158 Phenomenex HPLC Luna TM LUNA ® Try Gemini ® C18 for even longer column lifetimes (see page 126). Luna ® is a registered trademark of Phenomenex, Inc. Material Characteristics Packing Particle Pore Surface Carbon Calculated Bonded pH Material Shape/Size Size Area Load Phase Coverage Stability (μm) (Å) (m 2 /g) % (µmole/m 2 ) Luna Silica(2) † Spher. 3, 5,10,15 100 400 0 – 1.5 - 7.0 Luna C5 Spher. 5,10 100 440 12.5 7.85 1.5 - 10 Luna C8 Spher. 5,10 100 440 14.75 5.50 1.5 - 10 Luna C8(2) Spher. 3, 5,10,15 100 400 13.5 5.50 1.5 - 10 Luna C18 Spher. 5,10 100 440 19 3.00 1.5 - 10 Luna C18(2) Spher. 2.5, 3, 5,10,15 100 400 17.5 3.00 1.5 - 10 Luna Phenyl-Hexyl Spher. 3, 5,10, 15 100 400 17.5 4.00 1.5 - 10 Luna CN † Spher. 3, 5, 10 100 400 7.0 3.80 1.5 - 7.0 Luna NH 2 † Spher. 3, 5, 10 100 400 9.5 5.80 1.5 - 11 Luna SCX † Spher. 5,10 100 400 Binding Capacity: 0.15 meq/g 2.0 - 7.0 USP Phases for Virtually Every Application Luna Bonded Phase Selectivity Chart ONE OF THE WORLD'S LEADING HPLC COLUMNS Develop or Improve your HPLC Method • Wide pH stability from 1.5 to 10 † • Exceptionally rugged USP Phases: Silica, C5, C8, C18, Phenyl-Hexyl, CN, NH 2 , SCX • Easy method scalability with 3, 5, 10, 10-PREP and 15 μm medias • Extensive batch traceability and reproducibility data supplied with every column USP Column Classification Phase Description Common Applications L1 C18(2) 2.5, 3, 5, 10, 10-PREP, 15µm C18 phase. Excellent efficiency, peak shape and resolution. Slightly lower carbon load than original Luna C18. Acetaminophen, Aspirin, Caffeine, Albuterol, Amitriptyline Hydrochloride, Amoxicillin, Atenolol, Cephalexin, Cephradine capsules, Chloramphenicol, Cortisone Acetate, Dextromethorphan, Diphenhydramine, Pseudoephedrine, Dopamine, Estradiol, Guaifenesin, Ibuprofen, Sterile Imipenem, Imipramine, Lidocaine, Lorazepam, Minoxidil, Naproxen, Phenylephrine Hydrochloride, Phenylpropanolamine, Prednisone oral solution, Procainamide, Propoxyphene, Reserpine L3 Silica(2) 3, 5, 10µm Ultra-pure silica with high column bed stability enhanced by particle shape uniformity. Alprazolam, Hydrocodone bitartrate, Hydrocortisone, Fat Soluble Vitamins, Phthalates, Fatty Acids, Lutein, Lycopene, Estradiol L7 C8(2) 3, 5, 10, 10-PREP, 15µm C8 phase for excellent efficiency, peak shape and resolution. Significantly improved performance over traditional C8 phases due to high surface coverage. Doxepine, Doxylamine succinate, Fluoxetine, Glyburide, Ibuprofen Oral Suspension, Propranolol, Levonorgestrel, Ethinyl estradiol, Melengestrol acetate, Glucosamine L8 NH 2 3, 5, 10µm Amino phase. Can be used in reversed or normal phase modes. Stable from pH 1.5 to 11.0 and under 100% aqueous conditions. High performance silica and bonding techniques produces a rugged, highly reproducible column. Simple sugars, Carboplatin, Lactulose concentrate, Levocarnitine tablets L9 SCX 5, 10µm A Benzene Sulfonic Acid bonded phase is used to make this Strong Cation Exchange (SCX) column. Offers great peak shape and resolution. Cough and cold compounds, Raclopride, Sodium Acetate, Erythromycin L10 CN 3, 5, 10µm Cyano phase. Can be used as reversed- or normal-phase material. The use of Luna base silica results in overall phase reproducibility and performance. Benzalkonium Chloride, Nortriptyline HCl Capsules, Prednisolone, Tetracaine, Quinapril tablets L11 Phenyl- Hexyl 3, 5, 10, 10-PREP,15µm A phenyl phase which employs a hexyl alkyl linker as opposed to the traditional propyl chain. Offers great stability as well as alternative selectivity. Oxacillin,Captopril, Chlorpheniramine, Pseudoephedrine, Methadone Hydrochloride Oral Concentration If Luna does not provide at least an equivalent separation as compared to a competing column of the same particle size, similar phase and dimensions, send in your comparative data within 45 days and keep the Luna column for FREE
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158 Phenomenex
HPLC
Luna
TM
Luna®
Try Gemini® C18 for even longer column lifetimes (see page 126).
Luna® is a registered trademark of Phenomenex, Inc.
Material CharacteristicsPacking Particle Pore Surface Carbon
CalculatedBonded pH
Material Shape/Size Size Area Load PhaseCoverage Stability (μm) (Å) (m2/g) % (µmole/m2)
If Luna does not provide at least anequivalentseparationascomparedtoacompeting columnof the sameparticlesize,similarphaseanddimensions,sendinyourcomparativedatawithin45daysandkeeptheLunacolumnforFREE
159Phenomenex
HPLCLuna
TM
Luna®
LUnA SiLiCA
A Backbone and Phase designed for Long Column LifetimesLuna’s excellent performance is not simply the result of ultra-pure metal-free silica (99.99 % purity). Meticulous care is given to the quality control of surface smoothness, pore structure and pore consistency to ensure particles of uniform structure and enhanced mechanical strength. Either bonded or unbonded, Luna silica produces highly advanced HPLC columns:
• Low percentage of “fines” from damaged silica leading to lower backpressures and enhanced column performance and lifetimes
• high column bed stability enhanced by particle shape uniformity
incredible Silica SmoothnessLuna silica is extremely smooth and spherical. For bonded phases, this provides a uniform bonding surface for consistent and even bonded phase coverage. The likelihood of silica particle shearing and breakage during bonding and packing is very low; thus, Luna columns have high efficiencies and long column lifetimes.
• recommended for preparative and bulk packing into dAC systems, see page 288 for more information
see p. 379 for testing probes.
160 Phenomenex
HPLC
Luna
TM
Luna®
App
ID1
100
1
2
3Pyridine Asymmetry:As 2.27
0 2 4 6 8 10 12 min
0
100
200
300
1
2
3
Pyridine Adsorption:No elution
0 2 4 6 8 10 12 min
0
100
200
300
400
500
1
2
3
0 2 4 6 8 10 12 min
0
100
200
300
400 Pyridine Asymmetry:As 1.44
Zorbax® 5 μm C18
Symmetry® 5 μm C18
Luna® 5 μm C18(2)
Peak asymmetry Comparison of Competing Columns**
Conditions for all columns Dimensions: 150x4.6mmMobile Phase: Acetonitrile/Water(50:50)Flow Rate: 1.0mL/minDetection: UV@254nmSample: 1. Pyridine 2. Phenol 3. Toluene
Comparison of 7 different 5 μm reversed phase columns. This survey measures the degree of silanol activity on the surface of each silica. In this survey, Luna 5 µm C18(2) material demonstrates the lowest silanol activity.
Pyridine Peak asymmetry Comparison**
LUnA C18(2), C8(2), C5
take One Small Step and a giant Leap in PerformanceLuna has found a place as one of the World’s top reversed phase columns because it provides a measurable improvement over many HPLC columns for two important chromatographic properties: resolution and peak shape. The high efficiencies and bonded phase surface coverage provide for sharp peaks. The result:
• free exposed silanols virtually eliminated by complete bonding and endcapping
Enhanced SelectivityLuna Phenyl-Hexyl provides separations not achievable on C18 or C8 columns; such as increased retention for polar, aromatic compounds as well as reversals in analyte elution order. Luna Phenyl-Hexyl is a reproducible, extremely stable phenyl phase. Most phenyl phases use a short propyl (3 carbon) linker, which limits phase stability. The Phenyl-Hexyl bonded phase employs a phenyl ring with a hexyl (6 carbon) linker and is densely bonded to Luna silica surface. Dense bonding and the hexyl linker reduce bonded phase hydrolysis and increases chemical stability. The result:
• highly reproducible and stable phenyl phase
• dual selectivity of both phenyl phase and a short alkyl phase (C5 or C8)
• Excellent retention of aromatic and polar, amine compounds
see p. 379 for testing probes.1.5-10.03μm,5μm,10μm,10μm-PREP,and15μmPhenylwithHexyl(C6)linker,endcappedNon-polarcompoundsAromaticselectivityenhancedbyhigherhydrophobicityduetohexyllinker
Enhance Aromatic Selectivity with Methanol!Luna Phenyl-Hexyl can provide separations not achievable on alkyl phases due to an increased retention for aromatic compounds. To fully express this aromatic selectivity, varying amounts of methanol can be used as the organic mobile phase in place of acetonitrile. π-π interactions between the bonded phase and different analytes can be modulated by the amounts of acetonitrile and methanol used in the mobile phase.
Proven reproducibilityFor carboxyl, carbonyl and amine containing compounds, Luna CN offers a unique polar selectivity in reversed phase and normal phase mode. Luna CN provides sharp peaks and great reproducibility run-to-run, column-to-column and batch-to-batch. Luna’s smooth silica allows for a more uniform bonding with improved resistance to bonded phase hydrolysis to produce one of the most stable CN phases. The result:
• improved peak shape
• One of the most stable Cn columns under reversed phase or normal phase conditions.
developed for ruggednessLuna NH2 retains hydrogen-bonding compounds under three separation modes: Reversed Phased, Normal Phase, and Ion Exchange. Luna NH2 provides reproducible retention and selectivity with improved column lifetime. Amino columns can be problematic as the bonded phase easily hydrolyses off the silica, shortening retention time over the life of the column. The bonded phase stability of Luna NH2 is illustrated by 1.5 to 11.0 pH stability and 100 % aqueous mobile phase stability. The result:
• increased bonded phase stability improves reproducibility and column lifetime
• Excellent retention of simple sugars, complex sugars, sugar alcohols and other hydrogen-bonding compounds under reversed Phase, normal Phase, ion Exchange conditions
Stability of Luna Amino at ph 1.5Column: Luna5µmNH2 Dimensions: 250x4.6mmPart no.: 00G-4378-E0Mobile Phase: 20mMPotassiumPhosphateBuffer pH2.7/Acetonitrile(95:5)Flow Rate: 1.5mL/minDetector: UV@254nmTemperature: AmbientStability Conditions: Continuouslyflushedat1.0mL/min using20mMPotassiumPhosphateBuffer pH1.5/Acetonitrile(95:5)betweeninjections
Stability of Luna Amino at ph 11.0Column: Luna5µmNH2Dimensions: 250x4.6mmPart no.: 00G-4378-E0Mobile Phase: 20mMPotassiumPhosphateBuffer pH2.7/Acetonitrile(95:5)Flow Rate: 1.5mL/minDetector: UV@254nmTemperature: AmbientStability Conditions: Continuously flushed at 1.0 mL/min using 20 mM Potassium Phosphate Buffer pH 11.0/Acetonitrile (95:5) between injections
Luna NH2 (cont'd)
Method flexibilityStable from pH 1.5 to 11.0, Luna Amino offers greater flexibility in method development. By adjusting the pH of the mobile phase, retention times of analytes can often be manipulated - depending on the pKa of the sample analyte. Even if extreme pH is not required, this pH stability indicates column ruggedness and generally longer column life.
LUnA SCX (StrOng CAtiOn EXChAngE)Luna SCX provides excellent resolution and peak shape of basic, cationic compounds. However, most SCX columns show poor peak shape and bad resolution causing many chromatographers to ignore this important phase for small molecule method development, until now. Luna SCX contains a benzene sulfonic acid ligand providing ion-exchange reversed phase, and aromatic interactions. Such interaction make Luna SCX great as a first dimension of 2D LC applications as well as improved resolution for small molecules. The result:
• resolving power and sharp peak shape to separate complex cationic/basic and nitrogen containing compounds
• 5 and 10 μm columns and bulk media for analytical through preparative separations
tryptic digest of Bovine Cytochrome c Column: Luna5μmSCXDimensions: 150x4.6mmPart no.: 00F-4398-E0Mobile Phase: A) 20mMPotassiumPhosphate,pH2.5/ 25%Acetonitrile B) 20mMPotassiumPhosphate,pH2.5/ 25%Acetonitrile/350mMPotassiumChlorideGradient: 100%Ato100%Bin50minutesFlow Rate: 1mL/minTemperature: 35°CDetection: UV@215nmInjection Volume: 50µL(20µgoncolumn)Sample: BovineCytochromectrypsindigest
App
ID1
4767
SCX Method Development and pH: ThestandardoperatingpHrangeforLunaSCXis2.0to7.0.MostSCXmethodsaretypicallyrunbetweenpH2.0and5.0foroptimalperformance.Thisensuresthatnitrogen-containinganalytes,especiallythosewithadjacentconjugatedsystemareprotonated.Runninginhighlyacidic(pH<2.0)orbasic(pH>7.0)mobilephasesmaycausethisphasetoundergodegradation,asiscommonforallsilica-basedSCXphases.
see p. 379 for testing probes.2.0-7.03µmand10µmBenzeneSulfonicAcid,StrongCationExchangeAmineandpolyaminecontainingcompoundsGuaranteedtoprovidesharperpeakshapeandbetterresolutioncomparedtotraditionalSCXcolumns
Excellent reproducibilityIt is tough to meet Luna quality standards. The Silica Reproducibility and Bonded Phase Reproducibility Tanaka plots below illustrate the reproducibility of 13 different quality control parameters for 9 batches of Luna material - nearly no variation is observed. Each batch represents hours of quality control testing for over 25 different parameters. We know chromatographers rely on Luna for rugged, reproducible methods worldwide; therefore, an extensive Materials Validation Document (MVD) accompanying each column confirms consistent column specifications and consistent performance.
Material Validation documentQuality Proven
Extensive testing and quality control test data is shown on the Materials Validation Document (MVD) that accompanies each 3 and 5 μm Luna column. Over 25 individual chemical and physical tests are performed and documented on the MVD, including:
Bonding reproducibility test data from 9 Luna 5 μm C18(2) batches. Since differences in bonded phase coverage can show selectivity changes, each Luna batch undergoes 6 tests for bonding.
Silica reproducibility
Quality control test data designed to monitor subtle changes in Luna silica that may affect reproducibility.
Batch No.
0.00
0.50
1.00
1.50
2.00
2.50
3.00
3.50
4.00
4.50
5.00
Hydrogen Bonding
Hydrophobic Index
Metal Test
Inertness
Luna 10 µm C18(2) Batch Number
Luna
10
µm C
18(2
) Sp
eci�
catio
n R
ange
s
5293-1
5293-3
5293-5
5293-7
5293-9
5293-11
5293-13
5293-15
5293-17
5293-19
5293-21
5293-23
5293-25
5293-27
5293-29
5293-31
5293-33
5293-35
5293-37
5293-395293-40
With over 10 years of proven reproducibility, you can be confident in your choice to develop methods on Luna. The following graph shows consistency in both inertness and hydrophobicity across 40 batches of Luna 10 µm C18(2).
171Phenomenex
HPLCLuna
TM
Luna®
MercuryMS™ LC/MS Cartridges (mm)
10 x 2.0 10 x 4.0 20 x 2.0 20 x 4.0Qty Pricing 3 μm Phase I I I I LUNA C18(2) 00N-4251-B0-CE 00N-4251-D0-CE 00M-4251-B0-CE 00M-4251-D0-CE
LUNA C8(2) 00N-4248-B0-CE 00N-4248-D0-CE 00M-4248-B0-CE 00M-4248-D0-CE
3 μm Analytical Columns (mm) SecurityGuard™Cartridges 30 x 3.0 50 x 3.0 150 x 3.0 30 x 4.6 50 x 4.6 75 x 4.6 100 x 4.6 150 x 4.6 4 x 2.0 mm 4 x 3.0 mm Phases
Silica(2) I — I — I 00F-4162-Y0 I — I 00B-4162-E0 I — I 00D-4162-E0 I 00F-4162-E0 AJ0-4347 I AJ0-4348
2.5 μm High Speed Technology (HST) Columns (mm) 30 x 2.0 50 x 2.0 100 x 2.0 50 x 3.0 100 x 3.0 Phase
Luna2.5µmC18(2)-HST I 00A-4446-B0 I 00B-4446-B0 I 00D-4446-B0 I 00B-4446-Y0 I 00D-4446-Y0
Seep.277forinformationaboutHSTColumns.
If Luna does not provide at least anequivalentseparationascomparedtoacompeting columnof the sameparticlesize,similarphaseanddimensions,sendinyourcomparativedatawithin45daysandkeeptheLunacolumnforFREE.
5 μm Analytical, Semi-Prep and Preparative Columns (mm) SecurityGuard™ Cartridges 100 x 4.6 150 x 4.6 250 x 4.6 250 x 10 250 x 15 250 x 21.2 250 x 30 4 x 3.0 mm 10 x 10 mm 15 x 21.2 mm Phases
Silica(2) I 00D-4274-E0 I 00F-4274-E0 I 00G-4274-E0 I 00G-4274-N0I — I 00G-4274-P0 I 00G-4274-U0 AJ0-4348 I AJ0-7223 I AJ0-7229
10 μm Semi-Prep and Preparative Columns (mm) Guards SecurityGuard™Cartridges 250 x 4.6 250 x 10 250 x 15 250 x 21.2 250 x 30 250 x 50 50 x 50 10 x 10 mm 15 x 21.2 mm Phases
Silica(2) I 00G-4091-E0 I 00G-4091-N0 I — I 00G-4091-P0 I — I 00G-4091-V0 03B-4091-V0 AJO-7223 I AJO-7229
*SecurityGuard™ Analytical Cartridges require holder, Part No.: KJ0-4282‡Semi-prep SecurityGuard™ Cartridges require holder, Part No.: AJ0-7220
**PREP SecurityGuard™ Cartridges require holder, Part No.: AJ0-8223
/10pk /3pk /ea
/3pk /ea
174 Phenomenex
HPLC
Luna
TM
Luna®
MEthOd dEVELOPMEnt KitSLuna Method Development Kits combine the excellent performance of Luna HPLC columns with either 3 or 5 different selectivities. The column kits include a Luna Phenyl-Hexyl (moderate polarity) and your choice of a Luna CN (polar) or a C8(2) or C18(2) (non-polars).
MEthOd VALidAtiOn KitSThree columns of the same phase from three different batches provide an excellent benchmark for method validation. Method reproducibility is easily and quickly assessed, clearly demonstrating chromatographic performance validation potential and long term compliance with Luna Method Validation Kits.
OrdEring infOrMAtiOn
Method Development KitsPart no. Description Dimensions (mm) unit Price
KH0-4760 I LunaMethodDevelopmentKit(5columns) I
I I 1eachof5μmC18(2),C8(2),C5,CN,Phenyl-Hexylphases 150x4.6 5/pk
If Luna does not provide at least anequivalentseparationascomparedtoacompeting columnof the sameparticlesize,similarphaseanddimensions,sendinyourcomparativedatawithin45daysandkeeptheLunacolumnforFREE.