4125 Beaumont Road | Lansing, MI 48910 | www.animalhealth.msu.edu Laboratory Diagnosis of Coronavirus Infections in Ferrets Laboratory Diagnosis of Coronavirus Infections in Ferrets Roger K. Maes, Annabel G. Wise, Michael M. Garner, Matti Kiupel Roger K. Maes, Annabel G. Wise, Michael M. Garner, Matti Kiupel
21
Embed
Laboratory Diagnosis of Coronavirus Infections in Ferrets Laboratory
This document is posted to help you gain knowledge. Please leave a comment to let me know what you think about it! Share it to your friends and learn new things together.
Transcript
4125 Beaumont Road | Lansing, MI 48910 | www.animalhealth.msu.edu
Laboratory Diagnosis of Coronavirus Infections in Ferrets
Laboratory Diagnosis of Coronavirus Infections in Ferrets
Roger K. Maes, Annabel G. Wise, Michael M. Garner, Matti Kiupel
Roger K. Maes, Annabel G. Wise, Michael M. Garner, Matti Kiupel
4125 Beaumont Road | Lansing, MI 48910 | www.animalhealth.msu.edu
Coronavirus CharacteristicsCoronavirus Characteristics• Glycoproteins of envelope:
S – SpikeM – MembraneE – Envelope
• Genome: + sense RNAnon-segmentedsingle-stranded27-31 kb
• RNA enclosed in capsid of N protein monomers
• Glycoproteins of envelope:S – SpikeM – MembraneE – Envelope
• Genome: + sense RNAnon-segmentedsingle-stranded27-31 kb
• RNA enclosed in capsid of N protein monomers
4125 Beaumont Road | Lansing, MI 48910 | www.animalhealth.msu.edu
Coronavirus PhylogenyCoronavirus Phylogeny
Woo et al. Exp. Biol. and Medicine (2009)Woo et al. Exp. Biol. and Medicine (2009)
4125 Beaumont Road | Lansing, MI 48910 | www.animalhealth.msu.edu
• Novel group 1 coronavirus associated with epizootic catarrhal enteritis (ECE) in ferrets (Wise et al., 2006 Virology 349: 164-174)
• Novel group 1 coronavirus associated with epizootic catarrhal enteritis (ECE) in ferrets (Wise et al., 2006 Virology 349: 164-174)
4125 Beaumont Road | Lansing, MI 48910 | www.animalhealth.msu.edu
Ferret Systemic Coronavirus(FRSCV)
Ferret Systemic Coronavirus(FRSCV)
• FIP-like disease observed in ferrets in Europe and the U.S. (2002-2007)
• Common clinical findings: anorexia, weight loss, lethargy, CNS signs, palpable intra-abdominal mass or masses
• FIP-like lesions: systemic pyogranulomatousinflammation in several abdominal and thoracic organs
• FIP-like disease observed in ferrets in Europe and the U.S. (2002-2007)
• Common clinical findings: anorexia, weight loss, lethargy, CNS signs, palpable intra-abdominal mass or masses
• FIP-like lesions: systemic pyogranulomatousinflammation in several abdominal and thoracic organs
4125 Beaumont Road | Lansing, MI 48910 | www.animalhealth.msu.edu
FIP-like Disease in FerretsFIP-like Disease in Ferrets
Widespread nodular foci on serosalsurfaces of organs.
Widespread nodular foci on serosalsurfaces of organs.
4125 Beaumont Road | Lansing, MI 48910 | www.animalhealth.msu.edu
Ferret Systemic Coronavirus(FRSCV)
Ferret Systemic Coronavirus(FRSCV)
Comparative sequence analysis of the distal one-third of the genomes of a systemic and an enteric ferret coronavirus ( Wise et al. Virus Research 149 (2010) 42-50
4125 Beaumont Road | Lansing, MI 48910 | www.animalhealth.msu.edu