Lab 11& 12 Lab 11& 12 Biochemical Biochemical Characterization Characterization Practice questions Designed by Pyeongsug Kim ©2010 [email protected] Fall 2011 Chattahoochee Technical College Picture from http://www.midlandstech.edu/science/kelleherk/225/labmaterials/sel_diff_media.html
Lab 11& 12 Biochemical Characterization Practice questions Designed by Pyeongsug Kim ©2010 [email protected]@science-i.com Fall 2011 Chattahoochee.
Dec 16, 2015
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Lab 11& 12Lab 11& 12Biochemical CharacterizationBiochemical Characterization
Practice questions
Designed by Pyeongsug Kim ©2010 [email protected]
Fall 2011Chattahoochee Technical College
Picture from http://www.midlandstech.edu/science/kelleherk/225/labmaterials/sel_diff_media.html
Designed by Pyeongsug Kim, ©2010 www.science-i.com
What is the name of each medium? Identify whether each agar is selective or differential.
Picture from http://www.cfkeep.org/html/stitch.php?s=72880656991974&id=24286258057026
D MSA agar – both selective and differential
TSA agar – Neither
Blood agar – Differential
EMB agar – both selective and differential
A:B:C:D:
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What is the name of the media above?
Is selective or differential media? Explain your answer.
Is it shown positive result of negative result in each medium?A:B:C:
Picture from http://archive.microbelibrary.org/ASMOnly/details.asp?id=2553&Lang
A B C
EMB agar
Both selective and differentialSelective ~inhibit the growth of Gram positive bacteria (selective for Gram negative)Differential ~ differentiates coliforms (Lactose fermenters)
Positive (lactose fermentation, coliforms)Positive (Lactose fermentation, coliforms)Negative
What is the name of the media above?
Is selective or differential media? Explain your answer.
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A A
Is it shown positive result of negative result in each medium?A:B:
What is the name of the media above?
Is selective or differential media? Explain your answer.
MSA plate
Both selective and differentialSelective ~Selective for Gram positive and Salt tolerantDifferential ~ differentiates for mannitol fermentation
Positive (manitol fermentation)Negative
Picture from http://lifesci.rutgers.edu/skelly/images/Msa%20Plates.htm
What results are shown?A:B:C:
Picture from http://iws2.collin.edu/dcain/CCCCD%20Micro/hemolysis.htm
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What is the name of the test above?What is the name of media above?
Is selective or differential media? Explain your answer.
Hemolysys test
Differential ~ differentiates for hemolysis
Alpha hemolysisGamma hemolysisBeta hemolysis
Blood agar
A B CB
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What properties of bacterial metabolism are demonstrated above?A:B:C:
Is selective or differential media? Explain your answer.
Sugar fermentation
Differential ~ differentiates for sugar fermentation
Positive ~ Lactose fermentation (acid/ No gas)Positive ~ Dextrose fermentation (acid/gas forming)Negative for maltose fermentation (No acid/ No gas)
Phenol red broth with sugar
Each tube contains ….A: LactoseB: DextroseC: Maltose.
A B C
Picture from http://a-s.clayton.edu/furlong/BIOL3250/lab/Review/page/biochemicaltestanswer.htm
What is the name of media?
Semisolid media
What properties of bacterial metabolism are demonstrated above?A:B:
A BPositive for Hydrogen SSulfide productionNegative for Hydrogen SSulfide production
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What is the name of media? Is it selective or differential? SIM -Differential
A BWhat name of the media is shown? Is it selective or differential? SIM; Differential
What properties of bacteria is shown?A: B:
MotilityNonmotileMotile
Picture from http://inst.bact.wisc.edu/inst/index.php?module=book&func=displayarticle&art_id=118
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Kovac’s reagent
What is the name of media? Is it selective or differential? SIM; Differential
What name of the test is? Indole test
A BControl
What biological prosperity do this bacteria have?A:B:
Negative for indole productionPositive for indole production
Picture from http://subota.svetlostistine.org/negative-indole-test&page=6
What is the name of media? Is it selective or differential?
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Methyl red
Picture from http://www.mesacc.edu/~johnson/labtools/Dbiochem/imvic.html
What is the name of the test(left)?MR-VP; Differential
What biological prosperity do this bacteria have?A:B:
A B
Negative for Mixed acid productionPositive for Mixed acid production
VP testWhat is the name of the test(left)?
What biological prosperity do this bacteria have?A:B:
MR test
Negative for acetoin productionPositive for acetoin production
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AB
Before putting the reagent.
After putting the reagent.
What is the name of the test(left) using a starch agar?
What biological prosperity do this bacteria have?A:B:
Extracellular amylase test
Negative-No amylase presencePositive - Amylase presence
What is the name of the reagent that used in this test?
Gram-Iodine
Which one, A or B, is shown starch presence? A
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What is the name of the test(left)?
Hydrogen peroxide
Picture from http://iws2.collin.edu/dcain/CCCCD%20Micro/catalase_test.htm
Catalase test
What type of reagent is using for this test?
What biological prosperity do this bacteria have?A:B:
Negative – Catalase presence.Positive – Catalase presence
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