mynameis butyoumayalsoknowmeas Hep-2, KB...Now you can speed discovery with the power of multiparameter cell analysis using the best-in-class BD Accuri C6 personal fl cytometer. Never
This document is posted to help you gain knowledge. Please leave a comment to let me know what you think about it! Share it to your friends and learn new things together.
ATCC performs a combination of test methods, including Human STR analysis,COI testing, Mycoplasma testing, Human viral testing, and Sterility testing, onall cell lines to ensure the highest quality and authenticity.
Learn how to avoid cell misidentification, visitwww.atcc.org/trustedsource
Rethink Western blotting.Take protein detectionto new dimensions.
EMD Millipore is a division of Merck KGaA, Darmstadt, Germany
EMD Millipore and the M mark are trademarks of Merck KGaA, Darmstadt, Germany. SNAP i.d. is a registered trademark of Merck KGaA, Darmstadt, Germany.
• ElectroLigase,™ directly compatible with electroporation
Stick together with DNA Ligases and Ligase Master Mixes
from NEB.
To request a FREE SAMPLE of our new DNA Ligase
Master Mixes, visit NEBStickTogether.com
Stick together.
ELECTROLIGASE™ is a trademark of New England Biolabs.
Duplicate ligation reactions of blunt or T/A vector/insert pairs were set up according to themaster mix vendors’ suggestions. Equal amounts of ligated DNA were used to transform NEB10-beta Competent E. coli (NEB #C3019) and triplicate plating was performed. Transformationresults were averaged and graphed as a percentage of the highest performing reaction, T/Aligation using the Blunt/TA Ligase Master Mix.
Blunt/TA Ligase Master Mix outperforms
the competition
0
25
50
75
100
Transformationefficiency(%)
(ascomparedtoBlunt/TALigaseMasterMix)
Company
A
Company
B
Company
C
NEB
Blunt/TA Ligase
Master Mix
T/A ligation
Blunt ligation
www.cellsignal.comfor quality products you can trust...
For Research Use Only. Not For Use In Diagnostic Procedures. Orders (toll-free) 1-877-616-2355 | Inquiries [email protected] | Environmental Commitment eco.cellsignal.com
Jurkat
untreated
inhibitor
cocktail-
treated
Unparalleled product quality, validation, and technical support
webinars, blogs, discounts, and other content geared
for people who aren’t afraid of footnotes.
������� ��������� ��Brought to you by theScience/AAAS Custom
Publishing Office
tional modifications or to monitor the purification process of the proteins produces
a large number of samples that can easily exceed the capacity of modern analytical
laboratories. High throughput analytical platforms with high precision, automa-
tion, and ease-of-use are therefore in great demand. Microfluidic-based assays for
screening protein product quality are finding wide use because they address the
limitations of SDS-PAGE as well as other separation assays that depend on conven-
tional capillary electrophoresis. These assays leverage microfluidic technology to
reduce analysis times dramatically compared with conventional techniques, to a
minute or less per sample. In this presentation we will discuss the use of microfluid-
ic-CE platforms for characterization assays such as purity assessment of monoclo-
nal antibodies under reducing and nonreducing conditions, N-glycan profiling, and
determination of protein charge heterogeneity.
����� ��� ������� ��� ������ ��
• Learn about the pros and cons of using microfluidics for high throughputprotein analysis
• Obtain expert advice on setting up and validating microfluidics-based assays
• Compare results obtained using microfluidics assays to conventional methods
• Have their questions answered live during the broadcast!
Therapeutic proteins are large complex molecules that are often heterogeneous
in molecular structures. The control and characterization of biotherapeutic protein
quality throughout the development process has been a major focus of the biotech-
nology industry. Studies to test the effect of cell culture conditions on posttransla-
tional modifications or to monitor the purification process of the proteins produces
Wednesday, November 14, 201212 noon ET, 9 a.m. PT, 4 p.m. UK, 5 p.m. CET
Microfluidic Electrophoresis Assaysfor Rapid Characterization of Proteinin Research and Development
�����������
���� ������� �����
���������� ������� ����� �
� ��� �
��������� �����
��� ���� �����
����� ������
����������� ��
����� ��������� �����
���������
��� ��������� ��
�������� ��
���������������������
WEBINAR
Personal Flow Cytometry from BD BiosciencesBD Pharmingen™ reagents and BD Accuri™ C6
Now you can speed discovery with the power ofmultiparameter cell analysis using the best-in-class BD Accuri™ C6 personal flow cytometer.Never before have the power and insight ofpersonal flow cytometry been more within reach.Today, you can take advantage of anunprecedented value package including 10%savings on the BD Accuri C6 and 40% savings onthe high quality BD reagents you’ll use with it fora full two years.
With this package, you get 4-color cell analysisin an affordable, transportable, and easy-to-use
format that serves both novice and experiencedresearchers well, right from the benchtop.
The software’s intuitive interface guides youthrough workflows, making it easy to begincollecting and analyzing data—even if youhave little flow cytometry know-how. Setupand maintenance are also simplified to increaseavailability and up-time.
Take advantage of this value package todayat bdbiosciences.com/go/accuri.
Announcing our new partnership with NASA Federal Credit Union
Dear Member:
AAAS is committed to ofering you member benefts that ft your needs and make your membership
more valuable.
With that in mind AAAS is embarking on a new partnership with NASA Federal Credit Union that will
provide you with access to a wide range of fnancial tools and products. Like AAAS, NASA Federal Credit
Union is dedicated to serving the scientifc community. This shared perspective is just one of the many
reasons that we are embarking on this partnership.
As you may know, we have recently ended our banking relationship with Bank of America, but we’re
confdent that our new partnership with NASA FCU will provide you with a superior banking experience.
NASA FCU ofers members better ways to save and smarter ways to borrow with friendly, professional
service – along with anytime, anywhere account access.
Moreover, unlike other fnancial institutions that have public stockholders, NASA FCU is a not-for-proft
fnancial cooperative where being a member means being an owner, too. And as a member/owner,
you will enjoy unique benefts like: better loan rates, higher dividends and state-of-the-art products and
services.
We’ll be sending you more information about this great new beneft over the coming months. In the
meantime, be sure to visit nasafcu.com/AAASpackage to apply for the new AAAS Platinum Advantage
Rewards or Platinum Cash Rewards credit cards. You can also take a sneak peek at the AAAS Check Card
and Checks coming soon.
Sincerely,
Ian King
Director of Marketing and Membership, AAAS
New Products: General Lab Equipment
Pr Produoducedced by by th thee SciSciencencee/AA/AAASAS CusCustomtom Pu Publiblishishingng OffOfficeice LLIIFFEE SSCCIIEENNCCEE TTEECCHHNNOOLLOOGGIIEESS
Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines in academic, industrial, and governmental organizations are
featured in this space. Emphasis is given to purpose, chief characteristics, and availability of products and materials. Endorsement by Science or AAAS of any products or
materials mentioned is not implied. Additional information may be obtained from the manufacturer or supplier.
Electronically submit your new product description or product literature information! Go to www.sciencemag.org/products/newproducts.dtl for more information.
for its generous support of the Science Journalism Awards
Register Today at Discounted RatesHousingSpecial room rates and travel benefits are available toAnnual Meeting registrants.
Sheraton Boston HotelRate: $205 single/double
Hilton Back BayRate: $197 single/ $207 double
Boston Marriott Copley PlaceRate: $192 single/ $208 double
Rooms are available on a first-come, first-served basisuntil 21 January 2013.
AAAS, publisher of Science, thanks the sponsors and
supporters of the 2013 Annual Meeting
RegistrationDiscounted advance registration rates are available untilMonday, 21 January 2013.
Take advantage of unlimited access to all symposia,seminars, topical lectures, plenary events, career workshops,the Exhibit Hall, and a variety of networking opportunities.
Professional$295 Member/ $375 New Member/ $399 Non-Member
Postdoc$235 Member/ $315 New Member/ $335 Non-Member
K-12 Teacher$235 Member/ $315 New Member/ $355 Non-Member
Emeritus$235 Member/ $315 New Member/ $355 Non-Member
Student$60 Member/ $70 New Member/ $90 Non-Member
After 21 January 2013, on-site rates apply.
For more information, visit www.aaas.org/meetings 1 4 - 1 8 F E B R U A R Y • B O S TO N
ANNUALMEETING
2013AAAS
Make a Difference.
Help give science a greater voice inWashington, DC!
Since 1973, AAAS Fellows have applied their skills to
federal decision-making processes that affect people
in the U.S. and around the world, while learning first-
hand about the government and policymaking.
Join the Network.
Year-long fellowships are available in theU.S.
Congress and federal agencies. Applicants must
hold a PhD or equivalent doctoral-level degree in
any behavioral/social, biological, computational/
mathematical, earth, medical/health, or physical
science, or any engineering discipline. Individuals
with amaster’s degree in engineering and three
years of professional experience also may apply.
Federal employees are not eligible and U.S.
citizenship is required.
Apply.
The application deadline for the 2013-2014
AAASScience&Technology Policy Fellowships
is 5December. Fellowships are awarded in the
spring andbegin in September. Stipends range
from$74,000 to $97,000.
Note: Additional fellowships are available
through approximately 30 scientific society
partners. Individuals are encouraged to apply
with AAAS as well as with any scientific societies
for which they qualify.
Full details at: fellowships.aaas.org
Picture yourself as a
AAAS Science & Technology
Policy Fellow
SabrinaMcCormick, PhD
Sociology and Environmental
Sciences, Brown University
2009-11 S&T Policy Fellow,
Environmental Protection
Agency, Office of Research
and Development
Now president,
Evidence BasedMedia
Enhancing Public Policy,
Advancing Science Careers
SCIENCE & TECHNOLOGY
POLICY FELLOWSHIPS
For experimental details, additionalinformation, and a complete list of availableXP® monoclonal antibodies visit…