MYCOBACTERIUM TUBERCULOSIS PROTEOME • M. tuberculosis- intracellular pathogen • - TB prevalent in Africa and Asia • - 1/3 population is infected • - 8 million new cases per year • Complete sequence is available in SPTR: • NCBI_TaxID=1773 (Rv=83332) (CDC1551=83331) • 4087 proteins in SPTR, 2706 are “hypothetical” • 66% of proteins in SPTR uncharacterised
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MYCOBACTERIUM TUBERCULOSIS PROTEOME M. tuberculosis- intracellular pathogen - TB prevalent in Africa and Asia - 1/3 population is infected - 8 million.
Proteome analysis of Mtb Extensive gene duplication, functional redundancy and diversification Precise function 40%, some functional info 44%, 16% unknown, probably Mycobacterial specific 9% genome encodes PE and PPE proteins (gly rich) Many fatty acid metabolism proteins >250 cf 50 in E.coli (reflection of cell wall) Has two hemoglobins for oxygen storage Mycobactins for iron Glyoxylate shunt NB for latency or persistence (isocitrate lyase)
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MYCOBACTERIUM TUBERCULOSIS PROTEOME
• M. tuberculosis- intracellular pathogen • - TB prevalent in Africa and Asia• - 1/3 population is infected • - 8 million new cases per year• Complete sequence is available in SPTR:• NCBI_TaxID=1773 (Rv=83332) (CDC1551=83331)• 4087 proteins in SPTR, 2706 are “hypothetical”• 66% of proteins in SPTR uncharacterised
- 2505 have InterPro matches (64.7%)- Of those in InterPro, at least 860 hypothetical can be characterised- 33% of hypothetical proteins in SPTR, or 22% proteome
Proteome analysis of Mtb
• Extensive gene duplication, functional redundancy and diversification
• Precise function 40%, some functional info 44%, 16% unknown, probably Mycobacterial specific
• 9% genome encodes PE and PPE proteins (gly rich)• Many fatty acid metabolism proteins >250 cf 50 in E.coli
(reflection of cell wall)• Has two hemoglobins for oxygen storage• Mycobactins for iron• Glyoxylate shunt NB for latency or persistence (isocitrate
lyase)
Structural information
• 1079 ORFs can be assigned to SCOP -1566 domains, 1639 regions
• 482 (12.4%) have hits in HSSP• 9 (0.2%) have hits in PDB
Venn diagram of InterPro hits for the 3 organisms
E.coli
M.tuberB. sub
675
68125
62
129
76
59
Protein secretion systemFlagellar proteinsGram -ve porinsLypolytic enzymesSpecific transposases