Field portable GC/MS in food safety

Dr. Joeri VercammenManaging Expert

www.is-x.com

Applications forField-Portable GC/MS

in Food Safety• Mass Range: 45 to 500 Daltons Mass Range: 45 to 500 Daltons

“Portable instruments will enable more lab work to be moved to the sampling site, providing real-time results and greatly improving response time and assay value.

Robert Stevenson, Am. Lab., Jan. 2012

Guide sampling (5%)

Guarantee sampleintegrity (5%)

Release of incominggoods (50%)

Healt & safety (30%)

Process control (10%)

Enquiry @ 375 labs

Applications?

• Boar taint markers• Shelflife & freshness• Flavour and fragrance• Toxins• Authenticity• Packaging• …

1

Strawberries

2.00 min

Ethyl acetate (87%)

Butanoic acid, ethyl ester (86%)

Hexanoic acid, ethyl ester (77%)

0.0E+00

5.0E+09

1.0E+10

1.5E+10

2.0E+10

2.5E+10

0 2 4 6 8 10 12 14

Time (Days)

Butanoic acid, ethyl ester Butanoic acid, 2-methyl-, ethyl ester 1-Butanol, 3-methyl-, acetate

Hexanoic acid, ethyl ester Acetic acid, hexyl ester Benzoic acid, ethyl ester

Ethyl acetate Propanoic acid, 2-methyl-, ethyl ester

0.0E+00

5.0E+09

1.0E+10

1.5E+10

2.0E+10

2.5E+10

0 2 4 6 8 10 12 14

Time (Days)

Butanoic acid, ethyl ester Butanoic acid, 2-methyl-, ethyl ester 1-Butanol, 3-methyl-, acetate

Hexanoic acid, ethyl ester Acetic acid, hexyl ester Benzoic acid, ethyl ester

Ethyl acetate Propanoic acid, 2-methyl-, ethyl ester

2

Chicken Filet

0.0E+00

5.0E+08

1.0E+09

1.5E+09

2.0E+09

2.5E+09

3.0E+09

3.5E+09

4.0E+09

4.5E+09

0 1 2 3 4 5 6 7 8 9

Time (Days)

>Disulfide, dimethyl >Dimethyl trisulfide

>Ethanol, 2-(2-ethoxyethoxy)- >Benzene, 2,4-diisocyanato-1-methyl-

>2H-Benzimidazol-2-one, 1,3-dihydro-5-methyl- >Pyridine, 4-(1-pyrrolidinyl)-

0.0E+00

5.0E+08

1.0E+09

1.5E+09

2.0E+09

2.5E+09

3.0E+09

3.5E+09

4.0E+09

4.5E+09

0 1 2 3 4 5 6 7 8 9

Time (Days)

>Disulfide, dimethyl >Dimethyl trisulfide

>Ethanol, 2-(2-ethoxyethoxy)- >Benzene, 2,4-diisocyanato-1-methyl-

>2H-Benzimidazol-2-one, 1,3-dihydro-5-methyl- >Pyridine, 4-(1-pyrrolidinyl)-

3

Pistachio Nuts

0.0E+00

1.0E+08

2.0E+08

3.0E+08

4.0E+08

5.0E+08

6.0E+08

7.0E+08

8.0E+08

9.0E+08

0 2 4 6 8 10 12 14

Time (Days)

Butanal, 3-methyl- Butanal, 2-methyl-

Hexanal Ethanol, 2-(2-ethoxyethoxy)-

3-Carene

0.0E+00

1.0E+08

2.0E+08

3.0E+08

4.0E+08

5.0E+08

6.0E+08

7.0E+08

8.0E+08

9.0E+08

0 2 4 6 8 10 12 14

Time (Days)

Butanal, 3-methyl- Butanal, 2-methyl-

Hexanal Ethanol, 2-(2-ethoxyethoxy)-

3-Carene

RIVM“MOBILE CHEMICAL & BACTERIOLOGICAL LABORATORY”

Dimensions: 38 cm x 39 cm x 23 cm

Weight: < 14.5 kg

Power: LiH battery

Carrier gas: Helium cartridge

Classic ‘Paul’ design

Reduce the size

Add central magnet

Diameter: 2 mm

Trap heater: 150-180°C

Vacuum: 2-stage turbo + diaphragm

Ionisation: 70 eV

Mass range: 45 – 500 Da

Scan rate: 10 scans/s

Technology: LTM

Column: MXT-5, 5 m x 0.1 mm x 0.4 µm

Carrier: Helium @ 25 psig (EPC)

Injection: S/SL with Merlin head

Oven: 50°C (10 s) to 300°C (10 s) @ 2°C/s

R2R time: 5.00 min

CUSTODION-SPME

“More robust configurationOf the classic SPME technique”

CUSTODION-NT

“Triple bed needle trap for dynamicsampling at low concentration”

CUSTODION-ADS

“Ideal for direct sampling ofvolatiles from surfaces”

START UP PROCEDURE

• Switch instrument ON• Wait for idle (2.00 minutes)• Inject QC standard• Pass/fail?• Start analyses

❶

❷

❸

Each datafile is processed automatically with the CHROMION software. The algorithm is proprietary andidentifies peaks based on spectral matching & retentionindex.

Deconvolution takes into account M+1 peaks.

DATA PROCESSING PROCEDURE

Key take aways

Easy-to-use field portable GC/MS system with a lot of potential in food-relatedapplications.

Joeri Vercammen+32-(0)10/45.00.25

[email protected]

www.is-x.com

More info?