Flow Particle Image AnalyzerFPIA-3000/FPIA-3000S Operator’s Manual CHAPTER 1: FOR YOUR SAFETY CHAPTER 2: EXTERNAL APPEARANCE AND FU NCTI ONS CHAPTER 3: PREPARATIONS F OR T ESTING CHAPTER 4: TESTING CHAPTER 5: RECORD DISPLAY AND PROCESSING CHAPTER 6: QUALITY CONTROL CHAPTER 7: TROUBLESHOOTING CHAPTER 8: SOP SETTINGS CHAPTER 9: SYSTEM SETUP CHAPTER 10 ANALYSIS PARAMETERS CHAPTER 11 DISPLAY SETUP CHAPTER 12 PRINT FORMAT CHAPTER 13 USER AUTHENTICATION CHAPTER 1 4 SYSTEM ADJUSTMENT CHAPTER 1 5 MAINTENANCE CHAPTER 16 FUNCTIONAL DESCRIPTION APPENDIX A. INSTALLATION APPENDIX B. SPECIFICATION AND PERFORMANCE OF THE FPIA-3000 /3000 S APPENDIX C. TABLE OF SYSTEM SETTINGS APPENDIX D. OUTPUT FORMAT APPENDIX E. GAS SENSOR SETTING VALUES AND LIST OF FPIA-3000S RECOGNIZED ORGANIC SOLVENTS APPENDIX F. FOCUS ADJUSTING SAMPLE APPENDIX G. ULTRASONIC DISPERSION UNIT MANUAL ( OPTI ONAL) APPENDIX H. HIGH/LOW MAGNIFICATION LENS UNIT MANUAL ( OPTI ON) APPENDIX I. PART11 SOFT WARE MANUAL (OPTI ONAL ) APPENDIX J. FPIA-3000 DARK FIELD UNIT MAN UAL SYSMEX CORPORATION KOBE, JAPAN Copyright ® 2004-2006 by SYSMEX CORPORATION All rights reserved. No part of this Operator’s Manual may be Code No. 461-2139-2 reproduced in any form or by any means whatsoever without PRINTED IN JAPAN prior written permission of SYSMEX CORPORATION. Date of Last Revision: August 2006
This document is posted to help you gain knowledge. Please leave a comment to let me know what you think about it! Share it to your friends and learn new things together.
Transcript
7/18/2019 FPIA3000 User Manual English August 2006
CHAPTER 1: FOR YOUR SAFETYCHAPTER 2: EXTERNAL APPEARANCE AND FUNCTIONSCHAPTER 3: PREPARATIONS FOR TESTING
CHAPTER 4: TESTINGCHAPTER 5: RECORD DISPLAY AND PROCESSING
CHAPTER 6: QUALITY CONTROLCHAPTER 7: TROUBLESHOOTINGCHAPTER 8: SOP SETTINGSCHAPTER 9: SYSTEM SETUPCHAPTER 10 ANALYSIS PARAMETERSCHAPTER 11 DISPLAY SETUPCHAPTER 12 PRINT FORMATCHAPTER 13 USER AUTHENTICATIONCHAPTER 14 SYSTEM ADJUSTMENT
CHAPTER 15 MAINTENANCECHAPTER 16 FUNCTIONAL DESCRIPTIONAPPENDIX A. INSTALLATIONAPPENDIX B. SPECIFICATION AND PERFORMANCE OF THE FPIA-3000/3000SAPPENDIX C. TABLE OF SYSTEM SETTINGSAPPENDIX D. OUTPUT FORMATAPPENDIX E. GAS SENSOR SETTING VALUES AND LIST OF FPIA-3000S
RECOGNIZED ORGANIC SOLVENTS
APPENDIX F. FOCUS ADJUSTING SAMPLEAPPENDIX G. ULTRASONIC DISPERSION UNIT MANUAL (OPTIONAL)APPENDIX H. HIGH/LOW MAGNIFICATION LENS UNIT MANUAL (OPTION)APPENDIX I. PART11 SOFTWARE MANUAL (OPTIONAL)APPENDIX J. FPIA-3000 DARK FIELD UNIT MANUAL
SYSMEX CORPORATIONKOBE, JAPAN
Copyright® 2004-2006 by SYSMEX CORPORATION
All rights reserved. No part of this Operator’s Manual may be Code No. 461-2139-2reproduced in any form or by any means whatsoever without PRINTED IN JAPANprior written permission of SYSMEX CORPORATION. Date of Last Revision: August 2006
7/18/2019 FPIA3000 User Manual English August 2006
Sysmex FPIA-3000/FPIA-3000S Operator’s Manual -- Revised December 2005
Sysmex is a registered trademark of Sysmex Corporation.
• PARTICLE SHEATH is a registered trademark of Sysmex Corporation.
• Windows is a registered trademark of Microsoft Corporation.
• All other company names and product names contained in this document may be trademarks or
registered trademarks of the respective owners.
• This document may not be copied or published in part or in its entirety without permission.
• Some of the screens that appear in this document may be different from what you mayactually see on your screen.
• Because of ongoing improvements made to the product, some of the descriptions containedin this document may not apply to the specific product you are using.
7/18/2019 FPIA3000 User Manual English August 2006
Chapter 2 EXTERNAL APPEARANCE AND FUNCTIONS1. Overview of the device.................................................................................2-12. System configuration ...................................................................................2-23. Overview of screen displays and switches ..................................................2-3
3.1 Names of components of the testing unit ..............................................2-33.2 Screen display of the data processing unit............................................2-4
4. Emergency stop...........................................................................................2-65. Types of alarms ...........................................................................................2-66. Packaging ....................................................................................................2-77. Installation....................................................................................................2-7
7.1 Installing and moving the instrument .....................................................2-77.2 Installation location................................................................................2-77.3 Grounding..............................................................................................2-8
8. Reagents......................................................................................................2-89. Names and functions of the components of the FPIA-3000/3000S...........2-11
9.1 Front panel/right front panel.................................................................2-119.2 Rear side of the testing unit .................................................................2-13
9.3 Pneumatic unit .....................................................................................2-1510. Disposal ...................................................................................................2-17
Chapter 3 PREPARATIONS FOR TESTING1. Items to be checked before turning on the device .......................................3-1
1.1 Reagents ...............................................................................................3-11.2 Printer paper (when connected to a printer) ..........................................3-11.3 Instrument inspection.............................................................................3-1
2. Turning on the device ..................................................................................3-23. Starting the analysis program and background check.................................3-3
3.1 Starting the analysis program................................................................3-33.2 Sheath liquid replacement .....................................................................3-53.3 Background Check ................................................................................3-73.4 Auto Focusing........................................................................................3-9
Chapter 4 TESTING1. Test flow.......................................................................................................4-12. Check instrument status ..............................................................................4-23. Test information setting................................................................................4-4
7/18/2019 FPIA3000 User Manual English August 2006
4. Test procedure ..........................................................................................4-114.1 Preparing a sample .............................................................................4-114.2 Testing a sample................................................................................. 4-12
5. Display of analysis results ......................................................................... 4-146. Shutting down (turning off the power) .......................................................4-15
Chapter 5 RECORD DISPLAY AND PROCESSING1. Record Display and Processing ..................................................................5-1
1.1 Processing the record list...................................................................... 5-11.2 Screen composition............................................................................... 5-11.3 Explanation of functions ........................................................................ 5-2
1.4 Sort functions ........................................................................................ 5-71.5 The filter function...................................................................................5-91.6 Search functions .................................................................................5-111.7 Display reset .......................................................................................5-131.8 Sample information revision................................................................ 5-141.9 Reanalyze ...........................................................................................5-151.10 Deletion.............................................................................................5-161.11 Merging records ................................................................................5-161.12 Copying to the clipboard ................................................................... 5-171.13 Changing the record display ............................................................. 5-17
2.1 Introduction .........................................................................................5-182.2 Screen composition.............................................................................5-182.3 Moving records....................................................................................5-202.4 Thumbnail image display .................................................................... 5-212.5 Reanalyze ...........................................................................................5-222.6 Selection particle save ........................................................................ 5-342.7 Changing the display of the Analysis results screen........................... 5-34
3. Particle image list ...................................................................................... 5-353.1 Introduction .........................................................................................5-353.2 Screen composition.............................................................................5-353.3 Copying to the clipboard .....................................................................5-36
3.4 Detailed information display ................................................................5-374. Frequency tables....................................................................................... 5-38
4.1 Introduction .........................................................................................5-384.2 Screen composition.............................................................................5-384.3 Copying to the clipboard .....................................................................5-38
5. Multi Scattergrams ....................................................................................5-395.1 Introduction .........................................................................................5-395.2 Screen composition.............................................................................5-39
7/18/2019 FPIA3000 User Manual English August 2006
6. Graph (Overlay-View) ................................................................................5-406.1 Introduction..........................................................................................5-406.2 Screen composition .............................................................................5-416.3 Copying to the clipboard......................................................................5-416.4 Setting up display ................................................................................5-41
7. Meta Data ..................................................................................................5-427.1 Introduction..........................................................................................5-427.2 Screen composition .............................................................................5-427.3 Copying to the clipboard......................................................................5-42
8. Detail Results.............................................................................................5-438.1 Introduction..........................................................................................5-438.2 Screen composition .............................................................................5-438.3 Copying to the clipboard......................................................................5-43
9. Graph (Trend-View) ...................................................................................5-449.1 Introduction..........................................................................................5-449.2 Screen composition .............................................................................5-449.3 Copying to the clipboard......................................................................5-459.4 Setting up display ................................................................................5-45
10. The File menu..........................................................................................5-4510.1 File menu items .................................................................................5-4510.2 New ...................................................................................................5-4610.3 Open..................................................................................................5-4710.4 Import ................................................................................................5-48
10.5 Save ..................................................................................................5-5010.6 Printing ..............................................................................................5-5210.7 PDF Print ...........................................................................................5-5310.8 CSV export ........................................................................................5-5510.9 Transmission to the host....................................................................5-56
Chapter 6 QUALITY CONTROL1. Quality control ..............................................................................................6-1
1.1 Overview of quality control.....................................................................6-11.2 File Settings...........................................................................................6-21.3 Adding a quality control file....................................................................6-3
1.4 Deleting a quality control file..................................................................6-51.5 Target setting.........................................................................................6-61.6 Auto target .............................................................................................6-61.7 Deleting a quality control data ...............................................................6-7
IV Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006
1.2 Reverting from an error ......................................................................... 7-21.3 Display of error history .......................................................................... 7-3
2. Error message list .......................................................................................7-42.1 Error message (in alphabetical order)................................................... 7-42.2 Error code list........................................................................................ 7-62.3 Error message list by function............................................................... 7-7
3. Error messages and troubleshooting ..........................................................7-9
1.1 Explanation of SOP...............................................................................8-11.2 Using SOPs........................................................................................... 8-11.3 Registering SOPs.................................................................................. 8-11.4 Changing SOP's name.......................................................................... 8-21.5 Editing and deleting SOPs ....................................................................8-21.6 SOP Import / Export ..............................................................................8-4
Chapter 9 SYSTEM SETUP1. System setup............................................................................................... 9-1
1.1 Setting menu items ............................................................................... 9-11.2 System settings.....................................................................................9-1
2. Sheath fluid settings (Only for the FPIA-3000S) ......................................... 9-52.1 Effects by sheath physical characteristics............................................. 9-52.2 Sheath fluid specific hardware settings................................................. 9-6
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006 V
Chapter 13 USER AUTHENTICATION1. User Authentication....................................................................................13-1
1.1 Change password................................................................................13-11.2 User Registration.................................................................................13-21.3 Lockout ..............................................................................................13-14
1.4 Setting Default Database...................................................................13-141.5 Change logon user ............................................................................13-15
Chapter 14 SYSTEM ADJUSTMENT1. Adjusting the pressure of the pneumatic unit.............................................14-1
1.1 Adjusting the pressure.........................................................................14-12. Adjusting the pressure of the testing unit...................................................14-2
2.1 Checking the pressure of the testing unit ............................................14-22.2 Pressure adjustment points .................................................................14-32.3 Adjustment of rinse pressure...............................................................14-42.4 Adjusting the -0.05 MPa Pressure.......................................................14-5
2.1 Auto rinse ............................................................................................15-12.2 Revert from error .................................................................................15-22.3 Manual focusing...................................................................................15-22.4 Background Check ..............................................................................15-42.5 Sheath liquid exchange .......................................................................15-52.6 Compressor ON...................................................................................15-8
2.7 Cover open/close.................................................................................15-82.8 Adjustment of the flash brightness.......................................................15-82.9 Stir motor adjustment...........................................................................15-8
3. Changing fuses ..........................................................................................15-93.1 Changing the fuse in the testing unit ...................................................15-93.2 Changing the fuse in the pneumatic unit ...........................................15-103.3 Periodic Replacement Parts ..............................................................15-11
4. Help..........................................................................................................15-114.1 Table of Contents ..............................................................................15-114.2 Version information............................................................................15-11
Chapter 16 FUNCTIONAL DESCRIPTION1. Testing principles .......................................................................................16-12. Image processing.......................................................................................16-23. Particle analysis .........................................................................................16-34. Statistical analysis......................................................................................16-55. List of display items....................................................................................16-7
5.1 Particle diameter parameters...............................................................16-75.2 Form parameters .................................................................................16-8
7/18/2019 FPIA3000 User Manual English August 2006
VI Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006
5.3 Other test data ....................................................................................16-95.4 Statistical analysis items .....................................................................16-95.5 Analysis data.....................................................................................16-10
Appendix A INSTALLATION
1. Preparation for installation...........................................................................A-11.1 Accessories...........................................................................................A-11.2 Place and space for installation ............................................................A-51.3 Exterior dimensions...............................................................................A-7
2. Installation ...................................................................................................A-82.1 Dismantling the fastener for transportation ...........................................A-82.2 Mounting the Filter Assembly No. 24 (FPIA-3000S) ...........................A-152.3 Connecting the tubes ..........................................................................A-172.4 Connecting the power cables and connection cables .........................A-222.5 Grounding ...........................................................................................A-252.6 Installing the analysis PC ....................................................................A-26
3. Installation of Acrobat Reader ...................................................................A-30
Appendix B SPECIFICATION AND PERFORMANCE OF THE FPIA-3000/
Appendix C TABLE OF SYSTEM SETTINGS1. Table of system settings............................................................................. C-12. Table of SOP settings ................................................................................ C-3
Appendix D OUTPUT FORMAT1. Summary.................................................................................................... D-12. Serial communications ............................................................................... D-1
2.1 Hardware.............................................................................................. D-12.2 Communication Format........................................................................ D-12.3 Signal Level.......................................................................................... D-22.4 Protocols used in communication with the host ................................... D-22.5 Class A communications...................................................................... D-22.6 Class B communications...................................................................... D-32.7 Processing of communication errors with the host............................... D-4
2.8 Monitoring the receiver (host) side....................................................... D-43. Ethernet communications........................................................................... D-53.1 Hardware.............................................................................................. D-53.2 Software............................................................................................... D-53.3 Text format ........................................................................................... D-63.4 Transmission format selection ............................................................. D-6
4. Data format................................................................................................. D-74.1 Test results (limited data)..................................................................... D-74.2 Histogram data..................................................................................... D-9
7/18/2019 FPIA3000 User Manual English August 2006
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006 VII
4.3 Scattergram data ................................................................................D-114.4 Extend data ........................................................................................D-13
Appendix E GAS SENSOR SETTING VALUES AND LIST OF FPIA-3000S
RECOGNIZED ORGANIC SOLVENTS1. Gas sensor level setting values ..................................................................E-12. List of FPIA-3000S recognized organic solvents ........................................E-2
Appendix F FOCUS ADJUSTING SAMPLE1. Focus adjusting particle .............................................................................. F-12. Preparation of focus adjustment sample..................................................... F-2
Appendix G ULTRASONIC DISPERSION UNIT MANUAL (OPTIONAL)1. FPIA-3000 Optional Unit (Ultrasonic Dispersion Unit) ................................G-1
1.1 Overview...............................................................................................G-12. Settings and Usage.....................................................................................G-2
3. Background Count Generated by Erosion ..................................................G-53.1 When using the Standard Magnification Unit........................................G-53.2 When using the High Magnification Unit...............................................G-63.3 When using the Low Magnification Unit................................................G-7
4. Sample Temperature Arisen when Ultrasonic Scanned .............................G-7
Appendix H HIGH/LOW MAGNIFICATION LENS UNIT MANUAL (OPTION)1. FPIA-3000 Optional Lens Unit ....................................................................H-1
1.1 Features ...............................................................................................H-11.2 General Information of the Lens Unit....................................................H-2
2. Installation...................................................................................................H-32.1 Preparation for installation....................................................................H-32.2 Installation (Changing hardware)..........................................................H-4
3. Preparation for testing...............................................................................H-113.1 Starting the analysis program.............................................................H-11
Appendix I PART11 SOFTWARE MANUAL (OPTIONAL)1. FPIA-3000 Optional Unit (Software Conforming to Part 11) .........................I-1
1.1 Features .................................................................................................I-12. Installation of the Part 11 Software...............................................................I-2
7/18/2019 FPIA3000 User Manual English August 2006
VIII Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006
2.1 Install...................................................................................................... I-23. Using the FPIA-3000 Part 11 Software ........................................................ I-3
3.1 Preparation for testing............................................................................ I-33.2 Testing ................................................................................................... I-33.3 Stored Data Display and Processing ..................................................... I-33.4 Error Treatment...................................................................................... I-33.5 SOP settings .......................................................................................... I-33.6 User Verification..................................................................................... I-33.7 Audit Trail Function ................................................................................ I-43.8 Others .................................................................................................... I-4
Appendix J FPIA-3000 DARK FIELD UNIT MANUAL1. Overview ..................................................................................................... J-1
1.1 Overview............................................................................................... J-11.2 Principle of Dark Field Test................................................................... J-1
5.1 Starting the analysis program ............................................................. J-105.2 Replacing the Sheath Fluid ................................................................. J-12
5.3 Process executed after replacing the FPIA-3000 Dark Field Unit....... J-155.4 Checking Light Field System............................................................... J-15
9.1 Quality Control .................................................................................... J-289.2 Adjustment of the flash brightness...................................................... J-28
7/18/2019 FPIA3000 User Manual English August 2006
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006
Chapter 1 FOR YOUR SAFETY
1. How to use this product safely .................................................................... 1-11.1 Explanation of labeling .......................................................................... 1-1
1-2 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005
WARNING
• If you smell something strange or notice fumes, turn off the power switch immediately, and unplug
the power cord.
Failure to do so could result in fire, electrocution or injury.Contact your dealer or the nearest Sysmex office immediately for an inspection.
• Do not spill liquids, such as reagents, and do not put metallic items such as staples or clips inside
the instrument, to avoid short circuits or fumes.
Shorting and smoke emission could result. Should any abnormality occur, turn off the instrumentimmediately, unplug the power cord the outlet, and contact your dealer or the Sysmex office for an
inspection.
• Never open the instrument cover or case while the instrument is running.
Doing so may result in electric shock or injury.
• Be sure to wear rubber gloves during maintenance or inspection.
Use the specified tools and components.After finishing work, wash your hands with disinfectant.
• Pay special attention when handling waste liquid.
If your body or clothes come in contact with waste water, wash it off with disinfectant.
Handling of organic solvents
• Do not use fire when you use inflammable organic solvents.
It may cause fire.
• Ventilate the instrument circumference.
It may cause a fire or exposure to toxic substance.
• Please install the fire extinguisher corresponding to the organic solvent near the instrument.
• When you preparing a sample, exchange sheath liquid or waste liquid, please use protection
implements (e.g. glove, mask, glasses) according to the MSDS.
• If you swallow a reagent or get it in your eyes or on your skin, take first-aid action according to the
MSDS, and call a doctor immediately.
• When you use an organic solvent, please take necessary measures according to MSDS.
7/18/2019 FPIA3000 User Manual English August 2006
1-4 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006
CAUTION
Use of reagents
• After opening the container, make sure that no dust, dirt or bacteria get in.
• Do not use a reagent that has passed its expiry date.
• Handle reagents carefully so that no bubbles are formed in them.
• Be careful not to spill reagents. If they spill, wipe them off with a wet cloth immediately.
• Follow the directions given on the container.
• If you consider yourself to be inexperienced in using the instrument, be sure to use it under guidance
or with the assistance of an experienced person.
• In the event of any malfunction of the instrument, the administrator of the instrument should first
attempt to solve the problem according to the instructions explicitly described in this manual. If thereare no appropriate instructions in the manual, contact your dealer or the Sysmex office for an
inspection.
• Unpacking, installation and confirmation of the initial operation are to be performed by service
personnel from your dealer or a Sysmex office.
About the environment of operation:
• Install the instrument in a place where there is no risk of it being splashed with water.
• Avoid high temperatures, high humidity, dust or direct sunlight.
• Do not subject the instrument to strong vibrations or shocks.
• Do not install the instrument in the vicinity of chemicals or gas.
Handling of software:
• In the data processing unit, do not install software other than that specified by Sysmex to avoid
abnormal operation of software.
The software might not function normally in some circumstances.
• Exit the FPIA-3000 application before executing any other application.
When connecting peripherals:
• We do not guarantee connectiion availability between printers or peripherals and personal
computers.
Contact any manufacturers of personal computers or peripherals you use.
• Use the built-in hard disk for the data storage folder to store test data obtained using the FPIA-3000.
• Do not use peripheral equipment such as an external hard disk for the database folder to store test
data since the use of such device might prevent the FPIA-3000 from performing its normal test
operation.
7/18/2019 FPIA3000 User Manual English August 2006
1-6 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005
3. Signs
What They Mean
Signs used in the explanations for operating the instrument
Explanations of operation methods in this manual use the following notation:
• A button name within brackets means a button for a data-processing program.
(e.g., [OK], [Cancel] )
• [XYZ] also indicates the name of a menu.
(e.g., [File], [Stored data screen] )
WARNINGThis indicates situations where working while carrying a static chargecould result in explosive ignition by static electricity.
WARNINGMishandling of the instrument by ignoring this note may cause death orsevere injury to the user and/or serious physical damage to property.
CAUTIONMishandling of the instrument by ignoring this note may cause injury to theuser, affect output results or cause physical damage to property.
CAUTION: Items that users should know in order to maintain the performance of andprevent damage to the instrument.
NOTE: Information that is useful to know when using the instrument.
NOTE: • Some of the screens that appear in this document may be different from what
you actually see on your screen or print out.• Because of ongoing improvements made to the product, some of thedescriptions contained in this document may not apply to the specific productyou are using.
7/18/2019 FPIA3000 User Manual English August 2006
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006
Chapter 2 EXTERNAL APPEARANCE AND FUNCTIONS
1. Overview of the device ................................................................................2-1
2. System configuration...................................................................................2-2
3. Overview of screen displays and switches.................................................. 2-33.1 Names of components of the testing unit.............................................. 2-33.2 Screen display of the data processing unit ........................................... 2-4
9. Names and functions of the components of the FPIA-3000/3000S .......... 2-11
9.1 Front panel/right front panel ................................................................ 2-119.2 Rear side of the testing unit ................................................................2-139.3 Pneumatic unit ....................................................................................2-15
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005 2-1
1. Overview of the device
The FPIA-3000 is a Flow Particle Image analyzer combining flat sheath flow formation technology
and image processing technology.
The FPIA-3000 guides a suspension of particles to a transparent flow cell to form a flat sample
flow, and irradiates pulse light to the flat sample flow to take images of the particles. All capturedimages can be displayed at once after testing. By analyzing the images of particles, information
such as the sizes and shapes of the particles can be obtained for analysis and display of histograms.
Particle images of up to 1,000 samples can be stored in the image storage device.
Other than PARTICLE SHEATH, four other types of alcohol can be used as dispersant.
The FPIA-3000S instrument is an improved version of the FPIA-3000 against the solvent resistant
characteristics, to expand the applicable dispersion medium over PARTICLE SHEATH and 4
kinds of lower alcohol.
Those particle that is difficult to disperse either in the PARTICLE SHEATH or the low alcohol or
those particle that is deformed by dissolving, shrinking or expanding can be analyzed by the FPIA-
3000S by dispersing in a suitable organic solvent that meets with the particle characteristics.
Dispersion medium of the PARTICLE SHEATH and variety of organic solvents can be used.You can view particle images and histogram analysis results of previously tested samples while
performing a test.
Cell size distributions and histogram analysis results can also be output to a printer or a host
computer.
Exterior view of this system and its peripherals
Figure 2-1: Testing unit and analysis unit of the FPIA-3000/3000S
Testing unit
Data processing unit
Monitor
Mouse
(Input device)
Keyboard
(Input device)Reagent
Pneumatic unit
7/18/2019 FPIA3000 User Manual English August 2006
2-4 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006
3.2 Screen display of the data processing unit
Figure 2-4: The display screen
(1) The menu bar
Menu items for operations that can be performed by the FPIA-3000/3000S are displayed here.
The items in the menu bar may be unavailable for selection, depending on the authorization
gained at logon, the screen currently displayed, the record data and other factors.
(2) Tabs
Tabs can be used to specify how analysis results should be displayed.
Item Tab content
Record List A tabular list of all analysis results stored in the DB is displayed.
Analysis Results Frequency distributions and other aspects of the analysis results are displayedfor the record selected in the record list.
Particle Image List All particle images are displayed for the record selected in the record list.
Frequency Table The frequency table for analysis results is displayed for the record selected inthe record list.
Meta Data Data such as system status and settings at the time of analysis are displayedfor the record selected in the record list.
Detail Results All analysis results for particle diameter and form parameters are displayed.
Multi Scattergrams The scattergram trend across multiple analysis results is displayed for therecords selected in the record list.
Graph(Overlay-View) The frequency distributions for multiple analysis results are displayedsuperimposed on one graph for the records selected in the record list.
Graph(Trend-View) The trend graph for multiple analysis results is displayed for the recordsselected in the record list.
Table 2-1: Tab display items
(1) The menu bar
(3) Status bar
(2) Tabs
7/18/2019 FPIA3000 User Manual English August 2006
2-6 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005
4. Emergency stop
If the instrument requires an emergency stop during testing or other operations, turn OFF the power
switch for the FPIA-3000/3000S testing unit.
5. Types of alarms
The FPIA-3000/3000S generates four types of alarms to indicate different state as described below.
(1) Error sound (peep)
This alarm sounds when some abnormalities occur in the testing unit. It will continue to sound
until the [Stop Alarm] button is pressed.
(2) Test start (pi pi pip)
This alarm sounds when a sample has been loaded into the instrument and the [Start] button
is pressed.
(3) Auto focusing (pip pip pip)
This tone sounds every time the auto-focusing mechanism moves one step during auto
focusing.
(4) Reverted from error (pip)
This sound is heard when the device reverts from an error.
WARNING• If the instrument fails and the organic solvent leaks inside the testing unit,
following error messages will be displayed. Immediately turn OFF the powerswitch of the FPIA-3000/3000S testing unit, and consult a Sysmex servicerepresentative for assistance.There is a potential to get fire or explosion when evaporated gas catches fire.
• Error messages when detecting leakage of the organic solvent:
Error message Description
Solvent is Leaked from fluid unit. A gas sensor detected a solventleakage in the hydraulic unit.
Solvent is Leaked from detector. A gas sensor detected a solvent leak inthe optical detector unit.
7/18/2019 FPIA3000 User Manual English August 2006
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005 2-7
6. Packaging
The instrument is thoroughly checked before it leaves the factory, and it is packaged with care to
withstand shock during transport. Once the instrument is delivered, check all packages to confirm
that none are damaged on the outside.
Unpacking, installation and confirmation of the initial operation are to be performed by servicepersonnel from your dealer or a Sysmex office. Each unit of the device is packaged separately.
7. Installation
7.1 Installing and moving the instrument
Installation of the FPIA-3000/3000S and its peripherals is to be performed by a Sysmex service
representative. If the device has to be moved after it has been installed, contact your Sysmex servicerepresentative.
Any problem caused as a result of moving it by the user will not be covered by the warranty even
if the problem occurs within the warranty period. Please note this.
7.2 Installation location
NOTE: See Appendix A, 1.1 Accessories for details of the accessories.
WARNING• Do not use fire when using flammable organic solvents. It may cause fire.• Please install the fire extinguisher suitable for the organic solvent near the
instrument.
WARNING • When an organic solvent is used as the sheath fluid, install a local ventilationdevice and make ventilation around the FPIA-3000/3000S. It may cause a fireor exposure to toxic substance.
• The organic solvent evaporated from the sample chamber is exhausted fromthe opening located center top of the FPIA-3000/3000S rear panel, shown in theFigure 2-5. When a local ventilation device is used, efficiently aspirate the gasexhausted from this opening with a duct.
Exhaust opening from the sample chamber
Figure 2-5: Exhaust Opening in the center top of the FPIA-3000S Rear Panel
2-8 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005
7.3 Grounding
The instrument power supply cord uses a three-prong plug. When the power supply socket is
provided with grounding, simply plug it to the socket. If the socket is not a three-prong grounded
socket, use the adapter plug provided and connect the ground cable.
Figure 2-6: Power Cord Plugs
8. Reagents
WARNING• The instrument must always be grounded when operated. Inadequate
grounding may cause electric shock.
CAUTION• The testing unit and pneumatic unit each have their own power cords.
Therefore, two power sockets are required.
CAUTION
• Do not spill the organic solvent nor the PARTICLE SHEATH. If spilt, turn OFF
the instrument power switch, disconnect the power cord, and take thenecessary action by referring to the MSDS (Material Safety Data Sheet).
• FPIA-3000S specifications are assured only when the PARTICLE SHEATH isused as the sheath fluid. When any other sheath fluid than PARTICLE SHEATHis used, the particle image may be different due to the refractive index of thesheath fluid used. Accordingly, the analysis results of the particle diameter andthe circularity may be different from those analyzed using the PARTICLESHEATH.
WARNING• When a flammable organic solvent is used as a sheath fluid, make a ventilation
around the instrument by installing a local ventilation device, if required. It maycause a fire or exposure to toxic substance.
• Do not use fire near the instrument when a flammable organic solvent is used
as a sheath fluid. It may cause fire.• In the case of fire, install a fire extinguisher suitable to the organic solvent to be
used.• When handling the organic solvent, follow the instructions given in the MSDS
(Material Safety Data Sheet).• When an organic solvent is used for the sheath fluid, after shutting down the
instrument, securely cap the solvent container and the waste container andstore them in a safe place. If the solvent container and the waste container areleft without capping, it may cause fire or explosion after gas is filled in the room.
7/18/2019 FPIA3000 User Manual English August 2006
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005 2-9
(1) sheath liquid (PARTICLE SHEATH)
The FPIA-3000 uses PARTICLE SHEATH as sheath liquid and rinse fluid. PARTICLE
SHEATH is aspirated into the instrument each time a test is conducted. For each test,
approximately 130 mL of PARTICLE SHEATH are used.
Figure 2-7: PARTICLE SHEATH container
(2) Sheath liquid (alcohol type)If alcohol is used as the dispersant in this instrument, the sheath liquid must be changed to the
same alcohol. Use alcohol as specified below.
(3) Sheath liquid (Organic solvent-only for FPIA-3000S)
When an organic solvent is used as a dispersion medium, use the same organic solvent as a
sheath fluid to form a stable flat sheath.
The FPIA-3000S uses a specific hydraulic part listed in the Table 2-4 “FPIA-3000S Hydraulic
Part Material” that is resistant to the organic solvent, to prevent those hydraulic part from being
affected when the organic solvent listed in the APPENDIX E, GAS SENSOR SETTING
VALUES AND LIST OF FPIA-3000S RECOGNIZED ORGANIC SOLVENTS is used as thesheath fluid.
However, the APPENDIX E, GAS SENSOR SETTING VALUES AND LIST OF FPIA-
3000S RECOGNIZED ORGANIC SOLVENTS shows the applicability and compatibility of
the hydraulic parts against the organic solvent, well focused particle images may not be
obtained depending on the viscosity and specific gravity of the organic solvent since a stable
sheath is not formed. If the ignition point, the boiling point, or the melting point is very low,
there is a potential of a fire or an explosion.
Alcohol Standard for compliance
Methanol JIS K 8891
Ethanol JIS K 8101
Isopropanol JIS K 8838
Ethylene glycol solution (25 wt%) JIS K 8105
Table 2-3: Alcohol compliance standards
NOTE: The FPIA-3000/3000S forms a flat sample flow to a flow cell to capture focused particleimages. The sheath liquid is the reagent used for forming the flat sheath flow.
PARTICLE SHEATH
7/18/2019 FPIA3000 User Manual English August 2006
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005 2-11
9. Names and functions of the components of the FPIA-3000/3000S
9.1 Front panel/right front panel
Figure 2-8: Front panel/right front panel of the testing unit
(1) STATUS lamp
You can judge whether the unit is ready to run tests. When the FPIA-3000 is ready to test, thislamp lights up in green.
(2) Electricity caution mark
(3) Cover open/close caution mark
(4) Mixing caution mark
WARNINGIf the organic solvent is used, discharge static electricity by touching anygrounded metal before starting to operate it. Otherwise, there is the risk of alcoholignition.
WARNINGThe testing unit cover opens and closes automatically during testing. Keep yourfingers well clear of the cover.
WARNING Keep clear of the mixing motor and mixing blades when pouring reagents.
(1) STATUS lamp
(2)
(3)
(4)
(5) Mixing motor
(6) Testing unit cover
(7) Pressure regulator unit cover
(8) Pressure regulator for rinsing
(9) Bellows pressure governor
(10) Power switch
7/18/2019 FPIA3000 User Manual English August 2006
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005 2-13
9.2 Rear side of the testing unit
Figure 2-9: Rear side of the testing unit
(1) Data communications connector (IOIOI)
Not normally used.(2) Electricity caution mark
(3) Video output connector ( )
Connected to a screen-output device such as a monitor, to check the image from the CCD
camera.
(4) Data communications connector ( )
Connected to the Data Processing Unit via the accessory cable.(5) Fuse holder
The fuse is connected here.
(6) Main power connector
Connected to the power socket via the accessory cable.
WARNINGIf touching the connector, discharge static electricity by touching any groundedmetal before starting to operate it. Otherwise, there is the risk of permanentdamage to the units.
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005
Chapter 3 PREPARATIONS FOR TESTING
1. Items to be checked before turning on the device....................................... 3-11.1 Reagents...............................................................................................3-1
1.2 Printer paper (when connected to a printer)..........................................3-11.3 Instrument inspection ............................................................................ 3-1
2. Turning on the device..................................................................................3-2
3. Starting the analysis program and background check ................................3-33.1 Starting the analysis program ...............................................................3-33.2 Sheath liquid replacement..................................................................... 3-53.3 Background Check................................................................................ 3-73.4 Auto Focusing ....................................................................................... 3-9
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005 3-1
1. Items to be checked before turning on the device
1.1 Reagents
1 Check that there is a sufficient amount of reagent for the scheduled tests.
Get a replacement reagent ready if there is not enough reagent.
2 Refer to Chapter 2, 8. Reagents, for the reagents that can be used as sheath liquid.
1.2 Printer paper (when connected to a printer)
When outputting the test results to a printer, check that the amount of printer paper is sufficient.
1.3 Instrument inspection
Check the tube and cable connections. Specifically, check that the connectors are firmly connected
and the tubes are not bent.
CAUTION
• Do not use a reagent that has passed its expiry date. The expiry date of each
reagent is written on the label of the container or on the box. Once the packageis opened, the contents must be consumed within 60 days.
• Leave the reagent container at room temperature before use. Use the reagentat 15–30°C.
• If there is a chance that the reagent might have frozen, leave it to reach roomtemperature, then shake the container well to homogenize the solution. Beforeusing the solution, make sure that there are no bubbles. Ignoring any of theabove precautions may result in inaccurate test results.
3-2 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005
2. Turning on the device
1 Testing Unit
Turn on the testing unit, referring to the diagram below.
Figure 3-1: Turning on the Testing Unit
2 MonitorSwitch on the power to the monitor as described in the manual for that monitor.
3 Data Processing Unit
Switch on the power to the unit as described in the manual for that PC.
When the data processing unit is turned on, Windows starts up.
NOTE: The power supply for the pneumatic unit is turned on automatically after the main unitpower is turned on and the FPIA-3000 application logon operation is complete.
Power switch
7/18/2019 FPIA3000 User Manual English August 2006
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006 3-3
3. Starting the analysis program and background check
The FPIA-3000/3000S uses rights management to limit its users. Refer to Chapter 13, USER
AUTHENTICATION for more on setting logon IDs and passwords etc.
3.1 Starting the analysis program
1 Double click on the FPIA-3000/3000S shortcut on the Windows desktop.
The logon dialog box appears.
2 Enter the logon ID and password, as in table 3-1, then press the [OK] button.
Figure 3-2: [Logon] dialog
NOTE: If the FPIA-3000/3000S does not start, use one of the methods below to start it.• Click through Windows taskbar – [Start] button – [Programs] – [Sysmex] – [FPIA-
3000].• Double click on [FPIA-3000.exe], which is directly below the install folder.
Set Items
Logon ID Enter the previously assigned logon ID.For details on input text format etc., refer to Chapter 13, USER
AUTHENTICATION .
Password Enter the previously assigned password.The input password will be displayed as a string of asterisks “*”.For details on input text format etc., refer to Chapter 13, USER
AUTHENTICATION .
[Start Testing Unit?]check box
When there is no need to start up the testing unit, for example when justreferencing data, put a check in “No (PC Only)”.
[OK] button This starts up the analysis program.
[Cancel] button Cancel FPIA-3000/3000S startup.
Table 3-1: Logon screen items
NOTE: The logon ID and password are case sensitive.
3-4 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006
NOTE: Use this for reference when logging in for the first time after the FPIA-3000/3000Sapplication is installed.
• General user password registrationWhen installing, use the following for reference and set user name and password. Beaware that only the login with administrator group authority allows you to set apassword.
1) Select [Settings] – [User Authentication] – [User Registration] and execute.2) Press the [Add] button on the user information tab and set the logon ID and
password here. For the more detailed procedure, refer to Chapter 13, USER AUTHENTICATION .
• System administrator password registrationThis system is set at the factory with the following registered as the logon ID for thedefault administrator group.
Logon ID: admin
Password: admin
The following logon IDs are also registered as defaults.
Logon ID: guestPassword: guest
Logon ID: managerPassword: manager
Use [Settings] – [User Authentication] – [User Registration] to make changes asappropriate.(Log on with Logon ID: Admin and Password: Admin, create the logon user with theadministrator group, logon again with the logon user you just created, then change the
password etc. above.)
When you start up the FPIA-3000/3000S, the Windows logon ID appears in the [LogonID] field of the logon dialog box.Registering the same ID with the Windows logon ID saves entering the ID when youstart up the FPIA-3000/3000S.
CAUTION[Setting] button• Setting button is only available when the HIGH/LOW MAGNIFICATION LENS
UNIT or the DARK FIELD UNIT is mounted.• Refer to Appendix H, HIGH/LOW MAGNIFICATION LENS UNIT MANUAL
(OPTION) and Appendix J, FPIA-3000 DARK FIELD UNIT MANUAL for detailsof each unit.
3. Test information setting ............................................................................... 4-43.1 Sample information setting.................................................................... 4-43.2 SOP setting ........................................................................................... 4-53.3 Measurement condition settings ........................................................... 4-63.4 Analysis conditions................................................................................ 4-83.5 Setting instrument conditions ................................................................4-9
4. Test procedure .......................................................................................... 4-114.1 Preparing a sample ............................................................................. 4-114.2 Testing a sample.................................................................................4-12
5. Display of analysis results ......................................................................... 4-14
6. Shutting down (turning off the power) ....................................................... 4-15
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005 4-3
3 Select [Test]–[Test].
The [Testing/Analysis Parameters] dialog appears.
4 Check that the status for each unit is green.
The statuses will not all turn green unless the background check, auto focus adjustment, etc. are
executed.
Figure 4-4: [Testing/Analysis Parameters] dialog
5 If the status is red, take appropriate action, using the following as reference.
Status in dialog Reason displayed Page reference
Next to the [CheckBackground] button, “NotTested” is displayed in red.
This is displayed if nobackground check has beenexecuted since the testingunit power was switched ON.
Press the [Check Background] buttonin the dialog and execute thebackground check.
Next to the [Auto Focus]button, “Not Tested” isdisplayed in red.
This is displayed if the autofocus has not been adjustedsince the testing unit powerwas switched ON.
Press the [Auto Focus] button in thedialog and start the auto focusadjustment sequence. For details onhow to adjust the sample for auto focusadjustment and how to load it into thedevice, refer to page 3-9 in Chapter 3.
Next to the [Pneumatic Unit]button, “Sleeping” isdisplayed in red.
This is displayed if thepneumatic unit is OFF.
Refer to page 14-1 in Chapter 14 andmake the pneumatic unit run.
Next to the [Replace SheathLiquid] button, the name ofthe sheath liquid is displayedin red and under the [Autofocus] button, “Differentsheath liquid loaded indevice” is displayed in yellow.
This is displayed if the sheathliquid selected in SOPregistration with the [Testing/Analysis Parameters]dialog and the sheath liquidcurrently loaded in the testingunit are different.
Replace the sheath liquid with the oneset for the SOP measurement.Alternatively, change the measurementconditions for the SOP settings in the[Testing/Analysis Parameters] dialogand measure with the SOP setting listbox not set.
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005 4-7
NOTE: If you select [Apply] in [Testing Parameters] - [Auto Merge] on the SOP Settingsdialog, and use repeat measurement on Stop by Time method, measured records will bemerged automatically and merge recode will be generated when all repeatmeasurement finished.
CAUTIONWhen running repeated tests, check the remaining volumes of reagents beforestarting each test. If an error such as insufficient reagent occurs during a test, thatand subsequent tests will be invalid.
NOTE: How to cancel auto repeat measurement• Select [Test] – [Cancel Repeat Tests].
Then the following message appears.
Figure 4-8: [Repeat Testing] dialog
• Click on the [OK] button to abort auto repeat measurement.Auto repeat measurement will stop before the measurement after next.
Also, to stop measurement immediately, select [Test] – [Cancel Test].Then the following message appears.
Figure 4-9: [Testing in progress] dialog
When you press the [OK] button, measurement is stopped immediately. (Depending onthe timing, measurement may be stopped at the start of the next measurement.)
7/18/2019 FPIA3000 User Manual English August 2006
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005 4-9
6 Click on and under [Dilution Ratio] to enter the ratio.
Values can also be entered directly from the keyboard.
When the dilution ratio is set, the particle density is converted to the pre-dilution value and
displayed.
3.5 Setting instrument conditions
Set instrument conditions, as described below.
Figure 4-11: Testing Parameters
CAUTION
If you click [Set Detail] button and select [Analyze] in [Testing Parameters] -
[Merge(LPF+HPF)] on the Testing Detail Parameters dialog, size parameter canbe selected from only CE diameter (number base, area base, and volume base).After this measurement, recodes (the measurement mode of which is aMerge(LPF+HPF)) which are from LPF measurement result recode, HPFmeasurement result, and Merge(LPF+HPF) mode will be generated. refer toChapter 16, FUNCTIONAL DESCRIPTION about full range.
4-10 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006
1 Click on the [Set Detail] button.
The [Testing Detail Parameters] dialog appears.
Figure 4-12: [Testing Detail Parameters] dialog
2 Set the setting values as required.
Scroll the list up and down to display the item you want to change. Move the mouse to the value
field for the item you want to change, then left click. It becomes possible either to select from a list
box for that value area or to change the value directly. Here, change the item or change the value.
After the change, when you press [Close], the measurement parameter is changed.NOTE: Refer to Appendix C for the meanings of setting values.
CAUTIONIf you change a measurement condition with the “Edit Testing/AnalysisParameters” check box in the [Testing/Analysis Parameters] dialog checked,the inside of the SOP setting selection list box in the SOP settings turns white andSOP is deselected. If you measure in this state, measurement uses the currentsettings not the existing SOP conditions. If you want to re-analyze themeasurement results with the existing SOP conditions, refer to Chapter 5,2.5.1. Restricted analysis within rectangular range.
4-12 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005
4.2 Testing a sample
1 After preparing the sample to test, press the [Start] button on the [Testing/Analysis
Parameters] screen.
Figure 4-13: [Testing/Analysis Parameters] dialog
After processing of the previous sequence is complete, the following dialog appears, and theTesting Unit cover opens automatically.
Figure 4-14: [Measurement Sequence] dialog
CAUTIONWhen measuring highly adhesive particles or samples with high particleconcentrations, check to make sure that there is no carryover. Ordinary rinsingmay not be sufficient. When there is carryover, perform the automatic rinsingsequence. (Refer to Chapter 15, Auto rinse.)
CAUTIONFor safety’s sake, do not open or close the cover by hand.
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005 4-13
2 Dispense 5 ml of the prepared sample into the testing unit sample chamber, and press the [Start]
button. The cover closes automatically, and measurement starts.
Figure 4-15: Setting the sample to be tested
3 After the test is finished, the Record List is updated to display the latest analysis results.
WARNING• Keep your hands clear of the moving cover. There is a potential to get injured.• Be careful not to touch the moving agitator. There is a potential to get injured.
7/18/2019 FPIA3000 User Manual English August 2006
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005 4-15
6. Shutting down (turning off the power)
1 Select [File] – [Exit].
The following dialog will be displayed.
Figure 4-18: [Shutdown] dialog
2 Click on the [ Yes] button.
The dialog below appears, and the shutdown process runs.
Figure 4-19: [Shutdown Sequence] dialog
3 The testing unit cover opens, so add 10 mL of RO water to the sample chamber and then click
the [OK] button.
If particle sheath is being used as the sheath liquid, after the shutdown operations end, the testingunit cover opens, so dispense 10 ml of RO water into the sample chamber, then switch OFF the
testing unit power. If you are using a sheath liquid other than particle sheath, the testing unit
cover does not open. Also, it is not necessary to dispense the RO water.
NOTE: The effective analyzed number which is entered to the FPIA-3000 will be shown inmeasurement count area per 1 second. In Stop by Time mode, count number will bereset to 0 by repeating. However, Stop by count always indicates whole count number.
NOTE: Shut down the testing unit and the data processing section. After shutdown, the dataprocessing section automatically ends its program.
7/18/2019 FPIA3000 User Manual English August 2006
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006
Chapter 5 RECORD DISPLAY AND PROCESSING
1. Record Display and Processing .................................................................. 5-11.1 Processing the record list...................................................................... 5-1
1.2 Screen composition............................................................................... 5-11.3 Explanation of functions ........................................................................5-21.4 Sort functions ........................................................................................ 5-71.5 The filter function................................................................................... 5-91.6 Search functions .................................................................................5-111.7 Display reset .......................................................................................5-131.8 Sample information revision................................................................5-141.9 Reanalyze ........................................................................................... 5-151.10 Deletion .............................................................................................5-161.11 Merging records ................................................................................5-161.12 Copying to the clipboard ................................................................... 5-17
1.13 Changing the record display .............................................................5-172. Processing analysis results .......................................................................5-18
2.1 Introduction ......................................................................................... 5-182.2 Screen composition............................................................................. 5-182.3 Moving records.................................................................................... 5-202.4 Thumbnail image display .................................................................... 5-212.5 Reanalyze ........................................................................................... 5-222.6 Selection particle save ........................................................................5-342.7 Changing the display of the Analysis results screen........................... 5-34
3. Particle image list ...................................................................................... 5-35
3.1 Introduction ......................................................................................... 5-353.2 Screen composition............................................................................. 5-353.3 Copying to the clipboard .....................................................................5-363.4 Detailed information display ................................................................ 5-37
4. Frequency tables....................................................................................... 5-38
4.1 Introduction ......................................................................................... 5-384.2 Screen composition............................................................................. 5-384.3 Copying to the clipboard .....................................................................5-38
5. Multi Scattergrams ....................................................................................5-395.1 Introduction ......................................................................................... 5-39
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006
6.3 Copying to the clipboard ......................................................................5-416.4 Setting up display ................................................................................5-41
7. Meta Data ..................................................................................................5-427.1 Introduction..........................................................................................5-427.2 Screen composition .............................................................................5-42
7.3 Copying to the clipboard ......................................................................5-42
8. Detail Results.............................................................................................5-438.1 Introduction..........................................................................................5-438.2 Screen composition .............................................................................5-438.3 Copying to the clipboard ......................................................................5-43
9. Graph (Trend-View) ...................................................................................5-449.1 Introduction..........................................................................................5-449.2 Screen composition .............................................................................5-449.3 Copying to the clipboard ......................................................................5-459.4 Setting up display ................................................................................5-45
10. The File menu..........................................................................................5-4510.1 File menu items .................................................................................5-4510.2 New....................................................................................................5-4610.3 Open..................................................................................................5-4710.4 Import.................................................................................................5-4810.5 Save ..................................................................................................5-5010.6 Printing...............................................................................................5-5210.7 PDF Print ...........................................................................................5-5310.8 CSV export ........................................................................................5-5510.9 Transmission to the host....................................................................5-56
5-2 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006
1.3 Explanation of functions
(1) Functions of buttons on the [Record List] screen
(2) Menu > Record processing
(3) Navigation to other screens (when one record is selected)
If you left click a record with the mouse on the record list screen, that record is displayed inverted
in blue and that record is selected.
Double click on the record to display the [Analysis Results] screen. Click on a tab on the screen
to display the next screen, which will display the content of the selected record on the
corresponding screen.
• Analysis Results screen
• Particle Image List screen• Frequency Table screen
• Meta Data screen
• Detailed Results screen
• Multi Scattergrams screen
• Graph(Overlay-View) screen
• Graph (Trend-View) screen
NOTE: When the maximize button in the FPIA-3000 window, a List screen can be displayed onthe CRT in the maximal size. When the maximize button is repressed, the display showsthe original screen.
Item Functions
Reset Display Cancels sorting and filtering settings, searches etc. and returns the screen tothe default display.
Search Search for records.
Sort Set the sorting method.
Filter Set the filtering method.
Table 5-1: [Record List] screen items
Item Functions
Select all Selects all the records.
Corresct SampleInformation
Revise sample information within the record information.
Reanalyze Select records and reanalyze them as a batch.
Delete Delete the selected record.
Merge Merge the selected record.
Table 5-2: [Record List] screen items
7/18/2019 FPIA3000 User Manual English August 2006
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006 5-3
(4) Navigation to other screens (when multiple records are selected)
Multiple records can be selected using the mouse and keyboard. Click on multiple non-contiguous
records while holding down the Ctrl key to select them. The mouse can also be dragged across
multiple contiguous records to select them all. The selected records are highlighted in reversed
color. To cancel the selections, release the keyboard Ctrl key and select another record.
After selecting multiple records, clicking a tab on the screen, and displaying the [AnalysisResults] screen, [Particle Image list], [Frequency Table], meter data, or [Details Results]
screen, of the records selected on the record screen, the one displayed at the top is the one whose
contents are displayed. When a [Graph (Trend-View)] screen, [Multi Scattergrams] screen,
or [Graph (Overlay-View)] is displayed, the graph is drawn or the scattergram or the like
displayed using the contents of the selected records.
(5) Fixed display of column items
The column items that are always displayed on the [Record List] screen can be fixed in position.
For the procedure to set column positions, refer to Chapter 11, 1.1 View settings – Record list .
(6) Selection of column display itemsThe column items to be displayed and hidden on the [Record List] screen can be set, as well as
their alignment. For the procedure to set column positions, refer to Chapter 11, 1.1 View settings –
Record list .
(7) Change display item width
The widths of columns that are displayed on the [Record list] screen can be altered. Left click on
the column to change, and drag the pointer left and right to change to the desired width.
(8) Record data display contents
The following information is displayed in the list. The default is to display those items with o in the
default display column.
Displaycontents
Detailed item Defaultdisplay
Explanation
RecordInformation
Record Number O This is a unique number for each measurement resultrecord.
Tested O This is the date and time of the measurement.
Reanalyzed O This is the date and time of the reanalysis.
Re Analysis Checked if the data was reanalyzed.
Transmitted Checked if the record has been sent to the host.
Printed Checked if the record has been printed out.
Error Checked if an error occurred that affected themeasurement data.
External Save Checked if the record was stored externally.
5-4 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006
UserInformation
Measure name O The logged-in user name at the time of themeasurement is displayed.
Re Analyzed By The logged-in user name at the time of the reanalysis
is displayed.SampleInformation
Sample name O The name of the sample set at the time ofmeasurement is displayed.
Sample number O The number of the sample set at the time ofmeasurement is displayed.
Sample Type The type of the sample set at the time ofmeasurement is displayed.
Comment The comment concerning the sample information setat the time of measurement is displayed.
TestingParameters
OLens O lens information used in the measurement isdisplayed.
*For details of the O lens, refer to Appendix H, HIGH/ LOW MAGNIFICATION LENS UNIT MANUAL(OPTION).
Light fieldsystem
O Light field system information used in themeasurement is displayed.*For details of the Light filed system, refer to Appendix J, FPIA-3000 DARK FIELD UNIT MANUAL.
SOP O The SOP group name set at the time of themeasurement is displayed.
Magnification O The measurement mode (LPF/HPF/LPF→HPF) at thetime of measurement is displayed.
Count method O The count technique (total count/quantity count) at thetime of measurement is displayed.
Selection dataSave
Checked if this record is data saved (see 2.6Selection particle save)
Merge data Checked if this record is merged data(see 1.11Merging records)
AnalyzedResults
Density O For the contents, refer to Chapter 16, 5. List of displayitems.
Selected (%) O For the contents, refer to Chapter 16, 5. List of displayitems.
Small (%) For the contents, refer to Chapter 16, 5. List of display
items.Middle (%) For the contents, refer to Chapter 16, 5. List of display
items.
Large (%) For the contents, refer to Chapter 16, 5. List of displayitems.
5-10 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006
Item Detailed item Functions
Select Filters Check to use filtering.
Date Check to use the test date as a filtering condition.
Today Check to specify dates up to today corresponding withthe Windows system date.
Date Check to specify the start date and end date.
Detector and SOP Detector OLens can be selected from OLensUnit 5X, 10X and20X. Light field system (Bright field/Dark field) isselectable only when the OLens Unit 10X is selected.
SOP Only the registered SOP can be selectable.
Reagent name Reagent name which is set in Testing/AnalysisParameters Dialog can be selected.
Power Field Power Field(HPF,LPF,LPF->HPF) which is set inTesting/Analysis Parameters Dialog can be selected.
Count method Count method(Stop by count,Stop by Time) which is set
in Testing/Analysis Parameters Dialog can be selected.
Reanalysis Check to specify by the data attributes of test data.
Tested Data Check to specify test data.
Reanalyzed Data Check to specify reanalyzed test data.
Testing Status Check to specify by test status.
Normal Check to specify tests with normal samples.
Error Check to specify only samples with test errors.
Report Status Check to specify by test result.
Reported Check to specify only data for which test results havebeen printed out.
Not Reported Check to specify data for which test results have notbeen printed out.
TransmissionStatus
Check to specify by transmission status.
Transmitted Check to specify only data for which test data havebeen transmitted.
No Transmitted Check to specify test data that has not beentransmitted.
Table 5-5: Filtering function items
7/18/2019 FPIA3000 User Manual English August 2006
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006 5-11
2 Set the conditions.
3 Click on the [OK] button.
The records are extracted and displayed as specified.
1.6 Search functions
Search for any target record from among the items displayed on the [Record List] screen. Search
targets are those for which match from the first character (wild card characters cannot be used). The
search makes a distinction between single-byte and double-byte characters as the input search key.
1 Enter the search string in the [Search] field of the [Record List] screen.
Figure 5-5: Search settings
NOTE: All conditions are [AND] conditions.ExampleTest date specification: Today (Windows system date is 2004/01/01)Detector and SOP - SOP: HPF standardRunning filtering with the above settings extracts and displays only those records for
which the measurement date is 2004/01/01 and the SOP group is HPF Standard.
7/18/2019 FPIA3000 User Manual English August 2006
5-12 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006
2 Press the [Search] button.
If there are any matching records, the cursor moves to a highlighted item.
Figure 5-6: Search results
NOTE: • If there are no matching records, the following dialog appears. Please search again.
Figure 5-7: If the search found no matching records
• The cursor moves down from its present position in order, and when the cursor comesto the bottom of the records, the cursor starts searching from the top of the records.
7/18/2019 FPIA3000 User Manual English August 2006
The [Correct Sample Information] dialog will be displayed.
Figure 5-9: [Correct Sample Information] dialog
2 Set the items.
3 Click on the [Correct] button.
The [Confirm Sample Information] dialog will be displayed.
Figure 5-10: [Confirm Sample Information] dialog
4 Click on the [ Yes] button.
The record sample information is corrected.
NOTE: The [Correct Sample Information] dialog can also be called by right clicking on therecord on the [Record List] screen, and selecting [Correct Sample Information] fromthe context menu.
Item Functions
Sample Name Select the registered sample name.
Sample Number Enter the sample No.
Sample Type Enter the sample group type.
Comments Enter a comment.Up to 50 characters can be entered.
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006 5-15
1.9 Reanalyze
Selected records can be reanalyzed in a batch.
1 Select [Menu] – [Record] – [Reanalyze].
The [Analysis] dialog will be displayed.
Figure 5-11: [Analysis] Dialog
2 Select the above item. Press the[Analyze] button.A dialog is displayed to confirm that you want to execute reanalysis. To execute, press
[Analyze]; to cancel, press [Cancel].
When you press [Analyze], the specified data is reanalyzed in one batch.
NOTE: The [Analysis] dialog can also be called by right clicking on the record on the [RecordList] screen, and selecting [Analyze] from the context menu.
Item Functions
Combo box The data can be changed to a registered SOP.
Save New(Radio button)
Select if the reanalyzed data is to be saved as new test data when thereanalysis is conducted.
Save (Radio button) Select if the reanalyzed data is to be saved by overwriting when the reanalysisis conducted.
Table 5-7: [Analysis] dialog screen items
7/18/2019 FPIA3000 User Manual English August 2006
5-16 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006
1.10 Deletion
Selected records can be deleted. Select beforehand any records you want to delete.
1 Select [Menu] – [Record] – [Delete].
The [Deletion of Selected Data] dialog will appear for the selected data.
Figure 5-12: [Deletion of Selected Data] dialog
2 Click on the [OK] button.
The selected data is deleted. Press the [Cancel] button to stop processing and return.
1.11 Merging records
You can merge multiple records.
1 On the [Record List] screen, select the records for analysis.
2 Select the [Record] -> [Merge] or right-click on the recode list and select merge.
The following dialog will be displayed.
Figure 5-13: [Setting Sample Information] dialog
NOTE: The [Deletion of Selected Data] dialog can also be called by right clicking on a recordon the [Record List] screen, and selecting [Delete] from the context menu.
Item FunctionsSample Name Select a previously registered sample name or enter a new sample name.
Up to 50 characters can be entered.
Sample Number Enter the sample No.Up to 50 characters can be entered.When you input the numeric value, it is counted up each time there is ameasurement.
Table 5-8: Merge settings
7/18/2019 FPIA3000 User Manual English August 2006
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006 5-17
3 Set the items.
4 Click on the [OK] button.
The records are merged with the set content.
Press the [Cancel] button to stop processing and return.
1.12 Copying to the clipboard
Selected records can be copied to the clipboard. Records copied to the clipboard can be pasted into
other application software. String names can also be copied to the clipboard, as record headers.
1 Right click on a record in the [Record List] screen, then select [Copy to clipboard] from the
context menu.
The record is copied to the clipboard.
1.13 Changing the record display
The items displayed in the record list can be changed. For details, refer to Chapter 11, 1.1 View
settings – Record list .
Sample Type Enter the sample group type information.
Comments Enter a comment.Up to 50 characters can be entered.
CAUTIONData from different Power Field (LPF/HPF) cannot be merged.LPF→HPF (HPF) and HPF are handled as the same mode and LPF→HPF (LPF)and LPF are handled as the same mode.Data with different Count method (total mode/quantity count mode) cannot bemerged.Data reanalyzed from a record cannot be merged with the original record.
NOTE: You can merge select [Menu]-[Recode]-[Merge] after you get analysis result byselecting multiple recodes in record list.
NOTE: Clipboard: An area in main memory (within the PC) used for temporary storage of data.
5-18 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006
2. Processing analysis results
2.1 Introduction
This section explains the [Analysis Results] screen.
The [Analysis Results] screen displays analysis results for each record that was selected on the[Record list] screen. If multiple records are selected, analysis results are displayed for the
uppermost of the records selected on the [Record List] screen.
2.2 Screen composition
Figure 5-14: [Analysis Results] screen
Sample Information
Restricted analysis range
change combo box
Record
selection
Scattergram Test value display area
Particle diameter
Histogram
[Particle diameter
selection] button
[Image] button [Save] button
[Reanalyze] button
[Particle formselection] button
Particle form histogram
7/18/2019 FPIA3000 User Manual English August 2006
Test value display area The test values for the selected record are displayed.When you press thestandard tab, analysis data and particle form date for the normal particlediameter is displayed. Also, if you press the user defined tab, the userdefined data registered with [Settings] – [SOP] is displayed. For details onhow to set user definitions, refer to Appendix C, 2. Table of SOP settings.When you press the Cumulative Frequency tab, the Cumulative Frequency(X axis) is displayed by 10%. When you right click on the test value display
area, "Copy to clip board" or "View Settings (Result list)" can be selectedand done. If the "View Settings (Result list)" is displayed, you can changethe location of the result list parameter display. For details, refer to Chapter11, 1.3 View settings – Result (List).
Table 5-9: Analysis results screen items
NOTE: User Information items are a function that registers formulas using measured analysisparameters selected by the user and adds the results of calculating the formula to themeasurement record. For further details, refer to Appendix C, 2. Table of SOP settings.
CAUTION • In the Merge(LPF+HPF) mode, the [Image] button on the Analysis ResultsScreen is disabled. (Image display function is not provided.) Furthermore, theeffective analyzed number and the selected number are not displayed.
• When the analysis results of the data in the Merge(LPF+HPF) mode aredisplayed, the particle image list tab, the Merge(LPF+HPF) tab and imagesduring multi scattergram cannot be displayed. Also, the particle images cannotbe printed out.
• For details of the Merge(LPF+HPF) mode, refer to Chapter 16, FUNCTIONALDESCRIPTION .
5-22 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006
2.5 Reanalyze
You can reanalyze the measurement results with the method below by pressing the [Reanalyze]
button on the [Analysis Results] screen to open the reanalysis screen.
At the right of Figure 5-17 Reanalysis screen, there are the standard tab and enhancing 1-5 tabs.
If you press one of the buttons, following analysis can be done separately.
• You can change the limit axes (X axis, Y axis), which are analysis parameters, and reanalyze.
• You can restrict the limit axis (X axis, Y axis) analysis range to any area you desire and reanalyze.
• You can change the cumulative frequencies for the lower value and upper value of the analysis
results to any desire values and reanalyze.
With the standard button, you can use the mouse to move the restriction area axes (X axis and Y
axis) for the restricted axis particle distribution histogram to specify a rectangular area within the
scattergram and execute an analysis restricted to within that rectangular range. For the concrete
procedure, refer to Chapter 5, 2.5.1. Restricted analysis within rectangular range. Also, the results
of this analysis are saved in the measurement record.
With enhancing analyses 1-5, in addition to analysis restricted to a range, you can analyze restrictedto a polygon range by specify the polygon range within the scattergram and executing the restricted
analysis. For the concrete procedure, refer to Chapter 5, 2.5.2. Polygon range limit analysis.
• By selecting a registered SOP, you can reanalyze with the analysis conditions set for that SOP.
• You can register any analysis conditions you want as a new SOP. This means that you can
measure next time under the same conditions if you use prior SOP.
2.5.1. Restricted analysis within rectangular range
The [Reanalyze] button on the [Analysis Results] screen can be used to reanalyze test results.
Press the [Cancel] button to interrupt the reanalysis.
You can analysis with various items by pressing the standard button or one of the expansionbuttons, 1-5, in the figure.
NOTE: Polygon range limit analysis is selected by default only Exhancing 5, Reconfigure inDetailed Settings if necessary. For details, see Appendix C, TABLE OF SYSTEMSETTINGS.
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006 5-23
Figure 5-17: Reanalysis screen
Item Functions
[Image] button Image thumbnails of the selected records can be displayed.
[Cancel] button Cancel the analysis.
[Make New SOP] button A new SOP can be made.
[Clear Area] button The preset SOP information area can be cleared.
[Detailed Settings] button The detailed information on the selected SOP can be displayed.
Table 5-11: Functions of reanalysis buttons
CAUTIONWhen you press the tab button such as “Record List” without pressing the“Cancel” button while the analysis is executed, the analyzed data storage dialogwill be displayed. “The reanalyzed data is not saved. Update?” appears. Note thatthe reanalyzed data is not saved and the display goes to each tab screen if youpress the [NO] button here.If you press the [YES] button here, the reanalyzed data will be saved and thedisplay goes to each tab screen.
X-axis particle distribution histogram
Y-axis particle distribution histogram Scattergram display Standard and Analysis 1 – 5 buttons
7/18/2019 FPIA3000 User Manual English August 2006
5-24 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006
1) Limit axis (X, Y axis) change and lower (%) value and upper (%) value change
Records can be reanalyzed by pressing the [Reanalyze] button on the [Analysis Results]
screen. Changing the value of the list box of limit axes (X and Y axes) reanalyzes with the
applicable analysis parameters and displays the results.
The limit axis information can be set separately between standard analysis and enhancing analysis 1-5.
By dragging the left side or right side vertical axis of the X-axis particle distribution graph (leftclicking the mouse and moving it) you can move the area limit axes and can limit the range of the
area. (By default, the area outside the limits is grayed out.) The particles inside the area are subject
to analysis.
By dragging the top side or bottom side horizontal axis of the Y-axis particle distribution graph (left
clicking the mouse and moving it), you can move the area limit axes and can limit the range of the
area. (By default, the area outside the limits is grayed out.) The particles inside the area are subject
to analysis.
At this time, the values inside the limited analysis range change combo box change to show the
position of the area limit axes.
You can also change the area limit axis by changing the values in this combo box. In this case, the
limit axis is moved automatically.
Figure 5-18: Limit axis parameter change list box
Limit axis parameter change list box (for X axis)
Limit axis parameter change list box (for Y axis) Restricted analysis range change
combo box
7/18/2019 FPIA3000 User Manual English August 2006
5-30 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006
5 Set the items.
6 After changing the SOP parameters, click on the [Make New SOP] button.
The following dialog will be displayed.
Figure 5-28: [new SOP registration] dialog
7 Enter the SOP name for new registration, then click on the [OK] button.
The following dialog will be displayed.
Figure 5-29: [Registration of New SOP] dialog
8 Click on the [OK] button.
Reanalysis will be carried out with the new SOP, and the newly-registered SOP conditions willappear in the SOP list box.
Figure 5-30: Display of Analysis Parameters after SOP registration
When you press the [Save] button to open the data storage dialog, select Update or New and press
the [Save] button, the reanalyzed results are saved.
NOTE: This dialog can be used to set the particle analysis conditions, statistical analysisconditions and limit analysis conditions that are required for the analysis process. TheSOP for test conditions etc. can be changed to other SOP conditions using [Settings] –[SOP settings]. However, these parameters will not have any impact on the analysis.
NOTE: You can save the changed reanalysis conditions or create new records without settingan SOP. In that case, the SOP list box is blank.
7/18/2019 FPIA3000 User Manual English August 2006
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006 5-33
Figure 5-35: Selecting a polygon range vertex
Bring the mouse to the vertex that has become a square and while holding down the left mouse
button at that location, move the mouse to move the vertex as in Figure 5-36.
Figure 5-36: Moving a polygon range vertex
Each time the polygon range is changed, the statistics are analyzed and the results are displayed in
the analysis results display area.
CAUTIONWhile the analysis is executed, press the Multi Scattergram tab, the Graph(Overlay-View) tab, Graph (Trend-View) tab or the Record list tab withoutpressing the [Cancel] button. Then, the analyzed data storage dialog will bedisplayed. “The reanalyzed data is not saved. Update?” appears. Note that thereanalyzed data is not saved and the display goes to each tab screen if you pressthe [NO] button here.If you press the [YES] button here, the reanalyzed data wil be saved and thedisplay goes to each tab screen.
5-34 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006
2.6 Selection particle save
After analysis is performed in the particle image list window, the results about the particles of the
marked particle image can be stored.
Press the [Save] button on the analysis result screen.
Figure 5-37: [Data storage] dialog
When you select the [Selection particle Save] from this dialog, the selection particle list box isenabled and you can select among All selection particles, Marked particles and No marked particles
to store.
When you select an item and press the [Save] button, the particles to be stored will be reanalyzed
and its results will be stored in the new record.
2.7 Changing the display of the Analysis results screen
The display colors on the Analysis results screen can be changed. For details, refer to Chapter 11,
1.2 View settings – Result (Graph).
Item Function
All selection particles All particles in the area can be stored.
Marked particles The selected particles with mark selected in the particle image list can bestored.
No marked particles The selected particles other than the ones with mark selected in the particleimage list can be stored.
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006 5-35
3. Particle image list
3.1 Introduction
This section explains the [Particle Image List] screen.
The [Particle Image List] screen can display the particle images for the test data selected fromthe [Records List] screen.
3.2 Screen composition
Figure 5-38: [Particle Image List] screen
Item Functions
Sort This function sorts and displays image data.
Particle Size The displayed size of the images can be altered. Images can be listed at fullsize, 2×, 4×, 8× and 10×.
Display Param: Do not display: Particle images are displayed without parameters. Limitingparameters: The limit axis parameters are displayed under each particle image.
Table 5-13: [Particle Image List] screen items
7/18/2019 FPIA3000 User Manual English August 2006
5-36 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006
Figure 5-39: Parameter display screen
3.3 Copying to the clipboard
Right click the image list display to copy images to the clipboard.
Figure 5-40: Popup menu in the [Particle Image List] screen
Mark limitation When you select an item among No limitation (images are displayedregardless of the marks), Mark display (only the marked images are displayed)and Display outside mark (images without marks are displayed), you candisplay the selected type screen.When a particle image is clicked on, the border of the image turns red
(marked). Click again reverts to the previous color. The marked particle isdisplayed marked in the Multi Scattergram screen display of the [AnalysisResults] screen.The information about Marking is saved in the Record.
Boundaryline The particle image data can be displayed as outlines
NOTE: Clipboard: An area in main memory (within the PC) used for temporary storage of data.
Item Functions
Copy To Clipboard(1 Particle)
Copy particle images for single particles to the clipboard.
Copy To Clipboard (1 page) Copy all displayed particle images to the clipboard.
Detail Information Display detailed information on the corresponding particles on thescreen.
Table 5-14: [Particle Image List] screen pop-up menu items
Item Functions
Table 5-13: [Particle Image List] screen items
Displays parameters of each axis
7/18/2019 FPIA3000 User Manual English August 2006
5-38 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006
4. Frequency tables
4.1 Introduction
This section explains the [Frequency Table] screen.
The [Frequency Table] screen can be used to display frequency tables for the records selectedon the [Record List] screen. The frequency table list on the X-axis limited range, the frequency
table list on the Y-axis limited range, and the scatter frequency table with limits are displayed.
4.2 Screen composition
Figure 5-42: [Frequency Table] screen
4.3 Copying to the clipboard
The displayed frequency table data can be copied to the clipboard.
1 Right click on a frequency table, then select [Copy To The Clipboard] from the context
menu. The data is copied to the clipboard.
NOTE: Clipboard: An area in main memory (within the PC) used for temporary storage of data.
7/18/2019 FPIA3000 User Manual English August 2006
5-40 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006
5.4 View settings
Right click on the Multi Scattergram screen. Select “View Settings(Result Graph)” from thedisplayed menu. You can do view settings in the Multi Scattergram screen. For details, refer to
Chapter 11, DISPLAY SETUP .
5.5 Image
Click an optional scatter in the Multi Scattergram screen and right click to display the menu. Select
“Image” from the displayed menu.
The corresponding scatter records are displayed as thumbnails.
Furthermore, if you double click an optional scatter, the image thumbnails will be displayed as
above.
5.6 Image (Reference)
Click an optional scatter in the Multi Scattergram screen and right click to display the menu. Select
“Image (Reference)” from the displayed menu.
The corresponding scatter records are displayed as thumbnails.
6. Graph (Overlay-View)
6.1 Introduction
This section explains the [Graph(Overlay-View)] screen.
The [Graph (Overlay-View)] screen can display the particle diameter parameters and particle
form parameters for up to ten records selected on the [Record List] screen.
This displays an overlay graph of the frequency distribution with the X-axis limited, the cumulative
distribution, and the frequency distribution with the Y-axis limited. This is effective for looking at
particle differences.
NOTE: Clip board is an area on the main memory (inside the PC) where data is temporarilystored.
CAUTIONFor the image thumbnails of the Multi Scattergram, one image window and oneimage (reference) window, or total 2 windows can be displayed. You cannotdisplay one scatter as the image and image (reference).
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006 5-43
8. Detail Results
8.1 Introduction
This section explains the [Detail Results] screen.
On the [Detail Results] screen, the analysis results, the sample information, the particle diameterparameters analyzed statistically, and the particle form parameters analyzed statically for the
records selected on the [Record List] screen can be displayed.
The parameters selected as a limit axes display statistical analysis items restricted, and the others
display the items not restricted.
8.2 Screen composition
Figure 5-46: [Detail Results] screen
8.3 Copying to the clipboard
The data items on the [Detail Results] screen can be copied to the clipboard.
1 Right click on the [Detail Results] screen, then select [Copy To The Clipboard] from the
context menu.
The data is copied to the clipboard.
NOTE: Clipboard: An area in main memory (within the PC) used for temporary storage of data.
7/18/2019 FPIA3000 User Manual English August 2006
5-44 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006
9. Graph (Trend-View)
9.1 Introduction
This section explains the [Graph(Trend-View)] screen.
The [Graph(Trend-View)] screen can display average values, mode values, 50% value, lowervalue, upper value, standard deviation, and coefficient of variation can be displayed. This is
effective for looking at the change in each record with the parameters.
For the meanings of parameters, refer to Chapter 16, 5.4 Statistical analysis items.
9.2 Screen composition
Figure 5-47: [Graph(Trend-View)] screen
1 Select the records to display by dragging on the [Record List] screen.
2 Press the [Graph (Trend-View)] tab on the [Record List] screen.
Data (average, mode, 50% value, lower value, upper value, SD (standard deviation), and CV
(coefficient of variation) on the limit particle parameters are displayed for the specified records.
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006 5-49
Figure 5-54: [Import Data]dialog
3 Select the record you want to import and press the [ Import] button. then the measured data
stored in the FPIA-3000 can be imported.
Item Function
Reset Display Reset the sort filter result display or the search setting and return the display tothe default.
Search Search the record including the data you want to search in the selectedrecords.For the searching procedure, refer to 1.6 Search functions.
Select All Select all records in the selected database.
Sort The record information can be rearranged according to the sort items anddisplayed.For the operation procedure, refer to 1.4 Sort functions.
Filter The record which is applicable for the key item can be extracted by setting thekey item.For the operation procedure, refer to 1.5 The filter function.
Import Import the extracted record information to the FPIA-3000.
5-50 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006
10.5 Save
Test data can be made into a new database.
All data can be saved, or only the selected data.
1) All Data
All data displayed on the [Record List] screen can be saved as a new database.
1 Select [Menu] – [File] – [Save] – [All data].
The following dialog screen will be displayed.
Figure 5-55: [Browse For Folder ] dialog
2 Move to the directory in the folder displayed area where you want to create a new database and
press the [Make New Folder ] button. A folder named [New Folder ] will be created. Right
click on the new folder in the folder displayed area and select [Name change] from thedisplayed menu. The folder name changing menu will be displayed. Then, change the name of
the [New Folder ] and press the [OK] button. You can create the new database. When you press
the [Cancel] button, the process will be canceled.
The dialog below appears if data of the same name exists in the same directory. Change the save
location or the file name.
The dialog below is also displayed if there are files in the directory in which you want to create the
database. Empty the directory.
Figure 5-56: Save Confirmation dialog
7/18/2019 FPIA3000 User Manual English August 2006
5-52 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006
10.6 Printing
Prints the data.
1 Select [Menu] – [File] – [Print].
The following dialog screen will be displayed. After placing a check in the checkbox for the item
you want to print and selecting the print format from the list box, press the [Print] button. Thedata for the records selected from the record list are printed according to the print format. Press
the [Cancel] button to return.
Figure 5-59: [Print] dialog
Item Item Functions
Testing Results Record List Select to print the record list.
Analysis Results Select to print the analysis results.
Particle Image Select to print image data.
Graph (Trend-View) Select to print the graph (trend).
Multi Scattergrams Select to print the Multi Scattergrams.
Graph(Overlay-View) Select to print the Graph(Overlay-View).
Frequency Size Frequency Select to print the particle diameter frequency tables.
Shape Frequency Select to print the particle form frequency tables.
User definition Select to print a table defined by the user.
[Analysis]check button
Analysis result data selection Place a checkmark in the check box to print out thecorresponding analysis result in printing.
[FormatSettings] button
The format to be used for printing can be set.
Table 5-18: [Print] dialog items
7/18/2019 FPIA3000 User Manual English August 2006
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006 5-53
10.7 PDF Print
1 Select [Menu] - [File] - [PDF Print].
Figure 5-60: [PDF Print] dialog
The dialog shown in Figure 5-60 appears. Place check-marks at the appropriate items to be printed
and select a print format from the list box, and click the [PDF] button to output the selected records
in the [Record List] screen to the PDF file. Click the [Cancel] button to restore.
NOTE: Click on the [Format Settings] button to set the desired print format. For details, refer toChapter 12, PRINT FORMAT .
CAUTION1. By default, one page of analysis results is printed. The beginning section of the
image display is printed at the bottom of the analysis results print sheet. Toprint the rest of the image, set the number of pages in the format settings. Fordetails, see Chapter 12, PRINT FORMAT .
2. By default, one page of image data is printed. To print the rest of the image, setthe number of pages in the format settings. For details, refer to Chapter 12,PRINT FORMAT .
CAUTIONWhen printing is executed from the FPIA-3000 application, if an equipment erroror the like occurs in the printing, an error message is displayed from the printermanufacturer’s driver installed in Windows. Check the printer manufacturer'suser’s manual and eliminate the error.
5-54 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006
Enter the file name at the place (a) in the PDF Print dialog. It is also possible to select a file in the
edit box and change the file name.
NOTE: The PDF(which is abbreviated to Portable Document Format) which is developed byAdobe Systems is a electronic document to be used to exchange the electronicdocument through the Internet.
Item Item FunctionsTesting Results Record List Select to output the record list to the PDF file.
Analysis Results Select to output the analysis results to the PDF file.
Particle Image Select to output the image data to the PDF file. (Note 1)
Graph (Trend-View) Select to output the Graph (Trend-View) to the PDFfile. (Note 2)
Multi Scattergrams Select to output the Multi Scattergrams to the PDF file.
Graph (Overlay-View)
Select to output the Graph (Overlay-View) to the PDFfile.
Frequency Size Frequency Select to output the particle diameter frequency table to
the PDF file.
Shape Frequency Select to output the particle form frequency table to thePDF file.
User defined Select to output to the PDF file in a format defined byuser.
[Analysis] checkbutton
Analysis result dataselection
Place a checkmark in the check box to print out thecorresponding analysis result in printing as PDF data.
[Format Settings]button
The format to be used for printing can be set.
[Change] button Changing the outputpath
Click the [Change] button to display the Windows File/Folder dialog. Select a desired folder in which file issaved.
Table 5-19: [PDF Print] dialog items
CAUTION• In the default setting, one sheet of the analysis results is output to the PDF file.
In the lower part of the analysis results PDF file, the first part of the imagedisplay will be output to the PDF file. To output the rest of the image to the PDFfile, set the number of prints in the format settings. For details, see Chapter 12,PRINT FORMAT .
• In the default setting, one sheet of the image data is output to the PDF file. To
output the rest of the image to the PDF file, set the number of prints in theformat settings. For details, see Chapter 12, PRINT FORMAT .
CAUTION• When the FPIA-3000 is set to Merge(LPF+HPF) mode, images cannot be
printed even if you try to print the analysis data and particle image data.• For details of Merge(LPF+HPF) mode, refer to Chapter 16, FUNCTIONAL
DESCRIPTION , and for the details of the Merge(LPF+HPF) setting, refer to Appendix C, TABLE OF SYSTEM SETTINGS.
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006 5-55
10.8 CSV export
Data can be exported in CSV file format.
1 Select [Menu] – [File] – [CSV].
The following dialog screen will be displayed.
Figure 5-61: [Export CSV] dialog
NOTE: • CSV format: A file format in which data items are separated by commas “,”. CSV filescan be read by compatible applications (The standard WordPad provided withWindows, MS Office Excel and others).
• Export: The output of data converted for compatibility so that it can be used by otherapplications.
Contents of Output Functions
Record List Check here to export the list of records, then enter the export file name.
Size Frequency Check here to export the particle diameter and frequency, then enter theexport file name. The “Sample Name”, “Sample Number”, and “RecordNumber” are added to the file name.
Shape Frequency Check here to export the particle form frequency table, then enter theexport file name. The “Sample name”, “Sample Number”, and “RecordNumber” are added to the file name.
Scattergram Check here to export the scattergram data, then enter the export filename. The “Sample name”, “Sample No.”, and “Record No.” are addedto the file name.
Output Path The destination directory for the CSV file export can be specified. Followstandard Windows operating procedure to set the path.
Analysis If you press the check bottun, you can output default analysis result andresults of the enhancing 1 to 5 as CSV format.
Table 5-20: [Export CSV] dialog items
7/18/2019 FPIA3000 User Manual English August 2006
5-56 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006
10.9 Transmission to the host
Export all data from the record list, or all selected data, to the host.
1 Select [Menu] – [File] – [Data Transmission].
The following dialog screen will be displayed.
Figure 5-62: [Host Transmission] dialog
For the contents of the information sent, refer to Appendix D, 4. Data format .
Change Set the destination directory for the CSV file export.
Export CSV Export data in CSV file format.
Cancel Close the screen.
Item Functions
Output Select RS-232C or LAN.
Transmit Execute transmission.
Cancel Close the dialog.
Table 5-21: [Host Transmission] dialog items
CAUTIONIf the host is not connected to the data processing section of the FPIA-3000 or thetransmission conditions are not appropriate, if data is transmitted to the host,“Transmission error” is displayed in the [Host Transmission] dialog. Either checkthe transmission condition settings in Chapter 9, SYSTEM SETUP or check thatthe connection cable is connected correctly.
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005
Chapter 6 QUALITY CONTROL
1. Quality control ............................................................................................. 6-11.1 Overview of quality control ....................................................................6-1
1.2 File Settings .......................................................................................... 6-21.3 Adding a quality control file ................................................................... 6-31.4 Deleting a quality control file .................................................................6-51.5 Target setting ........................................................................................ 6-61.6 Auto target............................................................................................. 6-61.7 Deleting a quality control data............................................................... 6-7
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006 6-1
1. Quality control
1.1 Overview of quality control
The state of the instrument can be monitored by regularly measuring parameters, such as the
average diameter of latex particles, and observing time series data.The screen below is displayed via [Maintenance] – [Quality Control]. The target parameter can
be managed as a time series and displayed.
Figure 6-1: [QC Chart] screen
Item Explanation
File Settings Create a new QC data file or remove a QC data file.
Delete Delete QC data.
CSV Save the displayed QC data as CSV data in the file.When the [CSV] button is pressed, QC Save As dialog will be displayed. Inputthe file name and store the CSV file. (The extension should be .csv.)
Print Print the displayed QC data.When the [Print] button is pressed, Windows-based standard print dialog willopen. Perform printer settings if necessary, and print the data.
Close Close the QC chart screen.
Table 6-1: [QC Chart] screen
7/18/2019 FPIA3000 User Manual English August 2006
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006 6-3
1.3 Adding a quality control file
Add new quality control files from the [QC Chart] screen.
1 Register an SOP for particles subject to quality control, then run tests at regular intervals (such
as once every morning). (For details on the SOP registration method, refer to Chapter 8, SOP
SETTINGS .)2 Run [Maintenance] – [Quality Control] and click on the [New file] button to open the [File
Settings] dialog, and then click on the [New file] button.
The following dialog will be displayed.
Figure 6-3: [Add Quality Control File] dialog
Item Explanation
SampleInformation
SOP name Select the SOP name you want to display on the QC chartscreen. (For SOP, refer to CHAPTER 8: SOP SETTINGS".)
Parameter Select the measurement parameter you want to control qualityfrom one of [Analyzed], [Particles], [Limit X axis] and [Limit Y axis]categories. (The actual display names for "Limit X axis" and "LimitY axis" are the limit axes names set with the SOP.
Item Select the measurement parameter you want to control quality.The parameters selected under [Parameters] are displayed in thelist.
QC LimitSettings
Test data When "Read Data from DB" is selected, the past data meeting theSOP name, parameters and items selected in setting the controlsamples is handled as an effective data and create a QC datafile.If you select Stop by Time mode and repeat mesure count isset more than 2, only first data will be imported as control data.When "Do not read Data from DB" is selected, a QC data fileconsisting of SOP name, parameters and items selected insetting the new control samples. In this step, the QC limit settingsselected manually can only be effective.
Table 6-3: [Add Quality Control file] Dialog Items
6-4 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006
3 Enter quality control file information for the dialog items.
The [QC Chart] screen is displayed as shown below.
SampleInformation
LimitSettings
Automaticsetting
The record data which have been measured is extracted as theQC data and QC chart screen will be displayed using the QC limitsettings set with 1 ~ 3SD.Select upper limit:When you want to control quality using the mean value and the
QC limit settings due to SD after reading the measured data,select the upper limit in the QC area.1SD ~3SD are provided.Select lower limit:When you want to control quality using the mean value and theQC limit settings due to SD after reading the measured data,select the lower limit in the QC area.1SD ~3SD are provided.
QC LimitSettings
Manualsetting
Create a QC file based on the settings of the target, upper limitand lower limit.Target:Input the target in display of the measurement parameter youwant to control quality.
Upper limit:Input the upper limit in display of the measurement parameter youwant to control quality.Lower limit:Input the lower limit in display of the measurement parameter youwant to control quality.
Registration The QC file is created.
Cancel Cancel the processing and close the [Add Quality Control file] dialog.
Item Explanation
Table 6-3: [Add Quality Control file] Dialog Items
(D)
(C)
(A)
(B)
Error status display
7/18/2019 FPIA3000 User Manual English August 2006
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006 6-5
Figure 6-4: Explanation of the quality control charts
When the items set for the five parameters(OLensUnit,Light field system,SOP name,paramater and
items) are selected, the following are displayed.
• (A) Quality control graphs.
• (B) Quality control data target values, upper and lower limit values, average, SD, data average,
number of QC error data.• (C) QC data list.
Under (A), the average value is displayed as a green line, the upper and lower limit values as redlines, and the data points as a black line.
Under (B), data exceeding the QC limits are displayed in red spaces within (C), and the number of
QC error data is indicated under the [QC Error ] field of (B).
1.4 Deleting a quality control file
Delete the currently registered quality control data.
1 On the [QC Chart] dialog, select the SOP name, parameters and items for the quality control
chart.
2 Display the quality control data to delete.
3 Press the [DeleteFile] button on the [File Settings] dialog.
The following dialog will be displayed.
Figure 6-5: [Delete QC files] dialog
4 Click on the [OK] button.
The selected quality control data is deleted.
CAUTIONIf you register a quality control file, information will be imported in the qualitycontrol file automatically when you measure a sample using pertinent SOP fromthen on.If quality control range is invalid, a quality control invalid dialog will be shown.Check whether the qualify control sample and the measurement of the FPIA-3000are normal.This dialog box will be closed when [OK] button is pressed.
7/18/2019 FPIA3000 User Manual English August 2006
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005
Chapter 7 TROUBLESHOOTING
1. Error messages ........................................................................................... 7-11.1 Resetting the alarm ............................................................................... 7-1
1.2 Reverting from an error ......................................................................... 7-21.3 Display of error history .......................................................................... 7-3
2. Error message list ....................................................................................... 7-42.1 Error message (in alphabetical order)................................................... 7-42.2 Error code list ........................................................................................ 7-62.3 Error message list by function............................................................... 7-7
3. Error messages and troubleshooting .......................................................... 7-9
7/18/2019 FPIA3000 User Manual English August 2006
The checksum of EEPROM is wrong. ............................................................................. 7-13
The communications to IP board had error. ..................................................................... 7-13
The communications to the temperature control board had error. ................................... 7-13The fan (fluid unit) is out of service. ................................................................................ 7-13
The fan (rear side) is out of service. ................................................................................. 7-13
The fan (stir unit) is out of service. .................................................................................. 7-13
The flash brightness cannot adjust. .................................................................................. 7-10
The flash brightness(DF) cannot adjust............................................................................ 7-10
The flash brightness is abnormal...................................................................................... 7-10
7/18/2019 FPIA3000 User Manual English August 2006
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006 7-5
The flash has abnormal brightness....................................................................................7-10
The gas sensor of Fluid unit is out of service. ..................................................................7-14
The pressure decrease abnormality ...................................................................................7-11
The rejection liquid Chamber cannot exhaust...................................................................7-11
The sampling cover Error. ................................................................................................7-12
Testing unit communication error. (command transmission error) ..................................7-14Testing unit communication error. (response time-out)....................................................7-14
Testing unit communication error. (USB protocol error) .................................................7-14
The testing unit is not connected.......................................................................................7-14
The stir motor cannot adjust..............................................................................................7-10
The stir motor had abnormal rotation. ................................................................................7-9
The temperature control board is not connect...................................................................7-13
The temperature of the sheath liquid is out of range.........................................................7-12
The temperature of the testing unit is out of range. ..........................................................7-12
The temperature of the thermo fin is out of range. ...........................................................7-12
The Temperature sensor of the detector is not connected.................................................7-13
The Temperature sensor of the sheath liquid is not connected. ........................................7-13The Temperature sensor of the testing unit is not connected............................................7-13
The Temperature sensor of the thermo fin is not connected.............................................7-13
7/18/2019 FPIA3000 User Manual English August 2006
214010 Sample Syringe Error. .......................................................................................... 7-9242100 The stir motor had abnormal rotation................................................................... 7-9
242101 The stir motor cannot adjust. .............................................................................. 7-10
421520 The sampling cover Error................................................................................... 7-12429100 Solvent is Leaked from fluid unit....................................................................... 7-14
429101 Solvent is Leaked from detector......................................................................... 7-14
429110 The gas sensor of Fluid unit is out of service. .................................................... 7-14
429111 Gas sensor of detector part is out of service....................................................... 7-14
429120 The fan (rear side) is out of service.................................................................... 7-13
429121 The fan (fluid unit) is out of service................................................................... 7-13
429122 The fan (stir unit) is out of service. .................................................................... 7-13
431020 The checksum of EEPROM is wrong. ............................................................... 7-13
432042 Data file cannot open.......................................................................................... 7-14
441092 Testing unit communication error. (response time-out) ..................................... 7-14441093 Testing unit communication error. (command transmission error).................... 7-14
451064 The communications to the temperature control board had error. ..................... 7-13
451065 The temperature control board is not connect. ................................................... 7-13
451081 IP board is not connect. ...................................................................................... 7-13
451082 The communications to IP board had error. ....................................................... 7-13
466110 The automatic focusing Error............................................................................. 7-10
486020 The testing unit is not connected........................................................................ 7-14
486021 Testing unit communication error. (USB protocol error)................................... 7-14
7/18/2019 FPIA3000 User Manual English August 2006
The stir motor had abnormal rotation. ...............................................................................7-9
The stir motor cannot adjust. ............................................................................................7-10Sheath Syringe Error. .........................................................................................................7-9
The automatic focusing Error. .........................................................................................7-10
7-8 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006
[Host-Related]Testing unit communication error. (USB protocol error)................................................. 7-14
The testing unit is not connected. ..................................................................................... 7-14
Data file cannot open........................................................................................................ 7-14
[Gas sensor related]Solvent is Leaked from detector....................................................................................... 7-14Solvent is Leaked from fluid unit..................................................................................... 7-14
Gas sensor of detector part is out of service..................................................................... 7-14
The gas sensor of Fluid unit is out of service................................................................... 7-14
[Other]The flash has abnormal brightness. ................................................................................. 7-10
The flash brightness cannot adjust. ................................................................................. 7-10
The flash brightness(DF) cannot adjust. .......................................................................... 7-10
The flash brightness is abnormal. .................................................................................... 7-10
7/18/2019 FPIA3000 User Manual English August 2006
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005 7-11
Error code 141000
Error message The rejection liquid Chamber cannot exhaust.
Cause 1) The tube for discarding waste fluid is bent.
Solution 1) Unbend the tube, then revert from the error.
Error code 113022
Error message 0.22MPa pressure Error.
Cause 1) No pressure supplied from the pneumatic unit.2) The tube for supplying pressure from the pneumatic unit is bent.
Solution 1) Adjust the pressure from the pneumatic unit, and revert from the error. (Refer to Chapter 14,1. Adjusting the pressure of the pneumatic unit .)
2) Unbend the tube, then revert from the error.
Error code 113010
Error message Cleaning Pressure Error.
Cause 1) No pressure supplied from the pneumatic unit.2) The tube for supplying pressure from the pneumatic unit is bent.3) Adjustment of the pressure inside the testing unit was wrong.
Solution 1) Adjust the pressure from the pneumatic unit, and revert from the error. (Refer to Chapter 14,1. Adjusting the pressure of the pneumatic unit .)
2) Unbend the tube, then revert from the error.3) Readjust the pressure inside the testing unit, then revert from the error. (Refer to Chapter 14,
2.3 Adjustment of rinse pressure.)
Error code 119010
Error message The pressure decrease abnormality
Cause 1) No pressure supplied from the pneumatic unit.2) The tube for supplying pressure from the pneumatic unit is bent.3) Adjustment of the pressure inside the testing unit was wrong.
Solution 1) Adjust the pressure from the pneumatic unit, and revert from the error. (Refer to Chapter 14,1. Adjusting the pressure of the pneumatic unit .)
2) Unbend the tube, then revert from the error.3) See if the testing-unit pressure matches that for the current sheath liquid. If not, adjust it, then
revert from the error.
Error code 114005
Error message -0.053MPa pressure Error.
Cause 1) No vacuum supplied from the vacuum unit.2) The tube for supplying vaccum from the vacuum unit is bent.3) Adjustment of the pressure inside the testing unit was wrong.
Solution 1) Readjust the pressure inside the testing unit, then revert from the error. (Refer to Chapter 14,2.4 Adjusting the -0.05 MPa Pressure.)
2) Unbend the tube, then revert from the error.3) Readjust the pressure inside the testing unit, then revert from the error. (Refer to Chapter 14,
7-12 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005
Error code 121030
Error message The temperature of the testing unit is out of range.
Cause 1) Ambient temperature has left the 15-30°C range.
Solution 1) Adjust the ambient temperature to 15-30°C.
Error code 123019
Error message The temperature of the sheath liquid is out of range.
Cause 1) Sheath liquid temperature has deviated from the set value.
Solution 1) The error is cancelled automatically when the sheath liquid temperature enters the set range.If the error persists, contact your Sysmex service representative for an inspection.
Error code 125520
Error message The temperature of the thermo fin is out of range.
Cause 1) Temperature near the radiator f ins has deviated from the set value.Solution 1) Turn off the instrument power, then contact your Sysmex service representative for an
inspection.
Error code 351110
Error message Detection time-out
Cause 1) A communication error occurred between the testing unit and the data processing unit.
Solution 1) Check that the USB cable is connected, then revert from the error.
Error code 351120
Error message Image analysis time-out
Cause 1) Image analysis processing did not complete within the regulation time.
Solution 1) Revert from the error.
Error code 421520
Error message The sampling cover Error.
Cause 1) The opening/closing action of the sample mixing chamber cover did not function properly.
Solution 1) Revert from the error.
7/18/2019 FPIA3000 User Manual English August 2006
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005 8-1
1. SOP settings
1.1 Explanation of SOP
SOP is the abbreviation for Standard Operation Procedure. The measurement conditions, analysis
conditions, hardware settings, and other setting information related to measurement are managedall together in the database in the analysis PC, making it easy to manage user measurement
conditions. FPIA-3000 has functions for registering, deleting, editing, and using SOPs.
• SOPs can be selected in the measurement and analysis condition dialogs and used for
measurement.
• On the analysis results screen, new SOPs can be registered and SOPs selected to use for data re-
analysis.
• You can do the followings with [Settings] – [SOP]; register, edit, delete, export and import
SOPs, and change the names of them.
1.2 Using SOPsAlready registered SOPs can be selected for use in measurement from the measurement and
analysis condition dialogs. For the concrete usage procedure, refer to Chapter 4, 3.2 SOP setting .
On the analysis results screen, existing SOPs can be selected to use for data re-analysis. For the
concrete usage procedure, refer to Chapter 5, 2.5 Reanalyze.
1.3 Registering SOPs
On the [SOP Setting] dialog, new SOPs can be registered, and new SOPs can be registered on the
analysis results screen. For the concrete usage procedure, refer to Chapter 5, 2.5 Reanalyze.
How to register the SOP using [Settings] - [SOP] is explained here.Use the Up and Down buttons to select the item from the SOP list you want to check in the SOP
settings dialog, and press the [Registration] button.
Figure 8-1: [new SOP registration] dialog
Input the SOP name in the SOP name input area and press the [OK] button.When a message which says “Save New SOP, Are you sure ?” is displayed, press the [OK] button.
The new entered SOP name is registered and displayed in the SOP list.
To edit the SOP, refer to 1.5 Editing and deleting SOPs.
New SOP Registration
7/18/2019 FPIA3000 User Manual English August 2006
8-2 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006
1.4 Changing SOP's name
Select the SOP you want to change its name from the SOP list in the SOP Settings dialog. When
you press the [Name Change] button, the following dialog will be displayed.
Figure 8-2: [SOP name change] dialog
Change the SOP name indicated in the SOP name change area and press the [OK] button.
When a message which says "Replace SOP. Are you sure ?" appears, press the [OK] button.
The SOP name is changed.
You can confirm the changed SOP name from the SOP list in the SOP Settings dialog.
1.5 Editing and deleting SOPs
You can edit and delete SOPs with [Settings] – [SOP].
Figure 8-1 is displayed via [Settings] – [SOP].
It can be used to edit and delete SOPs.
(1) Editing SOPs
A list of the registered SOPs is displayed in the SOP settings window. Left click the name of theSOP you want to edit or check in the SOP list box. The detailed parameters of the selected SOP are
displayed in the right list box in the SOP settings window.
To edit SOP parameters, left click the appropriate value field in the SOP settings window. This
enables you to select an item for the information or to edit it.
After selecting an item for the stipulated information or editing it, press the [Update] button. The
SOP contents are changed. When the LensUnit changing combo box in the SOP settings window
is selected and the magnification of the lens unit is changed, the corresponding SOP is displayed in
the SOP list box. Light field system (Bright field/Dark field) can be switched only when the
standard magnification (10X) is selected.
CAUTIONFollowing SOP name cannot be changed.AUTOFOCUS, AUTOFOCUSMSR, CALIBHPF and CALIBLPF
NOTE: • For details of the Objective Lens, refer to the Appendix H, HIGH/LOW
MAGNIFICATION LENS UNIT MANUAL (OPTION).• For details of the Light field system, refer to the Appendix J, FPIA-3000 DARK
FIELD UNIT MANUAL.
SOP name change area
7/18/2019 FPIA3000 User Manual English August 2006
8-4 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005
1.6 SOP Import / Export
(1) Export SOP
You can export the selected SOP or the all registered SOP information in the file. (The file
name should be **.sop.)
Use the radio button at the lower of the Import button and select All Data or Selected Data.When the [Export] button is pressed, the following dialog will be displayed.
Figure 8-4: [Save As..] dialog
Select the folder you want to save or create a new folder here. Input the SOP save file name in thefile name input area and press the [Save] button. The SOP information will be exported.
File name input area
7/18/2019 FPIA3000 User Manual English August 2006
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006 8-5
(2) Import SOP
Press [Import] button. followg Figure 8-5 SOP list dialog will be shown.
In this dialog, click SOP name, highlight the SOP names by dragging the mouse over it with
pressing left cursor or select [Select All] button. Press [Import] button if you select SOP.
Figure 8-5: [SOP list] dialog
Reread the imported SOP and additionally register it in the present SOP information.
If the registered SOP name and the SOP name to be exported are the same or if the update date
or content is different form the file information to be exported, "Same name already exists.
Please input another name." dialog will be displayed.
When you change the import SOP name here and press the [OK] button, the SOP will be
imported as another name.
The exported SOP name and the registered SOP name will be displayed in the SOP list.
NOTE: When the contents of the registered SOP and the SOP to be exported are the sameeven if the both names are the same and the update dates are different, SOP will not beimported.
7/18/2019 FPIA3000 User Manual English August 2006
8-6 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006
Figure 8-6 explains the overall flow of the FPIA-3000 analysis using the SOP.
Figure 8-6: Relational chart for SOP settings
Analysis conditions setting screen (SOP settings when analyzing)
This screen can be used to set the SOP conditions by the following twomethods.1) Select the existing SOP conditions and carry out analysis2) Select the existing SOP conditions and revise them to carry out
analysisThe revised SOP settings will not be registered if you select 2).
When reanalyzing
(after analysis)
SOP selection and revision screenList boxes that include revisedconditions cannot be selected.
Reanalysis screen
When reanalyzing,1) Perform reanalysis using the existing SOP
2) Register a new SOP based on the existing SOPconditions to carry out reanalysis
Setting SOPs and reanalysis can be carried out with thetwo methods above.
Reanalysis conditions setting screen
Select the existing SOP and editparameters. When the parameters arechanged, the list cannot be selected.
SOP settings
The screen can be used to register, edit ordelete the existing SOPs.
7/18/2019 FPIA3000 User Manual English August 2006
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005
Chapter 9 SYSTEM SETUP
1. System setup............................................................................................... 9-11.1 Setting menu items ............................................................................... 9-1
1.2 System settings..................................................................................... 9-1
2. Sheath fluid settings (Only for the FPIA-3000S) ......................................... 9-52.1 Effects by sheath physical characteristics.............................................9-52.2 Sheath fluid specific hardware settings................................................. 9-6
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005 9-5
2. Sheath fluid settings (Only for the FPIA-3000S)
2.1 Effects by sheath physical characteristics
Particle images are captured with the most appropriate focusing conditions by focusing the
objective lens on the flat sample layer that is formed in the flow cell by a sheath flow.This flat sheath formation is highly influenced by the sheath fluid flow and the physical
characteristics of the sheath fluid, such as viscosity and specific gravity.
When a high viscosity fluid, such as 2-propernol, is used as a sheath fluid instead of PARTICLE
SHEATH, for example, the sample flow is not thin enough in the flow cell and poorly focused
images will be obtained. In this case, the sheath fluid flow is increased compared with that of
PARTICLE SHEATH, and sample flow is given with a bigger shearing stress to form a thinner flat
sheath flow as similar to that with PARTICLE SHEATH.
Accordingly, when a lower viscosity fluid, such as methanol, is used as a sheath fluid, a sample is
widely spread and the flat sheath formation becomes unstable. In this case, the sheath fluid flow is
decreased compared with that of PARTICLE SHEATH, and sample flow is given with a smaller
shearing stress to form a stable flat sheath flow as similar to that with PARTICLE SHEATH.
It is necessary to control the fluid flow of the sample and sheath fluid in accordance with the
physical characteristics of the sheath fluid to be used.
FPIA-3000S employs 2 sets of the syringes driven by a stepper motor to allow a control of the
sample flow and the sheath flow individually and to form the most suitable flat sheath.
CAUTION
• Depending on the physical characteristics (viscosity and specific gravity)
of the organic solvent to be used, there is a potential that the mostsuitable flat sheath formation may not be obtained in the tolerable rangeof the syringe speed adjustment by the FPIA-3000S. In this case,
reselect the dispersion medium and the sheath fluid.
7/18/2019 FPIA3000 User Manual English August 2006
9-6 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005
2.2 Sheath fluid specific hardware settings
When any organic solvent other than the PARTICLE SHEATH is used as a sheath fluid, change
the following hardware setting values according to the physical characteristics (viscosity and
specific gravity) of the sheath fluid.
2.2.1. Sheath syringe speed setting value
Based on the sheath syringe speed Vp when PARTICLE SHEATH is used, the sheath syringe speed
Vs when an organic solvent is used can be calculated using with the viscosity and the specific
gravity of the PARTICLE SHEATH and the organic solvent.
Set Items Descriptions
Sheath formation syringe speed Adjusts the sheath flow in the flow cell by changing the pulse persecond (PPS) of the stepper motor which drives the sheathformation syringe.
Sample aspiration syringe speed Adjusts the sample suspension flow to feed from the samplemixing chamber to the flow cell by changing the pulse per second(PPS) of the stepper motor which drives the sample injectionsyringe.
Rinse fluid pressure Adjusts the rinse sheath flow supplied to the sample mixingchamber and the flow cell by changing the set pressure (MPa) ofthe rinse pressure regulator.
Gas sensor level Adjusts the sensitivity of the gas sensor that detects the leakageof the organic solvent.
Table 9-3: Hardware setting items to adjust for the sheath fluid characteristics
CAUTION• FPIA-3000S controls the temperature of the optical detector unit and the
sheath fluid at 25ºC. Use the physical characteristics (viscosity andspecific gravity) of the sheath fluid at 25ºC in the following calculations of
the sheath formation syringe speed and the rinse pressure.
Sheath syringe speed Vs is calculated as follows.
Vs = Vp * (µs/ ρs)/(µp/ ρp)
= Vp * νs/ νp
Here, µs is the viscosity of the applied organic solvent at 25ºC (mPa•s).µp is the viscosity of the PARTICLE SHEATH at 25ºC (mPa•s).
ρs is the specific gravity of the organic solvent at 25ºC (no unit).
ρp is the specific gravity of the PARTICLE SHEATH at 25ºC (no unit).
νs is the kinematic viscosity of the organic solvent at 25ºC (m2 /s).
νp is the kinematic viscosity of the PARTICLE SHEATH at 25ºC (m2 /s).
7/18/2019 FPIA3000 User Manual English August 2006
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005 9-7
An example is shown below to calculate the sheath forming syringe speed Vs when using the
toluene as a sheath fluid.
Therefore, Vs = Vp * (µs/ ρs)/(µp/ ρp)
= 600 *(0.553/0.862)/(0.890/0.997)
= 431 pps
Item Set value and physical value
Vp: Sheath forming syringe speedwhen using the PARTICLE SHEATH
600 pps in the HPF mode
600 pps in the LPF mode
µp: Viscosity of the PARTICLE SHEATH at 25ºC 0.890 mPa•s
ρp: Specific gravity of the PARTICLE SHEATH at 25ºC 0.997
Table 9-4: Physical characteristics of the PARTICLE SHEATH at 25ºC
Item Set value and physical value
µs: Viscosity of the toluene at 25ºC 0.553 mPa•s
ρs: Specific gravity of the toluene at 25ºC 0.862
Table 9-5: Physical characteristics of the toluene at 25ºC
NOTE: • The set range of the sheath forming speed Vs in the FPIA-3000S is shown inthe following.
Depending on the viscosity and specific gravity of the organic solvent to beused as a sheath fluid, the sheath forming syringe speed Vs may be calculatedas an outside value of the set range stated above.If Vs is calculated below the lower limit value, set at the lower limit value. If the
Vs is calculated over the upper limit value, set at the upper limit value.
In this case, particle images may not be well focused or the detected number
of particles may be slightly lower than the actual, since the flat sheath formingconditions are not the most appropriate.
350 pps Vs 900 pps≤ ≤
If Vs calculated < 350 pps, then set value = 350 pps.If Vs calculated > 900 pps, then set value = 900 pps.
7/18/2019 FPIA3000 User Manual English August 2006
9-8 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005
2.2.2. Sample aspiration syringe speed setting value
The sample aspiration syringe speed Us when an organic solvent is used as a sheath fluid is set to
the common value as the sample aspiration syringe speed Up when the PARTICLE SHEATH is
used.
The sample aspiration syringe speed Us = Up = 400 pps
NOTE: • When the sample aspiration syringe speed is set as Us = Up = 400 pps as
stated above, the number of detected particles may become very low or veryhigh depending on the viscosity and specific gravity of the organic solvent.In this case, prepare a known concentration sample, and readjust the sample
aspiration syringe speed Us by using the analysis results of the known sampleto adjust the number of particles, as follows.
1) Procedures to make a known concentration sample
Using the focus adjustment particle (melamine resign MF-R-2.0) described in Chapter4, prepare a sample with a known concentration.(1) Using the following formula, measure the Mg weight of the focus adjustment
particle (melamine resign particle MF-R-2.0).
Here, ρ: particle density (= 1.51 g/cm3)D: particle diameter (= 2 µm)V: sample volume to be preparedCONC: concentration of the sample to be prepared
For example, when 100 mL of the sample of which concentration is approximately30000 particles per µL is prepared, M = approx. 19 mg.
(2) Mono-disperse the Mg of the focus adjustment particle (melamine resign particleMF-R-2.0) in the specific volume of the organic solvent. To accelerate dispersionof the particles, add an appropriate amount of the dispersant according to theorganic solvent to be used, and use the ultrasonic dispersing devices asrequired.
2) Re-adjustment of the sample aspiration syringe speedAfter the explained settings for the sheath fluid are entered in the analysis software,run the known concentration sample prepared in the step 1) above.If analyzed sample concentration is lower than that calculated, heighten the settingvalue of the sample aspiration syringe speed.If analyzed sample concentration is higher than that calculated, lower the setting valueof the sample aspiration syringe speed.
M = ρ (πD3 /6) V CONC
7/18/2019 FPIA3000 User Manual English August 2006
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005 9-9
2.2.3. Setting value for the rinse pressure
In each run cycle, FPIA-3000S automatically rinses hydraulic parts, such as the sample mixing
chamber and flow cell, in which the sample passes through, using the sheath fluid. The flow of the
rinse fluid is affected by the viscosity of the applied sheath fluid.
Based on the rinse pressure regulator setting value Pp when using the PARTICLE SHEATH, the
rinse pressure Ps for the applied organic solvent is calculated by using the viscosities of thePARTICLE SHEATH and the applied organic solvent.
An example is shown below to calculate the rinse pressure Pp when using the toluene as a sheath
fluid.
Ps = Pp * (µs/ µp)
= 600 *(0.553/0.890)
= 0.061 MPa
Calculation of the rinse pressure Ps:
Ps = Pp (µs/ µp)
Here, µs is the viscosity of the applied organic solvent at 25ºC (mPa•s).
µp is the viscosity of the PARTICLE SHEATH at 25ºC (mPa•s).
Item Set value and physical value
Pp: Rinse pressure when using the PARTICLE SHEATH 0.098 MPa
Table 9-6: Rinse pressure when using the PARTICLE SHEATH
NOTE: • The set range of the rinse pressure Ps in the FPIA-3000S is shown below.
Depending on the viscosity of the organic solvent to be used as a sheath fluid,the rinse pressure Ps may be calculated as an outside value of the set rangestated above.If the calculated Vs exceeded the upper limit of the set range, set to the upper
limit value.
Ps ≤ 0.098 MPa
When the calculated Ps > 0.098 MPa, Ps set value = 0.098 MPa.
7/18/2019 FPIA3000 User Manual English August 2006
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006 9-11
2) Additional registration of a sheath fluid
When the [Addition] button shown in Figure 9-3 is pressed, a dialog shown in Figure 9-4 will
be displayed and an organic solvent can be added as a sheath fluid candidate. Type in the
sheath fluid name in the edit box, and press the [Registration] button. A new sheath fluid of
which name is as typed in will be registered.
Figure 9-4: Adding a new sheath fluid (an example of toluene)
3) Changing the added sheath fluid hardware settings:
When the [Registration] button is pressed as shown in Figure 9-4, a dialog will be displayed
with the sheath fluid name added and its hardware setting items. Change the values as
calculated accordingly by referring to 2.2.1 through 2.2.4 in this Chapter.
Figure 9-5: Changing the hardware settings (an example of toluene)
Click the [Apply] button shown in Figure 9-5, and the added sheath fluid settings are registered in
the FPIA-3000S analysis software and are displayed when the system is starting up or in the sheath
fluid selection menu when the sheath fluid is replaced.
CAUTION• Immediately after a new sheath fluid is registered, setting values of the
PARTICLE SHEATH are entered as default values. Make sure to
change the setting values accordingly to the applied sheath fluid.
NOTE: • In the same procedures described in 3) above, it is also possible to select aregistered sheath fluid and to change setting values for a new sheath fluid.
7/18/2019 FPIA3000 User Manual English August 2006
11-4 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005
Item Item Functions
SizeHistgram
Frequency graph Set the Frequency graph display colors.
Cumulative graph Set the Cumulative graph display colors.
Frequency scale Set the frequency scale automatically or manually. If youremove the check from Auto, the scale is set manually. At this
time, the “Fix to **%” input item is used as the upper limit forthe scale.
Frequency scale- Main Scale Pitch
Sets the main scale pitch for the frequency scale.
Frequency scale- Number of Sub-Scales
Sets the number of auxiliary lines between the main scale ofthe frequency scale.
Parameter scale- Number of Sub-Scales
Sets the number of auxiliary lines between the main scale ofthe particle distribution axis.
ShapeHistgram
Frequency graph Set the Frequency graph display colors.
Cumulative graph Set the Cumulative graph display colors.
Frequency scale Set the frequency scale automatically or manually. If youremove the check from Auto, the scale is set manually. At thistime, the “Fix to **%” input item is used as the upper limit forthe scale.
Frequency scale- Main Scale Pitch
Sets the main scale pitch for the frequency scale.
Frequency scale- Number of Sub-Scales
Sets the number of auxiliary lines between the main scale ofthe particle form distribution axis.
Parameter scale- Main Scale Pitch
Set the main scale value for the particle form axis.
Parameter scale- Number of Sub-Scales
Sets the number of auxiliary lines between the main scale ofthe particle form distribution.
Scattergram Upper Scattergram
number
Set the dot display colors for the upper limit and its above limit.
Middle RangeFrequency
Set the dot display color for the midpoint width, which iscalculated from the set upper limit and lower limit widths.
Lower Scattergramnumber
Set the dot display colors for the lower limit and its below limit.
Marked Data Set the color used when a par ticle has been marked asselected within the image display.
ForeColor Set the text color for the cell size distribution, particle form andscattergram.
BackColor Set the background color for the cell size distribution, particleform and scattergram.
Out of Selection Set the color for the area outside the limits on the Analysisresults screen.
Default Set the settings to the factory defaults.
OK Carry out the settings.
Cancel Cancel the settings.
Table 11-3: [Result Graph] dialog
7/18/2019 FPIA3000 User Manual English August 2006
12-2 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005
This [Print Format Settings] dialog can also be displayed by selecting [Menu] – [File] –
[Print] – [Print Format Settings].
1.1 Importing File
When [File] - [Import] menu is selected from the print format setting dialog, the following dialog
will be displayed.
Figure 12-2: [Print format selection] dialog
Select the file you want to use from the file name selection area in the print format selection dialog
and press the [Open] button.
The relevant file format is imported.
NOTE: Testing result (Standard) display is the default setting of the display format in theanalysis result tab. However a format which can be printed with information of the
cumulative frequency result is also installed and can be used.
7/18/2019 FPIA3000 User Manual English August 2006
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005 12-5
1.4 New format creation and editing
Follow the procedure below to edit a print format as a new format.
1 Press the [Analysis Results] tab.
2 When creating a new print format, press the [New] button.
The following dialog will be displayed.
Figure 12-7: [Editting List Print Format] dialog
Item Explanation
Components for graphic(icons)
Left click the component icon to place, then drag it into the print formatarea.
Paper size Select the size (height and width) of the paper as A4/A3/B4/B5/letter.
Preview Display a preview of the created print layout.
Del Specify a certain placed component and delete it.
DelAll Delete all placed components.
Save New Save the created and edited print layout.
Replace Overwrite the created and edited print layout.
Cancel Close the print layout without saving.
Bring to Front The selected parts will be displayed in the front side by pressing [Bringto Front] after selecting the parts arranged in the print form in the formdesign dialog.
Send to Back The selected parts will be displayed in the back side by pressing [Sendto Back] after selecting the parts arranged in the print form in the formdesign dialog.
Table 12-2: [Editting List Print Format] dialog items
Components forgraphic icons
Print form
7/18/2019 FPIA3000 User Manual English August 2006
12-6 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005
3 When creating an existing print format, press the [Edit] button.
Enable editing of a print layout.
4 To edit, select the part you want to print by clicking its icon on the left, move it to the desired
print position by moving the mouse while holding its left button down (dragging), decide the
size, then release. The print image of the selected part is displayed on the print form. The data
displayed at the part is the data selected in the record list.The grid (one grid is approx. 1.3 mm (75 twip)) will be displayed in the print form. You can
move the parts with the mouse by the unit of the grid.
5 With the arrow parts icon selected, if you select the part, you can move the part and change its size.
6 Also, you can change the print method by right clicking the mouse cursor on the part. (This
function is only for parts with setting values.)
7 There are two types of drawing parts. Which you can use depends on the tab selected before
starting editing.
• Form type: Print form straddling multiple records
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005 12-9
Character (Label): Property Setting
When you press the character part in the print form and right click on the part, the following dialog
will be displayed.
Figure 12-9: [Label: Property Setting] dialog
Property item Functions
Input Characters Input characters to be displayed.
Character Alignment Align the input characters to Left, Right or Center.
Font Select the fonts registered in the Windows OS.
Bold Select Apply or Not Apply for the bold display.
Italic Select Apply or Not Apply for the italic display.
Font size Set the font size between 6 and 72.
Underline Select Apply or Not Apply for the underline.
Fore Color Set the fore color.Double click on the fore color area to display the color settingdialog.
Top Taking the upper left of the print form for origin, set the verticaldistance from the form origin to the upper left of the part.(Hereinafter referred to as Top.)
Left Taking the upper left of the print form for origin, set the horizontaldistance from the form origin to the upper left of the part.(Hereinafter referred to as Left.)
Height Set the vertical length of the part.
(Hereinafter referred to as Height.)
Width Set the horizontal length of the part.(Hereinafter referred to as Width.)
Table 12-6: Label: Property Setting Dialog Items
7/18/2019 FPIA3000 User Manual English August 2006
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005 12-11
Picture: Property Settings
When you place the picture part in the print form and right click on the part, the following dialog
will be displayed.
Figure 12-11: [Picture: Property Settings] dialog
Property item Functions
Picture file Select the Picture file.When the picture fie area double clicked on, the picture filesettings dialog will be displayed.The file properties you can select are BMP, JPEG and ICO files.
Top Set the Top information on the picture part.
Left Set the Left information on the picture part.
Height Set the Height information on the picture part.
Width Set the Width information on the picture part.
Index For the document type (this type is not provided for the cut papertype), the index of the part placed in the print form is used formatching with the testing/analysis parameter part.When the part is placed in the position corresponding to the indexwhich is the same in the Scattergram property and test/analysisparameter, the data matching property is maintained.
Top Set the Top information on the Scattergram part.
Left Set the Left information on the Scattergram part.
Height Set the Height information on the Scattergram part.
Width Set the Width information on the Scattergram part.
ReAnalyzed Select Display or Not Display on the part.
ReAnalysis Select Display or Not Display on the part.
Measurer name Select Display or Not Display on the part.
ReAnalyzed by Select Display or Not Display on the part.
Sample name Select Display or Not Display on the part.
Sample number Select Display or Not Display on the part.
Sample type Select Display or Not Display on the part.
Comment Select Display or Not Display on the part.
SOP Select Display or Not Display on the part.
Power Field Select Display or Not Display on the part.
Count method Select Display or Not Display on the part.
Sheath Liquid Select Display or Not Display on the part.
Field Lens Select Display or Not Display on the part.
Light field system Select Display or Not Display on the part.Selection data Save Select Display or Not Display on the part.
Merging data Select Display or Not Display on the part.
Index For the document type (this type is not provided for the cut papertype), the index of the part placed in the print form is used formatching with the testing/analysis parameter part.For details, refer to "Scattergram: Property Settings".
Analyzed Parameter Display Select the information displayed under the particle image fromDisplay or Not Display of No Parameter, X axis parameter orY axis parameter
Display Size Select particle image size from 2X, 4X, 8X or 10X.
Sort Select the particle image display from the order of frame, X axisascending frame, descending order of X axis, Y axis ascendingorder Descending order of Y axis.
Number of max. print pages Set the number of printing pages.When 0 is set to, all images can be printed.
Top Set the Top information on the particle image part.
Left Set the Left information on the particle image part.
Height Set the Height information on the particle image part.
Width Set the Width information on the particle image part.
Boundary line Select the particle image boundary line from Apply or Not Apply.
Mark Display Select from All particles, Marked particles, or No MarkedParticles. Mark particles image, No mark particles image of theparticles image list and all particles image will be shown.
Display Data Selection Select frequency table display from Diameter / Particle frequencydistribution.
Width of display: Range Print the item list of the frequency distr ibution range, numberswithin the range, frequency in the range (%) and cumulation inthe range (%).Set the width of each raw.
Width of display: Number
Width of display: Frequency
Width of display: Cumulation
Top Set the Top information on the frequency table part.Left Set the Left information on the frequency table part.
Height Set the Height information on the frequency table part.
Width Set the Width information on the frequency table part.
Table 12-18: Frequency Table Property Settings Dialog Items
7/18/2019 FPIA3000 User Manual English August 2006
2 Enter the password and click on the [OK] button.The password is changed.
NOTE: Logon is required when the FPIA-3000/3000S boots up.
Menu Functions
Change password Change the password for the logged-on user.
User Registration Make settings for accounts and logon.
Table 13-1: Submenu items
Item Functions
New password Enter the password to change. The input characters are displayed asasterisks (*). Alphanumeric characters can be entered. The number ofcharacters which can be used is within the range set in [Edit Password/Account] dialog. The input distinguishes between upper case and lowercase characters.
New password (Retype) Re-enter the same password entered under [New password].
Table 13-2: [Change Password] dialog items
7/18/2019 FPIA3000 User Manual English August 2006
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005 13-5
2 Set the above items and click on the [OK] button.
The account is added.
Item Entry range Explanation
Logon ID Any Enter the logon ID. The input distinguishes between uppercase and lower case characters.
User Name Any Enter the user name. User names cannot be altered oncethey have been registered.
Access Group Set account rights. Access groups set under group settingscan be selected.
Comments Any Enter a comment.
Password Set the password. Alphanumeric characters can beentered. The input characters are displayed as asterisks(*). The number of characters which can be used is withinthe range set for the password account. The inputdistinguishes between upper case and lower casecharacters.
Password Again Re-enter the same password entered under [Password].
Default DB Any C:\ProgramFiles\FPIA-3000\name sample.rbk will be set
as default DB name where above string "name" is youruser name.If you select [OK] button, a message dialog which states"This file does not exist, create the file?" will be shownwhen default DB does not exist. New default DB will becreated if you select [OK] button here.
Account Indefinite Disable the account without deleting the user settings.
Lockout Duration The user can log in until the date set for the account. Anexpiration date warning dialog will be displayed at logon for14 days before expiration. Once the expiration date passes,the user will not be able to log on to the accountconcerned.
Invalid Disable the account and delete the user settings.
PasswordExpiring Date
Indefinite No expiration date is set for the password.
Valid With Timeout An expiration date is set for the password. It is calculatedas a number of days from the date of registration orpassword alteration. An expiration date warning dialog willbe displayed on logon for 14 days before expiration. Oncethe expiration date passes, the user will not be able to logon using the password concerned.
Table 13-5: [User Registration] dialog items
7/18/2019 FPIA3000 User Manual English August 2006
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005 13-7
2 Set the above items and click on the [OK] button.
The account is edited.
Item Entry range Explanation
Logon ID Any Enter the logon ID. The input distinguishes between uppercase and lower case characters.
User Name Any Enter the user name. User names cannot be altered oncethey have been registered.
Access Group Set account rights. Access groups set under group settingscan be selected.
Comments Any Enter a comment.
Password Set the password. Alphanumeric characters can beentered. The input characters are displayed as asterisks(*). The number of characters which can be used is withinthe range set for the password account. The inputdistinguishes between upper case and lower casecharacters.
Password Again Re-enter the same password entered under [Password].
Default DB Any Set the database to be used by the user. However, there
must be a database in the set directory.
Account Indefinite No expiration date is set for the account.
Lockout Duration The user can log in until the date set for the account. Anexpiration date warning dialog will be displayed on logonfor 14 days before expiration. Once the expiration datepasses, the user will not be able to log on to the accountconcerned.
Invalid Disable the account and delete the user settings.
PasswordExpiring Date
Indefinite No expiration date is set for the password.
Valid With Timeout An expiration date is set for the password. It is calculatedas a number of days from the date of registration or
password alteration. An expiration date warning dialog willbe displayed on logon for 14 days before expiration. Oncethe expiration date passes, the user will not be able to logon using the password concerned.
Reject Counts Rejected (Times) Shows the number of times in the login dialog that the userID or password was not input correctly so login was notpossible.
[Clear ] button Pressing this button clears the reject count.
Table 13-6: [User Information Edit] dialog items
7/18/2019 FPIA3000 User Manual English August 2006
13-10 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005
Time-Controled Lockout
The system can be locked out if there is no operation of the FPIA-3000 within a certain period.
1 Select [ON] under [Time-Controled Lockout] on the [Password/account] screen.
2 Set how many minutes should elapse from the last operation before the lockout acts.
The automatic lockout is set.
The following dialog will be displayed when automatic lockout acts.
Figure 13-9: [Lockout] dialog
1 Enter the password and click on the [OK] button.
The automatic lockout is canceled.
2 If you cannot remember your password and cannot return, press the [Shutdown] button to end
the FPIA-3000 application.
Account/Lockout settings
Lockout settings can be made for cases of failed logon.
1 Select the following items under [Account/Lockout settings] on the [Password/
account] screen.
Item FunctionsOFF Logon attempts will always be accepted, however many are
unsuccessful.
ON (with time restriction) Lockout the account, with a time limit.
ON (with no time restriction) Lockout the account, with no time limit.
XX times Logon error Set the allowable number of entry errors under [Logon ID], [Password]on the Logon screen. The setting range is 1–100. The number ofmistaken entries can be checked from [Reject Counts] in the [UserInformation Edit] window.
XX minutes Lockout Set the lockout duration (unit: minutes) for account locks (with timelimit). The setting range is 1–3,600 minutes.
Table 13-8: [Account/Lockout Settings] items
7/18/2019 FPIA3000 User Manual English August 2006
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006 13-11
Password Size
Set the number of characters which can be entered as an account password.
Up to 50 characters can be entered.
Limitation of Password reuse
When [Permit Password reuse.] is selected under [Limitation of password reuse] on the[Password/account] dialog screen, reuse of passwords can be permitted even after the
expiration of the password.
Also, if [Prohibit password reuse.] is selected and an expiration date is set for user registration
and user-edited passwords, the [Password Expiration Warning] dialog is displayed at login as
in Figure 13-10 from 14 days before the password expires. If you press the button that changes
password, the [Change password] dialog is displayed as in Figure 13-11.
At this time, use of the last password is not permitted. If you input the same password, a warning
like that in Figure 13-12 is displayed.
You can set which passwords cannot be reused by inputting a count next to [Prohibit password
reuse.]. Input “1” to prohibit reuse of the current password, “2” to prohibit reuse of the current
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005
Chapter 14 SYSTEM ADJUSTMENT
1. Adjusting the pressure of the pneumatic unit ............................................ 14-11.1 Adjusting the pressure ........................................................................ 14-1
2. Adjusting the pressure of the testing unit ..................................................14-22.1 Checking the pressure of the testing unit ............................................14-22.2 Pressure adjustment points................................................................. 14-32.3 Adjustment of rinse pressure .............................................................. 14-42.4 Adjusting the -0.05 MPa Pressure ...................................................... 14-5
7/18/2019 FPIA3000 User Manual English August 2006
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005 14-1
1. Adjusting the pressure of the pneumatic unit
Pressure and vacuum applied to the testing unit are supplied from the pneumatic unit. The methodfor adjusting compressed air pressure is as described below.
1.1 Adjusting the pressure
The method for adjusting compressed air pressure is as described below.
1 Turn on the testing unit.
The pneumatic unit switches on.
If the pneumatic unit has been turned OFF by the timer, press the [Pneumatic Unit] button on
[Testing/Analysis Parameters] dialog.
2 Check the test value of the 0.22 MPa pressure on the device status screen.The target range is 0.22±0.01 MPa. If the pressure is out of the target range, make sure that the
connection tube is not bent or disconnected, then check the pressure again.
If the pressure is still not within the target range, adjust as follows.
3 Turn the fastening screw counter-clockwise.
4 Use the 0.22 MPa Regulator to adjust the pressure to approximately 0.22±0.01 MPa.
Turn clockwise to increase and counter-clockwise to reduce the pressure.
Figure 14-1: Checking the pneumatic unit (compressed air pressure)
5 After making the adjustment, tighten the fastening screw.
NOTE: You cannot test samples and adjust pneumatic unit pressure at the same time. Beforeadjusting pneumatic unit pressure, check that no samples are being tested.
CAUTIONAlways adjust the pressure upward to the specified level. When the pressure istoo high, first lower it below the specified level, then raise it.
High
Low0.22 MPa Regulator
Fastening screw
7/18/2019 FPIA3000 User Manual English August 2006
14-2 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005
2. Adjusting the pressure of the testing unit
Pressure and vacuum applied to the testing unit are supplied from the pneumatic unit.
The pressure supplied to the testing unit is readjusted to the required pressure inside the testing unit.
The adjusted pressure in the testing unit is monitored by the instrument. Unless an error relating to
pressure is displayed, there is no need for readjustment.
2.1 Checking the pressure of the testing unit
1 Select [Test] – [Test].
The [Testing/Analysis Parameters] dialog appears.
Figure 14-2: [Testing/Analysis Parameters] dialog
2 Check the measured pressure values.
If the pressure is outside the normal range, adjust it as shown below.
NOTE: • You cannot test samples and adjust pneumatic unit pressure at the same time.Before adjusting pneumatic unit pressure, check that no samples are beingtested.
• If a pressure-related error is displayed, check the pressure of the pneumaticunit, then check the pressure of the testing unit.
7/18/2019 FPIA3000 User Manual English August 2006
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005 14-5
2.4 Adjusting the -0.05 MPa Pressure
Check the test value of the -0.05 MPa pressure on the device status screen. If the vacuum is outside
the target range, adjust as follows.
1 Loosen the fastening nut below the knob of the bellows pressure governor by turning it counter-
clockwise.2 While confirming the -0.05 MPa pressure value on the device status screen, adjust the vacuum
by turning the knob of the bellows pressure governor. Turning the knob clockwise raises the
vacuum pressure from -0.04 towards -0.05.
Target range: -0.053±0.005 MPa
Figure 14-4: -0.05 MPa pressure governor
3 After completing the adjustment, tighten the fastening screw.
CAUTIONAlways adjust the pressure upward to the specified level. When the pressure istoo high, first lower it below the specified level, then raise it.
Lock nut
7/18/2019 FPIA3000 User Manual English August 2006
3.1 Changing the fuse in the testing unit...................................................15-93.2 Changing the fuse in the pneumatic unit........................................... 15-103.3 Periodic Replacement Parts.............................................................. 15-11
4. Help ......................................................................................................... 15-114.1 Table of Contents.............................................................................. 15-114.2 Version information ........................................................................... 15-11
Revert from error Used when an error has occurred during a sequence.
Manual focusing Used for manual focusing.
Auto Focusing Used for automatic focusing. Refer to Chapter 3.
Background check Used for testing background values for the device.
Sheath liquid exchange Used to replace sheath liquid.
Compresson ON Used to cancel the pneumatic unit timer.
Cover open/close Used to manually open and close the cover.
Adjjustment of the flash brightness Used to adjust stroboscope brightness.
Stir motor adjustment Used to adjust the speed of the mixing motor.
Table 15-1: Names of sequences and their purposes
CAUTION• When measuring highly adhesive particles or samples with high particle
concentrations, check to make sure that there is no carryover. Ordinaryrinsing may not be sufficient. When there is carryover, perform theautomatic rinsing sequence.
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005 15-3
3 Dispense 5ml of a sample prepared to focus adjusting particle into the sample chamber, as
instructed by the action message. About focus adjusting particle, refer to Appendix F, FOCUS
ADJUSTING SAMPLE .
4 Press the [Start] button. Manual focus adjustment sequence starts, and after a while, particle
images will be displayed on the screen.
Check the images and click , , and buttons to adjust focus. Click on andto move the flow cell by the minimum distance in the direction of the arrow. Click on and
to move by five times the distance produced by clicking on and .
Figure 15-3: [Manual Focus Sequence] dialog
CAUTION• Since the captured particle images displayed in a real-time are observed
with eyes to make focus adjustment in the manual focus adjustment
sequence, an individual variation and an adjustment error will occur.• If a correct analysis result is required, perform the auto focus adjustment
sequence after the manual focus adjustment sequence has beencompleted.
15-6 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005
3 After connecting the sheath fluid, click the [Run] button in the dialog.
The sheath fluid primed in the testing unit will be started to exhaust.
When the exhausting the sheath fluid is completed, the following dialog will be displayed to
prompt an adjustment of the rinse pressure.
Figure 15-8: Adjustment of the rinse pressure dialog
4 Adjust the rinse pressure regulator so that the measured value becomes the target value. After
adjusting the rinse pressure, click the [Run] button in the dialog.The hydraulic system is rinsed by the connected sheath fluid, and the sheath fluid is then primed.
When priming the sheath fluid is completed, the sheath fluid box shown below will display the
sheath fluid name that is connected and primed.
CAUTION• It is recommended to replace the Teflon tube between the sheath inlet
nipple and the sheath container to avoid contamination of the sheath
fluid. In this case, cut the provided Teflon tube 4 mmID x 6 mmOD in anappropriate length to use.
• There is a potential to generate heat or poisonous gas when the
previous waste in the waste tank is chemically reacted with the newsheath fluid. It is recommended to replace by a new waste tank when thesheath fluid is replaced by a new type.
• Cap the disconnected sheath fluid container, and store in a safe place.
Discard the waste in the waste tank in accordance with the laboratory’s,local and government regulations.
CAUTION• There is a potential to generate heat or poisonous gas when the
previous waste in the waste tank is chemically reacted with the newsheath fluid. When a dialog shown in Figure 15-8 is displayed, replace
by a new waste tank.
7/18/2019 FPIA3000 User Manual English August 2006
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005 15-7
Figure 15-9: Displaying the sheath fluid name
5 After the sheath fluid priming is completed, the testing unit becomes ready.
CAUTION• Depending on the physical characteristics of the newly connected
sheath fluid, there may be a case that the new sheath fluid is mixed withresidual sheath fluid in the testing unit hydraulic system and emulsify(emulsion). When the sheath fluid turned emulsion, the analysis result is
drastically affected since the emulsion is detected as particles. When adifferent polar sheath fluid is to be replaced, use the [sheath fluid
replacing] sequence to replace one by an intermediate solvent toprevent sheath from turning into emulsion. For example, when the
PARTICLE SHEATH is desired to replace by toluene, it is effective touse an intermediate solvent of ethanol or 2-propanol to avoid emulsion.
PARTICLE SHEATH
Intermediate solvent (ethanol)
Intermediate solvent (ethanol)
1st step: PARTICLE SHEATH
2nd step: toluene
toluene
7/18/2019 FPIA3000 User Manual English August 2006
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005 15-11
3.3 Periodic Replacement Parts
The dialog below is displayed via [Maintenance] – [Parts Maintenance].
Figure 15-12: [Replacement Of Supply Parts] dialog
You can check the replacement date status for the parts replaced periodically: air filter, syringe
seal, and pinch valve tube.
The time to replace a part comes, an announcement dialog like the one in the figure below isdisplayed in the logon dialog during logon. These parts are replaced by staff from this company, so
please contact the staff responsible for service.
Figure 15-13: [Replacement Of Supply Parts] dialog
This dialog appears only for warning.
Pressing the [Close] button closes the announce dialog and the system operates normally.
4. Help
4.1 Table of Contents
Select [Help]-[Contents] to view PDF files of the FPIA-3000/3000S.
4.2 Version information
Select [Help]-[Version information] to show version information of the installed FPIA-3000.
7/18/2019 FPIA3000 User Manual English August 2006
5. List of display items ...................................................................................16-75.1 Particle diameter parameters .............................................................. 16-75.2 Form parameters................................................................................. 16-85.3 Other test data ....................................................................................16-95.4 Statistical analysis items ..................................................................... 16-95.5 Analysis data..................................................................................... 16-10
7/18/2019 FPIA3000 User Manual English August 2006
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005 16-1
1. Testing principles
The FPIA-3000/3000S tests particle images with the imaging flow cytometry and performs various
particle analysises.
Imaging flow cytometry means picking up flowing particles as a still image for the purpose of
image analysis. The following is an overview of how the system works.1) A sample supplied to the sample chamber is fed into a flat sheath flow cell by the sample
aspiration syringe.
2) The sample that has been fed into the flat sheath flow cell forms a highly laminar flow,
surrounded by sheath liquid.
3) A strobe light is flashed on the sample running through the flat sheath flow cell every 1/60
second. The duration of the stroboscope flash is very short, around 2µs, so that moving particles
can be captured as still images. The flow of the sample is very flat so that almost all the particles
in it can be seen in focus.
4) The particles are captured with a CCD camera and the captured images are processed in the
image-processing unit. In the image-processing unit, the edge of each particle observed is
extracted for calculation of the projected area and cut-out size of the particle.5) After testing is complete, the inside of the instrument is automatically rinsed and the sample,
together with the sheath liquid, is drained to the waste chamber.
Figure 16-1: Sample flow
1
2
4
5 3
Mixing motor
Mixing propeller
CCD camera
To the image-processing unit
Rinse chamber
Objectivelens
Waste chamber
Sample chamber
Flat sheath
flow cell
Sheath formation
syringe
Light source ofstroboscope
Sample aspirationsyringe
7/18/2019 FPIA3000 User Manual English August 2006
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005 16-5
4. Statistical analysis
This section explains the statistical analysis using the particle image parameters obtained by
analyzing the particle image.
(1) Calculation of the two-dimensional frequency dataThe number of particles within each divided range of the two-dimensional scattergram in which
the circular diameters are assigned to the X axis and the circularities are assigned to the Y axis
is calculated.
Values obtained by log-conversion of the circular diameters of the tested particles are used for
the X axis, and the 0.5-200 µm range is divided into 1,024 to calculate the frequency. For
circularity, the range of 0.2-1.0 is divided into 800 parts.
(2) Frame correction
Only those particles completely within the frame area captured are counted. The probability of
a particle being included within the frame area is inversely proportional to the size of the
particle. The larger the particle, the greater the possibility of being on the edge of the framearea. Frequencies are corrected taking these probabilities into account.
(3) Calculation of distribution frequency data
Values obtained by log-conversion of the circular diameters of the tested particles are used for
the X axis. The 0.5-200 µm range is divided into 1024 to calculate the frequency. In the case of
“number”, the number of particles (f0i) whose circular diameters d1 are within the range of di
to di + 1 is calculated. In the case of “volume”, the total volume (f3i) of particles whose circular
diameters are within the range of di to di + 1 is calculated.
(4) Calculation of form frequency data
With the circularity as the Y axis, the range from 0.2 to 1.0 is divided into 1024 to calculate the
frequency.
(5) Calculation of statistical analysis items
The statistical analysis items of 5.4 are calculated from the particle frequency data and the
particle form data.
(6) Calculation of the analysis data
The analysis data of 5.5 is calculated from the 2-dimensional frequency data.
7/18/2019 FPIA3000 User Manual English August 2006
16-6 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005
(7) Computation of 2-dimensional frequency data in Merge(LPF+HPF) mode.
In full range mode, superposition of 2-dimentional frequency data is calculated following
method.
Limit parameter and sample quantum are different between LPF mode and HPF mode.
Therefore, these frequency data cannot be simply added. Frequency conversion for differentsample quantum is calculated and frequency correction in the overlapped measuring range is
calculated.
Frequency conversion for different sample quantum
Since HPF quantum is different from LPF quantum, compensation of the frequency of HPF
measured data into frequency of LPF quantum is needed.
FH = ƒH x (VL / VH)
Frequency correction in the overlapped measuring range
When connecting up frequency data of HPF mode to LPF mode, particle size distribution
curve is not shown correctly because of the overlapping of the measured range. To solve this
problem, use this formula.
FLH = FH x {1-(δi-MIN) / (MAX-MIN)} + FL x {1-(MAX-δi) / (MAX-MIN)}
FLH : LPF/HPF frequency
FH : HPF frequency(after conversion)
FL : LPF frequency
f H : HPF frequency
VH : HPF quantum
VL : LPF quantum
MAX : upper limit of the overlapped measure range
MIN : lower limit of the overlapped measure range.
7/18/2019 FPIA3000 User Manual English August 2006
16-8 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006
5.2 Form parameters
Convex Hull area S envelope µm2 Measuredvalue
Area of the shape joining the projections onthe particle.
Analysis param-eters
Symbols Units Calculationmethods
Explanation
Circularity φ HL – 2×(S × π)1/2 / C The ratio between the circumferenceof a circle with the same areas as theprojected area and the peripherallength of the projected image.
Intensity Mean MI Measured value Average brightness of pixels in thearea of the particle.
Intensity SD ISD Measured value Standard deviation of brightness ofpixels in the area of the particle.
Feret Aspect ratio RD-Feret – Ratio of Feretdiameters(maximum = 1.0)
The aspect ratio of the horizontalrectangle tangent on the outside of theparticle.
Area/Box RS -Feret – S /(D H-Feret ×D V-Feret)
The ratio of the particle area to thearea of the tangent rectangle.Expresses the rectangularity of theparticle.
Aspect ratio(W/L)
RD-MajorAxis – B/L The ratio between Length and width.This means the spicular level.
Elongation EL –1-B/L
Expresses the slenerness of theparticle.
Aspect ratio(max dist)
R aspect – D v-max / D max The ratio of the largest perpendicularlength to the longest length.Expresses the spicularity of theparticle.
Solidity φ envelope – S / S envelope Level of irregularity relative to theenvelope.
Convexity R envelope – C envelope / C Level of irregularity relative to theenvelope.
HS circularity φ HS – 4x π xS/L2 The ratio of an area of the circle thatprojected area of the particle image
and peripheral length are same.
Major axis θL deg Measured value Direction where particle hasexpanded.
Table 16-2: Form parameters
Analysisparameters
Symbols Units Calculationmethods
Explanation
Table 16-1: Particle diameter parameters
7/18/2019 FPIA3000 User Manual English August 2006
16-10 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005
5.5 Analysis data
Upper% value Upper% – The parameter value at which thecumulative frequency in the range fromLdiscrete to Udiscrete reaches Upper%.
Lower% value Lower% – The parameter value at which thecumulative frequency in the range fromLdiscrete to Udiscrete reaches Lower%.
Analysisparameters
Symbols Calculation methods Explanation
Selected (#) Number Same as the number ofmedium particles
When particle limits are used within thescattergram and the numbers of large,medium and small particles are definedas below, the ratios are calculated usingthe formulae on the left.
Selected (%) % No. of limited particles/No. ofanalyzed particles ×100
Large (%) % No. of large particles/(No. oflarge particles + No. ofmedium particles + No. ofsmall particles) ×100
Middle (%) % No. of medium particles/(No.of large particles + No. ofmedium particles + No. ofsmall particles) ×100
Small (%) % No. of small particles/(No. oflarge particles + No. of
medium particles + No. ofsmall particles) ×100
Table 16-5: Analysis data
Analysisparameters
Symbols Calculation methods Explanation
Table 16-4: Statistical analysis items
Ldiscrete
Ldiscrete
Udiscrete
Udiscrete
Mediumparticles
Largeparticles
Smallparticles
7/18/2019 FPIA3000 User Manual English August 2006
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005
APPENDIX A INSTALLATION
1. Preparation for installation...........................................................................A-11.1 Accessories...........................................................................................A-1
1.2 Place and space for installation ............................................................A-51.3 Exterior dimensions...............................................................................A-7
2. Installation ...................................................................................................A-82.1 Dismantling the fastener for transportation ...........................................A-82.2 Mounting the Filter Assembly No. 24 (FPIA-3000S) ...........................A-152.3 Connecting the tubes ..........................................................................A-172.4 Connecting the power cables and connection cables .........................A-222.5 Grounding ...........................................................................................A-252.6 Installing the analysis PC ....................................................................A-26
3. Installation of Acrobat Reader ...................................................................A-30
7/18/2019 FPIA3000 User Manual English August 2006
WARNING • When a flammable organic solvent is used, install the instrument in afireless place. There is a potential to get fire or explosion when
evaporated gas catches fire.• When a flammable organic solvent is used, install the fire extinguisher
suitable for the organic solvent.• When the organic solvent is used, ventilate the instrument circumference
by installing a local ventilation device. There is a potential to get fire orexplosion when evaporated gas catches fire. There is also a potential toexpose to the toxic material.
7/18/2019 FPIA3000 User Manual English August 2006
A-6 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006
CAUTIONFor efficient use of the device, observe the following precautions.Temperature• Avoid extreme temperatures, whether low or high.
Use the instrument in an ambient temperature of 15 to 30°C.Humidity• Avoid places subject to water splashes, e.g., the vicinity of a sink, and
highly humid places.Use the instrument under a humidity of 30 to 85%.
Dust• Avoid dusty places, e.g., the vicinity of a blackboard, windows, etc.Vibration• Install the device on a vibration-free, flat and sturdy desk or table.Water Leakage• Do not put a water-filled container on the device.
Water leaking into the device can cause malfunction.Power supply• Rated voltage: AC100 V±10%
Frequency: 50/60±1 Hz
• Do not share the same outlet with noise-generating equipment such as acentrifugal separator, ultrasonic cleaner or thermostat. Also, keep a
distance of at least 2 m from such equipment.• The instrument must be installed with a ground connection. Refer to Appendix A, 2.5 Grounding for details of the grounding method.
Sunlight• Avoid locations that are subject to direct sunlight or are below
incandescent lights.Location• The FPIA-3000 has a fan at the rear. Ensure a space of at least 20 cm
between the rear of the device and the wall so as not to interfere withventilation. To facilitate maintenance and servicing, leave space of at
least 50cm on the sides and above the top surface.Data processing PC• Confirm that the resolution of the screen is 1280 × 1024 pixels with
[Display Properties].
• Confirm that all the settings within [Power Settings] are not set toautomatically turn OFF with the [Power Options Properties].
• Please uncheck the box of the item (Animate the windows when
minimized and maxmizing) whitch is in the [Advanced]-[Performance
Options] in the System Properties of Controlpanel.• Use the time and date set in the region and language option of Control
panel by default.
7/18/2019 FPIA3000 User Manual English August 2006
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005 A-15
2.2 Mounting the Filter Assembly No. 24 (FPIA-3000S)
In the FPIA-3000S, the Filter Assembly No. 24 is installed on the rear panel to filter the organic
solvent gas in the vacuum line to prevent any gas flowing into the pneumatic unit PU-17
compressor. Activated charcoal cartridge in the filter will adsorb gases.
1 Install the provided Filter Assembly No. 24 on the rear panel using the provided two screws
binding M5x8.
Figure A-16: Fixing the Filter Assembly No. 24
2 Connect the two tubes (1 each of Teflon tube and 1 each of Polyurethane tube) come from the
grommet to the fittings of the Filter Assembly No. 24.
3 Facing to the rear panel, connect the Teflon tube to the fitting located to the left of the filter andthe Polyurethane tube to the nipple located to the right side of the filter.
Figure A-17: Connecting tubes to the Filter Assembly No. 24
Filter Assembly No. 24
Screws binding M5x8
Teflon tubePolyurethane tube
7/18/2019 FPIA3000 User Manual English August 2006
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005 A-17
2.3 Connecting the tubes
Connect the pneumatic unit, the sheath fluid and drain tubes to the back of the testing unit.
1. Connecting to the pneumatic unit
1 Connect the vacuum output nipple (marked “VACUUM”) on the pneumatic unit to the vacuumsupply nipple (marked “VACUUM”) on the testing unit using the accessory polyurethane tube.
2 Connect the compressed air output nipple (marked “PRESSURE”) on the pneumatic unit to the
compressed air supply nipple (marked “PRESSURE”) on the testing unit using the accessory
polyurethane tube.
Figure A-19: Connecting the pneumatic unit
Compressed air output nipple (PRESSURE)
Vacuum output nipple (VACCUM)
Pneumatic unit
Testing unit
Polyurethane tubes
Compressed air supply
nipple
Vacuum supplynipple
7/18/2019 FPIA3000 User Manual English August 2006
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005 A-21
3. Connecting the tubes to the reagents and waste (FPIA-3000S)
Connect the sheath fluid, waste container, overflow waste fittings located on the rear panel using
the provided Teflon tube 4mmID x 6mmOD.
Follow the procedures below to connect to each fitting with the Teflon tube 4mmID x 6mmOD.
1 Cut vertically the point of the Teflon tube connecting in the fitting using a sharp knife.
2 Loosen the fixing nut of the fitting and insert the tubing prepared in the step (1) fully to hit to
the inner end.
3 Tighten the fixing nut of the fitting to secure the tube.
Figure A-23: Connecting the reagent and waste tubes
WARNING• When the organic solvent is used, open the sheath and the waste
containers, and connect them to the instrument right before testing. If
the sheath and the waste containers that contain solvent are left open, itmay cause fire or explosion after gas is filled in the room. There is also apotential to expose to the toxic material.
CAUTION• Install the sheath and the waste containers in a lower position than the
waste outlet nipple of the testing unit.• When the organic solvent is used, open the sheath and the waste
containers and connect them to the instrument right before testing. If thesheath and the waste containers are left open, evapolated gas will beignited and resulted in fire or explosion. There is also a potential toexpose to the toxic material.
A-28 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006
2. Installation of the FPIA-3000 application
Install the FPIA-3000 on the analysis PC.
1 Double click on [FPIA-3000(ENG).exe] on the CD.
The following dialog will be displayed.
Figure A-30: Welcome to the FPIA-3000 Setup Wizard dialog
2 Click on the [Next] button.
The following dialog will be displayed.
Figure A-31: Select installation folder
NOTE: Depending on the version of Windows, a dialog prompting you to update the system fileis displayed after double-clicking on [FPIA-3000(ENG).exe]. Click on the [OK] button.The system file is automatically updated and the computer restarts. Run the installationagain from step 1.
7/18/2019 FPIA3000 User Manual English August 2006
SPECIFICATION AND PERFORMANCE OF THE FPIA-3000/3000S
B-2 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006
Test Items and display items
1) Test Items
(Particle diameter items)CE Diameter
Max. Distance
Norm to max dist
Length
Width
Feret H Diameter (Horizontal)
Feret V Diameter (Vertical)
Martin H Diameter (Horizontal)
Martin V Diameter (Vertical)
Krumbein H Diameter (Horizontal)
Krumbein V Diameter (Vertical)PE Diameter
Area(pixels)
Perimeter
Area
Convex Hull perimeter
Convex Hull area
(Particle Shape items)
Circularity
Intensity Mean
Intensity SD
Feret Aspect ratioArea/Box
Aspect ratio (W/L)
Elongation
Aspect Ratio (max dist)
Convexity
Solidity
HS Circularity
Major axis
(Statistical analysis items)
Mean
ModeSD
CV
50%
Lower%
Upper%
NOTE: Only the surface area circular diameter and circularity analysis results when particlesheath liquid is used as the sheath liquid and dispersal agent are assured.
7/18/2019 FPIA3000 User Manual English August 2006
SPECIFICATION AND PERFORMANCE OF THE FPIA-3000/3000S
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005 B-3
(Analysis data)
Selected (#)
Selected (%)
Large (%)
Middle (%)
Small (%)
(Other items)
Detected (#)
Analyzed (#)
Density
Analyed volume
2) Display Items
Particle imageHistograms of measured itemsScattergrams of measured itemsCumulative distribution of measured itemsList of stored dataFrequency tables for measurement itemsGraph(Overlay-View)Graph(Trend-View) (analysis parameters, scattergrams)Meta dataSample Data DateSample NumberSample NameSample Group NameMeasurer name
7/18/2019 FPIA3000 User Manual English August 2006
2) CPU Intel Pentium4 3.0 GHz or faster / Hyper Threading Technology: ON
3) Memory 1.0 GB or greater
4) Hard disk drive 100 GB or greater5) Storage One 3.5-inch floppy disk drive
DVD ± R/RW (or DVD-RAM) drive ×1
6) Input/output ports At least four USB 2.0 ports
RS232C
Ethernet 10/100BASE-T ×1 port
7) Monitor 1280×1024 resolution or greater (at least 17-inch LCD monitor
recommended)
NOTE: • The diameters of latex particles 4016A, 4210A and 4240A used in this test areguaranteed by Duke Scientific Corporation on the basis of NIST (NationalInstitute of Standards and Technology) standards.
• Standard particles for FPIA by Sysmex are independently developed,produced and managed by Sysmex as standard substances for measurement
of average circularity and particle density by the FPIA-3000.
7/18/2019 FPIA3000 User Manual English August 2006
C-2 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006
Supplementary explanation
1) System ID number - Instrument Type:
The FPIA-3000 allows its user to set instrument type.
Input an ID up to 10 alphanumerics long.
Do not use "/" or "\" character.
2) System - Record operation - Automatic deletion“Use/Do not use”
The maximum number of samples stored into memory is 1000. You can set whether or not the
oldest sample data is automatically erased when there are more than 1000 samples for memory.
The default is set to “Do not use”.
3) System - Sample number - Sample number increment
“Use/Do not use”
You can set whether or not the sample number on the measurement and analysis conditions
screen is incremented automatically. The default is set to “Use”.
4) Sensor target temperature
This sets the target temperature for the sensor.
The default is a target temperature range of 25.0°C. The target temperature can be set in therange from 15.0°C to 30.0°C.
Since the target temperature is adjusted automatically when detection unit room temperature
correct is set to “Use”, the target temperature setting is only in effect when “Do not use” is set.
5) Detection unit room temperature correction
“Use/Do not use”
You can set whether or not to automatically adjust the temperature of the detection unit.
The default is set to “Use”.
6) System-Display-Toolbar
“[Valid / Invalid]”
Menu icons of the FPIA-3000 will be displayed if you select [Valid] or will not be displayed if
you select [Invalid].
7) System - Unit - Unit of density
Unit of concentration will be set /UL if you select "/UL" or /ML if "ML".
CAUTIONAttention must be paid to the following when detection unit room temperaturecorrection is set to “No”.(1) If the difference between the ambient temperature (room temperature) and the
target temperature is large, the detection unit temperature may not reach theset target temperature. Change the ambient temperature or the targettemperature.(Example: Ambient temperature 30.0°C, target temperature 15.0°C)
(2) Depending on the target temperature, it may take over an hour for thetemperature within the detection unit to stabilize.
Open the [Testing/Analysis Parameters] dialog screen and check the devicestatus.
(3) The measurement results are outside the assured range.
7/18/2019 FPIA3000 User Manual English August 2006
2. Serial communications ............................................................................... D-12.1 Hardware.............................................................................................. D-12.2 Communication Format........................................................................ D-12.3 Signal Level.......................................................................................... D-22.4 Protocols used in communication with the host ................................... D-22.5 Class A communications...................................................................... D-22.6 Class B communications...................................................................... D-32.7 Processing of communication errors with the host............................... D-42.8 Monitoring the receiver (host) side....................................................... D-4
3. Ethernet communications........................................................................... D-53.1 Hardware.............................................................................................. D-53.2 Software ............................................................................................... D-53.3 Text format ........................................................................................... D-63.4 Transmission format selection ............................................................. D-6
4. Data format................................................................................................. D-74.1 Test results (limited data)..................................................................... D-74.2 Histogram data..................................................................................... D-94.3 Scattergram data................................................................................ D-114.4 Extend data ........................................................................................ D-13
5. Particle form ............................................................................................. D-19
GAS SENSOR SETTING VALUES AND LIST OF FPIA-3000S RECOGNIZED ORGANIC SOLVENTS
E-2 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006
2. List of FPIA-3000S recognized organic solvents
Hydraulic parts used in the FPIA-3000S are made of materials that are resistant to the organic
solvents listed in the Table E-2 “List of Recognized Organic Solvents”.
CAUTION• Table E-2 “List of Recognized Organic Solvents” shows the applicability and
compatibility of the hydraulic parts against the organic solvent, well focusedparticle images may not be obtained depending on the viscosity and specificgravity of the organic solvent since a stable sheath is not formed. If the ignitionpoint, the boiling point, or the melting point is very low, there is a potential of afire or an explosion.
• FPIA-3000S specifications are assured only when the PARTICLE SHEATH isused as the sheath fluid. When an organic solvent is used, the particle imagemay be different due to the refractive index of the solvent used. Accordingly, theanalysis results of the particle diameter and the circularity may be different fromthose analyzed using the PARTICLE SHEATH.
No. Name of the Organic Solvent
1 2,2-dimethyl butane
2 2,3-dimethyl butane
3 2-n-butoxyl ethanol
4 4-hydroxy 4-methyl 2-pentanone
5 n-butyl acetate
6 n-hexane
7 n-heptane
8 α-terpineol
9 acrylic acid
10 ethyl acrylate
11 butyl acrylate
12 methyl acrylate
13 acrylonitrile
14 butoxy ethanol adipate
15 acetyl acetone
16 acetonitrile
17 acetophenone
18 acetone
19 methyl acetoacetate
20 aniline
21 amino alcohol
22 amyl chloronaphthalene
23 amyl naphthalene
Table E-2: List of Recognized Organic Solvents
7/18/2019 FPIA3000 User Manual English August 2006
F-2 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005
2. Preparation of focus adjustment sample
The focus adjustment particles are dispersed in the sheath fluid as a dispersion medium, and adjust
the concentration of approx. 30000 particles/ µL.
CAUTION• Auto focus adjustment may not be correctly functioned, if the sample is not
dispersed and the sample contains lots of agglutinated particles. Prepare thesample so that the particles are mono-dispersed.
• When the auto focus adjustment is performed using a sample that wasprepared in a long time ago, the automatic focusing may not be correctlyfunctioned due to re-agglutination of the particles. Use such a sample afterdispersing by using an ultrasonic dispersion device.
NOTE: • When melamine resign particles are dispersed in the organic solvent as a dispersionmedium, add an appropriate amount of the surfactant in accordance with the appliedorganic solvent to obtain a sample without agglutination.
• By using the ultrasonic dispersion device, a sample can be prepared with lesseragglutination.
7/18/2019 FPIA3000 User Manual English August 2006
3. Background Count Generated by Erosion.................................................. G-53.1 When using the Standard Magnification Unit ....................................... G-53.2 When using the High Magnification Unit .............................................. G-63.3 When using the Low Magnification Unit ............................................... G-7
4. Sample Temperature Arisen when Ultrasonic Scanned............................. G-7
7/18/2019 FPIA3000 User Manual English August 2006
G-4 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005
Figure G-5: Ultrasonic Scan Sequence
3 After setting each item, press the [Close] button to save the settings.
4 Run the program.
2.2 SOP Settings
Various setting items for the Ultrasonic dispersion unit can be registered in the SOP settings. When
analyzed with selecting the SOP settings that have registered with ultrasonic scan conditions,
ultrasonic scan will be performed automatically.
Refer to Chapter 8, SOP SETTINGS for more information on the SOP Settings.
CAUTION • When a sample is supplied to the sample mixing chamber, make sure that thetip of the horn is dipped in the sample. If ultrasonic scan were performedwithout dipping the tip of the horn in the sample, it may cause a permanentdamage to the electronic circuitry.
• When ultrasonic scan is performed, a background count arises due to theerosion.When analyzing with irradiating the ultrasonic dispersion unit, confirm that thebackground count by the erosion will not affect the results significantly. Refer tothe "4.Sample Temperature Arisen when Ultrasonic Scanned" for thebackground count by the erosion.The erosion is defined that the tip of the horn is damaged by the ultrasonicshock generated by itself and a part of the horn is disengaged.
• It should be noted that when ultrasonic scan is performed for a prolonged periodof time, the sample temperature will be arisen due to the heated horn. Refer to
the Appendix G:"4.Sample Temperature Arisen when Ultrasonic Scanned" forthe arisen temperature.• The sample dispersion effect will depend on the ultrasonic scan strength,
sample volume and the type of dispersion medium.
WARNINGThe Ultrasonic Dispersion Unit shall be used only if its sheath liquid is "PARTICLESHEATH" or "Water".Do not use any combustible liquid(such as alcohol) as sheath liquid because ofignition.
Testing sequence
Preparing
in prior totesting
Displaying a
dialog waitingfor sample
introduction
Ultrasonic
irradiation inprior to testing
Testing
Sample
introduction
Pre-Test Irradiation Testing
Ultrasonic scan
is performed in
prior to testing
for the time set
in the "Pre-Test
Irradiation Time".
Ultrasonic scan
is performed if
"Mid-Test Irradiation"
is set to "Apply".
7/18/2019 FPIA3000 User Manual English August 2006
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006
APPENDIX H HIGH/LOW MAGNIFICATION LENS UNIT
MANUAL (OPTION)
1. FPIA-3000 Optional Lens Unit.................................................................... H-11.1 Features ............................................................................................... H-11.2 General Information of the Lens Unit ................................................... H-2
3. Preparation for testing .............................................................................. H-113.1 Starting the analysis program ............................................................ H-11
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006 H-11
3. Preparation for testing
3.1 Starting the analysis program
3.1.1. Starting the testing unitTurn ON the power switch by referring to Chapter 3: "2. Turning on the device".
3.1.2. Starting the Analysis Program
1 Double click on the FPIA-3000 shortcut on the Windows desktop.
The logon dialog box appears.
2 Enter the logon ID and password as in table H-6. Press the [Setting] button to change the
objective lens.
Figure H-14: [Logon] dialog
NOTE: • If the FPIA-3000 does not start, use one of the procedures below to start it.- Click through [Windows taskbar ] - [Start] button - [Programs] - [Sysmex] - [FPIA-3000].- Double click on [FPIA-3000.exe], which is directly below the install folder.
Set Items Explanations
Logon ID Enter the previously assigned login ID.Refer to the FPIA-3000 Operator’s Manual, Chapter 13, USER
AUTHENTICATION for the acceptable characters.
Password Enter the previously assigned password.The input password will be displayed as a string of asterisks "*". Refer to theFPIA-3000 Operator’s Manual, Chapter 13, USER AUTHENTICATION for the
acceptable characters.
[Start Testing Unit?]check box
Place a check mark in the [Start Testing Unit] check box, when only the storeddata is reviewed and you do not need to star t the Testing Unit.
[Setting] button Displays [Logon Setting] dialog.
[OK] button Starts up the Testing Unit and analysis software.
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006 H-15
If the following dialog was displayed during the startup of the Testing Unit, press the [Alarm Reset]
button, stop the alarm, and press the [Error Recovery] button after the button becomes effective.
Figure H-21: [Error History] dialog
When the error is resolved, the start process will continue.
If the error cannot be resolved, refer to Chapter 7, TROUBLESHOOTING.
Execute the process described in the 3.1.4."Process executed after replacing the Lens Unit".
If the FPIA-3000 is run without replacing the Lens Unit, execute the procedures described in the
3.1.5."Background Check" and following sections.
NOTE: • After switching the instrument power on, wait approximately one hour for its internaltemperature to stabilize.
• Once the internal temperature of the instrument has stabilized, follow the procedureon the next page to run a background check and auto focus adjustment.
The selected lens magnification is displayed to the right of the measurement and analytical
conditions.
NOTE: • To perform the automatic focus adjustment, prepare 5 mL or more of the certainconcentration of the adjustment fluid to make the focus adjustment latex dispersed inthe PARTICLE SHEATH.
Lens Unit Original Latex fluid Concentration
High Magnification Unit DUKE Latex fluid 5100A(1.0 µm)
Approx. 200,000/ µL
Low Magnification Unit DUKE Latex fluid 5312A(3.1 µm)
Approx. 5,000/ µL
Standard Unit DUKE Latex fluid 5200A(2.0 µm)
Approx. 30,000/ µL
Table H-7: Latex fluid for the automatic focus adjustment
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006 H-19
5. Analyze
For the data analysis, refer to Chapter 5, RECORD DISPLAY AND PROCESSING.
Followings are the notes specifically applied to the optional Lens Unit.
CAUTIONTwo records which were obtained in the different magnifications will not bemerged.Two records which were obtained in the different magnifications will not beoverlapped of their graphs.Two records which were obtained in the different magnifications will not begraphed or trended.
H-22 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006
Test items and display items
1) Analysis parametersThe analysis parameters are the same as the standard unit. Refer to Appendix B,
SPECIFICATION AND PERFORMANCE OF THE FPIA-3000/3000S .
2) Displayed Items
The display items are the same as the standard unit. Refer to Appendix B, SPECIFICATION
AND PERFORMANCE OF THE FPIA-3000/3000S .
Required reagents and their consumption
At startup
During Testing
At shutdown
When replacing sheath fluid
Operating Environment
Ambient temperature: 15 - 30ºC
Relative humidity: 30 - 85%
Installation conditions: Avoid direct sunlight, dust, vibration, acidity and condensation.
NOTE: When the sheath fluid and dispersion media are not PARTICLE SHEATH, analysisresults other than the area, circular diameter and circularity are not guaranteed.
Reagents used Consumption
PARTICLE SHEATH (for automatic rinse) Approx. 90 mL
PARTICLE SHEATH (for background checking) Approx. 70 mL
PARTICLE SHEATH (for automatic focus adjustment) Approx. 170 mL
NOTE: • The diameters of latex particles 3800A, 4205A and 4220A used in this test areguaranteed by Duke Scientific Corporation on the basis of NIST (National Institute ofStandards and Technology) standards.
• Standard particles for FPIA by Sysmex are independently developed, produced andmanaged by Sysmex as standard substances for measurement of average circularityand particle density by the FPIA-3000.
7/18/2019 FPIA3000 User Manual English August 2006
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006 H-27
Test items and display items
1) Analysis parametersThe analysis parameters are the same as the standard unit. Refer to Appendix B,
SPECIFICATION AND PERFORMANCE OF THE FPIA-3000/3000S .
2) Displayed Items
The displayed items are the same as the standard unit. Refer to Appendix B, SPECIFICATION
AND PERFORMANCE OF THE FPIA-3000/3000S .
Required reagents and their consumption
At startup
During Testing
At shutdown
When replacing sheath fluid
Operating Environment
Ambient temperature: 15 - 30ºCRelative humidity: 30% to 85%
Installation conditions: Avoid direct sunlight, dust, vibration, acidity and condensation.
NOTE: When the sheath fluid and dispersion media are not PARTICLE SHEATH, analysisresults other than the area, circular diameter and circularity are not guaranteed.
Reagents used Consumption
PARTICLE SHEATH (for automatic rinse) Approx. 90 mL
PARTICLE SHEATH (for background checking) Approx. 70 mL
PARTICLE SHEATH (for automatic focus adjustment) Approx. 170 mL
NOTE: • The diameters of latex particles 4203A, 4220A and 4280A used in this test areguaranteed by Duke Scientific Corporation on the basis of NIST (National Institute ofStandards and Technology) standards.
• Standard particles for FPIA by Sysmex are independently developed, produced andmanaged by Sysmex as standard substances for measurement of average circularityand particle density by the FPIA-3000.
7/18/2019 FPIA3000 User Manual English August 2006
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005
APPENDIX I PART11 SOFTWARE MANUAL (OPTIONAL)
1. FPIA-3000 Optional Unit (Software Conforming to Part 11)......................... I-11.1 Features ................................................................................................. I-1
2. Installation of the Part 11 Software .............................................................. I-22.1 Install...................................................................................................... I-2
3. Using the FPIA-3000 Part 11 Software ........................................................ I-33.1 Preparation for testing............................................................................ I-33.2 Testing ................................................................................................... I-33.3 Stored Data Display and Processing ..................................................... I-33.4 Error Treatment...................................................................................... I-33.5 SOP settings .......................................................................................... I-33.6 User Verification..................................................................................... I-33.7 Audit Trail Function ................................................................................ I-4
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005 I-1
1. FPIA-3000 Optional Unit (Software Conforming to Part 11)
1.1 Features
The optional software conforming to 21 CFR Part 11 is based on the guidelines shown by the
United States Food and Drug Administration (FDA), and provides following functions.
• Audit trail function
FPIA-3000 operation histories are stored in a record as an audit trail.
• Conforms to the electronic signature
Analysis results can be output in the form of a PDF file with an electronic signature, which is
a feature of PDFs.
• Individuals authentication function
Different access levels can be set for different logon users so as to limit the features that users
can use according to their access level. FPIA-3000 standard functions include this. For more
information refer to Chapter 13, USER AUTHENTICATION .
NOTE: • The 21 CFR (Code of federal regulations) Part 11 is a mandatory regulation of theUnited States Food and Drug Administration for the electronic submission, electronicrecords and electronic signature of the application documents.
• The PDF (Portable Document Format) is a publicly available file format used todistribute and exchange the electronic document through the internet.
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005 I-3
3. Using the FPIA-3000 Part 11 Software
3.1 Preparation for testing
Prepare the sample by referring to the Chapter 3, PREPARATIONS FOR TESTING.
3.2 Testing
Test the sample by referring to the Chapter 4, TESTING.
3.3 Stored Data Display and Processing
Table I-1 shows the differences between the standard application and the Part 11 application.
3.4 Error Treatment
For the procedures to treat an error, refer to Chapter 7, TROUBLESHOOTING.
3.5 SOP settings
The differences from the FPIA-3000 standard application are shown below.
The [Del.SOP] and [Save] button are removed in the SOP Settings dialog, and deleting and
saving the SOP are not possible. For details, refer to Chapter 8, SOP SETTINGS .
3.6 User Verification
The differences from the FPIA-3000 standard application are shown below.
The [Delete] button is removed in the user information dialog, and deleting the registered user is
not possible.
CAUTION• If the number of stored records exceeds 1000, a message “You cannot run.
The number of records exceeds the upper limit.” is displayed and you cannotrun a test.If this happens, select [File] - [New] to create a new database to store testrecords. For details, see Chapter 5 RECORD DISPLAY AND PROCESSING10.2 NEW in this manual.
• You can run the background check and automatic focus adjustment, even if thenumber of stored records exceeds 1000.
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised December 2005 I-9
4.5 Filter
Click on the [Filter ] button in the audit trail dialog to display the [Audit Trail Filter ] dialog, as
shown in Figure I-3.
Place a checkmark in the [Specified Execute as for the filter ], check the audit trail filter items
listed in the Table I-4, and click the [OK] button to display the filtered audit trail list in the audit
trail list dialog.
Figure I-3: [Audit Trail filter ] dialog
Item Explanation
Check box to Specify the filter Place a checkmark to validate the filter conditions in the audittrail. Filtered audit trails are listed.
Check box for user name and its listbox
Place a checkmark in the user name check box, and username can be selected in the user name list box, which is usedto filter the audit trails list.
Check box for event and its list box Place a checkmark in the event check box, and event can beselected in the event list box, which is used to filter the audittrails list.
Check box for date, today/date radiobutton, and date list box
Place a checkmark in the date check box, select a radiobutton either today or specified date, and the specified datecondition is used to filter the audit trails list. When specifieddate radio button is selected, date range can be specified.
Table I-4: Audit trails filter items
7/18/2019 FPIA3000 User Manual English August 2006
5. Starting the analysis program.................................................................... J-105.1 Starting the analysis program ............................................................. J-105.2 Replacing the Sheath Fluid................................................................. J-125.3 Process executed after replacing the FPIA-3000 Dark Field Unit....... J-15
5.4 Checking Light Field System............................................................... J-15
9.1 Quality Control .................................................................................... J-289.2 Adjustment of the flash brightness...................................................... J-28
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006 J-1
1. Overview
1.1 Overview
FPIA-3000 Dark Field Unit is an optional unit for particle imaging and image analysis using dark
field optical system. The Dark Field Unit can be used for measurement after replacement andadjustment of the illumination lens, and software setting change. The unit can be replaced by each
customer.
* The unit is not supporting the High and Low Magnification options.
1.2 Principle of Dark Field Test
The ring slit for dark field illumination is used to shut out the direct illumination to the objective
lens.
The background is dark since the direct illumination is shut out. When particles exist, scattered light
by particles reaches to the objective lens, and the bright white image of the particle can be obtained.
(Refer to the figure below.)
In the case of dark filed mode, only the images brighter than the background are recognized as
particles, and analyzed.
Figure J-1: Principle of Dark Field Test
Ring slit
Sample
Condenser lens
Dotted line arrow : Scattered light (light scattered by particles reaches to the objective lens)
Solid line arrow : Illumination
Objective lens
7/18/2019 FPIA3000 User Manual English August 2006
J-10 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006
5. Starting the analysis program
5.1 Starting the analysis program
Start the analysis program as follows.
1 Turn ON the power switch of the testing unit by referring to the Chapter 3, Turning on the device.
2 Double click on the FPIA-3000 shortcut on the Windows desktop.
The logon dialog box appears.
3 Enter the logon ID and password as in table J-6. Press the [Setting] button to change the Lightfield system.
Figure J-14: [Logon] dialog
NOTE: • If the FPIA-3000 does not start, use one of the procedures below to start it.- Click through [Windows taskbar ] - [Start] button - [Programs] - [Sysmex] - [FPIA-3000].- Double click on [FPIA-3000.exe], which is directly below the install folder.
Set Items Explanations
Logon ID Enter the previously assigned logon ID.Refer to the FPIA-3000 Operator's Manual, Chapter 13, USER
AUTHENTICATION for the acceptable characters.
Password Enter the previously assigned password.The input password will be displayed as a string of asterisks “ * ”. Refer to theFPIA-3000 Operator's Manual, Chapter 13, USER AUTHENTICATION for theacceptable characters.
[Start Testing Unit?]check box
Place a check mark in the [Start Testing Unit] check box, when only the storeddata is reviewed and you do not need to start the Testing Unit.
[Setting] button Displays [Logon Setting] dialog.
[OK] button Starts up the Testing Unit and analysis software.
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006 J-13
2 Connect the sheath fluid to be used to the sheath fluid aspiration nipple.
Figure J-19: Sheath fluid replacement
3 Check that the tube is correctly connected to the sheath fluid aspiration nipple, then press the
[Run] button.The following dialog will be displayed.
Figure J-20: [Cleaning pressure settings] dialog
4 Set the cleaning pressure to match the sheath fluid used.
When the pressure display turns green, press the [Run] button. (See Chapter 14, 2.3.
"Adjustment of rinse pressure") The startup process runs.
NOTE: • After switching the instrument power on, wait approximately one hour for its internaltemperature to stabilize.• Once the internal temperature of the instrument has stabilized, follow the procedure
on the following pages to run a background check and auto focus adjustment.
J-14 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006
If the following dialog was displayed during the startup of the Testing Unit, press the [Stop
Alarm] button, stop the alarm, and press the [Recover ] button after the button becomes effective.
Figure J-21: [Error History] dialog
When the error is resolved, the start process will continue.
If the error cannot be resolved, refer to Chapter 7, TROUBLESHOOTING.
Execute the process described in the 5.3 “Process executed after replacing the FPIA-3000 DarkField Unit” after switching to the FPIA-3000 Dark Field Unit.
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006 J-15
5.3 Process executed after replacing the FPIA-3000 Dark Field Unit
Immediately after replacing to the FPIA-3000 Dark Field Unit, it is necessary to execute the
sequence of the manual focus adjustment.
When the background check dialog is displayed, press the [No] button to skip the background
check.
Figure J-22: [Background Check] dialog
When the automatic focusing adjustment dialog is displayed, press the [No] button to skip the
automatic focusing adjustment.
Figure J-23: [Focus Adjusting] dialog
Perform the manual focus adjustment, by referring to Chapter 15: "2.3 Manual focusing" .
Then perform the background check and the automatic focus adjustment by referring to Chapter 3:"3.3 Background Check" and Chapter 3: "3.4 Auto Focusing" .
5.4 Checking Light Field System
In the case of Dark Field Unit, “DF” appears in the title of the main screen.
Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006 J-25
8. Extended Analysis 1-5
These items are valid for extending analyses that can be used on the [Analysis Results] screen.
This section explains extending analysis 1 as typical.
1) Analysis Type
“Rectangular analysis/Polygonal analysis”
You can select either rectangular analysis, which limits through normal discriminationoperations, or polygonal analysis, which limits by specifying points on the scattergram.
2) Size Upper Limit
You can set the upper value for the X-axis direction particle diameter.
This is only valid when the analysis type is rectangular.
3) Size Lower Limit
You can set the lower value for the X-axis direction particle diameter.
This is only valid when the analysis type is rectangular.
4) Shape Upper Limit
You can set the upper value for the Y-axis form.
This is only valid when the analysis type is rectangular.5) Shape Lower Limit
You can set the lower value for the Y-axis form.
This is only valid when the analysis type is rectangular.
6) Polygon Limit Point
These show the polygonal limit points on the scattergram.
This is only valid when the analysis type is polygonal.
The limit points can only be changed on the [Analysis Results] screen.
7/18/2019 FPIA3000 User Manual English August 2006
J-28 Sysmex FPIA-3000/FPIA-3000S Operator's Manual -- Revised August 2006
9. Maintenance
9.1 Quality Control
Maintain condition of the instrument by performing the quality control since the measurement
results are influenced by flash brightness in the dark field mode. If the condition is out of the qualitycontrol range, "9.2 Adjust ment of the flash brightness" should be performed.
For detail of the quality control, refer to Chapter 6 . In the case of Dark Field Unit, display the
quality control dialog by selecting [Maintenance] - [Quality Control]. The Light field system
combo box in the right side of the dialog becomes valid for selecting dark field or bright field.
9.2 Adjustment of the flash brightness
Use PARTICLE SHEATH as sheath fluid.
Use the polystyrene latex particle of diameter 2µm as a sample. (Refer to the following Flash
Brightness Adjustment Sample)
Be sure to perform the automatic focusing adjustment prior to the adjustment of the flash
brightness.
Adjust flash brightness to an optimized value automatically. Perform the following procedures
when the FPIA-3000 Dark Field Unit is being used.
1) Select and perform [Maintenance] - [Sequence] - [Adjustment of flash brightness(DF)].
After processing of the previous sequence is completed, the following dialog appears.
Figure J-26: [Adjustment of flash brightness Sequence] dialog
Dispense 5ml of the prepared flash brightness adjustment sample into the testing unit sample
chamber.
2) Press the [Start] button. Adjustment of flash brightness sequence proceeds.
Change logon user ............................... 13-15Change password ................................... 13-1Changing hardware ...................................J-4Changing the display of the Analysis Results
screen .................................................. 5-34Changing the fuse in the pneumatic unit
............................................................ 15-10Changing the fuse in the testing unit ...... 15-9Changing the record display ................... 5-17Check instrument status ........................... 4-2Checking Light Field System ...................J-15Checking the pressure of the testing unit
.............................................................. 14-2Compressor ON ...................................... 15-8Connecting the power cables and connection
cables .................................................. A-22Connecting the tubes ..............................A-17Cover open/close .................................... 15-8
Data format ...............................................D-7Extend data ........................................D-13Histogram data .....................................D-9Scattergram data ...............................D-11Test results (limited data) .....................D-7
Deleting a quality control data .................. 6-7
Deleting a quality control file ..................... 6-5Deletion ................ ................................... 5-16Detail Results ................... ....................... 5-43Detailed information display .................... 5-37Dismantling the fastener for transportation
Display of analysis results ...................... 4-14Display of error history .............................. 7-3Display reset ........................................... 5-13Disposal ............................................. ..... 2-17
E
Electromagnetic Compatibility (EMC) ....... 1-7Emergency stop ........................................ 2-6Error code list ............................................ 7-6Error message (in alphabetical order) ...... 7-4Error message list by function .................. 7-7Error messages and troubleshooting ........ 7-9Ethernet communications .........................D-5
Hardware ..............................................D-5Software ...............................................D-5Text format ...........................................D-6Transmission format selection .............D-6
Explanation of labeling .............................. 1-1Exterior dimensions ..................................A-7
F
File menu items ...................................... 5-45File Settings .............................................. 6-2
Import ...................................................... 5-48Installation location ................................... 2-7Installation of Acrobat Reader ................A-30Installing and moving the instrument ........ 2-7Installing the analysis PC ........................A-26Instrument inspection ................................ 3-1
Adjustment of the flash brightness ..... J-28Flash Brightness Adjustment Sample
.......................................................... J-29Quality Control .................................... J-28
Manual focusing ...................................... 15-2Measurement condition settings ............... 4-6Merging records ...................................... 5-16Meta Data ............................................... 5-42Mounting the Filter Assembly No. 24 (FPIA-
3000S) ..................................................A-15Moving records ....................................... 5-20Multi Scattergrams .................................. 5-39
N
Names and functions of the components of theFPIA-3000/3000S ................................. 2-11Front panel/right front panel ............... 2-11Pneumatic unit .................................... 2-15Rear side of the testing unit ................ 2-13
Names of components of the testing unit .. 2-3New ......................................................... 5-46
O
Open ....................................................... 5-47Other test data .................... .................... 16-9Overview of quality control ........................ 6-1Overview of the device .............................. 2-1
P
Packaging ................................................. 2-7Particle analysis ...................................... 16-3Particle diameter parameters .................. 16-7Particle form ............................................D-19Particle image list .................................... 5-35PDF Print ................................................ 5-53Periodic Replacement Parts ................. 15-11Place and space for installation ................A-5Preparation of focus adjustment sample ...F-2Preparing a sample ................................. 4-11Pressure adjustment points .................... 14-3Principle of Dark Field Test ....................... J-1Print format ............................................. 12-1
Deletion ............................................12-24Importing File ......................................12-2New format creation and editing ......... 12-5Preview ............................................. 12-25Saving .............................................. 12-24Saving Files - All Data ........................12-4
Saving Files - Selected Data .............. 12-4Printer paper ............................................. 3-1Printing .................................................... 5-52Process executed after replacing the FPIA-
3000 Dark Field Unit ............................. J-15Processing analysis results .....................5-18Property ..................................................12-8
Character (Label) ...............................12-9Cumulative frequency .......................12-22Frequency Table .............................. 12-21Graph (Overlay-View) .......................12-12Graph (Overlay-View) Table ............. 12-15
Quality control ...........................................6-1
R
Reagents ............................................2-8, 3-1Reanalyze ......................................5-15, 5-22Record Display and Processing ................ 5-1Replacing the Sheath Fluid ..................... J-12Resetting the alarm ...................................7-1Revert from error .....................................15-2Reverting from an error .............................7-2
S
Sample information revision .................... 5-14Sample information setting .......................4-4Sample Temperature Arisen when Ultrasonic
Scanned ................................................ G-7Save ........................................................ 5-50Screen display of the data processing unit
Class A communications ......................D-2Class B communications ......................D-3Communication Format ........................D-1Hardware .............................................D-1
Monitoring the receiver (host) side .......D-4Processing of communication errors with
the host .................... .........................D-4Protocols used in communication with the