Research Center for Civilization Diseases
LIFE – Research Center for Civilization DiseasesLeipzig
University Philipp-Rosenthal-Str. 2704103
[email protected]
contact person: Prof. Dr. Uta Ceglarek
[email protected]
Mass SpectrometryProteomics and Metabolomics
In the LIFE Research Center
LIFE is funded by means of the European Union, by the European
Regional Development (ERDF) an by means of the Free State of Saxony
withhin the framework of the excellence initiative.
Institute of Laboratory Medicine, Clinical Chemistry and
Molecular Diagnostics University Hospital Leipzig AöR Liebigstr.
2704103 Leipzig
director: Prof. Dr. Joachim Thiery
www.ilm.uniklinikum-leipzig.de
• Preanalytical Investigation of Polyunsaturated Fatty Acids and
Eicosanoids in Human Plasma by Liquid Chromatography-Tandem Mass
Spectrometry; Dorow et al.; Biopreserv Biobank; 2016; 14(2);
107-113.
• Immunoassay or LC-MS/MS for the measurement of salivary
cortisol in children? Bae, Gaudl et al.; Clin Chem Lab Med; 2016;
54(5); 811-822.
• LC-MS/MS-based quantification of cholesterol and related
metabolites in dried blood for the screening of inborn errorsof
sterol metabolism; Becker et al.; Anal Bioanal Chem; 2015; 407(17);
5227-5233.
• Preanalytical standardization of sphingosine-1-phosphate,
sphinganine-1-phosphate and sphingosine ana-lysis in humanplasma by
liquid chromatography-tandem mass spectrometry; Ceglarek et al.;
Clin Chim Acta; 2014; 435; 1-6.
• Preanalytical standardization for reactive oxygen species
derived oxysterol analysis in human plasma by liquid
chromatography-tandem mass spectrometry; Helmschrodt et al.;
Biochem Biophys Res Commun; 2014; 446(3); 726-730.
• Quantification of seven apolipoproteins in human plasma by
proteotypic peptides using fast LC-MS/MS;Ceglarek, Dittrich et al.;
Proteomics Clin Appl; 2013; 11-12; 794-801.
• Fast LC-MS/MS analysis of free oxysterols derived from
reactive oxygen species in human plasma and carotid
plaque;Helmschrodt et al.; Clin Chim Acta; 2013; 425; 3-8.
• Fast liquid chromatography-quadrupole linear ion trap-mass
spectrometry analysis of polyunsaturated fatty acids andeicosanoids
in human plasma; Kortz et al.; J Chromatogr B Analyt Technol Biomed
Life Sci; 2013; 927; 209-13.
Services Metabolomics and proteomics in the LIFE research
program
The coupling of liquid chromatography and mass spectrometry
enables rapid and highly sensitive analysis of biologically active
metabolites, peptides, and proteins in body fluids. Within the LIFE
research program a mass spectrometric platform for metabolom and
proteom analysis has been established, allowing targeted and
simultaneous quantitation of metabolites and proteins. By means of
ultra-high per-formance liquid chromatography (UHPLC) in
combination with quadrupol linear ion trap mass spectrometry, it is
possible to detect and quantitate metabolites in con-centration
ranges of ng/L.
In the past years method development focused on metabolic
pathways that are related to vascular and cardiovascular di-seases
such as arteriosclerosis or cardiac insufficiency, as well as
diabetes and de-mentia. Standardized preanalytical proto-cols
covering sample drawing, handling, and sample preparation were
developed for ensuring reliable quantitation of partly very low
abundant metabolites and pro-teins in body fluids. Methods
developed in the mass spectrometry workgroup were validated for the
analysis in body fluids and were partly accredited according DIN EN
ISO 15189:2014 and 17025:2005.
Standardized Preanalytical Protocols
State of the Art mass Spectrometry
Quality ensured Quantitative Analysis according FDA and CLSI
Standardized Data Processing
In the LIFE research program, samples from patient popula-tions
are used to create biomarker profiles paving the way for
identifying novel candidate genes for civilization diseases in the
line of genome-wide association studies (GWAS). Aim of method
development is their potential future application in the clinical
routine laboratory. Since the beginning of the year 2016, the
simultaneous quantitation of nine steroid hormo-nes via liquid
chromatography tandem mass spectrometry is pioneeringly and
invariably applied to routine diagnostic ana-lysis at the Institute
of Laboratory Medicine, Clinical Chemistry and Molecular
Diagnostics of University Hospital Leipzig AöR.
Analyte classesSteroid hormones*Apolipoproteins*Amino
Acids/AcylcarnitinesSterol Lipids*Cholesterol
EstersEicosanoids/ResolvinsPoly-Unsaturated Fatty Acids
Sphingolipids*OxysterolsBile
AcidsPhospholipidesEndocannabinoidsTriglycerides
*accredited according DIN EN ISO 15189:2014 and DIN EN ISO
17025:2005