Kilimanjaro Clinical Research Institute (KCRI)

Davis Kuchaka BSc., MSc.

Monday, July 8, 2019

Kilimanjaro Clinical Research Institute (KCRI)

Outline• AFB microscopy

• Smear preparation

• The cell wall

• Staining

• Reporting

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AFB Microscopy

2

Acid fast Bacilli – resist decolorization

with acid alcohol

Microscopes

• Bright field

• Fluorescent

Smear preparation• Direct smear

– Sputum

• Concentrated

– Decontaminated sputum

• Culture

– MGIT

– Solid

3

The Cell wall

4

Staining

Auramine

Ziehl-Neelsen

5

ZN stained smear

6

Auramine stained smear

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Reporting

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Reporting

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Which piece of the puzzle?

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Resources used • https://www.chemonics.com/wp-

content/uploads/2016/03/2016_03.24_Kankadze_WorldTBDay_2.jpg

• NC008 Mycobacteriology Lab Manual

• http://stoptb.org/wg/gli/assets/documents/TB%20MICROSCOPY%20HANDBOOK_FINAL.pdf

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