GC CARE - High-performance scientific instruments and ... · GC CARE t 4. Bruker’s ... Apart from the inert steel SimDist columns, Bruker offers a ... Low Bleed, High Temperature

Innovation with IntegrityGas Chromatography

Gas Chromatography Columns

GC CARE

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Bruker’s commitment to providing the very best in analytical systems extends beyond providing great instrumentation. In most cases, a good analytical system encompasses instruments, software, and high value consumables. Bruker applies the same demanding standards of performance, utility, and readily available expert support to all components of our analytical systems.

A Selection of GC Columns to Meet Your Needs

Bruker GC columns span a broad range of column diameters, stationary phases, and capillary column materials: Fused Silica (FS) and Inert Steel (IS). Ideal for either routine or research type analyses.

Bruker GC column offerings bridge across many important applications and include a number of offerings such as:

■ Standard WCOT (Wall Coated Open Tubular) Columns with an internal dia-meter ranging from 0.25 mm to 0.53 mm in a variety of stationary phases (from the non-polar BR-1 and BR-1ms phase up to the wax phases) for narrow and wide analyte concentration ranges.

■ Small Internal Diameter Columns for fast analysis that improve cycle time while maintaining high resolution.

■ Solid Stationary Phase PLOT (Porous Layer Open Tubular) Columns for the analysis of volatiles that require high selectivity. The solid stationary phase is tuned for applications commonly used for the permanent gas analysis like the BR-Molsieve 5A and petro-chemical analysis like the BR-Alumina range.

■ Inert Steel Micro-Packed and Packed Columns for composition analysis with bulk components, where a high column capacity is required.

Petrochemical and Hydrocarbon Analysis

Bruker provides the most comprehensive range of GC analysis solutions for ana-lysts working in the Petrochemical indu-stry. Bruker configures, produces, tests, delivers, and installs gas chromatogra-phy hardware, complete with columns, software and consumables according to widely used industry standards (e.g. ASTM, UOP, ISO, GPA, EN). This saves you time and ensures confidence in your results.

Directed Solutions for Distillation

Simulated distillation per ASTM D7169 is used for determining the boiling point range distribution of petroleum products by capillary gas chromatography of heavy distillate and residual fuel oils with initial boiling points (IBP) > 100°C and final boiling point (FBP) above 720°C. The sta-tionary phase must meet rigid resolution and retention time requirements, yet be stable at high temperatures.

To meet the critical criteria set forth by ASTM, Bruker is using the BR-1HT SimDist column. The BR-1HT polymer is a 100% polydimethylsiloxane (PDMS) material that requires minimal conditio-ning, and is 100% crosslinked and coated onto highly deactivated stainless steel tubing that has the inertness of fused silica without the temperature limitations.The BR-1HT inert steel SimDist column has a lifetime of at least 400 injections under typical SimDist conditions.

Figure 1 shows a Polywax 1000 separation on a BR-1HT SimDist column. Chromatogram was obtained under temperature programming up to 430oC.

Additional Petrochemical Applications

Apart from the inert steel SimDist columns, Bruker offers a complete column product range for the petrochemical industry including (micro-)packed for refinery gas analysis, PLOT columns for selective volatiles analysis, high resolution columns for detailed hydrocarbon analysis.

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Low Bleed, High Temperature

High Selectivity Separation

Figure 2 shows excellent retention and selectivity on a BR Alumina Na2SO4 PLOT column for a complex refinery gas sample; complete separation up to Hexanes within 15 minutes.

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1. methane2. ethane3. ethylene4. propane5. propylene6. isobutane7. n-butane8. propadiene9. acetylene10. trans-2-butene11. 1-butene12. isobutylene13. cis-2-butene14. isopentane15. n-pentane16. 1,3-butadiene17. trans-2-pentene18. 2-methyl-2-butene19. 1-pentene20. cis-2-pentene21. hexanes

Pharmaceutical

Gas chromatography in the pharmaceu-tical industry has been identified as a powerful tool to analyze volatiles in complex samples. With today’s more complex pharmaceuticals/biotherapeu-tics and sophisticated formulations and delivery systems, the need for precise and accurate analysis to deliver safe and effective drugs has become extremely urgent.

Head Space Testing and Residual Solvent Analysis

The United States Pharmacopeia (USP) incorporated headspace-GC analysis into its Residual Solvents Chapter: USP 467. Although analysis of the headspace matrix is usually straightforward, Bruker offers the columns to separate and analyze these components in a fast and accurate way. Due to the nature of some solvents like small organic amines, high column inertness is required to generate a symmetrical peak shape and as a result improve identification and quantitation especially at lower concentrations.

Analysis of Polar Unknowns

For confirmation analysis of polar unknowns, a GC/MS configuration is used. To optimize speed of analysis, and enable an optimal selectivity while minimizing column bleed the Bruker ‘ms’ column line offers the most versatile solutions. For example the BR-624ms is a medium polar and provides improved retention and selectivity for also polar compounds and are also more compa-tible with polar injection solvents.

High column inertness is essential for polar component analysis. With the Bruker inert columns, highly polar com-ponents like glycols, and sulfonates like mesylate, besylate, and tosylate that are sensitive to react with the column phase, give the peak symmetry needed for good quantification.

Figure 1: Improve system suitability pass rates with greater resolution on BR-624ms columns

Greater Resolution

Higher Sensitivity

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1. Methanol2. Acetonitrile3. Dichloromethane4. trans-1,2-Dichloroethene5. cis-1,2-Dichloroethene6. Tetrahydrofuran7. Cyclohexane8. Methylcyclohexane9. 1,4-Dioxane10. Toluene11. Chlorobenzene12. Ethylbenzene13. m-Xylene14. p-Xylene15. o-Xylene

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1. Methanol2. Acetonitrile3. Dichloromethane4. trans-1,2-Dichloroethene5. cis-1,2-Dichloroethene6. Tetrahydrofuran7. Cyclohexane8. Methylcyclohexane9. 1,4-Dioxane10. Toluene11. Chlorobenzene12. Ethylbenzene13. m-Xylene14. p-Xylene15. o-Xylene

1. Methyl methanesulfonate (MMS) 2. Isopropyl methanesulfonate (iPMS) 3. Diethyl sulfate (DES) 4. di-isopropyl sulfate (DPS) 5. dibutyl sulfate (DBS) 6. Methyl benzenesulfonate (MBS) 7. Ethyl benzenesulfonate (EBS) 8. Methyl toluenesulfonate (MTS) 9. Ethyl toluenesulfonate (ETS) 10. n-propyl toluenesulfonate (nPTS) 11. n-butyl benzenesulfonate (nBBS) 12. isopropyl p-toluenesulfonate (iPTS) 13. p-toluenesulfonic acid n-butyl ester (nBTS)

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1. Methyl methanesulfonate (MMS) 2. Isopropyl methanesulfonate (iPMS) 3. Diethyl sulfate (DES) 4. di-isopropyl sulfate (DPS) 5. dibutyl sulfate (DBS) 6. Methyl benzenesulfonate (MBS) 7. Ethyl benzenesulfonate (EBS) 8. Methyl toluenesulfonate (MTS) 9. Ethyl toluenesulfonate (ETS) 10. n-propyl toluenesulfonate (nPTS) 11. n-butyl benzenesulfonate (nBBS) 12. isopropyl p-toluenesulfonate (iPTS) 13. p-toluenesulfonic acid n-butyl ester (nBTS)

Figure 2: The BR-624ms phase is more compatible with polar injection solvents than type BR-1 or BR-5 columns, providing higher sensitivity and less time needed for optimizing injection parameters. Multiple traces using MS detection.

Governed by institutions as EPA and EN, today’s Environmental analysis market is more stringent and challenging than ever. The levels of active or hazardous com-ponents to be measured are extremely low, whilst the array of sample matrices encountered is wide and most often quite challenging.

Bruker Capillary Columns offer exceptio-nal thermal stability in combination with extremely low bleed and high inertness levels, to give you the optimum perfor-mance for your GC or GC/MS system. All these column features help you meet the analysis quality requirements set forth by various regulatory agencies. Analyte Class:■ Semi volatiles■ PCB’s & Arochlors■ Pesticides ■ Volatiles■ Polyaromatic Hydrocarbons

Environmental Analysis

Exceptional column quality minimizes downtime and optimizes profit per sample, while fully maintaining the inte-grity of your results. Bruker offers a com-plete package of solutions, with columns engineered and produced precisely for the application you need.

Optimization for the Analysis of Volatiles and Pesticides

An example of application-based column optimization is our solution for EPA Method 8270 semi-volatiles. The BR-5MS provides excellent column inertness towards acidic, basic, and neutral compounds found in this analy-ses. BR-5MS also provides excellent resolution of high molecular weight PAHs like benzo (b) and (k) fluoranthenea and possess unsurpassed thermal stability make this column the best choice for the method.

Figure 1: shows illustration of method EPA 8270 on a BR-5ms column

Method EPA 8270

1. 1,4-Dioxane

2. N-Nitrosodimethylamine

3. Pyridine

51. 2,4-Dinitrophenol

87. Benzo[b]fluoranthene

88. Benzo[k]fluoranthene

Clinical and Forensic Analysis

Analysis of post-mortem tissue or biofluid samples is challenged by producing criti-cal evidence that stands up under severe scrutiny. Pressure for fast & definitive results drives labs to explore potential to improve quality of data or speed methods up without compromising data quality.

Drug Screening and Forensic Analysis

Bruker provides you with the best solu-tions in the Clinical/Forensic workplace with columns that are manufactured and individually tested to meet stringent requirements for inertness, low bleed, and unsurpassed column to column reproducibility.

The exceptional inertness improves peak shape over ‘normal’ 5% diphenyl-phase columns. As a result, analytical sensitivity for medium-polar active components such as derivatized amphetamines or cocaine improves significantly. This ruggedness also ‘lasts longer’, even after repeated injections with aggressive derivatization reagents. Utilizing the same column derivatized cannabinoids and a wide range of other regular drugs may be analyzed with similar or better results.

Figure 1: shows the analysis of derivated amphetamines. The rugged column results in a highly stable performance on the BR-5ms, even under the most demanding analytical conditions and its excpetional inertness ensure good peak shapes for reproducible quantitation.

Figure 2 shows the analysis of derivatized cannabinoids. Again, the stability of the BR-5ms results in exceptional performance. This column can be used on a wide range of other regular drugs, such as opiates or cocaine with similar performance.

Exceptional Performance

Wide Range of Use

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1. Amphetamine 2. Methamphetamine 3. MDA 4. MDMA 5. MDEA c = contaminant

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1. Cannabidiol2. delta-9-THC3. Cannabinol4. THC-COOH; THCA

There is an immense range of analytes of interest and matrices that can be encoun-tered in the analysis of food, flavor, and fragrances. Many governing organiza-tions have developed requirements for testing these important products. Recent developments and product quality issues have driven the need for more and more content product labeling and reporting.

Analysis of Fatty Acids and Oils in Foods

For Food applications, Bruker offers a range of gas chromatographic columns for a number of analyses described by organizations like the Association of Official Analytical Chemists (AOAC) Inter-national, AOCS (American Oil Chemists Society), AACC (American Association of Cereal Chemists) and INA (Insti tute for Nutraceutical Advancement).

Bruker offers solutions for the analysis of Fats & Oils for free fatty acids content either directly for volatile acids or methyl derivatized for non- and semi-volatiles (FAMES). For triglycerides analysis Bruker offers columns that are capable of operate at elevated column temperature conditions. Also for cholesterol and other dietary Sterols column solutions are available.

Analysis of Composition in Flavors and Fragrances

Complex samples like flavors and fragran-ces require powerful analytical techni-ques to separate components of interest. Besides the high separation power also, by optimizing the polarity of the statio-nary phase, the Bruker GC columns have additional selectivity that allows better identification and quantification of many key components of these naturally occur-ring and synthetic products.

Food, Flavors and Fragrances

High Temperature Application

1. 5-a-cholestane (IS) 2. Cholesterol

Figure 1: chromatography of underivatized cholesterol, column programmed to 330°C.

Powerful Separation

Figure 2: split injection of a neat lemon oil on the BR-5 column

Powerful Separation

1. heptanol2. a-thujene3. a-pinene4. camphene5. sabinene6. β-pinene7. 6-methyl-5-hepten-2-one8. myrcene9. octanal

10. a-phellandrene11. 3-carene12. a-terpinene13. p-cymene14. limonene15. a-terpinene16. octanol17. terpinolene18. linalool19. nonanal

20. citronellal21. terpinene-4-ol22. a-terpineol23. decanol24. octyl acetate25. nerol26. neral27. carvone28. geraniol29. geranial

30. nonyl acetate31. citronellyl acetate32. neryl acetate33. geranyl acetate34. dodecanal35. β-caryophyllene36. trans-a-bergamotene37. a-humulene38. β-bisabolene

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Bruker Daltonik GmbH

Bremen · GermanyPhone +49 (421) 2205-0 Fax +49 (421) 2205-103 [email protected]

Bruker Daltonics Inc.

Billerica, MA · USAPhone +1 (978) 663-3660 Fax +1 (978) 667-5993 [email protected]

www.bruker.com/chemicalanalysis

A Complete Range of High Performing Stationary Phases

Bruker Phase Composition Similar Stationary Phases

BR-1ms 100% dimethyl polysiloxane HP-1ms UI, DB-1ms UI, HP-1, HP-1ms, DB-1,DB-1ms, Ultra-, 1 VF-1ms, CP-Sil 5,

CP Sil 5 CB Low Bleed/MS, Rxi-1ms, ZB-1, ZB-1ms, BP-1, Optima-1, Optima-1ms, SPB-1, Equity-1

BR-1HT 100% dimethyl polysiloxane DB-1HT , Rxi-1HT , ZB-1HT

BR-5 5% diphenyl HP-5ms UI, HP-5, HP-5ms, DB-5, Ultra-2, 95% dimethyl polysiloxane CP-Sil 8, CP Sil 8 CB, Rxi-5ms, ZB-5, BP-5,

Optima-5, SPB-5, Equity-5

BR-5ms 5% phenyl DB-5ms UI, DB-5ms, VF-5ms, CP-Sil 8 CB 95% dimethyl arylene siloxane Low Bleed/MS, Rxi-5Sil MS, ZB-5MS,

BPX-5, Optima-5ms, SLB-5

BR-5HT 5% diphenyl DB-5HT, VF-5HT, Rxi-5HT, ZB-5HT 95% dimethyl polysiloxane

BR-XLB arylene/ DB-XLB, VF-Xms, Rxi-XLB methyl modified polysiloxane

BR-624ms 6% cyanopropyl phenyl DB-624, HP-624, VF-624ms, Rxi-624Sil MS, 94% dimethyl arylene siloxane ZB-624, BP-624, Optima-624

BR-35ms 35% phenyl DB-35ms, VF-35ms, Rxi-35, Sil MS, MR2 65% dimethyl arylene siloxane

BR-17 50% diphenyl HP-17, DB-17, DB-608 ,CP-Sil 24 CB, Rxi-17, 50% dimethyl polysiloxane ZB-50

BR-17ms 50% phenyl DB-17ms, VF-17ms, Rxi-17Sil MS, BPX-50 50% dimethyl arylene siloxane

BR-Alumina Alumina Na2SO4 deactivated Rt-Alumina Bond, GS-Alumina, Na2SO4 HP PLOT S, CP-Al2O3/Na2SO4, Alumina-PLOT, AT-Alumina

BR-Q PLOT Divinylbenzene polymer RT-Q-BOND, CP-PoraPLOT Q, CP-PoraBond Q, Supel-Q-PLOT, AT-Q

BR-QS PLOT Porous divinylbenzene polymer RT-QS-BOND, GS-Q

BR-S PLOT Divinylbenzene 4-vinylpyridine RT-S-BOND, CP-PoraPLOT S, Supel-G45

BR-U PLOT Divinylbenzene ethylene HP-PLOT U, RT-U-BOND, CP-PoraPLOT U, glycol/dimethylacrylate CP-PoraBond U, Supel-N PLOT

BR-Molsieve5A Molecular Sieve 5A RT-Molsieve 5A, GS-Molsieve, HP PLOT Molsieve, CP-Molsieve 5A, AT-Molsieve, PLT-5A