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The World’s #1 HPLC Column
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TABLE OF CONTENTS
Overview ....................................................................................... 2
Reproducibility Validated.............................................................. 4
pH Stability 1.5-10 ........................................................................ 6
Luna C18, C8, C5 ......................................................................... 8
Luna Phenyl-Hexyl...................................................................... 18
Luna Cyano [CN] ........................................................................ 20
Luna Amino [NH2] ....................................................................... 22
Luna LC/MS and ........................................................................ 24
Combinatorial Chemistry
Luna Capillary LC ....................................................................... 25
Luna Preparative LC ................................................................... 26
Bonded Phases ..........................................................................27Ordering Information ..................................................................28
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Wouldn't you like to boast about the performance of your HPLC
column? Many Luna® customers do. Our passion for manufacturing
the best HPLC columns, has resulted in Luna - one of the World's
most popular columns. Luna represents some of the highest quality
and performance standards in HPLC. Take a moment to explore
Luna, we are certain you will discover amazing HPLC column
performance like so many of our customers.
Superior Silica For Long Column Lifetimes
Silica quality is not simply the result of Luna's 99.999% purity.
Meticulous care is given to quality control over silica smoothness,
pore structure and pore consistency leading to a highly advanced
HPLC silica with:
• Low percentage of "fines" from damaged silica
particles stabilizing the column bed and leading
to lower backpressures and enhanced column
performance and lifetimes
• High column bed stability enhanced by
particle shape uniformity
Overview
Phases to separate any compound
LUNA ® : Si, C5, C8, C8(2), C18, C18(2), Phenyl-Hexyl, CN, NH2
Incredible Silica Smoothness
Luna silica is very smooth and spherical, as illustrated in the Luna
versus Zorbax® silica comparisons below. The likelihood of silica
particle shearing and breakage during bonding and packing is very
low; thus, Luna columns have high efficiencies and long column
lifetimes.
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pH Stability: 1.5 to 10.0*
Luna was one of the first columns to provide greater flexibility and
control in method development at pH extremes. More importantly,
Luna provides good peak shape independent of mobile phase pH.
Reproducibility Validated
A Materials Validation Document showing the test results for the
batch of material and the individual column accompanies each
Luna column. This data ensures column-to-column and batch-to-
batch reproducibility.
Versatile Configurations
Capillary-LC to preparative/process chromatography columns and
bulk material are available to meet any separation need.
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Reproducibility ValidatedIt is tough to meet Luna quality standards. The Tanaka plots below
illustrate the reproducibility of 13 different quality control parameters
for 9 batches of Luna material - nearly no variation is observed.
Each batch represents hours of quality control testing for over 25
different parameters. We know chromatographers rely on Luna for
rugged, reproducible methods worldwide; therefore, an extensive
Materials Validation Document (MVD) accompanying each column
confirms consistent column specifications and consistent
performance.
Reliable Performance
Almost no variation is observed among the batches of Luna. Figure
1 shows quality control test data designed to monitor the slightest
differences that may affect reproducibility - particle shape and
smoothness, porosimetry, bonding consistency and pH stability.
Figure 2 shows bonding reproducibility test data from 9 Luna
batches. Since differences in bonded phase coverage can show
selectivity changes, each Luna batch undergoes 6 tests for bonding
reproducibility.
Figure 1 - SilicaReproducibility. Batch No:
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Column to Column Reproducibility
The chromatograms in Figure 3 show consistency of inertness (blue)
and hydrophobicity (red) for Luna 5u C18(2) columns from 9 different
batches. Almost no variation is observed.
Figure 3 - Column-to-columnreproducibility for 9 batches of Luna
Figure 2 - Bonded PhaseReproducibility. Batch No:
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Luna was one of the first columns to provide an extended pH range
of 1.5 to 10* for most of its phases (Figure 4). The broad pH stability
range is partly achieved by the density of the bonded phase. Luna
C18(2) columns are exhaustively bonded to show great peak shape
independent of pH, indicating free silanols are well shielded from
the analyte compounds (Figure 5). Try Luna! It will consistently
provide good peak asymmetry across a wide pH range.
pH Stability: 1.5 - 10.0
Figure 4 - Efficiency and peak asymmetry stability at pH 10.0 and 1.5 forLUNA ® 5µ C18(2)
Hours of Exposure
Hours of Exposure
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Sharp Peaks For Basic Compounds at High pH
With Luna columns it is possible to work above the pKb of many
basic compounds, with the compound in a "neutral charge state",
thus the basic compound becomes more hydrophobic (less polar).
This can result in improved retention, peak shape, and
reproducibility. Figure 6 illustrates how a pH adjustment can be
used to alter selectivity and retention. A wide operational pH range
extending to 10.0 is useful for basic compounds.
pH 1.25
pH 5.0
pH 7.0
pH 9.0
pH 3.0
pH 10.0
Figure 6-Optimizing resolution and selectivity with mobile phase pH increases methoddevelopment flexibility.
Figure 5 - Average peak shape over pH range: Waters ®
Symmetry® 5µ C18 = 2.26, Phenomenex® LUNA ® 5µ C18(2) = 1.43
2 3 4 5 6 7 8 9
4
3
2
1
0
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Luna has found a place as one of the World's top reversed phase
columns because it ensures two important chromatographic
properties: resolution and peak shape. The high efficiencies and
bonded phase surface coverage provide for sharp peaks. Whether
you need a column for USP methods or just general method
development, Luna C18(2) and C8(2) should be your first choice
every time. The result:
• Free exposed silanols virtually eliminated
by complete bonding and endcapping
• Sharp peak shape for good method sensitivity
• pH stable from 1.5 to 10.0 for over 10000 hours
Luna C18(2), C8(2), C5
Phenomenex®
LUNA ® 5µ C18(2)
Waters®
Symmetry ® 5µ C18
Phenomenex®
LUNA ® 5µ C18(2)
Waters®
XTerra™ 5µ RP18
PEAK ASYMMETRY: A COMPARISON OF C18 COLUMNS
The proof is in the peaks - see for yourself.
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POLAR, ACIDIC DRUGS
Phenomenex® LUNA ® 3µ C18(2)Waters® Symmetry ® 3.5µ C18
NON-POLAR, BASIC DRUGS
Phenomenex® LUNA ® 5µ C8 Agilent Technologies® Zorbax® 5µ XDB-C8™
BASIC COMPOUNDS
Phenomenex® LUNA ® 5µ C18(2)Macherey Nagel® Nucleosil® 5µ C18
HYDROPHOBIC, ACIDIC COMPOUNDS
Phenomenex® LUNA ® 5µ C18(2)Thermo Hypersil® HyPURITY ™ Elite 5µ C18
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DIET SODA POP - AND -ACIDS IN HOP EXTRACT
CAFFEINE AND METABOLITES
1
23
4
5
0 5 10 15 20 min
METHYLATED XANTHINES
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ESTRADIOL-USP METHOD COCAINE-NARCOTIC DRUG
PHENYLEPHRINE HYDROCHLORIDE INJECTION-USP METHOD HYDROCORTISONE CREAM-USP METHOD
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0 2 4 6 8 10 12 14 16
3
2
4
5
6
7
8
9
10
11
12
13
14
1
EPA 8330 - EXPLOSIVES EPA 8310 - POLYNUCLEAR AROMATIC HYDROCARBONS (PAH’s)
COLD MEDICINE
GINGER PUNGENTS
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POLYAMINES WATER SOLUBLE VITAMINS
PHARMACEUTICAL PRESERVATIVES
PYRONES
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MINOXIDIL-USP METHOD LORAZEPAM TABLETS-USP METHOD
HORMONES
IMPIRAMINE-USP METHOD
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FATTY ACIDS
0 2 4 6 8 10 12 14
1
2
3
4 5
6
7
8
min
NAPROXEN TABLETS-USP METHOD
GUAIFENESIN TABLETS-MODIFIED USP METHOD
ALBUTEROL TABLETS-USP MEHOD
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SUN SCREENS CEPHALOSPORIN ANTIBIOTICS
PENICILLIN ANTIBIOTICS
ANTIOXIDANTS
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SAW PALMETO BERRY, P-BROMOPHENACYL ESTERS ACETAMINOPHEN, USP METHOD
CAPSAICINCYCLOSPORIN - IMMUNOSUPPRESSANTS
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Luna Phenyl-HexylEngineered for Stability
The patented Luna Phenyl-Hexyl is a reproducible, extremely stable
phenyl phase. Most other phenyl phases use a short propyl (3
carbon) linker, which limits the phase stability. This patented phase
uses a hexyl (6 carbon) linker to attach the phenyl group to the
silica surface. The result:
• Highly reproducible phenyl phase
• Dual selectivity of both phenyl phase
and a short alkyl phase
(such as a C8)• Excellent retention of amine and polar
aromatic compounds
• 1.5 to 10 pH stability for over 6000 hours
ANTIBACTERIA LS: COMPARISON OF PHENYL COLU MNS
5 10 15 20 min
1
2
3
4
5
6
7
8
Waters® Spherisorb® 5µ Phenyl
Phenomenex® LUNA ® 5µ Phenyl-Hexyl Agilent Technologies® Zorbax® 5µ Phenyl
Agilent Technologies® Zorbax® 5µ SB-Phenyl
2.5 5.0 7.5 10.0 12.5 15.0 17.5 min
1, 2
3
4
7
5,6
8
20.0
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FOOD ADDITIVESCOUGH AND COLD-USP METHOD
CHLOROBENZENES COUGH AND COLD MEDICINE
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Proven Reproducibility
Luna CN was developed to provide reproducibile chromatography
from run-to-run, column-to-column and batch-to-batch. Luna’s
high-purity silica provides a ridged and dense column bed that
allows for improved CN bonding techniques to make a stable CN
phase. The result:
• One of the most stable CN columns under both reversed
phase or normal phase conditions.
• Reproducible from run-to-run, column-to-column,
batch-to-batch.• pH stable from 1.5 to 7.0
Luna Cyano (CN)Column-to-Column Reproducibility Batch-to-Batch Reproducibility
PHTHALATE ESTERS: A COMPARSION OF CN COLUMNS
Phenomenex® LUNA ® 5µ CN Waters® Spherisorb® 5µ CN Agilent Technologies®
Zorbax® 5µ SB-CN
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ORGANOCHLORINE PESTICIDES BENZALKONIUM CHLORIDE
RHODAMINE DYES
EXPLOSIVES
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Developed for Ruggedness
Luna Amino was developed to provide improved amino column
lifetime. Column life for most amino columns can be problematic
as the amino bonding easily strips off the silica. Luna Amino,
however, shows good bonded phase stability under both normal
and reversed phase modes and across a pH range of 1.5 to 11.0.
Such a broad pH range indicates the bonded phase ruggedness
and the density of the bonded phase coverage. The result:
• Long lifetimes and low phase bleed for more
reproducible methods• Excellent retention of simple sugars, complex
sugars, sugar alcohols by reverse phase conditions
and hydrogen bonding compounds under
normal phase conditions
• pH stable from 1.5 to 11.0
• Stable in 100% aqueous mobile phases
Luna Amino (NH2 )
SIMPLE SUGARS
STABILITY IN 100% AQUEOUS MOBILE PHASE
STABILITY AT PH 11.0
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SUGAR ALCOHOLS
NUCLEIC ACID BASES
COMPLEX SUGARS
STEROIDS
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LC/MS & Combinatorial Chemistry Luna materials are highly recommended for LC/MS and high-
throughput screening. Due to Luna’s stability from pH 1.5 to 10, a
broad range of volatile, MS-compatible mobile phase modifiers
are available for LC/MS applications. High loading capacity is
possible due to high pore volume and optimum pore size distribution
of Luna silica. Our proprietary bonding procedure produces uniform
bonded phase coverage excellent for rapid mobile phase re-
equilibration. The result:
• Sharp peak shape
• High loading capacity• Low phase bleed
• Rapid re-equilibration
PEAK SYMMETRY IN MS-COMPATIBLE BUFFERS
PERFORMANCE IN MS-COMPATIBLE BUFFER
LC/MS ANALYSIS OF ANTICONVULSANTS
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Capillary ColumnsHigh Efficiency and High Sensitivity
Luna capillary columns have the same characteristics as their
respective analytical-size columns but provide added sensitivity
for microliter sample volumes. The incredible smoothness and
sphericity of Luna silica results in a high efficiency capillary-LC
column with a stable column bed that yield long column life. Our
high-purity silica combined with proprietary bonding techniques
produce a dense bonded phase shielding against silanol
interactions. With little secondary silanol interaction, Luna capillary-
LC columns yield terrific peak shape, an essential criteria for mass
limited samples.
The result:
• High efficiency 0.30, 0.50 and 0.70mm ID
glass-lined columns
• Exceptional sensitivity for low volume and mass
limited samples
• Consistent performance under LC/MS compatible
and universal gradient mobile phase conditions
• 1 / 8 inch (3mm) OD tube for robust, durable columns
-LACTOGLOBULIN TRYPTIC DIGEST
-LACTOGLOBULIN (OXIDIZED) TRYPTIC DIGEST
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Less Material Required
Luna materials have extremely low particle densiti es meaning less
material is required to pack a given volume. For large-scale
preparative systems, such as production or process methods, this
can have an enormous impact on long-term operating costs. For
example, packing a 50L column would require up to 20% less Luna
media than the leading competitive media.
Exceptional Lifetimes
The extraordinary silica and bonding technology at the heart of
Luna provides exceptional lifetime. The rigid silica resists crushing,improving the chromatographic performance over the life of the
column.
Quick, Direct Scale-Up
Luna preparative media can provide nearly the same
chromatographic behavior as a Luna analytical column. Method
scale-up is simple. Luna 3µ C18(2) material used in the analytical
separation can be switched to Luna 15µ C18(2) material to obtain
similar separation and resolution with minimal changes in the
separation method. Furthermore, the same smooth silica surface,
high bonded phase coverage and pH stability available in analytical
Luna materials is inherently present in all preparative Luna materials.
High Loadability
Luna packing materials provide high surface area silica withoptimized pore size and pore size distribution and a high pore
volume that allows extremely high loading capacity.
Preparative LC
50 L
20L
5L
0.5L
36.4 kg
27.2 kg
14.6 kg
10.9 kg
3.6 kg
0.7 kg
2.7 kg
0.5 kg
0.4 kg
0.3 kg
Column Volume BulkMaterial
20% LESSMATERIAL REQUIRED
Luna® 10u C18(2) 100A
Leading 10u C18 100A
1L
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Bonded Phases
C8(2)
Optimized for maximum efficiency, peak shape and resolution.
Significantly improved performance over traditional C8 phases
due to high surface coverage. Slightly lower carbon load thanoriginal Luna C8.
C5
Reversed-phase chemistry that offers greater hydrolytic stability
and an alternative selectivity over the classic C4 phase.
Highly hydrophobic compounds. Good alternative
to C8 or C18 when less retention is desired.
Great starting point for methods development of
pharmaceuticals, nucleotides and polar compounds.
Excellent for highly aqueous and LC/MS applications.
C8
Original C8 phase. Highly retentive; optimized for long lifetime at
extremely high pH levels (pH >10.0).
A ”workhor se” for extre mely high pH appl icat ions
(pH >10.0), or when a more retentive C8 is desired.
C18(2)
Optimized for maximum efficiency, excellent peak shape and
resolution; slightly lower carbon load than original Luna C18.
C18
Original LUNA C18 phase. Maximum retention, optimized for long
lifetime at extremely high pH levels (pH >10.0)
A ”workhorse” for extremely high pH applicat ions
(>10.0), or when maximum retention is desired.
Phenyl-Hexyl
Novel (patented) phenyl phase which employs a hexyl linker, as
opposed to the traditional propyl chain. Stable under all conditions
as well as alternative selectivity to most phenyl phases.
Unique selectivity for very polar and aromatic
compounds when used in methanol containing mobile
phases.
CN
One of the best performing CN phases available. Can be used in
reversed- or normal-phase modes. Based on same 99.999% pure
silica as all Luna phases.
Excellent for improving the retention of polar
compounds. Extremely rapid equilibration makes it
ideal for rapid screening and gradient applications.
A C18 phase for virtuall y all HPLC appl icat ions .
Range of particle sizes offers amazing versatility for
capillary-LC/MS, to prep and process scale applications.
DESCRIPTION APPLICATION
NH2
Possibly the most rugged and reproducible NH2phase for reversed
or normal phase separations. Stable from pH 1.5 to 11.0 and
under 100% aqueous conditions.
Excellent for reversed-phase analysis of sugars, sugar
alcohols and anionic compounds or for hydrogen
bonding compounds under normal phase.
LUNA PHASE Particle Pore Surface Carbon Bonded pH StabilitySize (µ) Size Area Load Phase
(Å) (m2 /g) (%) Coverage(µmole/m2)
Silica(2) 3,5,10,15 100 400 - - 2.0 - 7.5
C5 5,10 100 440 12.5 7.85 1.5 - 10
C8 5,10 100 440 14.75 5.50 1.5 - 10
C8(2) 3,5,10, 100 400 13.5 5.50 1.5 - 10
10-PREP,15
C18 5,10 100 440 19.0 3.00 1.5 - 10
C18(2) 3,5,10, 100 400 17.5 3.00 1.5 - 10
10-PREP,15
Phenyl-Hexyl 3,5,10, 100 400 17.5 4.00 1.5 - 10
10-PREP,15
CN 3,5,10 100 400 7.0 3.80 1.5 - 7.0
NH2
3,5,10 100 400 9.5 5.80 1.5 - 11
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5µ Microbore and MiniboreColumns (mm)
Phases 50 x 1.0 150 x 1.0 250 x 1.0 30 x 2.0 50 x 2.0 150 x 2.0 250 x 2.0 *4 x 2.0mm **4 x 3.0mm
Silica(2) — — — — 00B-4274-B0 00F-4274-B0 00G-4274-B0 AJ0-4347 AJ0-4348
C5 — — — 00A-4043-B0 00B-4043-B0 00F-4043-B0 — AJ0-4292 AJ0-4293
C8 — — — 00A-4040-B0 00B-4040-B0 00F-4040-B0 00G-4040-B0 AJ0-4289 AJ0-4290
C8 (2) 00B-4249-A0 00F-4249-A0 00G-4249-A0 00A-4249-B0 00B-4249-B0 00F-4249-B0 00G-4249-B0 AJ0-4289 AJ0-4290
C18 — — — 00A-4041-B0 00B-4041-B0 00F-4041-B0 00G-4041-B0 AJ0-4286 AJ0-4287
C18 (2) 00B-4252-A0 00F-4252-A0 00G-4252-A0 00A-4252-B0 00B-4252-B0 00F-4252-B0 00G-4252-B0 AJ0-4286 AJ0-4287
CN — — — 00A-4255-B0 00B-4255-B0 00F-4255-B0 00G-4255-B0 AJ0-4304 AJ0-4305
NH2 — 00F-4378-A0 00G-4378-A0 00A-4378-B0 00B-4378-B0 00F-4378-B0 00G-4378-B0 AJ0-4301 AJ0-4302
P he ny l-H ex yl 00 B- 425 7-A 0 00F -4 25 7-A 0 — 0 0A -4 257 -B 0 00 B-4 257 -B0 0 0F -4 25 7- B0 00 G- 425 7-B 0 A J0 -4 350 AJ 0- 43 51
SecurityGuard™Cartridges require universal holder Order No.: KJO-4282
ORDERING INFORMATION
*for column ID 2.0-3.0 -**for column ID 3.2-8.0
SecurityGuard
3µ AnalyticalColumns (mm)
Phases 30 x 3.0 50 x 3.0 150 x 3.0 30 x 4.6 50 x 4.6 75 x 4.6 100 x 4.6 150 x 4.6 *4 x 2.0mm **4 x 3.0mm
Silica(2) — — 00F-4162-Y0 — 00B-4162-E0 — 00D-4162-E0 00F-4162-E0 AJ0-4347 AJ0-4348
C8(2) 00A-4248-Y0 00B-4248-Y0 00F-4248-Y0 00A-4248-E0 00B-4248-E0 00C-4248-E0 00D-4248-E0 00F-4248-E0 AJ0-4289 AJ0-4290
C18 (2 ) 00A-4251-Y0 00B-4251-Y0 00F-4251-Y0 00A-4251-E0 00B-4251-E0 00C-4251-E0 00D-4251-E0 00F-4251-E0 AJ0-4286 AJ0-4287
C N — 0 0B -4 254 -Y 0 0 0F -4 254 -Y0 00A -4 25 4- E0 0 0B -4 254 -E0 00 C- 425 4-E 0 00 D- 42 54- E0 0 0F -4 254 -E 0 A J0- 430 4 A J0 -4 30 5
NH2 — 00B-4377-Y0 00F-4377-Y0 00A-4377-E0 00B-4377-E0 00C-4377-E0 00D-4377-E0 00F-4377-E0 AJ0-4301 AJ0-4302Pheny l-Hexy l — 00B-4256-Y0 00F-4256-Y0 00A-4256-E0 00B-4256-E0 00C-4256-E0 00D-4256-E0 00F-4256-E0 AJ0-4350 AJ0-4351
SecurityGuard™Cartridges require universal holder Order No.: KJO-4282
SecurityGuard
3µ Microbore and MiniboreColumns (mm)
Phases 30 x 1.0 50 x 1.0 150 x 1.0 30 x 2.0 50 x 2.0 100 x 2.0 150 x 2.0 *4 x 2.0mm **4 x 3.0mm
Silica(2) — — — 00A-4162-B0 00B-4162-B0 00D-4162-B0 00F-4162-B0 AJ0-4347 AJ0-4348
C8(2) 00A-4248-A0 00B-4248-A0 00F-4248-A0 00A-4248-B0 00B-4248-B0 00D-4248-B0 00F-4248-B0 AJ0-4289 AJ0-4290
C18 (2 ) 00A-4251-A0 00B-4251-A0 00F-4251-A0 00A-4251-B0 00B-4251-B0 00D-4251-B0 00F-4251-B0 AJ0-4286 AJ0-4287
CN — — — 00A-4254-B0 00B-4254-B0 00D-4254-B0 00F-4254-B0 AJ0-4304 AJ0-4305
NH2
— — 00 F- 43 77- A0 00 A-4 377 -B 0 00B -4 377 -B0 0 0D -4 377 -B0 0 0F -43 77- B0 A J0- 43 01 A J0 -4 302
P he ny l-H ex yl 0 0A -4 256 -A 0 00B -4 25 6- A0 — 00 A-4 256 -B0 0 0B- 42 56- B0 00 D- 42 56 -B 0 0 0F -4 256 -B0 AJ 0- 43 50 A J0 -4 35 1
SecurityGuard™Cartridges require universal holder Order No.: KJO-4282
SecurityGuard
3µ and 5µ Capillary Columns (mm)
Phases 50 x 0.30 150 x 0.30 250 x 0.30 50 x 0.50 150 x 0.50 250 x 0.50 50 x 0.70 150 x 0.70 250 x 0.70
3µ C8(2) 00B-4248-AC 00F-4248-AC 00G-4248-AC 00B-4248-AF 00F-4248-AF 00G-4248-AF 00B-4248-AG 00F-4248-AG 00G-4248-AG
3µ C18(2) 00B-4251-AC 00F-4251-AC 00G-4251-AC 00B-4251-AF 00F-4251-AF 00G-4251-AF 00B-4251-AG 00F-4251-AG 00G-4251-AG
5µ C8(2) 00B-4249-AC 00F-4249-AC 00G-4249-AC 00B-4249-AF 00F-4249-AF 00G-4249-AF 00B-4249-AG 00F-4249-AG 00G-4249-AG
5µ C18(2) 00B-4252-AC 00F-4252-AC 00G-4252-AC 00B-4252-AF 00F-4252-AF 00G-4252-AF 00B-4252-AG 00F-4252-AG 00G-4252-AG
If Luna does not provide at leastan equivalent separation as com-pared to a competing column of thesimilar particle size, phase and
dimensions, send in your comparative data within 45 days and keep theLuna column for FREE.
28 Phenomenex Tel. 310.212.0555 Fax 310.328.7768 Web www.phenomenex .com
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10µ Bulk Packings
Phase 100g 1kg 5kg 10kg 50kg 100kg
Silica(2) 04G-4091 04K-4091 04L-4091 04M-4091 04N-4091 04P-4091
Bonded Silica
C5 04G-4092 04K-4092 04L-4092 04M-4092 04N-4092 04P-4092
C8 04G-4093 04K-4093 04L-4093 04M-4093 04N-4093 04P-4093
C8(2) 04G-4250 04K-4250 04L-4250 04M-4250 04N-4250 04P-4250
C18 04G-4094 04K-4094 04L-4094 04M-4094 04N-4094 04P-4094
C18(2) 04G-4253 04K-4253 04L-4253 04M-4253 04N-4253 04P-4253
CN 04G-4300 04K-4300 04L-4300 04M-4300 04N-4300 04P-4300
NH2 04G-4379 04K-4379 04L-4379 04M-4379 04N -4379 04P-4379Phenyl-Hexyl
Phenyl-H exyl 04G-4285 04K-4285 04L-4285 04M-4285 04N-4285 04P-428510µ vs. 10µ- PREP :
What is the difference?
The Luna 10µ and 10µ-PREP differ by only
one factor: Particle size distribution. The
silica matrix and bonding chemistry are
identical, but the 10µ-PREP has a wider
particle size distribution to reduce cost.
Which should I choose?
Luna 10µ-PREP offers exceptional
performance, suitable for most appli-
cations, with excellent economy. Luna 10µ
is available when the absolute best prep-arative performance is critical. Extremely
narrow size distribution yields the highest
efficiency and maximum resolution.
Evaluation is the Best Test
All Luna materials are available in scout
columns. Contact your Phenomenex
representative to set up an evaluation.
10µor
10µ-PREP ?
10µ- PREP Bulk Packings
Phase 100g 1kg 5kg 10kg 50kg 100kg
Silica(2) 04G-4322 04K-4322 04L-4322 04M-4322 04N-4322 04P-4322
Bonded Silica
C8(2) 04G-4323 04K-4323 04L-4323 04M-4323 04N-4323 04P-4323
C18(2) 04G-4324 04K-4324 04L-4324 04M-4324 04N-4324 04P-4324
Phenyl-Hexyl
Phenyl-H exyl 04G-4325 04K-4325 04L-4325 04M-4325 04N-4325 04P-4325
15µ Bulk Packings
Phase 100g 1kg 5kg 10kg 50kg 100kg
Silica(2) 04G-4271 04K-4271 04L-4271 04M-4271 04N-4271 04P-4271
Bonded Silica
C8(2) 04G-4272 04K-4272 04L-4272 04M-4272 04N-4272 04P-4272
C18(2) 04G-4273 04K-4273 04L-4273 04M-4273 04N-4273 04P-4273
Phenyl-Hexyl
Phenyl-H exyl 04G-4286 04K-4286 04L-4286 04M-4286 04N-4286 04P-4286
ORDERING INFORMATION
15µ Preparative and Pilot ScaleColumns (mm)
Phases 250 x 4.6 250 x 21.2 250 x 30 250 x 50 250 x 100 50 x 21.2 50 x 50
Sil ica(2) 00G-4271-E0 00G-4271-P0 00G-4271-U0 00G-4271-V0 00G-4271-W0 03B-4271-P0 03B-4271-V0
C8(2) 00G-4272-E0 00G-4272-P0 00G-4272-U0 00G-4272-V0 00G-4272-W0 03B-4272-P0 03B-4272-V0
C18(2) 00G-4273-E0 00G-4273-P0 00G-4273-U0 00G-4273-V0 00G-4273-W0 03B-4273-P0 03B-4273-V0
Phenyl-Hexyl 00G-4286-E0 00G-4286-P0 00G-4286-U0 00G-4286-V0 00G-4286-W0 03B-4286-P0 03B-4286-V0
Guard(mm)
Phenomenex Tel. 310.212.0555 Fax 310.328.7768 Web www.phenomenex.co m
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Guard Cartridge System
Order No. Replacement Parts Unit Price
AJ0-4283 PEEK Ferrules 3/pk
AJ0-4285 Stacking Rings 2/pk
AQ0-1389 PEEK (Biocompatible) 10/pk Fingertight fittings
AJ0-4284 SecurityGuard™ Wrenches 2/pk
Method Development Kits
Order No. Description Dimensions(mm) Unit Price
K H0 -4 760 L un a M eth od D ev elo pme nt K it ( 5 co lu mn s) 1 50 x 4. 6 5 /p k 1 each of 5µ C18(2), C8(2), C5, CN, Phenyl-Hexyl Phases
KH0-4354 Luna Rapid-MD Kit (3 columns)1 eac h o f 3µ C18 (2 ), C8(2) a nd Pheny l-Hexy l pha ses 50 x 4 .6 3 /pk
KHO-4761 Luna Selectivity Kit 1 (3 columns)1 e ach o f 5 µ C 18( 2) , P he ny l- He xy l, CN ph as es 15 0 x 4 .6 3 /p k
KH0-4762 Luna Selectivity Kit 2 (3 columns)
1 e ac h o f 5 µ C 8( 2) , P he ny l- He xy l, CN p has es 15 0 x 4 .6 3 /p k
Method Validation Kits
Order No. Description Dimensions (mm) Unit Price
KH0-4355 Luna 5µ C18 Method Validation Kit 150 x 4.6 3/pk
KH0-4356 Luna 5µ C18(2) Me thod Validation Kit 150 x 4.6 3/pk
KH0-4357 Luna 5µ C8 Method Validation Kit 150 x 4.6 3/pk
KH0-4358 Luna 5µ C8(2) Method Validation Kit 150 x 4.6 3/pk
K H0 -4 359 L un a 5 µ P he ny l-H ex yl M eth od Val id at io n K it 1 50 x 4 .6 3 /p k
Holder Assembly Kit
Order No. Description Price
KJO-4282 Guard Cartridge Kit.Includes: 1 cartridgeholder, 3 PEEK ferrules,2 wrenches, 2 stackingrings, 2 PEEK fingertight fittings
ORDERING INFORMATION
ORDERING INFORMATION
Method
Development KitsThe Luna Method Development Kits
combine the incredible performance of
Luna HPLC columns with three different
selectivities. Both kits include a Luna
Phenyl-Hexyl (moderate polarity), a Luna
CN (polar), and your choice of a C8(2) or
C18(2) (non-polar). A wide range of
selectivies for virtually any separation
challenge.
Validation Kits
Three columns of the same phase from
three different batches provide an excellent
benchmark for system validation. Method
reprodicibility is easily and quickly
assessed, clearly demonstrating
chromatographic performance, validation
potential and long term compliance with
Luna Method Validation Kits.
CartridgesCartridges for General Purpose/Pharmaceutical
Order No. Material Dimensions Column ID pH Stability Unit PriceDescription (mm)
AJ0-4286 C18 4 x 2.0 2.0 - 3.0 mm 1.5 - 10 10/pk
AJ0-4287 C18 4 x 3.0 3.1 - 8.0 mm 1.5 - 10 10/pk AJ0-4289 C8 4 x 2.0 2.0 - 3.0 mm 1.5 - 10 10/pk
AJ0-4290 C8 4 x 3.0 3.1 - 8.0 mm 1.5 - 10 10/pk
AJ0-4292 C5 4 x 2.0 2.0 - 3.0 mm 1.5 - 10 10/pk
AJ0-4293 C5 4 x 3.0 3.1 - 8.0 mm 1.5 - 10 10/pk
AJ0-4347 Silica 4 x 2.0 2.0 - 3.0 mm — 10/pk
AJ0-4348 Silica 4 x 3.0 3.1 - 8.0 mm — 10/pk
AJ0-4350 Phenyl 4 x 2.0 2.0 - 3.0 mm 1.5 - 10 10/pk
AJ0-4351 Phenyl 4 x 3.0 3.1 - 8.0 mm 1.5 - 10 10/pk
AJ0-4304 CN 4 x 2.0 2.0 - 3.0 mm 1.5 - 7 10/pk
AJ0-4305 CN 4 x 3.0 3.1 - 8.0 mm 1.5 - 7 10/pk
AS0-4301 NH2
4 x 2.0 2.0 - 3.0mm 1.5 - 11 10/pk
AS0-4302 NH2
4 x 3.0 3.1 - 8.0mm 1.5 - 11 10/pk
Phenomenex Tel. 310.212.0555 Fax 310.328.7768 Web www.phenomene x.com
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5µ AnalyticalColumns (mm)
Phases 30 x 3.0 50 x 3.0 150 x 3.0 250 x 3.0 30 x 4.6 50 x 4.6 75 x 4.6 *4 x 2.0mm **4 x 3.0mm
Silica(2) — — — — — 00B-4274-E0 — AJ0-4347 AJ0-4348
C5 — — 00F-4043-Y0 00G-4043-Y0 — 00B-4043-E0 — AJ0-4292 AJ0-4293
C8 — — 00F-4040-Y0 00G-4040-Y0 00A-4040-E0 00B-4040-E0 00C-4040-E0 AJ0-4289 AJ0-4290
C8(2) 00A-4249-Y0 00B-4249-Y0 00F-4249-Y0 00G-4249-Y0 00A-4249-E0 00B-4249-E0 00C-4249-E0 AJ0-4289 AJ0-4290C18 — — 00F-4041-Y0 00G-4041-Y0 0 0A-4041-E0 0 0B-4041-E0 0 0C-4041-E0 AJ0-4286 AJ0-4287
C18 (2 ) 00A-4252-Y0 00B-4252-Y0 00F-4252-Y0 00G-4252-Y0 00A-4252-E0 00B-4252-E0 00C-4252-E0 AJ0-4286 AJ0-4287
CN — 00B-4255-Y0 00F-4255-Y0 00G-4255-Y0 00A-4255-E0 00B-4255-E0 00C-4255-E0 AJ0-4304 AJ0-4305
NH2
— 00B-4378-Y0 00F-4378-Y0 00G-4378-Y0 00A-4378-E0 00B-4378-E0 00C-4378-E0 AJ0-4301 AJ0-4302
Phenyl-Hexyl 00A-4257-Y0 00B-4257-Y0 00F-4257-Y0 00G-4257-Y0 00A-4257-E0 00B-4257-E0 00C-4257-E0 AJ0-4350 AJ0-4351
SecurityGuard™Cartridges require universal holder Order No.: KJO-4282
5µ Analytical, Semi-Prep and PreparativeColumns (mm)
Phases 100 x 4.6 150 x 4.6 250 x 4.6 250 x 10 250 x 15 250 x 21.2 250 x 30 50 x 10 *4 x 2.0mm **4 x 3.0mm
Sil ica(2) 00D-4274-E0 00F-4274-E0 00G-4274-E0 00G-4274-N0 00G-4274-AK 00G-4274-P0 00G-4274-U0 03B-4274-N0 AJ0-4347 AJ0-4348
C5 00D-4043-E0 00F-4043-E0 00G-4043-E0 00G-4043-N0 — — — — AJ0-4292 AJ0-4293
C8 00D-4040-E0 00F-4040-E0 00G-4040-E0 00G-4040-N0 — — — 03B-4040-N0 AJ0-4289 AJ0-4290
C8(2) 00D-4249-E0 00F-4249-E0 00G-4249-E0 00G-4249-N0 00G-4249-AK 00G-4249-P0 00G-4249-U0 03B-4249-N0 AJ0-4289 AJ0-4290
C18 00D-4041-E0 00F-4041-E0 00G-4041-E0 00G-4041-N0 — — — 03B-4041-N0 AJ0-4286 AJ0-4287
C18 (2 ) 00D-4252-E0 00F-4252-E0 00G-4252-E0 00G-4252-N0 00G-4252-AK 00G-4252-P0 00G-4252-U0 03B-4252-N0 AJ0-4286 AJ0-4287
CN 00D-4255-E0 00F-4255-E0 00G-4255-E0 00G-4255-N0 — — — 03B-4255-N0 AJ0-4304 AJ0-4305
NH2
00D-4378-E0 00F-4378-E0 00G-4378-E0 00G-4378-N0 — –- — 03B-4378-N0 A J0-4301 A J0-4302
Phenyl-Hexyl 00D-4257-E0 00F-4257-E0 00G-4257-E0 00G-4257-N0 00G-4257-AK 00G-4257-P0 00G-4257-U0 03B-4257-N0 AJ0-4350 AJ0-4351
SecurityGuard™Cartridges require universal holder Order No.: KJO-4282
Guard (mm) SecurityGuardCartridges 10/pk
10µ Semi-Prep and PreparativeColumns (mm)
Phases 250 x 4.6 250 x 10 250 x 15 250 x 21.2 250 x 30 250 x 50 250 x 100 50 x 10 50 x 21.2 50 x 50 *4 x 2.0mm **4 x 3.0mm
Sil ica(2) 00G-4091-E0 00G-4091-N0 00G-4091-AK 00G-4091-P0 00G-4091-U0 00G-4091-V0 00G-4091-W0 03B-4091-N0 03B-4091-P0 03B-4091-V0 AJ0-4347 AJ0-4348
C5 00G-4092-E0 00G-4092-N0 — 00G-4092-P0 — 00G-4092-V0 00G-4092-W0 03B-4092-N0 03B-4092-P 0 03B-4092-V0 AJ0-4292 AJ0-4293
C8 00G-4093-E0 00G-4093-N0 — 00G-4093-P0 — 00G-4093-V0 00G-4093-W0 03B-4093-N0 03B-4093-P 0 03B-4093-V0 AJ0-4289 AJ0-4290
C8(2) 00G-4250-E0 00G-4250-N0 00G-4250-AK 00G-4250-P0 00G-4250-U0 00G-4250-V0 00G-4250-W0 03B-4250-N0 03B-4250-P0 03B-4250-V0 AJ0-4289 AJ0-4290
C18 00G-4094-E0 00G-4094-N0 — 00G-4094-P0 00G -4094-V0 00G-4094-W0 03B-4094-N0 03B-4094-P0 03B-4094-V0 AJ0-4286 A J0-4287
C18(2) 00G-4253-E0 00G-4253-N0 00G-4253-AK 00G-4253-P0 00G-4253-U0 00G-4253-V0 00G-4253-W0 03B-4253-N0 03B-4253-P0 03B-4253-V0 AJ0-4286 AJ0-4287
NH2
00G-4379-E0 00G-4379-N0 00G-4379-AK 00G-4379-P0 0 0G-4379-U0 0 0G-4379-V0 00G-4379-W0 — — — — —
Phenyl-Hexyl 00G-4285-E0 00G-4285-N0 00G-4285-AK 00G-4285-P0 00G-4285-U0 00G-4285-V0 00G-4285-W0 03B-4285-N0 03B-4285-P0 03B-4285-V0 AJ0-4350 AJ0-4351SecurityGuard™Cartridges require universal holder Order No.: KJ0-4282
SecurityGuardCartridges 10/pk
SecurityGuardCartridges 10/pk
ORDERING INFORMATION
5u Columns for High-Throughput PurificationColumns (mm)
Phases 50 x 21.2 100 x 21.2 150 x 21.2 50 x 30 75 x 30 100 x 30
Sil ica(2) 00B-4274-P0 00D-4274-P0 00F-4274-P0 00B-4274-U0 00C-4274-U0 00D-4274-U0
C8(2) 00B-4249-P0 00D-4249-P0 00F-4249-P0 00B-4249-U0 00C-4249-U0 00D-4249-U0
C18 (2 ) 00B-4252-P0 00D-4252-P0 00F-4252-P0 00B-4252-U0 00C-4252-U0 00D-4252-U0
*for column ID 2.0-3.0 -**for column I D 3.2 -8.0
Guard (mm)
Phenomenex Tel. 310.212.0555 Fax 310.328.7768 Web www.phenomene x.com
2320 W 205th St
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U.S.A. PUERTO RICO CANADA UNITED KINGDOM GERMANY NEW ZEALAND AUSTRALIA
TEL: (310) 212-0555 (800) 541-HPLC (800) 543-3681 01625-501367 06021-58830-0 09-4780951 1800 553 929
FAX: (310) 328-7768 (310) 328-7768 (310) 328-7768 01625-501796 06021-58830-11 09-4780952 1800 553 923
Luna® is available worldwide. For the distributor in your country contact Phenomenex USA,
International Department by telephone, fax or e-mail: [email protected]
®
The World’s #1 HPLC Column
2320 W. 205th St.
Torrance, CA 90501-1456
U.S.A.
P E B 2 0 1 8 1 I NT - 0