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Your trusted source since 1925 ©2012 American Type Culture Collection. ATCC® is a registered trademark and the ATCC logo is a trademark of the American Type Culture Collection. ATCC products are intended for laboratory research only. They are not intended for use in humans, animals, or diagnostics. Hi my name is HeLa but you may also know me as Hep-2, or even as KB ATCC performs a combination of test methods, including Human STR analysis, COI testing, Mycoplasma testing, Human viral testing, and Sterility testing, on all cell lines to ensure the highest quality and authenticity. Learn how to avoid cell misidentification, visit www.atcc.org/trustedsource
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Page 1: mynameis butyoumayalsoknowmeas Hep-2, KB...Now you can speed discovery with the power of multiparameter cell analysis using the best-in-class BD Accuri C6 personal fl cytometer. Never

Your trusted source since 1925

©2012 American Type Culture Collection. ATCC® is a registered trademark and the ATCC logo is a trademark of the American Type Culture Collection. ATCC products are intended forlaboratory research only. They are not intended for use in humans, animals, or diagnostics.

Hi

my name is HeLa

but you may also knowme as Hep-2, or even as KB

ATCC performs a combination of test methods, including Human STR analysis,COI testing, Mycoplasma testing, Human viral testing, and Sterility testing, onall cell lines to ensure the highest quality and authenticity.

Learn how to avoid cell misidentification, visitwww.atcc.org/trustedsource

Page 2: mynameis butyoumayalsoknowmeas Hep-2, KB...Now you can speed discovery with the power of multiparameter cell analysis using the best-in-class BD Accuri C6 personal fl cytometer. Never

Rethink Western blotting.Take protein detectionto new dimensions.

EMD Millipore is a division of Merck KGaA, Darmstadt, Germany

EMD Millipore and the M mark are trademarks of Merck KGaA, Darmstadt, Germany. SNAP i.d. is a registered trademark of Merck KGaA, Darmstadt, Germany.

© 2012 EMD Millipore Corporation, Billerica, MA USA. All rights reserved.

Biology isn’t flat—neither are your Westerns.

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drive antibodies and buffers right through the membrane.

This advanced technology promotes antigen binding and

thorough washing, enabling you to better optimize your

Western blotting conditions.

www.emdmillipore.com/SNAP

Page 3: mynameis butyoumayalsoknowmeas Hep-2, KB...Now you can speed discovery with the power of multiparameter cell analysis using the best-in-class BD Accuri C6 personal fl cytometer. Never

New DNA Ligases and Ligase Master MixesNew England Biolabs offers the most extensive selection of

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Duplicate ligation reactions of blunt or T/A vector/insert pairs were set up according to themaster mix vendors’ suggestions. Equal amounts of ligated DNA were used to transform NEB10-beta Competent E. coli (NEB #C3019) and triplicate plating was performed. Transformationresults were averaged and graphed as a percentage of the highest performing reaction, T/Aligation using the Blunt/TA Ligase Master Mix.

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Page 4: mynameis butyoumayalsoknowmeas Hep-2, KB...Now you can speed discovery with the power of multiparameter cell analysis using the best-in-class BD Accuri C6 personal fl cytometer. Never

www.cellsignal.comfor quality products you can trust...

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Page 5: mynameis butyoumayalsoknowmeas Hep-2, KB...Now you can speed discovery with the power of multiparameter cell analysis using the best-in-class BD Accuri C6 personal fl cytometer. Never

The moment your data change

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Page 6: mynameis butyoumayalsoknowmeas Hep-2, KB...Now you can speed discovery with the power of multiparameter cell analysis using the best-in-class BD Accuri C6 personal fl cytometer. Never

Discover more at abcam.com/episeeker

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Page 7: mynameis butyoumayalsoknowmeas Hep-2, KB...Now you can speed discovery with the power of multiparameter cell analysis using the best-in-class BD Accuri C6 personal fl cytometer. Never

NanoDrop™ 2000Full-spectrum microvolumemeasurements

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Page 8: mynameis butyoumayalsoknowmeas Hep-2, KB...Now you can speed discovery with the power of multiparameter cell analysis using the best-in-class BD Accuri C6 personal fl cytometer. Never

membercentral.aaas.org

58,905polysyllabic words

reexaminingArdipithecus ramidus.

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Page 9: mynameis butyoumayalsoknowmeas Hep-2, KB...Now you can speed discovery with the power of multiparameter cell analysis using the best-in-class BD Accuri C6 personal fl cytometer. Never

������� ��������� ��Brought to you by theScience/AAAS Custom

Publishing Office

tional modifications or to monitor the purification process of the proteins produces

a large number of samples that can easily exceed the capacity of modern analytical

laboratories. High throughput analytical platforms with high precision, automa-

tion, and ease-of-use are therefore in great demand. Microfluidic-based assays for

screening protein product quality are finding wide use because they address the

limitations of SDS-PAGE as well as other separation assays that depend on conven-

tional capillary electrophoresis. These assays leverage microfluidic technology to

reduce analysis times dramatically compared with conventional techniques, to a

minute or less per sample. In this presentation we will discuss the use of microfluid-

ic-CE platforms for characterization assays such as purity assessment of monoclo-

nal antibodies under reducing and nonreducing conditions, N-glycan profiling, and

determination of protein charge heterogeneity.

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• Learn about the pros and cons of using microfluidics for high throughputprotein analysis

• Obtain expert advice on setting up and validating microfluidics-based assays

• Compare results obtained using microfluidics assays to conventional methods

• Have their questions answered live during the broadcast!

Therapeutic proteins are large complex molecules that are often heterogeneous

in molecular structures. The control and characterization of biotherapeutic protein

quality throughout the development process has been a major focus of the biotech-

nology industry. Studies to test the effect of cell culture conditions on posttransla-

tional modifications or to monitor the purification process of the proteins produces

Wednesday, November 14, 201212 noon ET, 9 a.m. PT, 4 p.m. UK, 5 p.m. CET

Microfluidic Electrophoresis Assaysfor Rapid Characterization of Proteinin Research and Development

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Page 10: mynameis butyoumayalsoknowmeas Hep-2, KB...Now you can speed discovery with the power of multiparameter cell analysis using the best-in-class BD Accuri C6 personal fl cytometer. Never

Personal Flow Cytometry from BD BiosciencesBD Pharmingen™ reagents and BD Accuri™ C6

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Page 11: mynameis butyoumayalsoknowmeas Hep-2, KB...Now you can speed discovery with the power of multiparameter cell analysis using the best-in-class BD Accuri C6 personal fl cytometer. Never

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Page 12: mynameis butyoumayalsoknowmeas Hep-2, KB...Now you can speed discovery with the power of multiparameter cell analysis using the best-in-class BD Accuri C6 personal fl cytometer. Never

Announcing our new partnership with NASA Federal Credit Union

Dear Member:

AAAS is committed to ofering you member benefts that ft your needs and make your membership

more valuable.

With that in mind AAAS is embarking on a new partnership with NASA Federal Credit Union that will

provide you with access to a wide range of fnancial tools and products. Like AAAS, NASA Federal Credit

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We’ll be sending you more information about this great new beneft over the coming months. In the

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and Checks coming soon.

Sincerely,

Ian King

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Page 13: mynameis butyoumayalsoknowmeas Hep-2, KB...Now you can speed discovery with the power of multiparameter cell analysis using the best-in-class BD Accuri C6 personal fl cytometer. Never

New Products: General Lab Equipment

Pr Produoducedced by by th thee SciSciencencee/AA/AAASAS CusCustomtom Pu Publiblishishingng OffOfficeice LLIIFFEE SSCCIIEENNCCEE TTEECCHHNNOOLLOOGGIIEESS

Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines in academic, industrial, and governmental organizations are

featured in this space. Emphasis is given to purpose, chief characteristics, and availability of products and materials. Endorsement by Science or AAAS of any products or

materials mentioned is not implied. Additional information may be obtained from the manufacturer or supplier.

Electronically submit your new product description or product literature information! Go to www.sciencemag.org/products/newproducts.dtl for more information.

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687www.sciencemag.org/products SCIENCE VOL 338 2 NOVEMBER 2012

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Page 14: mynameis butyoumayalsoknowmeas Hep-2, KB...Now you can speed discovery with the power of multiparameter cell analysis using the best-in-class BD Accuri C6 personal fl cytometer. Never

AAAS thanks

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Make a Difference.

Help give science a greater voice inWashington, DC!

Since 1973, AAAS Fellows have applied their skills to

federal decision-making processes that affect people

in the U.S. and around the world, while learning first-

hand about the government and policymaking.

Join the Network.

Year-long fellowships are available in theU.S.

Congress and federal agencies. Applicants must

hold a PhD or equivalent doctoral-level degree in

any behavioral/social, biological, computational/

mathematical, earth, medical/health, or physical

science, or any engineering discipline. Individuals

with amaster’s degree in engineering and three

years of professional experience also may apply.

Federal employees are not eligible and U.S.

citizenship is required.

Apply.

The application deadline for the 2013-2014

AAASScience&Technology Policy Fellowships

is 5December. Fellowships are awarded in the

spring andbegin in September. Stipends range

from$74,000 to $97,000.

Note: Additional fellowships are available

through approximately 30 scientific society

partners. Individuals are encouraged to apply

with AAAS as well as with any scientific societies

for which they qualify.

Full details at: fellowships.aaas.org

Picture yourself as a

AAAS Science & Technology

Policy Fellow

SabrinaMcCormick, PhD

Sociology and Environmental

Sciences, Brown University

2009-11 S&T Policy Fellow,

Environmental Protection

Agency, Office of Research

and Development

Now president,

Evidence BasedMedia

Enhancing Public Policy,

Advancing Science Careers

SCIENCE & TECHNOLOGY

POLICY FELLOWSHIPS

Page 16: mynameis butyoumayalsoknowmeas Hep-2, KB...Now you can speed discovery with the power of multiparameter cell analysis using the best-in-class BD Accuri C6 personal fl cytometer. Never

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