Method-Specific Sample Preparation for EPH Analysis · Method-Specific Sample Preparation for EPH Analysis Resprep SPE Cartridges for Complete Extractable Petroleum Hydrocarbon Fractionation

Method-Specific Sample Preparation for EPH AnalysisResprep SPE Cartridges for Complete Extractable Petroleum Hydrocarbon Fractionation

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• Full separation between fractions.

• Guaranteed low background level.

• Superior lot-to-lot reproducibility.

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Figure 1: Resprep EPH SPE cartridges offer consistent and complete separation between aliphatic and aromatic compounds from one lot to the next. A representative subset of compounds is shown here (40+ compounds were analyzed in total) to clearly illustrate the separation between fractions and the lack of breakthrough in every lot we produce.

• Method-specific performance for extractable petroleum hydrocarbon (EPH) analysis of soil and water samples.

• Complete separation of aliphatic and aromatic compounds into distinct fractions.

• Guaranteed background level under the strict reporting limits of MA and NJ EPH methods.

• Superior lot-to-lot reproducibility and storage stability ensured by rigorous QC testing and moisture-resistant packaging.

Resprep EPH fractionation SPE cartridges from Restek are manufactured in a strictly controlled environment with the simple goal of giving you consistent results—every day, every time. Turn to Resprep cartridges for easy, reproducible, and guaranteed extractable petroleum hydrocarbon analysis.

Order yours today at www.restek.com/resprep

Resprep EPH Fractionation SPE Cartridges

C9

C20

C30

C40

Chlorooctadecane

Trimethylbenzene

Naphthalene

2-Bromonaphthalene

o-Terphenyl

Pyrene

Dibenz(a,h)anthracene

1 2 3 4 5 6 7 8 9 10 11 12 13Elution Volume (mL)

14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30


14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30


14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

Hexane /Aliphatic Fraction

Dichloromethane /Aromatic Fraction





00

0

Resprep EPH Fractionation SPE Cartridge, Lot 1



No guesswork, no worries.Detailed, step-by-step instructions and a complete certificate of analysis are included with every 15-pack of Resprep EPH cartridges.

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Figure 2: Storage stability is enhanced by new, moisture-resistant packaging that prevents ambient moisture from reaching your SPE cartridge and helps ensure outstanding performance.

Moisture Content after Storage in 80% Relative Humidity

0.000.501.001.502.002.503.003.504.004.505.00

0 1 2 3 4 6 8 10 12 16 20 24

Moi

stur

e (%

)

Storage Time (weeks)

Table I: Recovery data (n = 3) for Resprep EPH SPE cartridges. Again, 40+ compounds were analyzed; shown here are the compounds most critical to method requirements.

Compound Concentration (ppm) Function Average % Recovery %RSD

C9 200 Most-retained aliphatic 109.1 2.1%

Chlorooctadecane 200 Surrogate 107.0 0.6%

C28 4,000 Aliphatic loading capacity probe 109.2 1.0%

C40 200 Least-retained aliphatic 106.8 0.6%

Compound Concentration (ppm) Function Average % Recovery %RSD

Trimethylbenzene 200 Least-retained aromatic 85.3 6.7%

Naphthalene 200 Breakthrough probe 86.6 6.8%

2-Methylnaphthalene 200 Breakthrough probe 86.6 6.7%

2-Fluorobiphenyl 200 Surrogate 86.4 6.7%

2-Bromonaphthalene 200 Surrogate 86.2 6.7%

Phenanthrene 4,000 Aromatic loading capacity probe 86.2 6.7%

o-Terphenyl 200 Surrogate 86.2 6.7%

Dibenzo(a,h)anthracene 200 Most-retained aromatic 82.2 7.7%

Aliphatic Fraction

Aromatic Fraction

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Figure 3: Restek guarantees a background level in each fraction of less than half the strict reporting limits of MA and NJ EPH methods.

1-Chlorooctadecane at 5 ppm

Time (min)

5 7.5 10 12.5 15 17.5 20 22.5 25

Region 1Total extracted background = 8.2 ppm


EPH Silica Cartridge Background Extraction Aliphatic Fraction on Rxi-5ms

GC_EV1437

Conc. Peaks tR (min) (µg/mL)1. 1-Chlorooctadecane 15.679 5

Column Rxi-5ms, 30 m, 0.32 mm ID, 0.25 µm (cat.# 13424)Sample MA EPH surrogate spike mix (cat.# 31479)Conc.: 5 µg/mL InjectionInj. Vol.: 1 µL splitless (hold 1 min)Liner: Restek Premium 4.0 mm ID cyclo double taper inlet liner

(cat.# 23310)Inj. Temp.: 300 °CPurge Flow: 50 mL/minOven Oven Temp.: 40 °C (hold 1 min) to 300 °C at 12 °C/min to 330 °C at 6 °C/minCarrier Gas He, constant flowFlow Rate.: 2 mL/minLinear Velocity: 41.0 cm/sec @ 40 °CDead Time: 1.12 min @ 40 °CDetector FID @ 330 °CMake-up Gas Flow Rate: 45 mL/minMake-up Gas Type: N₂Hydrogen flow: 40 mL/minAir flow: 450 mL/minData Rate: 50 HzInstrument Agilent 7890B GCNotes The area of the surrogate peak was excluded from the

calculated total extracted background value for Region 2.

Conc. Peaks tR (min) (µg/mL)1. o-Terphenyl 14.177 5

Time (min)



o-Terphenyl at 5 ppm

5 7.5 10 12.5 15 17.5 20 22.5 25

EPH Silica Cartridge Background Extraction Aromatic Fraction on Rxi-5ms

GC_EV1438

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Description Volume qty. cat.#Resprep EPH Fractionation SPE Cartridge 25 mL, 5 g 15-pk. 25859

Resprep EPH Fractionation SPE Cartridge

• Method-specific performance for extractable petroleum hydrocarbon (EPH) analysis of soil and water samples.

• Complete separation of aliphatic and aromatic compounds into distinct fractions.• Guaranteed background level under the strict reporting limits of MA and

NJ EPH methods.• Superior lot-to-lot reproducibility and storage stability ensured by rigorous QC

testing and moisture-resistant packaging.

Product Listing

Resprep 12-Port SPE Manifold

Resprep 12- or 24-Port SPE Manifolds

• Use with any standard male luer end SPE cartridges.• Inert, PTFE sample guides reduce cross-contamination and carryover.• Flexible sample collection rack will accommodate a variety of receiving vessels.• Quick vacuum-release valve for better system control.• Individual valves allow vacuum control for each cartridge,

improving reproducibility.

Description qty. cat.#

Complete Resprep SPE Manifold, 12-Port (Includes: glass basin with built-in vacuum regulator (1); polypropylene lid with 12 individual control valves (1); 12-position collection rack (1); PTFE sample guides (12); and waste container (1).)

kit 26077

Complete Resprep SPE Manifold, 24-Port (Includes: glass basin with built-in vacuum regulator (1); polypropylene lid with 24 individual control valves (1); 24-position collection rack (1); and PTFE sample guides (24).)

kit 26080

Resprep® Manifold Replacement Parts

Description qty. cat.#

Replacement Waste Container, 12-Port ea. 24014 Replacement Vacuum Valve and Gauge Assembly ea. 24008 Glass Vacuum Chamber w/gauge & valve for Resprep manifolds, 6 or 12-Port ea. 25991

Collection Rack

Collection Rack, 12-Port ea. 26079 Collection Rack, 24-Port ea. 26082

Manifold Lid

Replacement Manifold Lid (sample guides not included), 12-Port ea. 26078 Replacement Manifold Lid (sample guides not included), 24-Port ea. 26081

Manifold Lid Replacement Parts

Valves, 12 or 24-Port 2-pk. 26083 Valve Attachment 48-pk. 26130 Needle Attachment 48-pk. 26131 Sample Guide Needles, 12 or 24-Port 12-pk. 26084

NOTE: Replacement waste container not included in 24-port manifold kit (cat.# 26080).*Waste container (shown in tank) and top shelf for round-bottom flasks are not included in 24-port manifold kit (cat.# 26080).

No-risk, no-obligation trial.Free samples of Resprep solid phase extrac-tion (SPE) tubes are available. Contact us or your local Restek representative to request yours: www.restek.com/contact-us

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100-pk. 1,000-pk.Description cat.# cat.#Amber Vial w/Graduated Marking Spot, 9 mm, Blue Cap PTFE/Silicone Liner 26596 26597

2.0 mL, 9 mm Screw-Thread Vial Convenience Kits (Vials, Caps, & Septa)

Vials packaged in a clear-lid tray. Caps with septa packaged in a plastic bag.

Cap Color Septa Material Type 100-pk. 1,000-pk.Blue PTFE/Silicone Screw-Thread 24485 24486

Choose preslit caps (available for some vials) to reduce the risk of needle bending, release vacuum from high-volume injections, and improve injection reproducibility when greater than 20% of vial volume is withdrawn.

2.0 mL, 9 mm Short-Cap, Screw-Vial Closures (Polypropylene, preassembled)

Description Color 100-pk. 1,000-pk.Short-Cap Vial w/White Graduated Marking Spot Amber 21142 21143

2.0 mL, 9 mm Short-Cap, Screw-Thread Vials (vial only)

2.0 mL, 9 mm Short-Cap, Screw-Thread Vials, 12 x 32 mmFit all 2.0 mL, 12 x 32 mm, crimp-top vial-based autosamplers.

Ideal for Agilent 7673, 7683, 7693 & other autosamplers that process

12 x 32 mm crimp-top vials.

Rxi-5ms Columns (fused silica)low-polarity phase; Crossbond diphenyl dimethyl polysiloxane

• General-purpose columns for semivolatiles, phenols, amines, residual solvents, drugs of abuse, pesticides, PCB congeners (e.g., Aroclor mixes), solvent impurities.

• Most inert column on the market.• Tested and guaranteed for ultra-low bleed;

improved signal-to-noise ratio for better sensitivity and mass spectral integrity.

• Temperature range: -60 °C to 330/350 °C.• Equivalent to USP G27 and G36 phases.

Description temp. limits qty. cat.#30 m, 0.32 mm ID, 0.25 µm -60 to 330/350 °C ea. 13424

similar phasesHP-5ms SemiVolatiles, HP-5ms, HP-5msUI, DB-5, Ultra-2, CP-Sil 8 CB, ZB-5, ZB-5msi

CH3

CH3

Si

95%

OSi

5%

O

Crimp-top and other vials also available! Find your vial at www.restek.com/vial-selector

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1,000 µg/mL each in methylene chloride, 1 mL/ampul cat.# 31458 (ea.)

Acenaphthene (83-32-9)Acenaphthylene (208-96-8)Anthracene (120-12-7)Benz(a)anthracene (56-55-3)Benzo(a)pyrene (50-32-8)Benzo(b)fluoranthene (205-99-2)Benzo(k)fluoranthene (207-08-9)Benzo(ghi)perylene (191-24-2)Chrysene (218-01-9)

Dibenz(a,h)anthracene (53-70-3)Fluoranthene (206-44-0)Fluorene (86-73-7)Indeno(1,2,3-cd)pyrene (193-39-5)2-Methylnaphthalene (91-57-6)Naphthalene (91-20-3)Phenanthrene (85-01-8)Pyrene (129-00-0)

MA EPH Aromatic Hydrocarbon Standard (17 components)

1,000 µg/mL each in hexane, 1 mL/ampul cat.# 31459 (ea.)

(C9) n-Nonane (111-84-2)(C10) n-Decane (124-18-5)(C12) n-Dodecane (112-40-3)(C14) n-Tetradecane (629-59-4)(C16) n-Hexadecane (544-76-3)(C18) n-Octadecane (593-45-3)(C19) n-Nonadecane (629-92-5)

(C20) n-Eicosane (112-95-8)(C22) n-Docosane (629-97-0)(C24) n-Tetracosane (646-31-1)(C26) n-Hexacosane (630-01-3)(C28) n-Octacosane (630-02-4)(C30) n-Triacontane (638-68-6)(C36) n-Hexatriacontane (630-06-8)

MA EPH Aliphatic Hydrocarbon Standard (14 components)

2,000 µg/mL each in hexane:carbon disulfide (80:20), 1 mL/ampul cat.# 30540 (ea.)

(C9) n-Nonane (111-84-2)(C10) n-Decane (124-18-5)(C12) n-Dodecane (112-40-3)(C14) n-Tetradecane (629-59-4)(C16) n-Hexadecane (544-76-3)(C18) n-Octadecane (593-45-3)(C20) n-Eicosane (112-95-8)(C21) n-Heneicosane (629-94-7)(C22) n-Docosane (629-97-0)(C24) n-Tetracosane (646-31-1)

(C26) n-Hexacosane (630-01-3)(C28) n-Octacosane (630-02-4)(C30) n-Triacontane (638-68-6)(C32) n-Dotriacontane (544-85-4)(C34) n-Tetratriacontane (14167-59-0)(C36) n-Hexatriacontane (630-06-8)(C38) n-Octatriacontane (7194-85-6)(C40) n-Tetracontane (4181-95-7)2-Methylnaphthalene (91-57-6)Naphthalene (91-20-3)

NJDEP EPH 10/08 Rev. 2 Aliphatics Calibration Standard (20 components)

2,000 µg/mL each in methylene chloride, 1 mL/ampul cat.# 30541 (ea.)

Acenaphthene (83-32-9)Acenaphthylene (208-96-8)Anthracene (120-12-7)Benz(a)anthracene (56-55-3)Benzo(a)pyrene (50-32-8)Benzo(b)fluoranthene (205-99-2)Benzo(ghi)perylene (191-24-2)Benzo(k)fluoranthene (207-08-9)Chrysene (218-01-9)

Dibenz(a,h)anthracene (53-70-3)Fluoranthene (206-44-0)Fluorene (86-73-7)Indeno(1,2,3-cd)pyrene (193-39-5)2-Methylnaphthalene (91-57-6)Naphthalene (91-20-3)Phenanthrene (85-01-8)Pyrene (129-00-0)1,2,3-Trimethylbenzene (526-73-8)

NJDEP EPH 10/08 Rev. 2 Aromatics Calibration Standard (18 components)

200 µg/mL each in pentane, 5 mL/ampul cat.# 30542 (ea.)

(C9) n-Nonane (111-84-2)(C10) n-Decane (124-18-5)(C12) n-Dodecane (112-40-3)(C14) n-Tetradecane (629-59-4)(C16) n-Hexadecane (544-76-3)(C18) n-Octadecane (593-45-3)(C20) n-Eicosane (112-95-8)(C21) n-Heneicosane (629-94-7)(C22) n-Docosane (629-97-0)

(C24) n-Tetracosane (646-31-1)(C26) n-Hexacosane (630-01-3)(C28) n-Octacosane (630-02-4)(C30) n-Triacontane (638-68-6)(C32) n-Dotriacontane (544-85-4)(C34) n-Tetratriacontane (14167-59-0)(C36) n-Hexatriacontane (630-06-8)(C38) n-Octatriacontane (7194-85-6)(C40) n-Tetracontane (4181-95-7)

NJDEP EPH 10/08 Rev. 2 Aliphatics Matrix Spike Mix (18 components)

200 µg/mL each in acetone:toluene (50:50), 5 mL/ampul cat.# 30543 (ea.)

Acenaphthene (83-32-9)Acenaphthylene (208-96-8)Anthracene (120-12-7)Benz(a)anthracene (56-55-3)Benzo(a)pyrene (50-32-8)Benzo(b)fluoranthene (205-99-2)Benzo(ghi)perylene (191-24-2)Benzo(k)fluoranthene (207-08-9)Chrysene (218-01-9)

Dibenz(a,h)anthracene (53-70-3)Fluoranthene (206-44-0)Fluorene (86-73-7)Indeno(1,2,3-cd)pyrene (193-39-5)2-Methylnaphthalene (91-57-6)Naphthalene (91-20-3)Phenanthrene (85-01-8)Pyrene (129-00-0)1,2,3-Trimethylbenzene (526-73-8)

NJDEP EPH 10/08 Rev. 2 Aromatics Matrix Spike Mix (18 components)

400 µg/mL each in hexane, 5 mL/ampul cat.# 30544 (ea.)

(C9) n-Nonane (111-84-2)(C10) n-Decane (124-18-5)(C12) n-Dodecane (112-40-3)(C14) n-Tetradecane (629-59-4)(C16) n-Hexadecane (544-76-3)(C18) n-Octadecane (593-45-3)(C20) n-Eicosane (112-95-8)(C21) n-Heneicosane (629-94-7)(C22) n-Docosane (629-97-0)

(C24) n-Tetracosane (646-31-1)(C26) n-Hexacosane (630-01-3)(C28) n-Octacosane (630-02-4)(C30) n-Triacontane (638-68-6)(C32) n-Dotriacontane (544-85-4)(C34) n-Tetratriacontane (14167-59-0)(C36) n-Hexatriacontane (630-06-8)(C38) n-Octatriacontane (7194-85-6)(C40) n-Tetracontane (4181-95-7)

NJDEP EPH 10/08 Rev. 2 Aliphatics Fractionation Check Mix (18 components)

400 µg/mL each in hexane:toluene (50:50), 5 mL/ampul cat.# 30545 (ea.)

Acenaphthene (83-32-9)Acenaphthylene (208-96-8)Anthracene (120-12-7)Benz(a)anthracene (56-55-3)Benzo(a)pyrene (50-32-8)Benzo(b)fluoranthene (205-99-2)Benzo(ghi)perylene (191-24-2)Benzo(k)fluoranthene (207-08-9)

Chrysene (218-01-9)Dibenz(a,h)anthracene (53-70-3)Fluoranthene (206-44-0)Fluorene (86-73-7)Indeno(1,2,3-cd)pyrene (193-39-5)Phenanthrene (85-01-8)Pyrene (129-00-0)1,2,3-Trimethylbenzene (526-73-8)

NJDEP EPH 10/08 Rev. 2 Aromatics Fractionation Check Mix (16 components)

4,000 µg/mL each in acetone, 1 mL/ampul cat.# 31479 (ea.)

1-Chlorooctadecane (3386-33-2)o-Terphenyl (84-15-1)

MA EPH Surrogate Spike Mix (2 components)

Learn more about Restek reference standards at www.restek.com/standards

Questions about this or any other Restek product? Contact us or your local Restek representative (www.restek.com/contact-us).Restek patents and trademarks are the property of Restek Corporation. (See www.restek.com/Patents-Trademarks for full list.) Other trademarks in Restek literature or on its website are the property of their respective owners. Restek registered trademarks are registered in the U.S. and may also be registered in other countries.

© 2016 Restek Corporation. All rights reserved. Printed in the U.S.A.

www.restek.com Lit. Cat.# EVSS2574-UNV

Proven Quality • Superior Cleanliness • Method-Specific Performance

Solid Phase Extraction Cartridges

Available with the following sorbents:

• Silica: Multipurpose

• EPH Silica: Petroleum

• Florisil Adsorbent: Pesticides

• CarboPrep Adsorbent: Dirty Samples

FREE samples available! Contact us or your local Restek representative.

Learn more at www.restek.com/resprep

Method-Specific Sample Preparation for EPH Analysis · Method-Specific Sample Preparation for EPH Analysis Resprep SPE Cartridges for Complete Extractable Petroleum Hydrocarbon Fractionation

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