SPECIAL FEATURE SPECIAL FEATURE March 2014 • gasworld | 37 www.gasworld.com/specialfeatures 36 | gasworld • March 2014 Campylobacter jejuni is today regarded as the biggest cause of food- borne infection in the western world. For example, scientists believe that contaminated bottled water could account for 12% of infections by this bacterium. Listeria monocytogenes (Lm) is regarded as emerging because the role of food in its transmission has only recently been recognised. Mycotoxins are toxic chemical products formed by mould species including Penicillium, Aspergillus and Fusarium. These moulds can readily colonise food crops such as cereal grains, nuts and fruits, either in the field or post harvest. Consumption of foods produced from these commodities poses a potential risk to human health because the toxins are resistant to various food processes, including heat treatment. Microbiology laboratories doing classical testing use specialty gas mixtures containing carbon dioxide, hydrogen, nitrogen or oxygen to create aerobic and anaerobic controlled atmospheres in controlled temperature incubators to stimulate and control This contamination is a public health concern and has periodically caused outcry among environmental and health advocates. The benzene results from decarboxylation of the preservative benzoic acid in the presence of ascorbic acid (vitamin C), especially under heat and light. Contemporary threats and detection As little as 20 years ago, three of the four most significant foodborne pathogens – Campylobacter, Listeria and enterohaemorrhagic E. coli (EHEC) – were unrecognised as causes of foodborne illness. I n recent years, Chinese officials have come under intense public and international pressure to intensify efforts to ensure safer food, after melamine-tainted milk products from the 2008 scandal in which six babies died and 300,000 babies fell ill, re-emerged in several stores around the country. This tragedy brought the issue of food safety under the world spotlight. The World Health Organisation (WHO) has stated that foodborne diseases and threats to food safety constitute a growing public health problem. Unsafe food is the cause of many acute and lifelong diseases, ranging from diarrhoeal diseases to various forms of cancer. WHO estimates that foodborne and waterborne diarrhoeal diseases taken together kill about 2.2 million people annually, 1.9 million of them children. Many world governments, particularly those in developed countries, have established dedicated official bodies to develop, monitor and enforce food safety directives and regulations. The EU parliament is informed on food safety matters by the European Food Safety Authority (EFSA). In the US, the Food and Drug Administration (FDA) publishes the Food Code, a model set of guidelines and procedures that assists food control jurisdictions by providing a scientifically sound technical and legal basis for regulating the retail and food service industries, including restaurants, grocery stores and institutional foodservice providers such as nursing homes. However, in the wake of the dioxin poisoning scare in Europe in 2011 which sparked import bans on some German farm products, we are reminded that the threat to food safety very much exists even within mature economies and that analysis of food and beverage produce is critical. Gas chromatography- mass spectrometry (GC-MS) is fast emerging as the analytical tool of choice for such forensic food analysis (see An Introduction to…GC-MS in the food sector, page 62). “Amid heightened concerns about food safety in many parts of the world, GC-MS comes to the fore as a very important analytical technique,” says Stephen Harrison, Head of Specialty Gases and Specialty Equipment at Linde Gases. “It is one of few techniques to determine exactly what is in a food “Amid heightened concerns about food safety in many parts of the world, GC-MS comes to the fore as a very important analytical technique...” sample. Characterised by its quick screening abilities, GC-MS has been widely heralded as the ‘gold standard’ for forensic substance identification.” This sophisticated scientific technique, which is routinely used by the FDA in the US to detect dioxin, may well have been successfully employed to trace the source to a single fats manufacturer in the German egg scare of 2012. EU rules allow no more than a one part per trillion of dioxin in food for human consumption. Over a period of two weeks, batches of eggs and a shipment of pork with 1.5 trillionths were found. The German feed- mixing company supplied 110 egg farms, 651 piggeries and other farms in four German states, federal officials stated. Dioxin and chemically-related compounds (referred to collectively as dioxin-like compounds or DLCs) are a group of environmental contaminants found throughout the world. While German officials have said that eaten occasionally, the excess is unlikely to make anyone sick, the EFSA suggests that exposure to DLCs may lead to a variety of adverse health effects including reproductive and developmental problems, cardiovascular disease, increased diabetes and increased cancer. As DLCs tend to accumulate in the fat of food-producing animals, consumption of animal-derived foods, such as meat, poultry, eggs, fish, and dairy products, is considered to be the major route of human exposure to low levels of DLCs. “Applying a qualitative technique in a detective-like fashion in this crisis situation was critical. Samples could be checked right back through the food chain until analysts reached a point where the chemical did not show up in the sample – indicating that this is where it entered,” says Harrison. Similar scares have occurred in the beverage industry, one of the most notable being the benzene in soft drinks episode of the 1990s. Benzene levels are regulated in drinking water nationally and internationally, and in bottled water in the US, but only informally in soft drinks. Benzene in soft drinks is of some concern due to its carcinogenic nature. Food processing Part 1: Ensuring food safety and integrity