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Identification of Wuchereria bancrofti in Culex quinquefasciatus
Post-Mass Drug Administration
(MDA) Lymphatic Filariasis in Bekasi District, Indonesia
Endang Puji Astuti*, Joni Hendri*, Mara Ipa*, Andri Ruliansyah*,
Triwibowo Ambar Garjito, Mega Tyas Prihatin
1
*)Pangandaran Unit for Health Research and Development, National
Institute of Health Research and Development, Ministry of Health of
Indonesia, Pangandaran,
West Java, Indonesia
*e-mail: [email protected]
mailto:[email protected]
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Introduction
Methods
Results and Discussion
Conclusions
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Outline
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Lymphatic filariasis (LF) as a neglected tropical disease
(NTD)
World Health Assembly Resolution 50.29 (May, 1997) - elimination
of LF as a public health problem by 2020
LF affected 893 million people in 49 countries around the
world
Introduction
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LF elimination program in Indonesia, until 2019 there were 236
out of 514 districts endemic areas, yet only 118 districts had
implemented MDA-LF
Bekasi district has performed post MDA assessmenet (TAS II) to
evaluate the efficacy of the MDA program.
It’s revealed positive circulating filarial antigen (CFA) in
human blood, indicating a newly emerged LF vector.
The detection of filarial DNA in mosquitoes to provide an
indirect indicator of infected community
Recent microfilaria (Mf) status in mosquitoes is unclear.
Introduction
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This study was to determine presence of W. bancrofti in
Culexquinquefasciatus as suspected vector in Bekasi district
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Objectives
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Study Site
Methods
Mosquitoes sampling and PCR analysis
Data analysis6
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Study Site
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The study was conducted in October 2019 at selected LF endemic
village in Jatimulya, Tambun Subdistrict, Bekasi District
Indonesia
A total of 13 out of 23 sub-district in Bekasi District were
reported the chronic LF patients from 2000 – 2019
A high number of LF chronic cases was identified in Tambun
sub-district which Jatimulya as sentinel spot site of LF
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• Mosquitoes were collected in two locations at sentinel and
spot site
• The study was conducted during period temperatures ranged
between 28.7 – 31.3°C with a relative humidity of 60 - 63%.
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Mosquitoes sampling PCR and Data analysis
Reference :Inner bednet protecting sleeping bite (Charlwood et
al, 1968)
Human –Baited double net trap with collecting cup (Julie-Anne et
al, 2015; Service MW, 1977)
HDNs located outside of catching station
HDNs located inside of catching station
Analysis of Man Bitting Rate (MBR), Man Hour density (MHD) based
on WHO (1975)
measure of daily survival value (longevity) and estimated
population age of mosquitoes based on Davidson (Gilles and
Warrel)
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Results and Discussion
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10
77,12
22,88
nulliparous parous
Parity rate of Cx. quinquefasciatus in Jatimulya, Bekasi at
October 2019
Man Bitting Rate Cx. quinquefaciatus using HDN indoor and
outdoor in Bekasi, 2019
Bionomic of LF Vector
N = 673
N = 154
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Man Hour Density Cx. quinquefasciatus using HDN indoor and
outdoor in Bekasi, 2019
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Identification of W. bancrofti in Cx. quinquefasciatus (48
pooling) in Bekasi, 2019
Parameter Index
Parous 154
Parous proportion 0.2288262
Gonotrophic cycle ‡ (day) 4
Daily survival value (p) 0.6916341
(-) Ln B -0.368698
Estimated population age (day) 2.703
‡ Julian E. García-Rejón, Jose A. Farfan-Al et al. (2008)
Estimated age population of Cx. quinquefasciatus in Bekasi,
October 2019
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This study has shown the nonexistence of microfilaria W.
bancrofti in LF vector Cx. quinquefasciatus, which may indicated a
low-level of LF transmission in Jatimulya village, Bekasi.
Our findings suggested, routine vector surveillance for
monitoring remain essential to be done to ensure the sustainability
of LF elimination program and to control the re-emergence of LF
cases in the area studied
Conclusion
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References
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pntd.00
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Meet
Our
Team
Endang Puji Astuti Mara IpaJoni Hendri
Andri Ruliansyah Triwibowo A. G. Mega Tyas P.
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Thank You