Microscope type? SEM TEM CLM - MCCCblinderl/documents/unit2reviewstudents.pdf · Microscope type? SEM TEM CLM Cheek cell Uses electrons Uses light Surface structures Pollen Internal

Microscope type?SEM TEM CLM

Cheek cell Uses electrons

Uses light

Surface structures

Pollen Internal structures

Mag. Up to 1000X

Mag. 20,000X

Virus

Centrifugation

• Homogenate, supernatant, pellet

Homogenization

Homogenate

Differential centrifugation

Tissuecells

Prokaryotic or Eukaryotic or both

• Nucleoid region• Cell wall• Flagella• DNA• High surface/volume ratio• Nuclear envelope• Single celled• Bacteria• Fermentation• Cytoplasm• Plasma membrane and phospholipid bilayer• Mitochondria• Glycolysis• Oxidative phosphorylation

Active or passive process?

• Diffusion

• Osmosis

• Endocytosis

• Exocytosis

• Pinocytosis

• Phagocytosis

• Facilitated diffusion

• Sodium/ Potassium pump

What is the significance of these proteins?• Integral protein• Peripheral protein• Transmembrane protein• Channel protein• Carrier protein• Glucose transporter• Enzyme• Lysosomal enzyme• Catalase• Microtubules• Microfilaments• Aquaporin• Na/K pump• ATP synthase• Electron transport proteins• Glycolysis Enzymes• Citric Acid Cycle enzymes

What are these types of molecules or substances?

• H2O2

• Cholesterol• Phospholipid• Chromosome• Inorganic cofactor• ATP• ADP• Pyruvate• Glucose• Acetyl CoA• H+• NADH and FADH2

Build a phospholipid bilayer

• Channel protein

• Phospholipids– Hydrophilic heads

– Hydrophobic tails

• Integral protein

• Peripheral protein

• Carrier protein

• Amphipathic protein

• Cholesterol

What process? How do you know?