F.D.OVCHARENKO INSITUTE OF BIOCOLLOIDAL CHEMISTRY F.D.OVCHARENKO INSITUTE OF BIOCOLLOIDAL CHEMISTRY NATIONAL ACADEMY OF SCIENCES OF UKRAINE NATIONAL ACADEMY OF SCIENCES OF UKRAINE Kyiv Kyiv GOLD-BEARING PROBIOTIC DRUG GOLD-BEARING PROBIOTIC DRUG “OKARIN- “OKARIN- AU AU ” ” Valeriya VEMBER Valeriya VEMBER Telephone: +380-44-4241919 [email protected]Workshop “From Science to Business” 11-12 October, 2006 Kyiv
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F.D.OVCHARENKO INSITUTE OF BIOCOLLOIDAL CHEMISTRY NATIONAL ACADEMY OF SCIENCES OF UKRAINE Kyiv GOLD-BEARING PROBIOTIC DRUG OKARIN-AU Valeriya VEMBER Telephone:
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F.D.OVCHARENKO INSITUTE OF BIOCOLLOIDAL CHEMISTRY F.D.OVCHARENKO INSITUTE OF BIOCOLLOIDAL CHEMISTRY
NATIONAL ACADEMY OF SCIENCES OF UKRAINENATIONAL ACADEMY OF SCIENCES OF UKRAINE
KyivKyiv
GOLD-BEARING PROBIOTIC DRUG GOLD-BEARING PROBIOTIC DRUG “OKARIN-“OKARIN-AUAU””
M E T A L SM E T A L S── important regulatory environmental compounds andimportant regulatory environmental compounds and
potential ingredient for potential ingredient for SynbioticsSynbiotics
ADVISABILITYADVISABILITY of P of PROBIOTICROBIOTIC DRUG DRUG
ENRICHMENTENRICHMENT by by COLLOIDALCOLLOIDAL Au and Ag Au and Ag
Investigation of metals influence on some nonspecific physiological and biochemical parameters,
which have been of great importance for all the metabolic processes that take place in bacterial cell :
Growth processes’ intensity
Cell’s wall permeability
ATP-ase activity Energy function of bacterial plasma membrane
Level of lipid peroxidation Antioxidant enzymes activity
77
Changes of biological activity of bacterial Changes of biological activity of bacterial association “association “OkarinOkarin” after incubation with GOLD ” after incubation with GOLD
(10(10-6-6 mkgmkg//mlml) ) and SILVERand SILVER (10 (10-7-7 mkg/mlmkg/ml))
Metals Form
Investigated indexes
Growth intensity
ATP-ase activity
H+ -
extrusion
Perme-ability of the cell
wall
Level of lipid peroxi-
dation
Activity of antioxidant enzymes
Catalase SOD
AuColloidal 0
Ion 0 — — 0 0 0
AgColloidal 0 — — 0 0
Ion — —
Kind of changes : – positive; – negative; «—» – investigation is not conducted. 88
RResistance esistance of of “Okarin”“Okarin” and and“Okarin-Au” “Okarin-Au” to to the the antibioticantibioticss
99
Antagonistic activity of Antagonistic activity of bacterial association bacterial association ““OkarinOkarin””
and and gold-bearing gold-bearing ““Okarin-AuOkarin-Au””
Microorganisms(test object)
Size of lysis zone after 24 hours,
mm
Quantity of test-culture (pieces) (Х ± m)
ControlControl after 12 hoursafter 12 hours after 24 after 24 hourshours
* ─ behaviors, which show the improvement of initial preparation “Okarin” by colloidal gold. 1010
Wide range of antagonistic activity to pathogenic microorganisms causing dysbacteriosis and especially dangerous infection diseases including anthrax, rabbit-fever, salmonellosis, brucellosis etc. (according to results of clinical trials);
Selective bactericidal activity to pathogenic microflora and simultaneous stimulation of normoflora. This feature is intensified owing to the presence of colloidal gold particles;
Low cost of the treatment course in a comparison with other probiotic drugs which are available on the market today;
Ability to increase the lysis of atypical pathological cells treatment application opportunity for oncological patients due to high resistance of probiotic's microbial cultures to antibiotics and chematherapeutic agents;
Non-specific immune-resistance stimulation ability of the whole organism;
Suitable producing shape and application simplicity.
Innovative Aspect Innovative Aspect and Main Advantagesand Main Advantages
2) Prohibited to use at the same time with Antibiotics
1) Require preliminary measuring;
2) Prohibited to use at the same time with Milk and Dairy Produce
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• We seek for potential partners interested in new type of combined (and effective!) probiotic preparation and partners, who can help us in putting them on the market
• We are ready for collaboration with the purpose of creation of different synbiotic drugs which include wide range of colloidal metals and have different therapeutic effects
O p p o r t u n i t i e sO p p o r t u n i t i e s