GRC-BTS West 20th SoGAT Meeting, 12 - 13 June 2007, Warsaw 1 Evaluation data on the new Roche cobas s 201 system with the cobas TaqScreen MPX test Lutz Pichl , DRK-Blutspendedienst West Christine Jork, DRK-Blutspendedienst NSTOB Lutz Pichl, Volkmar Schottstedt German Red Cross Blood Transfusion Service West, Hagen, Germany
10
Embed
Evaluation data on the new Roche cobas s 201 system with the cobas TaqScreen MPX test
Lutz Pichl , DRK-Blutspendedienst West Christine Jork, DRK-Blutspendedienst NSTOB. Evaluation data on the new Roche cobas s 201 system with the cobas TaqScreen MPX test. Lutz Pichl, Volkmar Schottstedt German Red Cross Blood Transfusion Service West, Hagen, Germany. - PowerPoint PPT Presentation
Welcome message from author
This document is posted to help you gain knowledge. Please leave a comment to let me know what you think about it! Share it to your friends and learn new things together.
Transcript
GRC-BTS West 20th SoGAT Meeting, 12 - 13 June 2007, Warsaw 1
Evaluation data on the new Roche cobas s 201 system with the cobas TaqScreen MPX test
Lutz Pichl , DRK-Blutspendedienst West
Christine Jork, DRK-Blutspendedienst NSTOB
Lutz Pichl, Volkmar SchottstedtGerman Red Cross Blood Transfusion Service West,
Hagen, Germany
GRC-BTS West 20th SoGAT Meeting, 12 - 13 June 2007, Warsaw 2
Hagen
~ 4,000 - 6,000 samples/ day
GRC-BTS West 20th SoGAT Meeting, 12 - 13 June 2007, Warsaw 3
cobas s 201 system
GRC-BTS West 20th SoGAT Meeting, 12 - 13 June 2007, Warsaw 4
Aim of the study
evaluation of the Roche cobas s 201 System in combination with the TaqScreen MPX test
feasibility to screen plasma minipools(pools size 96 samples) for HCV, HIV-1,HIV-1 O, HIV-2 and HBVby multiplex PCR in a large blood bank setting
GRC-BTS West 20th SoGAT Meeting, 12 - 13 June 2007, Warsaw 5
Conditions
Fully automated, IVD-conf. , CE-marked system Focus on the performance of the MPX test
rather than on hardware features of the s 201(lab sample pooling unit and PCR unit with CAP/CTM systems have been operated since 2005 under routine screening conditions)
minipools are processed by the integrated Hamilton Microlab Star pipettor like single units
GRC-BTS West 20th SoGAT Meeting, 12 - 13 June 2007, Warsaw 6
Cross-Contamination Study
16 high titre, positive donor samples (5 HBV-DNA pos., 5 HCV-DNA pos., 5 HIV-1 pos.) and 16 non-reactive donor samples in alternating manner of 32 position on 2 racks
titres HBV: 8.0 EE8, HCV: 1.0 EE7, HIV-1: 1.0 EE6 2 different loading schemes were analysed result: no cross-contamination observed except
1 false (weak-) positive HCV (Ct 46.3 vs. Ct 18.4)
GRC-BTS West 20th SoGAT Meeting, 12 - 13 June 2007, Warsaw 7
Reproducibility
Reactive results/ tested replicatesDay 1 Day 2 Day 3 Day 4