2, so T69284 1.190416 10 2 heterochromatin-like protein 1 AA132226 1.190427 10 1 protein kinase, interferon-inducible dou AA010079 1.190421 4 13 EMPTY EMPTY 1.1913622 9 11 Homo sapiens cDNA: FLJ22930 fis, clone K R22335 1.1913624 3 10 ESTs H72700 1.191366 9 9 ESTs R06745 1.192329 12 2 KIAA0254 gene product N75979 1.19232

18 13 7 hypothetical protein FLJ10604 N72697 1.1923223 12 1 spinocerebellar ataxia 2 (olivopontocere R10604 1.1923227 11 2 tuberous sclerosis 1 (hamartin) AA455968 1.1923227 8 5 Homo sapiens germline mRNA sequence H16573 1.1923215 6 3 cytochrome P450, subfamily IIE (ethanol- H50500 1.1932819 5 9 Homo sapiens cDNA: FLJ21086 fis, clone C R64660 1.1932822 10 13 EMPTY EMPTY 1.1932826 3 8 ESTs R23190 1.1932829 9 6 cell division cycle 25A H59260 1.193283 2 7 CDC42-binding protein kinase beta (DMPK- N54476 1.19424

10 10 12 guanylate cyclase activator 1A (retina) AA431439 1.1942422 13 1 UNK H86755 1.194241 5 9 ESTs R63900 1.196163 12 10 hypothetical protein FLJ12888 N68390 1.197126 11 13 EMPTY EMPTY 1.19712

11 5 3 acid phosphatase 5, tartrate resistant R08817 1.1971213 3 6 topoisomerase (DNA) I AA232856 1.1971216 11 11 UNK H64973 1.1971222 12 1 mitochondrial ribosomal protein L3 H05820 1.1971225 1 1 SREBP CLEAVAGE-ACTIVATING PROTEIN R54823 1.19712


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10 9 13 EMPTY EMPTY 1.1980815 6 8 ESTs T67181 1.1980827 6 3 ATPase, H+ transporting, lysosomal (vacu H05768 1.1980827 1 4 JAK binding protein AA280137 1.1980831 14 12 ESTs N55272 1.1980832 1 13 EMPTY EMPTY 1.198088 14 12 Homo sapiens cDNA: FLJ22300 fis, clone H R06438 1.199042 3 9 ESTs N63808 1.2

11 6 6 DEAD/H (Asp-Glu-Ala-Asp/His) box polypep AA465387 1.211 9 13 EMPTY EMPTY 1.220 6 11 ESTs N79558 | AI66 1.221 1 13 EMPTY EMPTY 1.221 11 13 EMPTY EMPTY 1.222 7 13 EMPTY EMPTY 1.229 10 11 ESTs N71002 1.231 4 9 ESTs H77727 1.25 10 7 stromal cell-derived factor 2 R45235 1.200966 10 10 EST R66370 1.20096

14 6 4 KIAA0273 gene product AA405628 1.2019217 13 3 KIAA0914 gene product N51424 1.2019228 9 11 actin, beta AA031770 1.2019222 6 13 EMPTY EMPTY 1.2028826 8 13 EMPTY EMPTY 1.2028832 7 2 zinc finger protein 161 AA232647 1.202883 14 3 beclin 1 (coiled-coil, myosin-like BCL2- AA427906 1.203843 1 10 ESTs H54811 1.20384

22 14 9 ESTs R08866 1.2038426 14 10 EST H48115 1.203847 4 13 EMPTY EMPTY 1.2048

11 10 7 neuritin R66101 1.20483 8 10 ESTs H62167 1.20576

26 12 6 Wolf-Hirschhorn syndrome candidate 1-lik T97900 1.2057632 11 4 plasminogen activator, urokinase AA284669 1.205762 1 4 KIAA0082 protein AA504460 1.20672

19 14 11 ESTs N53578 1.2067219 8 12 wingless-type MMTV integration site fami AA434160 1.2067229 11 13 EMPTY EMPTY 1.206723 8 11 KIAA0781 protein H90161 1.20768

21 9 10 ESTs, Moderately similar to ALUC_HUMAN ! R68597 1.207686 10 6 KIAA0670 protein/acinus H75862 1.20864

11 14 6 KIAA1200 protein AA460986 1.2086415 8 7 Homo sapiens cDNA: FLJ23371 fis, clone H R16726 1.2086427 13 13 EMPTY EMPTY 1.2086432 9 8 ESTs T77758 1.2086432 7 12 ESTs T86959 1.2086426 10 13 EMPTY EMPTY 1.209632 5 1 pregnancy specific beta-1-glycoprotein 1 T95748 1.20964 13 4 protein tyrosine phosphatase, receptor-t AA476461 1.21056

12 1 7 P311 protein N55251 1.2105620 1 13 EMPTY EMPTY 1.2105622 12 13 EMPTY EMPTY 1.2105626 2 4 KIAA0080 protein AA429657 1.21056


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11 5 8 Homo sapiens clone CDABP0113 mRNA sequen H97765 1.2115216 10 11 transmembrane protein 2 H75632 1.2115223 4 13 EMPTY EMPTY 1.2115229 3 13 EMPTY EMPTY 1.2115230 6 1 phosphotidylinositol transfer protein R89808 1.2115231 12 11 GK001 protein R93507 1.211526 9 7 Homo sapiens HRBPiso mRNA, complete cds N92148 1.21248

16 2 4 AT-binding transcription factor 1 AA281616 1.2124816 5 13 EMPTY EMPTY 1.2124820 12 13 EMPTY EMPTY 1.2124822 4 11 KIAA0871 protein R74171 1.2124822 10 12 RNA cyclase homolog AA453591 1.212489 3 2 Bet1 (S. cerevisiae) homolog H54289 1.21344

16 1 8 EST R10301 1.2134421 2 13 EMPTY EMPTY 1.213442 8 13 EMPTY EMPTY 1.21445 14 6 survival of motor neuron 2, centromeric AA448194 1.2144

22 5 1 glucosamine-6-phosphate isomerase H48494 1.21448 6 13 EMPTY EMPTY 1.21536

11 14 1 phosphatidylinositol glycan, class F AA098979 1.2153631 10 7 LIM domain binding 2 H73914 1.215367 10 1 transforming growth factor, beta 1 R36467 1.21632

11 1 3 amphiphysin (Stiff-Mann syndrome with br H06483 1.2163218 10 1 receptor tyrosine kinase-like orphan rec N94921 1.2163218 12 3 glutamate-cysteine ligase, modifier subu W96179 1.2163220 13 13 EMPTY EMPTY 1.2163226 8 4 UNK T50313 1.2172830 14 12 ESTs N69207 1.2172814 6 13 EMPTY EMPTY 1.2182419 1 12 RAB3A, member RAS oncogene family H14231 1.2182420 14 7 ESTs, Weakly similar to DRPL_HUMAN ATROP R60807 1.2182421 8 13 EMPTY EMPTY 1.2182423 4 8 ESTs T98195 1.2182427 11 6 p53-induced protein H11660 1.2182428 11 13 EMPTY EMPTY 1.2182418 9 7 gamma-aminobutyric acid (GABA) A recepto W81526 1.219231 5 3 calpain 2, (m/II) large subunit AA102454 1.21924 8 2 cell division cycle 4-like AA041400 1.220166 11 10 Homo sapiens cDNA FLJ11216 fis, clone PL R67918 1.220167 6 9 paternally expressed 3 R68635 1.22016

11 5 7 ESTs R08298 1.2201612 8 1 fasciculation and elongation protein zet H20759 1.2201615 5 13 EMPTY EMPTY 1.2201631 9 13 EMPTY EMPTY 1.220163 10 13 EMPTY EMPTY 1.22112

12 11 6 UNK H81105 1.2211214 10 11 ESTs T87521 1.2211214 7 12 regulatory factor X-associated ankyrin-c AA459311 1.2211215 3 7 ESTs T97641 1.2211215 13 13 EMPTY EMPTY 1.2211223 6 6 collagen, type II, alpha 1 (primary oste N66737 1.221126 11 3 plakophilin 2 H66158 1.22208


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6 6 9 EST H38209 1.222087 7 11 hypothetical protein MGC861 N69694 1.22208

19 9 2 EST R69796 1.2220819 1 4 sulfotransferase family, cytosolic, 1C, W88655 1.2220831 5 6 complement component 1, s subcomponent T62048 1.2220831 4 13 EMPTY EMPTY 1.222081 11 6 UNK T41177 1.22304

18 11 5 lipin 1 AA446822 1.2230419 4 3 activating transcription factor 3 H21042 1.2230425 6 13 EMPTY EMPTY 1.2230430 6 9 ESTs H37798 1.223042 13 13 EMPTY EMPTY 1.224

12 10 13 EMPTY EMPTY 1.22420 8 13 EMPTY EMPTY 1.22423 9 12 ESTs R22952 1.22428 6 7 hepatocyte nuclear factor 3, gamma R99562 1.2246 3 7 interleukin enhancer binding factor 3, 9 AA449762 1.224967 3 3 clathrin, light polypeptide (Lca) AA113872 1.22496

20 8 6 hypothetical protein FLJ20701 H38799 1.2249621 14 1 synuclein, alpha (non A4 component of am AA455067 1.2249631 5 7 ESTs T90184 1.2249623 6 13 EMPTY EMPTY 1.2268828 10 11 ESTs R21759 1.226886 14 11 ESTs, Weakly similar to A46302 PTB-assoc N74350 1.227847 3 2 ATP-binding cassette, sub-family D (ALD) R02189 1.22784

11 8 6 milk fat globule-EGF factor 8 protein AA449667 1.2278412 3 10 UNK N33960 1.2278415 14 13 lipid transfer protein 4 Cont-4 1.2278422 14 10 ESTs R71391 1.227848 3 9 ESTs R93719 1.2288

12 8 8 ESTs N73883 1.228818 9 12 Homo sapiens mRNA for proacrosin binding AA443593 1.228811 7 13 EMPTY EMPTY 1.2297630 8 12 GK001 protein AA004671 1.229762 13 4 RAP1A, member of RAS oncogene family AA279804 1.230724 12 2 TAR DNA binding protein N69283 1.230726 13 7 oxysterol binding protein AA012822 1.23072

28 8 5 Rhesus blood group-associated glycoprote T48950 1.2307214 10 8 ESTs, Weakly similar to ALUC_HUMAN !!!! R07312 1.2316814 9 13 EMPTY EMPTY 1.2316815 3 12 ESTs H51056 1.2316811 4 1 serine hydroxymethyltransferase 1 (solub R52639 1.2326411 9 1 protein phosphatase 2 (formerly 2A), reg N63863 1.2326419 12 6 hepsin (transmembrane protease, serine 1 H62163 1.2326414 5 13 EMPTY EMPTY 1.233622 5 13 EMPTY EMPTY 1.23367 1 11 ESTs N57730 1.23456

23 5 7 ESTs, Weakly similar to ALU1_HUMAN ALU S N68871 1.2345631 14 4 cytochrome P450, subfamily IIA (phenobar T72259 1.2345631 3 6 interleukin 10 receptor, beta AA486393 1.234562 4 7 H-2K binding factor-2 R44578 1.23552

11 3 8 ESTs H78007 1.23552


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31 6 12 DKFZP434O125 protein H79706 1.2355215 14 9 Homo sapiens mRNA; cDNA DKFZp586H0324 (f R22788 1.2364816 2 13 EMPTY EMPTY 1.2364831 10 13 EMPTY EMPTY 1.2364812 8 11 ESTs T87109 1.237447 12 2 BTG family, member 3 N52496 1.23847 9 6 cadherin 5, type 2, VE-cadherin (vascula T53626 1.2384

15 7 4 interleukin 15 receptor, alpha AA054754 1.238417 1 13 EMPTY EMPTY 1.238430 3 2 nuclear cap binding protein subunit 2, 2 R37756 1.238431 12 5 karyopherin alpha 1 (importin alpha 5) AA180046 1.23848 12 1 lecithin-cholesterol acyltransferase R06458 1.23936

19 10 4 nuclear factor I/C (CCAAT-binding transc N20996 1.2393619 5 11 ESTs H95354 1.2393624 3 12 UNK W15574 1.2393630 4 13 EMPTY EMPTY 1.2393631 4 6 complement component 2 T71879 1.2393611 4 5 adenine phosphoribosyltransferase AA598510 1.2403211 11 12 protein kinase C, zeta R24258 1.2403211 12 7 sec22 homolog W47156 1.2412814 1 11 EST R69566 1.2412823 9 1 UNK H66070 1.2412823 9 6 UNK N39262 1.2412823 13 13 EMPTY EMPTY 1.2412828 1 3 proline-rich protein HaeIII subfamily 1 AA488051 1.241288 11 3 protein phosphatase 2, regulatory subuni R53787 1.242247 11 11 ESTs H75490 1.2432

14 6 10 EST H77479 1.243215 6 7 GW128 protein H62527 1.243223 4 3 UNK H91256 1.24324 5 1 inositol 1,4,5-trisphosphate 3-kinase B R94153 1.244164 8 1 TAT-INTERACTIVE PROTEIN, 72-KD AA055504 1.24416

17 2 6 v-ral simian leukemia viral oncogene hom W15297 1.2441622 10 9 RAB23, member RAS oncogene family W60718 1.2441619 7 13 EMPTY EMPTY 1.2451232 8 1 N-acetylgalactosaminidase, alpha- R46823 1.245125 10 11 ESTs N71438 1.24608

13 2 13 EMPTY EMPTY 1.2460823 6 7 sprouty (Drosophila) homolog 4 R70506 1.2460825 13 13 EMPTY EMPTY 1.2460830 11 3 purine-rich element binding protein A AA447793 1.246087 13 5 internexin neuronal intermediate filamen AA447618 1.247048 1 8 ESTs R10311 1.247048 7 8 ESTs N70680 1.24704

26 9 5 ephrin-B2 AA461108 1.2470419 12 10 ESTs, Weakly similar to ALU7_HUMAN ALU S N73834 1.24822 12 9 ESTs R07651 1.24812 2 8 ESTs R23952 1.2489615 4 1 v-myc avian myelocytomatosis viral oncog AA464600 1.2489619 3 13 EMPTY EMPTY 1.248968 8 10 ESTs, Weakly similar to AF227133 1 candi N52978 1.24992

18 6 3 phosphofructokinase, platelet AA608558 1.24992


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20 7 7 GTP-binding protein Sara W67309 1.250887 2 5 KIAA0430 gene product AA521490 1.25184

12 1 2 folate receptor 1 (adult) R24530 1.2518421 3 6 polymerase (RNA) II (DNA directed) polyp AA418689 1.2518425 5 2 phosphatidylinositol glycan, class B N47966 1.2518426 3 13 EMPTY EMPTY 1.251842 14 2 FYN-binding protein (FYB-120/130) N64862 1.2528

14 12 11 ESTs H63116 1.252826 6 8 ESTs T98549 1.252831 6 7 hypothetical protein, expressed in osteo N67034 1.25286 6 10 ESTs H77715 1.25376

11 12 1 plasminogen activator, urokinase recepto AA454879 1.2537619 2 8 ESTs R02599 1.2537630 3 8 ESTs R10702 1.253766 5 12 member of MYST family histone acetyl tra AA464974 1.25472

12 13 6 zinc finger protein 146 AA504351 1.2547215 9 3 thymine-DNA glycosylase AA496947 1.2547215 3 13 EMPTY EMPTY 1.2547226 11 11 ESTs H82232 1.254723 12 3 programmed cell death 10 R68555 1.25568

18 4 8 ESTs R32647 1.2556820 12 5 KIAA0118 protein AA076645 1.2556826 10 7 formin 2 R73539 1.255686 13 11 ESTs H64602 1.256647 11 13 EMPTY EMPTY 1.25664

25 9 10 ESTs R36138 1.256643 9 10 UNK R53021 | AI73 1.2576

11 5 13 EMPTY EMPTY 1.257612 10 4 ret finger protein H08725 1.257615 5 8 G protein-coupled receptor 86 T67105 1.257626 8 12 transcription factor Dp-1 N73611 1.257627 6 13 EMPTY EMPTY 1.25766 14 2 CASP2 and RIPK1 domain containing adapto R37937 1.25856

11 1 2 hypothetical protein FLJ13576 W35399 1.2585611 13 13 EMPTY EMPTY 1.2585612 13 11 ESTs H93040 1.2585617 7 13 EMPTY EMPTY 1.258569 6 5 nuclear matrix protein p84 AA281366 1.25952

16 4 13 EMPTY EMPTY 1.2595230 4 8 EST R11302 1.2595216 7 9 Homo sapiens HSPC076 mRNA, partial cds R06852 1.2604820 10 6 carbonic anhydrase XI N52089 1.2604832 12 5 Cbp/p300-interacting transactivator, wit AA432143 1.2604829 14 4 cold shock domain protein A AA455300 1.261447 8 8 ESTs R55750 1.2624

27 7 1 bromodomain-containing 2 H72918 1.262432 12 3 diubiquitin N33920 1.262411 13 12 ESTs N74458 1.2633613 14 1 sterol O-acyltransferase (acyl-Coenzyme R07296 1.2633620 3 6 hypothetical protein DKFZp762N0610 H54094 1.2633622 8 9 EST R06607 1.2633610 9 5 endothelin converting enzyme 1 AA279429 1.26432


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14 6 12 intermediate filament protein syncoilin W47135 | AA2 1.2643211 4 6 immunoglobulin lambda locus T67053 1.2662414 11 3 serine (or cysteine) proteinase inhibito AA115877 1.266242 9 7 hypothetical protein FLJ10441 AA027161 1.26727 6 13 EMPTY EMPTY 1.26729 1 12 UNK R70462 1.2672

12 6 1 IK cytokine, down-regulator of HLA II R39227 1.267223 11 4 diacylglycerol kinase, gamma (90kD) H05774 1.267223 10 13 EMPTY EMPTY 1.267218 12 9 I-kappa-B-interacting Ras-like protein 2 R63173 1.2681619 1 3 amyloid P component, serum R98019 1.2681617 10 5 aryl hydrocarbon receptor-interacting pr AA454926 1.2691217 11 13 EMPTY EMPTY 1.2691218 5 3 phospholipase A2, group IIA (platelets, T61271 1.269123 11 13 EMPTY EMPTY 1.27008

15 3 9 ESTs R33594 1.2700811 10 6 LPS-induced TNF-alpha factor AA625666 1.2710415 6 5 calcium and integrin binding protein (DN AA487575 1.2710416 7 3 heat shock transcription factor 2 AA253434 1.2710431 7 1 ESTs N79051 1.271044 2 13 EMPTY EMPTY 1.2729 11 12 ESTs N73510 1.272

28 2 1 mesenchyme homeo box 1 AA426312 1.2727 13 12 ESTs N55174 1.27296

11 7 7 UNK R01257 1.2729615 1 3 collagen, type XV, alpha 1 AA455157 1.2729624 2 2 Homo sapiens mRNA full length insert cDN AA083478 1.2729612 5 10 endoplasmic reticulum resident protein 5 N39195 1.2739226 2 1 CUG triplet repeat, RNA-binding protein R15111 1.2739230 14 5 translocation protein 1 AA450205 1.2739231 14 7 ESTs, Highly similar to S68178 mixed-lin T98616 1.273928 2 8 ESTs R19399 1.27488

15 11 9 metaxin 1 N69540 1.2748826 4 13 EMPTY EMPTY 1.2748828 2 13 EMPTY EMPTY 1.2758424 11 10 EST R68578 1.276817 14 1 UNK R73909 1.2777625 6 4 GTP binding protein 1 AA521469 1.2777626 6 1 inhibitor of DNA binding 2, dominant neg H82442 1.2777624 11 5 UNK N62179 1.278727 5 11 ESTs N69136 1.27968

16 8 3 mitogen inducible 2 AA490238 1.2796820 8 2 cryptochrome 1 (photolyase-like) R44018 1.2796826 5 8 ESTs R37496 1.2796830 5 13 EMPTY EMPTY 1.2796811 10 8 tankyrase 2 R62976 1.2806414 12 9 Homo sapiens mRNA full length insert cDN R07846 1.2806422 3 4 small nuclear ribonucleoprotein polypept AA253448 1.2806426 3 6 myeloid/lymphoid or mixed-lineage leukem N68458 1.2806411 2 6 RAB6A, member RAS oncogene family H20138 1.281616 4 12 KIAA0937 protein AA029444 1.2825618 11 2 mannosidase, alpha, class 2C, member 1 H45455 1.28256


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19 5 3 aconitase 2, mitochondrial H99699 1.2825627 11 4 UNK H61449 1.2825629 13 8 KIAA1067 protein H30094 1.2825610 14 13 EMPTY EMPTY 1.2835214 14 2 tumor necrosis factor receptor superfami W71984 1.2835210 6 13 EMPTY EMPTY 1.2844827 12 6 hexokinase 1 AA485272 1.2844831 1 13 EMPTY EMPTY 1.2844815 11 2 ring finger protein 4 H17943 1.2854418 11 13 EMPTY EMPTY 1.2854419 3 4 unc119 (C.elegans) homolog AA458807 1.2854427 14 6 solute carrier family 25 (mitochondrial AA411554 1.2854426 11 13 EMPTY EMPTY 1.286427 7 7 ret finger protein H08725 1.286429 14 1 Human T-cell receptor active alpha-chain AA427491 1.286430 10 1 MpV17 transgene, murine homolog, glomeru R55046 1.286416 8 13 EMPTY EMPTY 1.2873622 13 4 GW128 protein H62527 1.2883225 12 6 paraoxonase 3 R95740 1.2883226 11 4 CD59 antigen p18-20 (antigen identified H60549 1.2883228 4 6 KIAA1696 protein R02569 1.2883231 7 2 protein kinase C, eta AA047803 1.288327 9 12 ESTs H51275 1.289288 5 9 integrin, alpha 10 H43656 1.28928

23 5 9 ESTs R35663 1.2892831 10 3 hypothetical protein MGC10433 AA427717 1.289285 13 4 zinc finger protein 144 (Mel-18) AA464421 1.29024

18 13 8 Homo sapiens mRNA; cDNA DKFZp761G1823 (f T91035 1.2902410 12 13 EMPTY EMPTY 1.291212 2 5 glutamic-oxaloacetic transaminase 2, mit AA487521 1.291218 10 13 EMPTY EMPTY 1.291222 2 12 5-methyltetrahydrofolate-homocysteine me AA233650 1.291223 2 11 ESTs N62538 1.29128 7 3 UNK R70402 1.29216

14 8 1 upstream binding transcription factor, R N92443 1.2921615 9 10 UNK R26693 1.2921627 2 5 mitogen-activated protein kinase 14 AA404327 1.292162 4 2 UNK N91952 1.293126 5 13 EMPTY EMPTY 1.29312

12 14 5 KIAA0068 protein AA598583 1.2931227 7 4 osteoclast stimulating factor 1 AA283693 1.2931228 3 1 ESTs, Highly similar to IJHULM leukocyte W68403 1.2931212 5 13 EMPTY EMPTY 1.294084 3 4 1-acylglycerol-3-phosphate O-acyltransfe AA458922 1.29504

15 1 5 protein phosphatase 4, regulatory subuni T62952 1.2950417 9 10 Homo sapiens cDNA: FLJ21778 fis, clone H H93265 1.2950423 9 10 triadin R25665 1.295049 6 4 protein tyrosine phosphatase type IVA, m AA504327 1.296

11 3 2 structure specific recognition protein 1 R14855 1.29623 5 13 EMPTY EMPTY 1.29622 8 8 Homo sapiens clone IMAGE:110349 mRNA seq R10363 1.2969611 6 2 KIAA0372 gene product AA233339 1.29792


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3 9 9 ESTs R02597 1.2988826 8 2 carnitine palmitoyltransferase II W58236 1.298886 4 7 protein phosphatase 1, regulatory subuni T75485 1.299846 1 11 polymyositis/scleroderma autoantigen 1 ( AA458994 1.299846 7 13 EMPTY EMPTY 1.299849 2 13 EMPTY EMPTY 1.29984

14 5 7 coatomer protein complex, subunit alpha T81091 1.2998424 14 6 S164 protein AA446565 1.299841 8 12 v-myb avian myeloblastosis viral oncogen W86100 1.30082 14 6 poly(rC)-binding protein 2 AA431440 1.3008

14 10 12 hypothetical protein DKFZp566A1524 AA149105 1.300819 11 13 EMPTY EMPTY 1.300820 5 1 insulin-like 4 (placenta) R16073 1.30087 12 1 Sp3 transcription factor W31688 1.30176

12 8 10 ESTs N53167 1.3017623 10 1 trichohyalin AA258580 1.3017618 11 1 transformer-2 alpha (htra-2 alpha) R09691 1.3027225 12 8 ESTs R69333 | R693 1.3027210 7 11 hypothetical protein FLJ11939 N70072 1.3036811 4 9 Homo sapiens mRNA for KIAA1754 protein, R64153 1.3036820 12 6 hypothetical protein FLJ23017 H82948 1.3036822 14 12 UNK N58432 1.3036816 5 12 hypothetical protein FLJ21935 H73479 1.3046427 8 12 baculoviral IAP repeat-containing 1 AA482508 | AI 1.304645 14 2 proteasome (prosome, macropain) activato H65395 1.3056

14 14 1 lumican AA447781 1.305618 12 1 serine/threonine kinase 4 T95014 1.30568 7 11 ESTs T81107 1.30656

23 4 10 ESTs, Weakly similar to RST [M.musculus] H95360 1.3065626 8 11 ESTs W73366 1.306563 13 5 karyopherin (importin) beta 2 AA481067 1.307528 7 7 APMCF1 protein R95693 1.307529 10 1 tyrosine kinase 2 AA482128 1.30752

11 1 1 KIAA0146 protein AA401448 1.3075215 3 4 low density lipoprotein-related protein- AA486209 1.3075221 10 7 E2F transcription factor 4, p107/p130-bi AA448641 1.307521 6 4 reticulon 3 AA056089 1.308484 11 7 ATPase, Ca++ transporting, type 2C, memb N68998 1.30848

16 14 12 Homo sapiens mRNA; cDNA DKFZp586A191 (fr N71069 1.3084817 9 6 histone deacetylase 3 H79779 1.3084818 7 11 Homo sapiens mRNA; cDNA DKFZp434M0420 (f N69252 1.308482 1 1 t-complex-associated-testis-expressed 1- AA398011 1.309444 4 5 GTP cyclohydrolase 1 (dopa-responsive dy AA443688 1.30944

10 10 8 ESTs T90794 1.3094417 2 13 EMPTY EMPTY 1.3094418 5 11 ESTs N55520 1.3094424 12 13 EMPTY EMPTY 1.3094431 11 9 ESTs, Weakly similar to ALU1_HUMAN ALU S R06545 1.3094428 12 3 gap junction protein, beta 2, 26kD (conn AA490466 1.31044 3 11 ESTs R27734 1.311367 10 4 dihydrolipoamide dehydrogenase (E3 compo AA447748 1.311368 6 9 ESTs N72720 1.31136


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15 2 8 Homo sapiens clone IMAGE:121558 mRNA seq T97910 1.3113631 1 8 ESTs T97309 1.311362 2 4 KIAA0076 gene product AA477342 1.31232

11 11 13 EMPTY EMPTY 1.3123221 12 13 EMPTY EMPTY 1.3123227 8 8 Homo sapiens clone 23620 mRNA sequence R40970 1.312325 9 3 similar to vaccinia virus HindIII K4L OR H15747 1.313285 6 6 pro-platelet basic protein (includes pla W92812 1.31328

20 13 6 regulatory factor X-associated protein AA057436 1.3132830 1 8 ESTs R09153 1.313288 12 2 extracellular matrix protein 1 N79484 1.31424

24 9 1 matrix metalloproteinase 9 (gelatinase B T72581 1.3142427 7 9 Homo sapiens clone FLB6914 PRO1821 mRNA, N74244 1.314246 13 9 ESTs R27432 1.3152

11 6 11 Leman coiled-coil protein H93486 1.315226 6 13 EMPTY EMPTY 1.315227 9 8 Homo sapiens mRNA; cDNA DKFZp564H0764 (f R62566 1.315215 1 9 ESTs R33099 1.3161615 4 13 EMPTY EMPTY 1.3161616 13 7 NADH dehydrogenase (ubiquinone) 1 alpha AA022627 1.3161625 11 13 EMPTY EMPTY 1.3161611 13 7 KIAA0801 gene product H89698 1.3171211 12 10 GRB2-associated binding protein 1 H47315 1.3171225 3 2 chromodomain helicase DNA binding protei H99736 1.3171232 2 9 surfactant, pulmonary-associated protein N63892 1.317122 7 6 rec R09490 1.31808

14 13 13 EMPTY EMPTY 1.3180823 5 2 protocadherin 1 (cadherin-like 1) AA443558 1.3180828 4 4 lymphoid-restricted membrane protein AA457051 1.3180832 9 4 ATPase, H+ transporting, lysosomal (vacu AA486080 1.318088 2 5 peroxiredoxin 2 H68845 1.31904

14 2 4 for protein disulfide isomerase-related AA404387 1.3190424 3 13 EMPTY EMPTY 1.319042 5 9 ESTs N71488 1.325 14 8 non-kinase Cdc42 effector protein SPEC2 H48472 1.32

12 12 13 EMPTY EMPTY 1.3209615 6 13 EMPTY EMPTY 1.3209615 12 10 Homo sapiens mRNA; cDNA DKFZp564F112 (fr N28268 1.3219218 5 7 Homo sapiens mRNA; cDNA DKFZp564C2478 (f R78555 | R785 1.3219226 5 13 EMPTY EMPTY 1.3219231 12 4 excision repair cross-complementing rode N62586 1.3219218 11 10 ESTs R87642 1.3228825 3 12 UNK R60006 1.3228826 14 11 EST H93339 1.322887 6 1 tripeptidyl peptidase II R39682 1.32384

15 8 10 ESTs N59075 1.3238418 3 5 guanylate binding protein 1, interferon- AA486850 1.3238419 13 6 TATA element modulatory factor 1 AA252318 1.3238423 8 3 GRB2-associated binding protein 1 N68193 1.324828 5 5 fibulin 2 AA452840 1.3257632 3 2 purine-rich element binding protein A H46425 1.325763 12 2 pre-mRNA splicing factor similar to S. c AA454703 1.32672


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5 13 10 desmocollin 3 R28481 1.326729 2 1 ADP-ribosyltransferase (NAD+; poly (ADP- AA478959 1.32672

22 1 5 KIAA0973 protein AA479623 1.326722 14 11 ESTs N34057 1.327686 11 11 caveolin 2 H62838 1.32768

12 12 9 ESTs R26569 1.3276818 13 11 Homo sapiens HP43.8KD mRNA, complete cds N69220 1.327688 3 10 ESTs, Moderately similar to LAK-1 [H.sap H72977 1.328643 11 12 cytochrome P450, subfamily XIX (aromatiz R32428 1.32964 4 2 epidermal growth factor receptor (avian R35665 1.32964 8 6 hypothetical protein FLJ20580 AA456014 1.3296

16 2 3 surfactant, pulmonary-associated protein AA487267 1.329617 9 13 EMPTY EMPTY 1.329621 11 2 exostoses (multiple)-like 1 H19522 1.329627 8 6 solute carrier family 12, (potassium-chl AA166885 1.329614 4 9 ESTs H27541 | AI66 1.3305615 11 10 Homo sapiens cDNA FLJ11949 fis, clone HE R26892 1.3305622 5 3 small nuclear ribonucleoprotein polypept T54926 1.3305622 11 6 CDC14 (cell division cycle 14, S. cerevi N68854 1.3305624 1 8 hypothetical protein FLJ20345 R70925 1.3305618 10 9 ESTs R17017 1.3315221 13 13 EMPTY EMPTY 1.332485 7 13 EMPTY EMPTY 1.333448 1 7 ESTs R98045 1.3344

16 6 9 ESTs H63136 1.3353623 8 12 hypothetical protein FLJ22347 R16689 1.3353627 9 13 EMPTY EMPTY 1.335365 13 11 ESTs H21520 1.336325 11 13 EMPTY EMPTY 1.33632

20 12 11 ESTs H91057 1.3363231 12 10 ESTs R31533 1.3363214 12 3 acyl-Coenzyme A oxidase 1, palmitoyl H65660 1.337287 9 1 tumor necrosis factor, alpha-induced pro W92764 1.33824

19 3 12 v-raf murine sarcoma 3611 viral oncogene H59758 1.339228 13 5 anti-oxidant protein 2 (non-selenium glu AA598874 1.33924 12 6 Homo sapiens mRNA for FLJ00031 protein, R26125 1.34016

30 1 5 FSHD region gene 1 AA113339 1.340165 14 5 hypothetical protein R40649 1.341128 14 4 thioredoxin reductase 1 AA464849 1.341128 1 12 Homo sapiens mRNA; cDNA DKFZp564B1162 (f W90522 1.34112

26 14 2 SWI/SNF related, matrix associated, acti AA053810 1.341126 9 13 EMPTY EMPTY 1.34208

10 1 5 zinc finger protein 183 (RING finger, C3 AA132766 1.3420811 3 7 tumor necrosis factor alpha-inducible ce R70518 1.3420826 3 3 prostatic binding protein AA485909 1.3420832 14 5 proteasome (prosome, macropain) subunit, AA489343 1.342083 9 1 protein phosphatase 1, catalytic subunit R26186 | R264 1.343049 1 5 cullin 1 AA486790 1.34304

12 2 1 mitogen-activated protein kinase 10 R39221 1.343043 12 13 EMPTY EMPTY 1.3447 14 10 serum/glucocorticoid regulated kinase R27712 1.3447 7 13 EMPTY EMPTY 1.344


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30 8 10 ESTs N71371 1.34421 9 3 peroxisome biogenesis factor 13 R16849 1.3449629 14 10 KIAA1482 protein R28682 1.344965 10 1 thrombospondin 1 AA464532 1.34592

14 13 1 nuclear factor of kappa light polypeptid W56300 1.3459232 3 4 MCF.2 cell line derived transforming seq H28922 1.3459210 13 13 EMPTY EMPTY 1.3468817 8 1 restin (Reed-Steinberg cell-expressed in AA458868 1.3468824 10 13 EMPTY EMPTY 1.3468822 8 5 dynactin 1 (p150, Glued (Drosophila) hom AA488168 1.347845 5 2 translocase of outer mitochondrial membr AA457118 1.3488

12 3 12 ESTs, Weakly similar to BUTY_HUMAN BUTYR AA046498 1.348823 2 8 solute carrier family 2 (facilitated glu T97782 1.3497612 13 4 macrophage stimulating 1 receptor (c-met AA173454 1.3507219 14 4 arachidonate 5-lipoxygenase H51574 1.3507226 13 6 tryptophan rich basic protein AA404280 1.350722 2 11 choline/ethanolaminephosphotransferase H57959 1.35168

14 6 11 CGI-118 protein H73313 1.3516814 11 13 EMPTY EMPTY 1.3516824 12 3 menage a trois 1 (CAK assembly factor) AA481759 1.3516820 6 9 H2B histone family, member B N33927 1.3526426 7 2 carboxypeptidase D T56021 1.3526427 7 2 a disintegrin-like and metalloprotease ( AA398492 1.352648 4 13 EMPTY EMPTY 1.3536

18 13 4 mel transforming oncogene (derived from AA064668 1.353620 10 5 dihydrolipoamide S-succinyltransferase ( AA456824 1.353630 6 3 signal sequence receptor, alpha (translo AA450360 1.353615 10 10 KIAA0997 protein R26461 1.3545616 7 8 ESTs R07749 1.3545630 3 13 EMPTY EMPTY 1.3545614 13 7 pancreatic polypeptide 2 H67736 1.355528 3 1 protein phosphatase 1, regulatory (inhib AA428335 1.35648

23 13 2 solute carrier family 17 (sodium phospha N53056 1.3564831 8 9 Homo sapiens clone TCCCIA00427 mRNA sequ H95277 1.356484 5 2 MAD (mothers against decapentaplegic, Dr AA235597 1.35846 14 9 ESTs N65940 1.3584

23 3 9 KIAA0849 protein R33508 1.358431 5 13 EMPTY EMPTY 1.358419 6 1 replication factor C (activator 1) 1 (14 H73714 1.3593622 11 13 EMPTY EMPTY 1.3593626 13 12 Gene 33/Mig-6 H71881 1.359364 2 11 ESTs H75478 1.36032

18 3 13 EMPTY EMPTY 1.3603229 12 2 UNK H54686 1.3603219 10 1 protein kinase, cAMP-dependent, regulato AA485366 1.361283 14 13 chlorophyl a/b binding protien Cont-1 1.362244 1 9 ESTs R91060 1.36224

16 4 5 cell division cycle 25B H14343 1.3622425 12 9 POP7 (processing of precursor, S. cerevi H71218 1.362243 10 7 GrpE-like protein cochaperone H55907 1.3632

26 4 6 CGI-20 protein R02578 1.363220 2 8 EST R23924 1.36416


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20 14 12 ESTs W69216 1.3641621 13 5 ectonucleotide pyrophosphatase/phosphodi T69450 1.3641621 14 13 EMPTY EMPTY 1.3641615 5 12 netrin 4 R76614 1.365124 11 1 sulfite oxidase AA463642 1.366086 3 13 EMPTY EMPTY 1.36608

24 8 5 nucleoporin 88kD AA488609 1.3660826 1 2 gelsolin (amyloidosis, Finnish type) H72028 1.366089 12 3 eukaryotic translation initiation factor AA047039 1.36704

16 7 10 jumonji (mouse) homolog N73555 1.36818 2 6 PTPRF interacting protein, binding prote T66832 1.36819 1 2 FK506-binding protein 1B (12.6 kD) R08267 1.36820 7 6 ring finger protein 11 W94868 1.36824 6 6 integral membrane protein 3 AA034213 1.36815 13 10 UNK R31587 1.3699222 8 13 EMPTY EMPTY 1.3699221 12 5 UNK AA485677 1.3708829 6 12 UNK H75626 1.3708831 3 2 protein phosphatase 2, regulatory subuni R59165 1.371845 11 12 hypothetical protein FLJ22242 R36264 1.3728

10 5 5 tubulin, alpha, ubiquitous AA453471 1.372823 2 3 collagen, type IX, alpha 3 AA017526 1.372828 4 1 integrin, beta 5 AA434397 1.37288 2 3 interleukin 1 receptor-like 1 AA128153 1.37376

11 1 5 FBJ murine osteosarcoma viral oncogene h T61948 1.3737618 1 1 RAD50 (S. cerevisiae) homolog H99196 1.373768 6 12 hypothetical protein FLJ22344 R19299 1.37472

10 7 6 HBV pX associated protein-8 N69398 1.3747216 10 5 KIAA0758 protein R54359 1.3747222 12 6 hypothetical protein FLJ13910 N59542 1.3747211 2 5 DEAD/H (Asp-Glu-Ala-Asp/His) box polypep AA402879 1.3756818 4 7 kinesin family member 3A W70205 1.3766419 4 5 ATP-binding cassette, sub-family A (ABC1 AA054277 1.3766422 12 3 RNA 3'-terminal phosphate cyclase AA146803 1.376646 3 5 heat shock 10kD protein 1 (chaperonin 10 AA448396 1.3776

11 11 6 Tat-interacting protein (30kD) AA148230 1.377628 13 13 EMPTY EMPTY 1.377629 10 13 EMPTY EMPTY 1.37767 13 7 HSPC145 protein W32403 1.37952

15 11 13 EMPTY EMPTY 1.3795227 12 7 transcriptional intermediary factor 1 R38345 1.379522 3 4 related to the N terminus of tre AA281057 1.380483 14 10 ESTs H77627 1.38048

18 4 13 EMPTY EMPTY 1.3804822 13 7 peptidylprolyl isomerase A (cyclophilin H93021 1.380484 7 13 EMPTY EMPTY 1.38144

12 7 7 EST R16095 1.3814422 10 1 moesin R22977 1.3814425 5 13 EMPTY EMPTY 1.381447 9 13 EMPTY EMPTY 1.3824

20 5 8 ESTs N73838 1.382428 3 13 EMPTY EMPTY 1.3824


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32 11 3 interferon regulatory factor 3 AA456570 1.38243 1 13 EMPTY EMPTY 1.38336

10 11 13 EMPTY EMPTY 1.3833620 4 9 ESTs R92205 1.383365 14 13 EMPTY EMPTY 1.38432

10 12 5 suppressor of Ty (S.cerevisiae) 6 homolo R85545 1.3843217 13 5 step II splicing factor SLU7 T70057 1.3843231 2 4 membrane cofactor protein (CD46, trophob AA463497 1.3843231 9 8 ESTs, Highly similar to IRX3 MOUSE IROQU R55185 1.384323 9 12 ESTs, Weakly similar to KIAA0666 protein R78606 | R786 1.38528

11 5 2 Rho guanine exchange factor (GEF) 11 N54420 1.3852819 1 13 EMPTY EMPTY 1.385282 3 10 ESTs N45139 1.386243 2 13 EMPTY EMPTY 1.386244 6 7 hemoglobin, beta pseudogene 1 N55205 1.38624

11 13 9 ESTs R01235 1.3862420 7 5 phosphatidylinositol-4-phosphate 5-kinas AA482251 1.3862420 11 7 carbonic anhydrase III, muscle specific AA481780 1.3862430 9 9 Homo sapiens clone 022f05 My030 protein R06710 1.386247 11 4 Homo sapiens, Similar to deoxyguanosine R07506 1.38912

18 1 4 Homolog of yeast DNA-crosslink repair ge AA011189 1.389126 12 11 mouse double minute 4, human homolog of; N66001 1.390088 4 11 Homo sapiens clone IMAGE:110218 mRNA seq H72866 1.39008

14 11 2 growth factor, erv1 (S. cerevisiae)-like AA455303 1.3900819 11 1 ESTs R89715 1.3900827 3 4 retinoic acid- and interferon-inducible N98563 1.3900830 8 8 Homo sapiens cDNA FLJ11654 fis, clone HE N58251 1.3900832 7 10 Human DNA sequence from clone 167A19 on H90879 1.3900812 1 3 proliferating cell nuclear antigen AA450265 1.3910413 7 5 solute carrier family 16 (monocarboxylic AA425395 1.3910422 6 1 benzodiazapine receptor (peripheral) AA455945 1.391044 1 2 fms-related tyrosine kinase 1 (vascular AA058828 1.392

22 13 6 angio-associated, migratory cell protein AA452848 1.392967 1 13 EMPTY EMPTY 1.39392

26 8 1 guanylate binding protein 2, interferon- W72748 1.3939228 7 8 hypothetical protein MGC2652 R66088 1.393921 10 4 CD9 antigen (p24) AA412053 1.39488

12 14 6 low density lipoprotein receptor defect AA504455 1.394889 3 4 nuclear receptor co-repressor 2 T63031 1.395847 14 2 hypothetical protein FLJ20397 H16256 1.39688 1 9 ESTs N66839 1.3968

16 1 3 Homo sapiens, Similar to syntaxin 5A, cl AA452148 1.396827 9 1 protein tyrosine phosphatase, non-recept R06605 1.396820 5 10 ESTs, Weakly similar to S55024 nebulin, N38956 1.3977612 7 3 mitogen-activated protein kinase 1 W45690 1.398721 7 2 catenin (cadherin-associated protein), d AA025276 1.399681 3 6 proteoglycan 4, (megakaryocyte stimulati AA280514 1.399682 12 5 KIAA0160 protein AA521347 1.39968

18 5 2 Human clone 23695 mRNA sequence R44050 1.3996823 11 3 retinoblastoma-binding protein 8 H24301 1.399682 3 1 Kallmann syndrome 1 sequence H17883 1.40064

11 6 1 ras homolog gene family, member G (rho G R76314 1.4016


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13 11 10 ESTs, Weakly similar to T15138 hypotheti R68474 1.401616 14 10 Homo sapiens mRNA from chromosome 5q21-2 R77102 | R77 1.40166 9 12 glycine C-acetyltransferase (2-amino-3-k N93695 1.40256

10 6 4 similar to rat tricarboxylate carrier-li AA293453 1.4025626 4 4 seladin-1 AA482228 1.4025628 7 12 ESTs, Highly similar to AF279865 1 kines W02779 | AA2 1.4025630 4 11 hypothetical protein FLJ20185 R69267 | R69 1.403528 6 1 lipocalin 2 (oncogene 24p3) AA400973 1.40448

21 1 12 hypothetical protein FLJ10099 N52973 1.4044824 11 6 KRAB-zinc finger protein SZF1-1 R05934 1.4044823 7 4 interleukin 2 receptor, beta AA057204 1.4054410 7 5 seb4D AA459363 1.4073632 3 1 hypothetical protein PRO1847 R45056 1.4073617 13 13 EMPTY EMPTY 1.4083227 2 13 EMPTY EMPTY 1.4083228 6 13 EMPTY EMPTY 1.4083229 3 1 zinc finger protein, X-linked AA406372 1.408322 10 1 eukaryotic translation initiation factor W60015 1.40928

18 9 1 pleiomorphic adenoma gene 1 AA418251 1.4092822 13 2 glutathione transferase zeta 1 (maleylac AA428334 1.4092811 2 10 phosducin-like H55762 1.4102416 8 6 TATA element modulatory factor 1 AA252318 1.4102422 6 4 KIAA0274 gene product AA521198 1.4102428 6 2 suppressor of Ty (S.cerevisiae) 5 homolo R21511 1.4102413 3 13 EMPTY EMPTY 1.413126 8 13 EMPTY EMPTY 1.414088 9 2 zinc finger protein homologous to Zfp103 AA425020 1.41408

11 12 2 KIAA0229 protein N22435 1.4140813 13 5 UNK AA425352 1.4140830 2 12 islet cell autoantigen 1 (69kD) AA491302 1.414088 2 4 RAD23 (S. cerevisiae) homolog B AA489678 1.41504

22 11 5 glycoprotein M6B AA284329 1.4150418 12 13 EMPTY EMPTY 1.41625 8 12 v-jun avian sarcoma virus 17 oncogene ho W96155 1.416964 1 1 Putative prostate cancer tumor suppresso N66008 1.41888

14 5 2 gene predicted from cDNA with a complete H67667 1.4188818 6 11 ESTs, Weakly similar to T08663 hypotheti N63646 1.418888 5 11 ESTs, Weakly similar to T34352 hypotheti N59799 1.41984

17 14 13 EMPTY EMPTY 1.4198419 7 6 Human clone 23826 mRNA sequence AA172385 1.4198420 3 7 TJ6 protein N70122 1.4198429 10 1 UNK H80215 1.419847 11 8 MMS19 (MET18 S. cerevisiae)-like R78545 | R784 1.4208

18 1 3 UNK AA598795 1.420828 8 1 hyaluronan-mediated motility receptor (R R10285 1.420816 4 6 ESTs, Weakly similar to B28096 line-1 pr R92216 1.4217622 11 9 ESTs R07483 1.4217623 7 9 hypothetical protein FLJ11036 R22633 1.4217624 14 3 DNA segment on chromosome X (unique) 987 AA479062 1.4217624 3 6 KIAA0970 protein R36149 1.4227213 6 2 hypothetical protein H93328 1.4236832 5 7 sorting nexin 17 T91966 1.42368


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8 1 4 protease, cysteine, 1 (legumain) AA425938 1.4246412 6 5 collagen, type III, alpha 1 (Ehlers-Danl T98612 1.4246416 11 10 UNK R68586 1.4246419 6 9 UNK R65706 1.4246421 13 7 mitogen-activated protein kinase kinase H90855 1.424647 8 10 EST H48226 1.42656

10 11 3 glutathione S-transferase theta 2 AA490208 1.4265618 13 13 EMPTY EMPTY 1.4265627 6 4 amine oxidase, copper containing 3 (vasc AA036975 1.426563 1 9 RPB5-mediating protein R63092 1.42752

18 7 9 hypothetical protein MGC3123 R72507 1.4275226 10 4 ring finger protein 1 AA425772 1.4275229 1 13 EMPTY EMPTY 1.4275232 5 8 ESTs, Moderately similar to ALUC_HUMAN ! N52572 1.4275220 5 3 mitochondrial ribosomal protein L19 AA521243 1.4284825 1 13 EMPTY EMPTY 1.4284830 12 4 ubiquitin-conjugating enzyme E2B (RAD6 h AA598492 1.4284818 6 6 hypothetical protein FLJ11729 W15533 1.4294411 8 5 echinoderm microtubule-associated protei AA447196 1.430416 7 2 AE-binding protein 1 AA490462 1.4313620 12 1 sema domain, immunoglobulin domain (Ig), N50444 1.4313625 8 13 EMPTY EMPTY 1.4313631 7 6 cathepsin H AA487231 1.431367 8 2 UNK R45255 1.43232

10 7 9 Homo sapiens, clone MGC:13204, mRNA, com N62451 1.432321 9 3 phosphoserine phosphatase-like N75028 1.43328

20 14 5 lysyl-tRNA synthetase AA486220 1.4332825 11 6 Novel human gene mapping to chomosome 13 AA487590 1.4332818 11 7 zinc finger protein, subfamily 1A, 5 (Pe R11517 1.4342423 5 12 UNK H65286 1.4342411 6 7 ESTs H81275 1.435223 8 13 EMPTY EMPTY 1.435211 3 3 aldehyde dehydrogenase 3 family, member AA443630 1.4361612 11 11 ESTs H90531 1.4361615 7 1 transient receptor potential channel 1 AA017132 1.4361623 9 11 ESTs N73608 1.4361617 6 3 acyl-Coenzyme A dehydrogenase, very long AA464163 1.4371225 11 5 insulin-like growth factor binding prote AA478724 1.4371227 1 8 a disintegrin and metalloproteinase doma H79023 1.4371224 4 11 G protein-coupled receptor 48 H77855 1.4380826 13 4 retinoblastoma-binding protein 2 AA460157 1.4380832 9 12 regulator of G-protein signalling 5 N34362 1.438083 9 7 splicing factor 30, survival of motor ne H05112 1.443 13 12 ESTs N74626 1.447 5 3 hypothetical 43.2 Kd protein AA457092 1.447 8 5 glutamate receptor, ionotropic, N-methyl H08849 1.44

12 7 6 ESTs N71294 1.4417 10 13 EMPTY EMPTY 1.4418 2 13 EMPTY EMPTY 1.4419 13 4 spinocerebellar ataxia 1 (olivopontocere AA256508 1.4420 3 8 Homo sapiens cDNA FLJ13443 fis, clone PL R24224 1.4430 7 13 EMPTY EMPTY 1.44


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1 11 10 hypothetical protein FLJ10290 N69302 1.441927 12 5 nuclear antigen Sp100 AA447482 1.44192

16 1 4 dihydropyrimidinase-like 2 AA487460 1.4419228 14 1 actin filament associated protein R69356 | R693 1.441922 1 3 protein phosphatase 1, catalytic subunit AA443982 1.44288

17 7 9 ESTs T98244 1.4428822 12 2 son of sevenless (Drosophila) homolog 1 H64325 1.4428828 7 1 Homo sapiens mRNA; cDNA DKFZp586A181 (fr H44953 1.442881 12 2 nicastrin R96527 1.44384

22 5 11 phosphatidylinositol glycan, class F H80865 1.4438414 2 1 hypothetical protein FLJ23018 T83664 1.444823 8 1 transferrin H69531 1.444822 2 13 EMPTY EMPTY 1.4457626 5 1 interferon regulatory factor 2 AA416883 1.445764 11 4 Homo sapiens mRNA; cDNA DKFZp434I0812 (f AA479781 1.446726 6 13 EMPTY EMPTY 1.44672

27 8 4 nuclear factor I/B W87528 1.4467211 1 13 EMPTY EMPTY 1.4476815 11 1 general transcription factor IIF, polype AA479196 1.4476816 8 12 hypothetical protein FLJ22637 AA464143 1.4486418 2 5 death-associated protein AA458861 1.448644 9 7 hypothetical protein FLJ10416 similar to AA041406 1.4496

11 6 12 v-ral simian leukemia viral oncogene hom W15297 1.449632 7 11 EH-domain containing 3 R22327 1.449614 14 4 transforming growth factor, beta 3 AA040617 1.450564 9 12 fibulin 1 AA458882 1.45152

15 12 7 hypothetical protein 24432 H79142 1.451527 10 9 ESTs T95835 1.45248

29 2 13 EMPTY EMPTY 1.4524812 10 2 splicing factor, arginine/serine-rich 11 H56944 1.4534421 9 13 EMPTY EMPTY 1.4534422 14 8 ESTs T80608 1.4534417 4 1 signal recognition particle 19kD AA411407 1.454423 12 2 transcription factor 8 (represses interl H46554 1.454416 14 3 chromosome X open reading frame 12 AA455272 1.4553627 5 1 S-adenosylmethionine decarboxylase 1 R82300 1.455363 4 5 ubiquitin-conjugating enzyme E2D 3 (homo AA017200 1.456322 9 9 ESTs, Moderately similar to AF119917 63 N68173 1.45728

19 5 6 nicotinamide N-methyltransferase T72089 1.4572823 1 3 collagen, type XVIII, alpha 1 N81029 1.458249 2 4 Tax1 (human T-cell leukemia virus type I AA598483 1.46016

29 9 13 EMPTY EMPTY 1.4601616 8 11 hypothetical protein T98201 1.4611218 3 12 CGI-135 protein AA454862 1.461125 2 6 growth arrest and DNA-damage-inducible, AA147214 1.46208

21 11 3 E74-like factor 2 (ets domain transcript AA447783 1.4620811 3 9 Homo sapiens mRNA; cDNA DKFZp566D224 (fr R63528 1.4630420 13 2 serine/threonine kinase 3 (Ste20, yeast AA464529 1.4668829 8 13 EMPTY EMPTY 1.4668813 10 5 PDGFA associated protein 1 AA490300 1.468818 2 3 protein kinase (cAMP-dependent, catalyti AA281667 1.468824 8 10 EST, Highly similar to zinc finger prote H80869 1.47168


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30 1 11 proteoglycan 2, bone marrow (natural kil R67602 1.471683 14 11 ESTs H57135 1.47264

11 6 5 thyroid hormone receptor interactor 3 AA489011 1.4726423 7 6 UNK AA598950 1.4726430 8 11 ADP-ribosylation factor 6 AA012867 1.472648 11 1 ATP-binding cassette, sub-family C (CFTR AA424804 1.4736

14 12 13 EMPTY EMPTY 1.473618 12 8 ESTs T89283 1.473628 14 5 KIAA0071 protein AA457026 1.47368 13 12 ESTs R10719 1.47456

26 14 5 KIAA0097 gene product AA598942 1.4745616 14 4 thiosulfate sulfurtransferase (rhodanese AA461065 1.475522 11 4 suppression of tumorigenicity 13 (colon H65676 1.476489 7 5 SEC13 (S. cerevisiae)-like 1 AA496784 1.477449 6 9 Homo sapiens cDNA: FLJ21869 fis, clone H R63929 1.47744

23 9 5 hypoxia-inducible factor 1, alpha subuni AA598526 1.478427 12 13 EMPTY EMPTY 1.478430 2 7 guanine nucleotide binding protein (G pr T80856 1.47846 12 9 ESTs R07987 1.47936

24 7 6 EST R64131 1.4793614 8 9 UNK R06610 1.4803225 9 12 v-myc avian myelocytomatosis viral oncog W87741 1.4803216 12 11 ESTs H65338 1.4822420 9 4 ribonuclease, RNase A family, 1 (pancrea AA485893 1.482246 4 5 protein-O-mannosyltransferase 1 R37635 1.4832

32 8 5 pleckstrin homology-like domain, family AA258396 1.483212 9 8 Homo sapiens cDNA FLJ20812 fis, clone AD R08314 1.4841622 2 2 DiGeorge syndrome critical region gene 6 AA033564 1.484169 7 8 FK506-binding protein 5 W86653 1.48512

12 9 1 transcription factor AP-2 gamma (activat AA394236 1.4851225 3 6 uroporphyrinogen decarboxylase AA424344 1.4851226 2 7 aryl hydrocarbon receptor nuclear transl R14892 1.4860827 4 3 active BCR-related gene N99003 1.4860832 5 2 DAZ associated protein 2 R45111 1.486082 12 4 retinoblastoma-binding protein 4 AA428365 1.48704

10 5 4 zinc finger protein 161 AA232647 1.48830 1 13 EMPTY EMPTY 1.48823 3 10 ESTs R98192 1.489927 4 9 ESTs, Weakly similar to ALU1_HUMAN ALU S R35253 1.49088

16 13 13 EMPTY EMPTY 1.4908822 9 9 ESTs R06731 1.4908824 10 1 mannosidase, alpha, class 2A, member 1 H69561 1.4908816 7 4 KIAA0215 gene product H14805 1.492832 4 12 nephronophthisis 1 (juvenile) W44768 1.492816 1 13 EMPTY EMPTY 1.4937620 13 9 T-box 3 (ulnar mammary syndrome) R38300 1.4937621 12 9 Homo sapiens cDNA FLJ12381 fis, clone MA T95559 1.4937623 1 6 primase, polypeptide 1 (49kD) AA025937 1.4937626 3 10 hypothetical protein FLJ10330 N71647 1.4947213 2 5 hypothetical protein MGC5466 AA477261 1.4956818 5 10 uncharacterized bone marrow protein BM04 AA071470 1.4956810 9 12 Human DNA from chromosome 19-specific co W47419 | AA2 1.49664


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12 9 3 glycophorin E N38990 1.4966431 4 12 EST H90573 1.4966422 10 7 regulator of mitotic spindle assembly 1 R77718 1.49762 10 3 glycine dehydrogenase (decarboxylating; N58494 1.498563 13 4 asialoglycoprotein receptor 1 H58255 1.49856

23 13 5 ESTs, Highly similar to NOS3_HUMAN NITRI H01039 1.4985631 13 4 Homo sapiens cDNA FLJ13186 fis, clone NT N64794 1.5004831 12 13 EMPTY EMPTY 1.500488 8 9 DKFZP566K1924 protein R07024 1.50144

10 9 11 UNK AA121778 1.50243 9 6 Fc fragment of IgE, high affinity I, rec H79353 1.50336

11 5 12 EphA1 N90246 1.5052812 13 3 albumin H96241 1.5062425 6 5 tumor protein D52 AA459100 1.506245 14 11 ESTs R94840 1.50816

32 4 3 sialyltransferase 1 (beta-galactoside al AA598652 1.5091227 14 9 Homo sapiens mRNA; cDNA DKFZp761H1710 (f R01248 1.5100816 11 7 KIAA1482 protein N74897 1.5110419 13 11 calumenin H53025 1.5110410 1 4 KIAA0084 protein AA457047 1.51227 3 12 colony stimulating factor 1 receptor, fo R92609 1.5126 2 1 A kinase (PRKA) anchor protein 10 R21506 1.512966 2 12 acyl-Coenzyme A oxidase 2, branched chai T71782 1.51296

32 12 1 lipase, hepatic N68256 1.512966 13 13 EMPTY EMPTY 1.51392

21 8 5 Homo sapiens Ras suppressor protein 1 (R AA235332 1.5139220 14 1 GDP dissociation inhibitor 2 R92806 1.5148822 2 1 clones 23667 and 23775 zinc finger prote R40324 1.5148827 13 6 HS1 binding protein R76544 | R762 1.514884 4 10 lymphoid enhancer binding factor-1 W81129 1.51584

20 8 7 glycerol-3-phosphate dehydrogenase 1 (so AA193116 1.5158426 13 5 KIAA0152 gene product AA487921 1.5158427 12 8 APG5 (autophagy 5, S. cerevisiae)-like N95381 1.5158414 11 9 ESTs N80155 1.51687 12 9 ESTs T96148 1.51776

16 6 12 KIAA0202 protein N50853 1.5177629 6 5 proteasome (prosome, macropain) 26S subu AA450227 1.5177632 4 5 ATPase, H+ transporting, lysosomal (vacu AA430654 1.517768 3 7 ESTs H53038 1.51872

26 5 2 Homo sapiens mRNA; cDNA DKFZp586F1822 (f R99911 1.519686 12 4 ubiquitin-like 4 AA485397 1.52064

23 2 12 ESTs R24253 1.5206430 10 4 YY1 transcription factor AA491227 1.5225611 7 9 EST T98837 1.523524 1 11 UNK H51075 1.524484 2 12 CGI-90 protein AA458483 1.52448

11 8 4 AE-binding protein 1 AA490462 1.5244810 3 7 HSPC023 protein W92964 1.5254422 9 8 ESTs T72661 1.5254426 9 1 Kruppel-like factor 4 (gut) H45668 1.52648 9 3 eukaryotic translation initiation factor N92469 1.52736

29 4 13 EMPTY EMPTY 1.52736


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3 9 5 CDC-like kinase 2 AA282845 1.5283214 8 6 Homo sapiens, Similar to hypothetical pr T97890 1.5283215 6 1 tropomodulin AA235388 1.528322 9 4 Homo sapiens ryanodine receptor 2 (cardi R15791 1.52928

30 8 9 ESTs R06569 1.5292831 6 2 protein tyrosine phosphatase type IVA, m R61007 1.529287 14 5 myelin-associated oligodendrocyte basic H23197 1.53024

19 6 11 ESTs, Weakly similar to ALU1_HUMAN ALU S H64146 1.530249 12 12 serum/glucocorticoid regulated kinase R36085 1.5312

15 11 8 hypothetical protein N50686 1.532161 14 13 EMPTY EMPTY 1.53312

14 9 4 zinc finger protein 75 (D8C6) AA450115 1.5331226 14 6 Conserved gene telomeric to alpha globin AA425757 1.5331218 3 8 ESTs R23222 1.5340813 12 2 polymerase (RNA) mitochondrial (DNA dire R31115 1.5364 1 4 polymerase (DNA directed), delta 3 AA504204 1.53696

18 2 9 ESTs R89218 1.5369630 14 3 translocating chain-associating membrane H15707 1.5369631 6 8 ESTs T67138 1.5379211 13 3 potassium voltage-gated channel, shaker- AA013095 1.5388813 14 12 ESTs N54487 1.5388820 2 10 bromodomain and PHD finger containing, 3 N52994 1.5388823 13 3 regulator of G-protein signalling 16 AA453774 1.5388822 2 4 protein disulfide isomerase-related prot R01669 1.5398426 7 7 DKFZP564M112 protein W72496 1.539843 4 4 cytokine-inducible kinase AA489234 1.5408

25 2 12 RAB5A, member RAS oncogene family R45525 1.540826 14 4 RAB6A, member RAS oncogene family AA088745 1.540811 9 5 cysteine and glycine-rich protein 2 T59334 1.5436823 2 13 EMPTY EMPTY 1.5446411 1 7 Homo sapiens cDNA FLJ13495 fis, clone PL N53665 1.545618 6 13 EMPTY EMPTY 1.545618 9 13 EMPTY EMPTY 1.545619 7 1 ribose 5-phosphate isomerase A (ribose 5 N20072 1.545621 12 4 dentatorubral-pallidoluysian atrophy (at H08643 1.545631 11 13 EMPTY EMPTY 1.545611 14 4 apolipoprotein E AA478589 1.5465620 7 9 Homo sapiens clone 24538 mRNA sequence R63245 1.5465623 8 9 ESTs T97361 1.5465625 7 11 ESTs R78590 | R785 1.546568 10 11 UNK R31184 1.54752

10 10 10 ESTs AA125825 1.547521 13 6 potassium voltage-gated channel, shaker- H14383 1.54848

19 8 2 KIAA0305 gene product R26672 1.5494419 14 6 GS2 gene AA449678 1.549446 4 1 MAP/microtubule affinity-regulating kina H09721 1.5504

21 3 1 zinc finger protein 268 T57877 1.5523223 4 9 insulin-like growth factor 1 receptor AA256532 1.5523228 3 7 pyrophosphatase (inorganic) H50229 1.5532814 2 11 ESTs R98812 1.555215 7 9 ESTs T90375 1.555223 7 10 KIAA0849 protein R99326 1.5552


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7 13 9 Homo sapiens mRNA; cDNA DKFZp434J1726 (f T97183 1.5561630 5 3 small nuclear ribonucleoprotein polypept AA599116 1.5561624 5 6 ESTs, Weakly similar to putative p150 [H R96599 1.5580824 5 3 solute carrier family 6 (neurotransmitte N49856 1.5590424 9 10 Homo sapiens cDNA FLJ11041 fis, clone PL R66925 1.5590428 2 8 ESTs R23755 1.5590424 6 11 ESTs, Weakly similar to unnamed protein W93317 1.5627 10 13 EMPTY EMPTY 1.568 11 9 UNK N33589 1.56096

10 13 1 RAB9, member RAS oncogene family H98534 1.5609622 6 6 ESTs, Weakly similar to ALU7_HUMAN ALU S R67004 1.5609627 5 2 histone acetyltransferase AA057313 1.5628812 1 11 ESTs, Weakly similar to KIAA0681 protein R76499 1.564817 8 13 EMPTY EMPTY 1.564820 2 1 major histocompatibility complex, class AA458472 1.564831 14 3 phosphodiesterase 1A, calmodulin-depende AA400893 1.564818 4 4 KIAA0010 gene product AA284827 1.5657623 6 3 cadherin 11, type 2, OB-cadherin (osteob H96738 1.5657626 3 12 UNK AA454566 1.5657611 7 4 interferon-induced protein 75, 52kD T62627 1.5667219 9 6 tumor necrosis factor (ligand) superfami H54629 1.568645 4 3 Chediak-Higashi syndrome 1 N74383 1.5696

18 10 11 ESTs N54088 1.569622 1 2 UNK AA027840 1.569632 11 12 ESTs, Weakly similar to LIN1_HUMAN LINE- AA464517 1.5705611 13 6 ESTs AA465386 1.5715218 13 1 small proline-rich protein 2C AA399674 1.573448 5 7 NRAS-related gene N54372 1.5744

26 7 6 hypothetical protein MGC4276 similar to N79037 1.574412 13 5 KIAA0102 gene product AA487265 1.5753626 7 5 follicular lymphoma variant translocatio AA458992 1.5753612 7 12 eukaryotic translation initiation factor W58368 1.5782423 1 2 protein disulfide isomerase related prot N59626 1.5782431 7 12 hypothetical protein FLJ10583 T99853 1.5782432 10 4 COP9 (constitutive photomorphogenic, Ara AA461527 1.579232 1 8 hypothetical protein LOC56755 R10133 1.579211 6 13 EMPTY EMPTY 1.5801615 13 2 ATPase, H+ transporting, lysosomal (vacu AA457717 1.5801614 4 12 hypothetical protein DKFZp434N1923 AA434161 1.5811215 6 9 ESTs R36109 1.5811232 10 12 hypothetical protein AA120816 1.581125 12 5 POU domain, class 2, transcription facto H38522 1.58304

10 7 7 eukaryotic translation initiation factor N93082 1.5830424 14 7 Homo sapiens cDNA FLJ20153 fis, clone CO W95682 1.5830413 5 2 splicing factor, arginine/serine-rich (t H11720 1.5849617 3 13 EMPTY EMPTY 1.5849623 1 9 EST R33073 1.5849630 1 2 RAB11A, member RAS oncogene family AA025059 1.584967 10 2 E74-like factor 4 (ets domain transcript H14359 1.58592

11 14 13 rbcl oxygenase Cont-3 1.5859218 14 7 ribosomal protein L39 N54526 1.5859219 6 8 Friedreich ataxia region gene X123 H24395 1.58592


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27 4 1 ESTs, Weakly similar to AF161356 1 HSPC0 N68565 1.585927 5 4 lamin A/C AA496997 1.58784

20 4 3 peroxisomal membrane protein 3 (35kD, Ze AA452566 1.58882 6 5 formyl peptide receptor 1 AA425767 1.589763 6 3 ATP synthase, H+ transporting, mitochond AA453765 1.58976

19 10 2 KIAA0275 gene product AA398230 1.589763 7 12 lymphocyte-specific protein tyrosine kin AA469965 1.590727 14 11 UNK R96238 1.59072

20 9 2 PC4 and SFRS1 interacting protein 1 AA227982 1.5916811 10 2 BRCA1 associated protein-1 (ubiquitin ca H09066 1.5926424 9 3 translational inhibitor protein p14.5 N72715 1.592644 3 3 pleiotrophin (heparin binding growth fac AA001449 1.59367 3 13 EMPTY EMPTY 1.5936

22 8 3 KIAA0056 protein AA430546 1.593629 12 12 hypothetical protein FLJ10262 N49280 1.593611 7 2 sal (Drosophila)-like 2 H23254 1.5955218 4 10 Sarcolemmal-associated protein N95371 1.5955213 10 6 cytochrome c oxidase subunit VIa polypep AA482243 1.5964825 4 2 splicing factor, arginine/serine-rich 7 H54020 1.5974410 13 5 PAK-interacting exchange factor beta AA457036 1.598426 7 1 interleukin 1 receptor, type II H78386 1.598413 4 6 cystatin C (amyloid angiopathy and cereb AA599177 1.5993625 4 4 signal transducing adaptor molecule (SH3 AA488622 1.5993631 5 1 v-ets avian erythroblastosis virus E26 o H96235 1.599366 14 10 Homo sapiens cDNA FLJ20376 fis, clone HU H91044 1.60032

14 9 3 H2A histone family, member X H95392 1.6003224 10 6 ESTs R08831 1.600328 1 5 hexokinase 1 AA485272 1.60416

18 13 3 N-myristoyltransferase 1 AA436406 1.6051216 3 12 ESTs N63951 1.6060815 9 5 reticulon 3 AA430035 1.6099232 13 6 transcription elongation factor A (SII)- AA478480 1.609924 6 5 farnesyl diphosphate synthase (farnesyl T65790 1.61088

19 5 12 NCK adaptor protein 1 N90137 1.6108820 10 4 small membrane protein 1 AA402766 1.6118423 7 1 transketolase (Wernicke-Korsakoff syndro AA070358 1.6118419 2 9 ESTs, Weakly similar to ALU1_HUMAN ALU S R74480 1.612818 12 2 rearranged L-myc fusion sequence R26070 1.6137619 6 4 SKIP for skeletal muscle and kidney enri AA476625 1.6137627 3 1 small nuclear ribonucleoprotein D1 polyp H16255 1.6137627 9 5 dishevelled 2 (homologous to Drosophila AA448866 1.613765 6 2 solute carrier family 31 (copper transpo R76558 | R762 1.61664

22 10 4 xeroderma pigmentosum, complementation g AA287323 1.616647 12 4 cytochrome b-5 R91950 1.61856

28 9 5 adenosine deaminase, RNA-specific AA600189 1.6195214 12 5 myosin, light polypeptide kinase AA487215 1.6204818 7 6 elaC (E.coli) homolog 1 N52912 1.6204822 7 11 ESTs, Moderately similar to ALU7_HUMAN A N53024 1.620481 5 4 ribosomal protein L27a AA599178 1.6224

16 14 6 NADH dehydrogenase (ubiquinone) 1 alpha AA598884 1.622427 12 2 pumilio (Drosophila) homolog 2 AA496663 1.622431 9 7 hypothetical protein DKFZp761B2423 R88741 1.6224


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18 5 1 hypothetical protein PRO2706 AA485426 1.6233620 9 5 D-dopachrome tautomerase AA397824 1.623361 12 7 hypothetical protein FLJ13258 similar to AA037396 1.624322 11 3 glutathione S-transferase M1 AA290738 1.62432

29 12 3 DR1-associated protein 1 (negative cofac AA406285 1.6243214 8 11 RNA binding motif protein, X chromosome R26706 1.626247 13 2 protein phosphatase 2 (formerly 2A), cat AA490473 1.6272

11 9 8 Homo sapiens mRNA for KIAA1741 protein, R74078 1.62724 1 10 ESTs H94977 1.62816

10 6 7 CGI-45 protein H48233 1.629124 3 5 gamma-aminobutyric acid (GABA) A recepto R40790 1.63008

15 9 8 ESTs H23880 1.63219 14 12 ESTs, Moderately similar to R3HU12 ribos N74953 1.6329614 3 1 peroxisomal biogenesis factor 12 H10965 1.635841 11 5 insulin receptor AA001614 1.63776

22 3 9 Homo sapiens cDNA: FLJ23070 fis, clone L R96455 1.6377611 2 1 KIAA0128 protein; septin 2 R76459 1.639689 8 5 phosphatidylinositol 4-kinase, catalytic AA447595 1.64064

10 14 1 hydroxyacyl-Coenzyme A dehydrogenase, ty AA458661 1.6406411 2 2 holocarboxylase synthetase (biotin-[prop AA455043 1.6406422 6 7 hypothetical protein FLJ13855 R30957 1.641620 4 5 UNK W85914 1.644482 2 8 UNK R23146 1.645449 1 2 NADH dehydrogenase (ubiquinone) Fe-S pro AA055102 1.64544

18 6 7 RNA cyclase homolog R05309 1.6454427 4 2 hexabrachion (tenascin C, cytotactin) R39239 1.6454414 14 12 hypothetical protein dJ616B8.3 R96941 1.646427 11 13 EMPTY EMPTY 1.646414 4 11 cyclin T2 R63702 1.6483216 14 1 lanosterol synthase (2,3-oxidosqualene-l AA437389 1.6483218 14 1 sialyltransferase AA497051 1.6483226 4 7 discs, large (Drosophila) homolog 5 T77840 1.648325 13 2 meningioma expressed antigen 6 (coiled-c T99793 1.649286 11 6 Homo sapiens mRNA; cDNA DKFZp586I0523 (f H52535 1.64928

18 8 13 EMPTY EMPTY 1.6492828 9 6 BTB (POZ) domain containing 1 AA083385 1.6502417 7 1 ribosomal protein L19 AA083485 1.6531227 8 3 thyroid hormone receptor interactor 10 R49671 1.65623 5 3 calnexin AA126265 1.6569630 3 4 vaccinia related kinase 2 AA490617 1.657921 14 1 platelet-derived growth factor receptor, R56211 1.65888

12 12 6 CGI-150 protein N57657 1.6588826 6 3 phosphoglucomutase 1 AA488373 1.658884 2 2 Ewing sarcoma breakpoint region 1 R32756 1.6608

30 8 4 hypothetical protein FLJ12442 R42815 1.661763 12 9 ESTs R00507 1.662722 5 6 SH3-containing protein SH3GLB1 R93728 1.66368

10 9 7 hypothetical protein FLJ10441 W94253 1.6646427 11 1 protein kinase C, iota T57875 1.6646412 11 3 general transcription factor IIB H23978 1.6675224 5 2 KIAA0042 gene product AA477501 1.6675225 14 3 similar to S. cerevisiae SSM4 H67086 1.66752


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19 2 3 amiloride binding protein 1 (amine oxida T46924 1.6694422 14 6 UNK H92821 1.6723229 9 10 ESTs R25153 1.672323 5 13 EMPTY EMPTY 1.67328

17 10 1 mitogen-activated protein kinase kinase W56266 1.6742424 10 5 inositol polyphosphate-5-phosphatase, 40 T58773 1.6742417 5 6 lysyl oxidase AA452916 1.67524 7 1 hydroxy-delta-5-steroid dehydrogenase, 3 R68803 1.67808

16 5 5 phosphorylase kinase, beta AA476263 1.6780831 13 6 putative tumor suppressor R44982 1.6780818 1 2 GCN5 (general control of amino-acid synt H94857 1.6820 10 2 arginine-rich, mutated in early stage tu R91550 1.6820 14 11 ESTs H94648 1.6810 11 1 CCCTC-binding factor (zinc finger protei H89996 1.680964 2 9 ESTs R91245 1.681929 2 2 signal recognition particle 72kD AA463631 1.683849 8 4 early development regulator 2 (homolog o AA598840 1.68484 4 3 parathyroid hormone W37306 1.685764 8 10 hypothetical protein N53445 1.68864

10 12 6 ESTs, Weakly similar to Similarity to B. N54296 1.6905618 14 11 ESTs H93344 1.691525 14 1 steroid-5-alpha-reductase, alpha polypep R36874 1.69248

23 2 2 poly (ADP-ribose) glycohydrolase H95088 1.6924825 10 8 tumor endothelial marker 8 H58645 1.692488 10 6 ESTs, Moderately similar to p76 [H.sapie H65261 1.69344

19 7 7 U2(RNU2) small nuclear RNA auxillary fac W90506 1.6934428 7 2 Homo sapiens cDNA: FLJ23028 fis, clone L AA436564 1.6934432 2 6 ESTs, Weakly similar to KE03 protein [H. R11184 1.697284 10 3 glucose phosphate isomerase AA401111 1.69824

16 1 5 high-mobility group (nonhistone chromoso AA448261 1.6982416 11 9 Homo sapiens cDNA FLJ20848 fis, clone AD R76506 1.6982417 11 2 solute carrier family 9 (sodium/hydrogen R45009 1.7020823 3 6 cytochrome c-1 AA447774 1.702084 10 11 CCAAT/enhancer binding protein (C/EBP), H26183 | H26 1.704

19 11 5 UNK T47230 1.70412 9 4 ATP-binding cassette, sub-family E (OABP T70056 1.7049616 14 9 EST H51625 1.7068829 11 6 UNK AA452909 1.7068822 9 5 mannosidase, alpha, class 1A, member 2 AA455062 1.708826 4 10 ESTs N80685 1.708815 7 12 Ste20-related serine/threonine kinase AA454971 1.7097617 8 5 cytochrome c oxidase subunit Va AA491224 1.7116822 1 1 Golgi apparatus protein 1 H66617 1.7116823 6 4 jun D proto-oncogene AA418670 1.7126426 10 12 amyloid beta precursor protein (cytoplas AA431206 1.712641 13 13 EMPTY EMPTY 1.7136

15 5 3 Homo sapiens mRNA for caldesmon, 3' UTR AA076063 1.713628 3 8 Homo sapiens clone TCCCTA00142 mRNA sequ R35245 1.7193628 3 11 KIAA1155 protein H77707 1.719362 14 13 triosphosphate isomerase Cont-9 1.72224

28 5 3 CD164 antigen, sialomucin AA598561 1.7222417 14 9 ESTs R00826 1.7232


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9 9 3 thymosin, beta, identified in neuroblast N91887 1.724164 11 8 ESTs T91195 1.726082 4 5 glutathione S-transferase M4 AA486570 1.727048 2 11 Homo sapiens mRNA; cDNA DKFZp586B1722 (f R83162 1.728

29 14 6 UNK AA430744 1.72831 2 1 UDP-galactose transporter related R41839 1.72820 12 4 pyrroline-5-carboxylate synthetase (glut AA143510 1.728961 13 8 KIAA1007 protein T64885 1.72992

22 4 1 patched (Drosophila) homolog AA169807 1.7308828 3 6 mannosidase, alpha, class 1A, member 2 AA455062 1.7308832 1 11 hypothetical protein MGC5242 N53061 1.730882 7 11 mannosyl (alpha-1,6-)-glycoprotein beta- AA485653 1.731846 7 2 actin binding LIM protein 1 AA406601 1.73184

18 1 6 hypothetical protein N70864 1.7337613 14 4 polymerase (DNA directed), beta AA402855 1.7347215 4 4 leptin receptor gene-related protein H51066 1.7347217 4 13 EMPTY EMPTY 1.7366425 12 10 chromosome 4 open reading frame 1 H78368 1.736642 8 6 hypothetical protein R67915 1.7376

21 12 7 tensin H10988 1.737629 1 6 profilin 1 AA521431 1.73761 8 3 serine/threonine kinase 15 R19158 1.73856

29 14 3 putative gene product H09111 1.7385623 9 8 UNK H73058 1.7395229 12 1 v-rel avian reticuloendotheliosis viral AA443547 1.7395231 2 2 discs, large (Drosophila) homolog 4 R39954 1.7395213 14 5 nucleobindin 1 AA452535 1.7404820 8 5 KDEL (Lys-Asp-Glu-Leu) endoplasmic retic AA485911 1.7443230 4 5 RBP1-like protein AA447551 1.7462432 6 5 HIV-1 Rev binding protein AA485958 1.7462414 8 2 RAB31, member RAS oncogene family T96083 1.747222 11 1 membrane protein, palmitoylated 1 (55kD) N74236 1.7481627 13 1 serine (or cysteine) proteinase inhibito N54794 1.7481624 7 4 TGFB1-induced anti-apoptotic factor 1 AA446223 1.7500817 14 10 hypothetical protein FLJ20392 H69785 1.7510431 10 11 ESTs N73843 1.752966 4 6 uncharacterized hypothalamus protein HCD R65792 1.754887 2 7 ESTs, Weakly similar to ALU7_HUMAN ALU S R68464 1.75488

18 13 9 ESTs R33336 1.7548832 4 2 leptin receptor gene-related protein H51066 1.7558432 4 9 ESTs H38230 1.7558426 7 9 uncharacterized hypothalamus protein HT0 H81010 1.756810 9 1 vascular endothelial growth factor C H07899 1.7577622 5 8 ESTs H63357 1.758727 5 13 EMPTY EMPTY 1.75968

14 1 2 Pirin H69335 1.7596814 12 4 ubiquitin-activating enzyme E1 (A1S9T an AA598670 1.7606428 9 7 CGI-26 protein N74602 1.760646 3 3 UNK AA486085 1.76448

25 7 6 apyrase, lysosomal AA452541 1.7644828 4 10 protocadherin 20 AA040057 1.7644810 13 3 glycine cleavage system protein H (amino R28294 1.76544


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28 14 3 fumarate hydratase AA026918 1.7683229 2 12 ESTs T71629 1.7683218 6 1 inhibitor of DNA binding 1, dominant neg AA457158 1.771218 7 4 high density lipoprotein binding protein AA457697 1.771222 6 12 ESTs, Weakly similar to organic anion tr AA033972 1.771223 13 10 ESTs R31580 1.771210 1 11 Homo sapiens clone CDABP0086 mRNA sequen H91332 1.7731215 7 6 UNK AA442092 1.7731218 5 8 ESTs N55259 1.774088 8 2 speckle-type POZ protein R35231 1.77504

31 5 4 latent transforming growth factor beta b AA490011 1.7769629 14 12 ESTs, Moderately similar to ALU8_HUMAN A N54416 1.7779219 6 5 thyroid hormone receptor interactor 4 AA476490 1.7788815 2 11 ESTs N62554 1.7798420 8 9 Homo sapiens complement-c1q tumor necros R16812 1.780828 13 6 putative nucleic acid binding protein RY AA293192 1.78085 13 5 PCTAIRE protein kinase 3 AA394136 1.78176

11 7 1 LIM domain only 2 (rhombotin-like 1) AA464544 1.7836829 11 4 solute carrier family 1 (glial high affi AA453742 1.7846432 8 4 KIAA0210 gene product AA278840 1.7846416 4 2 mature T-cell proliferation 1 AA029308 1.788484 11 10 ESTs H47358 1.7904

26 8 6 LIS1-interacting protein NUDEL; endoolig R94693 1.79041 13 2 KIAA0244 protein W95864 1.79136

13 11 5 splicing factor, arginine/serine-rich 4 AA496787 1.791366 12 10 ESTs R69589 1.79232

20 11 6 selenoprotein T R78516 1.792324 2 4 hect domain and RLD 3 AA282253 1.79328

26 1 12 presenilins associated rhomboid-like pro AA131464 1.7961627 14 5 syntaxin 3A AA436871 1.7971211 4 4 Src-like-adapter AA485141 1.799046 11 4 vascular cell adhesion molecule 1 H16591 1.8

10 14 7 ribosomal protein L26 homolog W94107 1.828 13 4 proteolipid protein (Pelizaeus-Merzbache R45264 1.828 6 6 hypothetical protein FLJ21016 R11630 1.822 2 6 DEAD/H (Asp-Glu-Ala-Asp/His) box polypep R08935 1.8009618 7 1 interleukin 1 receptor, type I AA464526 1.8019226 5 4 KIAA0005 gene product AA428029 1.8028824 8 8 EST T77883 1.804827 6 1 eukaryotic translation initiation factor H54752 1.80486 12 13 EMPTY EMPTY 1.80864

23 9 13 EMPTY EMPTY 1.8086426 12 4 RD RNA-binding protein AA056390 1.8086432 2 11 ribosomal protein L37a N57731 1.8086429 14 2 proteasome (prosome, macropain) 26S subu AA497055 1.809630 9 5 Homo sapiens mRNA; cDNA DKFZp762O2215 (f AA102257 1.809610 1 12 chromosome 14 open reading frame 1 N70492 1.811521 4 5 cullin 2 AA454094 1.812483 10 5 RNA-binding protein (autoantigenic) AA504617 1.8144

27 7 3 KIAA0286 protein T81400 1.81449 10 4 CDC28 protein kinase 1 AA459292 1.81632

26 8 9 KIAA1008 protein H04004 1.81632


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25 7 8 hypothetical protein FLJ14153 T96603 1.817288 9 7 Sec61 gamma W96107 1.81824

10 13 2 U2 small nuclear ribonucleoprotein auxil T67521 1.8182417 4 5 C-terminal binding protein 1 AA478268 1.8182428 14 10 hypothetical protein PRO0823 R88734 1.8182422 11 7 oncogene TC21 R21416 1.820164 9 2 microfibrillar-associated protein 1 R01211 1.82304

29 11 9 ESTs T95274 1.823042 10 2 LIV-1 protein, estrogen regulated H29315 1.824

30 10 5 translocating chain-associating membrane AA452556 1.82431 11 6 CD81 antigen (target of antiproliferativ AA486556 1.8249627 10 4 CD34 antigen AA434387 1.8259223 4 5 syntrophin, beta 2 (dystrophin-associate AA491439 1.827848 9 9 ESTs R07322 1.82976

22 7 6 frizzled (Drosophila) homolog 4 R26141 1.8297616 12 9 UNK H93463 1.8307227 7 6 EST R22306 1.830722 13 3 microsomal glutathione S-transferase 1 AA495936 1.832645 8 1 tyrosine 3-monooxygenase/tryptophan 5-mo N69107 1.832646 7 5 dual-specificity tyrosine-(Y)-phosphoryl AA450003 1.83264

24 14 11 ESTs H68477 1.8326426 2 10 zinc-fingers and homeoboxes 1 H94323 1.8345632 10 11 ubiquitin-conjugating enzyme E2N (homolo AA490124 1.8364829 6 4 lactate dehydrogenase A AA497029 1.837441 4 4 SELENOPHOSPHATE SYNTHETASE ; Human seAA488081 1.83844 3 6 ESTs, Weakly similar to PMX1_HUMAN PAIRE R69071 1.8384

29 12 5 splicing factor, arginine/serine-rich 1 T65786 1.8393630 14 1 procollagen-lysine, 2-oxoglutarate 5-dio AA476241 1.8403211 10 5 brain and reproductive organ-expressed ( AA479741 1.8412812 10 5 diacylglycerol kinase, alpha (80kD) AA456830 1.8412828 2 4 adenylate cyclase 7 AA125779 1.8412832 7 1 NADH dehydrogenase (ubiquinone) Fe-S pro AA406536 1.841287 5 12 polymerase (RNA) II (DNA directed) polyp AA458646 1.84512

30 4 2 proteasome (prosome, macropain) 26S subu AA454852 1.846085 10 5 phosphoinositide-3-kinase, regulatory su R54050 1.85088

30 7 5 Ras-related GTP-binding protein AA406613 1.850882 6 6 hypothetical protein FLJ13340 R92952 1.85184

13 6 5 poly(rC)-binding protein 1 AA490047 1.851842 2 6 CGI-134 protein N64429 1.8528

11 4 3 activated leucocyte cell adhesion molecu R39862 1.852812 14 2 O-linked N-acetylglucosamine (GlcNAc) tr R40794 1.852820 11 2 TATA box binding protein (TBP)-associate AA045588 1.8547227 12 5 ubiquitin fusion degradation 1-like T57841 1.8556828 7 11 ESTs H19668 1.8566431 12 7 maternally expressed 3 R98695 1.857611 12 12 actin related protein 2/3 complex, subun H73961 1.8585618 9 4 methionine adenosyltransferase II, alpha T59245 1.8595225 11 3 hyaluronoglucosaminidase 1 AA464196 1.860483 11 10 ESTs R53917 1.861449 12 10 KIAA0553 protein R93149 1.86144

11 5 5 zinc finger protein 162 AA454673 1.861447 14 13 RUBISCO activase Cont-2 1.8624


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12 2 4 KIAA0033 protein AA450037 1.862432 3 3 superoxide dismutase 1, soluble (amyotro AA599127 1.867214 13 3 aminopeptidase puromycin sensitive R32450 1.8681615 6 2 protein phosphatase 2 (formerly 2A), reg R55800 1.8681625 14 6 heme-regulated initiation factor 2-alpha AA486430 1.8681614 4 3 spectrin, alpha, non-erythrocytic 1 (alp T60117 1.869121 12 5 UNK T47815 1.87008

28 14 13 Yamagata-TBD UNK 1.870084 8 4 forkhead box F1 AA112660 1.87296

28 6 5 heterochromatin-like protein 1 AA132226 1.8748822 8 7 cartilage associated protein N75346 1.8777626 10 1 potassium channel, subfamily K, member 1 N62620 1.877762 8 2 GS3955 protein AA458653 1.88064

27 6 12 jun B proto-oncogene N94468 1.880648 14 3 dolichyl-phosphate (UDP-N-acetylglucosam R55620 1.88161 11 7 vesicle transport-related protein AA027992 1.88544

10 10 9 peroxisome proliferative activated recep W96146 1.8892817 13 6 proteasome (prosome, macropain) activato AA486324 1.8892824 3 5 protein phosphatase 1, regulatory subuni AA459351 1.8892827 3 10 GCIP-interacting protein p29 H56931 1.8892817 14 5 eukaryotic translation initiation factor AA598863 1.8921619 4 4 hypothetical protein FLJ20693 AA393856 1.8931227 4 5 B-cell associated protein AA464567 1.8931219 9 5 nuclease sensitive element binding prote AA599175 1.89623 1 5 prostate tumor over expressed gene 1 AA488053 1.8969615 6 4 mitogen-activated protein kinase kinase AA293365 1.897926 5 3 single-stranded DNA-binding protein R05694 1.89984

15 8 4 integrin, alpha E (antigen CD103, human AA425451 1.8998419 7 5 ribonuclease 6 precursor AA405000 1.899847 9 7 zinedin H90310 1.90083 3 9 UNK R63530 1.901761 13 1 prion protein (p27-30) (Creutzfeld-Jakob AA455969 1.90368

22 12 5 death-associated protein 6 AA487253 1.9046427 13 8 hypothetical protein FLJ11342 N63866 1.904643 13 8 ESTs, Moderately similar to CAD8_HUMAN C R56219 1.90564 4 6 fatty-acid-Coenzyme A ligase, long-chain N98509 1.9056

11 4 2 heterogeneous nuclear ribonucleoprotein W02101 1.9065612 14 9 hypothetical protein FLJ13164 R22420 1.906567 14 12 ESTs N55446 1.90848

10 2 1 ralA binding protein 1 AA085619 1.9094430 2 13 EMPTY EMPTY 1.9094423 5 4 lamin B2 AA457025 1.910426 4 1 jun B proto-oncogene T99236 1.910423 12 6 CD53 antigen AA132090 1.912326 5 5 spermine synthase AA136125 1.91904

14 1 4 reticulocalbin 1, EF-hand calcium bindin AA457719 1.9219225 14 4 bone morphogenetic protein 6 AA424833 1.9219219 4 6 CBF1 interacting corepressor R53998 1.9228813 12 11 Homo sapiens mRNA; cDNA DKFZp762G207 (fr R93089 1.924826 12 1 3-oxoacid CoA transferase R40897 1.92486 12 7 phosphatidylcholine transfer protein AA029757 1.92576

19 3 2 high-mobility group (nonhistone chromoso AA019203 1.92864


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10 1 3 protein phosphatase 2 (formerly 2A), cat AA599092 1.929615 10 1 ras homolog gene family, member B AA495790 1.92965 5 4 cell division cycle 25B AA448659 1.9344

11 13 1 glutathione peroxidase 4 (phospholipid h AA454856 1.934425 2 5 tissue specific transplantation antigen AA421688 1.94416 13 12 CREBBP/EP300 inhibitory protein 1 N54914 1.9459211 6 3 ATPase, Ca++ transporting, cardiac muscl H85355 1.948811 5 6 eukaryotic translation elongation factor AA620477 1.948814 1 12 protein S (alpha) T74192 1.950724 4 7 laminin, beta 1 W74293 1.951686 14 6 heterogeneous nuclear ribonucleoprotein AA490991 1.95369 3 3 annexin A1 H63077 1.9536

28 1 12 adaptor-related protein complex 4, sigma N32766 1.9545629 7 13 EMPTY EMPTY 1.9545611 13 10 ESTs R94659 1.9564820 7 11 guanine nucleotide binding protein (G pr H79130 1.956487 12 6 membrane-spanning 4-domains, subfamily A N91385 1.95744

20 11 4 RecQ protein-like (DNA helicase Q1-like) AA456585 1.9574427 4 6 chaperonin containing TCP1, subunit 4 (d AA598637 1.960326 6 4 UNK H14208 1.96128

26 1 5 UNK AA448487 1.9641615 7 3 ring finger protein 5 AA402960 1.9651217 10 4 Homo sapiens mRNA; cDNA DKFZp586C1924 (f T87078 1.9651230 9 12 uncharacterized hematopoietic stem/proge AA431199 1.9660817 10 3 insulin induced gene 1 H59620 1.9708823 2 5 nucleotide binding protein AA482229 1.970881 8 6 huntingtin interacting protein 2 H78385 1.97184

19 11 3 eukaryotic translation initiation factor R86305 1.9718428 6 1 mitochondrial translational initiation f H18070 1.9718410 6 5 G-rich RNA sequence binding factor 1 AA485646 1.974727 8 6 capping protein (actin filament), gelsol AA486766 1.976647 4 11 ESTs N65972 1.976642 7 4 chromobox homolog 1 (Drosophila HP1 beta AA448667 1.97856

16 10 4 v-abl Abelson murine leukemia viral onco AA496785 1.9804823 10 8 hypothetical protein FLJ10154 T91807 1.980483 14 8 KIAA0630 protein R28649 1.983363 7 7 UNK R86944 1.98432

18 10 12 CGI-142 AA455020 1.9852815 6 6 collagen, type I, alpha 2 AA490172 1.9862427 3 5 prefoldin 4 AA253430 1.9862419 13 5 triadin AA404293 1.987217 12 2 topoisomerase (DNA) II binding protein R97785 1.989121 10 1 PTK7 protein tyrosine kinase 7 AA453789 1.990086 5 6 UNK H93118 1.99008

30 5 5 monocyte to macrophage differentiation-a AA487434 1.9900826 5 3 phosphoribosyl pyrophosphate synthetase AA151486 1.9910426 6 4 pleckstrin homology, Sec7 and coiled/coi AA480859 1.9948821 13 10 hypothetical protein FLJ12752 R28287 1.995846 8 7 tubulin-specific chaperone a N93021 1.99776

14 12 12 UNK N57002 1.998721 14 9 hypothetical protein FLJ10922 H82874 1.99968

29 4 12 KIAA1610 protein R78536 | R784 2.00064


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18 5 4 nuclear receptor subfamily 1, group D, m AA425685 2.0035219 12 7 down-regulator of transcription 1, TBP-b AA132007 2.003524 5 4 sortilin-related receptor, L(DLR class) T51538 2.00448

32 3 10 KIAA1228 protein H72675 2.0054411 1 12 RAB6A, member RAS oncogene family H20138 2.007365 6 5 calponin 2 AA284856 2.00832

23 1 4 multiple endocrine neoplasia I AA243439 2.0083229 11 2 myeloid leukemia factor 2 AA457726 2.009288 3 2 UNK AA100296 2.010249 11 5 insulin-like growth factor 2 (somatomedi N54596 2.01024

10 8 3 protein phosphatase 1, regulatory (inhib AA485347 2.011225 12 3 spectrin SH3 domain binding protein 1 R16667 2.01125 10 8 ESTs R16074 2.01216

23 7 5 intersectin 1 (SH3 domain protein) AA496795 2.013121 7 5 tumor rejection antigen (gp96) 1 AA598758 2.01696

14 4 7 glycine cleavage system protein H (amino R71328 2.0169611 11 5 ubiquitin-like 1 (sentrin) AA488626 2.0179214 2 12 O-linked N-acetylglucosamine (GlcNAc) tr AA425655 2.0198423 7 3 chromosome 6 open reading frame 9 AA410896 2.0217631 13 5 NCK adaptor protein 1 AA280214 2.0227229 6 13 EMPTY EMPTY 2.023682 6 2 Human clone 23589 mRNA sequence AA253479 2.02562 2 5 5T4 oncofetal trophoblast glycoprotein AA425666 2.0256

23 14 7 peroxiredoxin 5 N68467 2.0284825 5 8 hypothetical protein FLJ10707 N68660 2.028481 14 11 ESTs H55909 2.029446 14 12 frizzled-related protein H87275 2.03136

32 9 6 putative zinc finger protein H26155 | H26 2.0313623 10 6 cyclin-dependent kinase 8 R59697 2.0323232 10 8 ESTs R93374 2.0332812 6 3 proteasome (prosome, macropain) subunit, AA465237 2.0342430 10 7 calumenin R78586 | R785 2.034245 9 12 ESTs, Weakly similar to T08675 hypotheti H66612 2.037121 9 5 integrin cytoplasmic domain-associated p AA457038 2.03808

25 10 2 KIAA0321 protein N74380 2.0380818 10 2 bullous pemphigoid antigen 1 (230/240kD) H44785 2.0412 11 8 Homo sapiens cDNA FLJ20653 fis, clone KA N67039 2.042884 14 1 suppression of tumorigenicity 13 (colon H65676 2.0448

15 3 2 phytanoyl-CoA hydroxylase (Refsum diseas N63845 2.044818 3 11 ESTs W15465 2.044832 1 5 histidyl-tRNA synthetase H61209 2.044827 9 3 ATP binding protein associated with cell N80741 2.0467225 6 1 ribosomal protein S5 AA456616 2.047684 7 7 voltage-dependent anion channel 3 H38623 2.05056

21 12 3 dedicator of cyto-kinesis 1 W02657 2.0524822 12 12 CGI-107 protein AA004210 2.0534411 2 4 ATPase, Na+/K+ transporting, beta 3 poly AA486417 2.054418 9 11 ESTs R23212 2.0553625 13 3 DEAD/H (Asp-Glu-Ala-Asp/His) box polypep AA032090 2.0553611 11 3 chromosome 18 open reading frame 1 AA489633 2.0563214 4 5 suppression of tumorigenicity 5 AA446017 2.0563212 12 3 gamma-aminobutyric acid (GABA) A recepto R45230 2.05824


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24 8 7 Homo sapiens mRNA; cDNA DKFZp434D2111 (f R67562 2.0620818 8 5 DEAD/H (Asp-Glu-Ala-Asp/His) box polypep AA465387 2.0678432 6 11 ESTs N92380 2.068826 12 5 KIAA0174 gene product T62546 2.0726431 8 3 cadherin 13, H-cadherin (heart) R41787 2.0745624 12 4 transcription factor 12 (HTF4, helix-loo AA488366 2.0774418 10 4 mitogen-activated protein kinase 1 W45690 2.078423 14 13 cystein endopeptidase Cont-6 2.078414 13 5 MAD2 (mitotic arrest deficient, yeast, h AA481076 2.0812832 11 5 UNK AA019332 2.0812814 13 2 corticotropin releasing hormone R45054 2.083231 1 2 UNK N92646 2.083220 5 5 fibronectin 1 R62612 2.0841630 2 1 ATX1 (antioxidant protein 1, yeast) homo AA418694 2.0841631 10 5 hypothetical protein 24636 N62562 2.084165 12 2 ATP synthase, H+ transporting, mitochond H47080 2.08512

12 14 4 protein tyrosine phosphatase, non-recept AA446259 2.0851215 3 6 cytochrome c R52654 2.0860831 14 8 oral-facial-digital syndrome 1 gene N57749 2.0899223 12 4 defender against cell death 1 AA455281 2.0985626 10 6 putative membrane protein T98298 2.0985629 8 5 GTP-binding protein homologous to Saccha T60070 2.098569 9 12 caspase 3, apoptosis-related cysteine pr AA011446 2.09952

25 5 3 adenylosuccinate lyase AA455931 2.099529 5 4 ribosomal protein L28 AA486746 2.10048

28 13 7 3-hydroxyisobutyrate dehydrogenase N55113 2.1043214 10 7 RAP1B, member of RAS oncogene family W53016 2.1052816 5 1 phosphatidylserine synthase 1 H28984 2.1052823 3 5 capping protein (actin filament) muscle AA449753 2.1100832 14 2 PTH-responsive osteosarcoma B1 protein T58251 2.1100830 5 4 fucosyltransferase 8 (alpha (1,6) fucosy AA192422 2.1139231 11 5 ornithine decarboxylase 1 AA461467 2.1158428 2 11 polymerase (RNA) II (DNA directed) polyp H73947 2.116825 2 9 UDP-glucose dehydrogenase R62173 2.1177623 9 7 pregnancy specific beta-1-glycoprotein 9 R69567 2.118728 13 7 NADH dehydrogenase (ubiquinone) 1 beta s AA032077 2.12448

18 3 4 KIAA0029 protein AA504206 2.126412 7 4 tetraspan 3 AA284492 2.1273629 6 9 TATA box binding protein (TBP)-associate R34694 2.1292811 8 3 CD151 antigen AA443118 2.1321626 9 4 S-adenosylhomocysteine hydrolase AA485626 2.132164 12 3 guanine nucleotide binding protein (G pr R96220 2.13504

14 14 6 glycerol-3-phosphate dehydrogenase 1 (so AA193116 2.1350428 7 4 golgi autoantigen, golgin subfamily a, 4 AA460981 2.1369611 3 5 actin related protein 2/3 complex, subun H25917 2.138886 7 11 aldo-keto reductase family 1, member C1 H70554 2.139847 14 3 polymerase (DNA-directed), delta 4 AA457710 2.13984

32 12 10 ESTs R70948 2.1398430 8 5 glutaminyl-peptide cyclotransferase (glu AA282134 2.1417619 8 1 renin AA458630 2.1475220 9 7 leucine aminopeptidase R69307 2.148483 10 4 chitinase 3-like 1 (cartilage glycoprote AA434115 2.15232


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32 14 3 chloride channel, nucleotide-sensitive, T52362 2.154243 7 1 retinoic acid receptor, beta AA419238 2.15808

11 7 3 HYA22 protein R01638 2.161924 14 3 Friedreich ataxia AA253413 2.167686 11 2 guanine monphosphate synthetase N59764 2.16768

31 7 8 ESTs T67448 2.1676829 3 5 Human clone 23721 mRNA sequence AA455225 2.1715223 5 8 DKFZP727A071 protein T67094 2.173446 7 4 KIAA0251 protein T69432 2.17536

28 12 7 chromosome 14 open reading frame 2 T90621 2.1772827 5 12 RAB1, member RAS oncogene family N69689 2.1811223 10 4 dihydropyrimidine dehydrogenase AA430625 2.1830427 3 6 propionyl Coenzyme A carboxylase, alpha AA608575 2.1830432 7 7 Homo sapiens mRNA; cDNA DKFZp434D0818 (f N95578 2.18428 3 5 glial fibrillary acidic protein AA069414 2.1849623 12 8 protein phosphatase 3 (formerly 2B), reg AA018907 2.185927 13 6 interferon gamma receptor 2 (interferon AA449440 2.1888

29 1 9 Homo sapiens mRNA; cDNA DKFZp434E033 (fr R31708 2.190727 4 2 stearoyl-CoA desaturase (delta-9-desatur R00707 2.19264

23 4 2 neuropilin 2 R37519 2.1926429 9 12 ESTs, Weakly similar to CA13_HUMAN COLLA R32840 2.1926430 6 2 KIAA0094 protein H73080 2.1926428 2 5 glutathione S-transferase A2 T73468 2.193625 5 5 polymyositis/scleroderma autoantigen 1 ( AA458994 2.198425 8 9 DKFZP564B0769 protein W94539 2.200325 7 3 v-myc avian myelocytomatosis viral oncog R62813 2.2032

26 11 5 ubiquitin specific protease 10 AA455233 2.204165 12 6 sperm specific antigen 2 AA496804 2.20512

16 5 2 centaurin beta2 AA490493 2.2070419 7 3 immunoglobulin superfamily containing le H62387 2.2099224 5 4 bromodomain adjacent to zinc finger doma W88615 2.2099214 1 1 caspase 3, apoptosis-related cysteine pr R42530 2.2118416 7 5 guanidinoacetate N-methyltransferase AA521337 2.2166410 11 5 C9orf10 protein AA434099 2.217628 6 3 DNA segment on chromosome X and Y (uniqu AA496902 2.217621 11 4 eukaryotic translation termination facto AA456664 2.224323 1 1 UNK T48741 2.22528

22 8 1 TIA1 cytotoxic granule-associated RNA-bi AA427664 2.2310423 4 6 UNK T68274 2.23214 10 5 polymerase (RNA) II (DNA directed) polyp AA458646 2.232962 4 4 KIAA0008 gene product AA262212 2.23488

27 6 5 thyroid hormone receptor interactor 7 AA431321 2.236818 8 6 presenilins associated rhomboid-like pro T67440 2.2387223 9 3 gamma-aminobutyric acid (GABA) A recepto H63532 2.2396824 4 5 ubiquitin specific protease 9, X chromos AA425628 2.2396829 3 2 protein tyrosine phosphatase, receptor t R45941 2.241630 13 4 ubiquinol-cytochrome c reductase, Rieske AA448184 2.2435214 14 3 actin related protein 2/3 complex, subun AA490209 2.2444829 12 4 deoxycytidine kinase H05489 2.244482 7 8 Homo sapiens mRNA; cDNA DKFZp434K1421 (f N55362 2.24544

29 5 5 mannosyl (alpha-1,6-)-glycoprotein beta- AA485653 2.2454415 4 8 Golgi protein R62968 2.24928


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28 1 6 vitamin A responsive; cytoskeleton relat R78725 2.251232 14 10 PRO1912 protein R78598 | R785 2.251222 10 2 potassium inwardly-rectifying channel, s T94781 2.252166 12 3 nucleoporin 153kD R40059 2.25408

22 9 6 zinc finger protein 22 (KOX 15) R37224 2.2540831 11 7 interleukin 1 receptor accessory protein R35903 2.254084 12 5 KIAA0112 protein; homolog of yeast ribos AA100613 2.256

22 13 5 zinc finger protein 220 AA599173 2.2569623 8 6 carbonyl reductase 1 AA280846 2.2579211 2 3 UNK AA490256 2.258881 14 3 transcription elongation factor B (SIII) W81685 2.266561 11 8 hypothetical protein DKFZp762E1312 T66935 2.266568 2 1 Homo sapiens cDNA: FLJ21927 fis, clone H N53771 2.2704

10 13 6 adenovirus 5 E1A binding protein AA598473 2.2723228 11 9 ESTs AA024488 2.2732814 6 3 signal recognition particle 54kD AA599078 2.278089 8 8 Homo sapiens clone 24921 mRNA sequence T97925 2.2848

12 11 2 TATA box binding protein (TBP)-associate AA063580 2.2876812 11 5 ubiquitination factor E4A (homologous to AA447528 2.289628 8 11 achaete-scute complex (Drosophila) homol AA441935 2.28969 10 2 KIAA0310 gene product AA454812 2.301127 9 5 suppression of tumorigenicity 16 (melano AA281635 2.30592

22 1 11 hypothetical protein DC42 N77877 2.3068817 2 2 arginyl aminopeptidase (aminopeptidase B R64101 2.3078422 4 5 phosphatidic acid phosphatase type 2A AA598865 2.30887 9 8 Homo sapiens cDNA: FLJ22003 fis, clone H N63936 2.31168

20 5 2 AD-017 protein H94897 2.3116819 6 12 RAP1B, member of RAS oncogene family W53016 2.3126419 5 5 zinc finger protein 193 AA252169 2.313622 12 10 thyroid hormone receptor-associated prot R68631 2.3145622 5 7 C-terminal binding protein 2 W49715 2.3155210 3 8 KIAA1223 protein R23227 2.3174429 7 12 KIAA0831 protein R71623 2.3174425 4 1 Sjogren syndrome antigen B (autoantigen H29485 2.3193622 11 8 ESTs T90797 2.3222426 1 1 KIAA1504 protein N73827 2.3222425 8 5 paraoxonase 2 AA446028 2.32321 9 6 ESTs AA075299 2.325121 9 1 RAD52 (S. cerevisiae) homolog N53449 2.32704

15 14 2 hepatoma-derived growth factor (high-mob AA453749 2.3270418 5 6 HSPC038 protein AA055193 2.3289627 2 4 splicing factor, arginine/serine-rich 9 AA491213 2.332817 6 1 ribosomal protein L7a H23422 2.3356821 14 3 mitogen-activated protein kinase 7 H39192 2.3356821 6 5 thioredoxin peroxidase (antioxidant enzy AA459663 2.33769 8 6 conserved gene amplified in osteosarcoma AA404264 2.33856

13 13 1 acetyl-Coenzyme A acetyltransferase 2 (a R46821 2.3385616 13 1 laminin, alpha 4 R43734 2.342420 6 3 proteasome (prosome, macropain) subunit, AA598815 2.3433625 7 5 HRIHFB2206 protein AA521422 2.3452832 6 4 KIAA0232 gene product AA406589 2.347213 10 3 far upstream element (FUSE) binding prot N81076 2.35104


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20 7 4 glutathione-S-transferase like; glutathi AA441895 2.3529617 4 12 SET translocation (myeloid leukemia-asso N52911 2.3587210 13 9 Williams-Beuren syndrome chromosome regi R70541 2.3625611 5 4 ubiquitin-conjugating enzyme E2 variant AA448676 2.3635217 12 1 proteasome (prosome, macropain) subunit, AA070997 2.364483 3 1 serum amyloid A1 H25546 2.3664

25 13 8 ubiquinol-cytochrome c reductase (6.4kD) R46837 2.3673631 8 8 ESTs R16101 2.367366 1 5 actin related protein 2/3 complex, subun AA188179 2.36832

26 10 5 electron-transfer-flavoprotein, alpha po T57919 2.3683219 10 6 programmed cell death 5 AA156940 2.3702429 12 7 ATPase, H+ transporting, lysosomal (vacu R73402 | AI66 2.3740832 10 10 KIAA1571 protein R68091 2.3788830 2 5 RNA binding motif protein, X chromosome AA487442 2.3798422 11 2 Human hbc647 mRNA sequence R31110 2.3827222 6 5 thyroid hormone receptor coactivating pr AA481555 2.385631 9 12 NS1-associated protein 1 R21425 2.387525 11 2 small inducible cytokine subfamily A (Cy T64134 2.38848

28 10 12 dynamin 1-like AA431972 2.390428 1 5 monokine induced by gamma interferon AA131406 2.3913620 2 6 leucine zipper protein 1 H73731 2.3961621 13 9 ESTs N71442 2.396161 11 12 EST H25761 | AI66 2.39712

15 7 2 kallikrein 6 (neurosin, zyme) AA454743 2.4019214 10 3 progesterone binding protein N66942 2.4028819 1 5 Ras-GTPase-activating protein SH3-domain AA449834 2.4067210 14 4 NAD(P)H menadione oxidoreductase 2, diox AA490938 2.4115228 4 9 lectin, galactoside-binding, soluble, 8 R92197 2.4115224 6 3 activin A receptor, type I AA136882 2.4153624 10 3 synaptobrevin-like 1 R27550 2.4172814 2 2 methyltransferase-like 1 AA422058 2.418248 12 4 topoisomerase (DNA) II alpha (170kD) AA504348 2.42016

15 3 10 methyl-CpG binding domain protein 4 AA010492 2.4211219 10 5 estrogen receptor binding site associate T50699 2.4249624 2 5 nucleosome assembly protein 1-like 1 R93829 2.426884 12 4 prothymosin, alpha (gene sequence 28) R98842 2.4288

11 9 3 hypothetical protein, expressed in osteo AA410188 2.428814 12 1 major histocompatibility complex, class AA158396 2.428810 8 7 BCL2/adenovirus E1B 19kD-interacting pro AA025112 2.4316823 3 3 clusterin (complement lysis inhibitor, S AA130017 2.4364826 7 4 histidyl-tRNA synthetase-like T70037 2.4364820 14 9 ESTs R25398 2.4374432 8 7 serum/glucocorticoid regulated kinase H74007 2.4374421 13 3 general transcription factor II, i, pseu AA115186 2.443229 1 4 neural precursor cell expressed, develop AA442095 2.4470419 3 5 BCL2-related protein A1 AA459263 2.4487 11 7 hypothetical protein FLJ11618 AA026662 2.4528

22 14 5 SWI/SNF related, matrix associated, acti AA481026 2.45761 2 12 TRK-fused gene R60847 2.45856

10 4 4 KIAA0009 gene product AA459109 2.4585631 9 6 calnexin AA126265 2.459524 4 1 UNK H13300 2.46048


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22 12 4 ubiquitin-conjugating enzyme E2A (RAD6 h AA600173 2.462423 14 6 hypothetical protein FLJ23018 AA281347 2.4633616 10 3 chondroitin sulfate proteoglycan 4 (mela R53541 2.4652828 4 3 solute carrier family 25 (mitochondrial AA486200 2.4691229 1 1 platelet-activating factor acetylhydrola AA464238 2.4700818 2 7 F-box only protein 21 N52450 2.4710425 5 4 UNK AA196465 2.472961 14 5 H3 histone, family 3B (H3.3B) AA608514 2.48064

24 5 5 papillary renal cell carcinoma (transloc AA488180 2.4835222 10 6 Homo sapiens mRNA; cDNA DKFZp761J139 (fr W74748 2.494082 2 3 proteasome (prosome, macropain) subunit, R71913 2.495045 10 6 cystatin B (stefin B) H22919 2.498885 13 1 CD5 antigen (p56-62) AA406028 2.49984

18 12 5 KIAA0164 gene product H21107 2.499841 14 8 CCR4-NOT transcription complex, subunit R77125 | R77 2.5008

26 2 2 flavin containing monooxygenase 5 H52119 2.50088 10 5 exportin 1 (CRM1, yeast, homolog) T59055 2.50176

30 8 3 ribosomal protein L10a R01139 2.505627 10 3 catenin (cadherin-associated protein), d H04986 2.506566 3 1 peripheral myelin protein 22 R26732 2.50752

10 13 4 IQ motif containing GTPase activating pr AA598496 2.5075211 9 6 UNK R79935 2.5094425 7 3 squalene epoxidase R01118 2.5113618 6 5 cyclin C AA451686 2.5142417 7 12 RAB2, member RAS oncogene family W60890 2.5190428 3 3 polymerase (RNA) II (DNA directed) polyp AA479052 2.522884 7 3 forkhead box F1 AA112660 2.52488 10 3 laminin, alpha 5 AA459289 2.528648 14 13 Yamagata-TBD UNK 2.5296

10 11 4 UNK AA598582 2.531526 11 7 topoisomerase (DNA) II alpha (170kD) AA504348 2.53344

16 9 2 heterogeneous nuclear ribonucleoprotein W96058 2.5353610 8 2 capping protein (actin filament) muscle AA083228 2.537286 5 2 KIAA0107 gene product AA424807 2.53921 6 3 acyl-Coenzyme A dehydrogenase, C-4 to C- N70794 2.54208

18 12 4 RYK receptor-like tyrosine kinase T77811 2.5420832 2 12 Homo sapiens mRNA; cDNA DKFZp761I141 (fr AA126901 2.5420831 9 2 SKI-INTERACTING PROTEIN H17512 2.548822 3 1 ATP-binding cassette, sub-family D (ALD) H25923 2.5516822 14 3 TG-interacting factor (TALE family homeo R83270 2.55369 14 5 NGFI-A binding protein 1 (ERG1 binding p AA488645 2.55456

18 10 5 early growth response 1 AA486533 2.5555228 3 12 nucleolar protein family A, member 3 (H/ AA464531 2.5555231 8 10 UNK H53051 2.5622423 5 5 Probe hTg737 (polycystic kidney disease, AA481279 2.5651210 14 2 SWI/SNF related, matrix associated, acti H24688 2.5756811 2 7 protein phosphatase 1, regulatory (inhib N52605 2.5785626 9 3 mitogen-activated protein kinase 6 H17504 2.5795212 14 12 Homo sapiens mRNA; cDNA DKFZp564O2363 (f AA410260 2.581446 8 5 immunoglobulin (CD79A) binding protein 1 AA463498 2.58432

18 1 5 DEAD/H (Asp-Glu-Ala-Asp/His) box polypep AA425687 2.5852823 10 7 ninein (GSK3B interacting protein) R14959 2.5872


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22 2 9 ESTs, Weakly similar to JH0148 nucleolin R54855 2.5891223 5 6 complement component 1, q subcomponent, T71284 2.591048 8 11 hypothetical protein FLJ21174 N73975 2.59488

31 9 9 KIAA0697 protein H55953 2.596823 7 12 cardiac ankyrin repeat protein AA488072 2.6006418 2 1 SRB7 (suppressor of RNA polymerase B, ye AA130633 2.601613 10 2 transmembrane 9 superfamily member 2 AA479100 2.602568 8 4 heat shock 105kD AA485151 2.6112

15 10 6 CDC28 protein kinase 2 AA397813 2.61623 6 2 protein tyrosine kinase 9 AA019459 2.6169615 10 8 Homo sapiens mRNA full length insert cDN H93552 2.6179232 5 4 KIAA0317 gene product AA496541 2.620829 9 5 dickkopf (Xenopus laevis) homolog 3 AA425947 2.6332832 2 8 ESTs R19305 2.637127 10 6 minichromosome maintenance deficient (S. AA488610 2.63808

11 6 4 CDC20 (cell division cycle 20, S. cerevi AA598776 2.6409615 8 5 NADH dehydrogenase (ubiquinone) 1 alpha AA608515 2.6476817 14 4 regulatory factor X, 5 (influences HLA c AA418045 2.6476827 8 1 retinoblastoma-like 2 (p130) N50554 2.6505618 10 6 hypothetical protein H58119 2.6524815 9 2 high-mobility group (nonhistone chromoso H93087 2.658248 8 3 UV radiation resistance associated gene AA490501 2.65921 5 8 hypothetical protein STRAIT11499 R91137 2.66304

25 8 4 preferentially expressed antigen in mela AA598817 2.6630427 13 7 Homo sapiens cDNA: FLJ22854 fis, clone K T77847 2.66418 8 11 ESTs H73608 2.6678418 14 12 ESTs R63694 2.6707221 14 2 proteasome (prosome, macropain) 26S subu AA424503 2.672641 13 7 Homo sapiens mRNA; cDNA DKFZp586C1723 (f H17336 2.680324 14 8 ESTs R00492 2.68128

25 7 9 cullin 3 R27581 2.6851232 5 3 SON DNA binding protein AA431849 2.6908819 1 7 Homo sapiens clone 23568, 23621, 23795, R76529 | R762 2.693767 11 2 syntaxin binding protein 3 R20770 2.698569 7 4 mitogen-activated protein kinase kinase AA053674 2.70432

19 9 1 protein phosphatase 2, regulatory subuni W35378 2.7062412 9 2 ataxin 2 related protein AA029964 2.7177628 2 7 hypothetical protein FLJ23467 R01167 2.7177614 11 12 ATP binding protein associated with cell AA085749 2.721631 11 8 Homo sapiens cDNA: FLJ23156 fis, clone L N76045 2.7244829 8 3 insulin-like growth factor 2 (somatomedi N54596 2.7283223 2 9 hypothetical protein DKFZp547A023 R33219 2.7302428 3 4 RAE1 (RNA export 1, S.pombe) homolog AA504128 2.7331229 13 3 UNK T81033 2.74089 12 1 protease, serine, 2 (trypsin 2) AA284528 2.74752

14 5 3 small nuclear ribonucleoprotein polypept AA521249 2.7542410 3 3 prostaglandin E receptor 4 (subtype EP4) AA019996 2.7609615 5 5 general transcription factor IIA, 2 (12k T55801 2.7686417 13 1 proprotein convertase subtilisin/kexin t R42630 2.7686419 6 6 prefoldin 5 AA446453 2.7705627 5 4 histidine triad nucleotide-binding prote T57556 2.7705632 4 6 ESTs, Weakly similar to I38588 reverse t H94050 2.77056


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22 14 7 olfactory receptor, family 2, subfamily N73548 2.7715212 11 4 HSPC022 protein AA521232 2.7724822 4 12 integral membrane protein 2B AA453275 2.7916814 7 5 serine/threonine protein phosphatase cat AA521083 2.792641 14 6 forkhead box M1 AA136566 2.7936

24 4 12 acetylserotonin O-methyltransferase-like AA427398 2.8089630 13 1 matrin 3 AA075307 2.809926 11 12 MORF-related gene 15 T90438 2.81856

29 13 2 2,4-dienoyl CoA reductase 1, mitochondri H72937 2.819521 12 3 deleted in oral cancer (mouse, homolog) R78608 | R786 2.82336

21 14 4 X-ray repair complementing defective rep R39148 2.8252825 10 11 Homo sapiens cDNA: FLJ22281 fis, clone H AA043944 2.8329614 8 4 solute carrier family 23 (nucleobase tra AA461071 2.841619 9 8 Homo sapiens cDNA FLJ14028 fis, clone HE AA062814 2.8435211 14 2 KIAA0156 gene product R41779 2.8531218 11 11 zinc finger protein 281 N47469 2.8627212 12 1 syndecan binding protein (syntenin) AA456109 2.8675212 12 4 runt-related transcription factor 1 (acu AA425746 2.8684822 14 1 lysosomal-associated membrane protein 2 N62847 2.8694419 5 4 holocytochrome c synthase (cytochrome c AA281549 2.87525 10 10 hypothetical protein MGC5297 R25389 2.878085 3 1 POM (POM121 rat homolog) and ZP3 fusion AA425602 2.883847 1 1 S100 calcium-binding protein A11 (calgiz AA464731 2.883841 9 10 ESTs W56371 2.88864

29 13 6 PTK2 protein tyrosine kinase 2 AA291486 2.8915232 1 3 T-cell acute lymphocytic leukemia 1 T51236 2.913611 8 1 v-ral simian leukemia viral oncogene hom H94892 2.920326 3 2 myeloid/lymphoid or mixed-lineage leukem T54462 2.92128

25 14 5 transmembrane protein with EGF-like and AA431475 2.9212815 5 4 laminin, beta 1 AA446251 2.923226 2 5 reticulocalbin 2, EF-hand calcium bindin AA598676 2.924169 9 5 UNK T62084 2.92608

13 14 6 methionine aminopeptidase; eIF-2-associa AA487589 2.9289610 7 4 heterogeneous nuclear ribonucleoprotein AA599176 2.9318419 7 2 KIAA0365 gene product AA485383 2.9337611 10 1 UNK W68141 2.9347219 8 4 nuclear autoantigen AA418821 2.9347218 12 6 high-glucose-regulated protein 8 N70528 2.937632 6 1 nuclear receptor interacting protein 1 AA458503 2.942418 13 6 nuclear transcription factor Y, beta AA130846 2.9491223 11 5 ornithine aminotransferase (gyrate atrop AA446820 2.9510419 8 5 protein C receptor, endothelial (EPCR) T47443 2.9525 11 7 ESTs R96358 2.959687 11 10 hypothetical protein FLJ20333 R27552 2.9616

14 5 8 deiodinase, iodothyronine, type II R62138 2.9625618 7 7 ribosomal protein L30 N54932 2.966423 10 5 pleiomorphic adenoma gene-like 1 AA463204 2.9769622 3 2 nuclear DNA-binding protein N22178 2.9836827 14 7 RAB2, member RAS oncogene family T82415 2.99046 9 1 ESTs R33154 2.99136

27 9 4 tumor necrosis factor receptor superfami H98636 2.9923214 7 4 vesicle docking protein p115 AA504342 2.99328


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24 1 12 CGI-121 protein W02072 | AA2 2.9980811 3 1 SHB adaptor protein (a Src homology 2 pr AA427595 327 2 1 tousled-like kinase 1 AA113347 3.005768 14 6 MHC class I region ORF T58146 3.00768

24 3 3 succinate dehydrogenase complex, subunit AA463510 3.0076820 10 3 glucose-6-phosphate dehydrogenase AA424938 3.0134426 1 6 DKFZP586D2223 protein H82950 3.013448 6 2 basic transcription element binding prot N80235 3.01441 13 4 basic transcription factor 3 R83000 3.01824

24 8 4 growth factor receptor-bound protein 10 AA136336 3.019226 2 8 HSPC145 protein R22949 3.0249628 7 7 guanine nucleotide binding protein (G pr H72086 3.0259231 2 9 hypothetical protein FLJ20159 R33122 3.0307223 14 4 cytochrome P450, subfamily IIIA (niphedi R91078 3.0326431 8 12 ESTs R14858 3.0489610 8 9 PEST-containing nuclear protein R68102 3.0556822 11 12 CGI-19 protein N91539 3.0691227 8 2 KIAA0307 gene product AA019748 3.0796817 10 6 C-terminal binding protein 1 AA478268 3.081631 10 8 aldo-keto reductase family 1, member B1 R89358 3.0825618 8 10 solute carrier family 18 (vesicular mono N49899 3.0902414 3 8 ESTs R11208 3.0998431 11 10 midline 1 (Opitz/BBB syndrome) R26744 3.1065612 8 7 trinucleotide repeat containing 3 N57754 3.1132827 11 3 lysophospholipase I H01205 3.113289 6 1 UNK W15277 3.123841 14 4 basonuclin R26526 3.1248

14 8 5 coatomer protein complex, subunit beta AA598868 3.1574430 2 6 TRK-fused gene R60847 3.158412 3 3 poly(A)-binding protein, cytoplasmic 1 AA486531 3.166088 11 6 hypothetical protein FLJ10357 R34323 3.168

25 9 6 MAD (mothers against decapentaplegic, Dr AA456439 3.1737630 3 11 ubiquitination factor E4B (homologous to H56031 3.1785628 3 2 eukaryotic translation initiation factor AA194246 3.19228 5 4 interferon, gamma-inducible protein 16 AA490996 3.1958422 8 11 transferrin receptor (p90, CD71) AA029889 3.200644 9 4 DnaJ (Hsp40) homolog, subfamily C, membe T60163 3.203521 10 2 KIAA0308 protein R81782 3.21216

32 4 4 KIAA0332 protein AA459866 3.2236817 8 6 KIAA0191 protein AA449736 3.230415 1 1 calponin 1, basic, smooth muscle AA398400 3.2313619 9 3 c-myc binding protein AA020001 3.2409619 10 3 cyclin G2 AA489647 3.244818 8 3 cleavage and polyadenylation specific fa R44870 3.2476819 8 3 cytochrome c oxidase subunit VIIa polype R10896 3.2486431 11 4 aldo-keto reductase family 1, member C1 R93124 3.2572825 12 4 mannan-binding lectin serine protease 1 H81200 3.263041 11 4 CD47 antigen (Rh-related antigen, integr AA455448 3.28704

29 14 5 NRAS-related gene AA490039 3.3052822 14 4 transforming growth factor, beta recepto AA487034 3.3254421 10 6 cytochrome c oxidase subunit VIc AA456931 3.3283224 4 2 nucleosome assembly protein 1-like 1 R93829 3.32928


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21 11 8 Homo sapiens protein phosphatase 4 regul N64474 3.331223 8 5 nitrogen fixation cluster-like T55560 3.3331210 8 5 esterase D/formylglutathione hydrolase T64482 3.33610 10 6 ESTs, Weakly similar to AF187016 1 myosi H48099 3.3398423 11 8 anaphase-promoting complex subunit 7 T67474 3.352324 11 3 Gardner-Rasheed feline sarcoma viral (v- AA256172 3.36481 8 1 replication protein A3 (14kD) H59259 3.38496

18 11 12 U6 snRNA-associated Sm-like protein AA453273 3.39364 6 8 hypothetical protein R63735 3.39648

19 2 6 ribosomal protein L5 AA496880 3.4003222 7 5 serum/glucocorticoid regulated kinase AA486082 3.4051232 8 11 cathepsin O N81036 3.409928 7 4 mitogen-activated protein kinase kinase AA402447 3.43392

22 14 2 Deleted in split-hand/split-foot 1 regio H85464 3.4396829 10 12 RAB2, member RAS oncogene family R28020 3.45127 10 7 5'-3' exoribonuclease 2 AA035728 3.45312

23 2 7 GL004 protein W76342 3.4713612 13 2 smg GDS-ASSOCIATED PROTEIN H16824 3.4742425 11 2 WAS protein family, member 1 N59851 3.4876819 2 5 COP9 homolog AA489699 3.4934427 12 4 general transcription factor IIH, polype AA455004 3.5059210 12 1 stanniocalcin 1 AA085319 3.516489 14 2 KIAA0203 gene product R38102 3.52704

13 7 4 keratin 5 (epidermolysis bullosa simplex AA160507 3.5683213 11 12 ESTs R69564 3.5836819 8 6 v-jun avian sarcoma virus 17 oncogene ho W96155 3.5865629 11 8 hypothetical protein FLJ10563 R63198 3.600965 10 3 KH-type splicing regulatory protein (FUS T99639 3.610563 8 4 high-mobility group (nonhistone chromoso R53889 3.62016

32 4 1 proteasome (prosome, macropain) subunit, AA047338 3.6201632 1 4 hydroxyacyl-Coenzyme A dehydrogenase/3-k T69767 3.6326432 6 3 SET translocation (myeloid leukemia-asso AA608548 3.6748829 7 3 hypothetical protein 24636 N62562 3.6806429 10 3 G protein-coupled receptor 19 H07878 3.6940822 7 2 ADP-ribosylation factor-like 6 interacti H20558 3.7036832 1 10 Homo sapiens cDNA: FLJ21564 fis, clone C N74925 3.7084825 8 3 transcription factor 7-like 2 (T-cell sp AA419628 3.713289 8 12 butyrate response factor 1 (EGF-response AA723035 3.74208

18 14 4 RAB2, member RAS oncogene family T82415 3.7641628 4 12 UNK AA027317 3.7708825 12 1 proteasome (prosome, macropain) subunit, R27585 3.7718418 11 4 ribosomal protein L32 R43544 3.7987232 2 1 Homo sapiens cDNA: FLJ21930 fis, clone H AA424516 3.8073611 8 12 seven in absentia (Drosophila) homolog 1 AA447531 3.8092823 11 7 zinc finger protein H53869 3.809288 2 6 Homo sapiens, Similar to RIKEN cDNA 5430 R00846 3.82081 2 3 annexin A8 AA235002 3.83136

23 6 12 MCT-1 protein R70784 3.8410 11 7 developmentally regulated GTP-binding pr AA488336 3.853445 11 1 KIAA1046 protein R78541 | R784 3.8544

22 10 5 Homo sapiens mRNA; cDNA DKFZp564O2363 (f AA410260 3.8726431 14 11 UNK R95882 3.92064


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21 12 1 transcription elongation factor A (SII), H28344 3.974424 7 7 slug (chicken homolog), zinc finger prot H57310 3.983047 9 2 high-mobility group (nonhistone chromoso R43217 3.98592

19 4 1 activin A receptor type II-like 1 H43049 4.0070414 6 5 selenoprotein P, plasma, 1 AA070226 4.0444817 8 12 presenilin 2 (Alzheimer disease 4) AA056325 4.1270425 7 1 ribosomal protein L21 AA464743 4.197126 2 6 S-phase response (cyclin-related) R51052 4.2336

29 11 5 chemokine (C-X-C motif), receptor 4 (fus T62636 4.243232 2 3 syndecan 2 (heparan sulfate proteoglycan H64347 4.257623 4 4 leukotriene A4 hydrolase AA465366 4.2585618 14 2 SWI/SNF related, matrix associated, acti AA478436 4.2758429 8 6 chimerin (chimaerin) 1 AA598668 4.3213 14 2 proteasome (prosome, macropain) subunit, N53065 4.410247 2 6 serine (or cysteine) proteinase inhibito AA410517 4.44672

18 3 2 fibroblast growth factor 1 (acidic) AA015914 4.457284 12 1 ribosomal protein S6 N91584 4.488965 1 6 dUTP pyrophosphatase AA489219 4.5216

18 9 3 butyrate response factor 1 (EGF-response AA424743 4.63221 9 6 cell division cycle 2, G1 to S and G2 to AA598974 4.6790432 2 4 adaptor-related protein complex 3, sigma AA460727 4.875844 6 4 insulin-like growth factor binding prote AA598601 4.903687 8 9 methionine aminopeptidase; eIF-2-associa AA487589 4.922887 5 2 protein tyrosine phosphatase, receptor t R79082 4.944

14 11 4 vimentin AA486321 4.9468831 13 9 UNK T96969 5.009288 11 7 hypothetical protein FLJ10889 T91245 5.11968

31 14 6 reproduction 8 AA465216 5.2483216 12 3 cyclin G1 AA082943 5.2771221 2 4 myristoylated alanine-rich protein kinas AA482231 5.30886 1 1 ubiquitin carrier protein E2-C AA430504 5.34912

23 12 5 nuclear factor of kappa light polypeptid AA451716 5.4489610 3 10 KIAA1560 protein T98163 5.4604831 12 8 CGI-69 protein N68172 5.494084 9 6 zinc finger protein 216 N62343 5.68608

22 5 5 sparc/osteonectin, cwcv and kazal-like d AA436142 5.780168 2 2 S100 calcium-binding protein A2 AA458884 5.80416

31 12 12 GATA-binding protein 2 R32406 5.842561 4 6 metallothionein 1L N80129 5.86944

31 13 8 f-box and leucine-rich repeat protein 5 H68988 5.9212832 1 6 flavin containing monooxygenase 3 H71848 6.1612823 8 2 G protein-coupled receptor N53172 6.355222 3 7 EST, Moderately similar to Cd-7 Metallot R16539 6.435842 4 6 leptin receptor overlapping transcript-l T67103 6.44352

21 2 2 metallothionein 1G H53340 6.4550421 13 2 metastasis associated 1 N71159 6.590415 6 11 ESTs, Weakly similar to T17346 hypotheti N69569 6.663361 8 5 osteoblast specific factor 2 (fasciclin AA598653 7.016647 12 10 UNK R31107 7.04256

13 13 2 ESTs, Highly similar to SMHU1B metalloth H72723 7.231 13 10 ESTs R31575 7.3641632 1 12 UDP-glucose:glycoprotein glucosyltransfe W33165 7.6128


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30 1 6 metallothionein 1H H77597 7.785632 9 2 isopentenyl-diphosphate delta isomerase H08820 7.972814 8 7 LIM and cysteine-rich domains 1 R63811 8.00164 13 5 KIAA0101 gene product W68220 8.09376

29 2 9 ESTs R32297 12.206431 14 13 NAC1 Cont-8 19.6876822 5 6 ESTs N55540 21.54816


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ArteagaYukiko Ueda, Shizhen Wang, Nancy Dumont, Jae Youn Yi, Yasuhiro Koh and Carlos L.

TGF•-induced cell motilityOverexpression of HER2 (erbB2) in human breast epithelial cells unmasks

published online March 24, 2004J. Biol. Chem.

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Overexpression of HER2 (erbB2) in human breast epithelial ... › content › early › 2004 › 03 › 24 › jbc.M400081200.full.pdf · 1 Overexpression of HER2 (erbB2) in human

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