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0 10 20 30 40 50 0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 587 in YP10D + 5mM HMF at 42C 70 mM 60 mM 50 mM 40 mM 30 mM 0 mM Time, h OD600 0 10 20 30 40 50 0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 587 in YP10D+5-HMF at 30C 70 mM 60 mM 50 mM 40 mM 30 mM 0 mM Time, h OD600 0 10 20 30 40 50 0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 587 in YP10D+furfural at 30C 80 mM 70 mM 60 mM 50 mM 40 mM 0 mM Time, h OD600 0 10 20 30 40 50 0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 587 in YP10D+furfural at 42C 80 mM 70 mM 60 mM 50 mM 40 mM 0 mM Time, h OD600 howed same level of inhibition to furfural, when it was compared with TISTR5 87 showed some tolerance to 5-HMF, when it was compared with TISTR5925. Attached files for Editor
4

NCYC587 showed same level of inhibition to furfural, when it was compared with TISTR5925.

Feb 24, 2016

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Attached files for Editor . NCYC587 showed same level of inhibition to furfural, when it was compared with TISTR5925. . NCYC587 showed some tolerance to 5-HMF, when it was compared with TISTR5925. . NCYC587 exhibited the sensitivity to lacti c acid, when it was compared with TISTR5925. . - PowerPoint PPT Presentation
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Page 1: NCYC587 showed same  level of inhibition  to furfural,  when it was compared with TISTR5925.

0 10 20 30 40 500.0

0.20.4

0.6

0.8

1.0

1.21.4

1.6

587 in YP10D + 5mM HMF at 42C

70 mM

60 mM

50 mM

40 mM

30 mM

0 mM

Time, h

OD6

00

0 10 20 30 40 500.0

0.2

0.4

0.6

0.8

1.0

1.2

1.4

1.6

587 in YP10D+5-HMF at 30C

70 mM

60 mM

50 mM

40 mM

30 mM

0 mM

Time, h

OD6

00

0 10 20 30 40 500.0

0.2

0.4

0.6

0.8

1.0

1.2

1.4

1.6

587 in YP10D+furfural at 30C

80 mM

70 mM

60 mM

50 mM

40 mM

0 mM

Time, h

OD6

00

0 10 20 30 40 500.0

0.2

0.4

0.6

0.8

1.0

1.2

1.4

1.6587 in YP10D+furfural at 42C

80 mM

70 mM

60 mM

50 mM

40 mM

0 mM

Time, h

OD6

00

NCYC587 showed same level of inhibition to furfural, when it was compared with TISTR5925.

NCYC587 showed some tolerance to 5-HMF, when it was compared with TISTR5925.

Attached files for Editor

Page 2: NCYC587 showed same  level of inhibition  to furfural,  when it was compared with TISTR5925.

0 10 20 30 40 50 600.0

0.2

0.4

0.6

0.8

1.0

1.2

1.4 587 in YP10D + Lactic acid (30 ) ℃

0mM200mM300mM400mM500mM600mM

Time (h)

A600

0 10 20 30 40 50 600.0

0.2

0.4

0.6

0.8

1.0

1.2

1.4 587 in YP10D + Lactic acid (42 ) ℃

0mM

200mM

300mM

400mM

500mM

600mM

Time (h)

A600

NCYC587 exhibited the sensitivity to lactic acid, when it was compared with TISTR5925.

Page 3: NCYC587 showed same  level of inhibition  to furfural,  when it was compared with TISTR5925.

Incubation with YPD with acetic acid at 30C

NCYC1429NCYC587NCYC2791TISTR5925

10010-110-210-310-4

NCYC1429NCYC587NCYC2791TISTR5925

10010-110-210-310-4

Kluyveromyces marxianus

10010-110-210-310-4 10010-110-210-310-4

S288cK3K7NCYC3233S3

S288cK3K7NCYC3233S3

Saccharomyces cervisiae

Page 4: NCYC587 showed same  level of inhibition  to furfural,  when it was compared with TISTR5925.

Incubation on YPD with acetic acid at 42C

NCYC1429NCYC587NCYC2791TISTR5925

10010-110-210-310-4

NCYC1429NCYC587NCYC2791TISTR5925

10010-110-210-310-4

10010-110-210-310-4

10010-110-210-310-4

S288cK3K7NCYC3233S3

S288cK3K7NCYC3233S3

Kluyveromyces marxianus TISTR5925 strain has more acid tolerance than other strains. Saccharomyces cerevisiae doesn’t show good performance at high temperature.

Saccharomyces cervisiae