Page 1 human Platelet Lysate (hPL) Intended for research use only. Not to be used in diagnostic or therapeutic procedures. 250 ml 820708-hpl RPMI 1640 medium kit, together with hPL supplement (final conc. 5%) 250 ml 820408-hpl DMEM:Ham's F12 medium kit, together with hPL supplement (final conc. 5%) Related products CLS Product information: human Platelet Lysate (hPL) hPL Product name: 860065 Cat. no.: 12.5 ml Size: Handling of the supplement: Remove the supplement hPL from -70°C and thaw in a prewarmed 37°C water bath. Aseptically add the complete contents of the vial to the appropriate medium using a 10 ml serological pipette. Thoroughly mix the fluids without producing foam. Quality control: The hPL batches are tested for sterility, for the absence of Mycoplasma contaminations and for bacterial endotoxins. Tested negative for HIV,HBV, HCV and more. A certificate for each lot is available upon request. Storage recommendations: Store the hPL supplement at -70°C. The product is stable to the date as indicated on the label when stored properly. Once the medium was supplemented with hPL, the maximum shelf life is 14 days. Please note the date of the supplementation on the label of the medium bottle. Always store the complete medium refrigerated if it is not in use. Applications: - Replacement of FBS in cell culture media. - Animal-component-free culture of hMSCs (human Mesenchymal Stromal cells). - Animal-component-free culture of human stem cells. - Animal-component-free culture of continuous cell lines. Product description: The supplement hPL (human Platelet lysate) is manufactured from platelet single donor apheresis samples which are collected at blood banks in Southern Germany. Each donor is tested twice, at the time of donation and 4 months later. Only those platelets from double-negative tested donors are used to manufacture the platelet lysates. Each lysate represents a pool from 25 donors. 250 ml 820108-hpl EMEM medium kit, together with hPL supplement (final conc. 5%) 250 ml 820308-hpl DMEM medium kit, together with hPL supplement (final conc. 5%) 250 ml 820608-hpl Ham's F12 medium kit, together with hPL supplement (final conc. 5%) From one up to ten units with 12.5 ml each; staggered pricing. Units: 250 ml 820808-hpl IMDM medium kit, together with hPL supplement (final conc. 5%) 100 ml 820708-hpl Accutase, cell detachment solution, sterile, ready-to-use 25 ml 800625 CM-ACF, freeze medium, serum free, ready-to-use 50 ml 800650 CM-ACF, freeze medium, serum free, ready-to-use