Feeding and managing for maximum milk protein production Thomas R. Overton, Ph.D. Professor and Chair Department of Animal Science Director, PRO-DAIRY program Cornell University, Ithaca, NY DBM 0021b 1 Northeast Milk Price Forecasts 2019-2020 Accessed 5/20/20 at https://www.agrimark.c oop/PDFs/AM_Weekly _Updates.pdf 2
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Feeding and managing for maximum milk protein production
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Feeding and managing for maximum milk protein production
Thomas R. Overton, Ph.D.Professor and Chair
Department of Animal ScienceDirector, PRO-DAIRY programCornell University, Ithaca, NY DBM 0021b
• Dietary fatty acids– Unsaturated FA that modulate PPAR-alpha
Ibeagha-Awemu EM and Zhao X (2015) Front. Genet. 6:302. doi: 10.3389/fgene.2015.00302
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Summary of production responses to transition period AA
Study Treatment ResponseOverton et al., 1996 RPMet ↑ 2.7 kg/d FCMSocha et al., 2005 Met, Met+Lys ↑ 2.9 kg/d ECM for Met + LysPiepenbrink et al.,2004
HMTBa (13 g pre; 28 g post)HMTBa (27 g pre; 44 g post)
↑ 3.0 kg/d milkNS
Preynat et al., 2009; 2010
RPMet w/wo folic acid + B12
NS – milk yield↑ milk CP (2.94 vs. 3.04%)
Ordway et al., 2009 HMBiRPMet
No effect on milk yieldBoth trts ↑ milk protein %
Osorio et al., 2013 HMBiRPMet
↑ 3.8 kg/d ECM↑ 4.0 kg/d ECM
Batistel et al., 2017 RPMet ↑ 4.3 kg/d ECM
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Osorio et al., 2013. J. Dairy Sci. 96:6248-6263.Osorio et al., 2015. J. Dairy Sci. 97:7437-7450.Osorio et al., 2014. J. Dairy Sci. 97:7451-7464.
Osorio et al., 2016. J. Dairy Sci. 99:234-244.
• ~38 multiparous Holstein cows• Treatments (- 21 d pre to 30 days post)
– Control (Met ~ 1.8% of MP – NRC 2001)– HMBi at 0.19% of DM; 2.35% MP pre; 2.15% MP post – NRC 2001)– RP-Met at 0.07% DM; 2.38% MP pre; 2.15% MP post – NRC 2001)(Met
~ 2.2 to 2.3% MP – NRC 2001)• Lys ~ 6.6 to 6.7% MP prepartum; ~ 6.1 to 6.2% MP
postpartum (NRC 2001)
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Osorio et al., 2013. J. Dairy Sci. 96:6248-6263.Osorio et al., 2015. J. Dairy Sci. 97:7437-7450.Osorio et al., 2014. J. Dairy Sci. 97:7451-7464.
Osorio et al., 2016. J. Dairy Sci. 99:234-244.
• Cows fed Met pre- and postpartum– Tended to have greater neutrophil phagocytosis at 21 d postpartum– Lower plasma ceruloplasmin and serum amyloid A– Greater plasma oxygen radical absorbance capacity– Greater liver concentrations of glutathione and carnitine– Altered gene networks in liver consistent with altered oxidative
metabolism and inflammatory responses above– Greater methylation of PPAR-alpha promoter and upregulation of
associated pathways of lipid metabolism in liver
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• 60 multiparous Holstein cows• Treatments (- 28 d pre to 60 days post)
– Control (Met ~ 1.7% of MP – NRC 2001)– Met (Met ~ 2.2 to 2.3% MP – NRC 2001)
Effect of RP-Met supplementation during the periparturient period and early lactation on DMI and milk yield (Batistel et al., 2017. J. Dairy Sci. 100:7455-7487)
Variation in RUP and digestibility(Ross Unavailable N assay)
Source: Dairyland Labs
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Role of energy nutrition in milk protein synthesis
• Sporndly (1989) reported much stronger relationship of milk protein percentage with dietary energy intake than dietary protein intake– Often attributed to ruminal fermentation and
microbial protein synthesis– Sugars, starches, and digestible fiber sources
will drive microbial protein yield
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Effects of insulin on milk protein
• Hyperinsulinemic-Euglycemic clamps– Clamp alone
• 15% increase in milk protein yield (Mackle et al., 1999)
– Clamp w/ abomasal infusion of casein• 28% increase in milk protein yield (Griinari et al., 1997)
– Clamp w/ abomasal infusion of BCAA & casein• 25% increase in milk protein yield (Mackle et al., 1999)
– Clamp w/ IV infusion of AA (casein profile)• Insulin and insulin plus AA increased milk by 13 to 18% and
protein by 10 to 21% in goats– (Bequette et al, 2001)
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Long-acting insulins and milk protein• 30 multiparous Holstein cows
– 52 to 130 DIM, avg. 88 +/- 25• 3 treatments given at 12-h intervals for 10 d
– Control– 0.2 IU/kg of BW Humulin-N (Eli Lilly and Co.), 2X/d– 0.2 IU/kg of BW Insulin glargine (Sanofi-Aventis), 2X/d
• Blood samples– Twice daily from coccygeal vein– Before morning injections, 6 hours later
• Milk samples every other day, 2x/d
Winkelman and Overton, 2013. J. Dairy Sci. 96:7565-7577.
DMI, milk yield, and milk composition for cows administered two forms of long-acting insulin
Winkelman and Overton, 2013. J. Dairy Sci. 96:7565-7577.
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1.0
1.1
1.2
1.3
1.4
1.5
1.6
1.7
0 2 4 6 8 10 12
Day
Prot
ein
yiel
d, k
g/d
Control Humulin Insulin glargine
Effect of long-acting insulins on milk protein yield
Trt: P = 0.08Trt x Day: P = 0.08
C vs. I: P = 0.06H vs. L: P = 0.22
Winkelman and Overton, 2013. J. Dairy Sci. 96:7565-7577.
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0
2
4
6
8
10
12
14
16
0 2 4 6 8 10 12
Day
MU
N, m
g/dl
Control Humulin Insulin glargine
Trt: P = 0.01Trt x Day: P = 0.08
C vs. I: P = 0.004H vs. L: P = 0.61
Effect of long-acting insulins on MUN
Winkelman and Overton, 2013. J. Dairy Sci. 96:7565-7577.
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0
5
10
15
20
25
30
35
40
45
< C16 C16 > C16
FA c
ompo
sitio
n, g
/100
g F
A
Control H L
Milk FA composition for cows administered control (C) or two forms (H or L) of long-acting insulin. P < 0.05 for effects of treatment on < C16 and > C16. SEM = 0.9, 0.8, and 1.5 for < C16, C16, and > C16, respectively
Winkelman and Overton, 2013. J. Dairy Sci. 96:7565-7577.
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De novo FA content of milk is positively correlated with milk protein content on commercial dairy farms. From Barbano et al., 2014.
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Field implication – more glucogenic/propiogenic rations may
support greater responses to AA supplementation?
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Four treatments:ControlCN -- Duodenal infusion of casein (743 g/d)C3 -- Ruminal infusion of propionate (1,041 g/d)CN plus C3
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Raggio et al., 2006
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Four treatments:ControlCasein -- Abomasal infusion of casein (860 g/d)Starch – Abomasal infusion of starch (2 kg/d)Casein plus starch
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Item Water Casein Starch C + S SEM Starch Casein C x S