Microbial Production of Methanol 1 Presented By: Mohsin Shad Registration Number: 2013-BC-005 Date: 18-04-2016 Presented BY: Dr. Muhammad Imran
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Microbial Production of Methanol
Presented By: Mohsin ShadRegistration Number: 2013-BC-005Date: 18-04-2016Presented BY: Dr. Muhammad Imran
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Methanol
Methanol, also known as Methyl Alcohol Methanol is the simplest alcohol (CH3OH) Methyl group linked to a Hydroxyl group Light, volatile, colorless, flammable liquid Methanol is highly toxic and unfit for
consumption.
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Microbial Production of Methanol
Methanol is Produced By: (1) Yeast (2) Fungi (3) Bacteria Microbes produce Pectin Methyl Esterase PME Produce Methanol from Fruits And Juices Microbes can also produce methanol via the
oxidation of methane (biogas)
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Pectin
Structural Hetero-polysaccharide Gelling agent in food Source of dietary fiber Stabilizer in fruit juices and milk drinks Methanol is produced by fermentation of fruits Pectin Methyl Esterase (PME) PME produced by Microorganism
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Production of Methanol by Pectin
Pectin enzymes are produced by yeast, bacteria, fungi and plants
Proteolytic enzymes are classified into: (1) Esterases (2) Depolymerase (lyases and hydrolyses) lyases produces oligo- or mono-galacturonate Hydrolysis of pectin by esterases produces pectic
acid and methanol
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Production of Methanol by Pectin
PME hydrolyses pectin to poly-galactouronic acid and methanol
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Methane Monooxygenase
PMMO – Particulate Methane Monooxygenase SMMO – Soluble Methane Monoxygenase
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Advantages of using Ammonia-Oxidizing Bacteria
Oxidize methane to methanol via the nonspecific
action of the enzyme ammonia monooxygenase
Contaminants such as moisture and CO2 do not
post a limitation for biological conversion
Can utilize the CO2 contained in gas mixtures for
cell synthesis
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Metabolism of AOB
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Methanol Production by AOB
Methanol production rate varies for conditions and
performance
Maximum specific productivity is 0.82 mg
methanol/ mg biomass (COD)/d
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Uses of Methanol
Methanol is used in: Fuel Antifreeze Cleaners Canned Heat Paints, Paint Thinner and Removers
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