Kidney function test. Functions of the kidney Regulation e.g. homeostasis,water, acid/base Excretion e.g.uric acid, urea, creatinine Endocrine e.g. renin,

Kidney function test

Functions of the kidney

Regulation e.g. homeostasis ,water, acid/base

Excretion e.g.uric acid, urea, creatinine

Endocrine e.g. renin, erythropoietin, 1,25 dihydroxycholecalciferol- conversion only in kidney!

When should you assess renalfunction?

Risk factors for kidney disease: – Older age – Family history of Chronic Kidney disease (CKD) – Decreased renal mass – Low birth weight – US racial or ethic minority – Low income – Lower education level – Diabetes Mellitus (DM) – Hypertension (HTN) – Autoimmune disease – Systemic infections – Urinary tract infections (UTI) – Nephrolithiasis – Obstruction to the lower urinary tract – Drug toxicity

Uric acid

Uric acid is a chemical created when the body breaks down substances called purines.

Purines are found in some foods and drinks, such as liver, anchovies, mackerel, dried beans and peas, beer, and wine.

Purines are also a part of normal body substances, such as DNA.

In humans, approximately 75% of uric acid excreted is lost in the urine; most of the reminder is secreted into the GIT

If your body produces too much uric acid or doesn't remove enough if it, you can get sick.

High levels of uric acid in the body is called hyperuricemia

Normal values fall between 3.0 and 7.0 mg/dL. Note: Normal values may vary slightly from

laboratory to laboratory.

When is it ordered?

when a doctor suspects high levels of uric acid :

Gout monitoring test when a patient has undergone chemotherapy or

radiation

if a patient appears to have failing kidneys

Greater-than-normal levels of uric acid (hyperuricemia) may be due to:

Alcoholism Diabetes Gout Hypoparathyroidism Lead poisoning Leukemia Nephrolithiasis Polycythemia vera Renal failure Toxemia of pregnancy Purine-rich diet Excessive exercise Chemotherapy-related side effects

Lower-than-normal levels of uric acid may be due to:

Fanconi syndrome Wilson's disease Syndrome of inappropriate antidiuretic hormone (SIADH)

secretion Multiple Sclerosis Low purine die

Gout Gout is a kind of arthritis that occurs when uric acid builds up in

the joints. In Gout increased serum levels of uric acid lead to formation of

monosodium urate crystals around the joints. Acute gout is a painful condition that typically affects one joint. Chronic gout is repeated episodes of pain and inflammation,

which may involve more than one joint. The exact cause is unknown. Gout may run in families. It is more

common in males, postmenopausal women, and people who drink alcohol

Symptoms of acute gouty attacks:

Symptoms develop suddenly and usually involve only one or a few joints. The big toe, knee, or ankle joints are most often affected.

The pain frequently starts during the night and is often described as throbbing, crushing, or excruciating.

The joint appears warm and red. It is usually very tender.

There may be a fever. The attack may go away in several days, but may

return from time to time. Additional attacks usually last longer.

After a first gouty attack, people will have no symptoms. Some people will go months or even years between gouty attacks.

Some people may develop chronic gouty arthritis, but others may have no further attacks. Those with chronic arthritis develop joint deformities and loss of motion in the joints. They will have joint pain and other symptoms most of the time.

Tophi are lumps below the skin around joints or in other places. They may drain chalky material. Tophi usually develop only after a patient has had the disease for many years.

After one gouty attack, more than half of people will have another attack.

A tophus on the elbow of a middle aged man with chronic gout.

Tests that may be done include:

Synovial fluid analysis

Gold standard to confirm gout,

Urate crystals identified by:

- Needle and rod shapes

Uric acid - blood Joint x-rays )may be normal)

X-ray of gouty uric acid deposit in the big toe (left)

Possible Complications

Chronic gouty arthritis Kidney stones Deposits in the kidneys, leading to chronic kidney

failure

Treatment

Treatments for a sudden attack or flare-up of gout: Your doctor will recommend that you take

nonsteroidal anti-inflammatory drugs (NSAIDs) such as ibuprofen, naproxen, or indomethacin as soon as your symptoms begin

Your health care provider may occasionally prescribe strong painkillers such as codeine

Daily use of allopurinol decrease uric acid levels in your blood

Some diet and lifestyle changes may help

prevent gouty attacks:

Avoid alcohol, sardines, oils, organ meat (liver, kidney, and sweetbreads(

Limit how much meat you eat at each meal. Avoid fatty foods such as salad dressings, ice

cream, and fried foods Eat enough carbohydrates If you are losing weight, lose it slowly. Quick weight

loss may cause uric acid kidney stones to form.

Note:

Uric acid may also form kidney stones

Although uric acid can act as an antioxidant, excess serum accumulation is often associated with cardiovascular disease.

urea Urea is a relatively nontoxic nitrogen containing substance made by

the liver to dispose of ammonia resulting from protein metabolism.

Its elimination in the urine represents the major route for nitrogen excretion.

The reference interval for serum urea of healthy adults is 5-39 mg/dl (slightly higher in males than females)

BUN = (blood urea nitrogen)

The real urea concentration is BUN x 2.14

Increased BUN = Azotemia

Causes:– Increased protein catabolism: Increased dietary protein Severe tress: MI, fever, etc Rhabdomyolysis Upper GI bleeding– Impaired renal function Pre renal azotemia: renal hypoperfusion Renal azotemia: acute tubular necrosis Post renal azotemia: obstruction of urinary flow

Decreased BUN

Decreased dietary protein Increased protein synthesis ( Pregnant women , children ) severe liver disease SIADH Overhydration (IV fluids)

The key to identifying the azotemia as prerenal is the increase of plasma urea without parallel increase of plasma creatinine.

With obstruction, both plasma urea and creatinine increase, but there is greater rise of urea than of creatinine because the obstruction of urine flow backpressure on the tubule and back diffusion of urea into blood from the tubule.

BUN / Creatinine RatioBUN / Creatinine Ratio

Pre-renal BUN is more susceptible

to non-renal factors

Post-renalPost-renal Both BUN and Creat. are

elevated

Increased BUN

Normal Creat

Increased BUN

Increased Creat

creatinine

Creatine is synthesized in the kidneys and liver

It is then transported in blood to other organs such as muscle and brain, where it is phosphorylated to phosphocreatine, a high-energy compound.

Interconversion of phosphocreatine and creatine is a particular feature of metabolic processes of muscle contraction

1 to 2% of muscle creatine spontaneously converts to creatinine daily and released into body fluids at a constant rate.

Endogenous creatinine produced is proportional

to muscle mass, it is a function of total muscle mass the production varies with age and sex

creatinine

The breakdown product of creatine phosphate released from skeletal muscle at a steady rate.

It is filtered by the glomerulus. It is generally a more sensitive and specific test

for renal function than the BUN. Normal range is 0.6-1.3 mg/dL– *non pregnant

state-*

Increased serum creatinine:

– Impaired renal function – Very high protein diet – Vary large muscle mass: body builders, giants, acromegaly

patients – Rhabdomyolysis/crush injury – Athletes taking oral creatine – Drugs:

• Probenecid• Cimetidine

• Triamterene• Trimethoprim

• Amiloride

Principle of of Clearance

Some substances when filtered enter the tubules are not reabsorbed and so 100% excreted= GFR (inulin= gold standard for GFR, creatinine (but this one partially reabsorbed, particularly in uremia, then clearance <GFR

Some substances are filtered, enter tubules, and more of the substance is secreted enters the tubules by excretion. Clearance>GFR

Some substances are filtered, enter tubules, but are completely reabsorbed, so they did not reach the final urine (e.g. cystatin C)

Clearance is defined as the (hypothetical) quantity of blood or plasma completely cleared of a substance per unit of time.

The most frequently used clearance test is based on the measurement of creatinine.

The 'clearance' of creatinine from plasma is directly related to the GFR if:

The urine volume is collected accurately There are no ketones or heavy proteinuria present to

interfere with the creatinine determination.

It should be noted that the GFR decline with age (to a greater extent in males than in females) and this must be taken into account when interpreting results.

Creatinine clearance in adults is normally about of 120 ml/min

Plasma creatininePlasma creatinine

Plasma creatinine concentration is inversely related to the GFR But GFR can decrease by 50% before plasma creatinine

concentration rises beyond the normal range this means that a normal plasma creatinine does not necessarily imply normal renal function,

A Raised creatinine usually indicates impaired renal function Changes in plasma creatinine concentration can occur,

independently of renal function, due to changes in muscle mass.

Decrease can occur as a result of starvation and in wasting diseases, immediately after surgery.